CN104839191B - A kind of for luring the compositions of Holotrichia parallela - Google Patents
A kind of for luring the compositions of Holotrichia parallela Download PDFInfo
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Abstract
The invention discloses a kind of Holotrichia parallela gyplure utilizing the trans 2 hexene ester potentiation of acetic acid, gyplure is (3 7) with the weight ratio of the trans 2 hexene esters of acetic acid: 1, gyplure includes Holotrichia parallela sex pheromone component and organic solvent, Holotrichia parallela sex pheromone component includes L Isoleucine methyl ester, (R) () linalool and 1 cyclohexane-carboxylic acid, its weight proportion is 6:1:0.1, and Holotrichia parallela sex pheromone component and organic solvent weight ratio are 1:3, described organic solvent is dichloromethane, one or more in methanol or acetone.Behavior Test shows that the EAG of female male worm 2 hexene ester proportioning groups trans to gyplure acetic acid and olfaction reaction value are preferable.The Holotrichia parallela field trapping test display trans 2 hexene esters of acetic acid to gyplure and gathering effect there is synergistic function, utilize plant volatiles and insect sex pheromone to monitor and prevent and treat Holotrichia parallela to provide foundation for collaborative.
Description
Technical field
The present invention relates to a kind of insect sex attractant utilizing botanical components potentiation, specifically, relate to a kind of for drawing
Lure the compositions of Holotrichia parallela.
Background technology
Holotrichia parallela (Holotrichia parallela) belongs to coleoptera (Coleoptera) scarab beetle Superfamily
(Scarabaeoidea) Melolonthidae (Melolonthidae), is that during agriculture forest and husbandry produces, the important soil of a class is dwelt insect.Its
The various crop such as larva Holotrichia diomphalia Bates harm Semen arachidis hypogaeae, Semen sojae atricolor, Radix Pachyrhizi Erosi, Semen Maydis, lawn, flowers, vegetable, often result in huge economic loss
(Hu Qiongbo, 2004；Liu Shu Lignum Rhamnellae, 2008).As a example by Semen arachidis hypogaeae, peanut grub generation quantity in all parts of the country in recent years is in year by year
Ascendant trend, causes serious harm to peanut production, and be typically injured underproduction 10-20%, severe patient 50-80%, even granule without
Receive (Li Xiao etc., 2012).Due to Holotrichia parallela for a long time at underground activities, therefore it being carried out prevention and control has certain difficulty,
Present stage is still based on chemical prevention.Additionally, pesticide residues, the problems such as environmental pollution exist, and make us in the urgent need to one
The novel approach planting green non-pollution carrys out pest control.
The use of insect sex pheromone in recent years provides a new approach for Pest control.Use insect sex pheromone
Carry out worm monitoring and the active height of non-environmental pollution control, high specificity, nontoxic, pollution-free, do not kill natural enemy, easy to use,
The advantage such as with low cost (Nehme et al., 2009；Nehme et al.,2010；Wang Guihua etc., 2013).Shandong Province's Semen arachidis hypogaeae
The sex pheromone of China's Holotrichia parallela studied by institute, independent research holotrichia parallela sex pheromone core and joining
Set trapper, and achieve in field good lure worm effect (Brockerhoff et al., 2006；Zhou et al.,
2009；Li Xiao etc., 2012).But there is problem (the Reddy and such as specific attractant male worm, consumption time be short in gyplure
Guerrero,2004；et al.,2005).In recent years, recognize chemically interactive between insecticide and plant along with people
Know and day by day deepen, utilize Chemical lab management principle, use the functional compounds regulations such as Secondary metabolites, control harmful organism
With protection target plant research obtained significant progress (Dickens et al., 1993；Dodds,2011；Yang Hui etc.,
2008；Yang Lang etc., 2004).Plant volatiles and insect pheromone are the important information materials of insecticide, the two synergism with
Behavior (Deng et al., the 2004a of regulation insecticide；Hanks and Millar,2012a；Jung et al.,2013).Logical
Cross increase tentaculum electric potential, pheromone receives neuron action potential and pulse frequency, and specialized plant source volatile matter can significantly increase
The allure of strong insect sex pheromone (Reddy and Guerrero, 2004；Wang Zhenhua etc., 2008).Research shows, Ji Zhuzhi
The secondary pollutant mass-energy regulation and control multiple behavior of plant-feed insect that thing self produces and discharges, such as orientation, mate and oviposit, takes food, escapes
Keep away, assemble, behavior (the Fettig et al., 2012, Lait et al., 2003 such as pollination；Pan et al.,2013；Wu Limin
Deng, 2012).But early stage is concentrated mainly on plant volatiles about the research of insect attractant prevents insect with insect pheromone
One-sided application in controlling (Negri and Bernik, 2008；Szendrei et al.,2011,Tooker et al.,
2002).Many document reports in recent years, botanical components is collaborative with insect pheromone plays regulating and controlling effect to insect lipids, it was demonstrated that
Plant volatiles has notable potentiation (Douglas et al .1993 to examination worm gyplure；Lu Yujie etc., 2003；Wang Gui
Flower etc., 2013).
It is the new technique of a kind of non-environmental pollution control insect with sex pheromone or gyplure pest control.Although it is existing both at home and abroad
Hundreds of insect sex attractants are applied, and Qu Mingjing has applied for one in the Chinese patent of Application No. 200910162405.4
Holotrichia parallela sex pheromone and gyplure, the most on this basis, by collaborative to botanical components and insect pheromone to insecticide
Behavior plays regulating and controlling effect, it is achieved examination worm gyplure has notable potentiation, is the technical problem to be solved in the present invention.
Summary of the invention
In order to solve problems of the prior art, it is an object of the invention to provide and Holotrichia parallela gyplure is had
A kind of plant lure composition of potentiation--acetic acid trans-2-hexene ester, acetic acid trans-2-hexene ester can cause female male worm relatively
Strong potentiometric response and olfaction reaction；Gyplure is deposited by Holotrichia parallela field trapping test display acetic acid trans-2-hexene ester
At synergistic function, utilize plant volatiles and insect sex pheromone to monitor and prevent and treat Holotrichia parallela to carry for collaborative
Supply foundation.
The technical scheme that the present invention takes is:
A kind of for luring the compositions of Holotrichia parallela, it is characterised in that gyplure and acetic acid trans-2-hexene ester
Weight ratio is (3-7): 1, and described gyplure includes Holotrichia parallela sex pheromone component and organic solvent, described Holotrichia parallela
Sex pheromone component include ILE methyl ester, (R)-(-)-linalool and 1-cyclohexane-carboxylic acid, its weight proportion is
6:1:0.1, and described Holotrichia parallela sex pheromone component and organic solvent weight ratio be 1:3, described organic solvent
For one or more in dichloromethane, methanol or acetone.
For luring the Electroantennogram response test method of the compositions of Holotrichia parallela, it is characterised in that specifically include
Following steps:
(1) preparation of stimulated samples
The preparation of plant volatiles single dose: each compound all with methanol as solvent, is configured to 0.1,1,10 μ g/ μ l 3
Concentration, compares with methanol；
The preparation of plant volatiles and gyplure mixture: the present invention by pheromone component ILE methyl ester, (R)-
(-)-linalool and 1-cyclohexane-carboxylic acid are that 6:1:0.1 ratio is hybridly prepared into Holotrichia parallela and lures according to weight proportion
Agent, with the mother solution of 1 μ g/ μ l, according to gyplure: the ratio mixing of plant volatiles=1:1,3:1,5:1,7:1,9:1, is joined
Mixture is used for indoor electric physiology and performance testing；
(2) feeler processes and EAG test program
Extract 10ul each chemical reagent sample with microsyringe, be added drop-wise to (25mm × 8mm) on doubling filter paper, wait to try
Putting in Pasteur pipe with tweezers by filter paper after agent volatilization 5min, ParafilmTM Pasteur pipe two ends, sample is at Guan Zhonghui
Measuring after sending out 5min, stimulating the phase of going through is 500ms, stimulus intervals about 30s, and the flow velocity of continuous flow is all with the flow velocity stimulating air-flow
4mL/s, every cephalont only takes 1 feeler, and each process 6 feelers of test, every feeler is repeated 3 times, and compares and process group
Measure alternately, with methanol as blank.
Further, described gyplure: the mixture of composition when acetic acid trans-2-hexene ester is 3:1, the insecticide of female worm touches
Angle electric potential signal (EAG) response value is 4.383 ± 0.13mV.
Further, described gyplure: the mixture of composition when acetic acid trans-2-hexene ester is 5:1, the insecticide of male worm touches
Angle electric potential signal (EAG) response value is 7.64 ± 0.59mV.
For luring the olfaction reaction method of testing of the compositions of Holotrichia parallela, it is characterised in that specifically include as follows
Step:
(1) preparation of stimulated samples
The preparation of plant volatiles single dose: each compound all with methanol as solvent, is configured to 0.1,1,10 μ g/ μ l3
Concentration, compares with methanol；
The preparation of plant volatiles and gyplure mixture: the present invention by pheromone component ILE methyl ester, (R)-
(-)-linalool and 1-cyclohexane-carboxylic acid are that 6:1:0.1 ratio is hybridly prepared into Holotrichia parallela and lures according to weight proportion
Agent, with the mother solution of 1 μ g/ μ l, according to gyplure: the ratio mixing of plant volatiles=1:1,3:1,5:1,7:1,9:1, is joined
Mixture is used for indoor electric physiology and performance testing；
(2) measuring the Holotrichia parallela sensitivity size to various reagent by Y-shaped olfactometer, olfactometer is placed in secretly
Room, room temperature 27 DEG C, air velocity 500mL/min, test takes each reagent dropwise of 10ul on filter paper bar, and put into Y-shaped pipe
At side arm air inlet, methanol compares, and often group exchanges test group and matched group seat after repeating, it is to avoid test error；Examination every time
The filter paper bar renewed after testing, every kind of reagent repeats for 3 times, and reagent of the same race changes Y-shaped pipe, used Y-shaped effective third after having surveyed
Ketone cleans, 60 DEG C of drying；The examination worm that often group test picking 10 was unearthed the same day and activity is strong, puts into Y-shaped pipe principal arm port,
Observe examination worm distribution situation after 10min, and calculate response rate, relative response rate, reaction coefficient carry out data analysis；
Response rate=[(borer population in borer population+control tube in taste source capsule)/test total borer population]；
Selective response rate=[borer population/(borer population in borer population+control tube in taste source capsule) in taste source capsule].
Further, described gyplure: the mixture of composition when acetic acid trans-2-hexene ester is 3:1 and 5:1, female worm
Selective response rate is the highest, respectively 0.93 ± 0.06 and 0.96 ± 0.06.
Further, described gyplure: the mixture of composition when acetic acid trans-2-hexene ester is 7:1, the selection of male worm is anti-
Should rate the highest, be 0.93 ± 0.03.
Acetic acid trans-2-hexene ester lures worm test method with the field of the mixture of Holotrichia parallela gyplure composition, and it is special
Levy and be, specifically include following steps: selecting field test base, every 60m arranges a trapper, and described trapper is by pushing up
Lid, funnel type device, intersection baffle plate and collection device composition, the baffle plate that wherein intersects is located at below top cover, and funnel device is located at friendship
Between fork baffle plate, collection device is located at below funnel device, and the suspension height of trapper (top) is 2.0m, with 360ug/ul's
Gyplure and acetic acid trans-2-hexene ester are configured to the lure of five different proportions of 1:1,3:1,5:1,7:1,9:1, institute by mother solution
Stating lure carrier and be mainly composed of absorbent cotton, concentration is 360mg/ lure, and lure is concentrated freezen protective at refrigerator, waved with plant source
Stimulating food matter single dose and gyplure as a control group, three repetitions of every lure every day, choose five unearthed day data be analyzed.Often
After it trapping terminates, recording each trapper and lure borer population amount, calculate the lure synergistic effect to gyplure, computational methods are as follows:
Further, described gyplure: during acetic acid trans-2-hexene ester=5:1, every lure lures female borer population to reach every day
Being 13.67 ± 0.67 to maximum, be significantly higher than 2.67 ± 0.33 of gyplure, property is lured by acetic acid trans-2-hexene ester
The synergistic effect of agent reaches 412%.
Further, described gyplure: during acetic acid trans-2-hexene ester=3:1, every lure lures male worm number to reach every day
Being 318.67 ± 10.84 to maximum, be significantly higher than 116 ± 2.65 of gyplure, property is lured by acetic acid trans-2-hexene ester
The synergistic effect of agent reaches 175%.
The invention have the benefit that
Attempt herein combining Holotrichia parallela gyplure with its host plant volatile matter, thus reduce into
This, improve the purposes such as pest trap rate.The host plant of Holotrichia parallela mainly has elm, mulberry etc., is planted by contrast host
The effective ingredient of thing leaf volatiles matter, picks the multiple frequently seen plants source from Holotrichia parallela host plant blade herein
Volatile matter, by Electroantennogram response (electroantennogram, EAG), and combines Y-shaped olfactometer and filters out the black dull gill
Gold Testudinis lures the potentiation of Holotrichia parallela than more sensitive chemical substance, test host plant volatile confrontation gyplure,
Result shows that acetic acid trans-2-hexene ester can cause the stronger potentiometric response of female male worm and olfaction reaction；By its with gyplure with
After different proportion mixing, Behavior Test shows that female male worm is preferable to EAG and the olfaction reaction value of mixture.Field trapping test shows
Show that acetic acid trans-2-hexene ester exists synergistic function to gyplure lure, work as gyplure: acetic acid trans-2-hexene ester=
During 5:1, every lure lures female borer population to reach maximum (13.67 ± 0.67) every day, reaches the synergistic effect of gyplure
412%；For male worm, when gyplure: during acetic acid trans-2-hexene ester=3:1, lure worm amount maximum every lure day
(318.67 ± 10.84), reach 175% to the synergistic effect of gyplure.Result herein utilizes Herbivore for collaborative
Material and insect sex pheromone monitor and prevent and treat Holotrichia parallela and provide foundation.
The compositions for luring Holotrichia parallela of present invention research and development, is either used for doing detecting and reporting pest information still with trapping
Method and Insect pest suppression method directly prevent and treat Holotrichia parallela insect pest, are all conducive to giving full play to the power of Biological control, in protection sky
Improve prevention effect while enemy, additionally avoid using chemical pesticide and can reduce pollution, protect environment, economic benefit, society's effect
Benefit and ecological benefits are notable.
Detailed description of the invention
The present invention is further illustrated below in conjunction with concrete experiment and interpretation of result.
1, materials and methods
(1) for examination insecticide and reagent
For examination insecticide: Holotrichia parallela picks up from Laixi, Qingdao of Shandong province Wangcheng Shandong Peanut Inst. proving ground,
After the Holotrichia parallela adult male and female adopted back are separated, raise with fresh leaf of elm tree in the incubator have passage respectively, cultivate
In case, soil moisture is maintained between 18%-20%.For examination insecticide Nature enemy 24 hours before the test.
For having a try agent: test agents useful for same is purchased from Sigma company, and each reagent purity is all more than 95%.Concrete reagent is shown in
Table 1.
Behavior reaction test instrunment: (1) tentaculum electric potential instrument by fine motion manipulator INR-5, data acquisition controller IDAC-4,
Odor stimulation controls device cs-05 and tentaculum electric potential record display output device composition, originates from Syntech company of Germany.(2) smell
Feel that instrument consists of the following components: ACO-006 type electromagnetic type air compressor (production of Sheng Group Co., Ltd of Guangdong Province), work
Property charcoal air cleaner and LZB-4 type glass rotameter (Changzhou Shuanghuan Thermo-Technical Instrument Co., Ltd.'s production), 250ml special
Family name's porous filter flask (built-in water), tee T, Y-shaped smell sensing apparatus (two brachium 12cm, the long 20cm of base tube, two-arm angle 45 °, caliber
3.5cm)。
Lure and trapper: the lure and the supporting trapper thereof that use in test are carried by Shandong Semen arachidis hypogaeae Research Institute
Supply.Lure carrier is mainly composed of absorbent cotton, and concentration is 360mg/ lure.Trapper is by top cover, funnel type device, intersection baffle plate
Forming with collection device, the baffle plate that wherein intersects is located at below top cover, and funnel device is located between intersection baffle plate, and collection device is located at
Below funnel device, there is strong volatile lure and concentrate freezen protective at refrigerator.
More than a kind of host plant volatile matter of table
(2) test method
1) preparation of stimulated samples
The preparation of plant volatiles single dose: each compound all with methanol as solvent, is configured to 0.1,1,10 μ g/ μ l 3
Concentration, compares with methanol.
The preparation of plant volatiles and gyplure mixture: according to Zhou etc. by Isoleucine methyl ester and linalool with 6:1
Ratio is hybridly prepared into Holotrichia parallela gyplure.With the mother solution of 1 μ g/ μ l, according to gyplure: plant volatiles=1:1,3:
1, the ratio mixing of 5:1,7:1,9:1, be compounded agent is used for indoor electric physiology and performance testing.
2) feeler processes and EAG test program
With reference to Holotrichia parallela feeler processing methods (Zhou et al., 2009) such as zhou.Extract with microsyringe
10ul each chemical reagent sample, is added drop-wise to (25mm × 8mm) on doubling filter paper, with tweezers by filter paper after candidate agent volatilization 5min
Sheet is put in Pasteur pipe, ParafilmTM Pasteur pipe two ends, and sample volatilizees in pipe and measures after 5min, stimulates the phase of going through to be
500ms, stimulus intervals about 30s.The flow velocity of continuous flow is all 4mL/s with the flow velocity stimulating air-flow, and every cephalont is only taken 1 and touches
Angle, each process 6 feelers of test, every feeler is repeated 3 times, and compares the mensuration with process group alternately, with methanol as sky
White comparison.
3) olfaction reaction test
This patent measures the Holotrichia parallela sensitivity size to various reagent by Y-shaped olfactometer.Olfactometer is placed in
Darkroom, room temperature 27 DEG C, air velocity 500mL/min.Test takes each reagent dropwise of 10ul on filter paper bar, and put into Y-shaped
At tube side arm air inlet, methanol compares, and often group exchanges test group and matched group seat after repeating, it is to avoid test error.Every time
The filter paper bar renewed after test, every kind of reagent repeats for 3 times, and reagent of the same race changes Y-shaped pipe after having surveyed, and used Y-shaped is effective
Acetone cleans, 60 DEG C of drying.The examination worm that often group test picking 10 was unearthed the same day and activity is strong, puts into Y-shaped pipe principal arm port,
Observe examination worm distribution situation after 10min, and calculate response rate, relative response rate, reaction coefficient carry out data analysis.
Response rate=[(borer population in borer population+control tube in taste source capsule)/test total borer population]；
Selective response rate=[borer population/(borer population in borer population+control tube in taste source capsule) in taste source capsule].
4) plant volatiles lures worm to test with the field of gyplure mixture
Field test is carried out in proving ground, Wangcheng, Shandong Peanut Inst. Laixi.Every 60m arranges a trapper, lures
The suspension height catching device (top) is 2.0m.With the mother solution (Li Xiao etc., 2012) of 360ug/ul by gyplure and the trans-2-of acetic acid
Hexene ester is configured to the lure of five different proportions of 1:1,3:1,5:1,7:1,9:1, lures with plant volatiles matter single dose and property
Agent as a control group, three repetitions of every lure every day, choose five unearthed day data be analyzed.After trapping every day terminates, note
Record each trapper and lure borer population amount, calculate the lure synergistic effect to gyplure.Computational methods are as follows:
1.5 data process
Using spss 16.0 software that data carry out computational analysis, multiple comparisons uses DuncanShi duncan's new multiple range method.
2, result
(1) Holotrichia parallela is to the electro physiology of various plants source volatile matter and behavior reaction
1) impact that Holotrichia parallela EAG is reacted by plant volatiles
The female male worm of Holotrichia parallela is different (table 2) to the EAG response value of reagent variable concentrations of the same race.Female male worm is all to acetic acid
Trans-2-hexene ester shows the EAG response value being significantly higher than other reagent, the EAG value of female worm-2-trans to acetic acid hexene ester
Being 5.14 ± 0.25mV, male worm is 5.68 ± 0.41mV.
The EAG of host plant volatile matter is reacted by table 2 Holotrichia parallela
In table, data are average ± standard error.Examine through DuncanShi duncan's new multiple range method with letter representations different after column data
Test at P < 0.05 level difference notable.
* in table, numerical value is the EAG value using methanol as CK.
2) plant volatiles impact on Holotrichia parallela olfaction reaction
As can be seen from Table 2, the response rate of selected examination worm is all more than 80%, and is not significantly different between each process, says
Bright examination worm is healthy, this research experiment reliable results.From selective response rate it can be seen that Holotrichia parallela is dense to reagent difference of the same race
The response value of degree is different.The selective response rate of female worm-2-trans to acetic acid hexene ester is the highest, is 0.92 ± 0.04, is significantly higher than
Other reagent of variable concentrations.For male worm, the diversity between each reagent is more significant, and wherein male worm-2-trans to acetic acid is own
The olfaction reaction value of alkene ester is significantly higher than other reagent, is 0.96 ± 0.04.
Table 3 Holotrichia parallela olfaction reaction * to host plant volatile matter
In table, data are average ± standard error.Examine through DuncanShi duncan's new multiple range method with letter representations different after column data
Test at P < 0.05 level difference notable.
* in table, numerical value is the olfaction reaction value using methanol as CK.
(2) synergism test of host plant volatile confrontation gyplure
From 2.1, EAG response value and the olfaction reaction value of male and female Holotrichia parallela-2-trans to acetic acid hexene ester are equal
Higher than other reagent, and male and female Holotrichia parallela has higher sensitivity, therefore this patent choosing to the reagent of 1ug/ul concentration
Take acetic acid trans-2-hexene ester, (the most mixed with the mixture that gyplure is configured to 1ug/ul by different proportion (m:m) respectively
Agent), carry out potentiation test.
1) mixture impact on Holotrichia parallela Electroantennogram response
The female male worm of Holotrichia parallela is different (table 4) to the EAG response value of different ratio group.Work as gyplure: the trans-2-of acetic acid
When hexene ester is 3:1, female worm EAG response value is significantly higher than other proportioning group, is 4.383 ± 0.13mV.The property of 5:1 is lured by male worm
Agent: the EAG response value of acetic acid trans-2-hexene ester mixture is significantly higher than other proportioning groups, is 7.64 ± 0.59mV.
The EAG of gyplure with host plant volatile matter mix reagent is reacted by table 4 Holotrichia parallela
In table, data are average ± standard error.Examine through DuncanShi duncan's new multiple range method with letter representations different after column data
Test at P < 0.05 level difference notable.
* in table, numerical value is the EAG value using gyplure as CK.
2) mixture impact on Holotrichia parallela olfaction reaction
As can be seen from Table 5, the response rate of selected examination worm is all more than 80%, and does not has significant difference between each process,
Illustrate to try worm healthy, this research experiment reliable results.From selective response rate it can be seen that Holotrichia parallela is to different ratio group
Olfaction reaction value is different.When gyplure: when acetic acid trans-2-hexene ester is 3:1 and 5:1, the olfaction reaction value of female worm is the highest, point
It is not 0.93 ± 0.06 and 0.96 ± 0.06, is significantly higher than other proportioning groups.Work as gyplure: acetic acid trans-2-hexene ester is 7:1
Time, the olfaction reaction value of male worm is significantly higher than other proportioning groups, is 0.93 ± 0.03.
Table 5 Holotrichia parallela olfaction reaction * to gyplure Yu host plant volatile matter mix reagent
In table, data are average ± standard error.Examine through DuncanShi duncan's new multiple range method with letter representations different after column data
Test at P < 0.05 level difference notable.
* in table, numerical value is the olfaction reaction value using gyplure as CK.
(3) field adult trap effect is analyzed
Comprehensive 2.2EAG reaction and olfaction reaction result, male and female Holotrichia parallela-2-trans to acetic acid hexene ester respectively mixes
Reagent sensitivity is above other combos, is therefore mixed in various proportions by trans to gyplure and acetic acid-2-hexene ester, carries out field
Between test.
As shown in Table 6, the quantity of each mixture trapping Holotrichia parallela adult is all remarkably higher than acetic acid trans-2-hexene ester list
Agent or gyplure.When gyplure: during acetic acid trans-2-hexene ester=5:1, every lure lures female borer population to reach maximum every day,
It is 13.67 ± 0.67, is significantly higher than 2.67 ± 0.33 of gyplure, the potentiation to gyplure of the acetic acid trans-2-hexene ester
Effect reaches 412%.For male worm, when gyplure: during acetic acid trans-2-hexene ester=3:1, lure worm amount the most every lure day
Greatly, being 318.67 ± 10.84, be significantly higher than 116 ± 2.65 of gyplure, acetic acid trans-2-hexene ester is to gyplure
Synergistic effect reaches 175%.
The field trap effect of table 6 Holotrichia parallela gyplure and plant volatiles mixture is analyzed
In table, data are average ± standard error.Examine through DuncanShi duncan's new multiple range method with letter representations different after column data
Test at P < 0.05 level difference notable.
The above is not limitation of the present invention, it should be pointed out that: those skilled in the art are come
Saying, on the premise of without departing from essential scope of the present invention, it is also possible to make some changes, retrofit, add or replace, these improve
Also protection scope of the present invention is should be regarded as with retouching.
Claims (8)
1. the compositions being used for luring Holotrichia parallela, it is characterised in that gyplure and the weight of acetic acid trans-2-hexene ester
Amount ratio is (3-7): 1, and described gyplure includes Holotrichia parallela sex pheromone component and organic solvent, described Holotrichia parallela
Pheromone component include ILE methyl ester, (R)-(-)-linalool and 1-cyclohexane-carboxylic acid, its weight proportion is 6:
1:0.1, and described Holotrichia parallela sex pheromone component and organic solvent weight ratio be 1:3, and described organic solvent is
One or more in dichloromethane, methanol or acetone.
The most according to claim 1 for luring the compositions of Holotrichia parallela, it is characterised in that described compositions is used for drawing
Lure the purposes of Holotrichia parallela.
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, its
It is characterised by, described gyplure: the mixture of composition when acetic acid trans-2-hexene ester is 3:1, the Electroantennogram letter of female worm
Number (EAG) response value is 4.383 ± 0.13mV.
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, institute
The gyplure stated: the mixture of composition when acetic acid trans-2-hexene ester is 5:1, Electroantennogram signal (EAG) reaction of male worm
Value is 7.64 ± 0.59mV.
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, institute
The gyplure stated: the mixture of composition when acetic acid trans-2-hexene ester is 3:1 and 5:1, the selective response rate of female worm is the highest, respectively
It is 0.93 ± 0.06 and 0.96 ± 0.06.
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, institute
The gyplure stated: the mixture of composition, the olfactory sensation selective response rate of male worm when acetic acid trans-2-hexene ester is 7:1 are 0.93 ±
0.03。
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, institute
The gyplure stated: during acetic acid trans-2-hexene ester=5:1, every lure lure every day female borer population reach maximum be 13.67 ±
0.67, being significantly higher than 2.67 ± 0.33 of gyplure, the synergistic effect of gyplure is reached by acetic acid trans-2-hexene ester
412%.
The most according to claim 2 for lure the compositions of Holotrichia parallela for luring the purposes of Holotrichia parallela, institute
The gyplure stated: during acetic acid trans-2-hexene ester=3:1, every lure lure every day male worm number reach maximum be 318.67 ±
10.84, being significantly higher than 116 ± 2.65 of gyplure, the synergistic effect of gyplure is reached by acetic acid trans-2-hexene ester
175%.
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| CN108552207A (en) * | 2018-05-21 | 2018-09-21 | 山东省花生研究所 | A kind of trans- Synergistic slow release gelling agent of Holotrichia parallela first and its preparation and effect |
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| CN105052930B (en) * | 2015-08-21 | 2017-11-17 | 中国农业科学院植物保护研究所 | A kind of Holotrichia parallela lure composition and its application |
| CN105410038B (en) * | 2015-11-17 | 2018-04-10 | 山东省花生研究所 | A kind of Holotrichia parallela sex pheromone synergist and preparation method |
| CN105341003B (en) * | 2015-11-25 | 2018-04-10 | 山东省花生研究所 | The Holotrichia parallela gyplure preparation method that a kind of rainy day uses |
| CN108684671A (en) * | 2018-05-21 | 2018-10-23 | 山东省花生研究所 | A kind of cis- Synergistic slow release gelling agent of Holotrichia parallela first and its preparation and effect |
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| CN101627757B (en) * | 2009-08-03 | 2012-08-22 | 山东省花生研究所 | Dark melolonthidae sex pheromone and sex attractant |
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Effective date of registration: 20221208 Address after: Room 211, Block D, Lanhua Institute, No. 36, Jinshui Road, Laoshan District, Qingdao City, Shandong Province, 266000 Patentee after: QINGDAO RUSSELL IPM BIOTECH CO.,LTD. Address before: 266000 No. 126 floating Hill Road, Licang District, Shandong, Qingdao Patentee before: SHANDONG PEANUT Research Institute |