CN114946846A - Method for treating ips typographus by applying push-pull strategy - Google Patents
Method for treating ips typographus by applying push-pull strategy Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M29/00—Scaring or repelling devices, e.g. bird-scaring apparatus
- A01M29/12—Scaring or repelling devices, e.g. bird-scaring apparatus using odoriferous substances, e.g. aromas, pheromones or chemical agents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P17/00—Pest repellants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
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Abstract
A method for treating ips typographus by applying a push-pull strategy belongs to the technical field of repelling pests by using chemical information substances. The ips typographus is controlled by combining the ips typographus attractant and the ips typographus repellent. The push-pull strategy applied by the method for controlling the ips typographus is to combine the ips typographus attractant and the ips typographus repellent, thereby not only reducing the cost, but also improving the control effect and being beneficial to large-scale forest application. The invention is environment-friendly, safe to natural enemies and beneficial organisms, does not pollute the environment, meets the desire of human beings on ecological safety, meets the current 'green' regulation and control and sustainable control targets for controlling forest pests, has the characteristics of wide sources, multiple varieties, lower price and the like, and has simple and quick technical operation and low requirement on human beings. Therefore, the method has high social, ecological and economic benefits.
Description
Technical Field
The invention belongs to the technical field of repelling pests by using chemical information substances, and particularly relates to a method for controlling ips typographus by applying a push-pull strategy.
Background
The term "push-pull" was originally proposed in 1987 by Pyke, Rice, Sabine and Zaluki in australia as a pest management strategy. The "push-pull" strategy of pest management is a new tool for pest integrated management programs, incorporating a combination of behavioral modification stimuli to control the distribution and quantity of pests and/or natural enemies. In this strategy, pests are repelled or prevented from (push) contact with major crops by the use of stimuli that mask the appearance of the host or have a repellent or deterrent effect. Pests are simultaneously attracted (pulled) to other areas, such as traps or trapped crops, using highly visible and attractive stimuli where they are concentrated for control. Based on the deep knowledge of the interaction among plants, pests and natural enemies, the 'push-pull' (push-pull) control measure formed by combining the trapping plants (crops) and the repelling plants or other chemical repelling substances becomes an important measure for a novel harmless comprehensive pest control method and pest ecological regulation. Currently, the "push-pull" strategy has a relatively large number of applications in the control of agricultural pests, such as controlling corn borers in africa, utilizing pennisetum and sudangrass as trap plants, while sugarcoated herbs and longhairy antenoron herbs as repelling plants to drive off spawning borers; and the prevention and control effects of different plant layouts (trapping treatment, repellent treatment and trapping-repellent treatment) on the Frankliniella occidentalis population show that the different plant layout treatments in the field have the regulation and control effects on the Frankliniella occidentalis nymphs and adult population dynamics. The push-pull strategy is applied to the control of agricultural pests, but is relatively rarely applied to the control of forestry pests.
Ips typographus Linnaeus belonging to Coleoptera, Scolytidae, Ips genus Ips, is widely distributed in the continental europe and is considered to be the most destructive species in coniferous forests to date. The natural plant damage agent mainly damages mature and over-mature spruce forest, can directly damage healthy standing trees when the insect damage is serious, and causes huge economic and ecological losses to forestry production and forest ecological environment. The previous treatment strategy for ips typographus mainly comprises the following steps: physical control (felling and removing victims, trapping and killing bait trees and the like), chemical control (punching, injecting drugs, releasing cigarettes and the like), natural enemy control (releasing, attracting parasitic or predatory natural enemies), forest culture and management measures (building mixed forests, strong seedlings and the like), and along with the rise and rapid development of chemical ecology and molecular technology, the application of the pheromone attractant to monitor and control the spruce ips among forests becomes a preferred method in the field of the management of the spruce ips because of the advantages of low cost, good effect, environmental friendliness, reduction of environmental pollution and damage caused by the use of chemical pesticides and the like. Although the single application of the attractant reduces the population density of forest pests to a certain extent, the population density of ips typographus can still cause a large number of host plants to die in a forest ecosystem which is endangered all the year round. Therefore, management only from the perspective of a large number of trapping and killing still has some disadvantages, the search for an efficient pollution-free efficient population control technology is particularly important for protecting host plants, and pest management by using a push-pull strategy is an effective technical means for realizing the aim, and the pest management can play a role in controlling pests with half the effort.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to design and provide a technical scheme of a method for treating ips typographus by applying a push-pull strategy.
The invention is realized by the following technical scheme:
the invention provides a method for controlling ips typographus by applying a push-pull strategy, which prevents ips typographus by combining an ips typographus attractant and an ips typographus repellent.
Further, a spruce ips repellent is used around the host trees to expel pests, so that the host plants are prevented from being damaged, and meanwhile, a spruce ips attractant is used for trapping and killing to prevent and control the pests at the forest edge of damage.
Further, the ips typographus attractant comprises the following components in parts by weight:
cis-verbenol 1.5-120 weight portions
1.5-120 parts of 2-methyl-3-buten-2-ol
1.5-120 parts of alpha-pinene (+25/-75)
0.001-0.02 part of 2, 6-di-tert-butyl-p-cresol
0.001 to 0.02 portion of 2-hydroxy-4-methoxybenzophenone.
Further, the ips typographus repellent comprises the following components in parts by volume:
1, 8-cineole 1-100 parts
1-100 parts of 3-carene.
Further, the ips typographus attractant comprises the following components in parts by weight:
cis-verbenol 10-60 weight portions
10-60 parts of 2-methyl-3-butene-2-ol
10-90 parts of alpha-pinene (+25/-75)
0.01-0.02 part of 2, 6-di-tert-butyl-p-cresol
0.01-0.02 part of 2-hydroxy-4-methoxybenzophenone.
Further, the ips typographus repellent comprises the following components in parts by volume:
1, 8-cineole 5-50 parts
5-50 parts of 3-carene.
The preparation process of the attracting core of ips typographus attractant comprises the following specific steps:
1) preparation of a slow release bottle: soaking polyethylene slow release bottle (purchased from Beijing Zhongjie Siamese Biotech Co., Ltd.) in ethanol for 5-10 days, washing the lure carrier with distilled water until no ethanol smell exists, air drying, bagging and sealing for later use;
2) preparing an attractant: accurately weighing the effective components in the formula in milligrams as units, and then vibrating and mixing the effective components on a homogenizer at a low speed for later use;
3) dripping the attractant prepared in the step 2) into the core-attracting carrier prepared in the step 1), and dripping 50-300 mu L of dichloromethane into each attracting core in the core-attracting carrier to obtain the attracting core of the ips typographus attractant.
The preparation process of the ips typographus repellent comprises the following specific steps:
1) preparing a sustained-release bag: soaking the degradable sustained-release bag in ethanol for 1-3 days, washing with distilled water until no ethanol smell exists, and air drying;
2) preparing a repellent: the parts of the effective components in the formula are accurately weighed by taking milliliters as a unit for later use;
3) after media such as absorbent cotton or gauze are added into the slow release bag, the repellent prepared in the step 2) is respectively dripped into the core-attracting carrier prepared in the step 1), and the adult ips typographus repellent is prepared.
The ips typographus attractant adopted by the invention can be specifically seen in an attractant patent of ips typographus applied by the inventor, the publication number is CN105746511A, and the application number is 201610183543.0.
The push-pull strategy applied by the method for controlling the ips typographus is to combine the ips typographus attractant and the ips typographus repellent, thereby not only reducing the cost, but also improving the control effect and being beneficial to large-scale forest application. The invention is environment-friendly, safe to natural enemies and beneficial organisms, does not pollute the environment, meets the desire of human beings on ecological safety, meets the current 'green' regulation and control and sustainable control targets for controlling forest pests, has the characteristics of wide sources, multiple varieties, lower price and the like, and has simple and quick technical operation and low requirement on human beings. Therefore, the method has high social, ecological and economic benefits.
Drawings
FIG. 1 shows the trapping amount of ips typographus adults of each example;
FIG. 2 is a schematic diagram of a combined configuration of a forest attractant and a repellent;
FIG. 3 is a comparison of attractant use with repellent versus attractant trapping.
Detailed Description
The present invention is further illustrated by the following examples.
Example 1: preparation of spruce bark beetle repellent (volume)
Weighing 1 part of 1, 8-cineole and 1 part of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 2: preparation of spruce bark beetle repellent (volume)
Weighing 5 parts of 1, 8-cineole and 5 parts of 3-carene, and respectively putting the weighed materials into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 3: preparation of spruce bark beetle repellent (volume)
Weighing 10 parts of 1, 8-cineole and 10 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 4: preparation of spruce bark beetle repellent (volume)
Weighing 100 parts of 1, 8-cineole and 100 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 5: preparation of spruce bark beetle repellent (volume share)
Weighing 10 parts of 1, 8-cineole and 1 part of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 6: preparation of spruce bark beetle repellent (volume)
Weighing 5 parts of 1, 8-cineole and 1 part of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 7: preparation of spruce bark beetle repellent (volume)
Weighing 1 part of 1, 8-cineole and 5 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Example 8: preparation of spruce ips attractant (parts by weight)
Weighing 1.5 parts of cis-verbenol, 1.5 parts of 2-methyl-3-buten-2-ol, 1.5 parts of alpha-pinene (+25/-75), 0.001 part of 2, 6-di-tert-butyl-p-cresol and 0.001 part of 2-hydroxy-4-methoxybenzophenone, and uniformly mixing on an oscillator to obtain the ips typographus imago attractant.
Example 9: preparation of spruce ips attractant (parts by weight)
Weighing 10 parts of cis-verbenol, 10 parts of 2-methyl-3-buten-2-ol, 10 parts of alpha-pinene (+25/-75), 0.01 part of 2, 6-di-tert-butyl-p-cresol and 0.01 part of 2-hydroxy-4-methoxybenzophenone, and uniformly mixing on an oscillator to obtain the adult ips typographus attractant.
Example 10: preparation of spruce bark beetle attractant (parts by weight)
Weighing 30 parts of cis-verbenol, 30 parts of 2-methyl-3-buten-2-ol, 90 parts of alpha-pinene (+25/-75), 0.005 part of 2, 6-di-tert-butyl-p-cresol and 0.005 part of 2-hydroxy-4-methoxybenzophenone, and uniformly mixing on an oscillator to obtain the adult ips typographus attractant.
Example 11: preparation of spruce ips attractant (parts by weight)
Weighing 60 parts of cis-verbenol, 60 parts of 2-methyl-3-buten-2-ol, 60 parts of alpha-pinene (+25/-75), 0.02 part of 2, 6-di-tert-butyl-p-cresol and 0.02 part of 2-hydroxy-4-methoxybenzophenone, and uniformly mixing on an oscillator to obtain the adult ips typographus attractant.
Example 12: preparation of spruce ips attractant (parts by weight)
Weighing 120 parts of cis-verbenol, 120 parts of 2-methyl-3-buten-2-ol, 100 parts of alpha-pinene (+25/-75), 0.001 part of 2, 6-di-tert-butyl-p-cresol and 0.001 part of 2-hydroxy-4-methoxybenzophenone, and uniformly mixing on an oscillator to obtain the adult ips typographus attractant.
Comparative example 1
Weighing 1 part of 1, 8-cineole, and putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Comparative example 2
And weighing 5 parts of 3-carene, and putting the 3-carene into a slow release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Comparative example 3
Weighing 4 g of myrcene, and putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Comparative example 4
Weighing 4 g of nonanal, and putting into a slow-release bag filled with absorbent cotton to obtain the adult ips typographus repellent.
Test example 1: the ips typographus repellents prepared in examples 1, 2, 3 and 5 and comparative examples 1, 2, 3 and 4 were respectively hung together with an attractant for ips typographus (accurately weighed in milligrams using the ips typographus attractant prepared in example 11) at the connecting rod of a funnel-type trap. Traps were hung in the 36 forest shifts of the kingdom greens forest farm of the wanqing forest office at a 100m spacing, with each treatment and repetition randomly distributed therein. The height of the trapper hung between two trees is about 2m, the distance between the lower edge of the trapper and the ground is about 50cm, and the hanging time is from the middle ten days of 4 months to the beginning of 8 months. Each treatment was 5 replicates. The data analysis adopts a one-factor analysis of variance method and Duncan's double-range test.
As can be seen from the survey data in table 1 and fig. 1, the repellent according to the present invention significantly reduced the trapping effect of the attractant.
Table 1: trapping effect of various embodiments of Wanqing forest industry bureau of Jilin province in 2020
| Different embodiments | Trapping amount + -Standard error | Significance of difference |
| Attractant of patented formula | 4611.4±660.17 | a |
| Comparative example 2 | 4499.2±761.32 | a |
| Comparative example 3 | 1874.8±446.56 | b |
| Comparative example 4 | 1542.2±126.69 | b |
| Comparative example 1 | 1528.8±67.3 | b |
| Example 1 | 356.8±156.71 | c |
| Example 2 | 297.2±50.41 | c |
| Example 3 | 171.8±21.73 | c |
| Example 5 | 285±71.85 | c |
| Blank control | 0.4±0.24 | c |
Note: different letters represent significant differences at the 0.05 level.
Test example: two sample fields are selected as a control field and a test field in 36 forest classes of the Jingouling mountain forest farm of the Wanqing forest industry office by the advantages of environment-friendly and sustainable control of the push-pull technology and the like in combination with the efficacy and the non-toxic principle, and the specific setting is as shown in figure 2. The insects in the trap insect collecting barrel are collected once every 10 days, and 10 times are collected in total. The repellent of example 3 was combined with the attractant of example 11.
The results of the statistical analysis show that: the average trapping amount of the attractant and the repellent combined was 3237.25 + -934.51, which is significantly higher than the trapping amount of 2331.5 + -582.88 of the control group using only attractant (fig. 3). The push-pull technology of using attractant to trap and kill the spruce ips around the host trees and using repellent to prevent and control the spruce ips effectively reduces the population density in the forest, and the trapping effect is obviously different from the control. The repellent is combined with the attractant for use, is suitable for the comprehensive treatment of ips typographus with large occurrence area and complex terrain and topography, and achieves the dual functions of controlling the species group of ips typographus and protecting host plants by using the push-pull technology of ips typographus to prevent and control the damage of ips typographus.
According to the push-pull strategy applied by the invention, the trapping effect is improved by 38.9% compared with that of a single attractant, and the trapping amount is obviously different. From the economic cost, the cost of the attractant is basically 40 yuan per piece, the cost of the repellent is basically 10 yuan per piece, and the economic cost is saved by 23 percent. If the push-pull strategy is applied in a large scale to prevent ips typographus, the economic cost is further reduced.
Claims (6)
1. A method for treating ips typographus by applying a push-pull strategy is characterized in that the ips typographus attractant and the ips typographus repellent are used together to prevent and treat the ips typographus.
2. The method for controlling ips typographus using push-pull strategy as claimed in claim 1, wherein the ips typographus repellent is used around the host tree to repel the pests and avoid the host plant from being damaged, and the ips typographus attractant is used to trap and kill the pests at the forest edge of the damage to prevent and control the pests.
3. The method for controlling ips typographus using a push-pull strategy as claimed in claim 1 or 2, wherein the ips typographus attractant comprises the following components in parts by weight:
cis-verbenol 1.5-120 weight portions
1.5-120 parts of 2-methyl-3-buten-2-ol
1.5-120 parts of alpha-pinene (+25/-75)
0.001-0.02 part of 2, 6-di-tert-butyl-p-cresol
0.001 to 0.02 portion of 2-hydroxy-4-methoxybenzophenone.
4. The method for controlling ips typographus using a push-pull strategy as claimed in claim 1 or 2, wherein said ips typographus repellent comprises the following components in parts by volume:
1, 8-cineole 1-100 parts
3-carene 1-100 parts.
5. The method for controlling ips typographus using a push-pull strategy as claimed in claim 3, wherein said ips typographus attractant comprises the following components in parts by weight:
cis-verbenol 10-60 weight portions
10-60 parts of 2-methyl-3-butene-2-ol
10-90 parts of alpha-pinene (+25/-75)
0.01-0.02 part of 2, 6-di-tert-butyl-p-cresol
0.01-0.02 part of 2-hydroxy-4-methoxybenzophenone.
6. The method for management of ips typographus using a push-pull strategy as claimed in claim 4, wherein said ips typographus repellent comprises the following components in parts by volume:
1, 8-cineole 5-50 parts
5-50 parts of 3-carene.
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Citations (4)
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|---|---|---|---|---|
| CN105746511A (en) * | 2016-03-28 | 2016-07-13 | 程彬 | Attractant for ips typographus |
| CN111165490A (en) * | 2020-01-14 | 2020-05-19 | 中国林业科学研究院森林生态环境与保护研究所 | Application of information chemical substance in repelling ips typographus and repellent slow-release bag and application thereof |
| JP2021165303A (en) * | 2021-07-07 | 2021-10-14 | 住友化学株式会社 | Pest control composition and pest control method |
| CN116195589A (en) * | 2022-06-20 | 2023-06-02 | 吉林省林业科学研究院 | Repellent for bark beetle adults in spruce and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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