CN108770848A - A kind of botanical attractant of Encarsia formosa and its application - Google Patents
A kind of botanical attractant of Encarsia formosa and its application Download PDFInfo
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- CN108770848A CN108770848A CN201810541730.0A CN201810541730A CN108770848A CN 108770848 A CN108770848 A CN 108770848A CN 201810541730 A CN201810541730 A CN 201810541730A CN 108770848 A CN108770848 A CN 108770848A
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- attractant
- encarsia formosa
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of botanical attractant of Encarsia formosa and its applications, which is characterized in that the botanical attractant active constituent of the Encarsia formosa is by (E)-β-ocimenums, beta-myrcene, gaultherolin, β-carypohyllene composition.The invention also discloses application of the botanical attractant of Encarsia formosa in terms of field of pest control, the botanical attractant of the Encarsia formosa of the present invention can be added drop-wise to the release that attractant slow release is made in the lure with slow releasing function, attractant release is dispersed evenly to field, the field diffusivity of Encarsia formosa can be effectively improved, the parasitic rate to Bemisia tabaci Nymph is improved.Attractant preparation method of the present invention is simple, compared with planting plants trap strips, reduces complicated, the suitable large-area applications of cultivated land occupancy and farming operations；The present invention can reduce dosage, effectively reduce the pesticide residue in ecological environmental pollution and crops, have significant ecological benefits and social benefit.
Description
Technical field
The present invention relates to the botanical attractants and its application of plant protection art more particularly to a kind of Encarsia formosa.
Background technology
Encarsia formosa Encarsia formosa Gahan belong to Hymenoptera aphid chalcid fly Koln Aphelinus, are Important Agricultural evils
Worm Bemisia tabaci Bemisia tabaci (Gennadius) and Trialeurodes vaporariorum Westwood Trialeurodes vaporariorum
(Westwood) parasite can effectively control its harm.Since Bemisia tabaci and Trialeurodes vaporariorum Westwood host plant are extensive,
Harm is serious, and drug resistance generates rapidly, therefore just becomes the hot spot of countries in the world research using biological prevention.It is existing to grind
Study carefully and shows that Encarsia formosa can successfully control causing harm for Trialeurodes vaporariorum Westwood, and in recent years, as the invasion of Type B Bemisia tabaci is broken out,
The research of Type B Bemisia tabaci is controlled using parasites such as Encarsia formosas also becomes new hot spot.
Plant volatile plays an important role in the host location of parasitic wasp and selection course.Plant-feed insect can
Its host location behavior is instructed with the volatile matter discharged using host plant, to correctly finding its host plant.Such as
Aphid parasite Aphidius ervi can carry out host using the volatile component 6-methyl-5-hepten-2-one in broad bean
The positioning (Du et al., 1998) of aphid；It is fixed that Suo Nuo crimps ichneumon wasp Campoletis sonorensis have cotton leaf smell
It is (the Elzen et al., 1984) that host is found by the reaction to cotton smell to reaction；Parasitic wasp Cotesia
Marginiventris relies on terpene substances (E- β-farnesene, the E- ɑ-in herbivore-induced plant volatiles
Bergamotene the plant (Schnee et al., 2006) caused harm) is found.Insect pest induces the volatility time that plant generates
Biomass (herbivore-induced plant volatiles, HIPVs), is that plant cooperates with for a long time with plant-feed insect
As a result.And parasitic wasp is during Long Term Contact insect pest host plant, by its learning behavior by herbivore-induced volatile with
Host connects, and carries out New host records and positioning eventually by the specialized plant volatile matter induced using insect pest
(Steinberg et al.,1993；Turlings,1994).Plant hormone (such as external source jasmine is utilized in natural ecosystems
Acid) induction or manual simulation discharge insect pest induce plant volatile, be remarkably improved predation of the natural enemy insect to plant eater
Rate, effectively control insect pest (Thaler, 1999；Kessler and Baldwin 2001).Herbivore-induced plant volatiles master
Component is wanted to have terpenoid, 4 major class of greenery class smell, aromatic compounds and other chemical substances.Numerous studies prove,
Plant volatiles played in parasitic wasp host foraging key effect, especially host kairomone and insect pest induction
Specific host plant volatile component is the main clue that parasitic wasp finds host.It is previous studies have shown that host kairomone or
Its related substances can effectively attract Encarsia formosa orientation flight；In short distance, the volatile matter and vision of host plant are derived from
The combination of signal can cause the transformation of Encarsia formosa flight and landing site.Birkett etc. (2003) comparative analysis health
And Trialeurodes vaporariorum Westwood cause harm after Kidney bean plant volatile, as a result, it has been found that the kidney bean volatiless component to be caused harm by Trialeurodes vaporariorum Westwood
There is more than 20 kinds.Wherein, insect pest plant release (Z) -3-hexen-1-ol, 4,8-dimethyl-1,3,7-nonatriene and
3-octanone contents are higher than healthy plant, and one-component in these compounds or mixture can be to parasitisms
Bee generates stronger sucking action.
Invention content
The purpose of the present invention is during biological control, insufficient for Encarsia formosa diffusivity, parasitic rate is not high
In the case of, design synthesizes a kind of botanical attractant, and provides application of the attractant in terms of field of pest control.
The present invention completes by the following technical programs：
A kind of botanical attractant of Encarsia formosa, including following component：(E)-β-ocimenums, beta-myrcene, salicylic acid
Methyl esters, β-carypohyllene.The weight ratio of wherein described (E)-β-ocimenums, beta-myrcene, gaultherolin, β-carypohyllene is (1-
4):(1-3):(1-2):1.The botanical attractant of the Encarsia formosa further includes having the antioxygen for accounting for total constituent mass 1-5%
Agent and the uvioresistant stabilizer for accounting for total constituent mass 1-5%.The antioxidant of the present invention is used for the antioxygen of botanical attractant
Change, antioxidant can be di-t-butyl hydroxyl cresols (BHT)/butylhydroxy anisole (BHA).
The uvioresistant stabilizer of the present invention is used to improve the stability of botanical attractant, and uvioresistant stabilizer can be
2- (2h- benzotriazole -2- bases) two tert-amyl phenols of -4,6-/2- (the chloro- 2H- benzotriazole -2- bases of 5-) -6- (1,1- dimethyl
Ethyl) -4- methylphenols.
The botanical attractant of the Encarsia formosa has application prospect in control of insect, especially will be described beautiful
The botanical attractant of aphid chalcid fly is used to prepare or directly as control of insect medicament.One of which application example is, will be described
Attractant be dissolved in paraffin oil, be made and lure agent solution, the weight ratio of the attractant and paraffin oil is 1-10：1.It is described
Lure agent solution to be added drop-wise in lure to form the release with slow releasing function.Agent solution is lured described in each lure
Dripping quantity be 1 gram -8 grams.The lure is sponge or fiberboard.
The present invention has an advantageous effect in that compared with prior art：
Compared with prior art, attractant preparation method of the present invention is simple, easy to use, improves biological control effect.With
Techniques For Chemical Control is compared, and dosage can be reduced, and effectively reduces the pesticide residue in ecological environmental pollution and crops.
Specific implementation mode
Below in conjunction with example, the present invention is described in further detail
Wherein, for examination Encarsia formosa bee card, lure is bought in Beijing sources Ge Ruibi Science and Technology Ltd.
Embodiment 1：
1) by E)-β-ocimenums, beta-myrcene, gaultherolin, β-carypohyllene is according to 1:1:1:1 mass ratio mixing
2) by 1) mixed component and paraffin oil according to weight 1：1 ratio mixing, obtains luring agent solution.
3) it lures agent solution to be added drop-wise in lure to form the release with slow releasing function by what is 2) obtained, in each lure
It is 1 gram of to lure the dripping quantity of agent solution
Embodiment 2：
1) by E)-β-ocimenums, beta-myrcene, gaultherolin, β-carypohyllene is according to 4:3:2:1 mass ratio mixing
2) by 1) mixed component and paraffin oil according to weight 10：1 ratio mixing, obtains luring agent solution.
3) it lures agent solution to be added drop-wise in lure to form the release with slow releasing function by what is 2) obtained, in each lure
It is 8 grams to lure the dripping quantity of agent solution.
Embodiment 2：
The Encarsia formosa botanical attractant that embodiment 1 is prepared carries out real in metering university of China glasshouse
It tests.
1) two long 10m, wide 8m, high 1.8m, the solarium of 60 mesh fly net of outer cover, to two nets are established in glasshouse
Indoor transplanting tomato seedling.
2) after tomato transplantation of seedlings, Bemisia tabaci progress indoor propagation is accessed in six solariums.Wait for Bemisia tabaci mass propagation 20
After it, wherein hanging Encarsia formosa bee in three solariums blocks and accesses the release that embodiment 1 is prepared, as processing group；Separately
Outer three solariums only hang Encarsia formosa bee card, do not access release, as a control group.3) cigarette of each solarium before bee card is hung in investigation
Aleyrodid quantity, statistics put 27 days Bemisia tabaci quantity after bee.Using 5 point samplings, every 2 plants of tomato seedling of investigation, every plant random
Each 1 of upper, middle and lower portion blade is investigated, blade is gently stirred, counts Bemisia tabaci adults and nymph quantity, and calculate mean univalent functions worm
Amount and Revision insect recluced rate.Revision insect recluced rate (%)=(pest number after number of pest-release before release) preceding number of pest of/release ×
100。
4) gained test data carries out variance analysis (the new multipoles of Duncan ' s with Microsoft Excel and DPS software
Poor method).
The effect of beautiful aphid control Bemisia tabaci is discharged in solarium.
As a result with analysis：From 1 result of table as it can be seen that the solariums Fang Feng of release of the access containing plant source derivant are processing
Group, Bemisia tabaci list leaf worm amount puts adult 45.1 ± 12.3 before bee in processing group, and nymph 46.6 ± 10.7 subtracts after putting bee 27 days
Adult 10.2 ± 3.1, nymph 9.4 ± 4.0 are arrived less；Revision insect recluced rate reaches 78.6 ± 5.6%, control group Bemisia tabaci list leaf
Worm amount puts before bee 40.2 ± 10.5, and nymph 58.1 ± 13.3, after putting bee 27 days, single leaf adult quantity is 23.7 ± 7.6,
Single leaf nymph 20.1 ± 7.3, Revision insect recluced rate reaches 55.4 ± 5.1%.Through statistical analysis, the two significant difference (P=
0.007).As it can be seen that the release containing plant source derivant is arranged in solarium prevents Bemisia tabaci significant effect to Encarsia formosa.
Specific experiment data are shown in Table 1
Table 1, which accesses release after discharging Encarsia formosa and do not access bemisia tabaci number amount in release solarium, to be changed
Embodiment 3
1) two long 10m, wide 8m, high 1.8m, the solarium of 60 mesh fly net of outer cover, to two nets are established in glasshouse
Indoor transplanting tomato seedling.
2) after tomato transplantation of seedlings, Bemisia tabaci progress indoor propagation is accessed in the solariums Liang Ge.After Bemisia tabaci mass propagation,
The solariums Liang Ge hang Encarsia formosa bee card and are prevented, and extension Encarsia formosa bee, which blocks and accesses embodiment 1, in a solarium is prepared
Release, another solarium only hang Encarsia formosa bee card, do not access release, as a contrast.
3) it puts bee point to be arranged in the middle part of solarium, centered on putting bee point, at 2,4,6,8 meters of distance center point east-west direction
It makes marks, puts after bee 3 days, acquire blade, observe parasitic rate, analyze the relationship between diffusion length and parasitic rate.
Parasitic rate (%)=(if by Parasitic Nymph number/total borer population) × 100%.
As a result with analysis：Control group is the experimental results showed that (table 2), Encarsia formosa are started to spread to east-west direction from central point
Distance is remoter, lower to the parasitic rate of Bemisia tabaci.The mean parasitized rates of three reprocessings by central point westwards 2,4,6,8 successively
It is 85.12%, 80.94%, 30.12%, 20.46%, 15.24%；2,4,6,8 it is followed successively by 85.12% eastwards by central point,
77.36%, 25.23%, 14.78% and 8.27%；
The experimental group of release is accessed the experimental results showed that (table 2), Encarsia formosa are started to expand to east-west direction from central point
Scattered distance is remoter, lower to the parasitic rate of Bemisia tabaci, but parasitic rate remains above control group.The mean parasitized rate of three reprocessings
Westwards 2,4,6,8 it is followed successively by 85.12%, 82.94%, 60.12%, 50.17%, 30.42% by central point；Eastwards by central point
2,4,6,8 it is followed successively by 85.12%, 80.36%, 45.32%, 31.28% and 28.72%；From the above results, it can be seen that Encarsia formosa
Botanical attractant can improve the diffusivity of Encarsia formosa, improve its parasitic rate.
2 Encarsia formosa diffusion length of table and parasitic rate statistics
To sum up, the present invention can effectively reduce the Revision insect recluced rate of Bemisia tabaci, improve Encarsia formosa diffusivity and
Parasitic rate improves the effect of biological control.
Claims (7)
1. a kind of botanical attractant of Encarsia formosa, which is characterized in that including following component：(E)-β-ocimenums, β-bay
Alkene, gaultherolin, β-carypohyllene；(E)-β-ocimenums, beta-myrcene, gaultherolin, the weight ratio of β-carypohyllene
For (1-4):(1-3):(1-2):1.
2. a kind of botanical attractant of Encarsia formosa according to claim 1, which is characterized in that the Encarsia formosa
Botanical attractant further include account for total constituent mass 1-5% antioxidant and account for total constituent mass 1-5% uvioresistant it is steady
Determine agent.
3. application of the botanical attractant of Encarsia formosa as claimed in claim 1 or 2 in control of insect.
4. application according to claim 3, which is characterized in that the botanical attractant of the Encarsia formosa is dissolved in stone
It in wax oil, is made and lures agent solution, the weight ratio of the attractant and paraffin oil is (1-10)：1.
5. application according to claim 4, which is characterized in that described lures agent solution to be added drop-wise to slow releasing function
In lure, that is, form the release of attractant slow release.
6. application according to claim 5, which is characterized in that the dripping quantity of agent solution of luring is in each lure
1 gram -8 grams.
7. application according to claim 5 or 6, which is characterized in that the lure is sponge or fiberboard.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810541730.0A CN108770848A (en) | 2018-05-30 | 2018-05-30 | A kind of botanical attractant of Encarsia formosa and its application |
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| CN201810541730.0A CN108770848A (en) | 2018-05-30 | 2018-05-30 | A kind of botanical attractant of Encarsia formosa and its application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109874807A (en) * | 2019-03-25 | 2019-06-14 | 陕西省生物农业研究所 | It is a kind of for preventing and treating the Bemisia tabaci extract preparation process of TYLCV infection insect |
| CN110583645A (en) * | 2019-09-16 | 2019-12-20 | 中国计量大学 | Harmonia axyridis plant source attractant and application thereof |
| CN113508711A (en) * | 2021-07-21 | 2021-10-19 | 湖南农业大学 | Method for applying beta-ocimene in open space |
| CN115633683A (en) * | 2022-10-14 | 2023-01-24 | 虫捕头(苏州)生物科技有限公司 | Apple-spotted turnip plant source attractant active component and attractant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874807A (en) * | 2019-03-25 | 2019-06-14 | 陕西省生物农业研究所 | It is a kind of for preventing and treating the Bemisia tabaci extract preparation process of TYLCV infection insect |
| CN109874807B (en) * | 2019-03-25 | 2021-02-09 | 陕西省生物农业研究所 | Preparation process of bemisia tabaci extract for preventing and treating TYLCV (TYLCV-transmitted viral insects) |
| CN110583645A (en) * | 2019-09-16 | 2019-12-20 | 中国计量大学 | Harmonia axyridis plant source attractant and application thereof |
| CN113508711A (en) * | 2021-07-21 | 2021-10-19 | 湖南农业大学 | Method for applying beta-ocimene in open space |
| CN113508711B (en) * | 2021-07-21 | 2024-01-09 | 刘春林 | Method for applying beta-ocimene in open space |
| CN115633683A (en) * | 2022-10-14 | 2023-01-24 | 虫捕头(苏州)生物科技有限公司 | Apple-spotted turnip plant source attractant active component and attractant |
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Application publication date: 20181109 |
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