CN102499240A - Monochamus alternatus adult attractant based on insect patient-oriented pheromone, preparation method and applications - Google Patents
Monochamus alternatus adult attractant based on insect patient-oriented pheromone, preparation method and applications Download PDFInfo
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Abstract
The invention discloses an Monochamus alternatus adult attractant based on insect patient-oriented pheromone and applications thereof, belonging to the technical field of pest control. The Monochamus laternatus adult attractant based on insect patient-oriented pheromone is characterized in that a key component is 2-undecyloxy-1-ethanol; auxiliary components consist of alpha-pinene of purity 95% and acetaldehyde of purity 40%; and the volume ratio of the alpha-pinene of purity 95% to the acetaldehyde of purity 40% is 2-4:1. According to the monochamus alternatus adult attractant based on insect patient-oriented pheromone and the applications, the insect patient-oriented pheromone, namely 2-undecyloxy-1-ethanol, is screened successfully, the attractant is used together with a traditional botanical attractant, the inducing effect is improved by more than 5 times, especially in the later period (spawning period) of an adult, the trapping effect of the attractant is dozens of times of that of the botanical attractant. The attractant of the invention has the advantages of low cost, long effective duration, good safety, environmental friendliness and the like, can control the harm of Monochamus alternatus, has important significance on restraining the propagation and spreading of pine wood nematode disease, and fills the blank of home and abroad technology.
Description
Technical field
The invention belongs to the control of insect technical field, be specially a kind of Monochamus alternatus imago attractant and preparation method and purposes based on insect source sex pheromone.
Background technology
Pine nematode is called as pine tree " cancer ", and its cause of disease pine wood nematode is the first Alien invasive species of China's forestry, and Monochamus alternatus is its main communication media in China.At present, adopting plant resource attractant trapping Monochamus alternatus imago is the most frequently used one of the method for preventing and treating, but plant resource attractant trapping effect is not very good, and this also becomes the more main cause of wide popularization and application of restriction Monochamus alternatus attractant.On agricultural, the development of moth class insect sex pheromone and application are very extensive, like cotton bollworm, fall webworm etc.But the development of coleoptera beetle insect sex pheromone and application are fewer comparatively speaking.Monochamus alternatus belongs to the coleoptera Cerambycidae, and its sex pheromone does not also successfully extract and identifies out.Most of in the world experts think that a day bovine insect is through plant resource information location host plant, and trafficability characteristic pheromones location spouse in short range thinks that promptly longicorn sex pheromone coverage is very short then.
At present; Attractant product in the practical application mainly is the crude extract matter that extracts from weak host plant, and crucial active principle, synergistic matter and the mechanism of action thereof are unclear, attractant effect is undesirable, processing technology is complicated, production cost is crossed problems such as height yet still exist.The attractant (application number 200810028389) of development such as Chen Jiwen, Main Ingredients and Appearance is pure natural extract from pine needles or host tree phloem ethanol extract and ethanol.There is following shortcoming in this attractant: 1) concrete effective ingredient is unclear, and the crude extract matter that its attractant composition is a host plant does not work out its particular compound composition; 2) producing process is complicated, need be with needle or the bark lixiviate of host plant, and cut down and consume a large amount of pine trees.The moth rod type destructive insect attractant of Zhao Jinnian etc. development (application number/patent No.: 03115289.9), i.e. M99-1 attractant, mainly formulated by australene, nopinene, acetaldehyde, solvent.There is following shortcoming in this attractant: 1) be the plant resource material: Main Ingredients and Appearance all derives from plant; 2) attractant effect is undesirable.The attractant (application number 200510028194.7) of yellow brave equality development, Main Ingredients and Appearance are 25-35% (v/v) turpentine oil (Turpentine) and 5-10% (v/v) australene (R-α-Pinene) and 5%-7% (v/v) nopinene (β-Pinene) and 5-8% (v/v) laurene (Myrcene) and 5-12% (v/v) carene ((3)-Carene) and 5-10 (v/v) alpha-terpineol (α-Terpineol) and the combination of 30-38% (v/v) absolute ethyl alcohol.There is following shortcoming in this attractant: 1) composition is complicated, production cost is high, and the concrete effective ingredient of its attractant is very complicated, reaches 7 kinds more than, causes production cost higher; 2) attractant effect is undesirable; 3) poor to getting food phase adult attractant effect, the inventor is through comparative test result demonstration for many years, and the food phase of the getting adult that this attractant is lured accounts for about 50% of whole trapping adult total amounts.Number of patent application is 201010577064.X Monochamus alternatus imago attractant, is made up of 95% australene and 40% acetaldehyde, and there is following shortcoming in this attractant: 1) be the plant resource material: Main Ingredients and Appearance all derives from plant; 2) attractant effect is undesirable.
Summary of the invention
To the problems referred to above that exist in the prior art situation, the objective of the invention is to design provides a kind of based on the Monochamus alternatus imago attractant of insect source sex pheromone and the technical scheme of purposes, and its attractant effect is greatly improved.
Described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that crucial composition is 2-hendecane Oxy-1-ethanol; Auxiliary composition is that 95% australene and 40% acetaldehyde are formed 95% australene by purity: the volume ratio of 40% acetaldehyde is 2-4:1.
Described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone, the rate of volatilization that it is characterized in that 2-hendecane Oxy-1-ethanol is 0.5-3mg/d, the rate of volatilization of 95% australene and 40% acetaldehyde mixture is 2000-6000 μ L/d.
Described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 20-200mg:100-800ml.
Described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that 95% australene: the volume ratio of 40% acetaldehyde is 2.5-3.5:1.
Described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that 95% australene: the volume ratio 3:1 of 40% acetaldehyde.
The preparation method of described a kind of Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that:
1) 2-hendecane Oxy-1-ethanol is added drop-wise on the filter fiber, then filter fiber is packed in the polyethylene slowly-releasing bag, process the insect source and lure core;
2) be that 95% australene and 40% acetaldehyde of the 2-4:1 polyethylene of packing into discharges bottle with volume ratio, rate of volatilization is 2000-6000 μ L/d, processes plant resource and lures core.
The application of 2-hendecane Oxy-1-ethanol in the preparation Monochamus alternatus imago attractant.
Above-mentioned 2-hendecane Oxy-1-ethanol can be buied by Spain organic synthesis Co., Ltd; Australene (95%) can be buied by desert, Guangzhou chemical industry Co., Ltd, and acetaldehyde (40%) can be buied by Guangdong Guanghua Science and Technology Co., Ltd..
Above-mentioned a kind of Monochamus alternatus imago attractant and purposes based on insect source sex pheromone; Successfully filter out a kind of insect source sex pheromone: 2-hendecane Oxy-1-ethanol; Be combined together use with traditional plant source attractant; Attractant effect is improved more than five times, and especially the later stage of adult is the egg-laying period, and trap effect is tens times of plant resource attractant; And advantages such as that this attractant has is with low cost, lasting period length, safety, environmental protection can effectively be controlled Monochamus alternatus harm, for the propagation of containment pine nematode with spread and be significant, fill up domestic and international technological gap.
The percentage composition that relates in the present specification unless otherwise indicated, other all refers to weight percentage.
Description of drawings
Fig. 1 is that different attractants are to Monochamus alternatus attractant effect comparison diagram;
Among the figure: F1: volume ratio is 95% australene of 3:1: the attractant that 40% acetaldehyde is formed; S:2-hendecane Oxy-1-ethanol; F1+S: the present invention fills a prescription 2; PIS is European pinus sylvestris var. mongolica China ink longicorn attractant; H is 2-hexanol+methyl-n-butyl ketone+3-hexanol+3-hexanone; M99-1 is the attractant of Zhejiang Province's forest prevention station and Subtropical Zone Forestry Inst., Chinese Academy of Forest Science's joint research and development, and A-3 is the attractant of forest-science academy, Guangdong Province development, and QS is the attractant that Chinese Academy of Sciences's Shanghai plant physiology is developed.
Embodiment
Combine embodiments of the invention at present, the present invention is described further, and further specify beneficial effect of the present invention through corresponding test.
Prescription 1:2-hendecane Oxy-1-ethanol; Volume ratio is 95% australene, 40% acetaldehyde of 2:1, and 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 20mg:100ml.
Prescription 2:2-hendecane Oxy-1-ethanol; Volume ratio is 95% australene, 40% acetaldehyde of 3:1, and 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 45mg:200ml.
Prescription 3:2-hendecane Oxy-1-ethanol; Volume ratio is 95% australene, 40% acetaldehyde of 4:1, and 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 100mg:400ml.
Prescription 4:2-hendecane Oxy-1-ethanol; Volume ratio is 95% australene, 40% acetaldehyde of 2.5:1, and 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 150mg:600ml.
Prescription 5:2-hendecane Oxy-1-ethanol; Volume ratio is 95% australene, 40% acetaldehyde of 3.5:1, and 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 200mg:800ml.
The inventor is through research for many years; Successfully filter out a kind of insect source sex pheromone, promptly 2-hendecane Oxy-1-ethanol is applied on the Monochamus alternatus attractant; Make traditional attractant effect that the raising more than five times arranged; In Monochamus alternatus sex pheromone research, be successful Application first, filled up domestic and international blank.This attractant has advantages such as low production cost, lasting period length, safety, environmental protection in addition, for the control Monochamus alternatus, the containment pine nematode spread and propagation is significant.
The step following steps of screening crucial composition are said: collect the insect volatile matter with atmosphere sampling instrument; Analyze the insect compound with the gaseous mass spectrum combined instrument; With tentaculum electric potential combined instrument screening key compound; Carry out indoor behavior activity test with Y type olfactometer, carry out open-air activity test with trapper, finishing screen is selected crucial composition.
The method for using of above-mentioned Monochamus alternatus imago attractant is following:
The insect source lures core and plant resource to lure the releasing device of core inequality; The insect source lures the core releasing device for containing the polyethylene slowly-releasing bag (2.5 cm * 2.5 cm * 120 μ m are thick) of filter fiber (15 * 6 mm); Contain 45 mg 2-hendecane Oxy-1-ethanol in the filter fiber, rate of volatilization is 1mg/d; It is that polyethylene discharges bottle that plant resource lures the core releasing device, and containing the 200ml volume ratio is 95% australene and 40% acetaldehyde (promptly filling a prescription 2) of 3:1, and rate of volatilization is 4000 μ L/d.Attractant was changed once in per 45 days.Adopt the cross type trapper; On adjacent two trees, bind a waddy, on waddy, hang trapper, the trapper height is about 80cm; Hang up polyethylene slowly-releasing bag and polyethylene that A and B component are housed on the trapper and discharge bottle, be separated by between the adjacent trapper 20~30 meters.Result, the quantity of detail record longicorn, sex, fish brood amount etc. are once traped in inspection in per 6 days.Described polyethylene slowly-releasing bag and polyethylene discharge all can be from the Shanxi grand will of bottle Plastic Products Co., Ltd and buy.Show from the trapping result of table 1: 2 attractant effects of filling a prescription are best, are superior to filling a prescription 1, prescription 3~5.In addition, 1 carry out identical trapping test, also can obtain beneficial effect of the present invention, use attractant effect good separately, remarkable synergistic effect is arranged than firpene or sex pheromone with prescription 3~5 to fill a prescription.
The different embodiment of table 1 are to the open-air trap effect contrast table of Monochamus alternatus imago
Prescription | Total trapping amount (head) | Average trapping amount (head/inferior) |
Prescription 1 | 645 | 46.07 |
Prescription 2 | 846 | 60.43 |
|
725 | 51.79 |
Prescription 4 | 699 | 49.92 |
Prescription 5 | 730 | 52.14 |
The l trapping time be on May 10th, 2011 to August 7, investigated once result in per 6 days.
The rate of volatilization that the insect source lures core also can be 0.5,1.0,2.0 or 3.0mg/d; Plant resource lures the rate of volatilization of core also can be 2000,3000,4000,5000 or 6000 μ L/d; Two kinds that place in polyethylene slowly-releasing bag and the polyethylene release bottle lure the consumption of core to be provided with according to the trapping fate, generally with attractant of replacing in 30-60 days.
Below further specify beneficial effect of the present invention according to comparative trial again.
Test 1: the present invention fill a prescription F1+S (prescription 2) and the prescription in inventor oneself former years and now on the market widely used attractant product compare test.Wherein, F1 is that volume ratio is 95% australene, 40% acetaldehyde of 3:1; S is 2-hendecane Oxy-1-ethanol; H is 2-hexanol, methyl-n-butyl ketone, 3-hexanol and 3-hexanone, is Monochamus alternatus body surface isolation identification compound, is bought by Shanghai lark prestige reagent company; PIS is European pinus sylvestris var. mongolica China ink longicorn attractant, and composition is bark beetle enol+2-methyl-3-butene-2-alcohol+australene+2-hendecane Oxy-1-ethanol, can be buied by company of Spain organic synthesis Co., Ltd; M99-1 is Zhejiang Province's forest prevention station and Subtropical Zone Forestry Inst., Chinese Academy of Forest Science's joint research and development, and A-3 is the development of forest-science academy, Guangdong Province, and QS is developed by Chinese Academy of Sciences's Shanghai plant physiology, and comparative test result is as shown in Figure 1.
Result of the test shows: the present invention fill a prescription F1+S (prescription 2) synergistic effect very significantly; Trap effect improves 4.5 times than former attractant " volume ratio is 95% australene of 3:1: 40% acetaldehyde agent " prescription; Especially be the stage in egg-laying period in the later stage of Monochamus alternatus imago, trap effect improves tens times than original formulation F1.But sex pheromone uses separately, and effect and original formulation F1 are approximate.Through comparative analysis; The present invention's F1+S effect of filling a prescription significantly is superior to other attractant product; Wherein improve 34%, improve 10 times, produce the M99-1 effect than Zhejiang and improve nearly 6 times than the effect of F1+H than European pinus sylvestris var. mongolica China ink longicorn attractant PIS effect; Improve nearly 7 times than last marine products QS effect, produce the A-3 effect than Fujian and improve more than 8 times.With fill a prescription 1 with the prescription 3-5 carry out attractant product comparative trial, also can obtain beneficial effect of the present invention.
Inferring with attractant composition synergy principle, is that 2-hendecane Oxy-1-ethanol is united use with prescription M99-1, A-3 or QS with prescription S, also can obtain the similar synergistic effect of the present invention.Rate of volatilization when 2-hendecane Oxy-1-ethanol is used with prescription M99-1, A-3 or QS and of the present invention approaching.Explain that 2-hendecane Oxy-1-ethanol uses in the Monochamus alternatus imago attractant in preparation, can significantly improve its attractant effect.
Claims (7)
1. the Monochamus alternatus imago attractant based on insect source sex pheromone is characterized in that crucial composition is 2-hendecane Oxy-1-ethanol; Auxiliary composition is that 95% australene and 40% acetaldehyde are formed 95% australene by purity: the volume ratio of 40% acetaldehyde is 2-4:1.
2. a kind of Monochamus alternatus imago attractant as claimed in claim 1 based on insect source sex pheromone; The rate of volatilization that it is characterized in that 2-hendecane Oxy-1-ethanol is 0.5-3mg/d, and the rate of volatilization of 95% australene and 40% acetaldehyde mixture is 2000-6000 μ L/d.
3. a kind of Monochamus alternatus imago attractant based on insect source sex pheromone as claimed in claim 1 is characterized in that 2-hendecane Oxy-1-ethanol: the w/v of 95% australene and 40% acetaldehyde mixture is 20-200mg:100-800ml.
4. a kind of Monochamus alternatus imago attractant based on insect source sex pheromone as claimed in claim 1 is characterized in that 95% australene: the volume ratio of 40% acetaldehyde is 2.5-3.5:1.
5. a kind of Monochamus alternatus imago attractant based on insect source sex pheromone as claimed in claim 1 is characterized in that 95% australene: the volume ratio 3:1 of 40% acetaldehyde.
6. the preparation method of a kind of Monochamus alternatus imago attractant based on insect source sex pheromone as claimed in claim 1 is characterized in that:
1) 2-hendecane Oxy-1-ethanol is added drop-wise on the filter fiber, then filter fiber is packed in the polyethylene slowly-releasing bag, process the insect source and lure core;
2) be that 95% australene and 40% acetaldehyde of the 2-4:1 polyethylene of packing into discharges bottle with volume ratio, rate of volatilization is 2000-6000 μ L/d, processes plant resource and lures core.
7.2-the application of hendecane Oxy-1-ethanol in the preparation Monochamus alternatus imago attractant.
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CN103210949A (en) * | 2013-03-29 | 2013-07-24 | 浙江农林大学 | Adult anoplophora glabripennis attractant |
KR101530827B1 (en) * | 2014-08-19 | 2015-06-24 | 박영식 | Control method of pine wood nematode by monitoring and mass-trapping of Monochamus aternatus and Monochamus saltuarius |
CN105052860A (en) * | 2015-07-23 | 2015-11-18 | 福建农林大学 | Attracting core of monochamus alternatus hope adult attractant and preparation method thereof |
CN109645003A (en) * | 2019-01-19 | 2019-04-19 | 杭州科森农化有限公司 | A kind of Monochamus alternatus Hope pheromone attractant |
CN109793013A (en) * | 2019-02-02 | 2019-05-24 | 杭州科森农化有限公司 | A kind of coleopteron attractant |
CN109997878A (en) * | 2016-10-17 | 2019-07-12 | 浙江农林大学 | A kind of Monochamus alternatus trapping bait and its application method |
CN110896949A (en) * | 2018-09-18 | 2020-03-24 | 苏州泽农生物科技有限公司 | Water-based solid monochamus alternatus hope lure and preparation method thereof |
CN111011375A (en) * | 2019-11-11 | 2020-04-17 | 南京生兴有害生物防治技术股份有限公司 | Novel preparation method of compound monochamus alternatus attractant and lure |
CN111713495A (en) * | 2020-05-27 | 2020-09-29 | 漳州市英格尔农业科技有限公司 | Monochamus alternatus attractant, attraction device thereof, preparation method of attraction device and application of attraction device |
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CN103210949A (en) * | 2013-03-29 | 2013-07-24 | 浙江农林大学 | Adult anoplophora glabripennis attractant |
CN105530811B (en) * | 2014-08-19 | 2018-01-02 | Ad公司 | Pass through the method for the monitoring and a large amount of capture preventing and treating pine nematodes of Monochamus alternatus and the black longicorn of dragon spruce |
KR101530827B1 (en) * | 2014-08-19 | 2015-06-24 | 박영식 | Control method of pine wood nematode by monitoring and mass-trapping of Monochamus aternatus and Monochamus saltuarius |
CN105530811A (en) * | 2014-08-19 | 2016-04-27 | 朴永植 | Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius |
CN105052860A (en) * | 2015-07-23 | 2015-11-18 | 福建农林大学 | Attracting core of monochamus alternatus hope adult attractant and preparation method thereof |
CN105052860B (en) * | 2015-07-23 | 2017-10-13 | 福建农林大学 | A kind of Monochamus alternatus Adults attractant lure and preparation method thereof |
CN109997878A (en) * | 2016-10-17 | 2019-07-12 | 浙江农林大学 | A kind of Monochamus alternatus trapping bait and its application method |
CN109997878B (en) * | 2016-10-17 | 2021-04-06 | 浙江农林大学 | Monochamus alternatus trapping agent and using method thereof |
CN110896949A (en) * | 2018-09-18 | 2020-03-24 | 苏州泽农生物科技有限公司 | Water-based solid monochamus alternatus hope lure and preparation method thereof |
CN109645003A (en) * | 2019-01-19 | 2019-04-19 | 杭州科森农化有限公司 | A kind of Monochamus alternatus Hope pheromone attractant |
CN109793013A (en) * | 2019-02-02 | 2019-05-24 | 杭州科森农化有限公司 | A kind of coleopteron attractant |
CN111011375A (en) * | 2019-11-11 | 2020-04-17 | 南京生兴有害生物防治技术股份有限公司 | Novel preparation method of compound monochamus alternatus attractant and lure |
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CN111713495B (en) * | 2020-05-27 | 2022-03-22 | 漳州市英格尔农业科技有限公司 | Monochamus alternatus attractant, attraction device thereof, preparation method of attraction device and application of attraction device |
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