CN112042647A - Attractant for adult anoplophora chinensis - Google Patents

Attractant for adult anoplophora chinensis Download PDF

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CN112042647A
CN112042647A CN202010966399.4A CN202010966399A CN112042647A CN 112042647 A CN112042647 A CN 112042647A CN 202010966399 A CN202010966399 A CN 202010966399A CN 112042647 A CN112042647 A CN 112042647A
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adult
attractant
heptyloxy
anoplophora chinensis
volume ratio
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CN112042647B (en
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舒金平
彭瀚
张威
张亚波
刘亚宁
方宁鑫
李志红
王浩杰
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Research Institute of Subtropical Forestry of Chinese Academy of Forestry
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Research Institute of Subtropical Forestry of Chinese Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds

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  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

An attractant for adult anoplophora chinensis, belonging to the technical field of agriculture. The trapping agent is formed by mixing a compound and liquid paraffin according to the volume ratio of 1:2-10, wherein the compound comprises 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexene-1-ol and ocimene, and the volume ratio is 0-20:0-20:0-20:40-60:0-5:0-5:20-40:50-70: 50-70. Compared with the existing adult anoplophora chinensis attractant, the attractant has more trapping amount and simple and quick use method, and the trapping amount of the adult anoplophora chinensis can reach 13-16 per week of average trapping amount/trap. The insect attracting bait has strong targeting property, is not limited by the environment when in use, can be used for a long time, can trap pests in the whole period of emergence of adult anoplophora chinensis, and can effectively reduce the population density. Can also be used as an effective means for dynamically monitoring the population of the anoplophora chinensis.

Description

Attractant for adult anoplophora chinensis
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to an attractant for adult anoplophora chinensis.
Background
In recent years, the artificial forest in China is rapidly developed, and the area is sharply increased, but because the tree species are single and poor in diversity, the diseases and insect pests are frequently and seriously generated, and the disease and insect pests of the forest in the world are one of the most serious countries at present. Insects of the family longidae (Cerambycidae) are a major group of pests of which the most harmful and most difficult to control. Wherein the star longicorn is one of the most common longicorn species in subtropical regions of China, once the species occur, the star longicorn can cause destructive damage to economic forests and natural forests, and in recent years, citrus (Mandarin orange) is used in southern ChinaCitrus reticulata) Ephedra sinica Stapf in the southeast coast of the Yangtze province (Casuarina equisetifolia) Protective forest belt and down stream thin shell hickory nut (Carya illinoensis) The growing area is outbreaked to cause damage, which causes hundreds of millions of yuan of loss. Star of Tian niu (a Chinese character of xing)Anoplophora chinensis) The larvae are drilled into the trunk to eat, the concealment is strong, the larvae are the most difficult pests to control, and the trees are usually poor in growth and weak in tree vigor after being invaded by the larvae, and the larvae directly die of the whole tree when the pests are serious. At present, a convenient and efficient control method for anoplophora chinensis is not available, and researches on the control technology of the anoplophora chinensis are urgently needed.
At present, the main control methods of the anoplophora chinensis comprise artificial killing, chemical agent control and biological control by using natural enemies. The artificial trapping and killing cost is high, and the method is only suitable for insect pest control in a small range. The chemical agent has serious pollution to the environment, is easy to cause harm to non-target organisms or cause drug resistance of pests, and is not easy to be applied for a long time. The biological control is influenced by factors such as artificial propagation technology of natural enemies, shelf life, external environment during application and the like, and has poor stability and long effect period. The anoplophora chinensis larva has the characteristic of concealing harm, and the adult beetle is the only worm state with naked activity and obvious behavior trend of the anoplophora chinensis, and is also the most possible key worm state for implementing large-area prevention and control. Therefore, the method utilizes specific behaviors of adult searching for a mate, supplementing nutrition and the like, uses sex pheromone, lures plants or plant-derived attractants to lure and kill the adult longicorn, and is a good method for defending the enemy outside the forest and killing the adult before spawning harm. The trapping and killing method has the advantages of controlling the disease, protecting the surface, reducing the control range, saving labor resources, being easy to popularize and the like, and becomes the focus and key point of the research of the longicorn control technology.
The invention patent of Xuhua tide et al, "star longicorn attractant" (patent number: CN 201410030112.1) provides a star longicorn attractant which only traps 2 star longicorn in 2-month and-more-month forest tests; the invention patent of Yanjiangting and the like, namely 'a anoplophora chinensis imago attractant' (patent number: CN201510718829. X), is a anoplophora chinensis attractant reported at present, the highest insect attracting amount of which is about 10 heads/trapper/week, but the insect attracting amount is still difficult to play an effective role when being applied to monitoring and controlling production anoplophora chinensis, so that a more efficient anoplophora chinensis attractant is urgently needed to be developed.
Disclosure of Invention
In view of the problems in the prior art, the invention aims to design and provide an attractant for adult anoplophora chinensis. The adult anoplophora chinensis trap can achieve the average trap amount/trap/week of 16, the maximum trap amount can reach 23 heads/trap/week, is not limited by environmental factors, and can be used for a long time.
In order to achieve the purpose, the invention adopts the following technical scheme:
the attractant for the adult anoplophora chinensis is characterized in that effective components of the attractant comprise 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexene-1-ol and ocimene, wherein the volume ratio of the 4- (n-heptyloxy) butan-1-ol, the 4- (n-heptyloxy) butyraldehyde, the n-nonacosane, the beta-pinene, the myrcene, the phellandrene, camphene, the cis-3-hexene-1-ol and the ocimene is 0-20:0-20:0-20:40-60:0-5:0-5:20-40:50-70: 50-70.
The attractant for the adult anoplophora chinensis is characterized in that the volume ratio of 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexene-1-ol and ocimene is 5-15:5-15:5-15:45-55:1-4:2-3:25-35:55-65: 55-65.
The attractant for the adult anoplophora chinensis is characterized in that the volume ratio of 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexene-1-ol and ocimene is 10:10:10:50:1:2:30:60: 60.
The attractant for the adult anoplophora chinensis is characterized by further comprising liquid paraffin, wherein the volume ratio of the effective components to the liquid paraffin is 1: 2-10.
The invention has the following beneficial effects:
1. compared with the existing adult anoplophora chinensis attractant, the attractant has more trapping amount and simpler and quicker use method, and the average trapping amount of the adult anoplophora chinensis can reach 16 insects/trap/week. The method aims at trapping, killing and preventing the only adult anoplophora chinensis exposed outside the trunk of the plant, is an active exploration and practice for the leading-edge science and technology, and has important scientific value and social value.
2. The attractant has strong target property and obvious trapping and killing effect on adult anoplophora chinensis. The attractant is not limited by environment and can be used for a long time, and pests can be trapped in the whole period of emergence of adult anoplophora chinensis.
3. The attractant not only can effectively reduce the population density, but also has the function of monitoring the insect situation, and has strong specificity and high precision. Clean and sanitary, and environment-friendly.
Drawings
FIG. 1 is a graph showing the relative reaction values of EAG of adult anoplophora chinensis to attractant;
FIG. 2 is a graph showing the results of the intercropping effect of adult anoplophora chinensis attractant.
Detailed Description
The invention will be further illustrated by the following specific examples and the accompanying drawings.
Example 1:
9 compound monomers (4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene) are respectively diluted to 0.001, 0.01 and 0.1 muL/muL by liquid paraffin. Adult athetis (10-15 days of emergence) were starved for 5 h prior to testing, the tentacles were cut along the base of the adult tentacles and the flagellar tips were cut approximately 0.5 mm, and the tentacles were mounted on the EAG system electrodes using forceps and conductive gel (Spectra, Parker Laboratories). The AC/DC UN-6 amplifier was connected in DC mode to a PC terminal (Syntech Laboratories) equipped with the EAG 2.0 program using AgCl silver plated wire connection. Humidified air (500 mL/min) filtered by activated carbon was continuously conducted to the antenna through a stainless steel duct (internal diameter 1 cm) with the odor tube about 1 cm from the antenna. And (3) extracting 2 mul of the compound by using a micro sampler, uniformly dripping the compound on a filter paper strip of 2 cm multiplied by 0.5 cm, putting the filter paper strip into a sample tube of 10 cm in length, and connecting a gas stimulation control device to the tail end of the sample tube. After the baseline of the potential response had stabilized, the antennae were stimulated at 30 s intervals for 0.5 s each, and each compound was tested on 5 antennae of insects of different sexes, with an average of 5 stimulations per sample. Using liquid paraffin as a control, the EAG relative response value was defined as the average of the measurements taken for each sample divided by the average of the control measurements taken at the first and end of the test.
TABLE 1 EAG relative response values of female and male adult Apriona stellatus to 9 compounds
Figure DEST_PATH_IMAGE001
Note: data in table are mean ± sem (n = 5); the different lower case letters in the same row represent significant differences (P <0.05), different capital letters in the same column indicate significant differences (P <0.05) (Duncan's multiple comparison method).
Test results show that nine substances, namely 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene can cause obvious antennal potential reaction of female or male adults at high concentration (Table 1).
Example 2:
the attractant is prepared by screening 9 compounds with attraction activity to anoplophora chinensis, namely 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexene-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60: 60. The attractant formula stock solution is respectively diluted into 2, 4, 6, 8 and 10 times of diluent (namely the attractant obtained in the embodiment 3-8) by utilizing liquid paraffin, and the beneficial effect of the invention is further verified by measuring the antenna potential reaction (EAG) of the adult anoplophora chinensis to the attractant formula.
Before the test, anoplophora chinensis (10-15 days of eclosion) adults were starved for 5 h, the tentacles were cut along the bases of the tentacles of the adults, and the flagellar tips were cut off by about 0.5 mm, and the tentacles were mounted on the electrodes of the EAG system using tweezers and conductive gel (Struble and Arn, 1984). The AC/DC UN-6 amplifier was connected in DC mode to a PC terminal (Syntech Laboratories) equipped with the EAG 2.0 program using AgCl silver plated wire connection. Humidified air (500 mL/min) filtered by activated carbon was continuously conducted to the antenna through a stainless steel duct (internal diameter 1 cm) with the odor tube about 1 cm from the antenna. And (3) extracting 2 mul of the compound by using a micro sampler, uniformly dripping the compound on a filter paper strip of 2 cm multiplied by 0.5 cm, putting the filter paper strip into a sample tube of 10 cm in length, and connecting a gas stimulation control device to the tail end of the sample tube. After the baseline of the potential response had stabilized, the antennae were stimulated at 30 s intervals for 0.5 s per stimulation time, and each compound treated was tested on 5 antennae of insects of different sexes, with an average of 5 stimulations per sample. Using liquid paraffin as a control, the EAG relative response value was defined as the average of the measurements taken for each sample divided by the average of the control measurements taken at the first and end of the test.
The results of the relative reaction values of adult anoplophora chinensis to the EAG of the lure with different concentrations are shown in figure 1, and the attractant formulas with different concentrations can induce the strong EAG reaction of the anoplophora chinensis (1: stock solution, 2:2 times of diluent, 3: 4 times of diluent, 4: 6 times of diluent, 5: 8 times of diluent and 6: 10 times of diluent in the figure). The result shows that the attractant formula has strong stimulation effect on anoplophora chinensis.
Example 3:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60: 60. The above compound and liquid paraffin were mixed in a volume ratio of 1: 1.
Example 4:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60:60, and the compound is mixed with liquid paraffin in a volume ratio of 1: 2.
Example 5:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60:60, and the compound is mixed with liquid paraffin in a volume ratio of 1: 4.
Example 6:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60:60, and the compound is mixed with liquid paraffin in a volume ratio of 1: 6.
Example 7:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60:60, and the compound is mixed with liquid paraffin in a volume ratio of 1: 8.
Example 8:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 10:10:10:50:1:2:30:60:60, and the compound is mixed with liquid paraffin in a volume ratio of 1: 10.
Example 9:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 20:20:20:60:5:5:40:70:70, and the compound is mixed with liquid paraffin in a volume ratio of 1: 3.
Example 10:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 5:5:5:45:1:2:25:55:55, and the compound is mixed with liquid paraffin in a volume ratio of 1: 3.
Example 11:
4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene in a volume ratio of 15:15:15:55:4:3:35:65:65, and the compound is mixed with liquid paraffin in a volume ratio of 1: 3.
Example 12:
the volume ratio of the 4- (n-heptyloxy) butan-1-ol to the 4- (n-heptyloxy) butanal is 1: 1.
Example 13:
the volume ratio of 4- (n-heptyloxy) butan-1-ol to 4- (n-heptyloxy) butan-aldehyde is 1:1, and the above compound is mixed with liquid paraffin in the volume ratio of 1: 10.
Example 14:
the volume ratio of myrcene to phellandrene to beta-pinene to ethanol is 1:1:1: 1.
The application method comprises the following steps: a10 ml centrifuge tube is used as a core inducing carrier (Synergy semiconductors Corp.) and 9 small holes with the diameter of 1 cm are punched on the wall of the centrifuge tube to ensure that the core inducing compound can be fully volatilized and diffused, and a blank centrifuge tube is used as a control. A crossed baffle type longicorn trap (BF-I type longicorn trap, Hangzhou Ferumon Biotechnology limited) is used for trapping adult longicorn, and water and detergent (Guangzhou Libai corporation) are added into a trap collecting bottle to prevent the trapped longicorn from escaping. The spacing between traps was 30 m, the lower collector bottle was 1 m from the ground, 3 replicates were set for each treatment. 1 ml of core attracting formula is added into each core attracting carrier, investigation is carried out once every 3 days, meanwhile, a new core attracting agent is replaced, and information such as the number of anoplophora chinensis, the male-female ratio and the like caught by each trap is recorded. Results are shown in FIG. 2 (formulations 1-12 are attractant combinations of examples 3-14). The woodland trapping test of the inventive adult Nepeta arvensis attractant was carried out on the coastal shelter forest of the town of Cao, Yao, Yu Yao, Zhejiang province.
The mean trap amounts of the formulations of examples 3-11 of the invention were statistically significantly different from the blank control (P<0.01 ({ dot over (b) }) lower case letters indicate significant difference (B) }P<0.05), One-WayANOVA One-way analysis of variance and multiple comparisons (LSD) were performed using statistical software. The formula of the invention in the embodiment 3-11 has obvious attraction effect on the adult anoplophora chinensis, and can be used as the attractant for the adult anoplophora chinensis.
The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, and any simple modifications or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention are within the scope of the present invention.

Claims (4)

1. The attractant for adult anoplophora chinensis is characterized in that effective components of the attractant comprise 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butyraldehyde, n-nonacosane, beta-pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene, wherein the volume ratio of the 4- (n-heptyloxy) butan-1-ol, the 4- (n-heptyloxy) butyraldehyde, the n-nonacosane, the beta-pinene, the myrcene, the phellandrene, camphene, the cis-3-hexen-1-ol and the ocimene is 0-20:0-20:0-20:40-60:0-5:0-5:20-40:50-70: 50-70.
2. The attractant for adult anoplophora chinensis benth as claimed in claim 1, wherein the volume ratio of 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, β -pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene is 5-15:5-15:5-15:45-55:1-5:2-5:25-35:55-65: 55-65.
3. The attractant for adult anoplophora chinensis as claimed in claim 1, wherein the volume ratio of 4- (n-heptyloxy) butan-1-ol, 4- (n-heptyloxy) butanal, n-nonacosane, β -pinene, myrcene, phellandrene, camphene, cis-3-hexen-1-ol and ocimene is 10:10:10:50:1:2:30:60: 60.
4. The attractant for adult anoplophora chinensis as claimed in claim 1, further comprising liquid paraffin, wherein the volume ratio of the effective component to the liquid paraffin is 1: 2-10.
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