CN113661982B - Composition for preventing and treating mulberry borer and application thereof - Google Patents
Composition for preventing and treating mulberry borer and application thereof Download PDFInfo
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- CN113661982B CN113661982B CN202110859653.5A CN202110859653A CN113661982B CN 113661982 B CN113661982 B CN 113661982B CN 202110859653 A CN202110859653 A CN 202110859653A CN 113661982 B CN113661982 B CN 113661982B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Agronomy & Crop Science (AREA)
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Abstract
The invention relates to a composition for preventing and treating mulberry borers and application thereof, wherein the composition for preventing and treating the mulberry borers comprises a tropism trapping substance and an isotropic trapping substance according to a mass ratio of 3:5, and the active ingredients of the tropism trapping substance are as follows: trans-3-Hexen-1-ol (E3-Hexen-1-ol), n-butyl acrylate (2-Propenoic acid, butyl ester) and n-Hexacosane (Hexacosane); the active ingredients of the isotropic induced substance are (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadecen-1-ol) and cis-11-Hexadecenal (Z11-Hexadecenal). The composition for preventing and treating the mulberry borers disclosed by the invention can be used for synergistically trapping the mulberry borer imagoes by utilizing the tropism and the disorientation of insect olfactory organs to the substances which like odors, so that the number of the mulberry borer imagoes, the mating success rate of the female and male mulberry borers and the insect population density of the secondary mulberry borers can be remarkably reduced, and the purpose of preventing and treating the mulberry borers is achieved. The experimental investigation finds that the method has high specificity for trapping and killing the target pest mulberry borer imagoes, no beneficial insects are trapped and killed, the manufacturing method is simple, the economic benefit and the ecological benefit are obvious, and the method has wide application prospects.
Description
Technical Field
A composition for preventing and controlling mulberry borer and application thereof, belonging to the technical field of pollution-free biological prevention and control of pests.
Background
The mulberry stem borer (Diaphania pylalis (Walker)) belongs to the family of Lepidoptera borer, also called as oil worm, budworm, leaf roller and the like, and occurs in other provincial silkworm areas in China except Xinjiang. The host plant of the mulberry borer is mainly mulberry and is one of the important pests of the mulberry. In recent years, the occurrence of the Jiangxi mulberry borer is generally serious and gradually becomes a superior pest in mulberry field. The mulberry borer is harmed by larvae, the larvae which are hatched at first age eat young tips and buds of the mulberry, the larvae of 3 th age begin to spit and sew into curled leaves or overlap two or more leaves, the mesophyll is eaten inside, the upper epidermis is left, and the harmed leaves often form transparent gray films. The excrement of the mulberry borer pollutes the mulberry leaves, influences the leaf quality and silkworm feeding, and is easy to cause silkworm diseases. The mulberry borer in Jiangxi area can grow for 5-6 generations in 1 year, mature larvae grow thin cocoons at cracks, wormholes and other places of trunks to live through the winter, the larvae start to move in the first ten days of 4 months every year and stop moving in the last 10 months, obvious generation overlapping phenomenon is achieved, the optimal prevention and control period of the mulberry borer larvae is 1-2 years, the prevention and control difficulty is high after the mulberry borers miss the proper prevention and control period and roll leaves, outbreak and disaster are easy to happen, the production safety of silkworm eggs is seriously influenced, the quality safety of silkworm cocoons is threatened, and even the silkworm farmers do not accept grains. According to survey statistics, the damage rate of the mulberry borers to the mulberry trees can reach 67 percent, even 100 percent; in addition, the silkworm diseases caused by the damage of the mulberry borer can be pandemic, so that the elimination rate of silkworm seeds reaches more than 12.4 percent, and the disease rate of the silkworm seeds exceeds 80 percent. Therefore, great attention must be paid to the effective prevention and control of mulberry stem borer.
At present, the prevention and treatment of mulberry stem borers mainly depend on traditional chemical pesticides, and the prevention and treatment technology is single. On one hand, chemical pesticides are excessively depended on to control the mulberry borers, so that the drug resistance of the mulberry borers is continuously improved, the control effect is gradually reduced, the control difficulty is gradually increased, meanwhile, silkworms are sensitive to chemical agents, and the mulberry leaf pesticide residue seriously influences the safe production of the mulberry silkworms; on the other hand, if chemical pesticides are used for a long time, the mulberry field has serious environmental pollution, which not only harms the health of people and livestock, but also threatens the living environment of the natural enemy organisms of the pests in the mulberry field, so that the quantity of the natural enemies of the pests is reduced rapidly, and the ecological natural regulation and control of the mulberry field are damaged.
Most of the researches on the tropism and disorientation of insect olfactory organ to favorite odorant are insect food attractant and sex attractant. The food attractant and the sex attractant are important technical measures for developing green prevention and control of crop pests and promoting reduction and pest control of pesticides. In recent years, the research and application of pest control technology for controlling pests by using food or sex attractant are widely carried out, compared with the traditional chemical pesticide, the food or sex attractant depends on the omnibearing three-dimensional coverage of special odor substances in the air, has strong specificity for trapping and killing pests, high efficiency and no pollution, and solves the defect of untimely chemical control. However, no report is found in the technical research of utilizing the tropism substance and the disorientation substance to cooperatively control the mulberry borer imagoes.
Disclosure of Invention
Aiming at the problems in the prior art of preventing and controlling the mulberry borer imagoes, the invention aims to prevent and control the mulberry borer imagoes through the cooperation of a tropism trapping substance and an isotropic trapping substance. The invention is realized by adopting the following technical scheme.
The invention provides a composition for preventing and treating mulberry borers and application thereof, wherein the composition consists of a tropism trapping substance and an isotropic trapping substance according to a mass ratio of 3: 5; the active ingredients of the tropism trapping substance comprise trans-3-Hexen-1-ol (E3-Hexen-1-ol), n-butyl acrylate (2-Propenoic acid, butyl ester) and n-Hexacosane (Hexacosane) according to a mass ratio of 7:5: 3; the active ingredients of the isotropic attraction substance comprise (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadienen-1-ol) and cis-11-Hexadecenal (Z11-Hexadecenal) according to the mass ratio of 1: 1.
The composition is applied to controlling mulberry borers in mulberry fields.
A composition for preventing and treating mulberry borer is prepared by dissolving the composition in redistilled n-hexane to prepare a to-be-tested agent, and dripping the to-be-tested agent onto a slow-release carrier in the eclosion period of the mulberry borer to test the intercarling trapping effect.
Furthermore, the slow release carrier is any one of filter paper, a cotton wool ball, a rubber plug and paraffin oil.
The beneficial effects of the invention are as follows: the mulberry borer attractant has special alcohols, esters, alkanes and aldehydes, has attraction and disorientation interference effects on mulberry borer adults, can break through the influence of limiting factors such as the leaf rolling damage habit of the mulberry borer larvae, obvious generation overlapping and the like on the prevention and treatment effect, effectively reduces the number of the mulberry borer adults and the larva base number of the secondary mulberry borers, reduces the damage of the mulberry borers to mulberry trees, effectively reduces the use times and the use amount of pesticides, and reduces or avoids environmental pollution. The novel attractant has the advantages of strong specificity, high efficiency, environmental friendliness, safety to people and livestock and the like, can be used in the field of comprehensive prevention and control of mulberry borers in mulberry fields, is simple in preparation method, remarkable in economic benefit and ecological benefit, and has wide application prospects.
Drawings
FIG. 1 is a statistical chart of the trapping quantity of different formula compositions on the mulberry borer imagoes.
Detailed Description
The invention is further illustrated by the following examples which are intended to be purely exemplary of the invention and are not intended to limit its scope in any way. The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art; furthermore, modifications of the invention shall be apparent to those skilled in the art and all changes which fall within the spirit of the invention and the scope of the appended claims are intended to be embraced thereby.
At the beginning of an experiment, the formulations of the embodiments 1 to 6 of the invention are respectively dissolved by 50ml of heavy-distilled n-hexane to prepare a to-be-tested agent, 1ml of the to-be-tested agent is dripped into each slow release carrier in the eclosion period of the mulberry borers to carry out an intercarvating effect test, and each slow release carrier contains. The slow release carriers are placed in the central position of the trap, and the same amount of reagent to be tested is added according to the volatilization condition of the reagent contained in each slow release carrier, so that the reagent is not completely volatilized during the test.
Example 1
Pure tropism trapping material formula 1: 14mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 10mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 6mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping substance formula 1 (expressed as CQ 1).
Pure tropism trapping material formula 2: 21mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 15mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 9mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping material formula 2 (expressed as CQ 2).
Pure tropism trapping material formula 3: 28mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 20mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 12mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping material formula 3 (expressed as CQ 3).
Example 2
The pure isotropic trapping substance formula comprises the following components: (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) 37.5mg and 11Z-Hexadecenal (Z11-Hexadecenal)37.5mg were mixed uniformly to obtain the mutagenic substance formulation 1 (expressed as CM).
Example 3
The formula 1 of the composition for preventing and treating mulberry borer: the pure tropism trapping material and the pure isotropic trapping material are composed according to the mass ratio of 2:5, and comprise 14mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 10mg of n-butyl acrylate (2-Propenoic acid, butyl ester) and 6mg of n-Hexacosane (Hexacosane); (cis 3, trans 16) -5-methyloctadecadienol (E2, Z15, Me 14-octadienen-1-ol) 37.5mg and 11Z-Hexadecenal (Z11-Hexadecenal)37.5mg, and the components are uniformly mixed to obtain the composition formula 1 (represented by ZH 1) for preventing and treating the mulberry borer.
Respectively placing the slow-release carrier dropwise added with the pure CQ1, CM and ZH1 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. The traps containing 4 treatments of the test agents were arranged symmetrically in a square with equal spacing, with consistent suspension height and suspension time, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: compared with the blank control which does not trap the mulberry borer imagoes, the CQ1 trap is treated to trap 198.4 heads of the mulberry borer imagoes; treating the CM trapper to trap 271.3 heads of mulberry borer imagoes; when ZH1 traps were treated to trap adult mulberry borer 472.8, the trapping amount of the combination formula 1 was increased by 0.66% compared with the sum of the traps alone.
Example 4
The mulberry borer preventing and treating composition formula 2: the pure tropism decoy substance and the pure tropism decoy substance are composed according to the mass ratio of 3:5, and comprise 21mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 15mg of n-butyl acrylate (2-Propenoic acid, butyl ester) and 9mg of n-Hexacosane (Hexacosane); (cis 3, trans 16) -5-methyloctadecadienol (E2, Z15, Me 14-octadienen-1-ol) 37.5mg and 11Z-Hexadecenal (Z11-Hexadecenal)37.5mg, and the components are uniformly mixed to obtain the composition formula 1 (represented by ZH 2) for preventing and treating the mulberry borer.
Respectively placing the slow-release carrier dropwise added with the pure CQ2, CM and ZH2 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. Traps treated with 4 test agents were arranged symmetrically in a square with equal spacing, hanging height and hanging time were kept consistent, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: treating CQ2 trap to trap 234.1 head of mulberry borer; by treating the ZH2 trap to trap 573.6 heads of the mulberry borer imagoes, the trapping amount of the combined formula 2 is improved by 13.49 percent compared with the sum of the traps alone.
Example 5
Formula 3 of the composition for preventing and treating mulberry borers: the pure tropism decoy substance and the pure tropism decoy substance are composed according to a mass ratio of 4:5, and comprise 28mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 20mg of n-butyl acrylate (2-Propenoic acid, butyl ester) and 12mg of n-Hexacosane (Hexacosane); (cis 3, trans 16) -5-methyloctadecadienol (E2, Z15, Me 14-octadienen-1-ol) 37.5mg and 11Z-Hexadecenal (Z11-Hexadecenal)37.5mg, and the components are uniformly mixed to obtain the composition formula 1 (represented by ZH 3) for preventing and treating the mulberry borer.
Respectively placing the slow-release carrier dropwise added with the pure CQ1, CM and ZH1 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. The traps containing 4 treatments of the test agents were arranged symmetrically in a square with equal spacing, with consistent suspension height and suspension time, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: treating CQ3 trap to trap 236.5 head of mulberry borer imagoes; when the ZH3 trap is treated to trap 517.4 heads of the mulberry borer imagoes, the trapping amount of the combined formula 2 is increased by 1.89% compared with the sum of the traps alone.
The trapping effect of the compositions with different formulas on the mulberry borers is shown as follows: the trapping effect of the composition of the tropism trapping substance and the disorientation trapping substance is better than the sum of the effects of single use, wherein the composition for preventing and treating the mulberry borers, which is composed of the pure tropism trapping substance and the pure disorientation trapping substance according to the mass ratio of 3:5, has the best formula effect, the efficiency is improved by 13.49%, and the prevention effect is remarkably different from the prevention effect of the composition for preventing and treating the mulberry borers according to the mass ratios of 2:5 and 4: 5. Therefore, the formula provided by the invention has an obvious trapping effect on the mulberry borers, and can be applied to mulberry gardens for carrying out pollution-free control on the mulberry borers.
Claims (4)
1. The composition for preventing and treating the mulberry borers is characterized by comprising a tropism trapping substance and a disorientation trapping substance according to a mass ratio of 3: 5; the active ingredients of the tropism trapping material comprise trans-3-hexene-1-alcohol, n-butyl acrylate and n-hexacosane according to the mass ratio of 7:5: 3; the active ingredients of the disorientation induced-collection substance comprise (cis-3, trans-16) -5-methyloctadecadienol and 11Z-hexadecenal according to the mass ratio of 1: 1.
2. The composition of claim 1, wherein the composition is used for controlling mulberry borers in mulberry fields.
3. A composition for controlling mulberry borers, which is characterized in that the composition of claim 1 is dissolved in heavy-distilled n-hexane to prepare a test agent, and the test agent is dripped on a slow-release carrier to carry out trapping effect test.
4. The composition for controlling mulberry borer according to claim 3, wherein the slow release carrier is any one of filter paper, absorbent cotton balls, rubber plugs and paraffin oil.
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| CN111096323A (en) * | 2019-12-27 | 2020-05-05 | 四川省金桑庄园农业发展股份有限公司 | Mulberry borer attractant and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111096323A (en) * | 2019-12-27 | 2020-05-05 | 四川省金桑庄园农业发展股份有限公司 | Mulberry borer attractant and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李玉林 等.自然条件下桑螟种群的性比统计及雌性性腺提取物的气相色谱-质谱分析.《蚕业科学》.2018,第44卷(第1期),第16-24页. * |
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