CN109907075B - Trapping and killing composition for preventing and controlling small beetles in beehives and application method - Google Patents
Trapping and killing composition for preventing and controlling small beetles in beehives and application method Download PDFInfo
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- CN109907075B CN109907075B CN201910191069.XA CN201910191069A CN109907075B CN 109907075 B CN109907075 B CN 109907075B CN 201910191069 A CN201910191069 A CN 201910191069A CN 109907075 B CN109907075 B CN 109907075B
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Abstract
The invention discloses a trapping and killing composition for preventing and controlling beehive beetles and an application method thereof. The trapping and killing composition for preventing and treating the beetles in the honeycomb comprises, by mass, 60-80 parts of honeycomb powder, 1-5 parts of bee pollen, 1-5 parts of yeast powder, 5-10 parts of glycerol, 10-20 parts of water and 1-2 parts of efficient cyhalothrin or 2-4 parts of chlorpyrifos. The trapping and killing composition for preventing and controlling the beetles in the beehive utilizes the slow-release substance with special smell to prepare the trapping and killing agent, attracts a large amount of the small beetles in the honeycomb into the trapping and killing bottle coated with the trapping and killing composition, and utilizes the insecticide to kill the small beetles in the honeycomb, so that the bee honeycomb is protected from being damaged by the small beetles in the honeycomb. The trapping and killing composition has the characteristics of low cost, high efficiency, environmental friendliness and the like. The trapping and killing composition for the small bee hive beetles and the application thereof have wide industrialization prospect, are particularly suitable for preventing and controlling the small bee hive beetles in bee hives, and have wide economic and social benefits.
Description
Technical Field
The invention belongs to the technical field of agricultural pest control, and particularly relates to a trapping and killing composition for controlling beehive beetles and an application method thereof.
Background
The Beehrla nidus Aethina tubida Murray belongs to Coleoptera, Nitidulidae, and is one of six important pathogens of bees. It was originally distributed in the African continent southward in the Sahara desert, and in 1998, Beatles beetles were first discovered in the Florida states of the United states (Hepburn and Radloff,1998) and by 2002 have spread to 25 states, causing serious losses to the beekeeping industry in the United states. In recent years, it has been spread successively to Egypt (2000), Canada (2002), Australian (2002), Italy (2014) and Korea (2016) countries and regions (Neumannet al, 2016; Toufailia et al, 2017; Leeet al, 2017). And it is known that beetles of the honeycomb are found in some parts of China.
The bee hive beetle is a parasitic insect in bee colony, the insect has wide spread route, strong viability and fast reproduction speed, and the adult insect feeds on honey, pollen and even bee larva to cause the death of bee larva and larva, and can still survive for more than 5 days without food (Hufuliang and Zhuwei, 2002). When the larva of the small beetle in the honeycomb digs into a hole and passes through the honeycomb to eat the honey and pollen in the honeycomb, the part where the larva passes is damaged, the whole honeycomb collapses and is accompanied by a fermentation phenomenon, the honey foams and overflows the honeycomb, even flows out of a beehive, and the quality of the bee product is seriously affected (soldiers in the army, 2005). In addition, the beetle larvae of the hive often leave a sticky substance with odor and emit an odor similar to rotten oranges, forcing the bee colony to abandon the hive and escape (Zhao Hongyao et al, 2018). Once a colony is heavily infected, either a strong or weak colony, it is destroyed within two weeks.
The prevention and treatment of the Beatles nidulans, until now, no method for thoroughly radically treating the Beatles nidulans exists. The chemical agent control methods that can be used are: U.S. medicine "checkmate +" for beehives, which checks for beetle larvae in the hive; the drug "Gardsar" used for treating soil is used for interrupting the breeding cycle of the Beatles nidulans and preventing the spread of the Beatles nidulans. However, such trapping compositions and applications are currently lacking.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a trapping and killing composition for preventing and controlling the small beetles in honeycomb with low cost, high efficiency and environmental friendliness and an application method thereof.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the trapping and killing composition for preventing and treating the beetles in the honeycomb comprises, by mass, 60-80 parts of honeycomb powder, 1-5 parts of bee pollen, 1-5 parts of yeast powder, 5-10 parts of glycerol, 10-20 parts of water and 1-2 parts of efficient cyhalothrin or 2-4 parts of chlorpyrifos.
Preferably, the trapping and killing composition for controlling the beetles on the honeycomb comprises, by mass, 60-80 parts of honeycomb powder, 1-5 parts of bee pollen, 1-5 parts of yeast powder, 5-10 parts of glycerol, 10-20 parts of water and 5-10 parts of 20% of efficient cyhalothrin or 40% of chlorpyrifos.
Preferably, the trapping and killing composition for controlling the beetles on the honeycomb comprises, by mass, 70 parts of honeycomb powder, 2 parts of bee pollen, 2 parts of yeast powder, 10 parts of glycerol, 10 parts of water, and 6 parts of 20% efficient cyhalothrin or 40% chlorpyrifos.
The second purpose of the invention is to provide a method for trapping and killing the small bee hive beetles, which is characterized in that the trapping and killing composition for preventing and controlling the small bee hive beetles is put into a trapping and killing bottle, and is put near a bee hive to trap and kill the small bee hive beetles with poison, and the trapping and killing bottle is provided with a plurality of holes for the small bee hive beetles to enter on the wall of the bottle.
Preferably, the trapping and killing bottle is a polypropylene plastic empty bottle, and a plurality of holes for the honeycomb beetles to enter are arranged on the trapping and killing bottle.
Preferably, the size of the hole on the trapping bottle is 3.5mm long (long axis) and 2.0mm wide (short axis) (chest width of worker bee in bee >3.5mm), so that the bee is prevented from entering the trapping bottle through the trapping hole while the bee larva can smoothly enter the trapping bottle, and the bee is prevented from being injured.
According to the invention, the strong tropism of the small honeycomb beetles to the trapping and killing composition is utilized to prepare the trapping and killing agent, so that a large number of small honeycomb beetles are trapped and gathered in the trapping and killing bottle in which the trapping and killing composition is laid, and the small honeycomb beetles die due to contact of pesticides on the body surface.
The invention has the advantages that: the trapping and killing composition for preventing and controlling the beetles in the beehive utilizes the slow-release substance with special smell to prepare the trapping and killing agent, attracts a large amount of the small beetles in the honeycomb into the trapping and killing bottle coated with the trapping and killing composition, and utilizes the insecticide to kill the small beetles in the honeycomb, so that the bee honeycomb is protected from being damaged by the small beetles in the honeycomb. The trapping and killing composition has the characteristics of low cost, high efficiency, environmental friendliness and the like. The trapping and killing composition for the small bee hives and the application thereof have wide industrialization prospect, the preparation method is simple, and the composition is particularly suitable for preventing and controlling the small bee hives in bee hives and has wide economic and social benefits.
Drawings
FIG. 1 is a graph of the anova of different formulations of the trap composition for controlling Beehive beetles.
FIG. 2 is a schematic representation of the application of the trapping and killing composition for controlling Beatles nidus.
Reference numerals: 1-trapping and killing bottle; 2-elliptical aperture; 3. a trapping and killing composition for preventing and treating the beetles in beehives.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer and clearer, the present invention is further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The 40% chlorpyrifos is a 40% chlorpyrifos emulsifiable concentrate containing 400g chlorpyrifos per liter, a commercially available product.
20 percent (mass fraction) of high-efficiency cyhalothrin is a commercial product, and the high-efficiency cyhalothrin is called as cyhalothrin and cyhalothrin.
Example 1:
the trapping and killing composition for preventing and controlling the honeycomb beetles comprises the following formulas which are respectively prepared from the following raw materials in parts by weight:
formula 1: 74 parts of honeycomb powder, 10 parts of glycerol, 10 parts of water and 6 parts of 40% chlorpyrifos.
And (2) formula: 74 parts of bee pollen, 10 parts of glycerol, 10 parts of water and 6 parts of 40% chlorpyrifos.
And (3) formula: 72 parts of bee pollen, 2 parts of dry yeast powder (namely dry yeast, the same below), 10 parts of glycerol, 10 parts of water and 6 parts of 40% chlorpyrifos;
and (4) formula: 70 parts of honeycomb powder, 2 parts of bee pollen, 2 parts of dry yeast powder, 10 parts of glycerol, 10 parts of water and 6 parts of 40% chlorpyrifos.
And (5) formula: 74 parts of dry yeast powder, 10 parts of glycerol, 10 parts of water and 6 parts of 40% chlorpyrifos.
The preparation method comprises the steps of mixing the components according to the content of the components in the formula.
The trapping and killing composition 3 for preventing and controlling the small beetles in the honeycomb according to the formula 1-5 is laid in the trapping and killing bottle 1. The trapping bottle 1 is made of a common empty bottle, the size of an opening on the empty bottle can be determined according to the width and the thickness of the chest of the small beetle in the honeycomb, and the selection of the material of the application device is preferred to be low in cost. Adopt common polypropylene plastics empty bottle 1 to make in this embodiment, be equipped with many holes 2 on the empty bottle wall, the hole size is long (major axis) 3.5mm, and wide (minor axis) 2.0 mm's elliptical aperture (the chest width of worker's bee in the honeybee >3.5mm), when guaranteeing that the little beetle of honeycomb can get into smoothly and trap and kill the bottle, prevent that the honeybee from getting into and trapping and killing the bottle through trapping and killing the aperture to avoid causing the injury to the honeybee. In the adult stage of the small bee hive beetles, the trapping bottles coated with the trapping and killing composition for preventing and controlling the small bee hive beetles are placed at the bottom of the beehive, and the number of the trapping and killing bottles can be properly adjusted to about 1-2 according to the size of the insect population density. After the small beetles are trapped in the trapping bottles in which the trapping and killing composition is laid, 20% of efficient cyhalothrin or 40% of chlorpyrifos is contacted with the body surface to kill the beetles.
Selecting 15 groups of bees parasitized by the bee hive beetles (each formula is 3 groups of bees to carry out a parallel test), adding 5 formulas into the trapping and killing bottle respectively under the condition of determining that the parasitization rates are consistent, then placing the test bee groups into the trapping and killing bottle for trapping and killing, keeping for 48 hours, and counting the test results, wherein the results are shown in figure 1. By comparing the 5 formulations of the beetle bee trap composition, it was found that formulation 4 had a significant insecticidal effect and a significant synergistic effect (see fig. 1), and that formulation 5 was completely non-attractive to the beetles bee hive (as no effect, not shown).
Example 2:
the trapping and killing composition for controlling the beetles on the honeycomb comprises, by mass, 60 parts of honeycomb powder, 1 part of bee pollen, 1 part of dry yeast powder, 5 parts of glycerol, 10 parts of water and 5 parts of 20% cyhalothrin, and the components are uniformly mixed according to the content of the components. The trapping and killing composition for preventing and treating the small bee hive beetles has good trapping and poisoning effects on the small bee hive beetles.
Example 3:
the trapping and killing composition for controlling the beetles in the hive comprises, by mass, 60 parts of hive powder, 1 part of bee pollen, 1 part of dry yeast powder, 5 parts of glycerol, 10 parts of water and 5 parts of 40% chlorpyrifos, and the components are uniformly mixed according to the content of the components. The trapping and killing composition for preventing and treating the small bee hive beetles has good trapping and poisoning effects on the small bee hive beetles.
Example 4:
the trapping and killing composition for controlling the beetles on the honeycombs comprises, by mass, 80 parts of honeycomb powder, 5 parts of bee pollen, 5 parts of dry yeast powder, 10 parts of glycerol, 20 parts of water and 10 parts of 20% cyhalothrin, and the components are uniformly mixed according to the content of the components. The trapping and killing composition for preventing and treating the small bee hive beetles has good trapping and poisoning effects on the small bee hive beetles.
Example 5:
the trapping and killing composition for controlling the beetles in the hive comprises, by mass, 80 parts of hive powder, 5 parts of bee pollen, 5 parts of dry yeast powder, 10 parts of glycerol, 20 parts of water and 10 parts of 40% chlorpyrifos, and the components are uniformly mixed according to the content of the components. The trapping and killing composition for preventing and treating the small bee hive beetles has good trapping and poisoning effects on the small bee hive beetles.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.
Claims (4)
1. The trapping and killing composition for preventing and treating the beetles on the honeycomb is characterized by comprising 70 parts of honeycomb powder, 2 parts of bee pollen, 2 parts of yeast powder, 10 parts of glycerol, 10 parts of water and 6 parts of 20% of efficient cyhalothrin or 40% of chlorpyrifos by mass.
2. A method for trapping and killing Beatles, which is characterized in that the trapping and killing composition for controlling Beatles as claimed in claim 1 is placed in a trapping and killing bottle, and is placed near a bee nest to trap and kill the Beatles, wherein the trapping and killing bottle is provided with a plurality of holes for the Beatles to enter on the wall of the bottle.
3. The method of claim 2 wherein the trap bottle is an empty polypropylene plastic bottle having a plurality of holes for the bee hive beetles to enter.
4. The method as claimed in claim 2 or 3, wherein the size of the holes on the trap bottle is elliptical holes with a major axis of 3.5mm and a minor axis of 2.0 mm.
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| CN201910191069.XA CN109907075B (en) | 2019-03-12 | 2019-03-12 | Trapping and killing composition for preventing and controlling small beetles in beehives and application method |
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| CN201910191069.XA CN109907075B (en) | 2019-03-12 | 2019-03-12 | Trapping and killing composition for preventing and controlling small beetles in beehives and application method |
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| CN109907075A CN109907075A (en) | 2019-06-21 |
| CN109907075B true CN109907075B (en) | 2021-05-04 |
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| CN115669656B (en) * | 2022-10-26 | 2024-04-05 | 广东省科学院动物研究所 | Application of ocimene in preparation of small honeycomb beetles attractant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8066988B1 (en) * | 2004-12-29 | 2011-11-29 | The United States Of America As Represented By The Secretary Of Agriculture | In-hive trap and lure for control of the small hive beetle, Aethina tumida |
| CN109123248A (en) * | 2018-09-28 | 2019-01-04 | 广东省生物资源应用研究所 | A kind of man-made feeds of the small beetle of beehive |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8066988B1 (en) * | 2004-12-29 | 2011-11-29 | The United States Of America As Represented By The Secretary Of Agriculture | In-hive trap and lure for control of the small hive beetle, Aethina tumida |
| CN109123248A (en) * | 2018-09-28 | 2019-01-04 | 广东省生物资源应用研究所 | A kind of man-made feeds of the small beetle of beehive |
Non-Patent Citations (3)
| Title |
|---|
| 第二十届美国蜜蜂研究会议论文选(二);丁桂玲等;《中国蜂业》;20071201(第12期);第44-45页 * |
| 蜂巢小甲虫的危害和防治;黄文诚;《畜牧兽医科技信息》;20010131(第01期);第9页 * |
| 蜂箱小甲虫的生物学特征、入侵危害及其防控对策;赵红霞 等;《蜜蜂杂志》;20190131(第01期);第8-10页 * |
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Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Applicant before: Guangdong Institute of Applied Biological Resources |
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Inventor after: Zhao Hongxia Inventor after: Liu Fang Inventor after: Zhai Xin Inventor before: Zhao Hongxia |