CN112956479B - Insect attractant and controlled release system and application thereof - Google Patents
Insect attractant and controlled release system and application thereof Download PDFInfo
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- CN112956479B CN112956479B CN202110152329.XA CN202110152329A CN112956479B CN 112956479 B CN112956479 B CN 112956479B CN 202110152329 A CN202110152329 A CN 202110152329A CN 112956479 B CN112956479 B CN 112956479B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
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- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides an insect attractant, a controlled release system and application thereof. The invention discloses the application of Z, 3hexenyl Z, 3hexenoate as an attractant for cnaphalocrocis medinalis for the first time. The Z, 3hexenyl Z, 3 hexenoic acid salt has the attraction characteristic to the pests such as the female and the male rice leaf roller in the field, has obvious attraction effect, can accurately attract and kill the female and the male adult rice leaf roller, reduces the number of field adults, and controls the insect pest of the rice leaf roller from the source. The invention has no harm to natural enemies, beneficial insects, human beings, livestock and crops, and target pests have no resistance, thus having the advantages of naturalness, environmental protection and the like.
Description
Technical Field
The invention relates to the field of crop pest control, in particular to an insect attractant, a controlled release system and application thereof.
Background
Rice is the most important agricultural crop in the world, and the protection of the crop from diseases and pests is an important link for maintaining the grain yield and ensuring the grain safety. Control of rice pests can be achieved by spraying insecticides, but toxicity to users, residues on crops and environmental pollution are major problems when using insecticides. Which in turn leads to insecticide resistance development and failure of prevention and control. In addition, destruction of natural enemies by broad spectrum insecticides can lead to a proliferation of secondary pests that are resistant to the insecticides and become as serious as the primary pests when their natural enemies are destroyed. Therefore, selective measures are required to control major pests while not damaging natural enemies, to ensure high yield of rice. The major pests on rice include Chilo suppersalis (Chilo suppersalis), Scirpus suppressalis (Scirphapoga incertulas), Sesamia inferens (Sesamia inferens), and Cnaphalocrocis medinalis (Cnaphalocrocis medinalis). The first two borers have weak migratory flight ability and are usually bred locally. The sesamia inferens has certain migration capacity, but the migration distance is very limited. The rice leaf rollers migrate from Burmese and Thailand to the rice growing region in China for thousands of kilometers every year. Sex pheromones may be used as attractants in trapping devices to reduce the number of males, thereby limiting the number and egg production of fertilized eggs. The technology has the characteristics of high selectivity, no toxin, no harm to beneficial insects, no resistance, no residue in crops and environment and the like. It is effective against those species that do not migrate or are primarily indigenous harmful. The large scale trapping or disruption of mating using pheromones is not applicable to cnaphalocrocis medinalis, as many foreign female moths have mated. However, the long distance flight is energy consuming, so the rice leaf roller has strong power to search sugar source from nectar. Because most of the activity time of the borers is after dark, the rice leaf rollers find the flowers through volatile fragrance emitted by the flowers. The number of eggs laid by female moths fed with nectar is more than that of the same kind fed with nectar. The greater borer has no strong migration ability of the cnaphalocrocis medinalis, but can also supplement energy by eating nectar. Other species such as Chilo suppressalis and Chilo suppressalis, which do not require long-distance migration, do not have strong power to look for honey sources, and have no obvious attraction to flower volatiles.
Flowering plants produce a wide variety of flower volatiles. They have evolved to attract insects to pollinate. Similarly, moths have evolved to look for honey sources through flower aroma. Different plant volatiles attract different kinds of insects. Some insects react to a single compound, but most insects require a mixture of volatiles to be clearly attractive. Some plant volatiles may not be effective attractants to target pests and may even be repellent agents. If a mixture of floral volatiles could be identified that is attractive to a particular pest, it would be potentially very useful in "attract and kill" mass trapping, reducing not only farmers' labor, but also pesticide requirements for rice crops.
Disclosure of Invention
The invention aims to provide an insect attractant, a controlled release system and application thereof.
The inventor unexpectedly finds that Z, 3hexenyl Z, 3hexenoate, an unusual flower volatile, has strong attraction to rice leaf rollers and can be used as an insect attractant.
The structure of Z, 3HeXenyl Z, 3Hexenoate (Z, 3HeXenyl Z, 3Hexenoate) is as follows:
to achieve the object of the present invention, in a first aspect, the present invention provides any one of the following uses of Z, 3hexenyl Z, 3 hexenoic acid salt:
1) as an insect attractant;
2) for attracting insects.
In the present invention, the insects include crop pests including, but not limited to, rice leaf rollers.
In a second aspect, the present invention provides a controlled release system for Z, 3hexenyl Z, 3hexenoate, said controlled release system being selected from any one of the following (i) to (iii):
firstly, a controlled release capsule, a controlled release bag, a controlled release tube or a controlled release bottle: a capsule, bag, tube or bottle containing Z, 3hexenyl Z, 3 hexenoate;
② sustained-release granules; a particulate inert carrier mixed with or adsorbed with Z, 3hexenyl Z, 3 hexenoate;
③ a controlled-release capsule, a controlled-release bag, a controlled-release tube or a controlled-release bottle which is filled with the mixture of Z, 3hexenyl Z, 3 hexenoic acid salt and oil material.
The material of the controlled release capsule, the controlled release bag, the controlled release tube or the controlled release bottle is permeable thermoplastic polymer, and is selected from one or more of high density polyethylene, low density polyethylene, ethylene vinyl acetate, thermoplastic polyurethane, polyvinyl chloride and the like.
The inert carrier may be paraffin wax, microcrystalline wax, beeswax, wax base, talc or rubber granules.
Optionally, the controlled release system is formed by mixing Z, 3hexenyl Z, 3hexenoate with paraffin, talcum powder or rubber particles according to the weight ratio of 1:1-1: 10.
In a preferred embodiment of the invention, the controlled release system is formed by mixing Z, 3hexenyl Z, 3hexenoate with paraffin in a weight ratio of 1: 4 or 1: 9.
In a preferred embodiment of the invention, the controlled release system is formed by mixing Z, 3hexenyl Z, 3hexenoate with rubber particles in a weight ratio of 1: 9 to 1: 4.
Optionally, the controlled release system is obtained by mixing Z, 3hexenyl Z, 3 hexenoic acid salt and oil according to the volume ratio of 1:50-1:2, and then filling the mixture into a controlled release capsule, a controlled release bag, a controlled release tube or a controlled release bottle.
Wherein the oil can be at least one selected from rapeseed oil, olive oil, corn oil, etc.
In a preferred embodiment of the invention, the controlled release system is obtained by mixing Z, 3hexenyl Z, 3hexenoate with rapeseed oil in a volume ratio of 1: 19, 1: 9 or 1: 4 and then filling the mixture into a controlled release capsule, a controlled release bag, a controlled release tube or a controlled release bottle.
In a third aspect, the present invention provides the use of said controlled release system in crop pest trapping.
In a fourth aspect, the invention provides a method for controlling crop pests, wherein the controlled release system containing Z, 3hexenyl Z, 3hexenoate is placed in an insect trapping device and placed in a field to attract pests to gather and perform centralized killing.
By the technical scheme, the invention at least has the following advantages and beneficial effects:
the invention discloses the application of Z, 3hexenyl Z, 3hexenoate as a phagostimulant (attractant) of cnaphalocrocis medinalis for the first time. The Z, 3hexenyl Z, 3 hexenoic acid salt has the attraction characteristic to the female pests of the rice leaf roller and the like in the field, has obvious attraction effect, can accurately attract and kill the female and male adults of the rice leaf roller, reduces the number of field adults, and controls the insect pests of the rice leaf roller from the source. The invention has no harm to natural enemies, beneficial insects, human beings, livestock and crops, and target pests have no resistance, thus having the advantages of naturalness, environmental protection and the like.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
The Z, 3hexenyl Z, 3hexenoate salts used in the examples below were purchased from the shanghai meirell chemical technology co.
Example 1 attraction of Z, 3hexenyl Z, 3 hexenoic acid salts to Cnaphalocrocis medinalis
This example utilizes Z, 3hexenyl Z, 3hexenoate to attract male and female rice leaf rollers and incorporates a controlled release device for trapping and controlling the pest.
1.Z, 3hexenyl Z, 3hexenoate was added to a controlled release device consisting of a 10ml dropper bottle of low density polyethylene (available from package materials factory for rehabilitation, Canon, Hebei province, model DP3A-10) in amounts of 0.1, 1.0, 2.0, 5.0 and 10ml per bottle. In paddy fields in certain places of Jiangsu province in 6 months, a commercial snout moth trapper is matched for testing, and a set of trapping devices are placed every 20 meters. After 4 days, the attracted rice leaf roller imagoes are collected, and the species and the sex of the rice leaf roller imagoes are determined. The control is selected to match with a commercial rice leaf roller sex pheromone lure core (purchased from Beijing Zhongjie Site Biotech GmbH) and a commercial snout moth trapper for testing. The results are shown in Table 1.
TABLE 1 attraction effect of low-density polyethylene bottle as slow-release carrier (head)
From table 1 it can be seen that within certain limits, Z, 3hexenyl Z, 3 hexenoic acid salts are all effective. Each carrier is 1.0, 2.0 and 5.0mL, and the attraction effect is obviously better than other dosage. Namely, 1.0-5.0 mL of Z, 3hexenyl Z and 3hexenoate is used as each bait, and the attraction capacity is strongest.
2. To demonstrate the effectiveness of other controlled release vehicles, Z, 3hexenyl Z, 3hexenoate and molten paraffin were combined at a weight ratio of 1: 9, 1: 4, 1:1, respectively, and 5 grams were dispensed into disks. Each bait formulation contained 0.5 grams, 1.0 grams, 2.5 grams of active ingredient. The bait is placed in a snout moth trapper, and the result shows that the Z, 3hexenyl Z, 3 hexenoic acid salt bait prepared by the method has an attraction effect on rice leaf rollers. The control is selected to match with a commercial rice leaf roller sex pheromone lure core (purchased from Beijing Zhongjie Site Biotech GmbH) and a commercial snout moth trapper for testing. After 4 days, the lured rice leaf roller imagoes are collected, and the species and sex of the rice leaf roller imagoes are determined. The results are shown in Table 2.
TABLE 2 attractant effect of wax carrier as slow release carrier (head)
It can be seen that the wax formulation is an effective method for releasing the salt of Z, 3hexenyl Z, 3 hexenoic acid, thereby trapping rice leaf rollers. The attracting effect of the treatment group in which Z, 3hexenyl Z, 3 hexenoic acid salt and molten paraffin are mixed in a weight ratio of 1: 4 and 1: 9 is better than that of the treatment group in which the salt and the molten paraffin are mixed in a weight ratio of 1:1, but the attracting effect of the treatment group in which the salt and the molten paraffin are mixed in a weight ratio of 1:1 is still better than that of the commercial lure.
3. It was further demonstrated that another controlled release system, which can effectively attract adult male and female rice leaf rollers, was prepared by heat-sealing 20 mL of a mixture of Z, 3hexenyl Z, 3 hexenoic acid salt and rapeseed oil (Z, 3hexenyl Z, 3 hexenoic acid salt and rapeseed oil were mixed at a volume ratio of 1: 49, 1: 19, 1: 9, 1: 4, respectively) in a PVC tube (internal diameter 2.0 cm, length 10 cm, wall thickness 0.8 mm), wherein the content of Z, 3hexenyl Z, 3 hexenoic acid salt in each controlled release device was 0.4mL, 1.0mL, 2.0mL, 4mL, respectively. The control is tested by matching a commercial rice leaf roller sex pheromone lure core with a commercial snout moth trapper. The bait is placed in a snout moth trapper, and the trapped rice leaf roller imagoes are collected after 4 days, and the types and the sexes of the rice leaf roller imagoes are determined. The results are shown in Table 3.
TABLE 3 attraction effect of Cnaphalocrocis medinalis phagostimulant with PVC/vegetable oil as slow-release carrier (first)
The results show that the PVC/vegetable oil formulation is an effective method for the release of Z, 3hexenyl Z, 3 hexenoate. Wherein the luring effect of the mixture of Z, 3hexenyl Z, 3hexenoate and rapeseed oil according to the volume ratio of 1: 19, 1: 9 and 1: 4 is better for the rice leaf roller imagoes, but the luring effect of the mixture according to the volume ratio of 1: 49 is still better than that of the commercial sex attractant.
4. Another expression is a granular carrier, which uses rubber particles (type: XJKL-GHJ6, particle size 1-2mm, from the manufacturer of Textken mineral products, Lingshou, Ling) as the carrier for Z, 3hexenyl Z, 3 hexenoic acid salt. Z, 3hexenyl Z, 3hexenoate and rubber particles (recycled tire rubber) were mixed in a weight ratio of 1: 9, 1: 4, 1:1, respectively, and 50 grams of the mixture was placed on the bottom of barrel traps, each corresponding to 5.0 grams, 10.0 grams, 25.0 grams of Z, 3hexenyl Z, 3hexenoate, respectively. The control is selected to match with the sex pheromone lure core of the rice leaf roller sold in the market and the rice moth trapper sold in the market for testing, and the test result after 4 days is shown in the table 4.
TABLE 4 attraction effect of Cnaphalocrocis medinalis phagostimulant on adsorption of Z, 3hexenyl Z, 3hexenoate by granular rubber (first)
The result shows that the rubber particles are used as the carrier, can effectively release Z, 3hexenyl Z, 3 hexenoic acid salt and has good attraction effect on the rice leaf rollers. The attracting effect of the treatment group in which the Z, 3hexenyl Z, 3 hexenoic acid salt and the rubber particles are mixed according to the weight ratio of 1: 9 to 1: 4 is better than that of the treatment group in which the rubber particles are mixed according to the weight ratio of 1:1, but the attracting effect of the treatment group in which the rubber particles are mixed according to the weight ratio of 1:1 is still better than that of the commercial sex attractant.
5. Another method for preparing Z, 3hexenyl Z, 3 hexenoic acid salt is to use powder as carrier. Talc was used as a carrier for the Z, 3hexenyl Z, 3 hexenoic acid salt. Z, 3hexenyl Z, 3hexenoate and talc were mixed in a weight ratio of 1: 4, 100 grams were placed at the bottom of the traps, corresponding to 20 grams of Z, 3hexenyl Z, 3hexenoate per trap. The control is selected to match with a commercial rice leaf roller sex pheromone lure core (purchased from Beijing Zhongjie Site Biotech GmbH) and a commercial snout moth trapper for testing. After 4 days, the rice leaf roller imagoes are collected, and the species and sex of the rice leaf roller imagoes are determined. The results are shown in Table 5.
TABLE 5 attraction effect of Cnaphalocrocis medinalis phagostimulant with talc powder as carrier to adsorb Z, 3hexenyl Z, 3hexenoate (first)
| Treatment of | 20.0 g/lure core | Control |
| Repetition of 1 | 6 | 0 |
| Repetition 2 | 3 | 2 |
| Repetition of 3 | 8 | 0 |
| Repetition of 4 | 2 | 0 |
| Repetition 5 | 4 | 0 |
| Total of | 23(10 female, 13 male) | 2 (Male worm) |
Although the release system can still attract the cnaphalocrocis medinalis imagoes, the attraction efficiency is lower compared with other release methods, probably because the release rate of the attractant is too fast by the release system, and the too high concentration has the evasive effect on the cnaphalocrocis medinalis imagoes.
From the above studies, it can be concluded that Z, 3hexenyl Z, 3hexenoate has an attractive effect on both female and male cnaphalocrocis medinalis and that a range of different delivery systems are effective. These experiments show that in different release systems, Z, 3hexenyl Z, 3hexenoate can attract rice leaf rollers to enter the trap.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
- Use of any of the following Z, Z-3-hexenyl esters:1) as an insect attractant;2) for attracting insects;the insects are crop pests, and the crop pests are rice leaf rollers.
- The application of a Z, Z-3-hexenoic acid-3-hexenyl ester controlled release system in trapping crop pests; the crop pests are rice leaf rollers, and the controlled release system is selected from any one of the following materials:firstly, a controlled release capsule, a controlled release bag, a controlled release tube or a controlled release bottle: a capsule, bag, tube or bottle containing Z, Z-3-hexenyl-3-enoate;② sustained-release granules; a particulate inert carrier mixed with or adsorbed with Z, Z-3-hexenyl ester;③ controlled-release capsules, controlled-release bags, controlled-release tubes or controlled-release bottles filled with the mixture of Z, Z-3-hexenoic acid-3-hexenyl ester and oil materials.
- 3. The use of a controlled release system of Z, Z-3-hexenoic acid-3-hexenyl ester according to claim 2 in crop pest trapping wherein the material of the controlled release capsule, bag, tube or bottle is a permeable thermoplastic polymer selected from at least one of high density polyethylene, low density polyethylene, ethylene vinyl acetate, thermoplastic polyurethane, polyvinyl chloride.
- 4. The use of a controlled release system of Z, Z-3-hexenoic ester according to claim 2 in the trapping of crop pests characterized in that said granular inert carrier is paraffin wax, microcrystalline wax, beeswax, talc or rubber granules.
- 5. The use of a controlled release system of Z, Z-3-hexenoic-3-hexenyl ester according to claim 2 in crop pest trapping, characterized in that the controlled release system is made by mixing Z, Z-3-hexenoic-3-hexenyl ester with paraffin, talc or rubber particles in a weight ratio of 1:1 to 1: 10.
- 6. The use of the controlled release system of Z, Z-3-hexenoic-3-hexenyl ester according to claim 2 in crop pest trapping, characterized in that it is obtained by mixing Z, Z-3-hexenoic-3-hexenyl ester and oil in the volume ratio of 1:50-1:2 and then filling it into controlled release capsules, controlled release bags, controlled release tubes or controlled release bottles.
- 7. The use of a controlled release system of Z, Z-3-hexenoic ester according to claim 6 in pest trapping of crops, wherein said oil is selected from at least one of rapeseed oil, olive oil, corn oil.
- 8. A method for controlling cnaphalocrocis medinalis, characterized in that the controlled release system according to any one of claims 2 to 7 is placed in an insect trapping device, placed in the field, lures cnaphalocrocis medinalis to gather and carries out centralized killing.
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