CN114304186A - Mandarin orange psylla trapping agent and application thereof - Google Patents
Mandarin orange psylla trapping agent and application thereof Download PDFInfo
- Publication number
- CN114304186A CN114304186A CN202111473383.0A CN202111473383A CN114304186A CN 114304186 A CN114304186 A CN 114304186A CN 202111473383 A CN202111473383 A CN 202111473383A CN 114304186 A CN114304186 A CN 114304186A
- Authority
- CN
- China
- Prior art keywords
- diaphorina citri
- trapping
- yellow
- trapping agent
- pests
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Catching Or Destruction (AREA)
Abstract
The invention discloses a diaphorina citri trapping agent and application thereof. The invention develops a diaphorina citri trapper and a method for trapping diaphorina citri pests on the basis that diaphorina citri female extract has trapping effect on diaphorina citri, namely, the invention provides a diaphorina citri physical and chemical trapping technology combining insect pheromone and color trapping technology, which can be used for preventing and controlling diaphorina citri pests. The invention utilizes the female diaphorina citri extract or methyl palmitate as a trapping agent, combines the yellow double-sided sticky trap to carry out green prevention and control on the diaphorina citri, is easy to operate and popularize, can obviously reduce the use amount of chemical pesticides in the prevention and control of diaphorina citri population, reduces the damage rate of rutaceae plants, protects the biodiversity and natural enemy resources, improves the prevention and control efficiency of the diaphorina citri, and opens up an important way for a comprehensive prevention and control technical system of the diaphorina citri pests.
Description
Technical Field
The invention belongs to the technical field of green prevention and control of agricultural pests. More particularly, relates to a diaphorina citri trapping agent and application thereof.
Background
Diaphorina citri (diaphorinaciti kuwayama) is a sucking insect of hemiptera, mainly harms rutaceae plants, and is the heaviest to damage citrus, namely wampee, murraya paniculata and citric acid. The diaphorina citri directly eats tender shoots of host plants, and the damaged tender shoots of the plants can wither, have new shoot distortion and the like; white honeydew is also secreted and adhered to branches and leaves during the development process of the diaphorina citri, so that the plants can suffer from soot diseases. More seriously, the diaphorina citri will carry the yellow dragon disease germs for the whole life after sucking the diseased plants infected with the yellow dragon disease of the citrus, and the virus transmission rate is very high. The diaphorina citri is fed, spawned and propagated on citrus yellow shoot diseased plants, a large amount of imagoes carrying yellow shoot pathogens can be generated, and the imagoes carrying yellow shoot pathogens can transmit yellow shoot pathogens (Huangglongbinging, HLB) by transferring new citrus plants, thereby bringing great threat to the healthy development of the citrus industry in China and even the world.
Although the method of agricultural control, physical control, biological control and chemical control are suggested in production to control the diaphorina citri, the chemical control is the main control method in the prior art. The long-term and large-scale use of chemical pesticides not only causes the death of a large number of natural enemy insects in orchards, the loss of biological diversity and the pollution to the ecological environment, but also causes a series of problems of pesticide residue of agricultural products exceeding the standard and the like, and simultaneously causes the drug resistance of diaphorina citri populations to different degrees. Therefore, the development of non-chemical control technology and products of diaphorina citri is urgently needed to develop green control.
The insect attractant or attractant means an active substance having a behavioral attraction effect on a specific insect, and includes insect pheromones, plant-derived information substances having an attraction effect on insects, artificially synthesized chemical substances, and the like. The chemical substances produced and released by insects for communication with each other are called insect pheromones, including sex pheromones, aggregation pheromones, and the like. Sex pheromones released by female adults can attract male adults, and the male adults find the female adults to mate and generate the next generation under the attraction of the sex pheromones. Therefore, sex pheromones are also often used as attractants for the integrated control of pests. Releasing sex pheromone of the female adult to attract the male adult in the field, and concentrating the attracted male adult into the trapping device to ensure that the male adult cannot mate with the female adult, namely reducing the number of the current pests and simultaneously reducing the next generation of pests.
Chinese patent 'a diaphorina citri attractant' discloses a diaphorina citri attractant based on volatile substances released by citrus, but the attractant contains more components and is relatively complex to prepare, and no report about diaphorina citri pheromone for controlling diaphorina citri is reported at present. In conclusion, the method finds and develops the diaphorina citri trapping agent with simple components and good trapping effect, and the comprehensive control technology for developing the diaphorina citri by utilizing the trapping agent has important significance for controlling the diaphorina citri and the citrus greening disease.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects and shortcomings of the prior art and provide a diaphorina citri trapping agent and application thereof.
The invention aims to provide a diaphorina citri trapping agent.
The second purpose of the invention is to provide the application of the trapping agent in trapping and controlling psyllid pests or preparing products for trapping and controlling psyllid pests.
The third purpose of the invention is to provide the application of the trapping agent in preventing and controlling citrus greening disease or preparing products for preventing and controlling citrus greening disease.
The fourth purpose of the invention is to provide a diaphorina citri trapper.
A fifth object of the present invention is to provide a method for controlling psyllium pests.
The above purpose of the invention is realized by the following technical scheme:
according to the method, after the n-hexane is used for soaking and extracting the female diaphorina citri, the selective behavior reaction of the adult diaphorina citri is measured, the extraction of the female diaphorina citri is found to have an trapping effect on the diaphorina citri, and the main components of the extraction are methyl palmitate, nonacosane-1-ene, 2-methyl heptacosane, n-nonacosane and 1-chlorodocosane through identification. On the basis that the female diaphorina citri extract has trapping effect on diaphorina citri, the invention develops a diaphorina citri trap and a trapping method of psylla pests. The invention utilizes the female diaphorina citri extract or methyl palmitate as a trapping agent and combines a yellow double-sided sticky trap to carry out green prevention, control and control on the diaphorina citri, namely, the invention provides a physical and chemical trapping technology of the diaphorina citri by combining insect pheromone and color trapping technology. The technology is simple, easy to operate and popularize, can obviously reduce the use amount of chemical pesticides in the control of the diaphorina citri populations, reduces the damage rate of citrus crops, protects biodiversity and natural enemy resources, improves the control efficiency of the diaphorina citri, and is environment-friendly and safe.
The invention firstly provides application of the diaphorina citri female extract in diaphorina citri trapping or preparation of a diaphorina citri trapping agent.
Preferably, the extract of the diaphorina citri female is an n-hexane soaked extract of the diaphorina citri female, see example 1.
The invention also provides application of any one or more of methyl palmitate, nonacosane-1-ene, 2-methyl heptacosane, n-nonacosane or 1-chlorodocosane in diaphorina citri trapping or preparation of diaphorina citri trapping agent.
The invention also provides a diaphorina citri trapping agent, which contains methyl palmitate.
Specifically, the trapping agent also contains nonacosane-1-ene, 2-methyl heptacosane, n-nonacosane and 1-chlorodocosane.
Specifically, the trapping agent is an extract of the diaphorina citri female insects.
The invention also provides an extraction method of the diaphorina citri female extract, which comprises the steps of soaking diaphorina citri female imagoes in normal hexane, continuously shaking the soaked solution for 30-40 min, taking supernatant and concentrating the supernatant by using nitrogen.
The trapping agent has a good trapping effect on the diaphorina citri, and the trapping agent is derived from diaphorina citri pheromone, so that the trapping agent also has a certain trapping effect on the diaphorina citri pests with close relationship with the diaphorina citri. Therefore, the application of the invention protects the application of the trapping agent in trapping and controlling psyllid pests or preparing products for trapping and controlling psyllid pests.
Preferably, the psyllid pest is a diaphorina citri, see example 1.
Since diaphorina citri can spread the citrus greening disease, controlling the quantity of diaphorina citri is helpful for controlling the citrus greening disease. Therefore, the invention also applies to protect the application of the trapping agent in preventing and controlling the citrus greening disease or preparing products for preventing and controlling the citrus greening disease.
The invention also provides a diaphorina citri trap which comprises a yellow double-sided sticky trap with an adhesive, wherein a trap core made of a trapping agent is arranged on the sticky trap.
Specifically, the trapping agent is methyl palmitate or a diaphorina citri female extract.
Specifically, the lure core is a rubber lure core.
Specifically, the invention injects trapping agent into the rubber head to prepare the rubber trapping core, and then fixes the prepared trapping core in the center of the yellow plate by using a nickel-plated pin to form the trap.
Specifically, when the rubber lure is manufactured, the rubber head is rinsed and soaked for 24-36 hours by using an organic solvent n-hexane, and then a trapping agent (methyl palmitate or an extract of the diaphorina citri) is injected into the rubber head to manufacture the rubber lure; the rubber lure core is fixed in the center of the yellow double-sided sticky trap after being placed in a room temperature ventilation place for volatilization for 24 hours before use.
Specifically, the concentration of the trapping agent is 0.1-100 mu g/mL.
Preferably, the concentration of the decoy is 100. mu.g/mL, see example 1 or 2.
Specifically, the yellow double-sided sticky trap is 20cm long and 15cm wide, and the wave band is 560-600 nm.
Preferably, the wavelength of the yellow double-sided sticky insect plate is 560nm, see example 2.
Specifically, the surface of the yellow double-sided insect sticking plate is provided with an adhesive, and the adhesive is one of an adhesive, engine oil, peanut oil or vaseline.
Preferably, the adhesive is an adhesive, see example 3.
Preferably, the thickness of the adhesive is 0.5mm, see example 3.
The invention also applies to protect the application of the trap in trapping and controlling the wood louse pests or the citrus greening disease.
The invention also provides a method for controlling the wood louse pests, namely the trap is hung on the host plants of the wood louse pests.
The hanging heights of the trap are three, namely the top, the upper part and the middle part. Specifically, the top is that the bottom of the yellow double-sided sticky trap in the trap is 30cm higher than the top of the host plant; the upper part is that the top of the yellow double-sided sticky trap is flush with the top of the host plant; the middle part is that the top of the yellow double-sided sticky trap is flush with the middle part of the host plant.
Preferably, the height of the suspension of the trap is upper, see example 3.
The invention also applies to protect the application of the method in trapping and controlling psyllid pests or preparing products for trapping and controlling psyllid pests.
The invention also provides application of the method in preventing and treating citrus greening disease or preparing products for preventing and treating citrus greening disease.
The invention has the following beneficial effects:
the research result of the invention shows that the female diaphorina citri extract has trapping effect on diaphorina citri, and can be used for trapping and controlling diaphorina citri pests and controlling citrus yellow dragon disease. On the basis of the trapping effect of the diaphorina citri female extract on the diaphorina citri, the invention develops a diaphorina citri trap and a trapping and control method of psylla pests. The trap and the control method are simple, easy to operate and popularize, can remarkably reduce the use amount of chemical pesticides in the control of diaphorina citri populations, reduce the damage rate of rutaceae plants, have good control effect, are environment-friendly and safe, cannot induce the drug resistance of pests, cannot damage the biodiversity and protect the biodiversity and natural enemy resources.
In addition, the invention can further promote the combination of insect pheromone and other control means to achieve the purpose of efficiently controlling the population quantity of the pests and open up an important way for the comprehensive control technology system of the psyllid pests.
Drawings
FIG. 1 shows the results of the selective behavioral responses of adult diaphorina citri males and females to diaphorina citri extracts, respectively, with P < 0.01.
Fig. 2 is a total ion flow graph of the constituents of a diaphorina citri female extract.
FIG. 3 shows the behavioral response of the males of diaphorina citri to different concentrations of methyl palmitate, representing P < 0.01.
FIG. 4 shows the effect of the extracts of yellow plates and female adults on the total amount of diaphorina citri induced.
FIG. 5 shows the effect of yellow plates and methyl palmitate on the total amount of diaphorina citri induced.
Detailed Description
The invention is further described with reference to the drawings and the following detailed description, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
The host plant used in the present invention is murraya paniculata (murrayapaniculata jacks); the source of the test insects was diaphorinacri (Diaphorinacitri).
The test equipment used by the invention comprises an insect stereoscope (Motic K-400L), an insect suction pipe, an insect receiving net (25 cm in length and 20cm in width), a small finger-shaped pipe (3 cm in length and 0.5cm in diameter), "Y" -shaped olfactometer, a yellow double-sided sticky insect plate (20 cm in length and 15cm in width and provided with an adhesive on the surface, and good multi-trade company Limited responsibility company), a rubber head (Beijing Zhongji tetragonal Biotechnology corporation), a 10mL centrifuge tube, double-circle qualitative filter paper (Hangzhou Wahua filter paper Limited company) and a nickel-plated pin (Shanghai Olympic culture article Limited company).
Example 1 extraction and assay analysis of diaphorina citri mimicry information Compounds
1. Extraction of diaphorina citri informational compounds
After separating the male and female diaphorina citri by using insect sight glass, extracting the extract of the adult diaphorina citri female and male by a solvent extraction method respectively, wherein the extract contains diaphorina citri information compounds.
Putting 50 diaphorina citri of the same sex into a 10mL centrifuge tube, injecting 500 mu L n-hexane (chromatographic purity) to soak for 30min, shaking the centrifuge tube by hands continuously during the process, transferring the supernatant into a 2mL centrifuge tube by using a liquid transfer gun, and concentrating to 50 mu L by using nitrogen to obtain the diaphorina citri imago extract. The extract is sealed and then stored in a refrigerator at low temperature (-20 ℃) so as to be used for later behavior determination and field test.
2. Selectivity behavior determination of diaphorina citri on mimotope semiochemicals
Taking an extraction solvent n-hexane as a contrast, and respectively measuring the behavior reaction of the adult diaphorina citri males and females to the extract of the diaphorina citri and the male insect by using a Y-type olfactometer indoors.
The results of the selective behavior response of the adult diaphorina citri males and females to the diaphorina citri female and male extracts, respectively, are shown in fig. 1. As can be seen from FIG. 1, the numbers of both the male and female diaphorina citri did not reach significant levels (X;) in the treatment of the male insect extract and the n-hexane solution2＝1.25，df＝1，P＝0.26；χ21.74, P0.19). In the treatment of female insect extract and n-hexane solution, the difference of the female diaphorina citri quantity of the female insect extract and the n-hexane solution is not significant (X)20.51, df 1, P0.48). The quantity of the diaphorina citri selected from the female insect extract is obviously more than that of the diaphorina citri (chi) selected from the n-hexane solution2＝14.74，df＝1，P<0.001). The results show that the diaphorina citri female and male extract has obvious influence on the selection behavior of the diaphorina citri adults, and the diaphorina citri female extract has obvious trapping effect on the diaphorina citri males. The induced male worms can break the sex ratio of female male worms and reduce the mating probability of the diaphorina citri populations, thereby achieving the purpose of reducing the number of the diaphorina citri populations.
3. Identification and analysis of diaphorina citri informational compound GC-MS
In the result of the selective behavior determination, the trapping effect of the diaphorina citri female insects is most obvious, so the GC-MS is utilized to analyze the components of the diaphorina citri female insect extract.
The extract was analysed on an Agilent 6890N-5973N GC-MS column (30 m.times.0.25 mm. times.0.25 mm) HP-5MS capillary column. The method comprises the following specific steps: the sample was injected 2 μ L each time without split flow manually, with an injection port temperature of 250 ℃. The temperature rising procedure is as follows: keeping at 80 deg.C for 1min, heating to 280 deg.C at a speed of 10 deg.C/min, and keeping for 20 min. The carrier gas was helium at a flow rate of 1 mL/min. Ionization mode EI, ionization energy is 70 eV. The temperature of the ion source emitter is 230 ℃, and the mass scanning range is 30-600 amu.
The invention obtains the total ion flow diagram of the female diaphorina citri extract by performing GC-MS analysis on the female diaphorina citri extract (as shown in figure 2). 5 compounds with relatively high content were identified and screened from the diaphorina citri female extract by NIST mass spectrometry system search (as shown in table 1).
TABLE 1 GC-MS identification of the extract of the female diaphorina citri
4. Determination of selection behavior of diaphorina citri male adults on methyl palmitate
Because only a methyl palmitate standard is available on the market, the selective behavior reaction of the diaphorina citri male insects to the methyl palmitate is only measured. Specifically, methyl palmitate (purity > 95%) is dissolved in n-hexane solution, concentration gradients are set to be 1, 10 and 100 mu g/mL, behavior selection reactions of 3 different concentrations of methyl palmitate by the male and female diaphorina citri are measured by a Y-type olfactometer indoors, and the male and female diaphorina citri are repeated 10 times respectively.
The behavioral response results of the diaphorina citri males to different concentrations of methyl palmitate are shown in figure 3. As can be seen from FIG. 3, the number of insects selected by the male diaphorina citri did not reach a significant level (chi) in the treatment of methyl palmitate and n-hexane solutions at concentrations of 1. mu.g/mL and 10. mu.g/mL2 10μg＝0.01，df＝1，P＝0.920；χ2 10μg0.67, df 1, P0.414). And in the treatment room of methyl palmitate and n-hexane solution with the concentration of 100 mu g/mL, the number of selected insects of the male diaphorina citri is obviously higher than that of the n-hexane solution (chi)2 10μg＝8.67，df＝1，P＝0.003；)。
Example 2 Effect of yellow plates and mimotope on the trapping Effect of diaphorina citri
1. Influence of yellow board, female insect and male insect extracts on total amount of diaphorina citri induced collection
The test was carried out in a screen chamber, which was separated into two zones (divided into treatment A zone and treatment B zone) by a gauze. Before the test is started, the diaphorina citri population densities of the plants in the two treatment areas of the net room A, B are observed and adjusted respectively, so that the diaphorina citri population densities of the plants in the areas are approximately the same.
Preparing a lure core and a blank lure core: the method of example 1 is used for extracting the extract of the female and male adult diaphorina citri as a trapping agent. When the lure core is manufactured, the rubber head is rinsed for 24-36 hours by organic solvent n-hexane, and then the lure agent is injected into the rubber head to manufacture the lure core. Adding 100 μ L of the extract into rubber lure (produced by Beijing Zhongjie Sifang Biotech Co., Ltd.) to obtain lure, volatilizing at room temperature and ventilation for 24 hr before use, and volatilizing the extraction solvent to obtain the lure. 100 mu L of n-hexane solution is added into the rubber head to be used as a blank lure.
According to the invention, according to the classical chromatogram compiled by the compiling and publishing committee of the Chinese academy of sciences, a yellow plate (with the wave band of 560-600 nm) is preferably selected as the efficient diaphorina citri color induction device through tests, and the optimal wavelength of the effect in the tests is 560 nm.
Preparing a diaphorina citri trapper: selecting a yellow double-sided sticky trap (with wavelength of 600nm) with size of 20cm × 15cm, and then simply called yellow plate, fixing the lure core and the blank lure core at the center of the yellow plate by using a nickel-plated pin to obtain the trap, and suspending the trap between the Murraya koenigii plants in the A, B treatment area.
The treatment zone a trial had three treatments: (1) yellow board and male insect attracting core; (2) yellow board and blank lure core; (3) yellow board;
the treatment zone B test was designed with three treatments: (1) yellow board and female insect attracting core; (2) yellow board and blank lure core; (3) and (5) yellow board.
During suspension, the distance between every trap (yellow board) is 3m, the traps are randomly arranged in a row, the suspension heights of the yellow boards are all kept consistent, 12 yellow boards are suspended in a single area, and each treatment is repeated for 3 times. Taking off the yellow board 3 days later and respectively recording the number and total amount of the induced female and male diaphorina citri; after the end of the recording, a new yellow plate was replaced and the test continued for a total of 15 days.
The effect of the extracts of yellow plates and female adults on the total amount of diaphorina citri induced collection is shown in fig. 4. As can be seen from fig. 4, in the three treatments in field B treatment zone, the diaphorina citri and female extract combined trapping male ratio was significantly higher than in the remaining two treatments, up to 66.28%, consistent with the diaphorina citri versus female extract selection behavior reaction results; while the induced female rate of diaphorina citri was significantly lower than the remaining two treatments, only 33.72%. Compared with the single yellow board treatment, the yellow board and the n-hexane combined treatment have the advantages that the difference of the induced and collected diaphorina citri male rate is not significant and is respectively 48.18 percent and 46.74 percent, and the difference of the induced and collected diaphorina citri female rate between the two treatments is not significant and is respectively 51.82 percent and 53.26 percent. Although the female ratio of the combined induced collection of the yellow board and the female extract is obviously lower than that of the other two treatments, the male ratio of the combined induced collection of the yellow board and the female extract is obviously higher than that of the other two treatments, and the induced collected males can break the sex ratio of the female males in an orchard and reduce the mating probability of the diaphorina citri population, so that the aim of reducing the quantity of the diaphorina citri population is fulfilled.
2. Influence of yellow plates and methyl palmitate with different concentrations on diaphorina citri trapping effect
The test was conducted in a screen room, which was partitioned into three areas (treatment A, treatment B and treatment C, respectively) by a gauze. The diaphorina citri population densities of plants in the three treatment zones of the net room A, B, C were observed and adjusted before the start of the test to ensure that the diaphorina citri population densities were approximately the same among the plants in the zones.
Preparing a lure core and a blank lure core: n-hexane is used as a solvent, methyl palmitate (1, 10 and 100 mu g/mL) solutions with different concentrations are respectively prepared, 100 mu L of the solution is respectively taken, the lure and the blank lure are prepared by the same method as that in the step 1, and the lure with methyl palmitate concentrations of 1, 10 and 100 mu g/mL is obtained.
Selecting a yellow board with the size of 20cm multiplied by 15cm, hanging the yellow board among the murraya paniculata plants in A, B, C three treatment areas, and fixing the lure and the blank lure at the center of the yellow board by using a nickel-plated pin. The treatment zone a trial had three treatments: (1) yellow plate and 1 microgram/mL methyl palmitate lure core; (2) yellow board and blank lure core; (3) and (5) yellow board. The treatment zone B test was designed with three treatments: (1) yellow plate and 10 mug/mL methyl palmitate lure; (2) yellow board and blank lure core; (3) and (5) yellow board. Treatment zone C test three treatments were set: (1) yellow plate and 100 mug/mL methyl palmitate lure; (2) yellow board and blank lure core; (3) and (5) yellow board. The distance between every two yellow boards is 3m, the yellow boards are randomly arranged in a row, the hanging heights of the yellow boards are all kept consistent, 12 yellow boards are hung in a single area, and each treatment is repeated for 3 times. And (3) hanging the plates, taking down the yellow plates, recording the number and the total amount of the induced female and male diaphorina citri after 3 days, replacing the yellow plates with new yellow plates, and continuing the test for 15 days.
The effect of yellow plates and methyl palmitate on the total amount of adult diaphorina citri induced are shown in fig. 5. As can be seen from figure 5, in the three treatments in field C treated area, the ratio of males induced by yellow panels in combination with 100 μ g/mL methyl palmitate was significantly higher than the remaining two treatments, up to 67.01%, while the ratio of females was significantly lower than the remaining two treatments, only 32.99%.
The total amount of diaphorina citri induced in the field C treated areas by the different treatment combinations of the yellow plates is shown in table 2. From the analysis in table 2, in the three treatments in the field C treatment area, the total amount of the diaphorina citri induced by the combination of methyl palmitate and yellow board of 100 μ g/mL is more, the average is 18.60 heads/sheet, the total amount of the diaphorina citri induced by the combination of yellow board and n-hexane is 17.20 heads/sheet, which is slightly lower than the induced amount of the yellow board of 17.67 heads/sheet, but the total amount of the diaphorina citri induced by the three treatments is not significant (F is 0.23; df is 2, 42; P is 0.797).
TABLE 2 Total amount of diaphorina citri induced by different combinations of treatment with yellow plates in field C treatment area
Note: the data in the table are mean values +/-standard errors, the same letter in the same row represents that the difference of the number of induced diaphorina citri is not significant among different treatments, and the significance level is 0.05.
The results show that the methyl palmitate and the yellow board are used together, so that the remarkable control effect on the diaphorina citri can be achieved in a short time, and the trapping, trapping and killing effects on the diaphorina citri are remarkably higher than those of the normal hexane, the yellow board and the single yellow board. Therefore, the methyl palmitate and the yellow board can be used together during prevention and treatment, the prevention and control effect of the diaphorina citri can be effectively and obviously improved, the prevention and control effect is stable, the environment is safe, the biodiversity cannot be damaged, and the diaphorina citri cannot generate drug resistance.
Example 3 Effect of yellow Panel hanging and adhesive type on the Pediculus citri trapping Effect
1. Influence of different suspension heights of yellow board on trapping diaphorina citri
Yellow plates with the size of 20cm multiplied by 15cm are selected and hung among the plants in the treatment area A, and the hanging modes are three: (1) the bottom of the yellow plate is 30cm higher than the top of the murraya paniculata plant (collectively referred to as top); (2) the top of the yellow board is flush with the top of the murraya paniculata plant (collectively referred to as the upper part); (3) the top of the yellow plate is flush with the middle part of the murraya paniculata plant (collectively called the middle part). The distance between every two yellow boards is 3m, the line spacing is 1m, the yellow boards with each hanging height are repeated for 3 times and are randomly arranged in a row, and 9 yellow boards are hung in total. And (3) taking off the yellow board after the board is hung for 6 days, recording the number of the induced psyllids, and then changing a new yellow board, wherein the hanging board is hung for 5 times every month.
The amount of diaphorina citri induced by three different hanging heights is shown in table 3. As can be seen from Table 3, the difference in the amount of the diaphorina citri induced by different hanging heights of the yellow board reaches a significant level (P < 0.05). The test survey results in different months show that the trapping amount of the upper suspension yellow board to the diaphorina citri is the largest, the average trapping amount reaches 92.96 heads/sheet, and the difference of the trapping amount with the height of the rest 2 suspension yellow boards reaches a significant level (F is 21.65, df is 2,132, and P is less than 0.001); the trapping number of the height of the yellow board hung on the top is several times, and the average number reaches 59.20 heads/sheet; the trapping number is the least with the height of the middle suspension yellow board, and the average is 56.44 heads/piece. The difference of the trapping amount of diaphorina citri between the top and middle suspended yellow plates was not significant (P > 0.05).
TABLE 3 number of diaphorina citri induced at different hanging heights
Note: the data in the table are mean values +/-standard errors, and different letters in the same row represent that the differences of the quantity of the induced citrus psyllids collected at different hanging heights of the yellow board are obvious, and the significance level is 0.05.
2. Effect of different adhesive types on yellow board surface on trapping diaphorina citri
Yellow boards (with adhesive on the surface) and yellow plywood with the size of 20cm multiplied by 15cm are selected. The surfaces of the yellow three-ply boards are respectively treated by different adhesives, and peanut oil, engine oil and vaseline are respectively and uniformly coated on the surfaces of the three-ply boards, wherein the thickness of the three-ply boards is 0.5 mm. Suspending yellow boards and treated yellow plywood between plants in the treatment area C, wherein the distance between the boards is 3m, the boards are randomly arranged in a row, the suspension height is consistent, each treatment is repeated for 3 times, and 12 boards are suspended in total. The plate is hung 6 days later, the plate is taken out, the number of the induced psyllids is recorded, then a new plate is replaced, and the plate is hung 5 times per month.
The amounts of 4 different adhesive types induced diaphorina citri are shown in table 4. As can be seen from table 4, the difference in the amount of the 4 different adhesive types on the yellow panel surface induced the diaphorina citri to a significant level (P <0.05), with the adhesive being optimal.
TABLE 4 yellow panel shows the amount of diaphorina citri induced by different adhesive types
Note: the data in the table are mean values ± sem, and different letters in the same row represent yellow plates, indicating that the differences in the number of diaphorina citri induced by different adhesive types were significant, with a significance level of 0.05.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The diaphorina citri trapping agent is characterized by containing methyl palmitate.
2. The trapping agent according to claim 1, wherein the trapping agent further comprises nonacosane-1-ene, 2-methyl heptacosane, n-nonacosane and 1-chlorodocosane.
3. The trapping agent according to claim 2, wherein the trapping agent is a diaphorina citri female extract.
4. The use of the trapping agent of any one of claims 1 to 3 in trapping and controlling psyllid pests or in preparing products for trapping and controlling psyllid pests.
5. Use according to claim 4, wherein the psyllid pest is diaphorina citri.
6. The application of the trapping agent as claimed in any one of claims 1 to 3 in preventing and treating citrus greening disease or preparing products for preventing and treating citrus greening disease.
7. A diaphorina citri trap, which is characterized by comprising a yellow double-sided sticky trap with a binder, wherein a trap core made of the trapping agent disclosed by any one of claims 1-3 is arranged on the sticky trap.
8. A trap according to claim 7, characterized in that the concentration of the trapping agent in the rubber trapping core is 0.1-100 μ g/mL, and the wave band of the yellow double-sided sticky trap is 560-600 nm.
9. Use of the trap according to claim 7 or 8 for trapping, controlling psyllid pests or controlling citrus greening disease.
10. A method of controlling wood louse pests, characterised in that a trap according to claim 7 or 8 is suspended from a host plant of wood louse pests.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111473383.0A CN114304186A (en) | 2021-12-02 | 2021-12-02 | Mandarin orange psylla trapping agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111473383.0A CN114304186A (en) | 2021-12-02 | 2021-12-02 | Mandarin orange psylla trapping agent and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114304186A true CN114304186A (en) | 2022-04-12 |
Family
ID=81048490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111473383.0A Pending CN114304186A (en) | 2021-12-02 | 2021-12-02 | Mandarin orange psylla trapping agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114304186A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116098136A (en) * | 2023-03-09 | 2023-05-12 | 河北省农林科学院植物保护研究所 | Method for attracting pear psyllids in pear garden by combining different-color insect attracting plates |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477157A (en) * | 2018-05-22 | 2018-09-04 | 华中农业大学 | A kind of diaphorina citri attractant |
| US20190059358A1 (en) * | 2016-09-13 | 2019-02-28 | Guangdong Institute Of Applied Biological Resources | Use of pyemotes zhonghuajia for preparing biological control agents of diaphorina citri |
| CN110122487A (en) * | 2019-05-23 | 2019-08-16 | 赣南师范大学 | Alcohol is luring application and a kind of diaphorina citri attractant in diaphorina citri |
-
2021
- 2021-12-02 CN CN202111473383.0A patent/CN114304186A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190059358A1 (en) * | 2016-09-13 | 2019-02-28 | Guangdong Institute Of Applied Biological Resources | Use of pyemotes zhonghuajia for preparing biological control agents of diaphorina citri |
| CN108477157A (en) * | 2018-05-22 | 2018-09-04 | 华中农业大学 | A kind of diaphorina citri attractant |
| CN110122487A (en) * | 2019-05-23 | 2019-08-16 | 赣南师范大学 | Alcohol is luring application and a kind of diaphorina citri attractant in diaphorina citri |
Non-Patent Citations (2)
| Title |
|---|
| 林妗蓓 等: "７种挥发性化合物对柑橘木虱引诱效果的评价", 《植物保护》 * |
| 许炜明: "黄板与拟性信息化合物对柑橘木虱的诱集反应及其应用", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116098136A (en) * | 2023-03-09 | 2023-05-12 | 河北省农林科学院植物保护研究所 | Method for attracting pear psyllids in pear garden by combining different-color insect attracting plates |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hussain et al. | Behavioral and electrophysiological responses of Coptotermes formosanus Shiraki towards entomopathogenic fungal volatiles | |
| Dardouri et al. | Repellence of Myzus persicae (Sulzer): evidence of two modes of action of volatiles from selected living aromatic plants | |
| Piesik et al. | Behavioural responses of wheat stem sawflies to wheat volatiles. | |
| CN1269400C (en) | Tea garden insect trapping method | |
| Piesik et al. | Volatile induction of three cereals: influence of mechanical injury and insect herbivory on injured plants and neighbouring uninjured plants | |
| CN111328604A (en) | Green prevention and control method for diaphorina citri | |
| Niu et al. | A push–pull strategy for controlling the tea green leafhopper (Empoasca flavescens F.) using semiochemicals from Tagetes erecta and Flemingia macrophylla | |
| CN113068695B (en) | Sex attractant for oriental fruit moth, lure and preparation method and application thereof | |
| CN110800743A (en) | Preparation method and application technology of pheromone of spotted ceragus lucorum and attractant lure of pheromone | |
| Liu et al. | Volatile emission in wheat and parasitism by Aphidius avenae after exogenous application of salivary enzymes of Sitobion avenae | |
| CN107396927B (en) | Adult drosophila ziziphus jujuba attractant and application thereof | |
| CN114304186A (en) | Mandarin orange psylla trapping agent and application thereof | |
| Munawar et al. | Heat stress affects potato's volatile emissions that mediate agronomically important trophic interactions | |
| CN107535460B (en) | Insect attracting plate and preparation method thereof | |
| CN114698638B (en) | Application of caproic acid and caproic acid hexyl ester in repelling solenopsis invicta | |
| CN113243367A (en) | Efficient composite insect sex attractant for attracting prodenia litura and oriental tobacco budworms | |
| CN111165490B (en) | Application of information chemical substance in repelling ips typographus and repellent slow-release bag and application thereof | |
| CN111587882B (en) | Sex attractant and attracting core for small-line angle bark beetle | |
| CN114617125B (en) | Application of 2-methoxy-3-methyl-1, 4-benzoquinone in prevention and treatment of solenopsis invicta and Monochamus alternatus | |
| CN110720460B (en) | Plant source pollution-free active substance for attracting green gram, attractant and method | |
| CN110786326B (en) | Application of 2-ethylhexanoic acid | |
| CN110742072A (en) | Pest attractant and preparation method and application thereof | |
| CN112042647A (en) | Attractant for adult anoplophora chinensis | |
| KUMAR et al. | Herbivore induced plant volatiles represents a favorable host to onion thrips (Thrips tabaci) | |
| CN117397681B (en) | Barbary wolfberry fruit sex pheromone and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |