CN103563896A - Monochamus alternatus hope attractant and application thereof - Google Patents

Monochamus alternatus hope attractant and application thereof Download PDF

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Publication number
CN103563896A
CN103563896A CN201310488407.9A CN201310488407A CN103563896A CN 103563896 A CN103563896 A CN 103563896A CN 201310488407 A CN201310488407 A CN 201310488407A CN 103563896 A CN103563896 A CN 103563896A
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attractant
monochamus alternatus
ethanol
alternatus hope
trapper
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CN103563896B (en
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邓建宇
王义平
王富民
唐爱妙
崔艮中
马四国
刘翠微
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Zoje Sifang Biotechnology Co ltd
Zhejiang A&F University ZAFU
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PHEROBIO TECHNOLOGY Co Ltd
Zhejiang A&F University ZAFU
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Abstract

The invention discloses a monochamus alternatus hope attractant and an application thereof. The monochamus alternatus hope attractant comprises a plant source attractant and aggregation pheromone 2-undecyl-1-ethanol, wherein the plant source attractant comprises the following components in percentage by volume: 60-85% of alpha-pinene, 0-20% of beta-pinene and 15-40% of ethanol; the dosage of the aggregation pheromone 2-undecyl-1-ethanol in each lure is 0.5-100mg. The novel compound attractant has the advantages of simple production process, low cost, ideal trapping effect, safety, environment friendliness and the like, and can be used for effectively controlling the inter-forest insect density of monochamus alternatus hopes and inhibiting the spread and propagation of pine wilt disease.

Description

A kind of Monochamus alternatus Hope attractant and application thereof
Technical field
The present invention relates to a kind of Monochamus alternatus Hope (Monochamus alternatus Hope) imago attractant of propagating pine wood nematode, belong to field of pest control.
Background technology
Pine wood nematode (Bursaphelenchus xylophilus) is to cause pine tree destructive disease---the pathogen of pine nematode.China finds that there is pine nematode in Nanjing first in nineteen eighty-two, in twenties short Nian Zhong ,Ci Bing China, expands and spreads rapidly, has expanded at present national 110Duo Ge counties and cities, causes the heavy damage of pine forest resource, and economic loss is huge.The generation of pine nematode and diffusion and host plant, vector insect are closely related.The most effective approach of controlling at present pine nematode is to control the occurrence and harm of vector insect, to reach the object of controlling pine nematode.The insect that occurring in nature can carry pine wood nematode has multiple, but as 13 kinds of the reports of vector insect, be all black sky Bos kind.The crucial vector insect of China is Monochamus alternatus Hope (Monochamus al ternatus Hope).Monochamus alternatus Hope is also in forestry, to endanger serious tree borer.Generation also can cause pine wilt disease lethal separately.Exploitation inducing substance, utilizes attractant trapping Monochamus alternatus Hope and the pathogenic nematode that carries is a kind of effectively preventing measure.It can round-the-clock monitoring and controls Monochamus alternatus Hope, realizes the control to pine nematode.
The Main Ingredients and Appearance of the attractant of the developments such as Chen Jiwen (application number 200810028389) is pure natural extract from pine needles or host tree phloem ethanol extract and ethanol.This attractant has crucial active principle and the mechanism of action is unclear, processing technology is complicated, production cost is too high, simultaneously will be by the needle of host plant or bark lixiviate, and cut down and consume a large amount of pine trees.
The moth rod type destructive insect attractant of the developments such as Zhao Jinnian (patent No. ZL03115289) is mainly formed by australene, nopinene, acetaldehyde, acetone, alcohol, and wherein the percentage by weight of various compositions is: australene 30~70%, nopinene 2~25%, acetaldehyde 2~20%, acetone 8~38%, alcohol 10~40%.
The component of the Monochamus alternatus Adults attractant of the developments such as Xiao Chun (application number 200910094345.7) with and percent by volume be: turpentine oil 60~80%, ethanol 16~24%, benzyl carbinol 2~10%, terpineol 2~6%.The percent by volume of each composition of Monochamus alternatus Adults attractant is: australene 24.64~32.86%, nopinene 22.91~30.55%, 3-carene 6.51~8.68%, β-phellandrene 2.97~3.96%, terpinolene 0.74~0.99%, laurene 0.62~0.83%, other 1.60~2.13%, ethanol 16~24%, benzyl carbinol 2~10%, terpineol 2~6%.
All there is the shortcomings such as composition is many, processing technology is complicated, production cost is high, attractant effect is undesirable in these attractants.Therefore, how to obtain can composition simply, the Monochamus alternatus Hope attractant that attractant effect is good be those skilled in the art's technical problem urgently to be resolved hurrily.
Summary of the invention
For the problems referred to above that exist in prior art situation, the Monochamus alternatus Hope attractant of the advantage such as the object of the invention is to that design provides a kind of and has that production technology is simple, with low cost, safety, environmental protection, allure are strong, population quantity for monitoring Monochamus alternatus Adults, effectively reduce insect density, the diffusion naturally of containment pine nematode is climing.In order to realize object of the present invention, intend adopting following technical scheme:
One aspect of the present invention relates to a kind of Monochamus alternatus Hope attractant, it is characterized in that described Monochamus alternatus Hope attractant comprises: botanical attractant and aggregation pheromone 2-hendecane Oxy-1-ethanol, wherein botanical attractant comprises the component of percent by volume australene 60%~85%, nopinene 0~20%, ethanol 15%~40%, and the content of aggregation pheromone 2-hendecane Oxy-1-ethanol is that 0.5-100mg/ lures core.
In a preferred embodiment of the present invention, the w/v of described nopinene is 1~20%, preferably 2~6%.
In another preferred embodiment of the present invention, described brown longicorn beetle attractant does not comprise other active component.
The present invention relates to a kind of board-like funnel type trapper that hits on the other hand, and wherein said botanical attractant is arranged in the core that lures that hits board-like funnel type trapper, and aggregation pheromone is arranged in rubber stopper.
The application that the present invention also relates to above-mentioned attractant or hits board-like funnel type trapper on the other hand, described is applied as for traping Monochamus alternatus Hope bisexuality adult and/or control pine nematode.
In a preferred embodiment of the present invention, described trapper hangs on the generating region of pine nematode and Monochamus alternatus Hope.
Useful result of the present invention is: 1, this attractant all has remarkable attractant effect to Monochamus alternatus Hope bisexuality adult, can be used for the generation in Monochamus alternatus Hope field and the prediction of causing harm, and also can be directly used in control, reduces field population.2, use cost is cheap, and production technology is simple.
Embodiment
Embodiment 1:
Now, in conjunction with the embodiment of the present invention, the invention will be further described, and further illustrate beneficial effect of the present invention by corresponding test.
Table 1 reagent source and purity
1 australene (64ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (0.5mg) of filling a prescription
2 australenes (64ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (5mg) of filling a prescription
3 australenes (64ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (10mg) of filling a prescription
4 australenes (64ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (20mg) of filling a prescription
5 australenes (61ml)+nopinene (the 3ml)+absolute ethyl alcohol (16ml) of filling a prescription
6 australenes (61ml)+nopinene (3ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (20mg) of filling a prescription
7 australenes (58.2ml)+nopinene (5.8ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (20mg) of filling a prescription
8 australenes (53.3ml)+nopinene (10.7ml)+absolute ethyl alcohol (the 16ml)+2-hendecane Oxy-1-ethanol (20mg) of filling a prescription
Contrast 1 australene (64ml)+absolute ethyl alcohol (16ml) (patent formulation that applicant applied in the past, application number: 201210557269.0)
Contrast the 2 product M-99 of Zhejiang gloomy inspection office
Prepare by a certain percentage botanical attractant, then botanical attractant 80ml is joined to the plastics slowly-releasing bottle (Taiyuan Xiaodian Area San Cheng plastic molding and processing plant) of a kind of 200ml.Monochamus alternatus aggregation pheromone 2-hendecane Oxy-1-ethanol is mixed with to n-hexane or the ethanolic solution that concentration is 0.5mg-100mg/100 microlitre.According to dosage, join and in rubber stopper, make aggregation pheromone and lure core.Monochamus alternatus aggregation pheromone lures core and botanical attractant to be used in combination together with trapper.Trapper is selected the board-like funnel type trapper that hits of Xuanzhou, Anhui Province production.
Different formulations and contrast be the attractant effect to Monochamus alternatus Hope at woodland.
Trapper is selected the board-like funnel type trapper that hits of Xuanzhou, Anhui Province production.Trapper is made by horse spoken parts in an opera iron sheet, top is the umbrella hood of footpath, end 41cm, the lower 4 blocks of gear worm plates for long 50cm, wide 13cm of cover, the middle top of plate installs one for laying the cylindrical core retainer plate (diameter 10cm, high 16cm) that lures that lures core, and jacket wall is covered with the circular hole of diameter 0.14cm; Gear worm plate below connects the funnel of upper bore 41cm, lower bore 9cm, funnel neck length 12cm, and hopper walls becomes 45 ℃ of angles with vertical plane.During trapping, the filler neck plastic sack of trapping, the insect arriving for collecting trapping.The core that lures of botanical attractant is the 200ml plastic bottle (Taiyuan Xiaodian Area San Cheng plastic molding and processing plant) of tool slow release effect.In plastic bottle, add attractant, test site is in peach blossom forest farm, At Meishan, changxing, zhejiang Province county.Monochamus alternatus aggregation pheromone 2-hendecane Oxy-1-ethanol is mixed with to n-hexane or the ethanolic solution that concentration is 0.5mg-100mg/100 microlitre, according to dosage, joins and in rubber stopper, make aggregation pheromone and lure core.Aggregation pheromone lures core to be suspended among trapper by thin wire.Trapper hangs on the generating region of pine nematode and Monochamus alternatus Hope, and in emphasis epidemic situation monitored space, each trapper is at a distance of 40-50 meters, and trapper is arranged on mountain top, circulation place of forest-road both sides air.Following 8 formulas (in Table 1) are set.Another 2 contrasts (the Zhejiang product M-99 of gloomy inspection office and applicant be the patent formulation of application in the past).In plastic bottle, add attractant dosage to be 80ml.Three repetitions are established in every kind of processing.
The different proportionings of table 2. at woodland to the attractant effect of Monochamus alternatus Hope (At Meishan, changxing, zhejiang Province county, 2013,6.10-30, N=3)
Figure BSA0000096340870000061
The result of table 2 and table 3 shows, the Monochamus alternatus Hope quantity that formula 1-8 lures at woodland will compare than 1 (patent formulation of applying for applicant before) and to contrast 2 (the product M-99 of Zhejiang gloomy inspection office) obviously many.The formula that applicant applied in the past adds after Monochamus alternatus Hope aggregation pheromone attractant 2-hendecane Oxy-1-ethanol again, and the allure of the female male imago of Monochamus alternatus Hope is brought up to 1.6-8.5 times.In addition,, after adding nopinene, than the formula that does not add nopinene, it has also improved 1.2-1.4 times of left and right to the allure of the female male imago of Monochamus alternatus Hope
Compare with the product M-99 of Zhejiang gloomy inspection office, this patent formula is brought up to 1.8-7.9 times to the allure of the female male imago of Monochamus alternatus Hope.
The different proportionings of table 3. at woodland to the attractant effect of Monochamus alternatus Hope (At Meishan, changxing, zhejiang Province county, 2013,7.01-10, N=3)
Figure BSA0000096340870000071
From above-mentioned each test, can find out, the attractant of producing with the product M-99 of current Zhejiang gloomy inspection office and the applicant in the past patent formulation of application compare, and the Monochamus alternatus Adults attractant of this patent formula, lures and lure effect even more ideal.And the advantages such as this patent formula has, and production technology is simple, 3-4 of formula constituents, with low cost, can effectively control Monochamus alternatus Hope woodland insect density, and naturally spreading of containment pine nematode is climing.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited to this, and any variation of expecting without creative work or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain that claims were limited.

Claims (6)

1. a Monochamus alternatus Hope attractant, it is characterized in that described Monochamus alternatus Hope attractant comprises: botanical attractant and aggregation pheromone 2-hendecane Oxy-1-ethanol, wherein botanical attractant comprises the component of percent by volume australene 60%~85%, nopinene 0~20%, ethanol 15%~40%, and the dosage of aggregation pheromone 2-hendecane Oxy-1-ethanol is that 0.5-100mg/ lures core.
2. attractant according to claim 1, the w/v of described nopinene is 1~20%, preferably 2~6%.
3. attractant according to claim 1 and 2, described brown longicorn beetle attractant does not comprise other active component.
4. hit a board-like funnel type trapper, wherein the botanical attractant described in claim 1-3 is arranged in the core that lures that hits board-like funnel type trapper, and aggregation pheromone claimed in claim 1 is arranged in rubber stopper.
5. above-mentioned attractant or the application of hitting board-like funnel type trapper, described is applied as for traping Monochamus alternatus Hope bisexuality adult and/or control pine nematode.
6. application according to claim 5, described trapper hangs on the generating region of pine nematode and Monochamus alternatus Hope.
CN201310488407.9A 2013-10-18 2013-10-18 Monochamus alternatus hope attractant and application thereof Active CN103563896B (en)

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Cited By (14)

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CN104542528A (en) * 2015-02-03 2015-04-29 东北林业大学 Anoplophora glabripennis visual effect improved intercepting baffle-type trap
CN105379711A (en) * 2015-12-01 2016-03-09 华南农业大学 Monochamus alternatus trapping and killing viscose glue
CN105530811A (en) * 2014-08-19 2016-04-27 朴永植 Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius
CN105875629A (en) * 2016-05-12 2016-08-24 华南农业大学 Luring and killing agent for monochamus alternatus
CN106538607A (en) * 2016-10-17 2017-03-29 浙江农林大学 A kind of Monochamus alternatus attractant and its using method
CN107258773A (en) * 2017-04-09 2017-10-20 华南农业大学 A kind of screening technique of Monochamus alternatus infochemical slow-release material
KR101805981B1 (en) * 2015-04-01 2017-12-07 대구대학교 산학협력단 A novel composition for controlling pine wilt disease, comprising 2-(Dodecyloxy)ethanol as an aggregation pheromone separated and identified from Monochamus alternatus in South Korea, and a method for preparation thereof
KR101811418B1 (en) * 2016-05-16 2017-12-22 이동하 Composition of pheromon and method for the preparation of sustained-release lure for attracting Monochamus saltuarius
CN108576025A (en) * 2018-06-01 2018-09-28 长乐智高生物科技有限公司 A kind of Monochamus alternatus attractant
CN108967425A (en) * 2018-08-31 2018-12-11 广西瑶瑶生物科技有限公司 Monochamus alternatus lures stick and its preparation method and application
CN109645003A (en) * 2019-01-19 2019-04-19 杭州科森农化有限公司 A kind of Monochamus alternatus Hope pheromone attractant
CN110771606A (en) * 2019-11-28 2020-02-11 南京林业大学 Adult longicorn luvifolius attractant and preparation method thereof
CN110896949A (en) * 2018-09-18 2020-03-24 苏州泽农生物科技有限公司 Water-based solid monochamus alternatus hope lure and preparation method thereof
CN111011375A (en) * 2019-11-11 2020-04-17 南京生兴有害生物防治技术股份有限公司 Novel preparation method of compound monochamus alternatus attractant and lure

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CN105530811B (en) * 2014-08-19 2018-01-02 Ad公司 Method for preventing and treating pine wood nematode disease by pre-observing and largely capturing monochamus alternatus and monochamus spruce flower monochamus alternatus
CN105530811A (en) * 2014-08-19 2016-04-27 朴永植 Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius
CN104542528A (en) * 2015-02-03 2015-04-29 东北林业大学 Anoplophora glabripennis visual effect improved intercepting baffle-type trap
CN104542528B (en) * 2015-02-03 2018-04-13 东北林业大学 Anoplophora glabripennis visual effect modified intercepts baffle-type trapper
KR101805981B1 (en) * 2015-04-01 2017-12-07 대구대학교 산학협력단 A novel composition for controlling pine wilt disease, comprising 2-(Dodecyloxy)ethanol as an aggregation pheromone separated and identified from Monochamus alternatus in South Korea, and a method for preparation thereof
CN105379711A (en) * 2015-12-01 2016-03-09 华南农业大学 Monochamus alternatus trapping and killing viscose glue
CN105875629A (en) * 2016-05-12 2016-08-24 华南农业大学 Luring and killing agent for monochamus alternatus
KR101811418B1 (en) * 2016-05-16 2017-12-22 이동하 Composition of pheromon and method for the preparation of sustained-release lure for attracting Monochamus saltuarius
CN106538607B (en) * 2016-10-17 2019-04-23 浙江农林大学 A kind of Monochamus alternatus attractant and its application method
CN106538607A (en) * 2016-10-17 2017-03-29 浙江农林大学 A kind of Monochamus alternatus attractant and its using method
CN109997878B (en) * 2016-10-17 2021-04-06 浙江农林大学 Monochamus alternatus trapping agent and using method thereof
CN109997878A (en) * 2016-10-17 2019-07-12 浙江农林大学 A kind of Monochamus alternatus trapping bait and its application method
CN107258773A (en) * 2017-04-09 2017-10-20 华南农业大学 A kind of screening technique of Monochamus alternatus infochemical slow-release material
CN108576025A (en) * 2018-06-01 2018-09-28 长乐智高生物科技有限公司 A kind of Monochamus alternatus attractant
CN108967425B (en) * 2018-08-31 2020-12-01 广西瑶瑶生物科技有限公司 Monochamus alternatus lure rod and preparation method and application thereof
CN108967425A (en) * 2018-08-31 2018-12-11 广西瑶瑶生物科技有限公司 Monochamus alternatus lures stick and its preparation method and application
CN110896949A (en) * 2018-09-18 2020-03-24 苏州泽农生物科技有限公司 Water-based solid monochamus alternatus hope lure and preparation method thereof
CN109645003A (en) * 2019-01-19 2019-04-19 杭州科森农化有限公司 A kind of Monochamus alternatus Hope pheromone attractant
CN111011375A (en) * 2019-11-11 2020-04-17 南京生兴有害生物防治技术股份有限公司 Novel preparation method of compound monochamus alternatus attractant and lure
CN110771606A (en) * 2019-11-28 2020-02-11 南京林业大学 Adult longicorn luvifolius attractant and preparation method thereof
CN110771606B (en) * 2019-11-28 2022-03-22 南京林业大学 Adult longicorn luvifolius attractant and preparation method thereof

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