CN113661982A - Composition for preventing and treating mulberry borers and application thereof - Google Patents
Composition for preventing and treating mulberry borers and application thereof Download PDFInfo
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- CN113661982A CN113661982A CN202110859653.5A CN202110859653A CN113661982A CN 113661982 A CN113661982 A CN 113661982A CN 202110859653 A CN202110859653 A CN 202110859653A CN 113661982 A CN113661982 A CN 113661982A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The composition for preventing and treating the muller moth comprises a tropism trapping substance and a disorientation trapping substance according to a mass ratio of 2-4: 5. The tropism trapping material comprises the following effective components: trans-3-Hexen-1-ol (E3-Hexen-1-ol), n-butyl acrylate (2-Propenoic acid, butyl ester), n-Hexacosane (Hexacosane); the active ingredients of the disorientation trapping substance are (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) and cis-11-Hexadecenal (Z11-Hexadecenal). The composition for preventing and treating the mulberry borers provided by the invention synergistically traps and collects the mulberry borer imagoes by utilizing the tropism and the disorientation of insect olfactory organs to favorite odor substances, can obviously reduce the number of the mulberry borer imagoes, the mating success rate of the mulberry borer male and female insects and the insect population density of the mulberry borers of the next generation, and achieves the purpose of preventing and treating the mulberry borers. The experimental investigation finds that the method has high trapping and killing specificity on the target pest mulberry borer imagoes, beneficial insects are not trapped and killed, the manufacturing method is simple, the economic benefit and the ecological benefit are obvious, and the method has wide application prospect.
Description
Technical Field
A composition for preventing and controlling mulberry borers and application thereof, belonging to the technical field of pollution-free biological prevention and control of pests.
Background
The mulberry stem borer (Diaphania pyloiis (Walker)) belongs to the family of Lepidoptera (Bombycidae), also called oil worm, budworm, leaf roller, etc., and occurs in other provinces of silkworm in China except Xinjiang. The host plant of the mulberry borer is mainly mulberry and is one of the important pests of the mulberry. In recent years, the occurrence of Jiangxi mulberry borer is generally serious, and the Jiangxi mulberry borer has gradually become a dominant pest in mulberry gardens. The mulberry borer is harmed by larvae, the larvae which are hatched at first age eat young tips and buds of the mulberry, the larvae of 3 th age begin to spit and sew into curled leaves or overlap two or more leaves, the mesophyll is eaten inside, the upper epidermis is left, and the harmed leaves often form transparent gray films. The excrement of the mulberry borer pollutes the mulberry leaves, influences the leaf quality and silkworm feeding, and is easy to cause silkworm diseases. The mulberry borer in Jiangxi area can grow for 5-6 generations in 1 year, mature larvae grow thin cocoons at cracks, wormholes and other places of trunks to live through the winter, the larvae start to move in the first ten days of 4 months every year and stop moving in the last 10 months, obvious generation overlapping phenomenon is achieved, the optimal prevention and control period of the mulberry borer larvae is 1-2 years, the prevention and control difficulty is high after the mulberry borers miss the proper prevention and control period and roll leaves, outbreak and disaster are easy to happen, the production safety of silkworm eggs is seriously influenced, the quality safety of silkworm cocoons is threatened, and even the silkworm farmers do not accept grains. According to survey statistics, the damage rate of the mulberry borers to the mulberry trees can reach 67 percent, even 100 percent; in addition, the silkworm diseases caused by the damage of the mulberry borer can be pandemic, so that the elimination rate of silkworm seeds reaches more than 12.4 percent, and the disease rate of the silkworm seeds exceeds 80 percent. Therefore, great attention must be paid to the effective prevention and control of mulberry stem borer.
At present, the prevention and treatment of the mulberry stem borer mainly depends on the traditional chemical pesticide, and the prevention and treatment technology is single. On one hand, the chemical pesticide is excessively depended on to control the mulberry borers, so that the drug resistance of the mulberry borers is continuously improved, the control effect is gradually reduced, the control difficulty is gradually increased, meanwhile, the silkworms are sensitive to the chemical agent, and the mulberry leaf pesticide residue seriously influences the safe production of the mulberry silkworms; on the other hand, the chemical pesticide is used for a long time, the environment pollution of the mulberry field is serious, the health of people and livestock is harmed, the living environment of the natural enemy organisms of the pests in the mulberry field is threatened, the number of the natural enemies of the pests is reduced, and the ecological natural regulation and control of the mulberry field are damaged.
Most of the researches on the tropism and disorientation of insect olfactory organ to favorite odorant are insect food attractant and sex attractant. The food attractant and the sex attractant are important technical measures for developing green prevention and control of crop pests and promoting reduction and pest control of pesticides. In recent years, the research and application of pest control technology for controlling pests by using food or sex attractant are widely carried out, compared with the traditional chemical pesticide, the food or sex attractant depends on the omnibearing three-dimensional coverage of special odor substances in the air, has strong specificity for trapping and killing pests, high efficiency and no pollution, and solves the defect of untimely chemical control. However, no report is found in the technical research of utilizing the tropism substance and the disorientation substance to cooperatively control the mulberry borer imagoes.
Disclosure of Invention
Aiming at the problems in the prior art of controlling the mulberry borer imagoes, the invention aims to cooperatively control the mulberry borer imagoes through a tropism trapping substance and a disorientation trapping substance. The invention is realized by adopting the following technical scheme.
The invention provides a composition for preventing and treating mulberry borers and application thereof, wherein the composition comprises a tropism trapping substance and a disorientation trapping substance according to a mass ratio of 2-4: 5; the tropism trapping material comprises the following effective components: trans-3-Hexen-1-ol (E3-Hexen-1-ol), n-butyl acrylate (2-Propenoic acid, butyl ester), n-Hexacosane (Hexacosane); the active ingredients of the disorientation trapping substance are (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) and cis-11-Hexadecenal (Z11-Hexadecenal).
On the basis of the scheme, the active ingredients of the tropism trapping substance comprise trans-3-Hexen-1-ol (E3-Hexen-1-ol), n-butyl acrylate (2-Propeneic acid, butyl ester) and n-Hexacosane (Hexacosane) according to the mass ratio of 7:5: 3.
On the basis of the scheme, the active ingredients of the disorientation trapping substance comprise (cis 3, trans 16) -5-methyloctadecadienol (E2, Z15, Me 14-octadienen-1-ol) and cis-11-Hexadecenal (Z11-Hexadecenal) according to the mass ratio of 1: 1.
On the basis of the scheme, the mass ratio of the tropism trapping substance to the disorientation trapping substance is 3: 5.
The composition is applied to preventing and treating mulberry borers in mulberry fields.
A composition for preventing and treating mulberry borer and application thereof are disclosed, wherein the composition is dissolved in heavy-distilled n-hexane to prepare a to-be-tested agent, and the to-be-tested agent is dripped onto a slow-release carrier during eclosion period of the mulberry borer to perform intercropping effect test.
The slow release reconditioning can be any one of filter paper, absorbent cotton ball, rubber plug, and paraffin oil.
The invention has the beneficial effects that: the mulberry borer attractant has special alcohols, esters, alkanes and aldehydes, has attraction and disorientation interference effects on mulberry borer adults, can break through the influence of limiting factors such as the leaf rolling damage habit of the mulberry borer larvae, obvious generation overlapping and the like on the prevention and treatment effect, effectively reduces the number of the mulberry borer adults and the larva base number of the secondary mulberry borers, reduces the damage of the mulberry borers to mulberry trees, effectively reduces the use times and the use amount of pesticides, and reduces or avoids environmental pollution. The novel attractant has the advantages of strong specificity, high efficiency, environmental friendliness, safety for people and livestock and the like, can be used in the field of comprehensive prevention and control of mulberry borers in mulberry gardens, is simple in preparation method, remarkable in economic benefit and ecological benefit and has wide application prospect.
Drawings
FIG. 1 is a statistical chart of the trapping quantity of mulberry borer imagoes by different formula compositions.
Detailed Description
The present invention is further illustrated by the following specific examples, which are intended to be purely exemplary of the invention and are not intended to limit its scope in any way. The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art; further, modifications to the invention, as would be obvious to those of ordinary skill in the art, are intended to be included within the scope of the present invention as defined by the following claims and their equivalents.
At the beginning of an experiment, the formulations of the embodiments 1 to 6 of the invention are respectively dissolved by 50ml of heavy-distilled n-hexane to prepare a to-be-tested agent, 1ml of the to-be-tested agent is dripped into each slow release carrier in the eclosion period of the mulberry borers to carry out an intercarvating effect test, and each slow release carrier contains. The slow release carriers are placed in the central position of the trap, and the same amount of reagent to be tested is added according to the volatilization condition of the reagent contained in each slow release carrier, so that the reagent is not completely volatilized during the test.
Example 1
Pure tropism trapping material formula 1: 14mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 10mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 6mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping substance formula 1 (expressed as CQ 1).
Pure tropism trapping material formula 2: 21mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 15mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 9mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping material formula 2 (expressed as CQ 2).
Pure tropism trapping material formula 3: 28mg of trans-3-Hexen-1-ol (E3-Hexen-1-ol), 20mg of n-butyl acrylate (2-Propeneic acid, butyl ester) and 12mg of n-Hexacosane (Hexacosane) were mixed uniformly to obtain pure tropism trapping substance formula 3 (expressed as CQ 3).
Example 2
The pure disorientation induced-collection substance formula comprises the following components: (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) 37.5mg, 11Z-Hexadecenal (Z11-Hexadecenal)37.5mg, and the mixture was mixed uniformly to obtain the mutagenic substance formulation 1 (indicated as CM).
Example 3
The formula of the composition for preventing and treating mulberry borers 1: the pure tropism trapping substance and the pure isotropic trapping substance are composed according to a mass ratio of 2:5, and comprise 14mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 10mg of n-butyl acrylate (2-Propenoic acid, butyl ester), 6mg of n-Hexacosane (Hexacosane), 37.5mg of (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) and 37.5mg of 11Z-Hexadecenal (Z11-Hexadecenal), and a composition formula 1 (represented by ZH 1) for preventing and controlling the mulberry borer is obtained after uniform mixing.
Respectively placing the slow-release carrier dropwise added with the pure CQ1, CM and ZH1 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. The traps containing 4 treatments of the test agents were arranged symmetrically in a square with equal spacing, with consistent suspension height and suspension time, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: compared with the blank control which does not trap the mulberry borer imagoes, the CQ1 trap is treated to trap 198.4 heads of the mulberry borer imagoes; treating the CM trapper to trap 271.3 heads of mulberry borer imagoes; when ZH1 traps were treated to trap adult mulberry borer 472.8, the trapping amount of the combination formula 1 was increased by 0.66% compared with the sum of the traps alone.
Example 4
Formula 2 of the composition for preventing and treating mulberry borers: the pure tropism trapping substance and the pure isotropic trapping substance are composed according to the mass ratio of 3:5, and comprise 21mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 15mg of n-butyl acrylate (2-Propenoic acid, butyl ester), 9mg of n-Hexacosane (Hexacosane), 37.5mg of (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) and 37.5mg of 11Z-Hexadecenal (Z11-Hexadecenal), and a composition formula 1 (represented by ZH 2) for preventing and controlling the mulberry borer is obtained after uniform mixing.
Respectively placing the slow-release carrier dropwise added with the pure CQ2, CM and ZH2 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. The traps containing 4 treatments of the test agents were arranged symmetrically in a square with equal spacing, with consistent suspension height and suspension time, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: treating CQ2 trap to trap 234.1 head of mulberry borer; by treating the ZH2 trap to trap 573.6 heads of the mulberry borer imagoes, the trapping amount of the combined formula 2 is improved by 13.49 percent compared with the sum of the traps alone.
Example 5
Formula 3 of the composition for preventing and treating mulberry borers: the pure tropism trapping substance and the pure isotropic trapping substance are composed according to a mass ratio of 4:5, and comprise 28mg of trans-3-hexene-1-ol (E3-Hexen-1-ol), 20mg of n-butyl acrylate (2-Propenoic acid, butyl ester), 12mg of n-Hexacosane (Hexacosane), 37.5mg of (cis 3, trans 16) -5-methyl octadecadienol (E2, Z15, Me 14-octadien-1-ol) and 37.5mg of 11Z-Hexadecenal (Z11-Hexadecenal), and a formula 1 (represented by ZH 3) of the composition for preventing and controlling the mulberry borer is obtained after uniform mixing.
Respectively placing the slow-release carrier dropwise added with the pure CQ1, CM and ZH1 to-be-tested agents in a trap, and taking the same amount of redistilled n hexane as a control to test the luring effect of the paradise mulberry borer. The traps containing 4 treatments of the test agents were arranged symmetrically in a square with equal spacing, with consistent suspension height and suspension time, and 3 replicates were set. According to the prediction and forecast of the mulberry borers, the eclosion period of the mulberry borers in the 9-month period of the large outbreak of the mulberry borers is selected for the test, the continuous investigation is carried out for 10d, the treatment in the investigation period is carried out at 16:00 in the afternoon at the same time or the test agent is added, and the number of the mulberry borers trapped is recorded in the 9:00 in the morning. From the data of FIG. 1, it can be seen that: treating CQ3 trap to trap 236.5 head of mulberry borer imagoes; when the ZH3 trap is treated to trap 517.4 heads of the mulberry borer imagoes, the trapping amount of the combined formula 2 is increased by 1.89% compared with the sum of the traps alone.
The trapping effect of the compositions with different formulas on the mulberry borers is shown as follows: the trapping effect of the composition of the tropism trapping substance and the disorientation trapping substance is better than the sum of the effects of single use, wherein the composition for preventing and treating the mulberry borers, which is composed of the pure tropism trapping substance and the pure disorientation trapping substance according to the mass ratio of 3:5, has the best formula effect, the efficiency is improved by 13.49%, and the prevention effect is remarkably different from the prevention effect of the composition for preventing and treating the mulberry borers according to the mass ratios of 2:5 and 4: 5. Therefore, the formula provided by the invention has an obvious trapping effect on the mulberry borers and can be applied to mulberry gardens for carrying out pollution-free control on the mulberry borers.
Claims (7)
1. The composition for preventing and treating the mulberry borers is characterized by comprising a tropism trapping substance and a disorientation trapping substance in a mass ratio of 2-4: 5; the tropism trapping material comprises the following effective components: trans-3-hexen-1-ol, n-butyl acrylate, n-hexacosane; the active ingredients of the disorientation trapping substance are (cis 3, trans 16) -5-methyloctadecadienol and cis-11-hexadecenal.
2. The composition for controlling multocida borers and the application of the composition are characterized in that the effective components of the tropism trapping substance comprise trans-3-hexen-1-ol, n-butyl acrylate and n-hexacosane in a mass ratio of 7:5: 3.
3. The composition for controlling mulberry borers and the application thereof according to claim 1 are characterized in that the active ingredients of the fascinating trapping substance comprise (cis 3, trans 16) -5-methyloctadecadienol and 11Z-hexadecenal in a mass ratio of 1: 1.
4. The composition for controlling mulberry borers according to any one of claims 1 to 3, wherein the mass ratio of the tropism trapping substance to the disorientation trapping substance is 3: 5.
5. The composition of any one of claims 1 to 3 is applied to controlling mulberry borers in mulberry fields.
6. The composition for preventing and treating the mulberry borers is characterized in that the composition of any one of claims 1 to 3 is dissolved in heavy-distilled n-hexane to prepare a test agent, and the test agent is dripped on a slow-release carrier to carry out trapping effect test.
7. The sustained-release carrier according to claim 6, which is any one of filter paper, a cotton wool ball, a rubber plug and paraffin oil.
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CN202110859653.5A CN113661982B (en) | 2021-07-28 | 2021-07-28 | Composition for preventing and treating mulberry borer and application thereof |
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CN202110859653.5A CN113661982B (en) | 2021-07-28 | 2021-07-28 | Composition for preventing and treating mulberry borer and application thereof |
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CN113661982B CN113661982B (en) | 2022-06-28 |
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CN111096323A (en) * | 2019-12-27 | 2020-05-05 | 四川省金桑庄园农业发展股份有限公司 | Mulberry borer attractant and application thereof |
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CN111096323A (en) * | 2019-12-27 | 2020-05-05 | 四川省金桑庄园农业发展股份有限公司 | Mulberry borer attractant and application thereof |
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李玉林 等: "自然条件下桑螟种群的性比统计及雌性性腺提取物的气相色谱-质谱分析", 《蚕业科学》, vol. 44, no. 1, 31 December 2018 (2018-12-31), pages 16 - 24 * |
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