CN114698638B - Application of caproic acid and caproic acid hexyl ester in repelling solenopsis invicta - Google Patents
Application of caproic acid and caproic acid hexyl ester in repelling solenopsis invicta Download PDFInfo
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- CN114698638B CN114698638B CN202210571999.XA CN202210571999A CN114698638B CN 114698638 B CN114698638 B CN 114698638B CN 202210571999 A CN202210571999 A CN 202210571999A CN 114698638 B CN114698638 B CN 114698638B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/14—Insects
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses application of caproic acid and hexyl caproate in repelling solenopsis invicta. The caproic acid and the hexyl caproate which are screened and developed from the compounds for defending against the chest odor gland of the adelphocoris suturalis have strong repelling effect on the ergates of the solenopsis invicta, and can be used for developing behavior repellents of the solenopsis invicta and other ants and quarantine application; compared with other chemorepellents aiming at the solenopsis invicta at the present stage, the chemorepellent utilizes the defensive substances naturally generated by insects, is more green and environment-friendly, and has important market application and popularization values.
Description
Technical Field
The invention belongs to the technical field of green prevention and control of solenopsis invicta, and particularly relates to application of caproic acid and hexyl caproate in repelling solenopsis invicta.
Background
During the long-term co-evolution of insects in nature with their natural enemies and niche competitors, various resistance mechanisms including physical, chemical and behavioral defenses are developed to repel predators of natural enemies and compete with other kinds of insects. The chemical defense is that insects repel natural enemies and interfere with competitors' behaviors by using chemical substances which are widely available and can be directly derived from host plants eaten by the insects, glandular secretion or endophytic bacteria and the like. Therefore, chemical defense substances of insects have extremely important utilization values in the aspects of developing biological repellents of pests and the like. Stinkbugs (stinkbugs) insects are widely distributed and very abundant in species, are distinguished by notorious chemobehavior defense characteristics, and are ideal materials for researching insect chemodefense and repellent development. When the insect pest is attacked by surprise or natural enemies (ants, birds and frogs), the stinkbugs can quickly secrete and release chemical defense substances with repelling/toxicity outwards, and the chemical defense substances synthesized by the insect pests have important utilization values in the aspects of developing pest repellents, biological insecticidal pesticides and the like.
Disclosure of Invention
The invention aims to provide a natural compound medicament with strong repelling effect on red fire ants and application thereof, aiming at the problems that the red fire ants are rapidly diffused and spread and increasingly rampant in the prior art, and the repellent medicaments of the red fire ants are not of a plurality of types and have high toxicity to people and livestock in the prior quarantine monitoring technology.
According to the method, through experimental technologies such as gas chromatography-mass spectrometry (GC-MS), gas chromatography-antennal potential combination (GC-EAD), ethovisionXT behavior trajectory tracking and the like, the compounds n-hexanoic acid and hexyl hexanoate which are secreted by the chest stinkbug based on the adelphocoris rudis are obtained through screening, and the fact that the n-hexanoic acid and hexyl hexanoate have a high-efficiency repelling effect on the imported fire ants is proved.
Accordingly, it is a first object of the present invention to provide the use of hexanoic acid and hexyl hexanoate for the repellency of agroforestrial pests.
Preferably, the agricultural and forestry pests are ant pests.
Preferably, the ant pest is a red imported fire ant.
Preferably, the solenopsis invicta is a solenopsis invicta worker ant.
An agroforestry pest repellant comprising an effective amount of hexanoic acid and/or hexanoic acid hexyl ester, and a carrier.
Preferably, the agricultural and forestry pest repellent agent is a red imported fire ant repellent agent.
A method for repelling Solenopsis invicta comprises applying caproic acid and/or hexyl caproate to the area to be repelled, and repelling Solenopsis invicta with caproic acid and/or hexyl caproate.
Preferably, the application mode can be spraying, pouring and the like.
The caproic acid and hexyl caproate screened and developed from the hind chest stinky gland defensive substances of the dolphin largehead have good repellent effect on the solenopsis invicta. Therefore, the repellent and the repelling method for preventing and controlling the invasive pest Solenopsis invicta can be used for development and quarantine application of behavior repellents of Solenopsis invicta and other ants, and compared with chemical repellent agents for Solenopsis invicta at the present stage, the repellent and the method utilize defensive substances naturally generated by insects, are more green, safe and environment-friendly, and have important market application and popularization potential.
Drawings
FIG. 1 shows a large leg stinkbugAnoplocnemis castaneaAnd a schematic representation of the location of the posterior skyhook gland.
Figure 2 is a GC-MS total ion flow graph of the retro-tonoplast compound of rhus giganteus.
FIG. 3 is a graph of the antennal GC-EAD response of red imported fire ants to the retro-tonoplast compound of adelphocoris courbarii.
FIG. 4 is a graph of the repellent effect of a retro-tonoplast compound on red imported fire ants; wherein, (a) a schematic experimental design; (b) repellent results.
FIG. 5 is a trace plot of the behavior of red fire ants in the area of the tonoplast compound treatment experiment after the adelphocoris rudis; wherein (a) hexanoic acid; (b) hexyl acetate; (c) hexyl butyrate; (d) hexyl caproate.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1: GC-MS separation and identification of compounds of retrosternal stinkbug of crinis rudinifolius
S1 extraction of glandular compound component by solution leaching method
The adult stinkbug with thick leg is stimulated by using sharp tweezers, after the adult stinkbug secretes yellow chemical defense substances from the retrothoracic gland (figure 1), the yellow secretion is absorbed by a capillary tube, and the liquid secretion absorbed by the capillary tube is dissolved in a sample bottle containing 1 mL of chromatographically pure hexane. Then adding a small amount of anhydrous Na 2 SO 4 Shaking with shaking for 2 min to remove excess water from the sample, filtering the solution through an organic filter of 0.45 μm pore size, followed by concentration to 100 μ L with nitrogen. The concentrated samples were stored in a-20 ℃ freezer for future use.
S2 identification of Compounds
The samples prepared in S1 were identified by gas chromatography-mass spectrometer (model: Agilent 8890-:
(1) chromatographic conditions are as follows: the temperature of a sample inlet is 250 ℃, 99.999 percent high-purity helium gas is used as carrier gas, the flow rate of the carrier gas is 1 mL/min, and the sample inlet adopts non-flow-division sample injection and operates in a constant flow mode.
(2) Mass spectrum conditions: the ion source voltage EI is 70 eV, the temperature of a column interface is 280 ℃, and the scanning ion range is 50-550 m/Z.
(3) Temperature rising procedure: the initial temperature is 50 deg.C, and the temperature is maintained for 2 min, and then raised to 260 deg.C at 20 deg.C/min and maintained for 2 min. Samples were taken 1. mu.L each time for manual sampling.
S3, analysis results show that the n-hexane soaking extracting solution contains 4 organic compounds, the total ion flow diagram of the compounds is shown in figure 2, and the compound list is shown in Table 1. The most abundant of them is caproic acid, accounting for up to 63.73%, and secondly caproic acid hexyl ester, accounting for 27.49%. All four compounds were identified by alignment using the NIST17 library and purchasing standard compounds.
Example 2: antrodia potential (GC-EAD) assay screening for compounds having physiological activity against Solenopsis invicta
The tentacle electrophysiological response of the ergates of the red fire ants to the compounds of the retro-thoracic stink glands of the apolygus legeliophis was tested by GC-EAD to screen potential repellent active substances of the red fire ants. The gas chromatography model is Agilent 7890B, and the chromatography conditions are as follows: HP-5ms (30 m, 0.25 mm inner diameter, 0.25 μm film thickness) capillary chromatographic column, high purity nitrogen as carrier gas, 250 deg.C of injection port, 320 deg.C of FID detector, 50 deg.C of column box temperature raising program, retention time of 2 min, and raising to 260 deg.C at 20 deg.C/min and maintaining for 2 min. The electrophysiological reaction of the retrosternal stinkbug gland to the feeler of the erges solenopsis was measured using an EAG insect feeler potential recorder (Syntech, netherlands). And (3) cutting off the antenna along the base part of the Gongant antenna, cutting a small opening at the tip end of the antenna, and then respectively connecting the two ends of the antenna to the positive and negative electrodes of the two glass capillary tubes of the EAG. The GC adopts a manual sample injection and non-flow distribution mode, the sample injection amount is 2 mu L, the sample flow distribution ratio of the GC to the EAG is 1:1, and more than 6 tentacles are repeated in each experiment.
The results showed that 2 substances at the GC-MS peak times of 5.526 min and 8.602 min were able to repeatedly elicit a strong electrophysiological response from solenopsis invicta (fig. 3), suggesting that these substances are potential substances that may have physiological activity against solenopsis invicta. Through NIST database peak shape comparison of GC-MS and verification of purchased standard products, the two substances with the antennal activity are respectively determined to be caproic acid and caproic acid hexyl ester.
Example 3: repellency test of stinky gland defensives against red imported fire ants
And (3) recording the repellent behavior characteristics of the retro-pectoralis compounds of the adelphocoris suturalis to the ergris solenopsis workers by adopting Ethovision XT11.5 software. Placing regular triangular filter paper sheets with the side length of 1 cm at two opposite corners of a culture dish with the diameter of 9 cm, dividing the culture dish into a control area and a treatment area by using a vertical line formed by intersecting two pieces of filter paper, dripping 2 microliters of a pure compound on one filter paper sheet, and taking the other filter paper as a blank control (a) in figure 4); 4 repeats per compound; then placing the worker ants of the solenopsis invicta into the culture dish in the middle of the culture dish, shooting and recording the moving track of the solenopsis invicta in the culture dish for 5 min by utilizing video software carried by Ethovion XT, automatically counting the moving time of the solenopsis invicta in two different areas in the culture dish, and utilizing SPSSt(ii) testing the differential significance of the activity time of the red imported fire ants in different areas: (P = 0.05）。
The results are shown in fig. 4, fig. 5 and table 2. The results show that hexanoic acid and hexanoic acid hexyl ester have stronger repelling effect on red imported fire ants, and the retention time of the red imported fire ants in the control area is obviously longer than that in the area treated by the compound (hexanoic acid: t = -10.579, df = 6, p)<0.001; hexyl caproate: t = -3.56, df = 6,p= 0.012), tendency that hexyl acetate and hexyl butyrate have no repellent effect on solenopsis invicta (ethyl acetateHexyl acid:p= 0.095; hexyl butyrate:p= 0.761) (fig. 4 (b)).
Claims (4)
1. Application of caproic acid and caproic acid hexyl ester in repelling Formica rufa is provided.
2. The use according to claim 1, wherein said solenopsis invicta is a solenopsis invicta worker ant.
3. A method for repelling solenopsis invicta is characterized in that caproic acid and/or hexyl caproate is applied to the area to be repelled, and caproic acid and/or hexyl caproate is used to repel solenopsis invicta.
4. The method of claim 3, wherein said applying is by spraying or pouring.
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