CN111713495A

CN111713495A - Monochamus alternatus attractant, attraction device thereof, preparation method of attraction device and application of attraction device

Info

Publication number: CN111713495A
Application number: CN202010459425.4A
Authority: CN
Inventors: 关雄; 何学友; 蔡守平; 金化亮; 梁景华; 林永旋; 游世奇
Original assignee: FUJIAN ACADEMY OF FORESTRY; Fujian Inger Biotechnology Co ltd; ZHANGZHOU ENJOY AGRICULTURAL TECHNOLOGY CO LTD
Current assignee: FUJIAN ACADEMY OF FORESTRY; Fujian Inger Biotechnology Co ltd; ZHANGZHOU ENJOY AGRICULTURAL TECHNOLOGY CO LTD
Priority date: 2020-05-27
Filing date: 2020-05-27
Publication date: 2020-09-29
Anticipated expiration: 2040-05-27
Also published as: CN111713495B

Abstract

The invention relates to a monochamus alternatus attractant, an attraction device thereof, a preparation method and application of the attraction device, wherein the monochamus alternatus attractant comprises a plant source pheromone and an aggregation pheromone, and the plant source pheromone and the aggregation pheromone are mutually synergistic to realize attraction of monochamus alternatus; the plant source pheromone comprises the following components in percentage by volume: 50-80% of turpentine, 10-40% of ethanol and 5-10% of acetaldehyde; the aggregation pheromone is 2-undecyloxy-1-ethanol. The monochamus alternatus attractant has a good attraction effect. The inducing device comprises a first slow release body and a second slow release body, wherein the first slow release body and the second slow release body are simultaneously installed on a Monochamus alternatus trap to release aggregation pheromone and plant source pheromone. The luring device is convenient to use, good in slow release effect and long in drug effect period, and can greatly reduce the population density of monochamus alternatus hope and inhibit the spread and propagation of pine wood nematode disease.

Description

Monochamus alternatus attractant, attraction device thereof, preparation method of attraction device and application of attraction device

Technical Field

The invention relates to the technical field of insect prevention and control, in particular to a monochamus alternatus attractant, an attracting device thereof, a preparation method of the attracting device and application of the attracting device.

Background

Monochamus alternatus (Monlchamus alternatus Hope) belonging to the order Coleoptera, Anoplophorae, Anoplopodae subfamily, Monochamus. The Monochamus alternatus hope not only damages trees with weak growth vigor by boring the larvae, but also damages xylem and phloem of the trees, causes insufficient or interrupted transportation of water and nutrients, seriously influences the growth of the pine trees, and even causes the pine trees to die in pieces. More serious is that the adult monochamus alternatus is also the main transmission medium of the pine destructive disease-pine wood nematode disease (Bursaphelenchus xylophilus) which is worldwideResearchers have called "cancer of pine trees". The pine wilt disease was first discovered in Nakazaki city in 1905 in Japan, after which it spread. Since the first discovery of pine wood nematode disease on black pine of Shanling in Nanjing City in 1982, the wood loss of China exceeds 500 ten thousand meters³Resulting in a direct economic loss of about 25 billion dollars. The pine wood nematode disease destroys the natural landscape and forest ecological environment of China, poses serious threat to the sustainable and healthy development of the forestry of China, and brings great loss to national economy and people's life. The key to preventing and controlling the pine wood nematode disease is to cut off the transmission medium of the pine wood nematode disease. At present, methods such as tree cleaning for death of diseases, chemical pesticide spraying, attractant prevention and control and the like are mainly adopted for treating the pine wood nematode disease, wherein the attractant technology has the characteristics of greenness, environmental protection, high efficiency and the like, and is one of the most common methods for preventing and controlling adult monochamus alternatus.

Patent application CN102986739A discloses an adult monochamus alternatus attractant. The attractant comprises an artificially synthesized compound prepared by adding reagents such as undecanol, thionyl chloride, ethylene glycol, metallic sodium and the like. According to the scheme, after the plant source pheromone and the monochamus alternatus gathering pheromone are mixed, a technology of releasing by a plastic slow-release bag is adopted, an effective synergist is lacked in a formula, the trapping effect is influenced, in addition, because the gathering pheromone and turpentine have different requirements on the volatilization rate, the gathering pheromone and the turpentine are mixed together and are all released by the slow-release bag, small molecular substances volatilize firstly, large molecular substances volatilize later, the proportion of the formula at the later stage is easy to unbalance seriously, the trapping effect is reduced, and meanwhile, the lasting period is.

Disclosure of Invention

The invention aims to solve the problems of poor attracting and trapping effect and short duration of the existing monochamus alternatus, and provides the monochamus alternatus attractant which has good attracting effect and can be better applied to prevention and treatment of monochamus alternatus.

In order to achieve the purpose, the technical scheme of the invention is as follows: the Monochamus alternatus attractant comprises plant source pheromone and aggregation pheromone, wherein the plant source pheromone and the aggregation pheromone are mutually synergistic to attract Monochamus alternatus;

the plant source pheromone comprises the following components in percentage by volume: 50-80% of turpentine, 10-40% of ethanol and 5-10% of acetaldehyde; the aggregation pheromone is 2-undecyloxy-1-ethanol.

Preferably, the mass ratio of the plant source pheromone to the aggregation pheromone is (20-30): (1-2).

Preferably, the plant source pheromone consists of the following components in percentage by volume: 55-75% of turpentine, 15-35% of ethanol and 6-9% of acetaldehyde;

further, optionally, the volume ratio of turpentine to ethanol in the plant source pheromone is (1.5-4) to 1;

further, optionally, the volume ratio of turpentine, ethanol and acetaldehyde in the plant source pheromone is (5.5-6.5): (2.5-3.5): (0.5-1.5).

Further, the aggregation pheromone further comprises farnesene, and the farnesene and the 2-undecyloxy-1-ethanol are mixed with each other.

Preferably, the volume ratio of the 2-undecyloxy-1-ethanol to the farnesene in the aggregation pheromone is (6-70): 1; more preferably (50-65): 1.

The monochamus alternatus attractant comprises plant source pheromones and aggregation pheromones, and the monochamus alternatus is attracted under the synergistic effect of the two pheromones, so that the effects are superposed, and the attraction effect is good. Particularly, the plant source pheromone is formed by mixing turpentine, ethanol and acetaldehyde according to a proper proportion, the smell of natural pine trees is better simulated, wherein the acetaldehyde plays a role in synergism, and the volume ratio is controlled to be below 10 percent so as to prevent the smell from generating great change; and the aggregation pheromone generates a clustering effect, so that the attracting effect is effectively improved, and the monochamus alternatus hope is better attracted.

The invention also provides a trapping device containing the monochamus alternatus attractant, which has a good slow release effect and long drug effect duration, is matched with a monochamus alternatus trapper for use, can greatly reduce the population density of monochamus alternatus, and inhibits the spread and propagation of pine wilt disease.

The specific scheme is as follows: a Monochamus alternatus attractant device comprises a first slow release body and a second slow release body, wherein the first slow release body comprises a first slow release bag, a cotton core body is filled in the first slow release bag, a plurality of capsules are absorbed in the cotton core body, the aggregation pheromone is stored in the capsules, and the opening part of the first slow release bag is sealed; the second slow release body comprises a second slow release bag, cotton is filled in the second slow release bag, the cotton adsorbs the plant source pheromone, and the opening part of the second slow release bag is sealed; the first slow release body and the second slow release body are simultaneously arranged on a monochamus alternatus trap to release aggregation pheromone and plant source pheromone.

The cotton core body is arranged in a plastic pipe with at least one open end, and the plastic pipe is arranged in the first slow release bag; to further improve the sustained release effect.

The mounting distance between the first slow release body and the second slow release body on the Monochamus alternatus trap is less than or equal to 20 cm. Synergy between plant-derived pheromones and aggregated pheromones has been ensured.

Preferably, the first sustained-release bag is made of polyethylene, and the density of the polyethylene is in the range of 0.910-0.9708g/cm³The wall thickness of the first slow release bag is 150-;

preferably, the second sustained-release bag is made of polyethylene, and the density of the polyethylene is in the range of 0.910-0.9708g/cm³(ii) a The wall thickness of the second slow release bag is 20-200 mu m;

preferably, the plastic pipe is a polyethylene pipe, and the wall thickness of the plastic pipe is 200-400 mu m;

furthermore, the residual section after the first slow release bag opening is sealed and the residual section after the second slow release bag opening is sealed are detachably connected together. So as to be convenient for transportation and use.

Further, a hanging through hole is formed in the sealed remaining section of the first slow release bag opening and/or the sealed remaining section of the second slow release bag opening. So that the first slow release bag and the second slow release bag are mounted in a suspension manner.

The monochamus alternatus attractant device provided by the invention has a good slow-release effect and a long drug effect duration period, is convenient to be used in cooperation with a trap, can greatly reduce the population density of monochamus alternatus and inhibit the spread and propagation of pine wilt disease.

The invention further provides a preparation method of the monochamus alternatus luring device, which comprises the following steps:

step 1: mixing turpentine, ethanol and acetaldehyde according to the volume percentage to form plant source pheromone liquid, filling a set amount of cotton into a second slow release bag, adding a set amount of plant source pheromone liquid into the second slow release bag to enable the plant source pheromone liquid to be adsorbed in the cotton, and then sealing the opening of the second slow release bag to prepare a second slow release body;

step 2: encapsulating the aggregation pheromone in a capsule shell by a microcapsule embedding technology to prepare capsules, adsorbing a plurality of the capsules on a cotton core body, then arranging the cotton core body in a plastic tube with at least one open end, arranging the plastic tube in a first sustained-release bag, and sealing the opening part of the first sustained-release bag to prepare a first sustained-release body; the first and second delay bodies may be prepared simultaneously or in any order.

And step 3: and detachably connecting the sealed residual section of the first slow release bag opening part and the sealed residual section of the second slow release bag opening part together to form the trapping device.

The detachable connection is through the staple connection or through the binding together of connecting strip.

The preparation method provided by the invention has simple steps, is easy to realize and is convenient for forming factory operation.

Further, the invention also provides the application of the monochamus alternatus luring device, the luring device is hung or placed in the monochamus alternatus trap, and the luring device releases monochamus alternatus luring agent to trap monochamus alternatus into the monochamus alternatus trap to be caught. The population density of monochamus alternatus hope can be greatly reduced, and the spread of the pine wood nematode disease can be restrained. .

Drawings

In order to illustrate the technical solution of the present invention more clearly, the drawings will be briefly described below, and it is apparent that the drawings in the following description relate only to some embodiments of the present invention and are not intended to limit the present invention.

FIG. 1 is a schematic structural diagram of a first sustained release body according to an embodiment of the present invention;

FIG. 2 is an enlarged view at A of FIG. 1;

FIG. 3 is a schematic structural view of a second sustained release body according to an embodiment of the present invention;

FIG. 4 is a schematic view of the first and second pouches being connected together by staples in accordance with the present invention;

FIG. 5 is a schematic view showing that a first sustained-release pouch and a second sustained-release pouch of the present invention are connected together by a connection band.

FIG. 6 is a graph showing the trapping effect of the trapping test in which the plant-derived pheromone is unchanged and the aggregation pheromone is changed in example 1 of the present invention;

FIG. 7 is a graph showing the trapping effect of the trapping test in which the pheromone was aggregated without changing the plant-derived pheromone in example 2 of the present invention;

FIG. 8 is a graph comparing the trapping results of the A8 test group of Table 1 in comparative example 1 and example 1 of the present invention;

FIG. 9 is a graph showing the trapping results of three test groups in comparative example 2 of the present invention.

Detailed Description

The definitions of some of the terms used in the present invention are given below, and other non-mentioned terms have definitions and meanings known in the art:

plant source pheromone: phytopheromones are present in many plants as compounds of the terpene (ti) family. It has volatile odor and is mainly distributed in stems, leaves, roots and flowers of plants. The substance not only can promote the growth of plants, but also has delicate effect with the environment. In the invention, the plant source pheromone comprises the following components in percentage by volume: 50-80% of turpentine, 10-40% of ethanol and 5-10% of acetaldehyde. Preferably: 55-75% of turpentine, 15-35% of ethanol, 6-9% of acetaldehyde, more preferably: 60-70% of turpentine, 20-30% of ethanol and 6-9% of acetaldehyde, for example: 62-68% of turpentine, 22-28% of ethanol and 7-8% of acetaldehyde.

The components interact with each other to play a comprehensive role in mixed smell, preferably, the ethanol can be used as a synergist for the turpentine to lure monochamus alternatus imagoes, and can also obviously improve the female-luring capacity, so that the ethanol is also a very important substance in the monochamus alternatus host selection process, and the volume ratio of the turpentine to the ethanol is (1.5-4):1, preferably 2: 1. On the basis of mixing turpentine and ethanol, acetaldehyde can further enhance the trapping effect and exert the advantages of turpentine and ethanol, and the volume ratio of turpentine to ethanol to acetaldehyde is (5.5-6.5): (2.5-3.5): (0.5-1.5), preferably (5.5-6.5): (2.5-3.5): 1, more preferably 6: 3: 1. it should be noted that the volume fraction of acetaldehyde cannot exceed 10%, otherwise the odor of other components is masked and the trapping effect is poor.

Aggregation pheromones: aggregation pheromones are hormones secreted by a group of insects that find food sources, parasites or aggregate collective migratory flight tolerance in certain individuals. Other individuals smell the pheromone odor and gather it toward the target. In the present invention, the aggregation pheromone is 2-undecyloxy-1-ethanol, preferably a mixture of 2-undecyloxy-1-ethanol and farnesene, 2-undecyloxy-1-ethanol mainly functions as aggregation pheromone, farnesene is a plant-derived pheromone component, and the aggregation pheromone is mixed with the aggregation pheromone to enhance the effect. Preferably, the volume ratio of 2-undecyloxy-1-ethanol to farnesene is (6-70): 1; preferably (50-65):1, more preferably 60:1, for example 60 μm 2-undecyloxy-1-ethanol +1 μm farnesene.

In particular, in the present invention, the plant-derived pheromone and the aggregate pheromone are independently encapsulated, and it is preferable to control the distance between the plant-derived pheromone and the aggregate pheromone to 20cm or less; therefore, the plant source pheromone and the aggregation pheromone are prevented from being directly mixed to cause unnecessary reaction or cause that certain smell is completely covered by other smell and cannot play an effective effect, on the other hand, the plant source pheromone and the aggregation pheromone need to be matched for use, the space interval is kept to be less than or equal to 20cm, the plant source pheromone and the aggregation pheromone can be well fused in a space range to play a synergistic effect, and the generated effect is optimal. Preferably, the matching ratio of the plant source pheromone to the aggregation pheromone is 1: 1, namely 1 pack of plant source pheromone is matched with 1 pack of aggregation pheromone, and specifically, the mass ratio of the plant source pheromone to the aggregation pheromone is (20-30): (1-2), preferably (45-55): (2-4), more preferably 50: for example, the plant source pheromone is packaged in 1 pack of plant source pheromone by 50ml, and the aggregate pheromone is packaged in 1 pack of aggregate pheromone by 0.02-0.04ml, such as 60 microliter aggregate pheromone, and it should be noted that the volume is indicated for convenience of measurement, and the ratio of the two is based on the mass ratio.

In the invention, the aggregation pheromone is encapsulated in a capsule shell to prepare a capsule by preferably adopting a microcapsule embedding technology so as to improve the effective period of the attractant, and specifically, the capsule shell is made of animal and plant protein derivatives, and the thickness of the capsule wall is 0.5-10 mu m. Furthermore, the capsule formed by the gathered pheromone is dispersed in the cotton core body, and the cotton core body is plugged into the plastic pipe with at least one open end to form the lure core, so that the lure core has better durability. The length of the first sustained-release bag is preferably 8 +/-1 cm, the width is 1.4 +/-0.5 cm, the first sustained-release bag is a polyethylene bag, and the thickness of the first sustained-release bag is 20-300 μm, preferably 50-250 μm, more preferably 100-200 μm, such as 150 μm, such as 180 μm, such as 190 μm. The plastic pipe is 4 +/-1 cm long and 5 +/-0.5 mm in diameter.

In the invention, the plant source pheromone is released through a second slow release bag, wherein the second slow release bag is a polyethylene bag, and the thickness of the second slow release bag is 20-200 mu m; preferably 40-180 μm, more preferably 50-150 μm, such as 110 μm, such as 120 μm, such as 130 μm. For example, the length of the slow release bag is 13 + -0.5 cm, the width is 7 + -0.5 cm, the second slow release bag contains cotton, preferably absorbent cotton, 60-120ml of the plant source pheromone is added, and the bag is sealed hermetically.

In the invention, the adopted polyethylene slow-release bag and the inducing device are made of polyethylene into a film, the processing mode can be a casting process or a co-extrusion process, the density range of the raw material polyethylene is preferably 0.910-0.9708g/cm³Preferably in the range of 0.910 to 0.9408g/cm³. Compared with plastics made of other materials, the polyethylene has better attraction effect. The reason for this may be that this material is advantageous for the volatilization of pheromonesMeanwhile, the controlled release tablet has a certain controlled release function and prolongs the lasting period of the product.

The monochamus alternatus attractant provided by the invention is mainly improved in the formula, composition mode and use mode of plant source pheromone and aggregation pheromone, and other specific implementation modes such as a raw material mixing mode, a capsule embedding technology, a monochamus alternatus attractant placed in a trap, the adopted trap and the like can be the same as those in the prior art, so that the skilled person in the art can know the specific implementation modes and the adopted trap and the like, and the detailed description is not repeated.

Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available. In the following examples, "%" refers to volume percent, unless otherwise specified.

The luring device of the present invention is first detailed with reference to the accompanying drawings, referring to fig. 1 to 3, a monochamus alternatus luring device comprises a first slow release body 1 and a second slow release body 2, wherein the first slow release body 1 comprises a first slow release bag 11, a plastic tube 12 and a cotton core 13, a plurality of capsules 14 are absorbed in the cotton core 13, the capsules 14 store the aggregation pheromone 15, one end of the plastic tube 12 is open, and the two ends of the plastic tube 12 can also be open, the cotton core 13 is in a rod shape, the cotton core 13 is arranged in the plastic tube 12, the plastic tube 12 is arranged in the first slow release bag 11, and the mouth of the first slow release bag 11 is sealed; the second sustained release body 2 comprises a second sustained release bag 21, cotton 22 is arranged in the second sustained release bag 21, the cotton 22 adsorbs the plant source pheromone 23, and the mouth part of the second sustained release bag 21 is sealed; the first slow release body 1 and the second slow release body 2 are simultaneously arranged on a monochamus alternatus trap to release aggregation pheromone 15 and plant source pheromone 23.

Preferably, the capsule 14 is a protein derivative capsule, and the wall thickness of the capsule 14 is 0.5-10 μm.

Preferably, the plastic tube 12 is a polyethylene tube, and the wall thickness of the plastic tube 12 is 200-.

In a further improvement, a hanging through hole 111 is arranged on the sealed remaining section of the opening of the first slow release bag 11, and a hanging through hole 211 is arranged on the sealed remaining section of the opening of the second slow release bag 21. So that the first slow release bag 11 and the second slow release bag 21 are installed in a hanging manner.

Preferably, the first sustained-release bag 11 is a polyethylene bag with a thickness of 150-. The mouth of the first slow release bag 11 is sealed by heat sealing.

Preferably, the second sustained-release bag 21 is a polyethylene bag having a thickness of 20 to 220 μm. The mouth of the second sustained-release bag 21 is sealed by heat-sealing.

The mounting distance between the first slow release body 11 and the second slow release body 21 on the Monochamus alternatus trap is less than or equal to 20 cm.

The residual section of the first slow release bag 11 after the mouth part is sealed and the residual section of the second slow release bag 21 after the mouth part is sealed are detachably connected together.

The first slow release bag 11 and the second slow release bag 21 of the present embodiment may further be made of any one of a cast polypropylene film, a high temperature resistant PET polyester film, and a polyvinyl chloride/polyethylene composite film. The shape of the capsule 4 may be circular, cylindrical or oval.

The preparation method of the monochamus alternatus attractant comprises the following steps:

step 1: mixing turpentine, ethanol and acetaldehyde according to the volume percentage to form plant source pheromone liquid, filling a set amount of cotton 22 into the second slow release bag 21, adding a set amount of plant source pheromone liquid into the second slow release bag 21 to enable the plant source pheromone liquid to be absorbed into the cotton 22, and then sealing the opening of the second slow release bag 21 to prepare a second slow release body 2;

step 2: encapsulating the aggregation pheromone in a capsule shell by a microcapsule embedding technology to prepare capsules 14, adsorbing a plurality of the capsules 14 on a cotton core body 13, then installing the cotton core body 13 in a plastic pipe 12 with an opening at one end, then installing the plastic pipe 12 in a first sustained-release bag 11, and sealing the opening part of the first sustained-release bag 11 to prepare a first sustained-release body 1; the first and second sustained release bodies 1 and 2 may be prepared simultaneously or in any order.

And step 3: the residual section of the first slow release bag 11 after the mouth part is sealed and the residual section of the second slow release bag 21 after the mouth part is sealed are detachably connected together to form the attraction device.

As shown in fig. 4, the sealed remaining section of the mouth of the first slow release bag 11 and the sealed remaining section of the mouth of the second slow release bag 21 are connected by a staple 3 to form a detachable connection.

As shown in fig. 5, a hole 5 is punched at a position corresponding to the first slow release bag 11 and the second slow release bag 21, and then the first slow release bag 11 and the second slow release bag 21 are bound together by a connecting band 4 passing through the punched hole 5 to form a detachable connection.

The embodiment of the attracting device also provides the application of the monochamus alternatus attracting device, the attracting device is hung or placed in the monochamus alternatus trapper, and the attracting device releases monochamus alternatus attractant to trap monochamus alternatus into the monochamus alternatus trapper to be caught.

The following details examples of monochamus alternatus attractant and test results thereof.

Example 1, a plant source pheromone was prepared according to table 1, the components and volume ratios thereof remained unchanged, and the plant source pheromone consisted of the following components in volume percent: 36ml of turpentine, 18ml of ethanol and 6ml of acetaldehyde, then mixing the three components to form plant source pheromone liquid, then encapsulating the plant source pheromone liquid in a second slow release body according to the preparation method of the inducing device, and preparing ten second slow release bodies according to the same mode. One of the second sustained-release bodies is not paired with the following first sustained-release body, and the other nine are paired with one of the following first sustained-release bodies.

An aggregate pheromone was prepared according to A2-A5 of Table 1, and its component was 2-undecyloxy-1-ethanol. And divided into four different volumes: 0.04ml, 0.06ml, 0.08ml and 0.1ml, and then respectively encapsulated in the first sustained release bodies according to the preparation method to form four first sustained release bodies, wherein the four first sustained release bodies are respectively matched with one of the second sustained release bodies for use.

Another aggregate pheromone was prepared according to A6-A10 of Table 1 as a mixture of 2-undecyloxy-1-ethanol and farnesene. And is divided into five different volume ratios: 0.06ml of 2-undecyloxy-1-ethanol +0.5 mu m farnesene, 0.06ml of 2-undecyloxy-1-ethanol +1 mu m farnesene, 0.06ml of 2-undecyloxy-1-ethanol +3 mu m farnesene, 0.06ml of 2-undecyloxy-1-ethanol +5 mu m farnesene and 0.06ml of 2-undecyloxy-1-ethanol +10 mu m farnesene are respectively encapsulated in the first sustained release bodies to form five first sustained release bodies, and the five first sustained release bodies are respectively matched with one second sustained release body.

This results in ten pairwise applications, such as the formulation combinations shown in table 1. And finally, manufacturing five attracting devices in each matching mode for testing. The plastic pipes 12 of all the trapping devices are polyethylene pipes with the wall thickness of 300 μm; the first slow release bag 11 is a polyethylene bag with the thickness of 180 mu m; the second slow release bag 21 is a polyethylene bag with the thickness of 100 μm.

Table 1: different formula tables for 10 test groups

And then carrying out a trapping experiment, wherein the trapping experiment is carried out in forest farms in the United states of building, city, Fujian province, and the influence of the trapping effect on the trapping effect by matching the plant source pheromone with different addition amounts and different components under the condition of keeping the plant source pheromone unchanged is tested. The method is characterized in that 5 monochamus alternatus traps are arranged according to each formula in table 1 and matched with the formula for use, the first slow release bodies 11 and the second slow release bodies 21 are arranged on the monochamus alternatus traps at the same intervals, specifically 3cm, all the monochamus alternatus traps are of the same type and are randomly distributed, every two monochamus alternatus traps are spaced by 30-40m, the trapping period is 2019, 5 months, 9 days-6 months and 8 days, the number of monochamus alternatus trapped by each monochamus alternatus trap is recorded every week, then the trapping number of the 5 monochamus alternatus traps arranged in each formula test group is averaged, and the experimental result is shown in figure 6.

As can be seen from FIG. 6, when the pheromone component is 2-undecyloxy-1-ethanol, different additive amounts thereof have a significant effect on the trapping results, wherein the trapping effect of the formula A3 with the content of 60 microliters is the highest, and the trapping effect of the formula A2 with the content of 40 microliters is the lowest, while the trapping effect of the formula A1 which is not matched with the pheromone is the lowest, which indicates the importance of the matching use of the pheromone and the plant-derived pheromone, and the trapping effect can be significantly increased under a certain additive amount.

From the test results of formulation A6-A10, it was found that when the aggregation pheromone component was a mixture of 2-undecyloxy-1-ethanol and farnesene, it had a better trapping effect.

Example 2, the plant-derived pheromone is prepared according to the formula B1-B7 shown in table 2, the components are the same, the volume ratio is different, then the three components are respectively mixed to form plant-derived pheromone liquid, and the plant-derived pheromone liquid is encapsulated in the second sustained-release body according to the preparation method of the attraction device to form seven different second sustained-release bodies. Each second delay element is paired with a first delay element described below.

The plant source pheromone is prepared according to the components and the volume ratio of formula B8 in the table 2, and is encapsulated in the second sustained-release body according to the preparation method of the attraction device to form an eighth second sustained-release body, and the eighth second sustained-release body is matched with one first sustained-release body for use.

Aggregation pheromones were prepared according to formulations B1-B8 of table 2, the composition and volume of the aggregation pheromones were kept constant, and then encapsulated in the above-mentioned first sustained-release bodies to form eight first sustained-release bodies, which were used in a pair with one of the above-mentioned second sustained-release bodies of example 2, respectively.

This results in eight pairwise applications, such as the formulation combinations shown in table 2. And finally, manufacturing five attracting devices in each matching mode for testing. The plastic pipes 12 of all the trapping devices are polyethylene pipes with the wall thickness of 300 μm; the first slow release bag 11 is a polyethylene bag with the thickness of 180 mu m; the second slow release bag 21 is a polyethylene bag with the thickness of 100 μm.

Table 2 different formulations of the eight test groups

And then carrying out a trapping experiment, wherein the trapping experiment is carried out in a forest field of a gathering area in Xiamen city of Fujian province, and the influence of different volume ratios of the same components with the plant source pheromone or different paired components on the attracting effect of the monochamus alternatus hope is tested under the condition of keeping the aggregation pheromone unchanged. According to each formula of table 2, 5 monochamus alternatus traps are arranged to be matched with the formula for use, the first slow release bodies 11 and the second slow release bodies 21 are arranged on the monochamus alternatus traps at the same intervals, specifically 3cm, all the monochamus alternatus traps are of the same type and are randomly distributed, every two monochamus alternatus traps are spaced by 30-40m, the trapping period is 2019, 5 months, 9 days-6 months and 8 days, the number of monochamus alternatus trapped by each monochamus alternatus trap is recorded every week, then the trapping number of the 5 monochamus alternatus traps arranged in each formula test group is averaged, and the experimental result is shown in figure 7.

As can be seen from the attached figure 7, in the formulas B1-B6, different dosage ratios of turpentine and ethanol have significant influence on trapping results, wherein the trapping amount is the most when the formula B2, namely the ratio of the turpentine to the ethanol is 3:1, and the formula B1, namely the ratio of the turpentine to the ethanol is 6: the trapping amount is the minimum when 1, so whether the turpentine and the ethanol are reasonably matched is also important, and the experimental result shows that the optimal ratio of the turpentine to the ethanol is 3: 1.

Secondly, changing the proportion of the acetaldehyde content will also change the attraction effect, and from the formula B6-B8, the proportion of the turpentine and the ethanol is not changed, the proportion of the acetaldehyde is changed, and as can be clearly seen from the attached figure 7, the amount of the acetaldehyde which is not added and is added is too large, the number of the trapped acetaldehyde is small, the attraction effect is poor, the acetaldehyde is a synergistic substance, the content cannot be too high, through research and improvement, the acetaldehyde volume proportion does not need to exceed 10%, otherwise the effect is reduced, and even the reaction is generated.

Comparative example 1

Taking the formula of A8 in Table 1 as follows: 36ml of turpentine, 18ml of ethanol, 6ml of acetaldehyde, 0.06ml of 2-undecyloxy-1-ethanol and 3 mu m of farnesene, directly mixing the plant source pheromone and the aggregation pheromone, namely uniformly mixing 36ml of turpentine, 18ml of ethanol, 6ml of acetaldehyde, 0.06ml of 2-undecyloxy-1-ethanol and 3 mu m of farnesene to obtain a mixed solution, adding the mixed solution into a prepared slow release bag filled with absorbent cotton, wherein the slow release bag is a polyethylene bag with the thickness of 100 mu m, and the opening part of the slow release bag is thermally sealed to form the attracting device of the comparative example 1. The attracting device of comparative example 1 was made five by the same method.

The trapping test of monochamus alternatus was carried out by arranging the five trapping devices of comparative example 1 in 5 monochamus alternatus traps respectively, with the trapping test period ranging from 2019, 5 months, 9 days to 6 months, 8 days, recording the number of monochamus alternatus traps trapped by each monochamus alternatus trap every week, then averaging the trapping number of the 5 monochamus alternatus traps, and comparing the experimental results with the trapping results of the A8 test group of example 1, see particularly FIG. 8.

As can be seen from FIG. 8, the encapsulation mode of the plant-derived pheromone and the aggregation pheromone is only changed, the two are independently encapsulated in the formula A8 test group in example 1, and the trapping effect after direct mixing is obviously poor in comparative example 1. The reason is that the components of the plant source pheromone and the aggregation pheromone are different, the requirements on the volatilization rate are different, the components are directly mixed together in the comparative example 1 and are released through the slow release bag, the micromolecule substances are volatilized firstly, the macromolecule substances are volatilized later, the proportion of the formula in the later period is easy to be seriously unbalanced, the trapping effect is reduced, and meanwhile, the effective period is not long, so that the direct mixing of the two components is not feasible.

Comparative example 2

Taking the formula of A8 in Table 1 as follows: 36ml of turpentine, 18ml of ethanol, 6ml of acetaldehyde, 0.06ml of 2-undecyloxy-1-ethanol and 3 μm of farnesene were prepared, and 15 first controlled-release carriers (containing collectin) and 15 second controlled-release carriers (containing plant-derived pheromones) were prepared in the same manner as in example 1.

Then 5 first slow release bodies and 5 second slow release bodies are bound together to form five luring devices, the five luring devices are respectively arranged in five monochamus alternatus traps to form a first test group, the other five first slow release bodies and the five second slow release bodies are correspondingly arranged in the five monochamus alternatus traps at 18cm intervals to form a second test group, the other five first slow release bodies and the five second slow release bodies are correspondingly arranged in the five monochamus alternatus traps at 25cm intervals to form a third test group, then the three test groups are simultaneously used for carrying out trapping tests, fifteen monochamus alternatus traps are randomly distributed, the interval between every two monochamus alternatus traps is 30-40m, the trapping test period is 2019, 5, month 9, day-6, month 8, the number of monochamus alternatus traps of each monochamus alternatus trap is recorded every week, the number of traps in each group was averaged and the results are shown in FIG. 9.

As can be seen from FIG. 9, the trapping effect of the first test group is the best, and the trapping effect of the second test group is the second but not very different; the third test group had the worst and significantly different trapping effects. From this experiment, it can be shown that the distance between the first sustained release body and the second sustained release body cannot be too large, and should be ≦ 20cm, otherwise the trapping effect will be affected.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention. The invention is not described in detail in order to avoid unnecessary repetition.

In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.

Claims

1. The Monochamus alternatus attractant is characterized in that: the attractant comprises plant source pheromone and aggregation pheromone, wherein the plant source pheromone and the aggregation pheromone are mutually synergistic to attract monochamus alternatus;

2. The Monochamus alternatus attractant according to claim 1, wherein: the mass ratio of the plant source pheromone to the aggregation pheromone is (20-30): (1-2).

3. The Monochamus alternatus attractant according to claim 1, wherein: the plant source pheromone comprises the following components in percentage by volume: 55-75% of turpentine, 15-35% of ethanol and 6-9% of acetaldehyde;

optionally, the volume ratio of turpentine to ethanol in the plant source pheromone is (1.5-4) to 1;

optionally, the volume ratio of turpentine, ethanol and acetaldehyde in the plant source pheromone is (5.5-6.5): (2.5-3.5): (0.5-1.5).

4. The Monochamus alternatus attractant according to any one of claims 1 to 3, wherein: the aggregation pheromone further comprises farnesene, and the farnesene and the 2-undecyloxy-1-ethanol are mixed with each other.

5. The Monochamus alternatus attractant according to claim 4, wherein: the volume ratio of the 2-undecyloxy-1-ethanol to the farnesene in the aggregation pheromone is (6-70): 1; more preferably (50-65): 1.

6. The utility model provides a pine black longicorn trapping apparatus which characterized in that: the first sustained release body comprises a first sustained release bag, a cotton core is filled in the first sustained release bag, a plurality of capsules are absorbed in the cotton core, the aggregation pheromone of any one of claims 1 to 5 is stored in the capsules, and the mouth part of the first sustained release bag is sealed; the second slow release body comprises a second slow release bag, cotton is filled in the second slow release bag, the plant source pheromone in any one of claims 1 to 5 is adsorbed on the cotton, and the opening part of the second slow release bag is sealed; the first slow release body and the second slow release body are simultaneously arranged on a monochamus alternatus trap to release aggregation pheromone and plant source pheromone.

7. The Monochamus alternatus luring device of claim 6, wherein: the cotton core body is arranged in a plastic pipe with at least one open end, and the plastic pipe is arranged in the first slow release bag;

the mounting distance between the first slow release body and the second slow release body on the Monochamus alternatus trap is less than or equal to 20 cm.

8. The Monochamus alternatus luring device of claim 7, wherein: the first slow release bag is made of polyethylene, and the density range of the polyethylene is 0.910-0.9708g/cm³The wall thickness of the first slow release bag is 150-;

the second slow release bag is made of polyethylene, and the density range of the polyethylene is 0.910-0.9708g/cm³(ii) a The wall thickness of the second slow release bag is 20-200 mu m;

the plastic pipe is a polyethylene pipe, and the wall thickness of the plastic pipe is 200-400 mu m;

the residual section after the first slow release bag opening is sealed is detachably connected with the residual section after the second slow release bag opening is sealed.

9. A method for preparing the monochamus alternatus luring device as defined in any one of claims 6 to 8, wherein the method comprises the following steps: the method comprises the following steps:

step 2: encapsulating the aggregation pheromone in a capsule shell by a microcapsule embedding technology to prepare capsules, adsorbing a plurality of the capsules on a cotton core body, then arranging the cotton core body in a plastic tube with at least one open end, arranging the plastic tube in a first sustained-release bag, and sealing the opening part of the first sustained-release bag to prepare a first sustained-release body; the first slow release body and the second slow release body can be prepared simultaneously or in any arrangement sequence;

10. Use of the monochamus alternatus luring device as defined in any one of claims 6 to 8, wherein: the attraction device is hung or placed in the monochamus alternatus trap, and releases monochamus alternatus attractant to trap monochamus alternatus into the monochamus alternatus trap to be caught.