CN117158439A - Attractant and lure for light buttock bark beetles, preparation method and application thereof - Google Patents
Attractant and lure for light buttock bark beetles, preparation method and application thereof Download PDFInfo
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- CN117158439A CN117158439A CN202311128317.9A CN202311128317A CN117158439A CN 117158439 A CN117158439 A CN 117158439A CN 202311128317 A CN202311128317 A CN 202311128317A CN 117158439 A CN117158439 A CN 117158439A
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- 241000545593 Scolytinae Species 0.000 title claims abstract description 76
- 239000005667 attractant Substances 0.000 title claims abstract description 58
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 57
- 210000001217 buttock Anatomy 0.000 title claims description 26
- 238000002360 preparation method Methods 0.000 title abstract description 9
- MBDOYVRWFFCFHM-SNAWJCMRSA-N (2E)-hexenal Chemical compound CCC\C=C\C=O MBDOYVRWFFCFHM-SNAWJCMRSA-N 0.000 claims abstract description 72
- HNVRRHSXBLFLIG-UHFFFAOYSA-N 3-hydroxy-3-methylbut-1-ene Chemical compound CC(C)(O)C=C HNVRRHSXBLFLIG-UHFFFAOYSA-N 0.000 claims abstract description 49
- MBDOYVRWFFCFHM-UHFFFAOYSA-N trans-2-hexenal Natural products CCCC=CC=O MBDOYVRWFFCFHM-UHFFFAOYSA-N 0.000 claims abstract description 36
- WONIGEXYPVIKFS-UHFFFAOYSA-N (+)-cis-Verbenol Natural products CC1=CC(O)C2C(C)(C)C1C2 WONIGEXYPVIKFS-UHFFFAOYSA-N 0.000 claims abstract description 27
- WONIGEXYPVIKFS-YIZRAAEISA-N (1s,2s,5s)-4,6,6-trimethylbicyclo[3.1.1]hept-3-en-2-ol Chemical compound CC1=C[C@H](O)[C@@H]2C(C)(C)[C@H]1C2 WONIGEXYPVIKFS-YIZRAAEISA-N 0.000 claims abstract description 27
- NFDFQCUYFHCNBW-SCGPFSFSSA-N dienestrol Chemical compound C=1C=C(O)C=CC=1\C(=C/C)\C(=C\C)\C1=CC=C(O)C=C1 NFDFQCUYFHCNBW-SCGPFSFSSA-N 0.000 claims abstract description 27
- 241000254173 Coleoptera Species 0.000 claims abstract description 26
- 229920003023 plastic Polymers 0.000 claims description 34
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- -1 polyethylene Polymers 0.000 claims description 32
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- 229920000573 polyethylene Polymers 0.000 claims description 31
- 229920000742 Cotton Polymers 0.000 claims description 23
- BZAZNULYLRVMSW-UHFFFAOYSA-N 2-Methyl-2-buten-3-ol Natural products CC(C)=C(C)O BZAZNULYLRVMSW-UHFFFAOYSA-N 0.000 claims description 17
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- 238000000034 method Methods 0.000 claims description 6
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- 238000004220 aggregation Methods 0.000 abstract description 18
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- 238000012360 testing method Methods 0.000 description 16
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000238414 Octopus vulgaris Species 0.000 description 2
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Abstract
The invention provides an attractant and a lure for light-hip-eight-tooth bark beetles, and a preparation method and application thereof, and belongs to the technical field of prevention and control of light-hip-eight-tooth bark beetles and green ecological prevention and control thereof. The attractant comprises the following components in parts by weight: 4 parts of 2-methyl-3-butene-2-ol, 0.04 part of (+ -) -bark beetle dienol, 0.02 part of (S) -cis-verbenol and 0.2 part of trans-2-hexenal. The attractant provided by the invention has excellent attracting effect on the light-hip-eight-tooth bark beetles, can increase the trapping amount by 3-4 times compared with the conventional light-hip-eight-tooth bark beetle aggregation pheromone attractant, has the advantages of strong specificity, long lasting period, environmental friendliness and the like, is suitable for long-term monitoring and high-efficiency control of the light-hip-eight-tooth bark beetle population, and is not influenced by topography and topography.
Description
Technical Field
The invention relates to an attractant and a lure for light-hip-eight-tooth bark beetles, and a preparation method and application thereof, and belongs to the technical fields of prevention and control of light-hip-eight-tooth bark beetles and green ecological prevention and control thereof.
Background
The genus Curvulonidae (Ips nitidus Eggers) is like Curvulonidae bark beetles Ips, is one of the main trunk-boring pests in the forest area of the western highland areas such as Qinghai province, and can harm the Qinghai spruce Picea crassifolia Kom, the Picea spruce P.purdurea Mast, the Tianshan spruce P.likiangensis var. Rubensens Rehder & E.H.Wilson and the like, and has the most serious harm to the specific tree species of Qinghai spruce and Picea spruce in Qinghai areas. The bark beetle belongs to a secondary pest and mainly damages weak spruces such as wind-back wood, wind-folded wood, weak wood (natural disasters and mechanical damaged wood), dying wood and the like in spruce forests; under the condition of high population density, healthy living woods can be directly affected, and the healthy living woods are often cooperated with Shangri-La bark beetle Ipsshangrila Cognato & Sun and eastern bark beetle Pseudips orientalis Wood & Yin, so that a great deal of woods die.
At present, the prevention and control of bark beetle type pests mainly depend on the trapping, killing, prevention and control of aggregation pheromones, and the prevention and control of bark beetle type pests are assisted to manually clean victims. In view of the similarity of the morphological characteristics and phylogenetic aspects of the ips Ips typographus Linnaeus and the light-hip-octopus, the ips which are distributed in the spruce forest of Qinghai province are the ips, so that the attempts of collecting pheromones and monitoring the adults of the light-hip-octopus are made by using the ips, but the 2 types of the ips are natural species which are mutually independent, so that the insect attracting effect is quite unsatisfactory. Later, the Qinghai province forestry sector also used known bark beetle aggregation pheromone components to screen potential bark beetle aggregation pheromone attractant in field trials in various combinations, without any substantial breakthrough. In 2009, zhang Qinghe et al identified the aggregation pheromone of the light buttock-octopus vulgaris internationally for the first time by adopting a male worm hindgut solvent extraction and chemical analysis means, and proposed an aggregation pheromone attractant component for attracting and killing the light buttock-octopus vulgaris, which is mainly: 65-97% of 2-methyl-3-butene-2-ol, 2-20% of (+/-) bark beetle dienol and 1-10% of (S) -cis-verbenol, which is helpful for long-term monitoring and efficient trapping, killing, prevention and control of the light buttock and eight-tooth bark beetle population.
In the process of invasion of the light buttock bark beetle to harm host plants, invasion behavior of the light buttock bark beetle is influenced by pheromone generated by the light buttock bark beetle, and volatile matters of plant sources are very important host positioning signal identification substances. The light-hip bark beetle recognizes and selects less resistant host trees for attack and ultimately successful colonization by taking advantage of the compositional differences of the volatile materials released by healthy and weakened trees. Therefore, the plant volatile matters can be used as behavior control substances of the bark beetles at the hip, and are used for increasing the trapping amount of the bark beetles or repelling the bark beetles. It has been found that some plant volatile matters have synergistic or inhibitory effect on the aggregation pheromone component of bark beetles, and the substances have the characteristics of wide sources, multiple types, environmental friendliness, low price and the like. Therefore, the behavior of the light-hip-eight-dentate bark beetle is regulated and controlled by utilizing volatile components from a host and a non-host, and plant volatile is added into an aggregation pheromone component as a synergistic agent or a compound agent, so that the insect attracting efficiency of the light-hip-eight-dentate bark beetle aggregation pheromone is improved, and the efficient ecological prevention and control function of the light-hip-eight-dentate bark beetle is realized.
Disclosure of Invention
Therefore, the invention aims to provide an attractant and a lure for the light buttock bark beetles, and a preparation method and application thereof. The attractant provided by the invention can obviously improve the insect attracting effect of the light-buttock and octopus dentatus aggregation pheromone, and realizes the efficient attracting of the light-buttock and octopus dentatus.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an attractant for light buttock bark beetles, which comprises the following components in parts by weight:
4 parts of 2-methyl-3-buten-2-ol;
(±) -bark beetle dienol, 0.04 weight portions;
(S) -cis-verbenol, 0.02 part;
0.2 part of trans-2-hexenal.
The invention also provides application of the attractant in attracting the light buttock-octopus type bark beetle.
The invention also provides a lure core for attracting the light buttock-octopus vulgaris, which comprises a slow-release carrier, absorbent cotton arranged in the slow-release carrier and the attractant adsorbed on the absorbent cotton.
Preferably, the slow release carrier is a polyethylene plastic bottle or a polyethylene plastic bag.
Preferably, in a single lure:
the content of 2-methyl-3-buten-2-ol was 4g;
the content of (+ -) -bark beetle dienol is 40mg;
the content of (S) -cis-verbenol was 20mg;
the content of trans-2-hexenal was 0.2g.
Preferably, the absorbent cotton content in the individual lures is 5g.
Preferably, the polyethylene plastic bottle has a capacity of 16ml and a wall thickness of 0.6mm; the polyethylene plastic bag has a thickness of 0.2mm, a length of 150mm and a width of 80mm.
The invention also provides a preparation method of the lure core, which comprises the following steps:
placing absorbent cotton into a polyethylene plastic bottle, dripping the attractant on the absorbent cotton, and finally filling into a polyethylene plastic bag for sealing to obtain the lure core.
The invention also provides application of the lure core in attracting light buttock-octopus type bark beetles.
Preferably, the application comprises: the lure is matched with a cross plate type bark beetle trapper for use, and the trapper is arranged at the edge of spruce or in open, sunny and ventilated areas in the forest, and the distance is 30-50 m.
The beneficial effects of the invention are as follows:
the attractant provided by the invention can obviously improve the insect attracting amount of the light-buttock and octopus dentatus aggregation pheromone, realizes efficient attracting of the light-buttock and octopus dentatus, has the advantages of strong specificity, excellent attracting effect, long lasting period, environmental friendliness and the like, is suitable for long-term monitoring and efficient control of the light-buttock and octopus dentatus population, and is not influenced by topography and topography when the attracting core is applied.
Drawings
FIG. 1 shows the results of the evaluation test of the biological activity of the 5-6 month light buttock-octopus amomum in 2022, wherein the results of the evaluation test of the biological activity of the example 1, the comparative example 2, the comparative example 3, the comparative example 4, the comparative example 5, the comparative example 6 and the comparative example 7 are shown in the sequence from left to right;
FIG. 2 shows the results of the biological activity evaluation test of the light-hip-eight bark beetles by the variation of the dosage of trans-2-hexenal in the attractant, which are shown in the following examples 2, 8, 9, 10 and 11 from left to right;
FIG. 3 shows the results of a long-term synergy evaluation test of the aggregation pheromone attractant for light-hip-octopus vulgaris by adding trans-2-hexenal (2023, 7, 23, 9, 11, huang Na, tibetan, mixiu forest farm, qinghai province), with line graphs of example 3, comparative example 12 and comparative example 13 from top to bottom.
Detailed Description
The invention provides an attractant for light buttock bark beetles, which comprises the following components in parts by weight:
4 parts of 2-methyl-3-buten-2-ol;
(±) -bark beetle dienol, 0.04 weight portions;
(S) -cis-verbenol, 0.02 part;
0.2 part of trans-2-hexenal.
The source of the above-mentioned reagent is not particularly limited, and a commercially available product may be used. In the present invention, the 2-methyl-3-buten-2-ol (microphone, purity > 98%), (±) -bark beetle dienol (carbofuran, purity > 95%), (S) -cis-verbenol (Sigma-Aldrich, purity > 95%), trans-2-hexenal (aladine, purity > 97%).
The invention also provides application of the attractant in attracting the light buttock-octopus type bark beetle.
The invention also provides a lure core for attracting the light buttock-octopus vulgaris, which comprises a slow-release carrier, absorbent cotton arranged in the slow-release carrier and the attractant adsorbed on the absorbent cotton. In the present invention, the slow release carrier is preferably a polyethylene plastic bottle or a polyethylene plastic bag.
In the present invention, the content of absorbent cotton in the individual lures is preferably 5g. In the invention, the capacity of the polyethylene plastic bottle is preferably 16ml, and the wall thickness of the bottle is 0.6mm; the polyethylene plastic bag is preferably a black polyethylene plastic bag with a thickness of 0.2mm, a length of 150mm and a width of 80mm. In the present invention, the content of 2-methyl-3-buten-2-ol in the individual lure is preferably 4g; the content of (+ -) -bark beetle dienol is preferably 40mg; the content of (S) -cis-verbenol is preferably 20mg; the content of trans-2-hexenal is preferably 0.2g. The invention has no special requirements on the sources of absorbent cotton, polyethylene plastic bottles and polyethylene plastic bags, and can be used by using commercial products.
The invention also provides a preparation method of the lure core, which comprises the following steps:
placing absorbent cotton into a polyethylene plastic bottle, dripping the attractant on the absorbent cotton, and finally filling into a polyethylene plastic bag for sealing to obtain the lure core.
In the present invention, the preparation method of the lure preferably comprises: dissolving said (+ -) -bark beetle dienol in the desired amount in 2-methyl-3-buten-2-ol, preferably at a concentration of 40mg/4g; the (S) -cis-verbenol is dissolved in 2-methyl-3-butene-2-ol in a required amount, and the concentration thereof is preferably 20mg/4g; the trans-2-hexenal is dissolved in the required amount in 2-methyl-3-buten-2-ol, preferably at a concentration of 0.2g/4g; the 4 components are evenly mixed and then added into absorbent cotton in a polyethylene plastic bottle carrier, and then the absorbent cotton is filled into a polyethylene plastic bag for sealing, so that the lure core is obtained.
The invention also provides application of the lure core in attracting light buttock-octopus type bark beetles.
The light buttock-octopus lure core provided by the invention can be used for trapping, killing, preventing and controlling light buttock-octopus, or utilizing the lure core to carry out long-term monitoring work of the light buttock-octopus population. The light buttock-octopus lure core provided by the invention is easy to process and produce, has low cost, and 4 components are respectively released stably on the carrier, the lasting period can be maintained for 2 months, and the requirement of attracting a large amount of light buttock-octopus lure on production can be met. The lure core provided by the invention is an efficient, lasting and environment-friendly lure core for light buttock and octopus vulgaris, is applied to the massive lure prevention and control of light buttock and octopus vulgaris, and has remarkable effect. The light buttock-octopus bark beetle lure core has wide industrialization prospect. In addition, the preparation method is simple, and is particularly suitable for large-area popularization in production.
In the invention, the lure core is used together with a cross plate type bark beetle trap for lure collection of light buttock octopus, and the trap is purchased from Fathoughty Sichuan Biotech Co. The big plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), the cross shape is connected with the upper cover through the hook at the top, and the cross shape is connected with the funnel through the hook at the bottom. The insect collecting bottle is directly connected with the bell mouth at the lower edge of the funnel. The lure is hung at the middle position of the trap, which is 20cm away from the top end. The trapper is fixed by 2 bamboo poles and iron wires, and is arranged at the edge of spruce forest or in open, sunny and ventilating zones, the distance is 30-50 m, and the height of the insect collecting bottle from the ground is about 50cm.
The technical scheme provided by the invention is described in detail below in connection with woodland biological activity tests, but the technical scheme is not meant to limit the scope of the invention.
Example 1
The high-efficiency attractant of the light buttock-octa bark beetle manufactured by the embodiment adopts commercially available 2-methyl-3-butene-2-ol, and the chemical purity and the isomeric purity are both more than 98 percent; (±) -bark beetle dienol, the chemical purity and the isomerism purity are both more than 95%; (S) -cis-verbenol, both chemical purity and isomeric purity of which are greater than 95%; the chemical purity and the isomerism purity of the trans-2-hexenal are both more than 97 percent.
High-efficiency attractant component:
4g of 2-methyl-3-buten-2-ol;
(±) -bark beetle dienol, 40mg;
(S) -cis-verbenol, 20mg;
trans-2-hexenal, 200mg.
The (+/-) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal are directly added into 2-methyl-3-butene-2-ol according to the proportion to prepare the attractant with different proportions.
And (3) preparing and preserving a lure: the attractant is added to 5g of absorbent cotton in a polyethylene plastic bottle carrier, and then the absorbent cotton is placed in a black polyethylene plastic bag for sealing.
The prepared lure core is sealed and packaged by aluminum foil paper and refrigerated at 4 ℃.
Comparative example 1
The attractant is as follows: 4g of 2-methyl-3-buten-2-ol, (±) -bark beetle dienol 40mg; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 2
The attractant is as follows: 4g of 2-methyl-3-buten-2-ol, (S) -cis-verbenol 20mg; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 3
The attractant is as follows: 4g of 2-methyl-3-buten-2-ol, 200mg of trans-2-hexenal; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 4
The attractant is as follows: 4g of 2-methyl-3-buten-2-ol, (±) -bark beetle dienol 40mg, and 200mg of trans-2-hexenal; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 5
The attractant is as follows: 4g of 2-methyl-3-buten-2-ol, (S) -cis-verbenol 20mg, trans-2-hexenal 200mg; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 6
The attractant is as follows: 4g of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol 40mg, (S) -cis-verbenol 20mg; the results are shown in FIG. 1, which is the same as in example 1.
Comparative example 7
No attractant and only a lure core.
Comparative examples 1 to 6 were prepared by directly adding (+ -) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal to 2-methyl-3-butene-2-ol in a certain amount. Comparative example 7 a trap (consisting of cotton wool, polyethylene plastic bottles and polyethylene plastic bags alone, without any components) was used as a blank.
The light buttock-octapin bark beetle attractant cores manufactured in the application example 1 and the comparison examples 1-7 are used for attracting the light buttock-octapin bark beetle, and the specific steps are as follows:
a) Manufacturing the trapper: the trap is mainly a cross-plate type bark beetle trap, purchased from the company of the middley Sichuan Biotech Co., ltd. The big plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), the cross shape is connected with the upper cover through the hook at the top, and the cross shape is connected with the funnel through the hook at the bottom. The horn mouth connected with the insect collecting bottle is sleeved at the lower part of the funnel and connected with the insect collecting bottle.
b) Suspension of lure: the middle position of the cross-plate type bark beetle trapper, which is 20cm away from the top end, is hung with 1 lure core.
c) Wild setting of the trapper: each test treatment was set up with 3 replicates, traps were set up at the spruce forest rim, and the trap spacing was about 30m. Two bamboo poles with the length of 2m and the diameter of 5cm are inserted into a soil layer for about 50cm, the distance between the two bamboo poles is 30cm, then the trap is fixed between the two bamboo poles, and the height of the trap insect collecting bottle is about 50cm away from the ground.
d) Data investigation and trap emptying: the test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. According to weather conditions, the number of bark beetles in the insect collecting bottle is observed every 2-3 days, and the bark beetles collected by the bait are placed in a plastic box to be brought to a laboratory after counting.
According to the specific application steps, the bait is used for attracting the light buttock octopus. The trap (comparative example 7) with the blank lure suspended was used as a blank control for 20 days 5-6-20 days 2022, and the specific results are shown in fig. 1. The distance between the traps is about 30m, and the distance between the insect collecting bottle and the ground is about 50cm. The test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. According to weather conditions, the number of bark beetles in the insect collecting bottle is observed every 2-3 days.
As can be seen from the analysis of the insect attracting effect in FIG. 1, the high-efficiency attractant for the light-hip-eight-dentate bark beetles (example 1) has the best trapping effect in example 1 compared with the different attractants (comparative examples 1, 2, 3, 4, 5 and 6), and the maximum insect attracting amount is more than 3 times that of the other attractant formulas. Comparative example 3 and comparative example 7 (blank control) had no attracting activity against light buttock octopus. The experiment further shows that the conventional aggregation pheromone attractant (comparative example 6) for the light-hip-octopus is capable of obviously enhancing the trapping efficiency of the light-hip-octopus after adding the trans-2-hexenal, can obviously improve the trapping amount by 3 times, and has a good attracting effect. The high-efficiency attractant for the light-hip-eight-tooth bark beetles prepared in the embodiment 1 can comprehensively prevent and control the light-hip-eight-tooth bark beetles in forests, and better protect spruce forests. The experimental conclusion confirms that the mixture of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal can induce and kill a large amount of light buttock octopus in woodland, wherein the trans-2-hexenal is a high-efficiency synergistic component for the conventional aggregation pheromone lure core of the light buttock octopus, and the mixture of 4 components forms the lure with strong lure effect on the light buttock octopus, which is indispensable.
Example 2
The high-efficiency attractant for the light buttock bark beetles manufactured by the embodiment comprises the following components:
4g of 2-methyl-3-buten-2-ol;
(±) -bark beetle dienol, 40mg;
(S) -cis-verbenol, 20mg;
trans-2-hexenal, 200mg.
The sample purity, the method for preparing the lure, the preservation of the lure and the implementation steps are the same as those of the example 1.
Comparative example 8
The attractant is as follows: 4g of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol 40mg, (S) -cis-verbenol 20mg; 20mg of trans-2-hexenal; the results are shown in FIG. 2, which is the same as in example 2.
Comparative example 9
The attractant is as follows: 4g of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol 40mg, (S) -cis-verbenol 20mg; 400mg of trans-2-hexenal; the results are shown in FIG. 2, which is the same as in example 2.
Comparative example 10
The attractant is as follows: 4g of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol 40mg, (S) -cis-verbenol 20mg; 2g of trans-2-hexenal; the results are shown in FIG. 2, which is the same as in example 2.
Comparative example 11
No attractant and only a lure core.
Comparative examples 8 to 10 were prepared by directly adding (+ -) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal to 2-methyl-3-butene-2-ol in a certain amount. Comparative example 11 a trap (consisting of sterile cotton wool, polyethylene plastic bottles and polyethylene plastic bags alone, without any added ingredients) was used as a blank for suspending the blank lure.
The light buttock octopus lure prepared in the example 2 and the comparative examples 8-11 is used for attracting the light buttock octopus lure, and the specific steps are as follows:
a) Manufacturing the trapper: the trap is mainly a cross-plate type bark beetle trap, purchased from the company of the middley Sichuan Biotech Co., ltd. The big plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), the cross shape is connected with the upper cover through the hook at the top, and the cross shape is connected with the funnel through the hook at the bottom. The horn mouth connected with the insect collecting bottle is sleeved at the lower part of the funnel and connected with the insect collecting bottle.
b) Suspension of lure: the middle position of the cross-plate type bark beetle trapper, which is 20cm away from the top end, is hung with 1 lure core.
c) Wild setting of the trapper: each test treatment was set up with 3 replicates, traps were set up at the spruce forest rim, and the trap spacing was about 30m. Two bamboo poles with the length of 2m and the diameter of 5cm are inserted into a soil layer for about 50cm, the distance between the two bamboo poles is 30cm, then the trap is fixed between the two bamboo poles, and the height of the trap insect collecting bottle is about 50cm away from the ground.
d) Data investigation and trap emptying: the test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. According to weather conditions, the number of bark beetles in the insect collecting bottle is observed every 2-3 days, and the bark beetles collected by the bait are placed in a plastic box to be brought to a laboratory after counting.
The biological activity test of the woodland of example 2 and comparative examples 8 to 11 was performed according to the above-described specific implementation procedure, with the light buttock-octopus amomum being performed on days 7, 30, 2022, and 30, 8, 30. The specific results are shown in FIG. 2. The test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. According to weather conditions, the number of bark beetles in the insect collecting bottle is observed every 2-3 days.
As can be seen from the analysis of the insect attracting effect in FIG. 2, the addition of trans-2-hexenal to the conventional aggregation pheromone attractant component of the P.light-hip bark beetle showed insect attracting activity at all of the tested doses of 20mg, 200mg, 400mg and 2g 4. The biological activity difference between the dosage of the trans-2-hexenal is not obvious between 200mg and 400mg, and the trapping amount is far greater than that at 20 mg. The trapping amount of trans-2-hexenal is highest at a dose of 200mg. Therefore, from the comprehensive consideration of the attracting effect, the lasting period and the economic use of the attractant attracting forests, 200mg of the trans-2-hexenal is selected as the optimal dosage in practical application.
Example 3
The high-efficiency attractant for the light buttock bark beetles manufactured by the embodiment comprises the following components:
4g of 2-methyl-3-buten-2-ol;
(±) -bark beetle dienol, 40mg;
(S) -cis-verbenol, 20mg;
trans-2-hexenal, 200mg.
The sample purity, the method for preparing the lure, the preservation of the lure and the implementation steps are the same as those of the example 1.
Comparative example 12
The attractant is as follows: 4g of 2-methyl-3-butene-2-ol, (±) -bark beetle dienol 40mg, (S) -cis-verbenol 20mg; the results are shown in FIG. 3, which is the same as in example 2.
Comparative example 13
No attractant and only a lure core.
Comparative example 12 was prepared by adding (+ -) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal directly to 2-methyl-3-buten-2-ol in set doses. Comparative example 13 a trap (consisting of cotton wool, polyethylene plastic bottles and polyethylene plastic bags alone, without any components added) was used as a blank for suspending a blank lure.
The light buttock-octapin bark beetle attractant cores manufactured in the application example 3 and the comparative examples 12-13 are used for attracting the light buttock-octapin bark beetle and comprise the following specific steps:
a) Manufacturing the trapper: the trap is mainly a cross-plate type bark beetle trap, purchased from the company of the middley Sichuan Biotech Co., ltd. The big plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), the cross shape is connected with the upper cover through the hook at the top, and the cross shape is connected with the funnel through the hook at the bottom. The horn mouth connected with the insect collecting bottle is sleeved at the lower part of the funnel and connected with the insect collecting bottle.
b) Suspension of lure: the middle position of the cross-plate type bark beetle trapper, which is 20cm away from the top end, is hung with 1 lure core.
c) Wild setting of the trapper: each test treatment was set up with 3 replicates, traps were set up at the spruce forest rim, and the trap spacing was about 30m. Two bamboo poles with the length of 2m and the diameter of 5cm are inserted into a soil layer for about 50cm, the distance between the two bamboo poles is 30cm, then the trap is fixed between the two bamboo poles, and the height of the trap insect collecting bottle is about 50cm away from the ground.
d) Data investigation and trap emptying: the test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. According to weather conditions, the number of bark beetles in the insect collecting bottle is observed every 5 days, and the bark beetles collected by the bait are placed in a plastic box to be brought to a laboratory after counting.
According to the above specific implementation steps, the woodland biological activity test of example 3 and comparative examples 12 to 13 was carried out on days 7, 23, 2023 and 11, and the trap (comparative example 13) with the hanging light buttocks bark beetle aggregation pheromone attractant (comparative example 12) was used as a positive control, and the trap with the hanging blank lure core was used as a blank control, and the specific results are shown in fig. 3. The test was set up with 3 replicates per treatment, and the number of bark beetles trapped per treatment was the average of 3 traps. The number of bark beetles in the insect collection bottle was observed every 5 days, depending on weather conditions.
From the analysis of the insect attracting effect in fig. 3, it can be seen that the conventional aggregation pheromone attractant (comparative example 12) for the light-hip-eight-dentate bark beetle has a good attracting effect in the whole monitoring investigation period (7 months 23 days to 9 months 11 days), and can basically and completely reflect the dynamic occurrence of the light-hip-eight-dentate bark beetle in the woodland. The light-hip-octopus high-efficiency attractant (example 3) has better trapping effect than that of comparative example 12. In the initial stage of the monitoring investigation (7 months 23 to 8 months 2 days), since the new generation of adults of the light buttock octopus is just eclosion, does not enter the flying stage and is affected by local rainfall climate, the trapping amount is less, but the trans-2-hexenal does not show remarkable synergy compared with comparative example 12. As the local weather becomes better, the air temperature rises, the light buttock octopus is in the flying period, the anti-2-hexenal has very good synergy on attracting the light buttock octopus, the monitoring sensitivity is obviously improved, the trapping amount is increased by 3-4 times compared with the comparative example 12, and the duration synergy time is about 50 days. The embodiment shows that the high-efficiency attractant for the light buttock-octopus prepared from 2-methyl-3-butene-2-ol, (+/-) -bark beetle dienol, (S) -cis-verbenol and trans-2-hexenal can remarkably improve the trapping amount of the light buttock-octopus, and the trans-2-hexenal has a long-term synergistic effect on the conventional aggregation pheromone component of the light buttock-octopus, so that the trans-2-hexenal can be used as the high-efficiency attracting synergistic agent to be applied to the trapping and killing of the light buttock-octopus, and the population dynamic change of the light buttock-octopus can be more effectively monitored.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (10)
1. The attractant for the light buttock bark beetles is characterized by comprising the following components in parts by weight:
4 parts of 2-methyl-3-butene-2-ol, 0.04 part of (+ -) -bark beetle dienol, 0.02 part of (S) -cis-verbenol and 0.2 part of trans-2-hexenal.
2. Use of the attractant of claim 1 for attracting light-hip-octopus.
3. A lure for attracting light-hip-octopus, comprising a slow-release carrier, absorbent cotton disposed in the slow-release carrier, and the attractant of claim 1 adsorbed on the absorbent cotton.
4. A lure according to claim 3, wherein the slow release carrier is a polyethylene plastic bottle or a polyethylene plastic bag.
5. A lure according to claim 3, wherein in a single lure:
the content of 2-methyl-3-buten-2-ol was 4g;
the content of (+ -) -bark beetle dienol is 40mg;
the content of (S) -cis-verbenol was 20mg;
the content of trans-2-hexenal was 0.2g.
6. A lure according to claim 3, wherein the mass of absorbent cotton in a single lure is 5g.
7. A lure according to claim 3, wherein the polyethylene plastic bottle has a capacity of 16ml and a wall thickness of 0.6mm; the polyethylene plastic bag has a thickness of 0.2mm, a length of 150mm and a width of 80mm.
8. A method of preparing a lure according to any one of claims 3 to 7, comprising the steps of:
placing absorbent cotton into a polyethylene plastic bottle, dripping the attractant on the absorbent cotton, and finally filling into a polyethylene plastic bag for sealing to obtain the lure core.
9. Use of a lure according to any one of claims 3 to 7 for attracting light-hip-octopus.
10. The application according to claim 9, characterized in that it comprises: the lure core is matched with a cross plate type bark beetle trapper, and is arranged at the edge of spruce or in open, sunny and ventilated areas in the forest, and the distance is 30-50 m.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311128317.9A CN117158439A (en) | 2023-09-04 | 2023-09-04 | Attractant and lure for light buttock bark beetles, preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311128317.9A CN117158439A (en) | 2023-09-04 | 2023-09-04 | Attractant and lure for light buttock bark beetles, preparation method and application thereof |
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| CN202311128317.9A Pending CN117158439A (en) | 2023-09-04 | 2023-09-04 | Attractant and lure for light buttock bark beetles, preparation method and application thereof |
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| CN (1) | CN117158439A (en) |
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