CN111543432B - Cow dung-source attractant for preventing and treating Holotrichia parallela imagoes - Google Patents
Cow dung-source attractant for preventing and treating Holotrichia parallela imagoes Download PDFInfo
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- CN111543432B CN111543432B CN202010014036.0A CN202010014036A CN111543432B CN 111543432 B CN111543432 B CN 111543432B CN 202010014036 A CN202010014036 A CN 202010014036A CN 111543432 B CN111543432 B CN 111543432B
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- holotrichia parallela
- cow dung
- butyric acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a cow dung attractant for controlling Holotrichia parallela imagoes in the technical field of biological control of pests. The attractant is Butyric acid (Butyric acid), the solvent is paraffin oil, and the mass fraction of the solute is 10%. The cow dung-source attractant for preventing and treating the Holotrichia parallela imago has good prevention and treatment effects on Holotrichia parallela, and can not cause the Holotrichia parallela to generate resistance; the preparation method is simple, the preparation cost is low, the method is harmless to human and livestock, the environment is friendly, and the method has important significance for comprehensively treating the Holotrichia parallela.
Description
Technical Field
The invention belongs to the technical field of biological control of pests, and particularly relates to a cow dung-derived attractant for controlling Holotrichia parallela imagoes.
Background
The Holotrichia parallela occurs for 1 generation in 1 year, larvae are 3 years old, wherein the 3 years old has the longest calendar period and the largest food consumption, the pest period almost runs through the whole growing season of crops, and in addition, the Holotrichia parallela is latent in soil to cause pest, so that the control difficulty is greatly increased, and the Holotrichia parallela is a soil pest which is acknowledged in the world and is difficult to control. Because the larvae of the Holotrichia parallela are hidden underground as harmful pests, comprehensive prevention and control are carried out by adopting the principle of combining underground and overground prevention and control and combining larvae and adult prevention and control, and the adult prevention and control can achieve the effect of twice with half the effort.
The Holotrichia parallela imago has oviposition preference to host plants, has stronger tendency to insufficiently decomposed organic fertilizers and putrefactive organic substances, and likes to lay eggs in the Holotrichia parallela imago; the application of the organic fertilizer which is not fully decomposed often causes the grubs to be seriously generated, and the attractability of the fully decomposed organic fertilizer to the scarabs is greatly reduced. Another research shows that the induction effect of the cow dung leaching liquor on the scarab beetles is obviously stronger than that of the castor leaf leaching liquor and the chemical attractant, which shows that the induction effect of the putrefactive organic matters on the adult scarab beetles is probably stronger than that of host plant volatile matters. Therefore, the research on the induction of the Holotrichia parallela imagoes by the cow dung source volatile matters has important significance for the ecological prevention and treatment of Holotrichia parallela.
Disclosure of Invention
The invention aims to provide a cow dung source attractant for preventing and controlling Holotrichia parallela imagoes so as to improve the effect of comprehensively treating Holotrichia parallela.
The main components in the cow dung volatile matter are obtained through a GC-MS test, the electrophysiological activity of the Holotrichia parallela on the components of the cow dung volatile matter is measured through an insect antenna potential test, and the volatile matter with strong electrophysiological activity is subjected to an attraction effect test in a field.
The effective component of the attractant is Butyric acid (butyl acid).
Preferably, the mass fraction of butyric acid is 10%.
The preparation method of the cow dung-source attractant for preventing and treating the Holotrichia parallela imago comprises the step of diluting Butyric acid (butyl acid) into a Butyric acid solution with the mass fraction of 10% by taking paraffin oil as a solvent.
The cow dung-source attractant for preventing and treating the Holotrichia parallela imago is used for attracting the Holotrichia parallela imago.
Compared with the prior art, the invention has the following excellent effects: the cow dung-source attractant for preventing and treating the Holotrichia parallela imago has good prevention and treatment effects on Holotrichia parallela, and can not cause the Holotrichia parallela to generate resistance; the preparation method is simple, the preparation cost is low, the method is harmless to human and livestock, the environment is friendly, and the method has important significance for comprehensively treating the Holotrichia parallela.
Drawings
FIG. 1 shows the antennal potential response of Holotrichia parallela male and female testworms to 28 volatiles.
In FIG. 2, (A) indicates the response of Holotrichia parallela adults to the antennal potential of Butyric acid (Butyric acid) solutions of different concentrations; (B) refers to the meteorological chromatographic peak of key volatile matters of the non-decomposed cow dung.
FIG. 3 refers to field trapping results for Butyric acid (Butyric acid) and control.
Detailed Description
The present invention will be described in detail below with reference to the drawings and examples, but the practice of the invention is not limited thereto.
Example 1 GC-MS analysis of cow dung extract fractions
And analyzing both the non-decomposed and decomposed cow dung. Gas chromatograph: shimadzu GCMS-QP2010SE type; a chromatographic column: RTx-5MS (30m × 0.25mm i.d., SGE) type capillary column. Temperature rising procedure: the initial temperature is 50 ℃, and the temperature is kept for 3 min; raising the temperature to 190 ℃ at a speed of 20 ℃/min, and keeping the temperature for 3 min; the temperature was raised to 280 ℃ at 5 ℃/min and maintained for 5 min.
The results of GC-MS of the non-decomposed and decomposed cow dung are shown in tables 1 and 2, respectively.
TABLE 1
TABLE 2
It can be seen that a total of 12 components were obtained in the non-decomposed cow dung and 11 components were obtained in the decomposed cow dung.
Example 2 Acertonemal potential reaction of Holotrichia parallela on different compound monomers
A total of 28 types of the cow dung volatile matters in example 1 and the plant volatile matter monomers which were proved to have an attracting effect on the tortoise shells in the research results of others were subjected to the insect antenna potential reaction test. The 28 volatile monomers were diluted to 0.1% strength test solution using paraffin oil as solvent. Selecting Holotrichia parallela imago active in plastic basin, placing on filter paper, rapidly cutting off tentacle base under dissecting mirror with scalpel, and mixing the tentacle base with conductive liquid (containing 750mg/ml NaCl, 35mg/ml KCl, 29mg/ml CaCl 2.2Hh)2O in water) with the tip of the antenna nested in the recording electrode. The electrode is internally provided with silver-silver chloride wires and connected with a signal amplifierThe signal was connected through a probe monitoring amplifier and the data recorded and analyzed using the EAG2000 software. HPLC grade paraffin oil was the blank control. mu.L of the sample was applied to the cut filter paper strip using a pipette and after the solvent had evaporated off the filter paper strip (approximately 20s), the filter paper strip was placed in a Pasteur tube. Electrophysiological responses of the Holotrichia parallela tentacles to each sample were recorded using EAG2000 software. Each stimulation time was 0.2s, the stimulation interval was 30s, and the flow rate of humidified air was 500 mL/min. The samples were tested in a random fashion, 8 antennae per sample, and the assay was repeated 3 times on each antenna, 10 samples per antenna.
The results are shown in FIG. 1.
As can be seen, the comparison of the difference between the response value of each compound and the response value of the control by Dunnett analysis method respectively shows that the response value of the male test insect to Butyric acid (butyl acid) is very significantly higher than that of the control (p < 0.001); while other volatiles such as Methanol (Methanol), Estragole (Estragole), carene (carene), linalool (linalool), indole (indole), p-cymene (p-cymene), camphene (camphene), nonanal (nonanal) showed only significant differences in response values compared to the control (p < 0.05). For female test insects, Butyric acid (Butyric acid), indole (indole), and camphene (camphene) alone were significantly higher than the control. Therefore, Butyric acid (Butyric acid) has very obvious attraction activity to male and female testworms of Holotrichia parallela.
Example 3 Tacromet response of Holotrichia parallela to butyric acid solutions of different concentrations
Butyric acid (Butyric acid) was diluted to solutions of 10%, 1%, 0.1%, 0.01%, and 0.001% by mass respectively using paraffin oil as a solvent, and the respective gradient solutions were subjected to the insect antenna potential test as in example 2.
The results are shown in FIG. 2.
It can be seen that the Holotrichia parallela has electrophysiological activity on Butyric acid (Butyric acid) solutions of various concentrations, and the higher the concentration is, the stronger the electrophysiological activity is.
Example 4 field trapping effect of cow dung-derived attractant on Holotrichia parallela imagoes
According to the former occurrence rule of Holotrichia parallela in the New county City of Henan province in China, the research is carried out at the early peak of Holotrichia parallela. The two treatments are respectively Butyric acid (butyl acid) solution with the mass fraction of 10% and CK (hollow lure). Each treatment was repeated 20 times, i.e. 20 green half-full basin traps, for a total of 40 traps. The traps were arranged randomly with each trap height of 1.5m and a distance of 10m between traps, and a suitable amount of detergent was added to the water in the basin. 1mL of Butyric acid (Butyric acid) solution is added into each green natural rubber stopper or the state of an empty lure core is kept, and then the rubber stopper is horizontally fixed on a small device of which the basin trapper floats on the water surface. The trapping period is 7/2019 to 8/6/2019. The experimental site is a field in which both peanuts and corns are in the seedling stage in the Xinxiang city of Henan province. According to the habit of emergence of the Holotrichia parallela every two days, the number of the trapped insects is counted by taking the trap as a unit.
The test results are shown in fig. 3.
It can be seen that Butyric acid (Butyric acid) trapped significantly more of the Holotrichia parallela adults than the control (p < 0.05). In the 1 st to 7 th surveys, adult Holotrichia parallela is active in the peak period, the Butyric acid (butyl acid) induces a larger amount of insects, and 293 adult Holotrichia parallela is induced at the highest time. Then the field insect quantity is reduced, the activity is reduced, and the test insects caught by the butyric acid are reduced. The blue circle in the graph indicates the result when the statistic was rainfall, indicating that rainfall seriously affects the attracting effect of Butyric acid (butyl acid). Adults began to emerge at 20:10 and began to feed and mate after the host search was completed quickly. The mating time is 1h to 1h 30 min. In the absence of rainfall, Holotrichia parallela mated frequently. Then, the leaves were fed until 22: 00. A series of results show that the Butyric acid (Butyric acid) solution has a strong attraction effect on Holotrichia parallela adults, and can be used as a novel attractant for preventing and controlling Holotrichia parallela.
Claims (1)
1. The application of the cow dung source attractant butyric acid for preventing and treating the Holotrichia parallela imago is characterized in that the butyric acid is used for attracting the Holotrichia parallela imago.
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Citations (4)
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|---|---|---|---|---|
| SU952190A1 (en) * | 1978-06-16 | 1982-08-23 | Полтавский Научно-Исследовательский Институт Свиноводства | Composition for protecting forage grain against granary pests |
| CN1056617A (en) * | 1990-05-22 | 1991-12-04 | 大连市环境卫生科学技术研究所 | Deodorization combined material for killing flies and insect |
| CN101268786A (en) * | 2008-04-18 | 2008-09-24 | 浙江大学 | Winged insect attractant |
| CN103876105A (en) * | 2014-03-24 | 2014-06-25 | 天津春发生物科技集团有限公司 | Soy sauce essence and preparation method thereof |
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- 2020-01-07 CN CN202010014036.0A patent/CN111543432B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU952190A1 (en) * | 1978-06-16 | 1982-08-23 | Полтавский Научно-Исследовательский Институт Свиноводства | Composition for protecting forage grain against granary pests |
| CN1056617A (en) * | 1990-05-22 | 1991-12-04 | 大连市环境卫生科学技术研究所 | Deodorization combined material for killing flies and insect |
| CN101268786A (en) * | 2008-04-18 | 2008-09-24 | 浙江大学 | Winged insect attractant |
| CN103876105A (en) * | 2014-03-24 | 2014-06-25 | 天津春发生物科技集团有限公司 | Soy sauce essence and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| Attraction of dung beetles to herbivore dung and synthetic compounds in a comparative field study;Christin Wurmitzer等;《Chemoecology》;20171231;第27卷;第75–84页 * |
| 暗黑鳃金龟引诱剂的筛选及大田效果评价;李晓峰等;《中国会议》;20191023;第160页 * |
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