CN102318590A - A kind of method of trapping mymaridae parasitized empoasca vitis - Google Patents
A kind of method of trapping mymaridae parasitized empoasca vitis Download PDFInfo
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- CN102318590A CN102318590A CN201110160807A CN201110160807A CN102318590A CN 102318590 A CN102318590 A CN 102318590A CN 201110160807 A CN201110160807 A CN 201110160807A CN 201110160807 A CN201110160807 A CN 201110160807A CN 102318590 A CN102318590 A CN 102318590A
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- leafhopper
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000351086 Mymaridae Species 0.000 title claims abstract description 15
- 241000086608 Empoasca vitis Species 0.000 title claims abstract description 13
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- 241000317929 Chalcididae Species 0.000 claims abstract description 80
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- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 17
- 210000004681 ovum Anatomy 0.000 claims abstract description 17
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Abstract
The present invention provides a kind of method of trapping mymaridae parasitized empoasca vitis, with α-farnesene, cis- jasmone, 4 kinds of synomones of gaultherolin and benzaldehyde inducing agent is made into as taste source, the leaf tea tip of 1 bud 2 is copied with rubber, immersion inducing agent is used as lure, latter stage is contained in leafhopper population beginning peak period, leafhopper population peak period and leafhopper population, lure, trapping mymaridae parasitized empoasca vitis ovum are placed in tea place.The present invention prepares inducing agent from the strong synomone of activity, traps the tassel chalcid fly restriction leafhopper in the natural environment of tea place, reduces or remits chemical prevention.The inventive method is environmentally friendly, nuisanceless, meets national green prevention principle, and is conducive to protecting and utilizing nature enemy in amount, can promote the development of organic tea industry, meets construction requirements of the Ministry of Agriculture on standard Ecological Tea Gardens.Method is easy to operate, instant effect, low cost, suitable for popularization and application.
Description
Technical field
The invention belongs to tea plant pest prevention and controls, it is related to a kind of method of trapping mymaridae parasitized empoasca vitis, more particularly to a kind of method that use tea tree synomone trapped and guided the rod tassel chalcid fly of leafhopper three and the parasitic important pests false eye leafhopper ovum of small rift valley tassel chalcid fly.It is related to the synomone that separation identification leafhopper lancet thorn inhales the tea tip tender stem release that cause harm the tea tip, leafhopper tube for laying eggs are stabbed, determines the component for wherein having notable allure to tassel chalcid fly, tassel chalcid fly inducing agent is made；Tassel chalcid fly inducing agent in the leaf tea tip of 1 bud 2, load is copied with rubber, lure is made；In false eye leafhopper beginning peak period, peak period and Sheng latter stage, trapped using lure, guide two kinds of tassel chalcid flies searches, parasitic false eye leafhopper.
Background technology
In decades, false eye leafhopperEmpoasca vitis GThe is just seriously caused harm in the national tea area of the neighbour such as China mainland tea place and Vietnam, Burma.The insect is more from generation to generation, reproductive capacity is strong, lacks effective natural enemies, relies primarily on chemical prevention, has caused it to produce the very strong resistance to the action of a drug.Speculate that the pesticide volume for preventing and treating the leafhopper every year accounts for the 40% of the total formulation rate in tea place in the industry.Since the nineties, China widelys popularize in organic tea, this kind of tea place and disables chemical pesticide, and the leafhopper is just into the bottleneck of limitation organic tea production.
However, this seminar finds the rod tassel chalcid fly of leafhopper three in Zhejiang, Soviet Union, Anhui Cha Qu in recent yearsStethynium empoascaeSubba Rao and small fractured bone tassel chalcid flySchizophragma parvulaOgloblin is higher for the parasitic rate of the leafhopper ovum.The tea place of 10 types is selected in Hangzhou West Lake Dragon Well tea area, 1 Ci Ding gardens investigation in 5 days finds that two kinds of tassel chalcid flies are stronger to the following effect of the leafhopper.In Fujian Province Kingsoft, Fuan Cha Qu, also higher, following effect is also stronger for parasitic rate of both honeybees to the leafhopper（Lin Nai selects seminar of University Of Agriculture and Forestry In Fujian reports）.The scientific name of both honeybees is all professor's Lin Naiquan identification, the raising for not having both honeybees so far, the report bred；And under field conditions (factors), the Natural Population of both honeybees is not enough to control the leafhopper.The tassel chalcid fly of Natural Survival in the group of tea place is trapped, is directed in highdensity false eye leafhopper population in the inducing agent of tassel chalcid fly, short time if development can be trapped effectively, searching efficiency certainly will be strengthened, parasitic rate is improved, leafhopper population density is effectively forced down.
The research of chemical communication mechanism is confirmed between plant-insect-natural enemy three-level nutrition since the nineties, and insect pest plant discharges volatility synomone as distress signal, lures natural enemy to come parasitic or prey on insect；In recent years several document reports are had：Synomone formula is filtered out, the inducing agent of preparation effectively can trap natural enemy and improve parasitic rate of the parasitic wasp for insect.Color, body form in also substantial amounts of document report habitat also have seduction to insect.This seminar tentatively finds, the tea tip tender stem smell that tea tip smell that both tassel chalcid fly preference leafhopper lancet thorns are drawn through, the leafhopper oopod are stabbed.Intend the component that tassel chalcid fly is lured in the separation identification from these smells, prepare the significant inducing agent of gathering effect, be loaded in suitable lure, discharged in the proper control time of leafhopper, trapping mymaridae parasitized leafhopper.
False eye leafhopper typically forms 2 insect population peaks in mid-May-late July, the 9-11 months, and quantity is larger in the fall for two kinds of tassel chalcid flies.Intend discharging inducing agent in highdensity leafhopper population in leafhopper population beginning mid-September peak period, early October leafhopper population peak period, the search of tassel chalcid fly, parasitic leafhopper ovum are trapped, to suppress leafhopper population quantity；Early November is that leafhopper population contains latter stage, and the hibernating egg of tassel chalcid fly ovipositing hosts leafhopper is trapped with inducing agent, next year insect density is forced down, and mitigates it and spring tea, summer tea are caused harm, the usage amount of chemical pesticide is reduced or remitted.
The content of the invention
It is an object of the present invention to provide a kind of method of trapping mymaridae parasitized empoasca vitis, it is a kind of method for trapping the rod tassel chalcid fly of leafhopper three and the parasitic false eye leafhopper ovum of small fractured bone tassel chalcid fly in tea place, is realized by following steps：
（1）From the strong α-farnesene of activity, cis- jasmone, 4 kinds of synomones of gaultherolin and benzaldehyde as taste source, every kind of taste source concentration is all 10-2G/ml, using n-hexane as solvent, by 1: 0.1 15:0.1
15:0.1
15 ratio is made into tassel chalcid fly and traps agent solution；
（2）The 1 bud 2 leaf tea tip is copied with rubber, the is uploaded（1）The inducing agent of step, is made tassel chalcid fly lure；Tea tip immersion tassel chalcid fly is specifically trapped into agent solution（Concentration is 10-2 g/ml）12 
36 hours, taking-up was dried, and tassel chalcid fly lure is made, and sealing, low tempertaure storage is standby；
（3）Latter stage is contained in leafhopper population beginning peak period, leafhopper population peak period and leafhopper population, lure, trapping mymaridae parasitized empoasca vitis ovum are placed in tea place.Lure is hung on tea clump upper strata.
There are 2 insect population peaks in mid-May-late July, the 9-11 months every year in false eye leafhopper, the quantity of two kinds of tassel chalcid flies of August starts increase, in leafhopper population beginning mid-September peak period and early October leafhopper population peak period, place lure, trap the tassel chalcid fly in the non-tea place habitat of in tea place and tea place surrounding, its search, parasitic leafhopper ovum are guided, to suppress leafhopper population density.Early November leafhopper population is contained latter stage, and placement lure traps tassel chalcid fly and laid eggs in leafhopper ovum, crosses winter, forces down leafhopper hibernating base, to reduce spring in next year, summer insect density, and mitigates and spring tea, summer tea are caused harm.
It is a further object to provide a kind of application of method of trapping mymaridae parasitized empoasca vitis in the rod tassel chalcid fly of leafhopper in trapping tea place three and the parasitic false eye leafhopper ovum of small fractured bone tassel chalcid fly.
The present invention separates synomone during the smell for inhaling the tea tip, the tea tip tender stem smell that spawning is stabbed are pierced from leafhopper, detect its attractive activity for tassel chalcid fly, inducing agent is prepared from the strong synomone of several activity, when tassel chalcid fly density is larger in the fall, the tassel chalcid fly restriction leafhopper in the natural environment of tea place is trapped, chemical prevention is reduced or remitted.The inventive method is environmentally friendly, nuisanceless, meets national green prevention principle, and is conducive to protecting and utilizing nature enemy in amount, can promote the development of organic tea industry, meets construction requirements of the Ministry of Agriculture on standard Ecological Tea Gardens.Method is easy to operate, instant effect, low cost, suitable for popularization and application.
Brief description of the drawings
Fig. 1 is tassel chalcid fly lure structural representation.
Fig. 2 is healthy tea tree plant volatiles total ion chromatogram.
Fig. 3 is that leafhopper thorn suction is caused harm tea tree plant volatiles total ion chromatogram.
Fig. 4 is healthy tender stem volatile matter total ion chromatogram.
Fig. 5 is that tender stem volatile matter total ion chromatogram is stabbed in spawning.
Fig. 6 is Four-armed olfactomete measuring principle schematic diagram.
Fig. 7 is that 7 kinds of taste sources are compareed with it（Atoleine）It is detained time and its difference of the rod tassel chalcid fly of leafhopper three.
Fig. 8 is that 7 kinds of taste sources are compareed with it（Atoleine）It is detained time and its difference of small fractured bone tassel chalcid fly.
Fig. 9 is the tassel chalcid fly quantity and difference of 8 kinds of lure captures.
Figure 10 is averagely every piece colour table a face（Attached lure）B faces（Not attached lure）The tassel chalcid fly number of trapping（Colour table quantity=80）.
Embodiment
The present invention is further described in conjunction with the accompanying drawings and embodiments.
1st step separation identification health and cicada do harm to tea plant volatiles and health and tea tree tender stem volatile matter is stabbed in spawning
Ground part, healthy tender stem, oopod that the ground part of potted plant healthy tea tree plant, leafhopper thorn inhale tea tree plant of causing harm are stabbed tender stem and be put into cleaned glass cover respectively, cleaned air is passed through from air inlet, entrainment tea tree smell flows through the Super Q adsorption columns for being connected to gas outlet, Dynamic Adsorption 810 hours, then adsorption column is eluted with chromatographic pure dichloromethane, eluent is slowly concentrated into 20 μ l or so under high pure nitrogen stream.Draw 1 μ l analyzed through gas chromatograph-mass spectrometer, matching standard sample it is qualitative, add internal standard ethyl caprate and quantify, measure healthy tea plant volatiles（Fig. 2）Tea plant volatiles are done harm to cicada（Fig. 3）Ion stream chromatogram, and qualitative and quantitative analysis each component（Table 1）.Measure healthy tender stem volatile matter（Fig. 4）, spawning stab tender stem volatile matter（Fig. 5）Ion stream chromatogram, qualitative and quantitative analysis each component（Table 2）.In Fig. 2：1 is trans- 2- hexenoic aldehydes, and 2 be cis- blatter alcohol, and 3 be cis- 3- hexenyl acetates, and 4 be 2-Ethylhexyl Alcohol, and 5 are
- ocimenum, 6 be linalool, and 7 be nonyl alcohol, and 8 be cis- butyric acid-hexene ester, and 9 be capraldehyde, and 10 be the tetradecane, and 11 be carypohyllene, and 12 be geraniol, and 13 be hexadecane.In Fig. 3：1 is hexanal, and 2 be trans- 2- hexenoic aldehydes, and 3 be cis- blatter alcohol, 4 be cis- 2- hexen-1-ols, and 5 be benzaldehyde, and 6 be the cis- 3- hexenes ester of acetic acid, 7 be acetic acid hexene ester, and 8 be trans- ocimenum, and 9 be furan type linalool oxide, 10 be linalool, and 11 be nonyl alcohol, and 12 be benzyl carbinol, 13 be squalene, and 14 be cis- butyric acid -3- hexene esters, and 15 be pentadecane, 16 be α-farnesene, and 17 areβ-Laurene, 18 be hexadecane.In Fig. 4：1 is trans- 2- hexenoic aldehydes, and 2 be cis- blatter alcohol, and 3 be the cis- 3- hexenes ester of acetic acid, and 4 be 2- ethyl -1- hexanols, and 5 be cis-linalool oxide, and 6 be linalool, and 7 be nonyl alcohol, and 8 be the tetradecane, and 9 be pentadecane, and 10 be hexadecane.In Fig. 5：1 is trans- 2- hexenoic aldehydes, and 2 be cis- blatter alcohol, and 3 be benzaldehyde, and 4 be the cis- 3- hexenes ester of acetic acid, and 5 be trans- ocimenum, and 6 be cis-linalool oxide, and 7 be linalool, and 8 be squalene, and 9 be cis- butyric acid -3- hexene esters, and 10 be pentadecane, and 11 areα- farnesene.
2nd step develops tassel chalcid fly inducing agent
Behavior determination is carried out to tassel chalcid fly using Four-armed olfactomete.The volatile component for inhaling the tea tip caused harm or the tender stem release new stabbed by spawning is pierced by leafhopper, has α-farnesene, benzaldehyde, trans- 2- hexenoic aldehydes, addsβ- farnesene, gaultherolin, cis- jasmone, cis- blatter alcohol, atoleine is solvent, is all respectively 10 by taste source, dosage of this 7 kinds of volatile matters- 4 G/ml, the arm for being connected to Four-armed olfactomete, other three arms connect atoleine（Fig. 6）.10 tassel chalcid flies are surveyed in every kind of taste source, and taste source allure stronger, the tassel chalcid fly holdup time is longer.As a result show：Except cis- blatter alcohol, other 6 kinds of volatile matters have notable attracting action to the rod tassel chalcid fly of leafhopper three（Fig. 7）, also have notable attracting action for small fractured bone tassel chalcid fly（Fig. 8）.In Fig. 7：1 is α-farnesene, and 2 be benzaldehyde, and 3 be cis- jasmone, and 4 areβ- farnesene, 5 be trans- 2- hexenoic aldehydes, and 6 be gaultherolin, and 7 be cis- blatter alcohol；The difference that the smell in each taste source compare with its 3 between the delay rod tassel chalcid fly time of leafhopper three has used a tail variance analysis, then with the conspicuousnesses of Duncan ￠ s test comparing differences (P < 0.05 ).In Fig. 8：1 is α-farnesene, and 2 be benzaldehyde, and 3 be cis- jasmone, and 4 areβ- farnesene, 5 be trans- 2- hexenoic aldehydes, and 6 be gaultherolin, and 7 be cis- blatter alcohol.
Comprehensive analysis, preferably with α-farnesene, cis- jasmone, gaultherolin and benzaldehyde, field concentration is all 10-2 G/ml, then by 1: 0.1
15: 0.1
15: 0.1
15 ratio is made into inducing agent, using n-hexane as solvent.
3rd step develops tassel chalcid fly lure
Referring to Fig. 1, the leaf tea tip of 1 bud 2 is copied with rubber, profile is similar to the real tea tip, when the colour space selects absolute measurement mode（L*a*b* aberration systems）, it is respectively 34 to measure the bud-leaf of the tea tip and the brightness L of tender stem, chromaticity coordinate a, chromaticity coordinate b
52nd, -10.0- 19.0 and 16 23。
The tassel chalcid fly that the rubber tea tip is immersed in the 2nd step is trapped into agent solution 1236 hours, taking-up was dried, and tassel chalcid fly lure is made.Sealing, low tempertaure storage is standby.
4th step traps tassel chalcid fly restriction false eye leafhopper population using tassel chalcid fly lure
The a piece of tea place for not controlling worm throughout the year is selected during autumn tea in 2010 in Jiande City, Zhejiang Province mountain area Ma Mu villages mountain forest, is divided into two parts, a part of area 5
667 m2As area is trapped, another part is also 5 667 m2, as CK areas, Prevention technology is not done, at a distance of 300 meters.
Find that September leafhopper adult population on the 25th enters beginning peak period by insect survey（Total insect population 16% or so is accounted for into borer population）, morning 9:It is 3 m tea rows per sampling point respectively at trapping the 10 points of samplings in area and CK areas checkerboard type when dew is not dry before 00, leafhopper is looked under every 10 buds of sampling point on the 2nd leaf at random into, nymph quantity, 100 leaves are looked into per area.Afternoon 2:00-3:00 is trapping area by 7 m8 m place lure, every 667 m2Put 20,5
667 m2Trap in area place 100.Lure is hung on tea clump upper strata.
After 10 days, i.e. October 5 places lure with method, and area and CK areas leafhopper insect density are trapped with method investigation.
Insect pest situation generaI investigation also found that October 15, leafhopper adult population entered peak period（Total insect population 45%-50% is accounted for into borer population）, morning 9:Before 00 area and CK areas leafhopper insect density, afternoon 2 were trapped with method investigation: 00 ~ 3 :00 is trapping area's placement lure with method.After 15 days, i.e. October 30 traps area and CK areas leafhopper insect density with method investigation.
Also according to insect survey, November 5, leafhopper adult population entered Sheng latter stage（Total insect population 84% or so is accounted for into borer population）, now the density of two kinds of tassel chalcid fly populations is also larger, intends trapping mymaridae parasitized leafhopper, forces down leafhopper and survive the winter insect population.The morning 9 on the 5th:Before 00 area and CK areas leafhopper insect density, afternoon 2 on the 5th were trapped with method investigation: 00 ~ 3 :00 is trapping area's placement lure with method.Morning 9 on May 30th, 2011:00 was former, morning 9 on June 20th, 2011:Before 00 area, CK areas leafhopper insect density were trapped with method investigation.As a result referring to table 3.
Temperature is slightly lower in mountain forest, and humidity is larger, and it is good that tea tree bud-leaf holds tender property.It is all more that the leafhopper adult population on the 25th of September in 2010 enters the adult of leafhopper and nymph quantity in beginning peak period, tea place, super index for control（Blinds insect population >=15）.In CK areas, leafhopper insect population is continuously increased, and to population density maximum on October 15, is gradually reduced again afterwards.Existing a number of tassel chalcid fly in September tea place on the 25th, their parasitic leafhopper can be trapped by placing lure, so trapping area leafhopper insect population has declined；Tassel chalcid fly quantity is also increasing in 5 ~ 15 October tea place.November 5, leafhopper adult entered peak period, and tassel chalcid fly quantity is also increased, and they seek leafhopper ovum, and spawning is survived the winter.Now it is again carried out trapping, has forced down leafhopper insect population in leafhopper hibernating base, trapping areas in 2011 and risen slowly.
1 detection method
Using n-hexane as solvent, 10 are prepared respectively - 2G/ml dosageα- farnesene, benzaldehyde, trans- 2- hexenoic aldehydes,β- farnesene, gaultherolin and cis- jasmone solution, using n-hexane as control, take the benzaldehyde solution and cis- jasmone solution of equivalent to match somebody with somebody resulting mixture.The leaf tea tip of 1 bud 2 that rubber material is copied respectively is dipped in this 8 kinds of solution 24 hours, and taking-up is dried, and lure is made.
Autumn tassel chalcid fly quantity is gradually increased, and selects the Mei Jiawu villages in Hangzhou Xihu Longjing Tea original producton location, September is tested on the 11st within 2010, and 10:30 ~ 12:30 are positioned over each lure in tea place, and each lure invests 1 20 cm
On the green haftplatte of 40 cm bud, the another side of colour table is without lure, and colour table is tethered on small bamboo with thin wire, and small bamboo is inserted in tea clump.Every kind of processing all includes 10 colour tables for having lure, and colour table is according to 7 m
8 m density, bottom and the tea tip is contour is put in tea place.Noon 12 on the 13rd:30 ~ 14:The rod tassel chalcid fly of leafhopper three captured on every haftplatte of the 30 every kind of lures of investigation and the quantity of small fractured bone tassel chalcid fly.The colour table that this experiment is used is sticking, to detectα- farnesene, benzaldehyde, trans- 2- hexenoic aldehydes,β- farnesene, gaultherolin, the allure of cis- jasmone；But, when tassel chalcid fly restriction leafhopper is trapped in field, implementing preventing and treating, colour table used is inviscid.
Proper temperature during experiment, 8:30 ~ 16:30 temperature are maintained at 23 ± 1 DEG C, and relative humidity is maintained at 70 ± 5%.Early, late temperature is relatively low, declines 5 ± 1 DEG C.
As a result with analysis
Referring to Fig. 9,α- farnesene, benzaldehyde, trans- 2- hexenoic aldehydes,β- farnesene, gaultherolin, 6 kinds of tea tree synomones of cis- jasmone have notable attractant effect for two kinds of tassel chalcid flies.When benzaldehyde and cis- jasmone are used in mixed way, allure is significantly increased.This experiment finds that the rod tassel chalcid fly of leafhopper three is sociales, accounts for the 88% of tassel chalcid fly total amount.In Fig. 9：1 isα- farnesene, 2 be benzaldehyde+cis- jasmone, and 3 areβ- farnesene, 4 be gaultherolin, and 5 be cis- jasmone, and 6 be benzaldehyde, and 7 be trans- 2- hexenoic aldehydes, and 8 be n-hexane；Difference between 2 kinds of tassel chalcid fly quantity that 8 kinds of taste sources are lured has used a tail variance analysis, then with the conspicuousnesses of Duncan ￠ s test comparing differences (P < 0.05 )。
Referring to Figure 10, colour table a faces have lure, have trapped more tassel chalcid fly；The not attached lure in colour table b faces, the tassel chalcid fly trapped is less, and such quantity variance illustrates that more tassel chalcid fly is intended to lure, and lure has seduction.In Figure 10：The tassel chalcid fly average of a -80 piece colour table a faces trapping
Standard deviation；The tassel chalcid fly average of b -80 piece colour table b faces trapping
Standard deviation.
Parasitic rate of the detection inducing agent lifting tassel chalcid fly of embodiment 3 for false eye leafhopper ovum
1 detection method
In organic tea garden of on the October 3rd, 2010 in the Mt. Jingting scenic spot on Anhui Province Mt. Jingting tea plantation, the rod tassel chalcid fly of leafhopper three and small fractured bone tassel chalcid fly have been adopted.Then this piece organic tea garden is divided into two parts, a part of area about 5 of uptake
667 m2, as area is trapped, another part area in lower air port is about 5
667 m2As CK areas, lure is not placed, at a distance of 300 m.
In morning October 3, there is the leaf tea tip of 1 bud 5 of spawning puneture on random clip tender stem surface from two parts tea place respectively, returns to interior, sets aside barbing trace in anatomical lens and takes out leafhopper ovum, is placed on the moistening filter paper in big culture dish, culture dish is placed in illumination box.25 ± 3 DEG C of illumination box temperature, the % of relative humidity 70 ± 5, illumination 32000
36000 lux, photoperiod 14: 10 (L : D).It is placed in good time under stereoscope, observation leafhopper ovum is by sneak case, observation by the change of tassel chalcid fly body colour and the bodily form in parasitic leafhopper ovum.Afternoon October 3 is just trapping area's suspension tassel chalcid fly lure, lure suspension tea tip upper strata, according to 7 m8 m density, every 667 m2Suspension 20,5
667 m2Trap in area hang 100.Afternoon on October 13rd, 2010 is trapping area's suspension tassel chalcid fly lure with method again.
2 results and analysis
In afternoon on October 23rd, 2010, there is the leaf tea tip of 1 bud 5 of spawning puneture trapping area, CK areas collection tender stem surface with method, return to and indoor observe parasitic rate with method.As a result such as table 4.It is taken as that：Inducing agent can effectively trap tassel chalcid fly within a certain period of time, and improve its parasitic rate for leafhopper ovum.
Claims (4)
1. a kind of method of trapping mymaridae parasitized empoasca vitis, it is characterised in that realized by following steps：
（1）From α-farnesene, cis- jasmone, 4 kinds of synomones of gaultherolin and benzaldehyde as taste source, every kind of taste source concentration is all 10-2G/ml, using n-hexane as solvent, by 1: 0.1~15: 0.1~15:0.1 ~ 15 ratio is made into tassel chalcid fly and traps agent solution；
（2）The 1 bud 2 leaf tea tip is copied with rubber, the is uploaded to（1）The inducing agent of step, is made tassel chalcid fly lure；
（3）Latter stage is contained in leafhopper population beginning peak period, leafhopper population full incidence period and leafhopper population, lure, trapping mymaridae parasitized empoasca vitis ovum are placed in tea place.
2. a kind of method of trapping mymaridae parasitized empoasca vitis according to claim 1, it is characterised in that step（2）Be by the tea tip immersion concentration be 10-2G/ml tassel chalcid fly is trapped in agent solution 12 ~ 36 hours, and taking-up is dried, and tassel chalcid fly lure is made, and sealing, low tempertaure storage is standby.
3. a kind of method of trapping mymaridae parasitized empoasca vitis according to claim 1, it is characterised in that step（3）It is that lure is hung on tea clump upper strata.
4. a kind of application of the method for trapping mymaridae parasitized empoasca vitis according to claim in the rod tassel chalcid fly of leafhopper in trapping tea place three and the parasitic false eye leafhopper ovum of small fractured bone tassel chalcid fly.
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| CN111567521A (en) * | 2020-05-21 | 2020-08-25 | 中国农业科学院植物保护研究所 | Plant-derived volatile matter composition for trapping codling moth, trapping core and application |
| CN111567521B (en) * | 2020-05-21 | 2021-07-20 | 中国农业科学院植物保护研究所 | Plant-derived volatile matter composition for trapping codling moth, trapping core and application |
| CN113875753A (en) * | 2021-10-18 | 2022-01-04 | 杭州茶菊科技有限公司 | Attractant for three-rod-tassel apis cerana and tiny schizochytrix delavayi and application thereof |
| CN113875753B (en) * | 2021-10-18 | 2022-07-22 | 杭州茶菊科技有限公司 | Attractant for three-rod-tassel apis cerana and tiny schizochytrix delavayi and application thereof |
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