CN109362740B

CN109362740B - Seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin and application

Info

Publication number: CN109362740B
Application number: CN201811472282.XA
Authority: CN
Inventors: 李敏; 王鹏
Original assignee: Shaanxi Jiayi Lande Biological Engineering Co ltd
Current assignee: SHAANXI JIAYI LANDE BIOLOGICAL ENGINEERING Co.,Ltd.
Priority date: 2018-12-04
Filing date: 2018-12-04
Publication date: 2020-11-10
Anticipated expiration: 2038-12-04
Also published as: CN109362740A

Abstract

The invention relates to a seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin and application thereof, wherein the effective components of the seed coating agent are chlorantraniliprole, fluopyram and lambda-cyhalothrin; the mass contents of the effective components of chlorantraniliprole, fluopyram and lambda-cyhalothrin are respectively 1-15%, 1-10% and 1-8%, and the balance is auxiliary components. The seed coating agent can be effectively used for preventing and treating grub, mole cricket, wireworm and cutworm diseases and insect pests of soil insects of peanut, potato, corn and wheat crops.

Description

Seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin and application

Technical Field

The invention relates to the technical field of pesticide compounding, in particular to a seed coating agent containing chlorantraniliprole, fluopyram and efficient cyhalothrin.

Background

The seed coating agent is a pesticide preparation with film-forming property for treating crop seeds, and can solidify a film containing pesticide effective components on the surface of seeds, namely seed coating. The seed coating agent absorbs water and swells in soil but is not dissolved, the water and air required by the normal germination of seeds are not influenced to pass through, the contained pesticide effective components can be slowly released, the seeds are protected from being damaged by underground pests and various germs, and the seed coating agent has the effects of preventing and treating diseases and pests in seedling stage, improving the germination rate of the seeds, promoting the healthy growth of crops in seedling stage and the like.

Chlorantraniliprole (chlorantraniliprole), formula: C18H14BrCl2N5O2, chemical name: 3-bromo-N- [ 4-chloro-2-methyl-6- [ (methylcarbamoyl) benzene ] -1- (3-chloropyridin-2-yl) -1H-pyrazole-5-carboxamide. The chlorantraniliprole is an o-formamidobenzamide broad-spectrum pesticide, is mainly used for preventing and controlling lepidoptera pests of various crops, and has better prevention effect on other pests. The action mechanism is to activate ryanodine receptor, release calcium stored in smooth muscle and striated muscle cells, cause muscle conditioning weakness and mahonia until the pests die. The action modes are stomach toxicity and contact toxicity, and the stomach toxicity is the main action mode. Chlorantraniliprole can permeate into plant bodies through the surfaces of stems and leaves, and can be absorbed by roots and move in xylem.

Fluopyram (fluopyram), formula: C17H20ClN3O2, chemical name: 2, 6-dichloro-N- [ (3-chloro-5-trifluoromethyl-2-pyridyl) methyl ] benzamide, a pyridylethylbenzamide fungicide, inhibits mitochondrial respiration by hindering electron transfer from dehydromyceliophthora succinogenes in the respiratory chain. Can be used for preventing and controlling various diseases such as gray mold, powdery mildew, sclerotinia and the like on more than 70 crops including grapes, pears, stone fruits, vegetables, field crops and the like.

Lambda-cyhalothrin (lambda-cyhalothrin), chemical formula: C23H19ClF3NO3, chemical name: 3- (2-chloro-3, 3, 3-trifluoropropenyl) -2, 2-dimethylcyclopropanecarboxylic acid alpha-cyano-3-phenoxybenzyl ester, namely cyhalothrin and cyhalothrin. Inhibit the conduction of the nerve axon part of the insect, has the functions of avoiding, knocking down and poisoning the insect, has wide insecticidal spectrum, higher activity and quick drug effect, and is rain-proof after being sprayed.

Grub (anomalla cuprea), larva of tortoise shell, has the most serious harm to orchard, seedlings and other crops in spring and autumn. When the grubs bite the tender stems of the seedlings, tuber tubers of the yams are drilled into holes, and when the plants are withered and yellow and die, the tuber tubers are transferred to other plants for continuous harm. In addition, wounds due to grubs can also induce disease. The feeding property of the phytophagous grubs is wide, the grubs harm various crops, economic crops and flower seedlings, and the grubs like to eat newly sown seeds, roots, tubers and seedlings, are worldwide underground pests and have great harm.

Gryllotalpa (Gryllotalpa) is a general name of insects of Gryllotalpa family of the orders Lauraceae of the Insecta of the phylum of various habitat, and lives in soil. Mole cricket is a polyphagic pest, is fond of eating various vegetables, and has serious harm to vegetable seedbeds and transplanted vegetable seedlings. The adult mole cricket and nymph bite the seeds and young buds which are just sown in the soil, or bite the roots and stems of the young seedlings to make the young seedlings wither and the damaged roots are in a messy state. The mole cricket moves underground, penetrates surface soil into a plurality of tunnels, enables the roots of the seedlings to be ventilated and the soil to be separated, causes the seedlings to be withered and killed due to dehydration, lacks seedlings and breaks ridges, seriously even destroys seeds, and greatly reduces the yield of vegetables.

The golden insect is a general name of insect larvae of Coleoptera (Coleoptera) percussopunctata (Elaterae), and the larvae live in soil for a long time and are mainly harmful to cereals, potatoes, beans, beets, cotton, various vegetables, forest seedlings and the like. The larvae can bite the seeds just sown, damage endosperm to prevent germination, and if seedlings emerge, the seedlings can be withered due to damage to the underground parts of fibrous roots, main roots and stems. The main roots are not regular, and can also enter tubers and root tubers.

The main types of cutworms include black cutworms (Agrotis ipsilon), black cutworms (Agrotistokionis), yellow cutworms (Euxoa segetum), and the like. The cutworm is characterized in that the base of the stem is bitten off to cause the seedling shortage and the strip breaking of the crops. Wherein the agrotis ypsilon is widely distributed and the quantity is more serious. The cutworms like to live in wet clay ground, and the cutworms in the size and the size are often mixed. The method is serious in low-wet depression along lakes and rivers, flooding areas, water passing areas, seasonal water accumulation areas and the like. The aged larvae overwinter in soil, the larvae dig quickly, are fiercely sexual, often kill each other, and resist hunger.

Strong and has certain tropism to fresh paulownia leaves and flowers. The adult worms are out of the night in the daytime, have strong tendency to black light lamps, have obvious tendency to sugar wine vinegar liquid and have the characteristic of seasonal long-distance migration.

At present, the single-dose product has high use frequency, so that the drug resistance of pests is gradually enhanced, the control effect on the pests is greatly reduced, and the ideal control effect cannot be achieved even if the dosage is increased. At present, no report about seed coating agents of chlorantraniliprole, fluopyram and lambda-cyhalothrin exists. On the basis of a large number of experiments, the three pesticides are found to have a synergistic effect on diseases of seeds, particularly soil insects such as grubs, mole cricket, wireworms and cutworms for controlling peanut, potato and corn wheat crops.

Disclosure of Invention

The invention aims to provide a seed coating agent containing chlorantraniliprole, fluopyram and efficient cyhalothrin, which is used for coating seeds, has good insecticidal effect and is used for preventing and treating grub, mole cricket, wireworm and cutworm diseases and insect pests of peanut, potato, corn and wheat crops.

In order to realize the purpose of the invention, the invention adopts the following technical scheme:

a seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin is characterized in that: the effective components are chlorantraniliprole, fluopyram and lambda-cyhalothrin; the mass ratio of the effective components of the chlorantraniliprole, the fluopyram and the lambda-cyhalothrin is 1-15:1-10: 1-8.

The mass contents of the effective components of chlorantraniliprole, fluopyram and lambda-cyhalothrin are respectively 1-15%, 1-10% and 1-8%, and the balance is auxiliary components.

The pesticide preparation which can be prepared from the seed coating agent is a suspension seed coating agent, seed treatment dispersible powder and seed treatment dispersible granules.

When the seed coating agent is a suspension seed coating agent, the mass ratio of the components in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 1 to 5 percent of dispersant, 1 to 5 percent of antifreeze, 0.1 to 5 percent of film forming agent, 1 to 5 percent of thickening agent, 0.1 to 2 percent of defoaming agent, 1 to 5 percent of warning color and the balance of water to 100 percent; the specific processing steps are as follows: fully mixing the components in the formula according to the formula, uniformly mixing by high-speed shearing, and sanding in a sand mill for 2-3 hours to ensure that the particle size is below 5 microns, thus obtaining the suspended seed coating agent.

When the seed coating agent is dispersible powder, the mass ratio of each component in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 2-8% of dispersing agent, 1-6% of wetting agent, 1-10% of film-forming agent, 1-5% of warning color and 100% of filler; the specific processing steps are as follows: the components in the formula are mixed according to the formula, the mixture is uniformly mixed and stirred in a mixing stirrer, the mixture is crushed by a jet mill, and the seed treatment dispersible powder is obtained after uniform mixing.

When the dosage form of the seed coating agent is dispersible granules, the weight ratio of the components in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 1-6% of dispersing agent, 2-6% of wetting agent, 1-10% of disintegrating agent, 1-5% of film-forming agent, 1-5% of warning color and 100% of filler; the specific processing steps are as follows: the components are uniformly mixed according to the formula, are subjected to air flow crushing and then are uniformly mixed, and then are added into a fluidized bed granulator for granulation, or are added with water for kneading and then are subjected to extrusion granulation, drying, screening and sampling analysis, so that the seed treatment dispersible granule is obtained.

The dispersing agent is selected from one or a mixture of two or more of lignosulfonate, naphthalene sulfonate, polycarboxylate, cyclohexanone-formaldehyde polycondensate, naphthalene sulfonate-formaldehyde polycondensate, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether-formaldehyde condensate sulfate and sorbitan polyoxyethylene ether;

the wetting agent is selected from one or a mixture of two or more of sodium dodecyl sulfate, calcium dodecyl benzene sulfonate, nekal, wetting penetrant T, tea saponin, fatty alcohol-polyoxyethylene ether, alkyl naphthalene sulfonate and the like;

the disintegrating agent is one or a mixture of two or more of bentonite, urea, ammonium sulfate, sodium sulfate and sodium carbonate;

the thickening agent is one or a mixture of two or more of xanthan gum, magnesium aluminum silicate, hydroxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, sodium alginate, Arabic gum, polyethylene glycol, polyvinylpyrrolidone, polyacrylamide, sodium polyacrylate and the like;

the film-forming agent is one or a mixture of two or more of xanthan gum, hydroxymethyl cellulose, polyvinyl alcohol, sodium alginate, polyvinyl alcohol, Arabic gum, polyacrylamide, polyethylene glycol, polyvinyl acetate, starch and the like;

the antifreezing agent is one or a mixture of two or more of ethylene glycol, propylene glycol, glycerol, urea, ammonium sulfate, sodium sulfate and sodium chloride;

the defoaming agent is selected from one or a mixture of two or more of organic silicon defoaming agents, fatty alcohol compounds and fatty acid compounds;

the filler is selected from one or a mixture of two or more of kaolin, light calcium carbonate, diatomite, bentonite, attapulgite, argil, white carbon black and corn starch;

the warning color is selected from one or a mixture of two or more of rose violet, rose bengal, fuchsin, carmine, acid scarlet and the like.

The seed coating agent containing chlorantraniliprole, fluopyram and efficient cyhalothrin is used for seed coating treatment during seeding of peanut, potato, corn and wheat crops, prevention and control of seedling stage pests and diseases and insect pests in underground and prevention and control of grubs, mole crickets, wireworms, cutworms and nematodes.

The invention has the advantages that:

1. at present, the prevention and control of plant diseases and insect pests of peanut, potato, corn and wheat crops in the prior art are mainly in a leaf surface spraying mode, the prevention and control of the plant diseases and insect pests can be effectively controlled through multiple pesticide application, manpower and material resources are consumed, the drug resistance of the plant diseases and insect pests is easily increased, and environmental pollution is easily caused. Under the new situation of new policy, people take green, labor-saving, sustainable development and improvement of pesticide utilization rate as a guide. The seed coating agent containing chlorantraniliprole, fluopyram and efficient cyhalothrin can effectively control plant diseases and insect pests by one-time seed treatment, so that the duration is prolonged, the pesticide dosage and the use times are reduced, the plant disease and insect pest control cost is reduced, the labor force is saved, the environmental pollution is reduced, and the use rate of pesticides is improved;

2. the composition containing chlorantraniliprole, fluopyram and lambda-cyhalothrin has a synergistic effect;

3. the composition containing chlorantraniliprole, fluopyram and lambda-cyhalothrin has small dosage and is not easy to generate resistance;

4. the composition containing chlorantraniliprole, fluopyram and efficient cyhalothrin is green and safe, is safe to crops and natural enemies of pests, is safe to environment and human beings, reduces environmental pollution and reduces pesticide residues of agricultural products.

Detailed Description

The technical solutions in the embodiments of the present invention are described in detail below, and the described embodiments do not represent all embodiments. All other embodiments obtained by a person skilled in the art without making any inventive step are within the scope of protection of the present invention.

Formulation example 1: 3% chlorantraniliprole, fluopyram and efficient cyhalothrin seed treatment dispersible powder

1% of chlorantraniliprole, 1% of fluopyram, 1% of lambda-cyhalothrin, 6% of dispersing agent, 3% of wetting agent, 4% of film-forming agent, 3% of warning color and 100% of filler, uniformly mixing in a mixer, crushing by a jet mill, and uniformly mixing in the mixer to obtain the pesticide.

Formulation example 2: dispersible powder for treating 7% chlorantraniliprole, fluopyram and efficient cyhalothrin seeds

1% of chlorantraniliprole, 1% of fluopyram, 5% of high-efficiency cyhalothrin, 8% of dispersing agent, 2% of wetting agent, 2% of film-forming agent, 3% of warning color and 100% of filler, uniformly mixing in a mixer, crushing by a jet mill, and uniformly mixing in the mixer to obtain the pesticide.

Formulation example 3: 14% chlorantraniliprole, fluopyram and efficient cyhalothrin seed treatment dispersible powder

2% of chlorantraniliprole, 2% of fluopyram, 10% of lambda-cyhalothrin, 6% of a dispersant, 4% of a wetting agent, 3% of a film-forming agent, 2% of a warning color and the balance of a filler to 100%. Mixing uniformly in a mixer, crushing by a jet mill, and mixing uniformly in the mixer to obtain the product.

Formulation example 4: 10% chlorantraniliprole, fluopyram and efficient cyhalothrin seed treatment dispersible powder

1% of chlorantraniliprole, 1% of fluopyram, 8% of high-efficiency cyhalothrin, 6% of dispersing agent, 3% of wetting agent, 4% of film-forming agent, 3% of warning color and 100% of filler, uniformly mixing in a mixer, crushing by a jet mill, and uniformly mixing in the mixer to obtain the pesticide.

Formulation example 5: dispersible powder for seed treatment of 20% chlorantraniliprole, fluopyram and efficient cyhalothrin

2% of chlorantraniliprole, 2% of fluopyram, 16% of high-efficiency cyhalothrin, 8% of dispersing agent, 2% of wetting agent, 2% of film forming agent, 3% of warning color and 100% of filler, uniformly mixing in a mixer, crushing by a jet mill, and uniformly mixing in the mixer to obtain the pesticide.

Formulation example 6: 12% chlorantraniliprole, fluopyram and efficient cyhalothrin seed treatment dispersible powder

1% of chlorantraniliprole, 10% of fluopyram, 1% of lambda-cyhalothrin, 6% of a dispersant, 4% of a wetting agent, 3% of a film-forming agent, 2% of a warning color and the balance of a filler to 100%. Mixing uniformly in a mixer, crushing by a jet mill, and mixing uniformly in the mixer to obtain the product.

Formulation example 7: 19% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

Dispersing agent 4%, antifreezing agent 4%, thickening agent 3%, defoaming agent 0.5%, film-forming agent 1%, warning colour 3%, water supplementing 100%, high-speed shearing and uniformly mixing them, adding chlorantraniliprole 1%, fluopyram 10% and high-effective cyhalothrin 8%, and sanding for 2-3 hr in sand mill to make grain size be below 5 micrometers.

Formulation example 8: 12% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

5% of dispersing agent, 3% of antifreezing agent, 3% of thickening agent, 0.5% of defoaming agent, 3% of film-forming agent, 3% of warning color and 100% of water are complemented, and the components are uniformly mixed by high-speed shearing, 10% of chlorantraniliprole, 1% of fluopyram and 1% of efficient cyhalothrin are sequentially added, and the mixture is sanded in a sand mill for 2-3 hours to make the particle size below 5 microns.

Formulation example 9: 24% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

5% of dispersing agent, 5% of antifreezing agent, 2% of thickening agent, 0.5% of defoaming agent, 2% of film-forming agent, 4% of warning color and water for supplementing 100%, uniformly mixing by high-speed shearing, sequentially adding 20% of chlorantraniliprole, 2% of fluopyram and 2% of high-efficiency cyhalothrin, and sanding for 2-3 hours in a sand mill to make the particle size below 5 microns.

Formulation example 10: 16% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

Dispersing agent 4%, antifreezing agent 4%, thickening agent 3%, defoaming agent 0.5%, film-forming agent 1%, warning colour 3%, water supplementing 100%, high-speed shearing and uniformly mixing them, adding 10% of chlorantraniliprole, 1% of fluopyram and 5% of high-effective cyhalothrin, and sanding them in sand mill for 2-3 hr to make the grain size be below 5 micrometers.

Formulation example 11: 17% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

5% of dispersing agent, 3% of antifreezing agent, 3% of thickening agent, 0.5% of defoaming agent, 3% of film-forming agent, 3% of warning color and water for supplementing 100%, uniformly mixing by high-speed shearing, sequentially adding 15% of chlorantraniliprole, 1% of fluopyram and 1% of high-efficiency cyhalothrin, and sanding for 2-3 hours in a sand mill to make the particle size below 5 microns.

Formulation example 12: 21% chlorantraniliprole, fluopyram and efficient cyhalothrin suspension seed coating agent

5% of dispersing agent, 5% of antifreezing agent, 2% of thickening agent, 0.5% of defoaming agent, 2% of film-forming agent, 4% of warning color and water for complementing 100%, uniformly mixing through high-speed shearing, sequentially adding 15% of chlorantraniliprole, 1% of fluopyram and 5% of efficient cyhalothrin, and sanding for 2-3 hours in a sand mill to ensure that the particle size is below 5 microns.

Formulation example 13: dispersible granule for seed treatment of 24% chlorantraniliprole, fluopyram and efficient cyhalothrin

15% of chlorantraniliprole, 1% of fluopyram, 8% of lambda-cyhalothrin, 8% of dispersing agent, 3% of wetting agent, 15% of disintegrating agent, 3% of film-forming agent, 4% of warning color and 100% of filler. Mixing in a mixer, jet milling, adding into a fluidized bed granulator or adding water, kneading, extruding, granulating, drying, and sieving.

Formulation example 14: dispersible granule for treating seeds with 26% chlorantraniliprole, fluopyram and efficient cyhalothrin

15% of chlorantraniliprole, 10% of fluopyram, 1% of lambda-cyhalothrin, 5% of a dispersing agent, 5% of a wetting agent, 10% of a disintegrating agent, 2% of a film forming agent, 3% of a warning color and the balance of a filler to 100%. Mixing in a mixer, jet milling, adding into a fluidized bed granulator or adding water, kneading, extruding, granulating, drying, and sieving.

Formulation example 15: dispersible granule for treating 33% chlorantraniliprole, fluopyram and efficient cyhalothrin seeds

15% of chlorantraniliprole, 10% of fluopyram, 8% of lambda-cyhalothrin, 6% of dispersing agent, 3% of wetting agent, 20% of disintegrating agent, 3% of film-forming agent, 1% of warning color and 100% of filler. Mixing in a mixer, jet milling, adding into a fluidized bed granulator or adding water, kneading, extruding, granulating, drying, and sieving.

The experiment adopts a method combining indoor toxicity measurement and field test. Firstly, determining the co-toxicity coefficient (CTC) of three medicaments compounded according to a certain proportion through indoor toxicity measurement, wherein the CTC is not more than 80 and is an antagonistic action, the CTC is not less than 80 and is not more than 120 and is an additive action, and the CTC is not less than 120 and is a synergistic action, and then carrying out field test on the basis.

Measured virulence index (ATI) ═ standard agent EC₅₀Test drug efficacy EC₅₀)×100；

Theoretical virulence factor (TTI) ═ virulence index of a agent × percentage of a in the mixture + virulence index of B agent × percentage of B in the mixture;

co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

The indoor toxicity test adopts an insect soaking method, wherein a chlorantraniliprole technical, a fluopicolide technical and efficient cyhalothrin are prepared according to a certain proportion and diluted into 5 concentrations for toxicity test, and LC is calculated according to the data obtained by the test₅₀And according to LC₅₀Calculating the toxicity index and co-toxicity coefficient.

The pests needed by indoor bioassay are all manually captured in the occurrence period of the pests, the needed pests are grubs, mole cricket, cutworms and wireworm adults, and different adults are treated before experiments.

Grub: transferring the adults into a sandy loam container filled with disinfection and moisture preservation, wherein the sandy loam contains 17% of water and is 10cm thick, sealing by using a gauze, feeding the adults at 25 ℃ by using apple tree branches as feed, changing the feed every other day, and collecting worm eggs in the soil. The eggs are put into a culture dish filled with disinfected sandy loam with the water content of 10-20%, a cover is covered, and the development condition of the eggs is observed. Transferring the newly hatched larva into an insect culturing box filled with sterile sandy loam with the water content of 15%, culturing at 25 ℃ for one-head, and culturing with malt to 2 years old. Selecting larvae with similar initial health status and head size at 2-year (3d-5d) with consistent incubation days for experiment, soaking into 5 medicinal liquids with different concentrations at each ratio for 10s, sucking off excessive medicinal liquid with filter paper, repeating for 4 times, soaking 15 heads of insects for each time, and setting 4 blank controls without medicament treatment. The treated larvae were transferred to a glass tube containing fresh peanut leaves as food, the mouth of the tube was covered with a moist black cloth, and the tube was kept at 25 ℃ and 70% humidity for observation.

Mole cricket: transferring the imagoes into a sandy loam container filled with disinfection and moisture preservation, wherein the sandy loam contains 17% of water and 10cm of thickness, sealing by a gauze, feeding the sandy loam at 25 ℃ by taking wheat for accelerating germination as a feed, changing the feed every other day, and collecting egg chambers in the soil once every 5 days. Placing the egg chambers into a pest breeding box filled with disinfected sandy loam with the water content of 15%, making a plurality of soil nests on the soil surface, placing one egg chamber in each nest, covering the egg chambers with covers, and observing the development condition of the eggs of the insects until the insects of 3 ages leave the egg chambers. Transferring into insect-culturing box filled with sterilized sandy loam with water content of 15%, and feeding with fructus Hordei Germinatus. Selecting larvae with similar initial health status and head size at 3 ages (2d-3d) with consistent incubation days for experiment, soaking into 5 medicinal liquids with different concentrations at each ratio for 10s, sucking off excessive medicinal liquid with filter paper, repeating for 4 times, soaking 15 heads of insects for each time, and setting 4 blank controls without medicament treatment. Transferring the treated mole cricket to a sandy loam insect-breeding box with the water content of 17% for breeding, putting malt in the box for breeding, and breeding and observing under the conditions that the temperature is 25 ℃ and the humidity is 70%.

The cutworm: transferring the imagoes into an insect breeding cage, and breeding at 25 ℃ by taking honey liquid as nutrient substances. White paper is put into the insect cage for spawning, and insect eggs are collected once a day. Hatching the collected ova at 25 deg.c, and raising the hatched larva in an insect raising box to 3 years old. Selecting larvae with identical incubation days at 3 rd age and similar initial health status and head size, soaking in 5 medicinal liquids with different concentrations at each ratio for 10s, removing excessive medicinal liquid with filter paper, repeating for 4 times, soaking 15 heads for each time, and setting 4 blank controls without medicament treatment. The treated larvae were transferred to a glass tube with fresh peanut leaves and were kept at 25 ℃ and 70% humidity for observation.

Golden needle worm: transferring the imagoes into a sandy loam container filled with disinfection and moisture preservation, wherein the sandy loam contains 17% of water and 10cm of thickness, sealing with a gauze, feeding at 25 ℃ by taking fresh wheat seedlings as feed, picking out the eggs from the roots of the wheat seedlings, and putting the eggs into a small-sized feeding box for hatching at 25 ℃. Transferring the newly hatched larvae into an insect breeding box filled with sterilized sandy loam with the water content of 15%, and feeding the larvae to 3 years old by using malt. Selecting larvae with identical incubation days at 3 rd age and similar initial health status and head size, soaking in 5 medicinal liquids with different concentrations at each ratio for 10s, removing excessive medicinal liquid with filter paper, repeating for 4 times, soaking 15 heads for each time, and setting 4 blank controls without medicament treatment. Transferring the treated golden yellow croaker to a sandy loam insect-breeding box with the water content of 17% for breeding, putting malt in the box for breeding, and breeding and observing under the conditions that the temperature is 25 ℃ and the humidity is 70%.

The method of inspection and statistical analysis was as follows:

and investigating the death condition of the test insects 72 hours after treatment, wherein the judgment standard is that the insects shrink obviously or the needles cannot climb normally, and recording the total number of the insects and the number of the dead insects.

Mortality (%). mortality vs. number of treated insects x 100

Corrected mortality (%) - (treatment mortality-blank mortality)/(1-blank mortality) × 100

Control mortality < 5%, no correction required; the control mortality rate is 5% -20%, and needs to be corrected; control mortality was > 20%, and the test was redone.

Indoor toxicity tests for grubs, mole cricket, cutworm and wireworm were carried out according to the above test methods, and the test results are shown in tables 1, 2, 3 and 4.

Bioassay example 1

TABLE 1 toxicity test results of different proportions of chlorantraniliprole, fluopyram and lambda-cyhalothrin for grubs

From the measurement results table 1, it can be seen that: LC of chlorantraniliprole, fluopyram and lambda-cyhalothrin against grubs₅₀Respectively 5.84mg/L, 13.24mg/L and 2.28mg/L, has good insecticidal effect on white grubs after being mixed according to the mass ratio of the effective components of 1-15:1-10:1-8, has the co-toxicity coefficient (CTC) of more than 120, and shows good synergistic effect.

Bioassay example 2

TABLE 2 toxicity test results of different ratios of chlorantraniliprole, fluopyram and lambda-cyhalothrin to mole cricket

From the measurement results table 2, it can be seen that: LC of chlorantraniliprole, fluopyram and lambda-cyhalothrin on mole cricket₅₀Respectively 8.13mg/L, 17.88mg/L and 3.45mg/L, and has good insecticidal effect on mole cricket after being mixed according to the mass ratio of the effective components of 1-15:1-10:1-8, the Common Toxicity Coefficient (CTC) is more than 120, and the good synergistic effect is shown.

Bioassay example 3

TABLE 3 toxicity test results of cutworms with different proportions of chlorantraniliprole, fluopyram and lambda-cyhalothrin

From the measurement results table 3, it can be seen that: LC of chlorantraniliprole, fluopyram and lambda-cyhalothrin against cutworm₅₀The insecticidal composition is respectively 6.74mg/L, 12.49mg/L and 2.78mg/L, has good insecticidal effect on cutworms after being mixed according to the mass ratio of the effective components of 1-15:1-10:1-8, has the co-toxicity coefficient (CTC) of more than 120, and shows good synergistic effect.

Bioassay example 4

TABLE 4 toxicity test results of different proportions of chlorantraniliprole, fluopyram and lambda-cyhalothrin for golden insects

From the measurement results table 4, it can be seen that: LC of chlorantraniliprole, fluopyram and lambda-cyhalothrin for golden insects₅₀4.93mg/L, 10.88mg/L and 2.96mg/L respectively, has good insecticidal effect on the wireworms after being mixed according to the mass ratio of the effective components of 1-15:1-10:1-8, has the co-toxicity coefficient (CTC) of more than 120, and shows good synergistic action.

The present invention is further illustrated below in connection with field efficacy testing.

The field pesticide effect is carried out from two aspects of indoor germination rate and pest control effect, and the investigation method comprises the following steps:

taking 100 seeds for each treatment, and measuring the germination rate, wherein the indoor germination rate (%) -the total number of the germinated seeds/seed dressing seeds is multiplied by 100%;

observing whether the phytotoxicity is generated from the emergence of seedlings to the end of the growth period;

and (3) performing underground pest investigation at a 3-4 leaf stage after seed emergence, recording seedlings bitten or bitten by mole crickets, grubs, cutworms or wireworms as insect-damaged seedling number, and calculating insect prevention effect, wherein the insect-damaged seedling rate (%) (insect-damaged seedling rate in a control area-insect-damaged seedling rate in a treatment area)/insect-damaged seedling rate in the control area (multiplied by 100 percent).

Bioassay example 5

Formulation example 1, formulation example 3, formulation example 5 and formulation example 7 were mixed with 20 g/acre of the active ingredient without treatment, and 100 peanut seeds were each subjected to germination rate test, the test results of which are shown in table 5:

TABLE 5 determination results of peanut germination rate of seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin

As can be seen from Table 5, the coating treatment of the seed coating agent had no significant effect on the germination of the peanuts and was safe during the germination of the seeds.

The experiment is carried out according to the dosage of 20 g/mu of the active ingredient in the preparation example, and the control agents are 15% of chlorantraniliprole suspending agent, 8% of high-efficiency cyhalothrin suspending agent and 10.4% of chlorantraniliprole-high-efficiency cyhalothrin suspending agent (80 g and 150g of the 15% of the chlorantraniliprole suspending agent and the 8% of the high-efficiency cyhalothrin suspending agent are respectively mixed to prepare the suspending agent with 1:1 of the active ingredient).

The experimental area is 66.7m per cell²Repeating the steps for 3 times; the investigation method of the prevention effect after the pesticide is applied comprises the following steps: randomly sampling 5 points in the experimental treatment area, investigating the number of the insect-damaged seedlings according to the relevant standards of the national field test, and calculating the prevention effect.

The experiment of the invention is carried out in Zhengyang county of Juma shop, Henan province in 2017. The control target is peanut soil insects, and the control effect is shown in table 6.

TABLE 6 field test results of seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin for peanut and underground pests

The test results from table 6 show that: the seed coating agent compounded by the chlorantraniliprole, the fluopyram and the efficient cyhalothrin has excellent effect on controlling the underground pests of the peanuts, has remarkable control effect compared with two single agents and a compound preparation of the two single agents, and shows that the compound of the three effective components has good synergistic effect. In the test process, no phytotoxicity of the preparation to peanut seedlings is found, and the compound seed coating agent is safe to use for peanut crops.

Bioassay example 6

Formulation example 2, formulation example 4, formulation example 6 and formulation example 8 were mixed with 20 g/acre of the active ingredient without treatment, 100 potatoes were each subjected to germination rate test, and the test results are shown in table 7:

TABLE 7 seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin for determining germination rate of potato

Treatment of	Percentage of germination (%)
		Formulation example 2	94
Formulation example 4	92
		Formulation example 6	95
Formulation example 8	94
		Control	94

As can be seen from Table 7, the coating treatment of the seed coating had no significant effect on the germination of the potatoes and was safe during the germination of the seeds.

The experiment of the invention was carried out in 2017 in Longxi county, Dengxi City, Gansu province. The control target is potato soil insects, and the control effect is shown in table 8.

TABLE 8 field test results of seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin for potato soil insects

The test results from table 8 show that: the seed coating agent compounded by the chlorantraniliprole, the fluopyram and the high-efficiency cyhalothrin has excellent effect on controlling the potato soil insects, has remarkable control effect compared with two single agents and a compound preparation of the two single agents, and shows that the compound of the three effective components has good synergistic effect. In the test process, no phytotoxicity of the preparation to potato seedlings is found, and the compound seed coating agent is safe to use for potato crops.

Bioassay example 7

Formulation example 9, formulation example 11, formulation example 13 and formulation example 15 were mixed with 20 g/acre of the active ingredient without treatment, and 100 grains of corn were each subjected to germination rate test, the test results of which are shown in table 9:

TABLE 9 measurement results of germination rate of corn by using seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin

Treatment of	Percentage of germination (%)
		Formulation example 9	93
Formulation example 11	92
		Formulation example 13	95
Formulation example 15	94
		Control	93

As can be seen from Table 9, the coating treatment of the seed coating agent had no significant effect on the germination of corn and was safe during the germination of the seeds.

The experimental area is 66.7m per cell²Repeating the steps for 3 times; the investigation method of the prevention effect after the pesticide is applied comprises the following steps: in thatRandomly sampling 5 points in the experimental treatment area, investigating the number of the insect-damaged seedlings according to the relevant standards of the national field test, and calculating the prevention effect.

The experiment of the invention is carried out in 2017 in Yongji county, Jilin City, Jilin province. The control target is corn underground insects, and the control effect is shown in table 10.

TABLE 10 field test results of seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin for corn soil insects

The test results from table 10 show that: the seed coating agent compounded by the chlorantraniliprole, the fluopyram and the high-efficiency cyhalothrin has excellent effect on controlling the underground pests of the corns, has remarkable control effect compared with two single agents and a compound preparation of the two single agents, and shows that the compound of the three effective components has good synergistic effect. In the test process, no phytotoxicity of the preparation to corn seedlings is found, and the compound seed coating agent is safe to use for corn crops.

Bioassay example 8

Formulation example 10, formulation example 12 and formulation example 14 were mixed with 20 g/acre of the active ingredient without treatment, and 100 wheat grains were each subjected to germination rate test, the test results of which are shown in table 11:

TABLE 11 measurement results of germination rate of wheat by seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin

As can be seen from Table 11, the coating treatment of the seed coating agent had no significant effect on the germination of wheat and was safe during the germination of the seeds.

The experiment of the invention is carried out in the Rugao region of Nantong city in Jiangsu province in 2017. The control target is wheat soil insects, and the control effect is shown in table 12.

TABLE 12 field test results of seed coating agent of chlorantraniliprole, fluopyram and lambda-cyhalothrin for wheat soil insects

The test results from table 12 show that: the seed coating agent compounded by the chlorantraniliprole, the fluopyram and the high-efficiency cyhalothrin has excellent effect on controlling the underground pests of the wheat, has remarkable control effect compared with two single agents and a compound preparation of the two single agents, and shows that the compound of the three effective components has good synergistic effect. In the test process, no phytotoxicity of the preparation to wheat seedlings is found, and the compound seed coating agent is safe to use for wheat crops.

According to the above biological test embodiments, the chlorantraniliprole, the fluopyram and the efficient cyhalothrin are compounded according to the mass ratio of 1-15:1-10:1-8 to prepare the suspension seed coating agent, the seed treatment dispersible powder and the seed treatment dispersible granule, so that the pesticide composition has a remarkable control effect on grubs, mole crickets, wireworms and cutworms of underground pests of peanut, potato, corn and wheat crops, has an obvious synergistic effect, reduces the pesticide dosage and the production cost, is safe for the crops, has a long lasting period, saves labor and time, improves the use efficiency of the pesticide, is an ideal pesticide for controlling underground pests of the peanut, potato, corn and wheat crops, and has a wide application prospect in production.

In conclusion, the composition adopts a compounding scheme of three effective components of chlorantraniliprole, fluopyram and efficient cyhalothrin, the insecticidal activity of the composition is not the superposition of the activities of the three simple effective components, and compared with the existing single preparation or the combination of two effective components, the composition has obvious insecticidal effect, obvious synergistic effect, reduced control cost, safety to crops and long duration, and meets the safety requirement of pesticide preparations.

Claims

1. A seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin is characterized in that: the effective components are chlorantraniliprole, fluopyram and lambda-cyhalothrin; the mass ratio of the effective components of the chlorantraniliprole, the fluopyram and the lambda-cyhalothrin is 1-15:1-10: 1-8.

2. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin according to claim 1, which is characterized in that: the mass contents of the effective components of chlorantraniliprole, fluopyram and lambda-cyhalothrin are respectively 1-15%, 1-10% and 1-8%, and the balance is auxiliary components.

3. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin according to claim 1, which is characterized in that: the pesticide preparation which can be prepared from the seed coating agent is a suspension seed coating agent, seed treatment dispersible powder and seed treatment dispersible granules.

4. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin as claimed in any one of claims 1 to 3, which is characterized in that: when the seed coating agent is a suspension seed coating agent, the mass ratio of the components in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 1 to 5 percent of dispersant, 1 to 5 percent of antifreeze, 0.1 to 5 percent of film forming agent, 1 to 5 percent of thickening agent, 0.1 to 2 percent of defoaming agent, 1 to 5 percent of warning color and the balance of water to 100 percent; the specific processing steps are as follows: fully mixing the components in the formula according to the formula, uniformly mixing by high-speed shearing, and sanding in a sand mill for 2-3 hours to ensure that the particle size is below 5 microns, thus obtaining the suspended seed coating agent.

5. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin as claimed in any one of claims 1 to 3, which is characterized in that: when the seed coating agent is dispersible powder, the mass ratio of each component in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 2-8% of dispersing agent, 1-6% of wetting agent, 1-10% of film-forming agent, 1-5% of warning color and 100% of filler; the specific processing steps are as follows: the components in the formula are mixed according to the formula, the mixture is uniformly mixed and stirred in a mixing stirrer, the mixture is crushed by a jet mill, and the seed treatment dispersible powder is obtained after uniform mixing.

6. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin as claimed in any one of claims 1 to 3, which is characterized in that: when the dosage form of the seed coating agent is dispersible granules, the weight ratio of the components in the preparation is 1-15% of chlorantraniliprole, 1-10% of fluopyram, 1-8% of lambda-cyhalothrin, and the auxiliary components are as follows: 1-6% of dispersing agent, 2-6% of wetting agent, 1-10% of disintegrating agent, 1-5% of film-forming agent, 1-5% of warning color and 100% of filler; the specific processing steps are as follows: the components are uniformly mixed according to the formula, are subjected to air flow crushing and then are uniformly mixed, and then are added into a fluidized bed granulator for granulation, or are added with water for kneading and then are subjected to extrusion granulation, drying, screening and sampling analysis, so that the seed treatment dispersible granule is obtained.

7. The seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin as claimed in claim 3, wherein:

the thickening agent is selected from one or a mixture of two or more of xanthan gum, magnesium aluminum silicate, hydroxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, sodium alginate, Arabic gum, polyethylene glycol, polyvinylpyrrolidone, polyacrylamide, sodium polyacrylate and the like;

the film forming agent is one or a mixture of two or more of xanthan gum, hydroxymethyl cellulose, polyvinyl alcohol, sodium alginate, polyvinyl alcohol, Arabic gum, polyacrylamide, polyethylene glycol, polyvinyl acetate, starch and the like;

the antifreezing agent is one or the mixture of two or more of ethylene glycol, propylene glycol, glycerol, urea, ammonium sulfate, sodium sulfate and sodium chloride;

the defoaming agent is one or a mixture of two or more of organic silicon defoaming agents, fatty alcohol compounds and fatty acid compounds;

the warning color is selected from one or a mixture of two or more of rose violet, rose bengal, fuchsin, carmine, acid carmine and the like.

8. The use of the seed coating agent containing chlorantraniliprole, fluopyram and lambda-cyhalothrin according to claim 1 for seed coating treatment during seeding of peanut, potato, corn and wheat crops, for seedling pest control of peanut, potato, corn and wheat crops, and for control of grubs, mole crickets, wireworms and cutworms as underground pests of peanut, potato, corn and wheat crops.