CN111587882B

CN111587882B - Sex attractant and attracting core for small-line angle bark beetle

Info

Publication number: CN111587882B
Application number: CN202010295671.0A
Authority: CN
Inventors: 宗世祥; 陶静; 张金桐; 骆有庆; 任利利; 黄建东
Original assignee: Beijing Changhui Linhai Ecological Technology Co ltd; Beijing Forestry University
Current assignee: Beijing Changhui Linhai Ecological Technology Co ltd; Beijing Forestry University
Priority date: 2020-04-15
Filing date: 2020-04-15
Publication date: 2021-10-12
Anticipated expiration: 2040-04-15
Also published as: CN111587882A

Abstract

The invention relates to the technical field of insect sex attractants, in particular to a sex attractant and a lure for a small-angle bark beetle. The sex attractant for the small linear bark beetle comprises active ingredients of the sex attractant, wherein the active ingredients of the sex attractant comprise cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenyl alcohol acetate. The sex attractant for the small linear angle pissodes radiata is used for insect condition prediction, direct trapping and killing by an attraction method or prevention and control of the small linear angle pissodes radiata by an interference mating method, is beneficial to protecting the natural enemies of the small linear angle pissodes radiata, is beneficial to removing harm, fully exerts the power of prevention and control of the small linear angle pissodes radiata, improves the effect of prevention and control of the small linear angle pissodes radiata, can avoid applying chemical and agricultural chemicals, reduces pollution, protects the environment, and has remarkable social benefit and ecological benefit.

Description

Sex attractant and attracting core for small-line angle bark beetle

Technical Field

The invention relates to the technical field of insect sex attractants, in particular to a sex attractant and a lure for a small-angle bark beetle.

Background

The small linear angle bark beetle Streltzoviella insularris (Staudinger) belongs to Lepidoptera (Lepidotera) bark beetle family (Cossidae), is an important stem borer in China, mainly uses larvae to damage white wax, gingko, Sophora japonica, elm, paper mulberry and the like, the number of single plant insect mouths can reach hundreds, and the single plant insect mouths occur in large areas in succession in multiple provinces and cities such as Beijing, Tianjin, Hebei, Jiangsu, Jiangxi, Hunan, Shanghai and the like.

Many insects develop and mature to emit chemical substances to attract and mate with foreign insects, and the chemical substances which communicate during the mating process of insects are called insect sex pheromones or sex pheromones. The trapping method of attracting insects with artificially synthesized sex pheromone or the like is a good method for efficiently and pollution-free pest control and pest occurrence monitoring.

The use of insect sex pheromones for pest control is a hot spot of comprehensive pest control measures in chemical ecology. According to researches, female pheromones of lepidoptera insects generally belong to aliphatic compounds, the chemical structural characteristics of the female pheromones of different insects are shown as different in carbon chain length, double bond number and position, terminal functional properties and the like, and the small difference of the characteristics can determine the specificity of insect species. For studying insect sex pheromone components including cis-3-tetradecenol acetate, trans-3-tetradecenol acetate, cis-3-trans-5-tetradecenol acetate, cis-5-dodecenyl acetate, cis-7-tetradecenol acetate, cis-9-tetradecenol acetate, trans-3-tetradecenol, cis-7-tetradecenol, etc., the chemical structure of the main component is 14-carbon straight chain enol or its acetate, although 1 or 2 kinds of different kinds of sex pheromone components are completely identical, however, the mixture ratio of species is completely different, such as hippophae rhamnoides moth sex attractant, artemisia desertorum moth sex attractant, salix mongolica sex attractant, elm bark moth sex attractant and the like, which have been successfully developed and widely applied, however, the method for effectively trapping the small linear angle bark moth has not been reported so far.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a sex attractant and an attracting core for the small moth-shaped moth. In the mating stage of the small linear angle codling moth, the lure core containing the sex attractant is hung on the side branch of the host, so that the male small linear angle codling moth can be effectively attracted to seek a mate, and the trapping and killing can be effectively carried out.

Specifically, the invention provides the following technical scheme:

the sex attractant comprises active ingredients of the sex attractant, wherein the active ingredients of the sex attractant comprise cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenyl alcohol acetate.

Preferably, in the above sex attractant, the mass ratio of cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenol acetate is 1.0-1.2: 0.6-0.8: 0.2. proved by verification, the mass ratio of cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenyl alcohol acetate is in the range, and a better attraction effect can be obtained.

Preferably, the above-mentioned sex attractant further comprises a non-polar solvent, and preferably, the non-polar solvent is selected from petroleum ether or n-heptane.

Preferably, in the above sex attractant, the mass ratio of the active ingredient of the sex attractant to the nonpolar solvent is 1: 40 to 50.

Preferably, the sex attractant further comprises an antioxidant, the antioxidant can prolong the active period of the sex attractant, and the antioxidant preferably accounts for 5-9% of the total mass of the sex attractant.

Preferably, in the above sex attractant, the antioxidant is 2, 6-tert-butyl-p-cresol or butyl hydroxy anisole.

The invention also provides a sex attractant core of the small-angle moth-shaped sex attractant, which comprises the sex attractant and a releaser for containing the sex attractant. The trapping core of the invention is put into an insect trap, and can trap the male small linear horned beetle in forests.

Preferably, in the lure core, the total mass of the sex attractant in the releaser is 40-50 mg.

Preferably, in the above lure core, the material of the releaser is selected from natural rubber, synthetic rubber, polyethylene, polyvinyl chloride or viscose material.

The invention has the following beneficial effects:

(1) monitoring the pest situation of the small linear angle codling moth: the sex attractant for the small linear angle moth provided by the invention can be used for insect condition prediction, so that the occurrence and growth situations of the small linear angle moth in the forest can be known timely and accurately, and a scientific basis is provided for timely and effective prevention and treatment. The sex attractant of the invention is used for detecting and predicting the insect situation, has high sensitivity, strong specificity, convenient use and low cost, and is suitable for general popularization;

(2) mass trapping: an adult occurrence curve is made according to the insect condition monitoring result, a trapper containing the sex attractant for the small trogopterus bark beetle provided by the invention is applied in the adult growth period, and male insects for mate mating are trapped and killed in time, so that the male insects lose mating opportunities and can not effectively propagate offspring, and harm is reduced;

(3) and (3) interfering mating: the natural attractant is generally applied in woodlands, so that the normal mating of male and female moths of the small linear horned beetles can be effectively interfered, the moths can not be effectively propagated for later generations, and the harm is reduced.

(4) The sex attractant can also be used in combination with a chemical infertility agent, virus or bacteria, and the sex attractant can lure male insects to contact with the infertility agent, virus or bacteria, and then fly away to contact and mate with other insects, thus causing much more damage to other populations than simply catching and killing the male insects.

Detailed Description

The present invention will be described in further detail with reference to specific examples, but the scope of the present invention is not limited thereto.

Example 1

A sex attractant for the bark beetle is composed of (by weight parts) sex attractant active component 1, nonpolar solvent 40, and antioxidant 4. Wherein the active ingredients of the sex attractant are cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenol acetate, and the mass ratio is 1.0:0.8: 0.2; the nonpolar solvent is petroleum ether, and the antioxidant is 2, 6-tert-butyl-p-cresol.

Comparative example 1

Comparative example 1 differs from example 1 only in that: the sex attractant comprises the effective components of cis-3-tetradecenol acetate and trans-3-tetradecenol acetate, and the mass ratio is 1.0: 0.1.

test example 1

Cleaning rubber stoppers with better slow release effect, airing, and respectively dripping the sex attractant solutions of the example 1 and the comparative example 1 into the concave parts of the rubber stoppers by using a micropipette according to the amount (50 microliter) set by experiments to prepare the lure cores; and then the trapping core is put into a triangular viscose trap, and the trapping core can be hung in the field for trapping tests.

The experiment is carried out from 7/8 days in 2018 to 8/12 days in 2018, 20 traps are hung on white wax trees on two sides of a northern road and a static road of a university of forestry of Haizu district, Beijing, 10 traps are hung on the white wax trees on two sides of a northern road and a static road of the university of Haizu district, example 1 and comparative example 1 respectively, the traps of different treatments are alternately placed, the hanging height of the traps is 2m, the distance between the traps is about 20m, the number of imagoes of the ips warrior beetles in the traps is investigated and counted every 7 days, and the total investigation is carried out for 5 times. The statistical results are shown in table 1.

TABLE 1 results of the trapping test

Test example 2

To further verify the trapping effect of the sex attractant of example 1, trapping tests were carried out in 3 sites set in the beijing foyer area and the tongzhou area from 18 days 6 and 18 days 2019 and 12 days 9 and 2019. 20 traps (see test example 1 for the production of traps) were installed at each test site, and were hung 2m from a white ash tree trunk at a trap pitch of about 20m, and the number of adult khaki moths in the traps was investigated and counted every 10 days for 7 times. Specific statistical data are shown in table 2.

TABLE 2 results of the trapping test

In conclusion, the sex attractant for the small-angle pissodes radiata is beneficial to protecting the natural enemies of the small-angle pissodes radiata, is beneficial to pest elimination, fully exerts the power of controlling the small-angle pissodes radiata, improves the effect of controlling the small-angle pissodes radiata, can avoid applying chemical and agricultural medicines, reduces pollution, protects the environment and has remarkable social and ecological benefits no matter being used for insect condition prediction, directly killing by an attraction method or preventing and controlling the small-angle pissodes radiata by an interference mating method.

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The sex attractant is characterized by comprising an active ingredient of the sex attractant, wherein the active ingredient of the sex attractant comprises cis-3-tetradecenol acetate, trans-3-tetradecenol acetate and cis-5-dodecenyl alcohol acetate; the mass ratio of cis-3-tetradecenol acetate to trans-3-tetradecenol acetate to cis-5-dodecenol acetate is (1.0-1.2): 0.6-0.8: 0.2.

2. the sex attractant of claim 1 further comprising a non-polar solvent.

3. The sex attractant of claim 2 wherein the non-polar solvent is selected from petroleum ether or n-heptane.

4. The sex attractant of claim 2, wherein the mass ratio of the sex attractant active ingredients to the non-polar solvent is 1: 40 to 50.

5. The sex attractant of any one of claims 1-4, further comprising an antioxidant.

6. The sex attractant of claim 5, wherein the antioxidant accounts for 5-9% of the total mass of the sex attractant.

7. The sex attractant of claim 6, wherein the antioxidant is 2, 6-tert-butyl-p-cresol or butyl hydroxyanisole.

8. A sex attractant core for a small moth of bark beetle, comprising the sex attractant of any one of claims 1 to 7 and a releaser for holding the sex attractant.

9. The sex attractant lure according to claim 8, wherein the total mass of the sex attractant in the releaser is 40-50 mg.

10. The sex attractant lure according to claim 8 or 9, wherein the releaser is made of a material selected from the group consisting of natural rubber, polyethylene, polyvinyl chloride, and viscose.