CN105530811A - Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius - Google Patents
Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius Download PDFInfo
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- XDLDASNSMGOEMX-UHFFFAOYSA-N benzene benzene Chemical compound C1=CC=CC=C1.C1=CC=CC=C1 XDLDASNSMGOEMX-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/10—Catching insects by using Traps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a method for controlling pine wood nematode by installing, at regular intervals, net type traps including an attractant composition for attracting Monochamus alternatus and Monochamus saltuarius, which are pollinating insects of pine wood nematode, and capturing, within the traps, the attracted Monochamus alternatus and Monochamus saltuarius. The present invention can constantly and effectively capture Monochamus alternatus and Monochamus saltuarius over a whole generation by forming a net-type trap system at a control area on the basis of the physiological, ecological, and behavioral characteristics of Monochamus alternatus and Monochamus saltuarius which do not show active scattering mobility, and thus can control the damage caused by pine wood nematode through the medium of Monochamus alternatus and Monochamus saltuarius and can forecast whether or not damage will occur.
Description
Technical field
The present invention relates to the control of pine nematode, particularly by monitoring and catch the preventing and controlling pine wilt disease method that the vector insect Monochamus alternatus of pine nematode and the black longicorn of dragon spruce realize in a large number.
Background technology
Pine wood nematode (Pinewoodnematode, Bursaphelenchusxylophilus) causes pine nematode (PineWiltDisease to pine tree, Korean pine, korean pine etc.; Pine tree wilt disease) insect.Pine nematode is also called pine tree acquired immune deficiency syndrome (AIDS), due to the morbidity of pine wood nematode invasion pine tree, infected grove invades that within 6 days, to play leaf sagging, within 20th day, play leaf to wither, after 30 days, sharply become red, 100% is withered, is fatal disease (the state-run forest academy of sciences of http://www.kfri.go.kr/ Korea S).
Pine wood nematode is soil species (Knowlesetal., 1983 of North American continent; Dwinell, 1993), the autochthonal seeds major part of the U.S., Canada, Mexico etc. has resistance (Kimetal. to pine nematode, 2003), the seeds of contrary other countries do not have this species resistance, receive serious disease, and current this injury is also in extensively carrying out.
According to related data, find at the beginning of Japan 1900 disaster-stricken after, disaster area is constantly spread, current pine tree and Korean pine almost all completely annihilated (Mamiya, 1988; Kishi, 1995), China is after nineteen eighty-two Late Cambrian, and pine tree, Korean pine, masson pine are subject to serious disease, and Taiwan is after Late Cambrian in 1985, and Ryukyu pine, Korean pine, Taiwan two Ye Song etc. are in dangerous situation (Enda, 1997) of becoming extinct.
Korea S, since Jin Jing mountain, Pusan in 1988 finds disease, disaster area constantly expands, and disease occurs prefecture district of maximum Shi You67Ge city.Implemented preventing and controlling pine wilt disease special law and preventing energetically afterwards, disaster area reduces to 40 Duo Geshijun districts, and disaster area is reduced to 3,547ha in 2010 from 7,871ha in 2006, present downward trend.But disaster area bounce-back in 2011, within 2012, note down 5, (Forest Diseases insect in 2012 occurs to look into investigation annual report in advance 286ha, the state-run forest academy of sciences of Korea S), the occurring area of 2013 increased by 119% than upper one year, reached 11,550ha,, there is area and also expand road, whole 13 cities of Korea S to, 59 prefecture districts of city in the greatest measure since record investigation.Southern areas often disaster-stricken such as the regional Jizhou of original generation, celebrating south, capital city and its environs Yang Ping, Henan, Lian Chuan, add flat, loyal Bei Zhongzhou, wide area city, Guangzhou has newly gone out damage (Forest Diseases insect in 2013 occurs to look into investigation annual report, the state-run forest academy of sciences of Korea S in advance).As mentioned above, the trend that the disaster area of pine wood nematode and Infected regions are increasing gradually, its situation occurred is complicated, and the time withered after infecting is very short, and the seriousness of problem continues to increase.Find pine wood nematode in korean pine 2 in the national reservation of mountain, North Korea, Soul on the 12nd June in 2014, cause the high-pressure of relevant departments.
The non-constant of self locomotivity of nematoda, the locomotivity of pine wood nematode outside tree is also very poor, is moved on other trees by vector insect.The vector insect of main utilization has a day Bos insect, there is Monochamuscarolinensis North America, M.mutator, M.scutellatus, M.titillator (Linit, 1988) etc., there is M.galloprovincialis (Sousaetal. in Portugal, 2001), there are M.sutor (Pajaresetal., 2013) in Spain and Sweden.It was reported, Korea S and vector insect that is Japanese, Chinese, Taiwan are Monochamus alternatus (Monochamusaternatus) (MamiyaandEnda, 1972; Kishi, 1995), it is reported, northeastern Japan area, the black longicorn of dragon spruce (Monochamussaltuarius) is medium pine wood nematode (TakizawaandShoji, 1982) also.Recently, the black longicorn of the dragon spruce of pine tree and korean pine also medium pine wood nematode (Kwonetal., 2006 have also been found out Korea S is domestic; KFRI, 2007), especially serious to the disease of korean pine.Pine wood nematode is attached to it specific longicorn, by eating (Maturationfeeding after longicorn is to the bark of healthy branch, adultfeeding, adult ingests) time and tear when bark is laid eggs occur wound, infect to (WingfieldandBlanchette, 1983 on the tree of health; Edwardsandlinit, 1992).
So far, Korea S is to the research aspect of pine nematode, and Main way not for the pine wood nematode almost not having mobility, perch on host tree, but is studied for the Monochamus alternatus of these nematodes of medium.Along with the fact of the black longicorn of dragon spruce also medium nematode is revealed, the insect of this kind is also being studied.Current existing research has sprout wings in the Regional Distribution (Kwonetal., 2006) of two kinds of insects and point period de-cocoon characteristic (Kimetal., 2003; Hanetal., 2009), individual other form (Leeetal., 2004; Hanetal., 2007), distribution (Chungetal. in pine tree, 2003), pine wood nematode departs from (Kimetal., 2009) from the mobile of vector insect, there is (Kimetal. in the pine wood nematode disease relevant to the mobile behavior of vector insect, 2011), the basic research of the growth relevant to coniferous tree kind and the biology such as growth and reproduction (Hwangetal., 2008), ecological aspect.
As the research of the prevention and controls aspect of pine wood nematode, (KFRI centered by the underlying biological characteristic sum exploitation anti-therapy of actual field two aspects of the pathogenesis and relevant essential factor of verifying pine nematode, 2009) biological control method, chemical prevention and control method, physical control method, is divided into study.First, biological control method has and infects wooden diagnosis, natural enemy and utilize that method, entomopathogenicity are mould utilizes method, resistant varieties cultivation etc.Chemical prevention and control method has based on the biology of vector insect, Ecological Basis research, for period of sprouting wings the anti-therapy of chemical insecticide (ground is broadcasted sowing, aviation broadcast sowing), fell the selection etc. of fumigating system (comprising the exploration that biological source material fumigates material), nematode and vector insect control material.Physical method has the felling for morbidity wood or dead and drying tree to destroy method, felling crush method, region isolation method (pine tree is moved in monitoring) (KFRI, 2009) etc.But although drop into a lot of manpower and time, expense at multiple fields, in the problem of the most essence of control pine wood nematode, be not also resolved method.
Insect is by light, sound, smell etc., and exchange of information between each individuality also passes on intention, wherein passes on the semiochemicals of intention to be called communication compound by smell.Communication compound is divided into pheromones (Pheromone: the material bringing out the special physiological reaction of insect of the same race or behavior) and allelochemical (Allelochemicals: the material to other kind effects) by communication theme.Wherein " pheromones (Pheromone) " is a small amount of in animal body manufacture, discharges into the atmosphere as smell, the opposite sex in of the same race or of the same race in individuality between the material that conveys a message.In the pheromones of secretion for the purpose of conveying a message between animal individual, it is the most extensive that insect pheromone is studied, by insect in the face of the reaction (by the direct action reaction of central nervous system and the change of reproduction and endocrine system) stimulated is divided into physiological change pheromones (Primerpheromone) and the organic pheromones of action (Releaserpheromone).In addition, allelochemical be divided into the allomone of the individuality being conducive to secretion signal material (Allomone), be conducive to the kairomone (Kairomone) of the individuality received, the synomone (Synomone) all favourable to the individuality of the individuality sent and reception.Insect pheromone is divided into the considerable kinds such as sex pheromone (sexpheromone), assembly pheromone (aggregationpheromone), alarm pheromone (alarmpheromone), pheromones of leading the way (trailpheromone), dispersed information element (epideicticpheromone), class polarization pheromones (castedifferentiationpheromone) (the academic total book 9 of agriculture life science institute, the 13rd chapter outside chemical communication, 2005) by between individuality in the face of stimulating the insect lipids shown to react.
Domestic in Korea S, utilizing these sense of smell inducing substances to prevent and treat, the experiment of pine nematode has with Monochamus alternatus is the experiment of object and the experiment that is object with the black longicorn of dragon spruce.Be that the experiment of object has " exploitation of pine wood nematode integrated control technique " of Forestry Academy in 2002 to use benzene (benzene) with Monochamus alternatus, australene (α-pinene), turpentine oil (terpentine), the experiment of pine tree essential oil etc., the mixture of the use australene that developmental research institute of Pusan in 2007 does and ethanol, the ipsenol (ipsenol) of assembly pheromone and the experiment of ipsdienol (ipsdienol) of Limnoria is belonged to as Ips, study 2004 to 2008, in the Korean-Chinese joint research of " the preventing and controlling pine wilt disease technique study " of state-run forest institute of the Korea S of 2009 annual reports, the Monochamus alternatus of China is used to lure the experiment etc. of material { principal component: australene (about 86.7%)+nopinene (about 11.5%) }.With the black longicorn of dragon spruce for the experiment (" Ipsenol of the black longicorn of dragon spruce, Ipsdienol attractant effect ", 2008) that plant medical science section uses ipsenol and ipsdienol confirmation attractant effect is learned by the experiment You Zhong Beijing University that object does.In addition, the experiment that China does has use 2-(11 oxygen)-1-ethanol (2-(undecyloxy)-1-ethanol), and the composition of australene and ethanol, confirms the experiment (Teale of the attracting ability to Monochamus alternatus, etal., 2011).
Although these experiments show and lure possibility to Monochamus alternatus and the black longicorn of dragon spruce, as prevention and controls, its effect is not also confirmed.
[prior art document]
[patent document]
(patent document 1) 1. Korean Patent discloses No. 10-2008-0061309
(patent document 2) 2. Korean Patent discloses No. 10-2010-0120430
(patent document 3) 3. Korean Patent discloses No. 10-2014-0025665
(patent document 4) be No. 10-0767783rd, Korean Patent Registration 4.
[non-patent literature]
(non-patent literature 1) 1.StephenA.Teale, JacobD.Wickham, FeipingZhang, JunSu, YiChen, WeiXiao, LawrenceM.Hanks, andJocelynG.Millar.AMale-ProducedAggregationPheromoneofM onochamusalternatus (Coleoptera:Cerambycidae), aMajorVectorofPineWoodNematode.J.Econ.Entomol.104 (5): 1592-1598 (2011).
Summary of the invention
The technical problem that invention will solve
The present invention proposes to solve the problem, and its object is to provides a kind of preventing and controlling pine wilt disease method, utilizes the trap system containing luring with composition, monitoring or catch Monochamus alternatus and the black longicorn of dragon spruce in a large number.The present invention is the ecophysiological traits of three based on Monochamus alternatus and the black longicorn of dragon spruce, and the behaviors and habits based on them is derived.The flight range of Monochamus alternatus, between 1 year centered by dead and drying tree or disaster site, be present in almost within 1km, disaster-stricken range of scatter also only has 2 ~ 3km, does not have active flight mobility.Based on the luring of the trap of the present invention (containing luring with composition) that characteristic in this behavior and the present invention confirm, capture ability, the invention provides a kind of can monitoring and catch the trap system of Monochamus alternatus and the black longicorn of dragon spruce in a large number and utilize this preventing and controlling pine wilt disease method.
For the technical scheme of dealing with problems
A kind of preventing and controlling pine wilt disease method that the invention provides monitoring based on Monochamus alternatus and the black longicorn of dragon spruce and catch in a large number, it has the trap netted setting at regular intervals of luring with composition, described luring contains 2-(11 oxygen)-1-ethanol, australene and ethanol with composition.
Described luring can comprise more than to be selected from (±)-3-carene, ipsenol, ipsdienol, n-pentacosane, n-nonacosane a certain with composition.
Described luring is housed in macromolecule material pouch with composition, is arranged on the outside or inside of described trap.In most preferred embodiment, described macromolecule material pouch comprises inside and is filled with cotton polyethylene pipe (strawpolyethylenefiltertube), described luring is used in composition, australene and ethanol are filled in described internal pouch, and 2-(11 oxygen)-1-ethanol is filled in described polyethylene pipe.
Described trap is the vertical continuous funnel trap (multifunneltrap) connecting 5 ~ 10 funnel traps and formed.Described trap be connected with bottom catch Monochamus alternatus and the black longicorn of dragon spruce catch bucket, there is lid topmost.
Described controlling object area be pine nematode do not occur area, clean area, disaster site adjoining area time, can by monitoring the generation of Monochamus alternatus and dragon spruce black longicorn time normal, control pine nematode.Here, by described generation monitoring, grasp the opportunity of suitably broadcasting sowing of ground control or aero control and be suitable for, can pine nematode be prevented and treated.
When described controlling object area is kainogenesis ground or the small-scale disaster area of pine nematode, centered by dead and drying tree or disaster site, trap is set, and in the region set with horizontal 500m ~ 2km, longitudinal 500m ~ 2km, to be captured as object, netted trap is set, in described extra-regional 100 ~ 200m place, around arranging 10 ~ 100 traps for the purpose of monitoring, by catching in a large number and extra-regional 2 disaster-stricken diffusion monitoring in region, control pine nematode.In addition, described controlling object area is the extensive disaster area of pine nematode, in the whole region in described disaster area, to be captured as object, nettedly arranges trap, by catching Monochamus alternatus and the black longicorn of dragon spruce in a large number, and control pine nematode.
Here, whether the spacing between described trap occurs by control area, the form of forest and disaster, suitably selects, preferably 20 ~ 100m scope.Wanting when there is not the regional monitoring disaster of disaster to be 70 ~ 100m, being diffused as 50 ~ 100m when object uses in disaster area to prevent and treat disaster, can to arrange within 50m during the high or Disaster degree height of forest density.
The effect of invention
The present invention is based on the characteristic in the physiology of the flight inactive Monochamus alternatus of mobility and the black longicorn of dragon spruce, ecology and behavior, in control area, netted trap system is set, sustainable, effectively lure, catch Monochamus alternatus on whole generation time and the black longicorn of dragon spruce.Thus the present invention can effectively monitoring by the pine wood nematode disaster of Monochamus alternatus and the black longicorn medium of dragon spruce, advance preventing disaster can be used in or prevent and treat the diffusion of disaster, and when there is disaster, by catching in a large number, can be used as prevention and controls and using.
Accompanying drawing explanation
Fig. 1 and Fig. 2 is trap system synoptic diagram of the present invention.
Fig. 3 uses the carrier of SPEFT to illustrate intention.
Fig. 4 is captured in the longicorn photograph of catching in bucket in the experiment in Yongchuan.
Fig. 5 is captured in the longicorn photograph of catching in bucket in the experiment of Pu item.
Fig. 6 is captured in the longicorn photograph of catching in bucket in the experiment in celebrating state.
Embodiment
At this, the present invention is described in detail.
1. lure and use composition
Of the present invention luring contains 2-(11 oxygen)-1-ethanol { 2-(Undecyloxy)-ethanol}, australene (α-pinene) and ethanol with composition.Described lure also to comprise with composition be selected from (±)-3-carene { more than a certain in (±)-3-carene}, ipsenol (ipsenol), ipsdienol (ipsdienol), n-pentacosane (n-pentacosane), n-nonacosane (n-nonacosane).
2-(11 oxygen)-1-ethanol is that longicorn (Monochamus) belongs to assembly pheromone, also known as making Monochamol.2-(11 oxygen)-1-ethanol is the assembly pheromone of the male release of longicorn, to the female of Monochamus alternatus and the black longicorn of dragon spruce with malely all have attractant effect, under the smell effect of this pheromones, find in the process of target and is caught by trap.
Australene, (±)-3-carene, ipsenol and ipsdienol allow the female and male material recognizing host's (pine tree class) of the individual and sexual maturity individuality of the sex immature of Monochamus alternatus and the black longicorn of dragon spruce, to Monochamus alternatus and the sex immature of the black longicorn of dragon spruce and the female of sexual maturity individuality with malely all have attractant effect.If the non-sexual maturity of sky bovine insect such as Monochamus alternatus and the black longicorns of dragon spruce, even if then sprout wings into adult, can not mating.After becoming adult, experiencing could mating between sexual maturing period.During this, by process of ingesting, injure (eating afterwards), become sexual maturity individuality.Here, the prematurity individual need of de-cocoon of sprouting wings looks for pine tree class newly careless, the materials such as its perception australene.
When plant is upset (infringement physically, withered process or withered state), ethanol can be discharged, and this material of individual perception is lured.Especially, very large to sexual maturity individuality active force from early days, affect australene, (±)-3-carene, the diffusion of ipsenol and ipsdienol, allow more individuality recognize.
N-pentacosane and n-nonacosane thing epidermis hydrocarbon, work when female and male mutual searching the other side, be placed in trap, contribute to catching.
Preferably, by 2-(ten one the oxygen)-1-ethanol of each trap containing 10 ~ 1000mg, the australene of 1 ~ 400g and the ethanol of 1 ~ 400g are advisable.If containing adding composition, be then advisable containing (±)-3-carene 1 ~ 400g, ipsenol 10 ~ 3,000mg, ipsdienol 10 ~ 3,000mg, n-pentacosane 0.1 ~ 10mg, n-nonacosane 0.1 ~ 10mg by each trap.
In the present invention, trap has lures with composition, " have " be here not limited to the inside of trap or adjacent have to lure with outside use composition.Of the present invention lure can be separated by with trap at regular intervals with composition arrange, relative to multiple trap one lured and be arranged on trap outside with composition, or relative to a trap, multiple luring is arranged on trap outside with composition." have and lure the trap with composition " of the present invention all comprises above-mentioned various forms.
2. carrier
Described luring preferably is filled in certain carrier with composition, possesses on trap.Described in filling, lure the carrier using composition, pouch (pouch) or the bag (bag) of macromolecule material can be used.In the present invention, below described " pouch " have nothing to do with form, as long as material is housed in inside, maintains the parts of sealing state, all comprise.Described macromolecule material can be low density polyethylene (LDPE) (lowdensitypolyethylene, LDPF), high density polyethylene (HDPE) (highdensitypolyethylene, HDPE) some, in thermoplastic polyurethane (thermoplasticpolyurethane, TPU).Here, the order of rate of release is HDPE>LDPE>TPU, environmentally with day/time, suitably select the carrier met, so that adjusting release quantity.
In addition, in order to regulate the rate of release of luring with composition, carrier comprises the polyethylene pipe (strawpolyethylenefiltertube, SPEFT) being filled with cotton and is advisable in described macromolecule material pouch.In most preferred embodiment, when using SPEFT, as most preferred embodiment, pheromones 2-(11 oxygen)-1-ethanol is filled in described SPEFT, and other compositions can be filled in the pouch as outer carrier.Use the carrier of this form, can discharge to lure for a long time and use composition.Carrier one example of SPEFT is used to represent in figure 3.As shown in Figure 3, the pouch (10) of carrier thing TPU material, PE pipe (20) has been put in its inside, is filled with compression cotton in the inside of PE pipe (20).
Put inside or the outside of luring and can be arranged on trap with the carrier of composition, if be arranged on outside, then not only can be arranged on the outside adjacent with trap, can also be arranged on and trap phase outside across a certain distance, can relative to multiple trap, arrange one at a certain distance and lure and use composition.
3. trap
Trap of the present invention can use the vertical continuous funnel trap (multifunneltrap connecting 5 ~ 10 funnel traps and formed; Lindgrentrap).The upper diameter of described funnel trap is 20cm ~ 30cm, and lower diameter is that 7cm ~ 14cm is advisable.Relative to the 12 continuous funnel traps (lindgrentrap) that conventional funnel trap upper diameter is 15cm, upper diameter is expanded about 2 times, make it to be easy to target acquisition insect, and reduce the number of trap, while maintaining trees shape, avoid entering of other insects as far as possible, only catch the Monochamus alternatus as target and the black longicorn of dragon spruce.
Best, the top connection cover of trap, the diameter of lid is that 30 ~ 40cm is advisable.Trap be connected with bottom catch Monochamus alternatus and the black longicorn of dragon spruce catch bucket.In most preferred embodiment, described in catch bucket length be 25 ~ 35cm, diameter is 10 ~ 15cm, especially length is 25 ~ 35cm, diameter is that 12 ± 3cm is better.Catch bucket inside described, there is anti-escaping and be advisable with funnel.Described anti-to escape by the wall of funnel and the angle of funnel be less than 45 degree, and the length of funnel is more than 6cm.From have leaking hole the 12 funnel traps formed with simple structure to catch bucket different, anti-escaping with funnel installed by bucket of catching of the present invention in inside, can maintain stable trapped state.
In addition, in the method to set up of trap, normally used 12 funnel traps need to arrange erecting bed on the ground, arrange in a hanging manner, cannot regulate height, and be subject to the restriction of landform when arranging, and the present invention utilizes hawser (rope) that trap is directly hung in the tree, can free adjustment height, aspect is set not by the influence of topography.
Be filled with the carrier of the present invention of luring with composition, preferably hang the outside being arranged on described trap.Here, carrier can be arranged on the optional position in described trap 5 ~ 10 funnel, can arrange the carrier of suitable number in a direction to four directions.Here, by each trap (comprising 5 ~ 10 funnels), 1 ~ 4 carrier is set and is advisable.
4. monitoring and prevention and controls
The flight range of Monochamus alternatus, between 1 year centered by dead and drying tree or disaster site, be present in almost within 1km, disaster-stricken range of scatter also only has 2 ~ 3km, does not have active flight mobility (the state-run forest academy of sciences of Korea S forest disease research class).Equally, after the black longicorn short distance flight of dragon spruce, find host plant (ecosystem characterization of pine wood nematode vector insect and natural enemy research on utilization, the state-run forest academy of sciences of Korea S) by walking, from this behavior, it should not have active flight mobility.The trees crown density (sunlight screening rate) of forest affect the mobility of longicorn too, and crown density and mobility have inverse relation.
The present invention is based on the characteristic in the physiology of this Monochamus alternatus and the black longicorn of dragon spruce, ecology and behaviouristics, define netted trap system by described luring with composition, carrier and trap.Fig. 1 and Fig. 2 represents the summary of trap system of the present invention.Trap of the present invention (100) at regular intervals (A) is arranged on controlling object area, especially, as shown in Figure 1 and Figure 2, by the form of four directions equidistant (A), arranges trap and is advisable.
Trap system of the present invention, containing luring the trap (100) using composition, (A) netted setting at a certain distance, by luring with composition, lures Monochamus alternatus and the black longicorn of dragon spruce to enter trap inside.Spacing (A) between trap is by control area, and whether woods form and disaster occur, and suitably select, and preferably 20 ~ 100m is advisable.Wanting when there is not the regional monitoring disaster of disaster, being suitable for 70 ~ 100m and being advisable, in disaster area, when being diffused as object use to stop disaster, being suitable for 50 ~ 100m and being advisable.What represent with redness in Fig. 2 is trap, and inducing substance spreads from trap, forms network effect in control area.Monochamus alternatus on whole generation time and the black longicorn of dragon spruce can be caught with composition by luring.
Trap system of the present invention, can use monitoring (Monitoring) respectively or simultaneously and catch in a large number (Mass-trapping), control pine nematode.
If monitoring, under following 3 kinds of situations, the generation of Monochamus alternatus and the black longicorn of dragon spruce time normal, can be monitored, control pine nematode:
(1) softwood forest that area does not occur that pine nematode had not occurred is arranged on, the generation of monitoring disaster.
(2) be arranged on and once pine nematode occurred and the softwood forest controlling the cleaning area of moving back completely, use as to the monitoring means of recurrence.
(3) be arranged on and kainogenesis ground or the adjacent regional softwood forest in disaster area, use as to the monitoring means of diffusion.
Described softwood forest especially comprises pine forests, korean pine woods, Korean pine woods.
By described generation monitoring, grasp ground control or aero control suitably broadcast sowing opportunity, for preventing and treating pine nematode.
When catching in a large number, under following 2 kinds of situations, can use from strategic level:
(1) in kainogenesis ground or the small-scale disaster area of pine nematode, use to stop the object of disaster-stricken diffusion.Here, centered by dead and drying tree or disaster site, trap is set, and in the region set with horizontal 500m ~ 2km, longitudinal 500m ~ 2km, to be captured as object, netted trap is set, in described extra-regional 100 ~ 200m place, around arranging 10 ~ 100 traps for the purpose of monitoring, by catching in a large number and extra-regional 2 disaster-stricken diffusion monitoring in region, control pine nematode.Preferably, trap is set centered by dead and drying tree or disaster site, and in the region set with horizontal 1km, longitudinal 1km, trap is set with 50 ~ 100m interval, the 150m place outside 1km, around arranging 16 ~ 20 traps.
Described " on a small scale disaster area " refers in a given area, and the disaster-stricken situation being confined to a part, compared with the disaster in control disaster area self, prevents 2 diffusions more important.
(2) in the extensive disaster area that pine nematode occurs, use as direct means of prevention.Here, trap is set comprehensively, centered by trap, allows pheromones smell spread, construct pheromones-network system, catch Monochamus alternatus and the black longicorn of dragon spruce in a large number, by direct control, deal with problems.The set-up mode of trap presses Tree Structure and the size of forest, geographic position, topographical conditions, and other environmental factors can flexible modulation be suitable for.Usually, the spacing between each trap is 20 ~ 100m, forest density or Disaster degree higher, the interval between described trap is less is advisable.Substantially, during the high or Disaster degree height of forest density, arrange described trap with interval within 50m, it arranges scope and can judge according to situation.Density and Disaster degree not serious time, arrange by (1) number method, but judge to arrange scope according to situation.
Here, by sharing with other means of prevention, the effect brought out the best in each other can be obtained.Other means of prevention comprise the multiple methods such as biological control method, chemical prevention and control method, physical control method, as long as known method all can comprise, especially can comprise the anti-therapy of chemical insecticide (ground is broadcasted sowing, aviation broadcast sowing).
Below, example by experiment, the present invention will be described in more detail.Experimental example is below an example of the present invention, and scope of the present invention is not limited to these experimental examples.
[experimental example 1]
In North Kyongsang Yongchuan, select to have and just to troop place in the pine tree of withered trees or dead and drying tree, trap was set on March 29th, 2014, investigates May 3.Employ 2 traps.
Employ 6 continuous funnel traps as trap, as carrier, consider the molecular characterization of sky Bos assembly pheromone, rate of release and stability, outside use TPU material, PE pipe has been put in inside.Assembly pheromone is filled in PE pipe, and australene and ethanol are directly put in TPU material.Employ with composition the composition be made up of 2-(11 oxygen)-1-ethanol 50mg, australene 20ml and ethanol 20ml as luring.
Its result is as shown in table 1 below, catches the longicorn photograph of barrel IT as shown in Figure 4.
[table 1]
[experimental example 2]
At North Kyongsang Pu item, select to have and just to troop place in the pine tree of withered trees or dead and drying tree, trap was set May 25, investigates to June 8.Altogether employ 10 traps.
The trap used is identical with experimental example 1 with carrier.As luring with composition, 5 traps employ the composition be made up of 2-(11 oxygen)-1-ethanol 100mg, australene 40ml and ethanol 40ml, all the other 5 traps employ the composition be made up of 2-(11 oxygen)-1-ethanol 200mg, australene 80ml and ethanol 80ml, has respectively put 2 times or 4 times to the composition of described experimental example 1.
Its result is as shown in table 2 below, catches the longicorn photograph of barrel IT as shown in Figure 5.
[table 2]
[experimental example 3]
In North Kyongsang celebrating state, select to have and just to troop place in the pine tree of withered trees or dead and drying tree, arranged trap June 26, lasting investigation so far.Altogether employ 6 traps.
The trap used is identical with experimental example 1 with carrier.As luring with composition, No. 1 and No. 2 traps employ the composition be made up of 2-(11 oxygen)-1-ethanol 10mg, australene 5ml and ethanol 5ml, No. 3 and No. 4 traps employ the composition be made up of 2-(11 oxygen)-1-ethanol 100mg, australene 25ml and ethanol 25ml, No. 5 and No. 6 traps employ the composition be made up of 2-(11 oxygen)-1-ethanol 1000mg, australene 250ml and ethanol 250ml.
Its result is as shown in table 3 below, catches the longicorn photograph of barrel IT as shown in Figure 6.
[table 3]
Described 3 regional experimental results, all actually in all experiments to lure, capture Monochamus alternatus in natural environment and the black longicorn of dragon spruce.Set each trap, because of landform, position, difference environmentally, there is the trap of successfully catching and fail the trap of catching, but the difference of the content of inducing substance does not cause the difference of catching.
Claims (amendment according to treaty the 19th article)
1. (amendment) a kind of monitoring based on Monochamus alternatus and the black longicorn of dragon spruce and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
The trap netted setting at regular intervals with macromolecule material pouch, in described macromolecule material pouch, be filled with 2-(11 oxygen)-1-ethanol, australene and ethanol;
Described pouch is triplen, and be filled with cotton polyethylene pipe comprising inside, described 2-(11 oxygen)-1-ethanol is filled in described polyethylene pipe, and described australene is filled in the pouch of thermoplastic polyurethane (TPU) material.
2. (amendment) monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described pouch is containing 2-(11 the oxygen)-1-ethanol of 10 ~ 1000mg, the australene of 1 ~ 400g and the ethanol of 1 ~ 400g.
3. (amendment) monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described pouch also comprises more than to be selected from (±)-3-carene, ipsenol, ipsdienol, n-pentacosane, n-nonacosane a certain.
4. (amendment) monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 3 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described pouch is containing (±)-3-carene 1 ~ 400g, ipsenol 10 ~ 3,000mg, ipsdienol 10 ~ 3,000mg, n-pentacosane 0.1 ~ 10mg, n-nonacosane 0.1 ~ 10mg.
5. (amendment) monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described pouch is arranged on the outside or inside of described trap.
6. (deletion)
7. (deletion)
8. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described trap is the vertical continuous funnel trap connecting 5 ~ 10 funnel traps and formed.
9. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 8 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described trap be connected with bottom catch Monochamus alternatus and the black longicorn of dragon spruce catch bucket, have lid topmost, the upper diameter of described funnel trap is 20cm ~ 30cm, and lower diameter is 7cm ~ 14cm.
10. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 9 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described bucket of catching comprises and inner anti-ly escapes with funnel, and described anti-to escape by the wall of funnel and the angle of funnel be less than 45 degree, and the length of funnel is more than 6cm.
The monitoring based on Monochamus alternatus and dragon spruce black longicorn of 11. (amendments) according to any one in claim 1 to 5 and claim 8 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is following some a) in c), by monitoring the generation of Monochamus alternatus and dragon spruce black longicorn time normal, and control pine nematode:
Softwood forest in the area that a) pine nematode do not occur
B) softwood forest in the cleaning area of moving back pine nematode has been controlled
C) kainogenesis ground adjoining area or the area adjacent with disaster area in softwood forest.
12. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Interval between described trap, not occurring in area or clean area is 70 ~ 100m.
13. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Interval between described trap is 50 ~ 100m in described adjoining area.
14. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
By described generation monitoring, grasp the opportunity of suitably broadcasting sowing of ground control or aero control and be suitable for, control pine nematode.
The monitoring based on Monochamus alternatus and dragon spruce black longicorn of 15. (amendments) according to any one in claim 1 to 5 and claim 8 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is kainogenesis ground or the small-scale disaster area of pine nematode, centered by dead and drying tree or disaster site, trap is set, and in the region set with horizontal 500m ~ 2km, longitudinal 500m ~ 2km, to be captured as object, netted trap is set, in described extra-regional 100 ~ 200m place, around arranging 10 ~ 100 traps for the purpose of monitoring, by catching in a large number and extra-regional 2 disaster-stricken diffusion monitoring in region, control pine nematode.
16. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 15 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Spacing between described trap is 50 ~ 100m, forest density or Disaster degree higher, the spacing of trap is less.
The monitoring based on Monochamus alternatus and dragon spruce black longicorn of 17. (amendments) according to any one in claim 1 to 5 and claim 8 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is the extensive disaster area of pine nematode, in the whole region in described disaster area, to be captured as object, nettedly arranges trap, by catching Monochamus alternatus and the black longicorn of dragon spruce in a large number, and control pine nematode.
18. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 17 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Spacing between described trap is 20 ~ 70m, forest density or Disaster degree higher, the spacing of trap is less.
19. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 17 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
And use other means of prevention.
Claims (19)
1., based on monitoring and the preventing and controlling pine wilt disease method of catching in a large number of Monochamus alternatus and the black longicorn of dragon spruce, it is characterized in that,
Having the trap netted setting at regular intervals of luring with composition, described in lure with composition containing 2-(11 oxygen)-1-ethanol, australene and ethanol.
2. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Describedly lure with composition, by each trap containing 2-(11 the oxygen)-1-ethanol of 10 ~ 1000mg, the australene of 1 ~ 400g and the ethanol of 1 ~ 400g.
3. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described luring also comprises more than to be selected from (±)-3-carene, ipsenol, ipsdienol, n-pentacosane, n-nonacosane a certain with composition.
4. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 3 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described luring uses composition, by each trap containing (±)-3-carene 1 ~ 400g, ipsenol 10 ~ 3,000mg, ipsdienol 10 ~ 3,000mg, n-pentacosane 0.1 ~ 10mg, n-nonacosane 0.1 ~ 10mg.
5. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described luring is housed in macromolecule material pouch with composition, is arranged on the outside or inside of described trap.
6. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 5 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described macromolecule material is some in low density polyethylene (LDPE) (LDPF), high density polyethylene (HDPE) (HDPE), thermoplastic polyurethane (TPU).
7. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 6 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described macromolecule material pouch comprises inside and is filled with cotton polyethylene pipe, described in lure with in composition, australene and ethanol are filled in described internal pouch, and 2-(11 oxygen)-1-ethanol is filled in described polyethylene pipe.
8. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 1 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described trap is the vertical continuous funnel trap connecting 5 ~ 10 funnel traps and formed.
9. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 8 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described trap be connected with bottom catch Monochamus alternatus and the black longicorn of dragon spruce catch bucket, have lid topmost, the upper diameter of described funnel trap is 20cm ~ 30cm, and lower diameter is 7cm ~ 14cm.
10. the monitoring based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 9 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described bucket of catching comprises and inner anti-ly escapes with funnel, and described anti-to escape by the wall of funnel and the angle of funnel be less than 45 degree, and the length of funnel is more than 6cm.
11. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to any one in claim 1 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is following some a) in c), by monitoring the generation of Monochamus alternatus and dragon spruce black longicorn time normal, and control pine nematode:
Softwood forest in the area that a) pine nematode do not occur
B) softwood forest in the cleaning area of moving back pine nematode has been controlled
C) kainogenesis ground adjoining area or the area adjacent with disaster area in softwood forest.
12. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Interval between described trap, not occurring in area or clean area is 70 ~ 100m.
13. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Interval between described trap is 50 ~ 100m in described adjoining area.
14. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 11 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
By described generation monitoring, grasp the opportunity of suitably broadcasting sowing of ground control or aero control and be suitable for, control pine nematode.
15. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to any one in claim 1 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is kainogenesis ground or the small-scale disaster area of pine nematode, centered by dead and drying tree or disaster site, trap is set, and in the region set with horizontal 500m ~ 2km, longitudinal 500m ~ 2km, to be captured as object, netted trap is set, in described extra-regional 100 ~ 200m place, around arranging 10 ~ 100 traps for the purpose of monitoring, by catching in a large number and extra-regional 2 disaster-stricken diffusion monitoring in region, control pine nematode.
16. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 15 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Spacing between described trap is 50 ~ 100m, forest density or Disaster degree higher, the spacing of trap is less.
17. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to any one in claim 1 to 10 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Described controlling object area is the extensive disaster area of pine nematode, in the whole region in described disaster area, to be captured as object, nettedly arranges trap, by catching Monochamus alternatus and the black longicorn of dragon spruce in a large number, and control pine nematode.
18. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 17 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
Spacing between described trap is 20 ~ 70m, forest density or Disaster degree higher, the spacing of trap is less.
19. monitorings based on Monochamus alternatus and the black longicorn of dragon spruce according to claim 17 and the preventing and controlling pine wilt disease method of catching in a large number, is characterized in that,
And use other means of prevention.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2014-0107837 | 2014-08-19 | ||
| KR1020140107837A KR101530827B1 (en) | 2014-08-19 | 2014-08-19 | Control method of pine wood nematode by monitoring and mass-trapping of Monochamus aternatus and Monochamus saltuarius |
| PCT/KR2014/009049 WO2016027921A1 (en) | 2014-08-19 | 2014-09-26 | Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105530811A true CN105530811A (en) | 2016-04-27 |
| CN105530811B CN105530811B (en) | 2018-01-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201480048045.4A Active CN105530811B (en) | 2014-08-19 | 2014-09-26 | Method for preventing and treating pine wood nematode disease by pre-observing and largely capturing monochamus alternatus and monochamus spruce flower monochamus alternatus |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP6067194B2 (en) |
| KR (1) | KR101530827B1 (en) |
| CN (1) | CN105530811B (en) |
| TW (1) | TW201607428A (en) |
| WO (1) | WO2016027921A1 (en) |
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| CN106538607A (en) * | 2016-10-17 | 2017-03-29 | 浙江农林大学 | A kind of Monochamus alternatus attractant and its using method |
| CN107258773A (en) * | 2017-04-09 | 2017-10-20 | 华南农业大学 | A kind of screening technique of Monochamus alternatus infochemical slow-release material |
| CN107439282A (en) * | 2017-06-28 | 2017-12-08 | 唐喜军 | The anti-ease device of longicorn and its prevention and controls |
| CN109645003A (en) * | 2019-01-19 | 2019-04-19 | 杭州科森农化有限公司 | A kind of Monochamus alternatus Hope pheromone attractant |
| CN112042647A (en) * | 2020-09-15 | 2020-12-08 | 中国林业科学研究院亚热带林业研究所 | Attractant for adult anoplophora chinensis |
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| KR102705117B1 (en) * | 2021-09-06 | 2024-09-11 | 주식회사 케난 | Apparatus for catching insect vector of pine wilt disease |
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Also Published As
| Publication number | Publication date |
|---|---|
| JP2016531592A (en) | 2016-10-13 |
| TW201607428A (en) | 2016-03-01 |
| WO2016027921A1 (en) | 2016-02-25 |
| CN105530811B (en) | 2018-01-02 |
| KR101530827B1 (en) | 2015-06-24 |
| JP6067194B2 (en) | 2017-01-25 |
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