CN111165490A

CN111165490A - Application of information chemical substance in repelling ips typographus and repellent slow-release bag and application thereof

Info

Publication number: CN111165490A
Application number: CN202010036891.1A
Authority: CN
Inventors: 孔祥波; 方加兴; 张苏芳; 刘福; 张真
Original assignee: Research Institute of Forest Ecology Environment and Protection of Chinese Academy of Forestry
Current assignee: Research Institute of Forest Ecology Environment and Protection of Chinese Academy of Forestry
Priority date: 2020-01-14
Filing date: 2020-01-14
Publication date: 2020-05-19
Anticipated expiration: 2040-01-14
Also published as: CN111165490B

Abstract

The invention provides an application of an information chemical substance in repelling ips typographus, belonging to the technical field of biological control of ips typographus, wherein the information chemical substance comprises myrcene and/or nonanal. In the invention, the semiochemical comprises myrcene and/or nonanal and has a repellent effect on ips typographus.

Description

Application of information chemical substance in repelling ips typographus and repellent slow-release bag and application thereof

Technical Field

The invention belongs to the technical field of biological control of ips typographus, and particularly relates to application of an information chemical substance in repelling ips typographus, a repellent slow-release bag and application thereof.

Background

Ips typographus L is the most harmful trunk-boring pest in the picea europe, mainly harms the phloem, can cause the host to die in a short time, and other secondary pests continue to invade the damaged trees in the host death process, thereby accelerating the death speed of the host. Along with the change of global climate, the phenological and chemical properties of ips typographus are greatly changed, the population expansion is obvious, the harm is gradually serious, even the ips typographus can invade healthy spruce and cause death when the population density is high, the ips typographus causes the damage which is difficult to recover to the natural spruce forest in China, and the serious and lasting economic and ecological loss of the spruce forest is caused. The monitoring and control of the ips typographus are successfully realized by utilizing the aggregation pheromone of the ips typographus, and the aggregation pheromone component is a binary compound combination comprising 2-methyl-3-butene-2-ol and S-cis-verbenol. Although the application of the aggregation pheromone can reduce the population density of ips typographus pests in forests to a certain extent and reduce the damage of ips typographus pests to spruce forests, the population density of ips typographus still can cause a large amount of spruce deaths in spruce ecosystems which are damaged all the year round. Therefore, the management strategy for realizing the ips typographus insect pest only from the perspective of trapping and killing a large number of spruce still has some defects, and the search for the efficient and pollution-free population repellent has important ecological and economic significance for protecting the spruce forest.

Disclosure of Invention

In view of the above, the invention aims to provide an application of an information chemical substance in repelling ips typographus and a repellent slow-release bag and an application thereof.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides an application of semiochemicals in repelling ips typographus, wherein the semiochemicals comprise myrcene and/or nonanal.

The invention also provides a repellent slow-release bag which comprises the information chemical substance, the absorbent cotton and the polyethylene plastic bag in the technical scheme.

Preferably, the ratio of the mass of the semiochemicals to the number of the polyethylene plastic bags is 1-16 g: 1.

Preferably, the mass ratio of the absorbent cotton to the polyethylene plastic bags is 2-3 g: 1.

Preferably, the method further comprises the following steps: antioxidants and ultraviolet light absorbers.

Preferably, the antioxidant comprises butyl hydroxyanisole or 2, 6-di-tert-butyl p-cresol, and the ultraviolet light absorber comprises 2-hydroxy-4-methoxybenzophenone.

Preferably, the ratio of the mass of the antioxidant to the number of the polyethylene plastic bags is 5-10 mg: 1.

Preferably, the ratio of the mass of the ultraviolet light absorber to the number of the polyethylene plastic bags is 5-10 mg: 1.

The invention also provides application of the repellent sustained-release bag in the technical scheme in repelling ips typographus.

Preferably, the application comprises: and (3) hanging the repellent slow-release bags in the forest, wherein the number of hung repellent slow-release bags per mu is 5-8.

The invention provides an application of semiochemicals in repelling ips typographus, wherein the semiochemicals comprise myrcene and/or nonanal. In the invention, the semiochemical comprises myrcene and/or nonanal and has a repellent effect on ips typographus.

Drawings

FIG. 1 is a behavior regulation test of 3 plant-derived volatiles on ips typographus;

FIG. 2 is a behavior inhibition test of ips typographus with varying amounts of myrcene;

FIG. 3 shows the behavior inhibition test of ips typographus with different nonanal content;

FIG. 4 is a behavioral inhibition assay for ips typographus using geranene and nonanal together.

Detailed Description

The invention provides an application of semiochemicals in repelling ips typographus, wherein the semiochemicals comprise myrcene and/or nonanal. The sources of the myrcene and the nonanal are not particularly limited, and the myrcene and the nonanal can be obtained by adopting conventional commercial products. The purities of the myrcene and the nonanal are both more than 95%.

In the invention, the ratio of the mass of the semiochemical to the number of the polyethylene plastic bags is preferably 1-16 g:1, specifically 16g:1, 8g:1, 4g:1, 2g:1 and 1g: 1. In the present invention, when the semiochemical includes myrcene and nonanal, the myrcene and the nonanal are mixed in an arbitrary mass ratio, preferably 1: 1. In the invention, the ratio of the mass of the absorbent cotton to the number of the polyethylene plastic bags is preferably 2-3 g: 1. In the invention, the polyethylene plastic bag is preferably a 12s black polyethylene plastic bag, and contains 0.4 percent (W percent) of antioxidant 168, 0.6 percent (W percent) of dioctyl biphenyl dicarboxylate, 99 percent (W percent) of low-density polyethylene, 130mm in length and 70mm in width.

In the present invention, the repellent sustained-release pouch further preferably includes an antioxidant and an ultraviolet light absorber. In the present invention, the antioxidant preferably comprises butyl hydroxyanisole or 2, 6-di-tert-butyl-p-cresol, and the ultraviolet light absorber preferably comprises 2-hydroxy-4-methoxybenzophenone. In the invention, the mass ratio of the antioxidant to the number of the polyethylene plastic bags is preferably 5-10 mg: 1; the mass ratio of the ultraviolet absorbent to the number of the polyethylene plastic bags is preferably 5-10 mg: 1.

In the present invention, the preparation method of the repellent sustained-release bag preferably includes: and adding the information chemical substance into absorbent cotton, putting the absorbent cotton into a polyethylene plastic bag, and sealing. In the present invention, the absorbent cotton is preferably medical absorbent cotton.

In the invention, when the repellent is applied to large areas in forests to prevent and control ips typographus spruce, the application preferably comprises the step of directly hanging the repellent slow-release bag on a forest spruce trunk by a thumbtack, wherein the number of the hanging bags per mu is 5-8, and more preferably 6; the distance between every two repellent sustained-release bags is preferably 40-60 m, and more preferably 50 m. In the present invention, the repellent distance of the repellent sustained-release bag is >100 m. In the invention, the polyethylene plastic bag is not required to be opened, and the information chemical substances can be directly released through the bag wall of the plastic bag. In the invention, the effective period of the repellent sustained-release bag is preferably 60-70 days.

When the repellent is applied to large areas in forests to prevent and control ips typographus, the repellent slow-release bag is preferably matched with the attracting core of the ips typographus attractant and the ips trap for use, and the damage of the ips typographus to spruce forests is prevented and controlled by adopting a push-pull control strategy.

The beetle trap is commercially available in a conventional mode, in the invention, the trap is preferably arranged at the forest edge in a configuration method in a push-pull prevention and control technology, and the distance between traps is preferably 50-70 m, and more preferably 60 m. In the invention, the trapper is mainly a cross plate type bark beetle trapper which is purchased from Beijing Zhongjie tetragonal Biotechnology GmbH, large plates and small plates are crossed to form a cross shape (with the height of 500mm and the width of 300mm), the cross plate type bark beetle trapper is connected with an upper cover through a hook at the top of a cross baffle plate, is connected with a funnel through a hook at the bottom of the cross baffle plate, and the funnel is connected with a pest collecting bottle.

In the invention, the attracting core of the ips typographus attractant comprises 2-methyl-3-buten-2 ol, S-cis-verbenol, an antioxidant, an ultraviolet light absorber and polyvinyl chloride plastic cloth. In the invention, the mass of 2-methyl-3-buten-2 ol in each sustained-release bag made of polyethylene plastic cloth is preferably 4g, the mass of S-cis-verbenol is preferably 40mg, the mass of an antioxidant is preferably 5-10 mg, and the mass of an ultraviolet absorbent is preferably 5-10 mg. In the invention, the slow release bag made of the polyvinyl chloride plastic cloth preferably contains 60 mass percent of polyvinyl chloride and 26 mass percent of dioctyl phthalate, the length of the polyvinyl chloride plastic bag is preferably 90mm, and the width of the polyvinyl chloride plastic bag is preferably 40 mm. In the present invention, the antioxidant preferably comprises butyl hydroxyanisole or 2, 6-di-tert-butyl-p-cresol, and the ultraviolet light absorber preferably comprises 2-hydroxy-4-methoxybenzophenone.

In the invention, the preparation method of the attracting core of ips typographus aggregation attractant preferably comprises the following steps: adding the 2-methyl-3-buten-2 ol, the S-cis-verbenol, the antioxidant and the ultraviolet absorber into a polyvinyl chloride plastic cloth slow-release bag, and sealing to obtain the ips typographus aggregation pheromone attractant core.

In the invention, in order to evaluate the repellent effect of myrcene and/or nonanal which are information chemical substances of ips typographus, a repellent slow-release bag prepared from myrcene and/or nonanal and an aggregation pheromone attractant core of ips typographus are used simultaneously. The purpose of using the ips typographus aggregation pheromone attractant core alone was to serve as a control. When the insect trap is used, the repellent slow release and the attractant attracting core are simultaneously placed in the middle of the cross baffle of a bark beetle trap, and then the trap is hung among 2 spruces in a forest (hung by iron wires). The trapper woodland is designed in a random block mode, the distance between the trappers in the block is preferably 40-60 m, more preferably 50m, and the distance between the trappers in the block is preferably 200 m. The good or bad repellent effect of the repellent slow-release bag is judged according to the trapping quantity of the ips typographus in the trap insect collecting bottle.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

in particular, in preliminary experiments, it has been clarified that 2-methyl-3-buten-2 ol, S-cis-verbenol, myrcene, nonanal and (-) - β -pinene used alone in forests cannot trap ips spruce ips typographus.

Example 1

Preparing a ips typographus repellent sustained-release bag: the chemical purity and the stereoisomer purity of the myrcene purchased from the market are both more than 95 percent. 4g of myrcene, 5mg of butyl hydroxyanisole and 5mg of 2-hydroxy-4-methoxybenzophenone are mixed to prepare the repellent. Then taking a black polyethylene plastic bag purchased in the market as a carrier, transferring and dripping the repellent into the absorbent cotton in the black polyethylene plastic bag carrier by using the prepared repellent by using a liquid transfer device to prepare the spruce ips typographus repellent sustained-release bag.

Preparing a luring core of the ips typographus aggregation pheromone attractant: uniformly mixing 4g of 2-methyl-3-buten-2 ol, 40mg of S-cis-verbenol, 5mg of butyl hydroxy anisole and 5mg of 2-hydroxy-4-methoxybenzophenone, adding the mixture into a slow-release bag made of polyvinyl chloride plastic cloth by using a pipette, and sealing to obtain the ips aggregation pheromone attractant core.

The method is used for evaluating the repellent effect of the repellent on ips typographus by applying the core luring agent and the repellent sustained-release bag of the ips typographus aggregation pheromone attractant, and comprises the following specific steps:

manufacturing the trap: the trap is mainly a cross plate type bark beetle trap which is purchased from Beijing Zhongjie tetragonal Biotechnology GmbH. The trap is assembled according to the instructions for use. The big plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300mm), the hook at the top of the cross baffle is connected with the upper cover, the hook at the bottom of the cross baffle is connected with the funnel, and the funnel is connected with the insect collecting bottle. The trap was suspended between 2 spruce trees with iron wire and the trap bottle was about 100 cm from the ground. If necessary, the upper cover, baffle, trap bottle, bottom funnel of the bark beetle trap may be reinforced with iron wire to ensure that the trap is not damaged by adverse weather conditions during the test.

1) Suspension of the repellent sustained-release bag and/or the attractant core: the trapper is used for comparative examples 1, 3 and 4, and comparative example 5, wherein 1 piece of repellent slow-release bag and 1 piece of attractant core are hung in the middle of the cross-shaped baffle of the trapper, and the trapper only hanging 1 piece of attractant core is hung and fixed by thin iron wires.

2) The field setting of trapper: 3 replicates were set for each test treatment, traps were in a random block design in the forest, the intra-block trap spacing was approximately 50 meters, the inter-block trap spacing was 200 meters, the traps were hung between 2 trees with wire, and trap bottles were approximately 100 centimeters high from the ground.

3) Data investigation and trap emptying: correctly hanging the trap according to requirements when the adult ips typographus is opened in the flying period, surveying and recording the number of the ips typographus in the trap bottle and the weather condition of the day every 2-3 days by investigators, and meanwhile, putting the attracted ips typographus into a plastic box to be taken away.

In the experiment of 5 months in 2019, the traps are spaced at 50m, hung among 2 trees, and the height of an insect collecting bottle is about 100 cm above the ground, each treatment is set to be repeated for 3 times, and the number of the beetles caught by each treatment is the average number of the single traps. According to the weather conditions, the number of bark beetles in the bark beetle collecting bottle is observed every 2-3 days, and the result is shown in figure 1.

Example 2

The active substance in the delayed release bags of ips typographus repellent is nonanal, the dosage of each delayed release bag is 4g, and the rest is the same as the example 1. The results are shown in FIG. 1.

Comparative example 1

Only the ips typographus aggregation pheromone attractant core is prepared, the preparation method and the use amount are the same as the example 1, and the rest is the same as the example 1. The results are shown in FIG. 1.

Comparative example 2

The ips typographus aggregation pheromone attractant core: the attractants were 2-methyl-3-buten-2 ol and S-cis-verbenol, prepared in the same manner and in the same amounts as in example 1.

preparation of (-) - β -pinene sustained release bags, sample dosage of each sustained release bag, and kinds and dosages of antioxidant and ultraviolet absorbent were the same as those of the myrcene repellent sustained release bag in example 1, and the results are shown in fig. 1.

from the data analysis in fig. 1 (each observation shows that the average number of ips typographus per trap is average), the induction core of ips typographus aggregation pheromone has very good induction effect (comparative example 1). the (-) - β -pinene slow-release bag has no significant synergistic effect or inhibition effect on the comparative example 1, 2 treatments induce the induction core to induce ips typographus with no significant difference in effect (comparative example 2). when the myrcene slow-release bag or the nonanal slow-release bag is used together with the induction core of ips typographus aggregation pheromone, both the two components have strong effect on the induction core of the attractant, the number of ips typographus in the trap is very small, the 2 components can reduce the induction core induction amount of ips typographus with 5.4 times to 24 times (example 2) of the induction core of ips typographus aggregation pheromone, and the 2 components can show that the induction core of ips typographus with no significant inhibition effect (example 1) and the induction core of ips typographus only 2 can achieve the effect of inhibiting aggregation of the induction of the ips typographus only 96 percent, and the induction core of ips typographus only can show the combined inhibition effect of the induction core of the ips typographus when the induction core of the ips typographus pinus with no inhibition effect (example 2).

Example 3

The active substance in the delayed release bag of ips typographus repellent is myrcene with the dosage of 1g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The active substances in the luring core of the ips typographus aggregation pheromone attractant are 2-methyl-3-buten-2 ol and S-cis-verbenol, and the dosage and the preparation method are the same as the example 1.

The use of the repellent slow-release bag and the collective attractant slow-release bag was the same as in example 1, and the other conditions were the same as in example 1.

In 6 months of 2019, the ips typographus gathering pheromone attractant core and the repellent slow-release bag are placed in a forest, the distance between traps is 50m, the ips typographus gathering pheromone attractant core and the repellent slow-release bag are hung between 2 trees and 100 centimeters above the ground, each treatment is repeated for 3 times, and the number of the ips typographus caught by each treatment is the average number of 3 traps. Observing the number of the bark beetles in the bark beetle collecting bottle for 1 time every 2-3 days according to weather conditions, and placing the collected bark beetles into a plastic box to be brought to a laboratory after counting. The results are shown in FIG. 2.

Example 4

The active substance in the delayed release bag of ips typographus repellent is myrcene, the dosage is 2g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The same conditions as in example 1 were used for the sustained-release bag of the repellent and the lure of the aggregation pheromone attractant, and the other conditions were the same as in example 1. The results are shown in FIG. 2.

Example 5

The active substance in the delayed release bag of ips typographus repellent is myrcene, the dosage is 4g, and the preparation method of the delayed release bag of the repellent is the same as that of the example 1.

The use of the repellent sustained release bag and aggregation pheromone attractant core is the same as in example 1, and the other conditions are the same as in example 1. The results are shown in FIG. 2.

Example 6

The active substance in the delayed release bag of ips typographus repellent is myrcene, the dosage is 8g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The use of the repellent sustained release bag and the attractant core of the aggregation attractant is the same as in example 1, and the rest conditions are the same as in example 1. The results are shown in FIG. 2.

Example 7

The active substance in the delayed release bag of ips typographus repellent is myrcene, the dosage is 16g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

Comparative example 3

The ips typographus aggregation pheromone attractant core: the attractants are 2-methyl-3-buten-2 ol and S-cis-verbenol, and the preparation method and the using amount are the same as those in example 1, and the rest conditions are the same as those in example 1. The results are shown in FIG. 2.

From the trap data analysis in fig. 2 (each observation averaging the number of ips typographus per trap), the ips typographus aggregation pheromone attractant core had very good trapping effect (comparative example 3), with an average of 16.4 traps per trap. When the dosage of the myrcene in each repellent slow-release bag is more than 4g, the inhibition effect is optimal, and the inhibition rate reaches 84.8% (example 5) to 92.7% (example 6 and example 7); when the dosage of the myrcene in each repellent slow-release bag is 1-2 g, compared with comparative example 3, the slow-release bag also has a remarkable inhibition effect, and the inhibition rate reaches 66.5% (example 3) -78% (example 4). The myrcene can be used as a repellent of ips typographus to be independently applied among forests or be combined with aggregation pheromone to comprehensively prevent and control the damage of ips typographus.

Example 8

The active substance in the delayed release bag of ips typographus repellent is nonanal with the dosage of 1g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The use of the repellent sustained release bag and aggregation pheromone attractant core is the same as in example 1, and the other conditions are the same as in example 1.

In 6 months of 2019, the ips typographus aggregation pheromone attractant and the repellent slow-release bag are placed in a forest, the distance between traps is 50m, the ips typographus aggregation pheromone attractant and the repellent slow-release bag are hung between 2 trees, the distance between an insect collecting bottle and the ground is about 100 cm, each treatment is repeated for 3 times, and the number of the ips typographus induced by each treatment is the average number of 3 traps. Observing the number of the bark beetles in the bark beetle collecting bottle for 1 time every 2-3 days according to weather conditions, and putting the collected bark beetles into a plastic box to be carried to a laboratory after counting, wherein the result is shown in figure 3.

Example 9

The active substance in the delayed release bag of ips typographus repellent is nonanal, the dosage is 2g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The use of the repellent sustained release bag and aggregation pheromone attractant core is the same as in example 1, and the other conditions are the same as in example 1. The results are shown in FIG. 3.

Example 10

The active substance in the delayed release bag of ips typographus repellent is nonanal, the dosage is 4g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

Example 11

The active substance in the delayed release bag of ips typographus repellent is nonanal, the dosage is 8g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

Example 12

The active substance in the delayed release bag of ips typographus repellent is nonanal, the dosage is 16g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

Comparative example 4

The ips typographus aggregation pheromone attractant core: the attractants are 2-methyl-3-buten-2 ol and S-cis-verbenol, and the preparation method and the using amount are the same as those in example 1, and the rest conditions are the same as those in example 1. The results are shown in FIG. 3.

From the data analysis of the trapping data in fig. 3 (each observation of the average number of ips typographus per trap), the ips typographus aggregation pheromone attractant alone had very good trapping effect, and the average trapping amount per trap was 17.8 (comparative example 4). When the dosage of nonanal in each repellent slow-release bag is more than 4g, the inhibition effect is optimal, the inhibition rate reaches over 86% (examples 10-12), and compared with comparative example 4, the repellent effect is remarkable. Examples 8 and 9 also have remarkable repellent effects, and the repellent rate reaches 60% (example 8) to 80% (example 9). The pelargonic can be used as a repellent of ips typographus in forests independently or in combination with aggregation pheromone, so that the damage of ips typographus can be comprehensively prevented and controlled.

Example 13

The active substances in the delayed release bag of the ips typographus repellent are nonanal and myrcene, the dosage is 2g, and the preparation method of the delayed release bag of the repellent is the same as that in the example 1.

The use of the repellent sustained release bag and aggregation pheromone attractant core is the same as in example 1, and the other conditions are the same as in example 1. The results are shown in FIG. 4.

Comparative example 5

The ips typographus aggregation pheromone attractant core: the attractants are 2-methyl-3-buten-2 ol and S-cis-verbenol, and the preparation method and the using amount are the same as those in example 1, and the rest conditions are the same as those in example 1. The results are shown in FIG. 4.

In 7 months in 2019, the ips typographus aggregated pheromone attractant core and the repellent slow-release bag are placed in a forest, the distance between traps is 50m, the ips typographus aggregated pheromone attractant core and the repellent slow-release bag are hung between 2 trees and 100 centimeters above the ground, each treatment is repeated for 3 times, and the number of the ips typographus induced and caught by each treatment is the average number of 3 traps. According to the weather conditions, the number of bark beetles in the bark beetle collecting bottle is observed for 1 time every 2-3 days, and the collected bark beetles are placed in a plastic box and brought to a laboratory after counting, and the comparative example 4 is used as a control.

From the insect attraction data analysis in fig. 4 (average number of ips typographus per trap per observation) the results are: the repellent slow-release bag and the aggregation pheromone attractant attracting core are used simultaneously, only 3 ips typographus are captured on average in each observation, while the aggregation attractant attracting core is used alone to capture 107 ips typographus (comparative example 5), the inhibition rate reaches 97.2% (example 13), which indicates that nonanal and myrcene can be used as the repellent of ips typographus to simultaneously prevent and control ips typographus between forests or the combination of the nonanal and the myrcene and the aggregation pheromone attractant attracting core, thereby comprehensively preventing and controlling the harm of ips typographus.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The application of semiochemicals in repelling ips typographus is disclosed, wherein the semiochemicals comprise myrcene and/or nonanal.

2. A repellent sustained-release bag comprising the semiochemical of claim 1, absorbent cotton and polyethylene plastic bags.

3. The sustained-release bag for the repellent as claimed in claim 2, wherein the number ratio of the mass of the semiochemical to the polyethylene plastic bags is 1-16 g: 1.

4. The sustained-release bag for the repellent as claimed in claim 2, wherein the number ratio of the mass of the absorbent cotton to the polyethylene plastic bags is 2-3 g: 1.

5. The repellent sustained-release bag according to any one of claims 2 to 4, further comprising: antioxidants and ultraviolet light absorbers.

6. The repellent sustained release bag of claim 5, wherein the antioxidant comprises butyl hydroxyanisole or 2, 6-di-tert-butyl p-cresol and the UV absorber comprises 2-hydroxy-4-methoxybenzophenone.

7. The sustained-release bag for the repellent as claimed in claim 5, wherein the ratio of the mass of the antioxidant to the number of the polyethylene plastic bags is 5-10 mg: 1.

8. The sustained-release bag for the repellent as claimed in claim 5, wherein the ratio of the mass of the ultraviolet absorber to the number of the polyethylene plastic bags is 5-10 mg: 1.

9. The use of the repellent sustained-release bag of any one of claims 2 to 8 for repelling ips typographus.

10. The application according to claim 9, wherein the application comprises: and (3) hanging the repellent slow-release bags in the forest, wherein the number of hung repellent slow-release bags per mu is 5-8.