CN107258773A - A kind of screening technique of Monochamus alternatus infochemical slow-release material - Google Patents
A kind of screening technique of Monochamus alternatus infochemical slow-release material Download PDFInfo
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- CN107258773A CN107258773A CN201710242904.9A CN201710242904A CN107258773A CN 107258773 A CN107258773 A CN 107258773A CN 201710242904 A CN201710242904 A CN 201710242904A CN 107258773 A CN107258773 A CN 107258773A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/023—Attracting insects by the simulation of a living being, i.e. emission of carbon dioxide, heat, sound waves or vibrations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/026—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects combined with devices for monitoring insect presence, e.g. termites
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of screening technique of Monochamus alternatus infochemical slow-release material, from the point of view of sustained release rate and attractant component proportion, using weight method and gas chromatography, the slow-release material of Monochamus alternatus infochemical is screened.Test by (+) α firpenes, () beta pinene, the ethanol of 2 hendecane epoxide 1 according to 9: 1: 1 ratio, it is configured to Monochamus alternatus semiochemical mixed liquor, lure, respectively low-density polyethylene material (LDPE), high-density polyethylene material (HDPE), polyethylene pipe (PE) and wick bottle (PP) are used as from 4 kinds of slow-release materials.Test result indicates that, this 4 kinds of slow-release materials have certain advantage and disadvantage on slow release effect.The screening technique of the Monochamus alternatus infochemical slow-release material can provide Technical Reference to develop more economical, efficient Monochamus alternatus attractant.
Description
Technical field
The present invention relates to a kind of screening technique of Monochamus alternatus infochemical slow-release material.
Background technology
Monochamus alternatus (Monochamus alternatus Hope) is the main trunk borer of China pine tree, is individually occurred
Also pine wilt disease can be caused and lethal, is also the main media insect of destructive disease-pine nematode of pine tree.In pine
In the diffusion and infection processs of nematode, the key effect that Monochamus alternatus plays carrying, propagates and assist cause of disease to invade host.Cause
The population quantity and growth trend of this effectively control Monochamus alternatus contribute to protection economic forest pine forest and landscape pine forest.
At present, the preventing and treating for alternatus Larva include that Deceased wood is stifling based on killing, mainly and medicament immersion at
Reason, this method generally wastes time and energy, and is unfavorable for the mass control in forest farm, and the medicament of residual can also cause environmental pollution.
Using trapper and combine efficient attractant and trap and kill Monochamus alternatus, it is not only efficiently single-minded, and ecological, environmental protective, use manpower and material resources sparingly.
The research of insect pheromone is the domestic and international research emphasis for carrying out control of insect with application.Research finds that aggregation is believed
Breath element and botanical attractant, which are combined, has very strong attracting action for Monochamus alternatus imago, in actually preventing and treating, also passes through
Often Monochamus alternatus is trapped and killed using this kind of efficient attractant.But, in practice it has proved that when this kind of lure has preventing and treating persistently
Between it is short, the shortcomings of the big, stability that is affected by the external environment is poor, hinder the large scale application of lure.It is therefore desirable to
In the making of bionical lure, existing slow release method is introduced or improved, suitable slow-released carrier is selected, protection information compound is exempted from
By environment degradable and the release that can maintain a long-term stability, with the maximum bioactivity for playing lure, and then Monochamus alternatus lure is improved
Attractant effect.
Therefore, a kind of screening technique of rational Monochamus alternatus infochemical slow-release material is explored very necessary.
The content of the invention
It is contemplated that at present on the market pesticide control, trapper preventing and treating and the defect of biological control there is provided one kind
Simple environment protection, easy to operate, economical and practical, pest-resistant lure the black longicorn infochemical slow-release material screening of worm continuation prednisone
Method.
The technical scheme that a kind of screening technique of Monochamus alternatus infochemical slow-release material of the present invention is used is：From
4 kinds of slow-release materials are used as lure, respectively low-density polyethylene material (LDPE), high-density polyethylene material (HDPE), poly- second
Alkene pipe (PE) and wick bottle (PP), by (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol according to 9: 1: 1 ratio
Example is configured to Monochamus alternatus semiochemical mixed liquor, and semiochemical mixed liquor is transferred in sustained release bottle with liquid-transfering gun, plus
To bottle mouth position.Polyethylene pipe is smaller due to pore, injects 2mL semiochemical mixed liquors with sample introduction needle, two ends are sealed with lighter
Mouthful.7 groups are set per sample to be tested, wherein 2 groups are used for GC analysis of mixtures ratios, another 5 groups are used for calculating loss amount of weighing.
Field lure laying state is simulated, different carriers lure is hung on into same collision type Monochamus alternatus trap.
The blending constituent is matched：9 parts of (+)-australene, 1 part of (-)-nopinene, 1 part of 2- hendecane epoxide -1- ethanol
The present invention from 4 kinds of slow-release materials by being used as lure, and research Monochamus alternatus semiochemical is in 4 kinds of slow-released carriers
On release mode and release dynamics feature, for Monochamus alternatus infochemical slow-release material screening technique provide with reference to according to
According to.Test result indicates that, these four different slow-released carriers remained to release at 70 days or so, illustrated that these four materials are all in theory
Longer attractant effect can be reached in the wild, but from the point of view of the harmony of material sustained-release, three kinds of Monochamus alternatus semiochemicals
The volatilization ratio for being sustained component in bottle A (LDPE), polyethylene sustained release bottle B (HDPE) and polyethylene pipe (PE) in polyethylene is not assisted
Adjust, i.e., can not preferably keep the initial proportion of three kinds of semiochemicals；From the average daily of four kinds of slow-release material internal information compounds
From the point of view of rate of release, polyethylene sustained release bottle A, polyethylene pipe and wick bottle material experiment semiochemical at initial stage burst size are big, with
Experimental period extends, and burst size reduces (burst size in wick bottle 70 days later stages of experiment is minimum), and this is unfavorable for keeping attractant long
Phase attractant effect, polyethylene sustained release bottle B volatile quantity fluctuation is relatively stable.
Brief description of the drawings
Fig. 1 is four kinds of experiment slow-release materials；
Fig. 2 is that three kinds of semiochemicals are sustained the release dynamics in bottle A (LDPE) in polyethylene；
Fig. 3 is that three kinds of semiochemicals are sustained the release dynamics in bottle B (HDPE) in polyethylene；
Fig. 4 is release dynamics of three kinds of semiochemicals in polyethylene pipe (PE)；
Fig. 5 is release dynamics of three kinds of semiochemicals in wick bottle (PP).
Embodiment
With reference to embodiment, the present invention is described in detail, described Monochamus alternatus infochemical slow-release material
Screening technique comprise the following steps：
Embodiment one
Prepare：By (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol according to 9: 1: 1 proportions into
Monochamus alternatus semiochemical mixed liquor, low-density polyethylene material is transferred to liquid-transfering gun by semiochemical mixed liquor
(LDPE) in sustained release bottle, bottle mouth position is added to.
Determine：7 groups are set to be tested, wherein 2 groups are used for GC analysis of mixtures ratios, another 5 groups are used for calculating damage of weighing
Vector.Field lure laying state is simulated, carrier lure is hung on a collision type Monochamus alternatus trap.Using 7820A
Gas chromatograph (gas chromatograph 7820A, Agilent companies) is analyzed, and chromatographic column is the nonpolar capillaries of DB-5MS
Chromatographic column (30m × 0.25mm × 0.25 μm).Analysis condition：80 DEG C of heating schedule keeps 1min, and then 5 DEG C/min rises to
230 DEG C, kept for 5 minutes.By the 1 of slow-released carrier, No. 2 samples be used to analyze, draw 1 μ L semiochemicals, n-hexane with sample introduction needle
Take 1 μ L dilutions sample introduction to analyze after 1000 times of dilution, all seal the sample tap of slow-released carrier after each GC analyses.Spectrogram is qualitative
Method：(+)-australene, (-)-nopinene and the independent sample introduction of 2- hendecane epoxide -1- ethanol are subjected to GC analyses, three kinds are obtained
The retention time at semiochemical peak, the material peak in mixed information compound is determined according to the retention time of material；Spectrogram is determined
Amount method：Area normalization method progress quantitative analysis is pressed to the attractant of detection, the percentage of each semiochemical is tried to achieve respectively
Content.The 3-7 samples using slow-released carrier are weighed, weight is weighed within every 5 days, records the total amount and loss amount and weather feelings of sample
Condition, is weighed terminate every time, and sample is put back into original position to continue experiment.Duration of experiment is 68 days.
Three kinds of semiochemicals are sustained the release mode in bottle A (LDPE) in polyethylene：Three kinds of semiochemicals are in the early stage
In relatively low emission levels, average daily volatile quantity is significantly improved over time, reach within the 19th day maximum burst size (85.84 ±
2.54mg·d-1), then average daily volatile quantity presents and is remarkably decreased trend, and until the 33rd day, average daily volatile quantity slowly declined, and reaches
To an extremely low release conditions, in the 68th day latter stage of observation, burst size is only (2.57 ± 0.21) mgd-1.By multiple ratio
Relatively analysis (Duncan, α=0.05) find that the 19th day rate of release is significantly higher than other times.Discharging the 8th day, three kinds initial stage
The ratio of semiochemical is close to initial ratio 9: 1: 1, (+)-australene, (-)-nopinene and 2- hendecane epoxide -1- ethanol
Relative amount is respectively 84.45%, 9.18%, 6.35%.Since the 28th day, three kinds of semiochemical components were more notable than occurring
Change, is in particular in that (+)-australene volatile quantity increases, the ratio of 2- hendecane epoxide -1- ethanol is significantly raised, to observation
In the 68th day latter stage, the relative amount of three is 2.20%, 0.07%, 95.82%.Contrast the release mode in 68 days, Song Motian
Ox semiochemical (+)-australene discharges very fast in polyethylene sustained release bottle A, it is impossible to long-term to keep semiochemical initially to have
Effect proportioning.
Embodiment two
Prepare：By (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol according to 9: 1: 1 proportions into
Monochamus alternatus semiochemical mixed liquor, high-density polyethylene material is transferred to liquid-transfering gun by semiochemical mixed liquor
(HDPE) in sustained release bottle, bottle mouth position is added to.
Determine：7 groups are set to be tested, wherein 2 groups are used for GC analysis of mixtures ratios, another 5 groups are used for calculating damage of weighing
Vector.Field lure laying state is simulated, carrier lure is hung on a collision type Monochamus alternatus trap.Using 7820A
Gas chromatograph (gas chromatograph 7820A, Agilent companies) is analyzed, and chromatographic column is the nonpolar capillaries of DB-5MS
Chromatographic column (30m × 0.25mm × 0.25 μm).Analysis condition：80 DEG C of heating schedule keeps 1min, and then 5 DEG C/min rises to
230 DEG C, kept for 5 minutes.By the 1 of slow-released carrier, No. 2 samples be used to analyze, draw 1 μ L semiochemicals, n-hexane with sample introduction needle
Take 1 μ L dilutions sample introduction to analyze after 1000 times of dilution, all seal the sample tap of slow-released carrier after each GC analyses.Spectrogram is qualitative
Method：(+)-australene, (-)-nopinene and the independent sample introduction of 2- hendecane epoxide -1- ethanol are subjected to GC analyses, three kinds are obtained
The retention time at semiochemical peak, the material peak in mixed information compound is determined according to the retention time of material；Spectrogram is determined
Amount method：Area normalization method progress quantitative analysis is pressed to the attractant of detection, the percentage of each semiochemical is tried to achieve respectively
Content.The 3-7 samples using slow-released carrier are weighed, weight is weighed within every 5 days, records the total amount and loss amount and weather feelings of sample
Condition, is weighed terminate every time, and sample is put back into original position to continue experiment.Duration of experiment is 69 days.
Three kinds of semiochemicals are sustained the release mode in bottle B (HDPE) in polyethylene：Three kinds of Monochamus alternatus information chemical combination
Thing has certain fluctuation in the average daily burst size that polyethylene is sustained in bottle B, but fluctuating change is not obvious.Reached most at the 34th day
Big rate of release is (50.48 ± 5.54) mgd-1.Observing the 10th day initial stage, (+)-australene, (-)-nopinene and 2- ten
The relative amount of one alkoxy -1- ethanol was respectively 75.13%, 12.02%, 12.80%, at 60 days or so, and three is relative to be contained
Amount is respectively 12.90%, 1.21%, 83.84%.(+)-australene, relative amount of (-)-nopinene in 10-60 days declines
Rate is respectively 82.83%, and the relative amount climbing of 89.93%, 2- hendecane epoxide -1- ethanol is 555.00%.Period,
First 20 days, (+)-australene relative amount, at 40 days or so, dropped to 23.39% more than 65%.
Embodiment three
Prepare：By (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol according to 9: 1: 1 proportions into
Monochamus alternatus semiochemical mixed liquor, because polyethylene pipe pore is smaller, injects 2mL semiochemicals with sample introduction needle and mixes
Sealed with lighter at liquid, two ends.
Determine：7 groups are set to be tested, wherein 2 groups are used for GC analysis of mixtures ratios, another 5 groups are used for calculating damage of weighing
Vector.Field lure laying state is simulated, carrier lure is hung on a collision type Monochamus alternatus trap.Using 7820A
Gas chromatograph (gas chromatograph 7820A, Agilent companies) is analyzed, and chromatographic column is the nonpolar capillaries of DB-5MS
Chromatographic column (30m × 0.25mm × 0.25 μm).Analysis condition：80 DEG C of heating schedule keeps 1min, and then 5 DEG C/min rises to
230 DEG C, kept for 5 minutes.By the 1 of slow-released carrier, No. 2 samples be used to analyze, draw 1 μ L semiochemicals, n-hexane with sample introduction needle
Take 1 μ L dilutions sample introduction to analyze after 1000 times of dilution, all seal the sample tap of slow-released carrier after each GC analyses.Spectrogram is qualitative
Method：(+)-australene, (-)-nopinene and the independent sample introduction of 2- hendecane epoxide -1- ethanol are subjected to GC analyses, three kinds are obtained
The retention time at semiochemical peak, the material peak in mixed information compound is determined according to the retention time of material；Spectrogram is determined
Amount method：Area normalization method progress quantitative analysis is pressed to the attractant of detection, the percentage of each semiochemical is tried to achieve respectively
Content.The 3-7 samples using slow-released carrier are weighed, weight is weighed within every 5 days, records the total amount and loss amount and weather feelings of sample
Condition, is weighed terminate every time, and sample is put back into original position to continue experiment.Duration of experiment is 68 days.
Release mode of three kinds of semiochemicals in polyethylene pipe (PE)：Three kinds of Monochamus alternatus semiochemicals are in poly- second
Average daily rate of release in alkene pipe is in the trend being gradually reduced.Three kinds of semiochemicals just reach release when showing initial release
Peak, rate of release declines rapidly within the 4-14 days, and hereafter average daily rate of release slows down, and low rate of release shape is kept after the 33rd day
State.Analyze by Multiple range test (Duncan, α=0.05) find the 43-68 days, rate of release the change of divergence is not notable, and the 4th, 8
Its average rate of release is higher than other testing times, respectively 120.10 ± 8.17mgd-1, 70.96 ± 5.49mgd-1.Three
Plant Monochamus alternatus semiochemical relative amount variable quantity in polyethylene pipe larger.8th day or so, (+)-australene, (-)-
Nopinene and 2- hendecane epoxide -1- ethanol relative amounts are respectively 82.27%, 8.52%, 9.14%, the 68th day, San Zhexiang
It is respectively 37.49%, 6.62%, 53.63% to content, (+)-australene, the relative amount rate of descent difference of (-)-nopinene
For 54.43%, the relative amount climbing of 22.30%, 2- hendecane epoxide -1- ethanol is up to 486.76%.Period, first 20 days
Left and right, (+)-australene accounts for component ratio more than 75%, and 2- hendecane epoxide -1- ethanol accounts for component ratio below 15%, but
At 40 days or so, the component ratio that (+)-australene relative amount have decreased to 49.10%, 2- hendecane epoxide -1- ethanol reached
To more than 40%.
Example IV
Prepare：By (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol according to 9: 1: 1 proportions into
Monochamus alternatus semiochemical mixed liquor, is transferred to wick bottle (PP) by semiochemical mixed liquor with liquid-transfering gun and is sustained in bottle,
Add to bottle mouth position.
Determine：7 groups are set to be tested, wherein 2 groups are used for GC analysis of mixtures ratios, another 5 groups are used for calculating damage of weighing
Vector.Field lure laying state is simulated, carrier lure is hung on a collision type Monochamus alternatus trap.Using 7820A
Gas chromatograph (gas chromatograph 7820A, Agilent companies) is analyzed, and chromatographic column is the nonpolar capillaries of DB-5MS
Chromatographic column (30m × 0.25mm × 0.25 μm).Analysis condition：80 DEG C of heating schedule keeps 1min, and then 5 DEG C/min rises to
230 DEG C, kept for 5 minutes.By the 1 of slow-released carrier, No. 2 samples be used to analyze, draw 1 μ L semiochemicals, n-hexane with sample introduction needle
Take 1 μ L dilutions sample introduction to analyze after 1000 times of dilution, all seal the sample tap of slow-released carrier after each GC analyses.Spectrogram is qualitative
Method：(+)-australene, (-)-nopinene and the independent sample introduction of 2- hendecane epoxide -1- ethanol are subjected to GC analyses, three kinds are obtained
The retention time at semiochemical peak, the material peak in mixed information compound is determined according to the retention time of material；Spectrogram is determined
Amount method：Area normalization method progress quantitative analysis is pressed to the attractant of detection, the percentage of each semiochemical is tried to achieve respectively
Content.The 3-7 samples using slow-released carrier are weighed, weight is weighed within every 5 days, records the total amount and loss amount and weather feelings of sample
Condition, is weighed terminate every time, and sample is put back into original position to continue experiment.Duration of experiment is 69 days.
Release mode of three kinds of semiochemicals in wick bottle (PP)：For the calculating discovery of loss amount in wick bottle,
In first test in the 5th day, burst size was higher, and then rate of release declined rapidly up to the 10th day, then until observation latter stage,
The burst size of three kinds of semiochemicals is constantly in extremely low emission levels.Three are found when carrying out the analysis of Duncan Multiple range tests
Semiochemical is planted to be substantially less than the 34th day (P ＜ 0.05) in the rate of release of the 69th day；Rate of release is to the maximum within 5th day
24.11±0.56mg·d-1.But because the material is in itself without slow release effect, its sustained release fashion depends on cotton lamp cord, the material
Overall release rate it is relatively low.At the 10th day or so, (+)-australene, (-)-nopinene and 2- hendecane epoxide -1- ethanol
Relative amount is respectively 85.69%, 9.28%, 3.75%, and the 60th day or so, three's relative amount was respectively 85.67%,
9.23%, 5.06%.(+)-australene, the relative amount rate of descent of (-)-nopinene is respectively 0.02%, 0.54%, 2- 11
The relative amount climbing of alkoxy -1- ethanol is 34.93%.The change of three's component ratio is not obvious.
The inventive method illustrates that these four slow-release materials can reach longer attractant effect in the wild in theory, but from
From the point of view of the harmony of material sustained-release, three kinds of Monochamus alternatus semiochemicals are in polyethylene sustained release bottle A (LDPE), polyethylene sustained release bottle
The volatilization ratio of component is uncoordinated in B (HDPE) and polyethylene pipe (PE), i.e., can not preferably keep three kinds of semiochemicals just
Beginning ratio；From the point of view of the average daily rate of release of four kinds of slow-release material internal information compounds, polyethylene sustained release bottle A, polyethylene pipe
And wick bottle material experiment semiochemical at initial stage burst size is big, extends with experimental period, burst size reduces (after wick bottle experiment
The burst size of 70 days phases is minimum), this is unfavorable for keeping the long-term attractant effect of attractant, polyethylene sustained release bottle B volatile quantity fluctuation
Relatively stable, the smaller reprinting amount of polyethylene pipe is to hinder the problem of it is applied.
The beneficial effects of the invention are as follows：From the point of view of sustained release rate and attractant component proportion, using weight method and
Gas chromatography, is screened to the slow-release material of Monochamus alternatus infochemical, for work out it is more economical, can
The Monochamus alternatus high-efficiency sustained-release type attractant of wide popularization and application provides reliable reference.
The specific embodiment of the present invention is these are only, protection scope of the present invention is not limited with this；Do not violating this hair
Any replacement made on the basis of bright design belongs to protection scope of the present invention with improving.
Claims (5)
1. a kind of screening technique of Monochamus alternatus (Monochamus alternatus Hope) infochemical slow-release material,
From (+)-australene, (-)-nopinene, 2- hendecane epoxide -1- ethanol, attractant is configured to by 9: 1: 1 composition ratio, is gathered
Ethene sustained release bottle A (LDPE), polyethylene sustained release bottle B (HDPE), polyethylene pipe (PE) and wick bottle (PP) are believed as Monochamus alternatus
The slow-release material of compound is ceased, Monochamus alternatus lure is fabricated to and hangs on outdoor, the weight of lure is weighed per 5d and to sustained release material
Attractant composition in material carries out GC analyses, Continuous Observation record 70d or so, probes into Monochamus alternatus semiochemical slow at 4 kinds
The release mode and release dynamics feature on carrier are released, suitable slow releasing carrier material is found, and then improve Monochamus alternatus lure
Attractant effect.
2. the screening technique of Monochamus alternatus infochemical slow-release material according to claim 1, it is characterised in that institute
State the aggregation pheromone 2- ten that attractant produces for plant source semiochemical (+)-australene, (-)-nopinene and Monochamus alternatus
One alkoxy -1- ethanol is formed in 9: 1: 1 ratio mixed preparing.
3. the screening technique of Monochamus alternatus infochemical slow-release material according to claim 1, it is characterised in that institute
Slow-release material is stated for low-density polyethylene material (LDPE), high-density polyethylene material (HDPE), polyethylene pipe (PE) and wick
Bottle (PP).
4. the screening technique of Monochamus alternatus infochemical slow-release material according to claim 1, it is characterised in that institute
The method of selection is weight method and gas-chromatography (GC) analytic approach respectively, and weight method mainly determines pine ink day in different slow-release materials
The rate of release of ox semiochemical, gas chromatography predominantly detects (+)-australene, (-)-nopinene and 2- hendecanes
Whether three kinds of semiochemical rates of release of Oxy-1-ethanol are synchronous, as time went on, three kinds of chemistry in remaining attractant
Whether the ratio of material has a greater change.
5. the screening technique of Monochamus alternatus infochemical slow-release material according to claim 1, it is characterised in that institute
The screening technique of Monochamus alternatus infochemical slow-release material is stated, can be that research is more economical, can be widely used
Monochamus alternatus high-efficiency sustained-release type attractant provide reference frame, so as to improve the trapping efficiency of Monochamus alternatus.
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| CN109645003A (en) * | 2019-01-19 | 2019-04-19 | 杭州科森农化有限公司 | A kind of Monochamus alternatus Hope pheromone attractant |
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| CN109645003A (en) * | 2019-01-19 | 2019-04-19 | 杭州科森农化有限公司 | A kind of Monochamus alternatus Hope pheromone attractant |
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