CN102986662B - Adult monochamus alternatus attractant and using method thereof - Google Patents
Adult monochamus alternatus attractant and using method thereof Download PDFInfo
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- CN102986662B CN102986662B CN201210557269.0A CN201210557269A CN102986662B CN 102986662 B CN102986662 B CN 102986662B CN 201210557269 A CN201210557269 A CN 201210557269A CN 102986662 B CN102986662 B CN 102986662B
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Abstract
The invention discloses an adult monochamus alternatus attractant which comprises the components by volume content: 60-85% of alpha-pinene with the volume concentration of 95%, 15-30 parts of absolute ethyl alcohol and 0-20% of acetaldehyde with the volume concentration of 40%. The invention also discloses a using method of the adult monochamus alternatus attractant, which comprises the steps of: adding 80-200ml of the adult monochamus alternatus attractant into a plastic sustained-release tank and matching the plastic sustained-release tank with a trapper for use. The adult monochamus alternatus attractant can be used for monitoring the population quantity of adult monochamus alternatus, thus effectively reducing the density of the adult monochamus alternatus, and controlling the natural spread of pine wilt disease.
Description
Technical field
The present invention relates to a kind of Monochamus alternatus Hope (Monochamus alternatus Hope) imago attractant of propagating pine wood nematode, belong to field of pest control.
Background technology
Pine wood nematode (Bursaphelenchus xylophilus) is to cause pine tree destructive disease---the pathogen of pine nematode.China finds that there is pine nematode in Nanjing first in nineteen eighty-two, and in short twenties years, this disease is expanded and spread rapidly in China, has expanded at present national 110 Duo Ge counties and cities, causes the heavy damage of pine forest resource, and economic loss is huge.The generation of pine nematode and diffusion and host plant, vector insect are closely related.The most effective approach of controlling at present pine nematode is to control the occurrence and harm of vector insect, to reach the object of controlling pine nematode.The insect that occurring in nature can carry pine wood nematode has multiple, but as 13 kinds of the reports of vector insect, be all black sky Bos kind.The crucial vector insect of China is Monochamus alternatus Hope (Monochamus alternatus Hope).Monochamus alternatus Hope is also in forestry, to endanger serious tree borer.Generation also can cause pine wilt disease lethal separately.Exploitation inducing substance, utilizes attractant trapping Monochamus alternatus Hope and the pathogenic nematode that carries is a kind of effectively preventing measure.It can round-the-clock monitoring and controls Monochamus alternatus Hope, realizes the control to pine nematode.
The Main Ingredients and Appearance of the attractant (application number 200810028389) of the developments such as Chen Jiwen is pure natural extract from pine needles or host tree phloem ethanol extract and ethanol.This attractant has crucial active principle and the mechanism of action is unclear, processing technology is complicated, production cost is too high, simultaneously will be by the needle of host plant or bark lixiviate, and cut down and consume a large amount of pine trees.
The moth rod type destructive insect attractant (patent No. ZL03115289) of the developments such as Zhao Jinnian is mainly formed by australene, nopinene, acetaldehyde, acetone, alcohol, and wherein the percentage by weight of various compositions is: australene 30~70%, nopinene 2~25%, acetaldehyde 2~20%, acetone 8~38%, alcohol 10~40%.
The component of the Monochamus alternatus Adults attractant (application number 200910094345.7) of the developments such as Xiao Chun with and percent by volume be: turpentine oil 60~80%, ethanol 16~24%, benzyl carbinol 2~10%, terpineol 2~6%.The percent by volume of the each composition of Monochamus alternatus Adults attractant is: australene 24.64~32.86 %, nopinene 22.91~30.55%, 3-carene 6.51~8.68%, β-phellandrene 2.97~3.96%, terpinolene 0.74~0.99%, laurene 0.62~0.83%, other 1.60~2.13%, ethanol 16~24%, benzyl carbinol 2~10%, terpineol 2~6%.
All there is the shortcomings such as composition is many, processing technology is complicated, production cost is high in these attractants.
Summary of the invention
The technical problem to be solved in the present invention be to provide a kind ofly have that production technology is simple, with low cost, the Monochamus alternatus Adults attractant of the advantage such as safety, environmental protection, allure are strong, adopt this Monochamus alternatus Adults attractant can monitor the population quantity of Monochamus alternatus Adults, effectively reduce insect density, the diffusion naturally of containment pine nematode is climing.
In order to solve the problems of the technologies described above, the invention provides a kind of Monochamus alternatus Adults attractant, be grouped into by the one-tenth of following volume content:
Volumetric concentration is 95% australene 60%~85%;
Absolute ethyl alcohol 15%~30%;
Volumetric concentration is 40% acetaldehyde 0~20%.
Improvement as Monochamus alternatus Adults attractant of the present invention: the australene and the absolute ethyl alcohol that are 95% by volumetric concentration form, the australene that volumetric concentration is 95% and the volume ratio of absolute ethyl alcohol are 4:1 or are 5:1.
Another kind as Monochamus alternatus Adults attractant of the present invention improves: the acetaldehyde that the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by volumetric concentration are 40% forms, and the volume ratio of the acetaldehyde that australene, absolute ethyl alcohol and the volumetric concentration that described volumetric concentration is 95% is 40% is 3:1:1.
The present invention also provides the using method of above-mentioned Monochamus alternatus Adults attractant simultaneously: Monochamus alternatus Adults attractant 80~200ml is joined to plastics slowly-releasing bottle, coordinate trapper to use together.
Improvement as the using method of Monochamus alternatus Adults attractant of the present invention: plastics slowly-releasing bottle is that capacity is the plastics slowly-releasing bottle of 300ml.
Monochamus alternatus Adults attractant preparation method of the present invention is easy, and composition is simply uniformly mixed.
When the actual use of Monochamus alternatus Adults attractant of the present invention, this attractant 80~200ml is for example joined, in plastics slowly-releasing bottle (can produce 300ml plastics slowly-releasing bottle purchased from Taiyuan Xiaodian Area San Cheng plastic molding and processing plant), be used in combination together with trapper.Trapper for example can be selected the board-like funnel type trapper that hits of Xuanzhou, Anhui Province production.
The present invention forms through the repetition test of woodland.Compared with other existing attractants, there is the advantages such as easy to operate, with low cost, satisfactory for result.
Useful result of the present invention is: 1, this attractant all has remarkable attractant effect to Monochamus alternatus Hope bisexuality adult, can be used for the generation in Monochamus alternatus Hope field and the prediction of causing harm, and also can be directly used in control, reduces field population.2, use cost is cheap, and production technology is simple.
Embodiment
Now, in conjunction with the embodiment of the present invention, the invention will be further described, and further illustrate beneficial effect of the present invention by corresponding test.
Embodiment 1, a kind of Monochamus alternatus Adults attractant, the acetaldehyde that the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by volumetric concentration are 40% forms, and the volume ratio of the acetaldehyde that australene, absolute ethyl alcohol and the volumetric concentration that volumetric concentration is 95% is 40% is 3:1:1.
Embodiment 2, a kind of Monochamus alternatus Adults attractant, the australene and the absolute ethyl alcohol that are 95% by volumetric concentration form, and the australene that volumetric concentration is 95% and the volume ratio of absolute ethyl alcohol are 4:1.
Embodiment 3, a kind of Monochamus alternatus Adults attractant, the australene and the absolute ethyl alcohol that are 95% by volumetric concentration form, and the australene that volumetric concentration is 95% and the volume ratio of absolute ethyl alcohol are 5:1.
The volume ratio of the acetaldehyde that comparative example 1-1, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 4:1:1.
The volume ratio of the acetaldehyde that comparative example 1-2, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 6:1:1.
The volume ratio of the acetaldehyde that comparative example 1-3, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 8:1:1.
The volume ratio of the acetaldehyde that comparative example 1-4, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 2:1:1.
The volume ratio of the acetaldehyde that comparative example 1-5, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 1:1:1.
The volume ratio of the acetaldehyde that comparative example 1-6, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 1:2:1.
The volume ratio of the acetaldehyde that comparative example 1-7, the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by the volumetric concentration in embodiment 1 are 40% makes into: 1:1:3.
Comparative example 1-8, a kind of Monochamus alternatus Adults attractant, the acetaldehyde that nopinene, absolute ethyl alcohol and the volumetric concentration that the australene that is 95% by volumetric concentration, volumetric concentration are 95% is 40% forms, and above-mentioned 4 volume ratio is followed successively by 1:4:1.
Comparative example 2-1: the australene that is 95% by the volumetric concentration in embodiment 2 and the volume ratio of absolute ethyl alcohol make 1:1 into.
Comparative example 2-2: the australene that is 95% by the volumetric concentration in embodiment 2 and the volume ratio of absolute ethyl alcohol make 2:1 into.
Comparative example 2-3: the australene that is 95% by the volumetric concentration in embodiment 2 and the volume ratio of absolute ethyl alcohol make 3:1 into.
Comparative example 2-4: the australene that is 95% by the volumetric concentration in embodiment 2 and the volume ratio of absolute ethyl alcohol make 6:1 into.
Comparative example 2-5: the australene that is 95% by the volumetric concentration in embodiment 2 and the volume ratio of absolute ethyl alcohol make 1:3 into.
Comparative example 2-6, a kind of Monochamus alternatus Adults attractant, nopinene and absolute ethyl alcohol that the australene that is 95% by volumetric concentration, volumetric concentration are 95% form, and above-mentioned 3 volume ratio is followed successively by 1:1:1.
Comparative example 2-7, a kind of Monochamus alternatus Adults attractant, nopinene and absolute ethyl alcohol that the australene that is 95% by volumetric concentration, volumetric concentration are 95% form, and above-mentioned 3 volume ratio is followed successively by 3:1:1.
Comparative example 3, a kind of Monochamus alternatus Adults attractant, the acetaldehyde that the australene that is 95% by volumetric concentration and volumetric concentration are 40% forms, and the volume ratio of the australene that volumetric concentration is 95%, the acetaldehyde that volumetric concentration is 40% is 3:1.
The Monochamus alternatus Adults attractant that comparative example 4, Zhejiang gloomy inspection office buy, is mainly grouped into by following 5 kinds of one-tenth: australene, nopinene, acetaldehyde, acetone, alcohol.
Test 1:
What trapper selected that Xuanzhou, Anhui Province produces hit board-like funnel type trapper (made by horse spoken parts in an opera iron sheet. the umbrella hood that trapper top is footpath, end 41cm, cover is lower is long 50cm, 4 blocks of gear worm plates of wide 13cm, the middle top of plate installs one for laying the cylindrical core retainer plate (diameter 10cm, high 16cm) that lures that lures core, and jacket wall is covered with the circular hole of diameter 0.4cm; Gear worm plate below connects the funnel of upper bore 41cm, lower bore 9cm, funnel neck length 12cm, hopper walls becomes 45 DEG C of angles with vertical plane. when trapping, the filler neck bore of trapping is the plastic cup of 9cm, be mainly used in collecting the insect that trapping is arrived, evenly punch in its bottom surface and wall, keep polypide existing state to prevent that rainwater from soaking the insect that trapping is arrived).Lure core to be the 300ml plastics slowly-releasing bottle of tool slow release effect.In plastics slowly-releasing bottle, add attractant, test site is Songxi, Zhejiang pine forest.Trapper hangs on the generating region of pine nematode and Monochamus alternatus Hope, and in emphasis epidemic situation monitored space, each trapper is at a distance of 40-50 rice, and trapper is arranged on mountain top, circulation place of forest-road both sides air.In plastic bottle, add attractant dosage to be 120ml.Test site Zhejiang Chang Dong.Every processing repeats 4 times.
Above-described embodiment 1 ~ embodiment 3, comparative example 1-1 ~ comparative example 1-8, comparative example 2-1 ~ comparative example 2 ~ 7, comparative example 3, comparative example 4 are set as processing; Concrete outcome is as shown in table 1.
Table 1, woodland attractant effect (Zhejiang Chang Dong, 2011.7.12-8.16, N=4)
Test 2, make testing location into Zhejiang Songxi by Zhejiang Chang Dong, every processing repeats 5 times.All the other are with test 1.Result is as shown in table 2.
Table 2, woodland attractant effect (Songxi, Zhejiang, 2011,6.19-7.8, N=5)
Test 3, the Monochamus alternatus Adults attractant described in embodiment 3 is changed into Songxi, Zhejiang detect, dosage is respectively 50ml, 80ml, 120ml, 160ml, 200ml, 300ml.Every processing repeats 3 times.All the other are with test 1.Result is as shown in table 3.
Table 3, australene and ethanol is the attractant effect (Zhejiang Songxi, 2011.7.9-8.14, N=3) of various dose to Monochamus alternatus Hope in the time of 5/1 proportioning
As known from Table 3, lure core dosage in the time of 80~200ml, the Monochamus alternatus Hope quantity of luring is more.In the time that dosage is increased to 300ml, attractant effect obviously declines.
From above-mentioned each test, can find out, the Monochamus alternatus Adults attractant of the present invention's formula, attractant effect ideal, the attractant of producing with current Zhejiang is suitable.But the advantages such as formula of the present invention has production technology simple (formula constituent is only 2-3), with low cost, can effectively control Monochamus alternatus Hope woodland insect density, and the diffusion naturally of containment pine nematode is climing.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (3)
1. Monochamus alternatus Adults attractant, is characterized in that: the australene and the absolute ethyl alcohol that are 95% by volumetric concentration form, and the australene that described volumetric concentration is 95% and the volume ratio of absolute ethyl alcohol are 4:1 or are 5:1.
2. Monochamus alternatus Adults attractant, it is characterized in that: the acetaldehyde that the australene, absolute ethyl alcohol and the volumetric concentration that are 95% by volumetric concentration are 40% forms, the volume ratio of the acetaldehyde that australene, absolute ethyl alcohol and the volumetric concentration that described volumetric concentration is 95% is 40% is 3:1:1.
3. the using method of Monochamus alternatus Adults attractant as claimed in claim 1 or 2, is characterized in that: Monochamus alternatus Adults attractant 80~200ml is joined to plastics slowly-releasing bottle, coordinate trapper to use together; Described plastics slowly-releasing bottle is that capacity is the plastics slowly-releasing bottle of 300ml.
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CN103563896B (en) * | 2013-10-18 | 2015-04-22 | 浙江农林大学 | Monochamus alternatus hope attractant and application thereof |
CN105815350A (en) * | 2016-04-13 | 2016-08-03 | 南通清安健无水印染研究所(普通合伙) | Method for preparing microcapsule pesticide made by compounding insect attractant and insecticide and used for controlling monochamus alternatus |
CN107396895A (en) * | 2017-08-22 | 2017-11-28 | 华南农业大学 | A kind of Monochamus alternatus attractant long-acting slow-release control device |
CN111713495B (en) * | 2020-05-27 | 2022-03-22 | 漳州市英格尔农业科技有限公司 | Monochamus alternatus attractant, attraction device thereof, preparation method of attraction device and application of attraction device |
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CN1489903A (en) * | 2003-01-28 | 2004-04-21 | 浙江省森林病虫防治检疫站 | Attractants for insects to live in stems |
CN102027901A (en) * | 2010-12-07 | 2011-04-27 | 浙江农林大学 | Adult monochamus alternatus hope attractant |
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WO1987001561A1 (en) * | 1985-09-13 | 1987-03-26 | Borregaard Industries Limited | Composition for attraction of pine shoot beetles |
CN1489903A (en) * | 2003-01-28 | 2004-04-21 | 浙江省森林病虫防治检疫站 | Attractants for insects to live in stems |
CN102027901A (en) * | 2010-12-07 | 2011-04-27 | 浙江农林大学 | Adult monochamus alternatus hope attractant |
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Daniel R.Miller.Ethanol and (-)-α-Pinene: Attractant Kairomones for Some Large Wood-Boring Beetles in Southeastern USA.《J Chem Ecol》.2006,第32卷第779-794页. |
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