CN110122487A - Alcohol is luring application and a kind of diaphorina citri attractant in diaphorina citri - Google Patents

Alcohol is luring application and a kind of diaphorina citri attractant in diaphorina citri Download PDF

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Publication number
CN110122487A
CN110122487A CN201910435422.4A CN201910435422A CN110122487A CN 110122487 A CN110122487 A CN 110122487A CN 201910435422 A CN201910435422 A CN 201910435422A CN 110122487 A CN110122487 A CN 110122487A
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China
Prior art keywords
diaphorina citri
alcohol
present
cyclohexanediol
octyl alcohol
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CN201910435422.4A
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Chinese (zh)
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CN110122487B (en
Inventor
彭婷
刘新军
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Gannan Normal University
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Gannan Normal University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/14Catching by adhesive surfaces
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/06Oxygen or sulfur directly attached to a cycloaliphatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M2200/00Kind of animal
    • A01M2200/01Insects
    • A01M2200/012Flying insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides alcohol to lure application and a kind of diaphorina citri attractant in diaphorina citri, belongs to plant pest management technical field.The present invention lures diaphorina citri with n-octyl alcohol or anti-form-1,2- cyclohexanediol, lures high-efficient.And the present invention does not need to add other inducing substances that efficiently luring to diaphorina citri can be realized, simple and effective is easy to implement.Embodiment statistics indicate that, the present invention in, n-octyl alcohol lures efficiency to be up to 49.28% to diaphorina citri, and anti-form-1,2- cyclohexanediol lures efficiency to be up to 47.50% to diaphorina citri.

Description

Alcohol is luring application and a kind of diaphorina citri attractant in diaphorina citri
Technical field
The present invention relates to plant pest management technical field more particularly to alcohol lure application in diaphorina citri and A kind of diaphorina citri attractant.
Background technique
Plant-feed insect is by distinguishing that the special odor of contained secondary metabolites in host plant carries out feeding, these are special Different smell is the signaling molecule that insect finds host plant.Diaphorina citri (Diphorin itri Kuwym) is that yellow twig is caused a disease The primary vehicle of bacterium.Positive applying pesticide can control diaphorina citri, it is also possible to cause drug resistance, environmental pollution, Pesticide residue endangers the problems such as natural enemy.Therefore, many environmental-friendly diaphorina citri preventing control methods are developed, as essential oil, Botanical secondary metabolite, parasitic wasp and insect pathogenic fungus and Yellow sticky trap.But effect is lured in the presence of these methods The low problem of rate.
Summary of the invention
In consideration of it, the purpose of the present invention is to provide alcohol lure in diaphorina citri application and a kind of diaphorina citri draw Lure.The present invention lures diaphorina citri using alcohol, lures high-efficient.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of alcohol to lure the application in diaphorina citri, and the alcohol is n-octyl alcohol or anti-form-1,2- ring Hexylene glycol.
Preferably, the n-octyl alcohol uses as a solution, and the solution includes n-octyl alcohol and mineral oil.
Preferably, the volumetric concentration of n-octyl alcohol is 0.1~10% in the solution.
Preferably, the anti-form-1,2- cyclohexanediol use in form of an aqueous solutions.
Preferably, anti-form-1 in the aqueous solution, the volumetric concentration of 2- cyclohexanediol are 0.1~10%.
Preferably, the time lured is >=10min.
Preferably, it is described application the following steps are included:
The n-octyl alcohol is coated on mythimna separata Huang plate, then diaphorina citri is lured.
The present invention also provides a kind of diaphorina citri attractants, including alcohol and solvent, described when the alcohol is n-octyl alcohol Solvent is mineral oil, and when the alcohol is anti-form-1, when 2- cyclohexanediol, the solvent is water.
Preferably, the volumetric concentration of alcohol is 0.1~10% in the diaphorina citri attractant.
The present invention provides alcohol to lure the application in diaphorina citri, and the alcohol is n-octyl alcohol or anti-form-1,2- hexamethylene two Alcohol.The present invention lures diaphorina citri with n-octyl alcohol or anti-form-1,2- cyclohexanediol, lures high-efficient.And the present invention does not need to add Add other inducing substances that efficiently luring to diaphorina citri can be realized, simple and effective is easy to implement.The tables of data of embodiment Bright, in the present invention, n-octyl alcohol lures efficiency to be up to 49.28% to diaphorina citri, and anti-form-1,2- cyclohexanediol is to diaphorina citri Lure efficiency to be up to 47.50%.
Specific embodiment
The present invention provides alcohol to lure the application in diaphorina citri, and the alcohol is n-octyl alcohol or anti-form-1,2- hexamethylene two Alcohol.
In the present invention, the n-octyl alcohol preferably uses as a solution, and the solution preferably includes n-octyl alcohol and mine Object oil.
In the present invention, the volumetric concentration of n-octyl alcohol is preferably 0.1~10% in the solution, and more preferably 1%.
In the present invention, the anti-form-1,2- cyclohexanediol preferably use in form of an aqueous solutions.
In the present invention, anti-form-1 in the aqueous solution, the volumetric concentration of 2- cyclohexanediol are preferably 0.1~10%, more Preferably 10%.
In the present invention, the time lured preferably >=10min, more preferably 10~60min, most preferably 20~ 50min, further preferably 30~40min.
In the present invention, described to lure preferably at candlelight to carry out.
The present invention does not have special restriction to the type of the mineral oil, using commercially available quotient well known to those skilled in the art Product.
In the present invention, the diaphorina citri is preferably adult.
In the present invention, the application preferably includes following steps:
The alcohol is coated on mythimna separata Huang plate, then diaphorina citri is lured.
The present invention does not have special restriction to the concrete mode coated, using scheme well known to those skilled in the art ?.In the present invention, the diaphorina citri can be attracted on mythimna separata Huang plate, and the present invention can be attracted the diaphorina citri Not special restriction is operated after on to mythimna separata Huang plate, using mode of operation well known to those skilled in the art.
In another scheme of the invention, the application preferably includes following steps:
Shading treatment is carried out to the container for holding diaphorina citri;
The taste source bottle equipped with n-octyl alcohol is connected with rubber tube in the opening of the container;
Using the opening of the container as boundary, if diaphorina citri is crossed boundary and is considered as and is induced.
The present invention does not have special restriction to the concrete mode of the shading treatment, and use is well known to those skilled in the art Mode.
The present invention does not have special restriction to the size and material of the container.
The present invention does not have special restriction to the diameter and length of the rubber tube, n-octyl alcohol can be made to be volatized into container In.
In the present invention, it is also preferable to include mineral oil in the taste source bottle.
The present invention also provides a kind of diaphorina citri attractants, including alcohol and solvent, described when the alcohol is n-octyl alcohol Solvent is mineral oil, and when the alcohol is anti-form-1, when 2- cyclohexanediol, the solvent is water.
In the present invention, the volumetric concentration of alcohol is preferably 0.1~10% in the diaphorina citri attractant.
Application and a kind of citrus in diaphorina citri are being lured to n-octyl alcohol provided by the invention below with reference to embodiment Wood louse attractant is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
The solution for the n-octyl alcohol that volumetric concentration is 0%, 0.1%, 1%, 10% is respectively set, wherein 0% is blank control (as mineral oil) is separately connected with rubber tube consistent in length equipped with various concentration volatility at four-hole boiling flask different bottle mouth The taste source bottle of compound, is once put into 40 adults in four-hole boiling flask bottom, to cross the single port bottleneck of four-hole boiling flask as boundary, It crosses boundary and is considered as and be induced, the quantity and four bottleneck diaphorina citris of primary each bottleneck diaphorina citri were recorded every 10 minutes Total amount calculates induced efficiency.Original wood louse is all changed in experiment every time, in triplicate.Its result statistics is as shown in table 1, by Table 1 is as can be seen that 1% n-octyl alcohol solution has better epigamic to diaphorina citri.
The solution of the n-octyl alcohol of 1 different volumes concentration of table lures efficiency (%) to diaphorina citri
Embodiment 2
Compare the solution for the n-octyl alcohol that volumetric concentration is 1% with six arm olfactometers and mineral oil lures diaphorina citri Two walls in six arm olfactometers are placed the solution and mineral oil of the n-octyl alcohol that volumetric concentration is 1% by effect respectively, remaining four Arm is abandoned using closure arm mouth.Each arm air-flow velocity of six arm olfactometers is set as 80mL/min before experiment.Experiment test 10 every time Adult, tests 30 adults altogether, and when test carries out by head.Test worm is put into after olfactometer and observes and records its track of creeping, mandarin orange The number that tangerine wood louse enters preliminary examination selection area is denoted as the number that enters of area's tie substance, and diaphorina citri stops in final choice area The final choice number of area's tie substance is denoted as within 3 minutes when motionless.The results are shown in Table 2 for it, as can be seen from Table 2, this hair The method of bright offer lures efficiency with excellent.
The selection number and final dwell times of 2 diaphorina citri pair of table compare
Substance Into number Final dwell times
Mineral oil 2 2
1% n-octyl alcohol solution 6 3
Embodiment 3
(1) at four-hole boiling flask different bottle mouth with the rubber tube that length is 30cm be separately connected equipped with various concentration it is trans-- The taste source bottle of 1,2- cyclohexanediol solution;Anti-form-1,2- cyclohexanediol solution are prepared using ultrapure water, and volumetric concentration is respectively 0%, 0.1%, 1% and 10%, wherein 0% is blank control;
(2) shading treatment is carried out to the four-hole boiling flask for holding wood louse, is once put into about 40 adults in drag, with mistake The single port bottleneck of four-hole boiling flask is boundary, and wood louse, which crosses boundary and is considered as, to be induced, every the primary each bottleneck mandarin orange of 10 minutes records The total amount of the quantity of tangerine wood louse and four bottleneck diaphorina citris calculates induced efficiency.Original wood louse is all changed in experiment every time, In triplicate.
Its result statistics is as shown in table 3.According to table 3 as can be seen that concentration be 0.1~10% in the range of, anti-form-1,2- Cyclohexanediol has apparent inducing action, and the anti-form-1 that concentration is 10% to diaphorina citri, and 2- cyclohexanediol is to citrus The inductivity of wood louse is more preferable, and when induction time is 60min, its inductivity remains to reach 47.50% or so.
3 various concentration anti-form-1 of table, induced efficiency (%) of the 2- cyclohexanediol to diaphorina citri
Embodiment 4
(1) by 1st area of six arm olfactometers, 2nd area connection taste source bottle, remaining four area is vacant, wherein 2nd area are set as compareing Area;
(2) vacuum pump is opened, each arm air-flow velocity tune of six arm olfactometers is set as 80mL/min, and to its steady air current;
(3) the taste source corresponding to 1st area bottle in be put into 1% anti-form-1,2- cyclohexanediol solution (solvent is ultrapure water), 2 The ultrapure water that equivalent is put into the corresponding taste source bottle in area compares.
Experiment test every time is carried out by head, and record wood louse enters the number of different zones and stops 3 minutes motionless when institutes Number in region, as the test result that diaphorina citri reacts different volatile compound taxis, test result such as 4 institute of table Show.According to table 4 as can be seen that anti-form-1,2- cyclohexanediol have good attractant effect to diaphorina citri.
4 diaphorina citri of table compares anti-form-1, the selection number and final dwell times of 2- cyclohexanediol
Volatile matter Into number Final dwell times
Ultrapure water 1 1
1% anti-form-1,2- cyclohexanediol 8 7
Embodiment 5
In the dependent insect cage that specification is 75*75*90 (cm), (specification 15*20cm) carries out that worm is lured to test on yellow plate, Drug coated weight is 1mL or so (can increase in right amount) on every yellow plate.1% n-octyl alcohol is coated on yellow plate, with no drug Yellow plate is symmetrically hung in dependent insect cage, has been put into one plant of kamuning in centre in dependent insect cage, has been put into about 30 in dependent insect cage altogether Wood louse lures wood louse, and experimental result is as shown in table 5, and 1% n-octyl alcohol of the results show has attractant effect to wood louse.
In the dependent insect cage that specification is 75*75*90 (cm), (specification 15*20cm) carries out that worm is lured to test on yellow plate, Drug coated weight is 1mL or so (can increase in right amount) on every yellow plate.By 1% anti-form-1 of volumetric concentration, the coating of 2- cyclohexanediol It on yellow plate, is symmetrically hung in dependent insect cage with the yellow plate of no drug, has been put into one plant of kamuning in centre in dependent insect cage, supported About 30 wood louses are put into worm cage altogether, drug about 1mL was supplemented on yellow plate at second day, wood louse is lured, test The results are shown in Table 5, and 1% anti-form-1 of the results show, 2- cyclohexanediol has attractant effect to wood louse.
5 1% n-octyl alcohol of table and 10% anti-form-1,2- cyclohexenol lure worm result
Project First day Second day Third day
Control 3 4 4
N-octyl alcohol 8 10 13
Anti-form-1,2- cyclohexanediol 4 8 10
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (9)

1. alcohol is luring the application in diaphorina citri, the alcohol is n-octyl alcohol or anti-form-1,2- cyclohexanediol.
2. application according to claim 1, which is characterized in that the n-octyl alcohol uses as a solution, the solution Including n-octyl alcohol and mineral oil.
3. application according to claim 2, which is characterized in that in the solution volumetric concentration of n-octyl alcohol be 0.1~ 10%.
4. application according to claim 1, which is characterized in that the anti-form-1,2- cyclohexanediol is in form of an aqueous solutions It uses.
5. application according to claim 4, which is characterized in that anti-form-1 in the aqueous solution, the volume of 2- cyclohexanediol Concentration is 0.1~10%.
6. application according to claim 1, which is characterized in that the time lured is >=10min.
7. application according to claim 1, which is characterized in that it is described application the following steps are included:
The alcohol is coated on mythimna separata Huang plate, then diaphorina citri is lured.
8. a kind of diaphorina citri attractant, including alcohol and solvent, when the alcohol is n-octyl alcohol, the solvent is mineral oil, when The alcohol is anti-form-1, and when 2- cyclohexanediol, the solvent is water.
9. diaphorina citri attractant according to claim 8, which is characterized in that the body of alcohol in the diaphorina citri attractant Product concentration is 0.1~10%.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114304186A (en) * 2021-12-02 2022-04-12 岭南现代农业科学与技术广东省实验室 Mandarin orange psylla trapping agent and application thereof

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CN114304186A (en) * 2021-12-02 2022-04-12 岭南现代农业科学与技术广东省实验室 Mandarin orange psylla trapping agent and application thereof

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