CN1593122A - Tea garden insect trapping method - Google Patents
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- CN1593122A CN1593122A CN 200410018059 CN200410018059A CN1593122A CN 1593122 A CN1593122 A CN 1593122A CN 200410018059 CN200410018059 CN 200410018059 CN 200410018059 A CN200410018059 A CN 200410018059A CN 1593122 A CN1593122 A CN 1593122A
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Abstract
The invention discloses a method for trapping tea plantation insects comprising the steps of, (1) separating and determining 10 pest damage volatile matters by adopting adsorptive separation method, (2) determining four volatile constituents with inducement activity by biological assay method, (3) preparing four active constituents into flavor source substances, matching the four flavor source substances, so as to formulate the insect attractant, (4) determining three colors with inducement effect to insects by tea plantation small zone inducement experiments, (5) combining the bud greening color with the attractant to prepare insect trapper, and (6) placing the trapper into the field to trap the insects.
Description
Technical field
The present invention relates to a kind of tea place natural enemy insect method for enticing collection.
Background technology
China is the motherland of tealeaves, and the tree plant cultivation of China is with a long history.Found out that in recent years China has kind surplus the tea tree insect 800, wherein 5% (30～45 kinds) are the normal important pests of sending out property.Tea tree insect conception of comprehensive has proposed decades, also constantly obtains abundant and development in controlling the worm practice, yet, main dependence chemical control in the tea tree control of insect of reality.Usually, the annual chemoprevention of some tea plantations and peasant household more than 10 time during the summer autumn tea, will be executed medicine 1 time in per 7～10 days, be easy to cause residue of pesticide, also can cause insect and develop immunity to drugs, and tea place food web, chain structure are tending towards merely, naturally the reduction of control potential.The residual bottleneck that was once becoming restriction China tea export of tealeaves farming.European Union, Japan etc. are increasing the residual test item to China outlet tealeaves middle peasant again, and tens times～hundreds of times ground reduces the residual maximum detection limit of tealeaves farming.Therefore, must expand and control the worm new way.
Since the nineties, the chemical communication mechanism between host plant-insect-natural enemy three trophic levels becomes the research focus, and semiochemical carrier is a semiochemicals.The insect chemistry information substance is pollution-free, using dosage is small (mg～μ g level), the activity strong.The synomone that kairomone that insect discharges and insect pest plant discharge all can attract natural enemy effectively, is exploring in the world always and is using this class material innoxious governance insect
[1,2]Yet, breakthrough research seldom, the rarely seen report of larger area successful Application.Insect generally all has phototaxis
[3], Wang Yong etc. (1991)
[4]Report khaki ticker tape has seduction to the tea geometrid larva.Xiao Sunv (1997)
[5]Studied the attracting action of white, blue, yellow, yellowish green, blackish green 5 kinds of colour tables to tea lesser leafhopper Jacobiasca formosana Paoli and Aleurocanthus spiniferus Aleurocanthus spiniferus (Quaintance), find the effective of the sticking paper trapping of yellow green leafhopper, yellow paper is effective to Aleurocanthus spiniferus.Yet, do not see the report of luring natural enemy in the tea place with color as yet.
Tea tree is the perennial evergreen shrubbery, closing in the tea clump, and the habitat is stable, is concealing multiple natural enemy insect.The natural enemy insect of having put down in writing has hundreds of.Common monoid has cocoon honeybee, ladybug, syrphus fly, robber fly, ichneumon wasp, Chrysopa, or the like.If insect pheromone and color combined make trapper, discharge in certain insect emergence period, then might lure a lot of natural enemies of the collection Pest Control of coming, chemical pesticide is used in deduction and exemption, and promotes organic tea and nuisanceless tealeaves production.
List of references
[1]Dicke，M.1994.Local?and?systemic?production?of?volatilesherbivore-induced?terpenoids：their?role?in?plant-
carnivore?mutualism.J.Plant?Physiol.143：465-472
[2]Vet，L.E.M.，and?Dicke，M.1992.Ecology?of?infochemical?useby?natural?enemies?in?a?tritrophic?context.Ann.
Rev.Entomol.37：141-172
[3] Chen Ningsheng. visual communication. see: Chinese agriculture encyclopedia insect roller editorial board compiles, Chinese agriculture encyclopedia insect roller. the agriculture .1990.362 of publishing house～363
[4] Wang Yong, Zhang Hangu, Zou Yunding. ticker tape lures the collection Study on Efficiency to the tea geometrid larva. plant protection journal, 1991,18 (2): 177～180
[5] Xiao Sunv. the effect research of coloured sticking paper trapping pests in the tea place. Tea Industry research in Taiwan is reported, and 1997,16:51～60
[6] compile and edit Chinese Academy of Sciences's compiling publication committee noun chamber. chromatogram. and Beijing: the .1957 of Science Press
Summary of the invention
The purpose of this invention is to provide a kind of tea place natural enemy insect method for enticing collection.
The step of method is: 1) adopt the absorption method isolation identification to go out 10 kinds of insect pest tea shoot volatiles, it is benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid, 2) adopt bioassay method to determine benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin is the volatile component with attractive activity, 3) with benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin is made into the odor source that concentration is 0.01-20 μ g/ml respectively, again 4 kinds of odor sources are pressed 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, the attractant of natural enemy insect is lured in preparation, 4) it is green to adopt sub-district, tea place trapping experiment to determine natural enemy insect is had the parrot of seduction from 10 kinds of colors, green and the yellow 3 kinds of colors of jasmine of bud, 5) with bud green and benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, the combination of gaultherolin attractant, make the trapper of natural enemy insect, 6) trapper is put into the field lure the collection natural enemy insect.
Advantage of the present invention:
1) pollution-free, be adapted to current organic tea and nuisanceless tealeaves production;
2) efficient height, stable performance.Just natural enemy can be attracted Pest Control in the of short duration time, reduce or remit and use chemical pesticide;
3) be convenient to transportation, easy to use;
4) with low cost.
Description of drawings
Accompanying drawing is a trapper structural representation of the present invention.
Embodiment
1, adopt the absorption method isolation identification to go out 10 kinds of insect pest tea shoot volatiles, i.e. benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid
Make two and have the glass column that bore is 6～10 millimeters tiny openings, hollow, 4.5～5.5 liters of volumes, import connects compressed air bottle, and outlet connects Tenax TA adsorption column, vavuum pump successively.Before the adsorbing volatilizing thing, at 270～280 ℃, logical nitrogen activates 4 hours 4～8 hours with Tenax TA post.Add 190～210 gram tea aphids harm tea tips in glass container, be blown into air, bleed behind adsorption column simultaneously, control flow with flowmeter before import, behind the Tenax TA post, the inlet, outlet flow all is 300 ml/min.Take off adsorption column after 11～13 hours,, slowly purge with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component to 15～30 microlitres with heavily steaming ether drip washing.The tea aphid 10 kinds of chemical constituents in the tea shoot volatile that cause harm have been identified: benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid.Wherein anti--2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin are the big component of content.
2, determine the synomone that attractive activity is strong with bioassay method
1) " Y " shape olfactometer
" Y " shape manages two arms and base portion length is 10cm, and two arm angles are 90 °, connect flavor source bottle (or control bottle), distilled water bottle, activated carbon bottle successively.With the n-hexane is solvent, and wiring solution-formings are as odor source respectively with 10 kinds of aphid evils such as anti--2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin tea shoot volatiles, and concentration is 0.01 μ g/ml, 1 μ g/ml.With n-hexane is contrast.The odor source of containing 1ml with the 2ml peace a word used in place name bottle of opening places the n-hexane of flavor source bottle, Sheng 1ml to place control bottle.Bleed with vavuum pump from Y pipe base portion, natural air through active carbon filtration, distilled water bottle humidification, flavor source bottle (or control bottle), is managed two arms at Y and is crossed successively, enters Y pipe base portion.Stop to bleed, introduce 9 kinds of natural enemy insects by head respectively from Y pipe base portion opening: aphid cocoon honeybee Aphidius sp., the obvious mutation L.axyridis of harmonia axyridia (Pallas) var.spectabilisFaldermaenn, harmonia axyridia manifests mutation L.axyridis (Pallas) ab.conspicua Faldermaenn, harmonia axyridia 19 spot mutation, harmonia axyridia two spot modification L.axyridis (Pallas) ab.bimaculataHemmelmann, coccinella septempunctata Coccinella septempunctata L., Chinese green lacewing Chrysopa sinicaTjedea, chrysopa septempunctata Chrysopa septempunctata Wesmeal and Men Shi syrphus fly Sphaerophoriamenthastri L..After the introducing, bleed, insect advances contrary wind again, manages two arm intersections at Y and stops slightly, selects an arm wherein to enter.
Counting is selected the natural enemy insect number of flavor source and n-hexane.Use χ
2Method detects the difference between the insect number of selecting the flavor source and the insect number of the selecting n-hexane.Find that 9 kinds show tangible taxis for the examination insect to benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, trend is anti--and the insect number and the trend of 2-hexenoic aldehyde, benzaldehyde, gaultherolin contrast that difference reaches significance level (P＜0.05) between the insect number of (n-hexane).
2) insect tentaculum electric potential instrument
For the examination volatile matter is 10 kinds of insect pest tea shoot volatiles such as benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin.Concentration is 0.01 μ g/ml, 1 μ g/ml, and n-hexane is a solvent.And be contrast with the n-hexane.For the examination natural enemy insect is the above-mentioned 9 kinds of natural enemy insects that pick up from the tea place.The insect tentaculum electric potential is reflected at Peking University's biomembrane and membrane-biotechnology National Key Laboratory finishes.The result shows, benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin can cause bigger tentaculum electric potential reaction.
3, benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin are made into the odor source of concentration for (0.01-20) μ g/ml respectively, again with 4 kinds of odor sources by 1: 0.5-1.5: 0.2-1.2: the 0.3-1.5 assembly, the attractant of natural enemy insect is lured in preparation
With n-hexane is solvent, benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin are made into the odor source that concentration is 0.01-20 μ g/ml respectively, again 4 kinds of odor sources are pressed 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, the biologicall test of being done through " Y " shape olfactometer shows that attractant effect is stronger.Here it is tea place natural enemy insect attractant.
With the rubber tip that the soaked in absolute ethyl alcohol natural rubber is made, dry.Soak such rubber tip with attractant again, just made and lured core.
4, adopt sub-district, tea place trapping experiment to determine from 10 kinds of colors natural enemy insect is had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine
1) color that allure is strong is determined in field plot trial
Purchase that bud is green, parrot is green, jasmine is yellow, rape is brightly yellowish, olive is yellowish green, calcedony is green, peacock green, bluish-green, blackish green and snow-white 10 kinds of ticker tapes, every kind of color is all made the two-sided colour table of 10 block length 40cm, wide 20cm, colourless insect glue is smeared on the two sides of colour table equably, places the tea place to lure natural enemy.The result shows that the attractant effect that bud is green, jasmine is yellow, parrot is green is stronger.
2) parameter of 3 kinds of strong allure colors of mensuration
The color colour difference meter (color difference meter) that uses Hong Kong Minolta company to produce, model C R-331C, the colour space is selected absolute measurement mode (L for use
*a
*b
*The aberration system).Record:
The green L69.00 of parrot (67～70) a-43.57 (40～-47) b+44.27 (+40～447)
The green L75.42 of bud (73～75) a-40.57 (40～-47) b+47.43 (+44～+ 50)
Yellow L86.96 (84～90) a-5.84 (2～-9) b+64.77 (+6 1～+ 68) of jasmine
Wherein the 1st numerical value L represents brightness; A, b are chromaticity coordinates, and the 2nd numerical value "+" is represented red composition, and "-" represents green composition; The 3rd numerical value "+" expression Huang Cheng part, "-" represents blue composition.
5,, make the trapper of natural enemy insect with bud green and anti--2-hexenoic aldehyde, benzaldehyde, indoles, the combination of gaultherolin attractant
The trapper that lures collection tea place natural enemy insect is that the green colour table of bud adds the core that lures that is loaded with attractant.Attractant is made into the odor source that concentration is 0.01-20 μ g/ml respectively by benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, and these 4 kinds of odor source solution are again by 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, solvent are n-hexane.
6, trapper is put into the field and lured the collection natural enemy insect
The natural enemy insect trapper is positioned in the tea place, and the trapper lower limb is concordant with the tea tip, and the distance between the trapper is (4～8) rice.
7, embodiment
Embodiment 1 adopts the absorption method isolation identification to go out 10 kinds of insect pest tea shoot volatiles, i.e. benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid
Make two and have the glass column that bore is 7 millimeters tiny openings, hollow, 5 liters of volumes, import connects compressed air bottle, and outlet connects Tenax TA adsorption column, vavuum pump successively.Before the adsorbing volatilizing thing, the TenaxTA post is activated 4 hours at 275 ℃ of logical nitrogen.Add the 200 gram tea aphids harm tea tips in glass container, be blown into air, bleed behind adsorption column simultaneously, control flow with flowmeter before import, behind the Tenax TA post, the inlet, outlet flow all is 300 ml/min.
Take off adsorption column after 12 hours,, slowly purge with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component to 20 microlitres with heavily steaming ether drip washing.
The tea aphid 10 kinds of chemical constituents in the tea shoot volatile that cause harm have been identified: benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid.Wherein anti--2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin are the big component of content.
Embodiment 2 adopts bioassay methods to determine that benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin are the volatile component with attractive activity
According to embodiment 2, with concentration be the flavor source of 1 μ g/ml measure 9 kinds of natural enemy insects in olfactometer to 10 kinds of insect pest volatile matters behavior reaction.Counting is selected the natural enemy insect number of flavor source and n-hexane.Use χ
2Method detects the difference between the insect number of selecting the flavor source and the insect number of the selecting n-hexane.Find that 9 kinds show tangible taxis for the examination insect to benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, trend is anti--and the insect number and the trend of 2-hexenoic aldehyde, benzaldehyde, gaultherolin contrast that difference reaches significance level (P＜0.05) between the insect number of (n-hexane).
According to embodiment 2, utilize the tentaculum electric potential instrument, measuring 9 kinds of natural enemy insects is the electrophysiologic response of 10 kinds of insect pest tea shoot volatiles of 1 μ g/ml to concentration.And be contrast with the n-hexane.The insect tentaculum electric potential is reflected at Peking University's biomembrane and membrane-biotechnology National Key Laboratory finishes.The result shows, benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin can cause bigger tentaculum electric potential reaction.
Embodiment 3 is made into the odor source that concentration is 0.01-20 μ g/ml respectively with benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin, again with 4 kinds of odor sources by 1: 0.5-1.5: 0.2-1.2: the 0.3-1.5 assembly, the attractant of natural enemy insect is lured in preparation
With the n-hexane is solvent, and benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin are made into the odor source that concentration is 1 μ g/ml respectively, and these 4 kinds of odor source solution are again by 1: 1: 1: 1 assembly.The rubber tip made from the soaked in absolute ethyl alcohol natural rubber 24 hours is dried.Soak rubber tip 24 hours with attractant again, dry, just made the natural enemy attractant.
Embodiment 4 adopts sub-district, tea place trapping experiments to determine from 10 kinds of colors natural enemy insect is had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine
It is described to press embodiment 4, makes the viscose glue plate of 10 colors.
Natural enemy is lured in the tealeaves company tea place of being bestowed by heaven in the Beilun,Ningbo City, Zhejiang Province town on October 22nd, 2002, bud is green, parrot is green, jasmine is yellow, rape is brightly yellowish, olive is yellowish green, calcedony is green, the average every block of plate of peacock green, bluish-green, blackish green, snow-white 10 kinds of colour tables lures to such an extent that all kinds of natural enemy numbers are respectively: 18 ± 8,15 ± 9,14 ± 9,14 ± 8,13 ± 8,12 ± 7,10 ± 7,8 ± 4,6 ± 4 and 5 ± 3.
On May 30th, 2003, the fiber crops Gu Shanchachang of Anhui Province test, bud is green, parrot is green, jasmine is yellow, rape is brightly yellowish, olive is yellowish green, calcedony is green, the average every block of plate of peacock green, bluish-green, blackish green, snow-white 10 kinds of colour tables lures to such an extent that all kinds of natural enemy numbers are respectively: 16 ± 8,15 ± 6,15 ± 7,15 ± 8,12 ± 5,11 ± 4,10 ± 4,7 ± 3,6 ± 3 and 5 ± 3.
Above-mentioned test shows that bud is green, parrot is green, the yellow three kinds of color allures of jasmine are stronger.
Embodiment 5 with bud green with benzaldehyde, anti--the 2-hexenoic aldehyde, benzaldehyde, indoles, the combination of gaultherolin attractant, make the trapper of natural enemy insect
According to embodiment 5, with benzaldehyde, anti--the 2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin attractant (concentration 1ug/ml, proportioning 1: 1: 1:, make the trapper of natural enemy insect 1) with jasmine Huang, green, the green combination of parrot of bud.
Selected the woodland tea place of the fiber crops Gu Shanchachang of Anhui Province on August 26th, 2003,50 mu of areas.Choose heart zone to be the test region, with green 5 of yellow 5 of jasmine, bud green 5 and parrot, hang up the core that lures of luring natural enemy respectively, spacing is 5m * 5m.Investigation on the 28th, 5 blocks of plates lure collection natural enemy number: bud is green 53, parrot is green 51, jasmine Huang 50.The average every block of plate of investigation on the 30th lures collection natural enemy number: bud is green 24, parrot is green 21, jasmine Huang 20.
Selected the nuisanceless test tea place of the fiber crops Gu Shanchachang of Anhui Province on September 11st, 2003,30 mu of areas.Choose heart zone to be the test region, with green 5 of yellow 5 of jasmine, bud green 5 and parrot, hang up the core that lures of luring natural enemy respectively, spacing is 5m * 5m.Looked into effect on the 13rd, 5 blocks of plates lure collection natural enemy number: bud is green 113, parrot is green 60, jasmine Huang 72.
The above results shows that the green trapper made from the attractant combination of bud lures the collection effect best.
Embodiment 6 puts into the field with trapper and lures the collection natural enemy insect
Selected conventional production tea place, Anhui Province fiber crops aunt camellia field on October 23rd, 2003, the green plate of bud hang up be loaded with the natural enemy attractant (concentration is 1ug/ml, and proportioning is 1: 1: 1: 1) lure core as luring device, totally 20; Selecting the green plate of bud to hang up heavily to steam that n-hexane soaks lures core in contrast for use, also is 20.40 trappers are distributed in the tea place randomly, and trapper lower limb and tea shoot are joined.Looked into attractant effect on October 25.
1) the natural enemy trapper is compared with the contrast trapper
The natural enemy trapper lure total species several 33; Total individual number 348, average every trapper lure natural enemy number 17.4 ± 7.1 contrast trappers lure total species several 28; Total individual number 222, average every trapper lure natural enemy number 11.6 ± 6.3.
With " comparative analysis of burst data " method, lure to such an extent that the natural enemy number lures to such an extent that the natural enemy number is carried out the t test with the contrast trapper for the natural enemy trapper, record difference and reach utmost point significance level (P＜0.01).
2) natural enemy trapper front (extension lures core) compared with reverse side
Positive natural enemy kind is several 29, average every trapper lure several 5.1 ± 1.8 kinds of natural enemy kind; The natural enemy kind of reverse side is several 21, average every trapper lure several 3.0 ± 1.8 kinds of natural enemy kind.With " comparative analysis of paired data " method, for natural enemy trapper and contrast trapper lure the kind number carry out t test, record difference and reach utmost point significance level (P＜0.01).
Positive natural enemy number 246, on average each trapper lures to such an extent that the natural enemy number is 12.3 ± 5.3; The natural enemy number 102 of reverse side, average every trapper lure the natural enemy number for being 5.1 ± 4.2.With " comparative analysis of paired data " method, carry out the t test for the two, record difference and reach utmost point significance level (P＜0.01).
Claims (11)
1, a kind of tea place natural enemy insect method for enticing collection, it is characterized in that, the step of method is: 1) adopt the absorption method isolation identification to go out 10 kinds of insect pest tea shoot volatiles, it is benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid, 2) adopt bioassay method to determine benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin is the volatile component with attractive activity, 3) with benzaldehyde, with anti--2-hexenoic aldehyde, indoles, gaultherolin is made into the odor source that concentration is 0.01-20 μ g/ml respectively, again 4 kinds of odor sources are pressed 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, the attractant of natural enemy insect is lured in preparation, 4) it is green to adopt sub-district, tea place trapping experiment to determine natural enemy insect is had the parrot of seduction from 10 kinds of colors, green and the yellow 3 kinds of colors of jasmine of bud, 5) green and anti--2-hexenoic aldehyde with bud, benzaldehyde, indoles, the combination of gaultherolin attractant, make the trapper of natural enemy insect, 6) trapper is put into the field lure the collection natural enemy insect.
2, a kind of tea place according to claim 1 natural enemy insect is lured method, it is characterized in that said employing absorption method isolation identification goes out 10 kinds of insect pest tea shoot volatiles, it is benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--2-hexenoic acid: it is 6～10 millimeters tiny openings that making two has bore, the glass column of hollow, 4.5～5.5 liters of volumes, import connects compressed air bottle, outlet connects the TenaxTA adsorption column successively, vavuum pump, before the adsorbing volatilizing thing, Tenax TA post is activated 4～8 hours at 270～280 ℃ of logical nitrogen; In glass container, add 190～210 gram tea aphids tea tip of causing harm, be blown into air, behind adsorption column, bleed simultaneously, before import, control flow with flowmeter behind the TenaxTA post, take off adsorption column after 11～13 hours, with heavily steaming ether drip washing, slowly purge to 15～30 microlitres with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component, identified benzaldehyde in the aphid evil tea shoot volatile, instead-the 2-hexenoic aldehyde, indoles, gaultherolin, ocimene, suitable-3-acetate hexene ester, suitable-blatter alcohol, linalool, geraniol and anti--10 kinds of chemical constituents of 2-hexenoic acid, wherein anti--2-hexenoic aldehyde, benzaldehyde, indoles and gaultherolin are the big components of content.
3, a kind of tea place according to claim 1 natural enemy insect is lured method, it is characterized in that said employing bioassay method determines that benzaldehyde, anti--the 2-hexenoic aldehyde, indoles, gaultherolin are the volatile component with attractive activity: 1) adopt " Y " shape olfactometer to determine attractive activity component, 2 in the synomone) adopt insect tentaculum electric potential instrument to determine attractive activity component in the synomone.
4, a kind of tea place according to claim 3 natural enemy insect is lured method, it is characterized in that said employing " Y " shape olfactometer determines attractive activity component in the synomone: 1) two arms and the base portion length of " Y " shape pipe are 9～11cm, two arm angles are 90～110 °, connect flavor source bottle or control bottle, distilled water bottle, activated carbon bottle successively, bleed with vavuum pump from Y pipe base portion, natural air is successively through active carbon filtration, distilled water bottle humidification, flavor source bottle or control bottle, cross in arm crossing place of Y pipe, enter Y pipe base portion.Stop to bleed, introduce aphid cocoon honeybee, harmonia axyridia 4 mutation, coccinella septempunctata, Chinese green lacewing, chrysopa septempunctata and 9 kinds of natural enemy insects of syrphus fly respectively from Y pipe base portion opening, bleed, insect is advanced against the wind, manage two arm intersections at Y and stop slightly, select an arm wherein to enter; 2) with the n-hexane be solvent, wiring solution-forming is as odor source respectively with 10 kinds of aphid evil tea shoot volatiles, and concentration is 0.01～20 μ g/ml, is contrast with the n-hexane.The odor source of containing 1ml with the 2ml peace a word used in place name bottle of opening places the n-hexane of flavor source bottle, Sheng 1ml to place control bottle, introduces natural enemy insect by head, bleeds the insect number of statistics selection flavor source and n-hexane; 3) use χ
2Method detects insect number that tends to the flavor source and the difference between the insect number that tends to n-hexane, find: 9 kinds for the examination insect to instead-2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin show tangible taxis, trend is anti--insect number that the insect number and the trend of 2-hexenoic aldehyde, benzaldehyde, gaultherolin contrasts between difference P＜0.05.
5, lure method according to claim 3 or 4 described a kind of tea place natural enemy insects, it is characterized in that said employing insect tentaculum electric potential instrument determines attractive activity component in the synomone: be 10 aphids such as anti--2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin evil tea shoot volatiles for the examination volatile matter.Concentration is 0.01～20 μ g/ml, and n-hexane is a solvent, and is contrast with the n-hexane, and aphid cocoon honeybee, harmonia axyridia 4 mutation, coccinella septempunctata, Chinese green lacewing, chrysopa septempunctata are for supplying the examination insect.
6, a kind of tea place according to claim 1 natural enemy insect is lured method, it is characterized in that said with benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin is made into the odor source that concentration is 0.01-20 μ g/ml respectively, again 4 kinds of odor sources are pressed 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, the attractant of natural enemy insect is lured in preparation: be solvent with n-hexane 1), with anti--2-hexenoic aldehyde, benzaldehyde, indoles, gaultherolin is made into the odor source that concentration is 0.01-20 μ g/ml respectively, again 4 kinds of odor sources is pressed 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly; 2) rubber tip made from the soaked in absolute ethyl alcohol natural rubber was dried more than 24 hours, soaked such rubber tip with attractant again, had just made and had lured core.
7, a kind of tea place according to claim 1 natural enemy insect is lured method, and it is characterized in that sub-district, said employing tea place trapping experiment determines from 10 kinds of colors natural enemy insect is had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine: 1) color, 2 of allure is determined in field plot trial) measure the parameter of 3 kinds of allure colors.
8, a kind of tea place according to claim 7 natural enemy insect is lured method, it is characterized in that said field plot trial determines the color of allure; Sub-district, the field certain parrot of trapping test is green, bud is green and the yellow color that is defined as allure of jasmine.
9, a kind of tea place according to claim 7 natural enemy insect is lured method, it is characterized in that the parameter of 3 kinds of strong allure colors of said mensuration:
Use the color colour difference meter, select for use absolute color space measurement mode to record:
The green L67 of parrot～70 a-40～-47 b+40～+ 47
The green L73 of bud～75 a-40～-47 b+44～+ 50
The yellow L84 of jasmine～90 a-2～-9 b+61～+ 68
Wherein the 1st numerical value L represents brightness; The 2nd numerical value a, the 3rd numerical value b are chromaticity coordinates, and the 2nd numerical value "+" is represented red composition, and "-" represents green composition; The 3rd numerical value "+" expression Huang Cheng part, "-" represents blue composition.
10, a kind of tea place according to claim 1 natural enemy insect is lured method, it is characterized in that said with bud green and benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, the combination of gaultherolin attractant, make the trapper of natural enemy insect: the trapper of luring the tea place natural enemy insect is that the green colour table of bud adds the core that lures that is loaded with attractant, attractant is by benzaldehyde, instead-the 2-hexenoic aldehyde, indoles, gaultherolin is made into the odor source that concentration is 0.01-20 μ g/ml respectively, these 4 kinds of odor source solution are again by 1: 0.5-1.5: 0.2-1.2: 0.3-1.5 assembly, solvent are n-hexane.
11, a kind of tea place according to claim 1 natural enemy insect is lured method, it is characterized in that saidly trapper is put into the field luring the collection natural enemy insect: the described natural enemy insect trapper of claim 10 is positioned over the tea place, the trapper bottom is concordant with the tea tip, and the distance between the trapper is 4～8 meters.
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