KR20210051249A - Control method of Pine wilt disease using mixture of attractant and insecticide for Pine wilt disease-mediating long-horned beetle - Google Patents

Abstract

The present invention relates to a method for controlling pine wilt diseases to spray both an attractant and an insecticide for pine wilt disease-mediating long-horned beetle. In the method of the present invention, by spraying an attractant for attracting pine wilt disease-mediating long-horned beetle together with an insecticide for killing insects against pine wilt disease-mediating long-horned beetle, it is possible to effectively control pine wilt diseases by killing insects mediating long-horned beetle induced by an excellent attractive force, which is the advantage of an attracting trap of the pine wilt disease-mediating long-horned beetle and increasing insecticidal efficiency of the insecticide.

Description

Control method of Pine wilt disease using mixture of attractant and insecticide for Pine wilt disease-mediating long-horned beetle}

The present invention relates to a method for controlling pine reindeer nematode disease, and more specifically, to a method of controlling pine reel nematode disease by using a mixture of an attractant that attracts sky cattle that mediates pine nematode disease and an effective insecticide thereto.

Pine wood nematode ( Bursaphelenchus xylophilus ) causes Pine Wilt Disease (pine wilt disease) on pine, sea pine, and pine trees. Pine tree nematode disease, also called pine tree AIDS, occurs when pine tree nematode invades pines. The invading pine tree nematode spreads and propagates in the water body while rapidly moving up and down through the resin map on the bark. When a physiological abnormality occurs in the water body, the number of nematodes increases rapidly, and the movement of moisture and nutrients is blocked and the pine tree dies. Pine nematode disease is a deadly disease in which infected pines invade from the 6th day, the leaves sag, and the leaves begin to wither on the 20th, and after 30 days, the leaves rapidly turn red and die 100%.

The pine tree nematode is an indigenous species in North America (Knowles et al., 1983; Dwinell, 1993), and most of the native species such as the United States, Canada, and Mexico are known to have resistance to the pine tree nematode (Kim et al., 2003). On the other hand, it is causing enormous damage to the species of other countries that do not have resistance.

In Japan, after the damage was confirmed in the early 1900s, the damage gradually spread, and now pines and sea pines are almost annihilated (Mamiya, 1988; Kishi, 1995).In China, pines, sea pines, and Mami pines were first discovered in 1982. In Taiwan, it is said that Yugusong, Haesong, and Taiwanese Lee Yeopsong are in danger of annihilation after their first discovery in 1985 in Taiwan (Enda, 1997).

In Korea, since it was discovered in Mt. Geumjeong, Busan in 1988, the damage has continued to spread, and it has expanded in up to 67 cities, counties, and gus. After a decrease, it increased to 50 cities, counties, and gus in 10 cities, provinces, and gus across the country in 2012, and the damage area peaked at 7,871ha in 2006 and decreased every year to 3,547ha until 2010, and then increased again in 2011 to 5,286ha in 2012. It was recorded (2012 annual report on the outbreak of forest parallel insects, National Institute of Forest Science). However, in 2013, the occurrence area increased by 119% compared to the previous year, reaching 11,550ha, the largest since the survey, and the outbreak areas were also found in 13 cities, provinces, and 59 cities, counties, and gus across the country. Existing areas of Gyeongbuk and Jeju were severely damaged, and new outbreaks were confirmed in Yangpyeong, Hanam, Yeoncheon, Gapyeong, Chungju, Chungju, and Gwangju Metropolitan City in Gyeonggi Province (2013 annual report on the outbreak of forest pest outbreaks, National Institute of Forest Science). In 2014, the incidence area decreased compared to the previous year, but the number of damages increased further. The number of new occurrences increased to Seongbuk-gu, Seoul, Icheon, Yeoju, Pyeongtaek, Uiwang, Gyeonggi, Jeongseon, Chungnam, Taean, Jeonbuk, Sunchang, Gimcheon and Yeongju, Gyeongbuk, and Yeongdo and Nam-gu, Busan.

The number of victims tested was 2.18 million copies from May 2013 to April 2014, 1.74 million copies by April 2015, 1.37 million copies by April 2016, 990,000 copies by April 2017, 690,000 copies by April 2018, and 2019. Until April, there was a trend of continuous decline to 490,000 copies. However, as of April 2019, the number of damages has been expanding to 117 cities, counties, and wards.

Pine nematode is a direct cause of disease, but its ability to move is very low, so it cannot move between trees and trees. What moves them is the sky-so-ryu, such as the brush-bearded sky cow and the northern bearded sky cow, which are symbiotics. These sky cows are called pine tree nematode disease medium sky cows, and methods for controlling them include a control method using a manned trap and a control method by spraying air (ground) insecticides.

Looking at the target sites listed in the current guidelines for controlling pine nematodes, aerial spraying of pesticides is applied to all areas where re-nematode disease has occurred, and water sources, residential areas, organic farming areas, residential areas, and areas where aircraft operation is difficult are excluded. Ground spraying of pesticides is carried out in the fleet and in areas where re-nematodes are concentrated, areas where control is omitted, areas where control of damaged dead trees is difficult, special protected areas, areas where aircraft operation is difficult, etc.

In the case of the vector insect attraction trap, the target site is where re-nematode disease occurs every year in areas where the damage is'extreme' or'deep', or where more than 30% of all pine trees have occurred, and where the occurrence site investigation is required. In areas where there is no occurrence, new areas, and fleet sites, the installation of traps for vector insects is restricted. However, in the administrative matters, it is recommended to conduct chemical spraying in the area where the vector insect inducement trap is installed.

Looking at the method of controlling pine tree nematodes in the guidelines for controlling pine tree nematodes, the control method by spraying pesticides is used as the main control method, and among them, aerial spray is the core, and areas where aerial spray is restricted are supplemented with ground spray. I'm using it. The control method using the vector insect attraction trap is used as a conditional control method only in areas with severe pine nematode disease.

The control method using the pine tree nematode disease medium flying cattle trap has a disadvantage in that there is a hassle to install and manage the trap in the mountain area, and the control method by spraying an insecticide has a problem that it is difficult to disperse the effect and confirm the effect. As such, both of these methods have advantages and disadvantages, but they are rarely used simultaneously even though it is recommended to use them with a greater emphasis on disadvantages rather than advantages.

Monochamus aternatus and Monochamus saltuarius, which mediate pine nematodes , take a variety of actions, such as migration, dispersion, feeding, and spawning after fable escape.These actions include pheromones released by vector insects and pines. It is deeply related to odors such as emanating volatile substances. In other words, there are core substances necessary for their respective roles in the dessert behavior, mating behavior, and living behavior, and the substances that have been identified currently include collective pheromones and various pyromones.

The control strategy of the'mediator trap' is to install traps and attractants (aggregate pheromones, pyromones) before the emergence of male and female adult insects, and catch them as an optimized vector trapping trap through the role of the attractant for each period or complex role. This starts from the fable of vector insects and proceeds throughout the entire period from the time of dessert, mating, and spawning.

Two conditions must be met for the movement and dispersion of vector insects. The first is food necessary for adult survival, and the second is a place for spawning. In order to find an environment that satisfies this condition, both male and female vectors rely heavily on the sense of the antennae.

Vector insects use their sense of smell to find pine trees for dessert. It senses the smell of natural volatile organic compounds produced and emanated from trees through the sense of smell. The main component of the substance is terpenes, especially monoterpenes with strong volatile and low molecular weight. Among the monoterpenes emanating from pines, α-pinene, β-pinene, 3-carene, camphene, myrcene, and limonene ( limonene) and others dominate. These substances are mainly used by pines as a defense material against insects and bacteria or as an allelopathy for the survival competition between plants, but many beetles recognize it as pyromones. Among them, α-pinene is the most contained and the emission is the highest. That is, it is judged that male and female vector insects mainly use α-pinene among various monoterpene-based volatile substances emitted from pine trees to find a dessert place. In the early stage, both male and female are attracted by judging them as hosts for dessert, and after dessert, they are continuously attracted due to feeding activities and mating behaviors for survival.

Ethanol is a substance that is produced and released when trees are under severe stress, and acts as pyromones in many beetles. For vector insects, the choice of a spawning site should be a feeding place for future safety and healthy growth. Pines with weakened water strength (physical damage) from which ethanol is emitted, or pines that are dying are selected as the spawning site. In other words, ethanol is a very important factor in selecting a spawning site. In addition, males and females who are not active but unable to mate are also attracted to this substance in the process of finding a mating location.

All behaviors of male and female vector insects are for survival, and survival is for later production, which is a common instinctive process and purpose of all insects. In particular, mating for later generation is an important reason for survival and a starting point for later generation. Although the collective pheromone of vector insects has not been identified as a direct element of the mating behavior, it is certain that it is a very important fundamental condition in the process of going to the mating behavior.

Collective pheromones are the core of attractants and play a number of functions. In other words, collective pheromone is an important factor in the capture following male and female attraction, and it gives us the selectivity to catch the pest we want and the selectivity to prevent unnecessary capture. It is a collective pheromone released by males, and has an attractive effect on both females and males. Due to this odor, it is captured by traps in the process of searching for a target, showing a very strong attraction.

Korean Patent Registration No. 10-0767783 Korean Patent Registration No. 10-1530827 Korean Patent Registration No. 10-1641927

The present invention removes the trap from the concept of the trapping trap of pine tree nematode disease, and changes the collective pheromone monochamol, which is the core attractant, to the spraying method. The purpose of this study is to provide a method of controlling pine tree nematode disease by saving the tree and increasing the insecticidal efficiency of the insecticide against the vector of pine tree nematode disease.

In order to achieve the above object, the present invention provides a method for controlling pine tree nematode disease, characterized in that spraying together with an attractant for pine tree nematode disease-borne skyworm and an insecticide for pine tree nematode disease-mediated skyworm.

In the above method, it is preferable that the attractant is monocamole.

In the above method, the attractant and the insecticide may be sprayed by mixing the attractant and the insecticide and spraying the same, or by applying the attractant first and then spraying the insecticide.

In the method of the present invention, by spraying a lure agent that attracts pine tree nematode disease medium sky cattle together with an insecticide that insects the pine tree nematode disease medium sky cattle as an insecticide, the medium sky cattle attracted by the excellent attraction force, which is the advantage of the pine tree nematode disease medium sky cattle, is the advantage of the trap. Insecticide increases the insecticidal efficiency of the insecticide, and can effectively control pine tree nematode disease.

Attractant and insecticide can be mixed and sprayed at the same time, or the attractant can be applied first and then pesticide can be applied. Both methods increase the insecticidal efficiency of the pine tree nematode disease vector by increasing the concentration of each flying cattle to the affected area by using the attractant. It can increase the control effect and reduce environmental pollution by reducing the amount of pesticide application.

FIG. 1 shows an embodiment of simultaneously treating an attractant and an insecticide in the method for controlling pine nematodes mediated sky cattle of the present invention.

The present invention relates to a method of controlling pine tree nematode disease by spraying together with an attractant for pine tree nematode disease and an insecticide.

Inducement agents for sky cattle that mediate pine tree nematode disease can use attracting substances that can attract sky cattle that mediate pine tree nematode disease.

As the attractant, it is preferable to use monocamole, which is a medium-sized sky cattle attractant prepared for spraying. Monocamole is 2-(Undecyloxy)-1-ethanol {2-(Undecyloxy)-1-ethanol)}, a type of collective pheromone released by males of the genus Monochamus. It has a very strong attraction to both males and females of and Northern Bearded Sky Cow.

The attractant may further include at least one selected from α-pinene and ethanol.

α-Pinene is a substance that enables the sexual immature individuals of the brush bearded sky cattle and the northern bearded sky cattle and the female and male of the sexually mature individuals to determine the location of the host (pine, etc.), and the sexual immaturity of the brush beard sky cattle and the northern bearded sky cattle. And in both males and females of sexually mature individuals. Even if they emerge as adults, they cannot mate unless they are sexually mature. After becoming an adult, they must go through a period of sexual maturity in order to be able to mate, and during this period, they are transformed into sexually mature individuals through the process of feeding and persecution (dessert). At this time, immature individuals who escaped the fable detect substances such as α-pinene by finding the shoots of pine trees.

Ethanol is released when a plant is subjected to stress (physical damage, dead or dead), and an individual who recognizes this is attracted. In particular, it is highly active from the beginning on sexually mature individuals, and influences the spread of α-pinene, (±)-3-karene, leaf senol and ipsedienol so that more individuals can recognize it.

The attractant is preferably mixed with an insect pheromone organic solvent, and physically stirred at room temperature and pressure, and mixed.

As an insecticide, an insecticide that has an insecticidal effect on the vector of pine nematode disease can be used. The representative pine tree nematode disease medium sky cattle are the brush beard sky cattle and the northern beard sky cattle. It is preferable to use a pesticide for air spraying as the pesticide.

At the same time, the attractant and the pesticide can be mixed and applied to the affected area, or the attractant may be applied to the affected area first, and then the pesticide can be sprayed at the concentration point when the vector sky cattle are concentrated to the affected area by the attractant. It is sprayed by the method of air spraying or ground spraying, and it is more preferable to spray by air. At this time, concentration refers to the two situations where the medium sky cattle gather to the manned treatment area and the medium sky cattle do not leave the treatment area.

In both methods, by increasing the concentration of flying cows to the affected area by an incentive agent, it is possible to increase the control effect by increasing the insecticidal efficiency of the flying cows, and reduce the amount of pesticide sprayed to reduce environmental pollution.

When using a mixture of attractants and pesticides, it is preferable to mix them by physically stirring them at room temperature and pressure.

Fig. 1 shows an example in which an attractant and an insecticide are mixed and treated at the same time in the method for controlling pine tree nematode disease of the present invention.

Monocamole is mixed with an insect pheromone organic solvent, and physically stirred and mixed at room temperature and pressure.

The organic solution in which the monocamole is dissolved is mixed with an insecticide for controlling and spraying pine worms nematode by physically stirring at room temperature and pressure.

The mixture of attractants and pesticides is checked for chemical modification and sprayed by air or on the ground.

After spraying, the insecticidal effect and efficiency are checked by installing a monitoring vector insect inducement trap.

Claims (3)

A method for controlling pine tree nematode disease, characterized in that spraying together with an attractant for pine tree nematode disease and an insecticide for pine tree nematode disease. The method of claim 1,
The method of controlling pine nematode disease, characterized in that the attractant is monochamol. The method according to claim 1 or 2,
The attractant and the insecticide are sprayed by mixing the attractant and the insecticide at the same time, or by spraying the attractant first and then spraying the insecticide.

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