KR101044245B1 - Composition for attractants of Platypus koryoensis - Google Patents

Abstract

The present invention relates to a Gwangneung gingko bite attractant composition, and more particularly, selected from the group of nerol, neral, geraniol, geranial, citronellol Gwangneung Ginger Root Attractant Composition comprising any one or more components, and more preferably, Neol, neral, and geraniol isolated from sawdust generated during oak drilling The present invention relates to a Gwangneung-Ginwooksome attractant composition comprising any one or more components selected from the group of geranial and citronol.

Gwangneung long tree, attractant, nerol, neral, geraniol, geranial, citronellol

Description

Composition for attractants of Platypus koryoensis

The present invention relates to a Gwangneung gingko bite attractant composition, and more particularly, selected from the group of nerol, neral, geraniol, geranial, citronellol Gwangneung Ginger Root Attractant Composition comprising any one or more components, and more preferably, Neol, neral, and geraniol isolated from sawdust generated during oak drilling The present invention relates to a Gwangneung-Ginwooksome attractant composition comprising any one or more components selected from the group of geranial and citronol.

The deciduous forests, which account for 26% of Korea's forests, have recently gained increasing attention as their importance in regards to revaluation of economic value and eco-friendly management of forests.

There are various kinds of deciduous forests in Korea according to species. Especially, among the deciduous forests, the oak species suitable for the location of Korea occupy about 27% of the total tree stock accumulation in Korea. It is widely used for forest resources such as cultivation of shiitake as well as materials for building, construction materials and charcoal.

However, in late August 2004, after the oak wilted disease was first discovered in "Lee Bae Jae" in Seongnam-si, Gyeonggi-do, damage was found nationwide (see Fig. 1). In the case of Gyeonggi-do, which has been the most damaging, the number of dead trees has increased since 2004, but the number of dead trees in 2008 was 4,112, down from 5,230 in 2007. .

The platypus koryoensis, which is known to spread oak rot pathogens, is an insect of the coleopteran long tree, which is inhabited by oleander, larch, oak, and roe trees. (木質部) is added to the core material (心 材) to eat into the weak wood quality. This Gwangneung long tree is distributed in the Far East such as Korea, Northeast of China and Russia. It was first reported in 1935 in Korea. Adults are dark brown with average length of 4.4mm for females and 4.2mm for males. The female's back has mycangia, which carries the pathogen. When a female enters the tunnel, the fungus in the fungus spreads and spreads in the tree, and the young worms (larvae) awakening from the eggs grow on the pathogen fungus or yeast cultured in the tree tissue.

Currently, the control of oak wilting disease in Korea is mainly fumigation with methamsodium liquid after logging the infected oak. The effectiveness of organic synthetic pesticides has been excellent, but the use of pesticides has led to problems such as reduced pest natural enemies, resistance development, the development of latent pests, and toxicity to humans and livestock.

Therefore, a new environmentally friendly control method is very necessary, and the development of attractants using pheromone is an environmentally friendly control method and is considered as an alternative to replace organic synthetic pesticides that cause many social problems due to side effects. Much research has been conducted with interest in insect pheromones as a commercial attractant development or leading compound.

Pheromone is defined as a physiologically active substance which is discharged from an animal subject to the outside of the body and causes a specific reaction such as a certain behavior or a physiological reaction to another individual of the same kind. Recently, insect pheromone analysis can be performed in a small amount due to the development of analytical techniques, and these pheromones have been used in the field for pest prediction and scattering disturbance. In particular, long-habited tree and insects such as Gwangneung long-tree is known to attack host plants by secreting Aggregation pheromone, can be used as an effective control method for the development of attractants using pheromone.

However, there are no studies on the development and use of attractants and traps using aggregated pheromones isolated from Gwangneung long tree.

An object of the present invention is to provide a Gwangneung long tree bite attractant composition that can be removed by attracting Gwangneung long tree bite to prevent oak wilting disease.

The present invention is any one or more components selected from the group of nerol (nerol), neral (geral), geraniol (geraniol), geranial (geranial), citronellol, more preferably male gwangneung Glutinous and / or uncoated Gwangneung Ginger Root Males are attracted to Gwangneung Ginger Romsome containing any one or more components selected from the group of nerol, neral, geraniol, geranial and citronolol isolated from sawdust generated during oak drilling. An agent composition is provided.

By the Gwangneung long tree bite attractant composition of the present invention can attract and remove the Gwangneung long tree bite propagating pathogens of oak wilting disease can prevent and / or control the oak wilting disease occurring in the oak.

The present invention represents a Gwangneung long tree bite attractant composition.

The present invention is a gwangneung long containing any one or more components selected from the group of nerol, neral, geraniol, geranial, citronellol of the following structural formula To provide a bark attractant composition.

......네롤(Nerol)

...... Nerol

...... 네랄(Neral)

...... Neral

..... 게라니올(Geraniol)

..... Geraniol

...... 게라니알(Geranial)

Geranial

......시트로네롤(Citronellol)

Citronellol

The present invention occurs when one or more components selected from the group of nerol, neral, geraniol, geranial, citronerole, more preferably male gneungneum geungeot male male carcass and / or male ginseng geunneung beetle male perforated oak It is to provide a gwangneung ginkosome attractant composition comprising any one or more components selected from the group of nerol, neral, geraniol, geranial, citronolol separated from the sawdust.

The pulverized male carcass of Gwangneung in the above is pulverized and then put into an organic solvent and extracted to separate components of nerol, neral, geraniol and geranial.

In the above, alum ginseng can be separated from the components of nerol, neral, geraniol, geranial and citronol by extracting sawdust generated during drilling of oak in an organic solvent.

The organic solvent is hexane, pentane, diethyl ether, acetone, acetone, DMSO (dimethylsulfoxide), dichloromethane0, chloroform, chloroform, methanol ), Ethanol can be used.

When extracting male carcasses of Gwangneung long-tree worms, the temperature can be extracted for 1 hour under normal temperature and 1 atm. When extracting the ingredients of Ranial, Citronerole can be extracted for 24 hours at room temperature, pressure under 1 atm.

The present invention provides a Gwangneung ginko tree attractant composition wherein citronol: nerol: neral: geraniol: geranial is mixed in a weight ratio of 1-3: 1-3: 1-4: 1-3: 1-5. Indicates.

The present invention shows a Gwangneung-Ginwook attractant composition in which citronerol: nerol: neral: geraniol: geranial is mixed in a weight ratio of 1: 1: 2: 1: 5.

Gwangneung gingko yeast attractant composition comprising any one or more components selected from the group of nerol, neral, geraniol, geranial, citronerole of the present invention is a granule, powder, smokescreen, capsule, gel (gel) It can be formulated in any one or more formulations selected from the group).

The present invention includes an attractant trap containing the above-mentioned Gwangreung gingko bite attractant composition.

By using the Gwangneung long tree bite attractant composition of the present invention can be attracted, removed Gwangneung long tree bite causing the oak thread. At this time, under the trap of one or more funnel-shaped traps Gwangneung long-tree ginseng composition containing the Gwangneung long-tree ginseng composition to contain the container Gwangneung-long tree gimyeo by the Gwangneung long-tree gum attractant through the funnel-shaped trap By attracting to the capture container can remove the Gwangneung long tree.

The funnel-shaped trap may be a stack of a plurality of funnel-shaped traps according to the size of the tree is installed funnel-shaped trap.

The Gwangneung long-tree ginseng capping container may be removed by putting the water or a mixture of water and ethanol in addition to the Gwangneung long-tree ginseng attractant composition to remove the gwangneung long-tree ginseng attracted to the container.

The Gwangneung gingko moth attractant composition of the present invention was investigated under various conditions. In order to achieve the object of the present invention, it is preferable to provide the Gwangneung gingko moth attractant composition under the above-mentioned conditions.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Gwangneung long tree bitumen male and female extracts and extracts

In the oak forest of Paju, Gyeonggi-do, the locust tree (Quercus monglica) infected by Gwangneung long tree was collected. The cross section of the wood chips of the gingko tree was sealed with fly pins to maintain moisture. These chrysanthemums were placed in a plastic container containing wet moss in a room at 25 ° C. and stored under a condition of 16 hours: 8 hours. Adults were pulled out of the timber of the tree bark every day, and sex was used for the experiment.

In order to search for sex-specific material of the Gwangneung long-tree tree tree collected from the gingko tree, 100 adult male and female carcasses were put into hexane and homogenized. The hexane solution was filtered through glass wool, n-decanol was added, and then concentrated using nitrogen. At this time, in order to identify the pheromone storage site of Gwangneung long tree, the adult male and female body was separated into head, chest, and pear and extracted with hexane.

As a result of analyzing the extract of the male and female of Gwangneung long-tree, male and female, GC-FID, it can be seen that there is a compound unique to males (see peaks a to d of FIG. 2). These materials were analyzed by GC-MS analysis and comparison of retention index, peak a of FIG. 2 is a nerol of the following structural formula, peak b of FIG. 2 is a neural of the following structural formula, and FIG. Peak c of 2 was identified as geraniol of the following structural formula, and peak d of FIG. 2 was identified as geranial of the following structural formula.

In FIG. 2, FIG. 2A is a graph showing the results of analysis of the worm extract of male and female Gwangneung long-tree ginseng by GC-FID, and FIG. 2B shows an enlarged portion of peaks a to d of FIG. 2A.

......Nerol,

......Neral

...... Nerol,

...... Neral

.....Geraniol,

......Geranial

..... Geraniol,

Geranial

On the other hand, the amount of each compound present in the male and female of the Gwangneung Ginyeongdo adult adults is shown in Table 1.

Analysis of extracts of the head, thorax, and abdomen of adult females of Gwangneung long-necked tree showed that nerol, neral, geraniol and geranial substances were detected only in extracts of pears. In the extract of the chest area of male Gwangneung-Gin tree, males were only slightly detected (see FIG. 3). In FIG. 3, Internal Standard (IS) is a compound used to measure the amount of each extract using GC as an internal standard, and IS in the graph of FIG. 3 is decaneol (n-decanol, 10 ng).

Table 2 shows the amount of each compound in the pear extracts of male adult females of Kwangreung-Gin. The ratio of four components of nerol, neral, geraniol and geranial in the extract of Gwangneung long-tree bark was 3: 38: 5: 54 (nerol: neral: geraniol: geranial). The ratio of the four components was 1: 37: 2: 61 (nerol: neral: geraniol: geranial).

Table 1 Contents of nerol, neral, geraniol and geranial in extracts of male and female extracts

castle
Compound Amount (mean ± standard deviation, ng / beetle, N = 8) Nerol Neral Geraniol Geranial cock 1.85 ± 0.64 (OF ^* = 7/8) 22.29 ± 5.86 (OF = 8/8) 3.07 ± 0.86 (OF = 8/8) 32.19 ± 9.03 (OF = 8/8) female 0.54 ± 0.28 (OF = 3/8) 0.12 ± 0.10 (OF = 2/8) 1.40 ± 0.34 (OF = 7/8) 0.21 ± 0.14 (OF = 2/8)

^* = OF occurrence frequency

<Table 2> Contents of Nerol, Neral, Geraniol and Geranial by Male Adults

Body part
Compound Amount (mean ± standard deviation, ng / beetle, N = 5) Nerol Neral Geraniol Geranial head - - - - -chest - 1.59 ± 1.59 - - ship 0.55 ± 0.23 26.62 ± 3.52 1.12 ± 0.87 44.16 ± 5.58

Example 2 Collection of Volatile Materials in Perforated Sawdust

Soak the wood chips (15cm long, 15-20mm in diameter) of the tree that has not been attacked by the Gwangneung long-tree bark in water for 17 days, and then remove the water and artificially invade the holes using a power drill. 60 pieces of 2mm, 1.75 ± 0.25cm) were made 5cm apart. Adult males of Gwangneung long-tree spp. Were introduced into each infiltration hole using a plastic micropipette chip. Males of Gwangneung long tree piled a bird-shaped perforated sawdust outside the invasion hole within one day. Five days after the introduction of male Gwangneung long-trees, males collected perforated sawdust accumulated by males of Gwangneung-long-trees using tweezers and soaked in hexane for 1 day.

The male and female of the Gwangneung long-tree were introduced to the infestation hole where male Gwangneung-long tree was removed. If the Gwangneung long-tree jocks succeeded in mating, the shape of the perforated sawdust piled up outside the invading hole changed into a ball shape, and it was judged whether the Gwangneung long-tree bosom mated. Five days after the introduction of females of Gwangneung long-tree, the perforated sawdust accumulated in the infiltration hole was collected and soaked in hexane for 1 day.

As a control, a sawdust of wood chips inoculated with Gwangneung long tree was made using a power drill, and then soaked in hexane. Each hexane solution was added to an internal standard, concentrated with nitrogen, and used for analysis.

When male adult males of Gwangneung long-tree were introduced into artificial invasion holes, galleries were formed in the invasion holes of 72.4%, and average of 6.4mg of perforated sawdust was produced per invasion hole. After that, when the females were mated with Gwangneung Ginger, some 14.8 mg of boring sawdust was discharged. The extracts of perforated sawdust extracted by male Gwangneung gingko males were analyzed by GC-FID and GC-MS.The extracts contained four components, nerol, neral, geraniol and geranial, Together citronellol of the structure

......시트로네롤(Citronellol)

Citronellol

On the other hand, the above components were not detected in the perforated sawdust and male control perforated sawdust discharged after mating male and female of Gwangneung long tree.

Citronellol was not separated from nerol in DB-1MS columns, but citronellol and citronellol and nerol in DB-FFAP columns and DB-5MS columns. The ratio of was 1: 1. The ratio of each component was 22: 4: 18: 55 (the order of citronellol + nerol / neral / geraniol / geranial).

The amount of each compound in the perforated sawdust extract is summarized in Table 3.

<Table 3> Content of aggregated pheromone in perforated sawdust

Sawdust type
Iterations
Compound Amount (Mean ± Standard Deviation, ng / beetle) Nerol + citronellol Neral Geraniol Geranial Sawdust produced by uncooked males 3 18.33 ± 2.69 3.48 ± 1.00 14.93 ± 7.34 44.93 ± 5.76 Sawdust produced by mating females and males 3 - - 0.78 ± 0.78 1.01 ± 1.01 Control group 3 - - - -

Example 3 Gas Chromatography (GC) and GC-Mass Spectrometry (GC-MS)

Gas chromatography (GC) analysis used in the compound analysis in Examples 1 and 2 was performed by using a hydrogen ionization detector (FID) Agilent 6890N gas chromatograph, DB-1MS and DB-FFAP (30m × 0.25) mm id, 0.25 μm film thickness (J & W Scientific, Folsom, Calif.) columns were separated and analyzed. The oven temperature conditions were as follows: After holding at 40 ° C. for 1 minute, the temperature was increased to 250 ° C. at a rate of 6 ° C. per minute, and then maintained at that temperature for 4 minutes. As a carrier gas, helium (He) gas was used at a flow rate of 1.5 mL / min.

GC-mass spectrometry of the extracts was performed with DB-5MS (30m × 0.25mm i.d., 0.25µm film thickness; J & W Scientific) using 5973N MSD connected to Agilent 6890N gas chromatograph. Analytical conditions were the same as those of GC.

Example 4 Daily Changes in Aggregated Pheromones Produced by Males

The following experiments were conducted to measure the daily changes of volatiles, nerol, neral, geraniol, geranial and citronol, produced by male Gwangneung long-tree. As described above, after inoculating male buckwheat ginseng tree with male coriander, the wood chips were placed in a three-necked separation flask. A glass column filled with Super Q (Alltech, Deerfield, IL) (hereinafter referred to as a SuperQ column) was installed in the flask to capture the volatiles produced by male Gwangneung long-trees. Another Super Q column was used to purify purified air. Installed to feed. After the superQ column for collection was connected to the air pump, the air in the flask was aspirated at a flow rate of 300 mL per minute. The SuperQ column was exchanged daily, and the volatiles collected on the SuperQ column were separated using hexane. An internal standard (n-decanol) was added to this hexane solution, and then concentrated and used for analysis.

The daily change of the five components of nerol, neral, geraniol, geranial and citronol in the perforated sawdust discharged by the male Gwangneung long tree is shown in FIG.

The production of five components lasted for 10 days, and the amount of geranial reached its peak on the second day after the male was introduced into the invasion hole, and the other components reached on the third day. After day 4, the emissions of all components decreased. The daily emissions and ratios of each ingredient average 50: 64: 44: 143 per animal (nerol: citronellol: neral: geraniol: geranial) (ng / adult) and 16: 22: 12: 51 (%) (nerol: citronellol: neral: geraniol: geranial).

Example 5 Tactile Conduction (EAG) Activity

A mixture component (10 μl) of each of pheromone, nerol, neral, geraniol, geranial and citronolol, extracted from perforated sawdust discharged from male Gwangneung long-trees, was applied to pieces of filter paper (50 × 5 mm). This piece of filter paper was placed in a Paster pipette. The silver wire was put into the glass tube filled with 0.1N KCl aqueous solution, and was used as an electrode. The cut surface of the cut head of the Gwangneung long-tree was contacted with the reference electrode, and the tip of the antennae was contacted with the recording electrode. A 0.5 second pulse stimulus was added to the air stream at 30 second intervals while continuously feeding the sample with a humid air stream (1.5 mL / min) through charcoal and water. The tactile potential signal was amplified by an amplifier (Combi EAG probe, Synthec) and recorded on a computer through a signal interface device (IDAC-2, Synthec). The order of the tactile stimulants was air, the components of the pheromone mixture (5 types), the air, and the stimulation order of the pheromone mixture components was random. The recorded EAG activity was normalized to% based on the amplitude of the response to air (100%).

The tactile sensations of both Gwangneung Ginseng and Male and females responded to the above five components and showed relatively high EAG activity (see FIG. 5). The neral showed the highest activity in the male tactile sense, and the activities of the four components except citronellol were higher in the female tactile than the male tactile sense. Citronellol had the lowest activity in the tactile sense of the male and female.

Example 6 Field Experiment

Field experiments were conducted in the oak forest of Paju, Gyeonggi-do on 7-28 May 2008. Two multiple funnel traps consisting of 20 clear plastic funnels (up to 9 cm in diameter and down to 2.5 cm in diameter) were installed on a single Singal tree. The bottom of the trap was placed 20cm above the ground. One of the two traps was installed on the bottom of the slope of the mountain and the other on the top of the slope. The plastic cup filled with water was fixed and installed under the bottom trap to catch the manned Gwangneung long tree. A mixture of 15 mg of pheromone dissolved in hexane, nerol, neral, geraniol, geranial and citronerol, was applied to cotton wool in the rubber septum, and the rubber septum was suspended in a trap 50 cm above the ground. The pheromone compound ratio was 1: 1: 2: 1: 5 (citronellol: nerol: neral: graniol: geranial). The traps had two rows of eight trees in one row (one block), each trap having a distance of 10m and a distance of 30m. In one block, a trap treated with a pheromone mixture and an untreated trap were alternately installed. A new attractant material, the pheromone mixture, was installed between 10-12 am in the morning. Caught insects were collected weekly, at which time the attractant was exchanged. Caught insects were separated by sex and captured. In order to confirm the positional effect, each trap was captured without pheromone treatment, and then the number of captures was compared.

In the above structure and installation method of the transparent multi-funnel trap was installed as shown in FIG. First, make a hole in both sides of a funnel-shaped transparent plastic container (8-12 cm in diameter and 7-10 cm in height), and connect 15 to 20 with a string. The bottom funnel was connected to a collection vessel (500-1500 ml) and the bottom of the collection vessel was filled with a mixture of water and ethanol.

Table 4 shows the results of capturing Gwangneung long tree in the outdoor trap test. The number of males captured in the trap treated with the pheromone mixture was significantly higher than that in the untreated trap. Female catches were significantly higher in pheromone-treated traps than in untreated traps, except for the second week. The number of traps in each trap without pheromone treatment was not significantly different.

<Table 4> Outdoor Pulmonary Effect

term

cock female Treatment Control Treatment Control N = 16 N = 16 N = 16 N = 16 May 7-May 14 5.5 ± 2.1 1.0 ± 0.8 1.4 ± 0.5 0.3 ± 0.2 May 14-May 21 9.2 ± 4.9 0.6 ± 0.3 1.1 ± 0.5 0.3 ± 0.3 May 21-May 28 68.4 ± 36.6 5.9 ± 3.3 44.4 ± 24.6 2.0 ± 1.2

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated that it can be changed.

The present invention can be removed by attracting the Gwangneung long tree bite composition to prevent oak wilting disease.

The present invention is a new environmentally friendly method for removing Gwangneung gingidae, which is a mediator of oak wilting disease, and can replace the use of organic synthetic pesticides that cause many social problems due to current side effects. Can contribute.

Figure 1 is a gallung tree that suffered from oak wilted by the Gwangneung long tree bite.

Figure 2 is an analysis of the Gwangneung Ginyeokdo female and male abdominal extract using gas chromatography.

Figure 3 is analyzed by using gas chromatography to extract the Gwangneung long-tree ginseng male and female extracts.

Figure 4 is the daily change amount of Gwangneung long tree bite pheromone.

5 is an electrophysiological response to the aggregated pheromone of the Gwangreung long tree antenna antenna.

FIG. 6 is a funnel trap structure used in the attraction test and outdoor attraction test of Gwangneung long-tree fungus pheromone outdoor.

Claims (7)

Gwangneung ginko tree attractant composition comprising any one or more components selected from the group of nerol, neral, geraniol, geranial, citronellol. The method of claim 1, wherein any one or more components selected from the group of nerol, neral, geraniol, geranial, and citronol are characterized in that the alum of the coriander Gwangneung male ginseng male is separated from the sawdust generated during oak perforation. Gwangneung long tree bite attractant composition. The method of claim 1, wherein the at least one component selected from the group of nerol, neral, geraniol, geranial is isolated from the Gwangreung gingiva male male cartilage. The method according to claim 1, wherein the Gwangneung Ginger Beet attractant composition includes all components of nerol, neral, geraniol, geranial and citronol. Citronol: nerol: neral: geraniol: geranial 1 to 3: 1 to 3: 1 to 4: 1 to 3: 1 to 5 in the weight ratio of the mixture of Gwangneung long-tree ginseng attractant composition. The method according to claim 1, wherein the Gwangneung Ginger Beet attractant composition includes all components of nerol, neral, geraniol, geranial and citronol. Citronol: nerol: neral: geraniol: geranial Gwangneung long tree bite attractant composition, characterized in that mixed in a weight ratio of 1: 1: 2: 1: 5. The method of claim 1, wherein the Gwangreung gingko bite attractant is formulated in one or more formulations selected from the group consisting of granules, powders, smokescreens, capsules, and gels. Claims 1 to 6, Gwangreung gingko bite attractant trap containing the Gwangreung gingko bite attractant composition of any one of claims 1 to 6.

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