CN101810201A

CN101810201A - Biological preparation for preventing and treating nest insects

Info

Publication number: CN101810201A
Application number: CN 201010148230
Authority: CN
Inventors: 周婷; 王强; 代平礼; 吴艳艳
Original assignee: Institute of Apicultural Research of Chinese Academy of Agricultural Sciences
Current assignee: Institute of Apicultural Research of Chinese Academy of Agricultural Sciences
Priority date: 2010-04-16
Filing date: 2010-04-16
Publication date: 2010-08-25

Abstract

The invention relates to a biological preparation for preventing and treating nest insects, which is prepared from the following ingredients in percentage by weight: 5 percent to 30 percent of thuringiensis, 20 to 40 percent of bait and 30 to 75 percent of auxiliary materials. The biological preparation can be used for trapping and killing nest insects in bee colonies through being spread at the bottom of a bee case, in seams of the inner wall of a case body and egg laying parts of nest insect female moths. The biological preparation can also be prepared into aqueous suspension to be sprayed on the honeycombs, and the honeycombs are stored after being dried, so the nest insects in the honeycombs can be poisoned and killed. The invention can trap, kill and poison the nest insect, has no toxic or harmful effect on bees and human bodies, and has no pollution on bee products.

Description

A kind of biologic product of preventing and treating the nest worm

Technical field

The present invention relates to a kind of biologic product of preventing and treating the nest worm, and prevent and treat the method safety and environmental protection, pollution-free to bee product.

Background technology

The nest worm is one of most important harmful animal of honeybee, and only the harm that causes every year in the U.S. is just far above 5,000,000 dollars, and China is world bee-keeping big country, is not difficult thus to calculate that the nest worm is huger to the loss meeting that China's apiculture causes.The unique distinction of nest worm is can the metabolism beeswax, and therefore, they colonize in the bee colony of field or raising specially, and rarely found at other field.The nest worm is food with the honeycomb, and moth erosion honeycomb bores the moth tunnel in honeycomb, weave silk and cocoon, and the fertility of harm bee colony, and cause bee larva death, cause the cluster honeybee to abandon nest when serious and fly away, thereby apiculture is caused irremediable loss.They all have harm to apis cerana and apis mellifera, especially to the former harm greater than the latter.The honeybee in the harm honeycomb, the honeycomb that the nest worm also causes storing suffers destructive destruction, and most bee-keepers concentrate the honeycomb of sky and preserve every year, give over to standby or the next year use, this will attract the female moth of nest worm to lay eggs herein, and the nest worm of hatching and then dependence honeycomb are bred in a large number; Usually in 2-4 month, they will make the honeycomb of storage because of almost all discarded by the moth erosion, this shows that its harm is big.When bee colony is injured gently, can manually stab dead nest worm; And when serious, the bee-keeper adopts chemicals to prevent and treat more, perhaps utilizes physical method, as freezing or water seaoning.Obviously, chemical method is easily polluted bee product, though the physical control method is harmless, but waste time and energy or need expensive equipment, and as thuringiensis (the Bacillus thuringiensis of biologic product, be called for short Bt) enjoy people's high praise, Bt contains killing gene, is the patent of WO/2009/088737 as publication number.But the Bt preparation overwhelming majority is applied in the agricultural chemicals aspect, and the controlling object that is primarily aimed at is crop pests, and wherein containing can cause a disease even carcinogenic chemical addition agent or compound medicine more, is the patent of WO/2008/092759 as publication number.

Summary of the invention

In order to adapt to the needs of bee colony control nest worm, avoid polluting bee product simultaneously, the invention provides the biologic product of a kind of biological control nest worm, and utilize trapping method and poisoning method to prevent and treat.

The technical solution adopted for the present invention to solve the technical problems is: a kind of biologic product of preventing and treating the nest worm is provided, is made up of thuringiensis, bait and auxiliary material, percentage by weight is respectively 5%-30%, 20-40% and 30-75%.Wherein the thuringiensis toxicity evaluation is 200-16000IU/mg; Bait is selected from old honeycomb, wax bits, milk powder, bean powder and dusty yeast; Auxiliary material is selected from flour, oat meal, corn flour, soluble starch and wheat bran.Utilize trapping method and poisoning method to prevent and treat, wherein the former is put in the slit of bottom board, cabinet wall this biologic product and the female moth egg of nest worm place; The latter is formulated as aqueous suspensions with this biologic product, then it is sprayed on the honeycomb, guarantees that lair wall and lair bottom all can touch medicament, honeycomb is positioned over the shady and cool ventilation place again, treats to preserve after its drying; Before using these honeycombs, utilize clear water washing honeycomb, after removing moisture, uses by honey extractor again.

The invention has the beneficial effects as follows, utilize biologic product control nest worm can reduce the use of chemicals, avoid polluting the infringement that honeybee and bee-keeping personnel are subjected to chemicals; And with the food of nest worm hobby as bait, lure that the nest worm together takes in the insect killing substance thuringiensis during bait on the feed into, thereby reach the effect of trapping nest worm.In addition, the trapping method only needs to be sprinkled into medicine in beehive and gets final product, and therefore easy to operation, chemistry and physical method are time saving and energy saving.Because the female moth hobby of nest worm is laid eggs with the place, slit of beehive inwall in the wax bits of bottom board, therefore be sprinkled into medicine in these positions, behind the nest worm feed medicine of hatching, just caused death period, and the position of spreading pesticides all directly do not contact bee product, and then avoid polluting bee product in low age.Utilize the present invention not only can trap and kill nest worm in the bee colony, and can utilize the poisoning method that idle standby honeycomb is protected.The honeycomb that the nest worm easily concentrate to destroy stores utilizes the present invention's spraying medicine in advance before preserving honeycomb, causes the nest worm just dead behind the honeycomb of minute quantity on the feed, ruins thereby avoid a large amount of honeycombs of storing to be damaged by worms by nest; Before the honeycomb that uses these spraying medicines, can utilize the clear water washing, remove moisture and residual preparation through honey extractor again; Therefore method of the present invention means of prevention more in the past safety and environmental protection has more especially been avoided preparation residual in bee product, prevents left drug harm honeybee and threatens health.

Embodiment

Embodiment 1:

Bt is carried out liquid state fermentation, and centrifugal collection active ingredient obtains dry powder after the freeze drying, and its toxicity evaluation is 8000IU/mg.Thuringiensis dry powder, old honeycomb, bean powder, soluble starch and wheat bran are mixed the back by Different Weight pulverize, concrete proportioning sees Table 1.

Table 1 is respectively organized the proportioning situation of biologic product

Embodiment 2:

The 5g preparation of getting the different tests group among the embodiment 1 respectively is sprinkled at the bottom of the beehive case and place, cabinet wall slit, not adding the bait of Bt and the mixture of auxiliary material organizes in contrast, every group is inserted 20 2-3 age in days nest worms, and 3 groups of repetitions are set, and calculates the lethality of nest worm after 6 days; Learn that by statistics nest worm lethality is 90%～100%.Every group preparation consumption can suitably be adjusted according to worm size in age and insect density.

Embodiment 3:

Experiment bee colony related among the embodiment 2 is carried out Bacteria Detection, collect wherein honey and melissa powder respectively, utilize aqua sterilisa to carry out 10 times of gradient dilutions, draw 100 μ L dilutions respectively and coat solid nutrient medium, 30 ℃, overnight incubation, the result is not separated to Bt.

Embodiment 4:

Utilize clear water that the preparation of the different tests group equal mass among the embodiment 1 is diluted 100 times, be made into aqueous suspensions, every empty honeycomb sprays the 35mL soup, is positioned over the ventilating and cooling place drying.

Embodiment 5:

In embodiment 4, insert 2-3 age in days nest worm on the dried honeycomb, 20 every group, 3 groups of repetitions are set; Control group then sprays the bait that does not add Bt and the auxiliary material mixture aqueous suspensions of equal volume; After 6 days, calculate the lethality of nest worm; The lethality of learning the nest worm by statistics is 95%～100%.The preparation consumption of every honeycomb can suitably be adjusted according to the utensil characteristics of storing honeycomb, if the utensil good leak tightness can reduce medication, otherwise, the drug concentration of increase aqueous suspensions.

Embodiment 6:

Honeycomb related among the embodiment 5 is utilized clear water washing 2 times, after drying behind the removal moisture it is put back to bee colony, after 3 days, honey in the honeycomb and melissa powder are carried out Bacteria Detection, the method for cultivation of bacteria by among the embodiment 3 is not separated to Bt.

Claims

1. biologic product of preventing and treating the nest worm, it is characterized in that: biologic product is made up of thuringiensis, bait and auxiliary material, and percentage by weight is respectively 5%-30%, 20-40% and 30-75%.

2. biologic product according to claim 1 is characterized in that: bait is selected from old honeycomb, wax bits, milk powder, bean powder and dusty yeast.

3. biologic product according to claim 1 is characterized in that: auxiliary material is selected from flour, oat meal, corn flour, soluble starch and wheat bran.

4. the trapping method of control nest worm is characterized in that: the described biologic product of claim 1 is put in the slit of bottom board, cabinet wall and the place of laying eggs of the female moth of nest worm.

5. prevent and treat the poisoning method of nest worm, it is characterized in that: the described biologic product of claim 1 is formulated as aqueous suspensions, then it is sprayed on the honeycomb, guarantee that lair wall and lair bottom all can touch medicament, again honeycomb is positioned over the shady and cool ventilation place, treats to preserve after its drying; Before using these honeycombs, utilize clear water washing honeycomb, after removing moisture, uses by honey extractor again.