CN1242669C - Method for trapping Empoasca vitis Gothe imago/nymph and Aleurocanthus spiniferus imago - Google Patents
Method for trapping Empoasca vitis Gothe imago/nymph and Aleurocanthus spiniferus imago Download PDFInfo
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- CN1242669C CN1242669C CN 200410018062 CN200410018062A CN1242669C CN 1242669 C CN1242669 C CN 1242669C CN 200410018062 CN200410018062 CN 200410018062 CN 200410018062 A CN200410018062 A CN 200410018062A CN 1242669 C CN1242669 C CN 1242669C
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Abstract
The present invention discloses a trap method for small green leaf hopper imagines and nymphs with artificial eyes and spiny whitefly imagines in tea gardens, which comprises the steps: 1) an adsorption method is used for separating and identifying nine volatile objects from a sturdy tea twig; 2) a biological measure method is used for determining three volatile components with attracting activity; 3) the three active components are respectively prepared into taste source objects with the concentration of 0.01 to 20 mu g/ml, and attractant is prepared by the ratio of 1: 0.2 to 1.5: 0.1 to 1; 4) an attracting test in tea garden areas is used for determining parrot green, bud green and jasmine yellow, which have the attracting effect on leaf hoppers and whiteflies, from ten colors; 5) the bud green and the jasmine yellow are combined with the attractant to prepare a trap device for trapping leaf hoppers and the whiteflies; 6) the trap device is placed in the field for trapping the leaf hoppers and the whiteflies. The present invention has the advantages of no pollution, high efficiency, stable performance, convenient transportation, convenient use and low cost, and is suitable for producing present organic tea and nuisance free tea, many leaf hopper imagines and nymphs and whitefly imagines can be trapped in a test area for 1 to 2 days, and the chemical pesticide application is derated.
Description
Technical field
The present invention relates to a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation.
Background technology
China's tea place area, output and export volume apportion the first in the world, second and the 3rd.False eye leafhopper Empoasca vitis G the and Aleurocanthus spiniferus Aleurocanthus spiniferus (Quaintance) are important tea tree insect.False eye leafhopper belongs to Homoptera Homoptera Cicadellidae Cicadellidae, is a kind of polyphagy insect on weeds and the woody plant originally.50～sixties of 20th century, China's large tracts of land kind of tea of reclaiming wasteland, this leafhopper is transferred on the tea tree from host plants such as weeds and shrubs, progressively is evolved into sociales.It is distributed widely in China tea district and neighbouring country tea districts such as Vietnam and Burma.Generation overlap is from China Qinling Mountains, the area, Huaihe River is to Hainan Island, about 7～15 generations of annual breeding.Form 2 insect population peaks in annual late May～early July, 9～November.Inhale the tender tip with one-tenth, nymph thorn, lay eggs in the tender stem of the tea tip.Wilting, curling, the burnt limit of the bud-leaf of being injured, come off, the leaf look reddish brown, and shape is as burning.Because this leafhopper fertility is strong, lacks effective natural enemy, is the No.1 tea tree insect of China in decades, mainly relies on chemical control always.The annual chemical control in many tea district more than 10 time during the summer autumn tea, was executed medicine 1 time in per 7～10 days, caused serious residue of pesticide, and caused this leafhopper and produce obvious pesticide resistance
[1]
Aleurocanthus spiniferus belongs to Homoptera Homoptera Aleyrodidae Aleyrodidae, and varying degree abroad causes harm in state tea places such as Japan and India.Its habit covers, and the individuality more than 80% is distributed in the tea clump, lower floor becomes leaf back.And generation overlap is difficult to radical cure.Early 1960s, the beginning of the seventies, the beginning of the eighties, the end of the eighties to the beginning of the nineties once broke out in tea district intermittences such as China middle and lower reach of Yangtze River, south China and Hainan, caused that sooty mold is very popular, and large-area tea place is as dark as a stack of black cats.The mechanism that not clear and definite so far its population wildness is caused disaster and collapsed.The insect population of Zhejiang and Jiangxi Anhui this aleyrodid of Cha Qu is bigger since 2000.The wax layer of its larva and pupal cell table is thicker, and tea Cong Peng face sprays medicine fast and it killed and wounded very little, all is high concentration heavy dose of ground dispenser when the big generation of this aleyrodid usually.Though can control insect pest, very heavy to killing and wounding of natural enemy.The loss of Tea Production then is also very serious
[2]
Have an appointment at present 10000 hectares organic tea garden of China, this class tea place does not allow to use chemical fertilizer, agricultural chemicals, but insect pest still seriously takes place, and especially false eye leafhopper does not have effective prophylactico-therapeutic measures.Except that organic tea garden, China also has 1,000,000 hectares of nuisanceless tea places.The Ministry of Agriculture of country regulation pollution-free tea will be the minimum requirements and the market access requirements of China's Tea Production, and the application of chemical pesticide will gradually reduce.Given this, must expand the new non-environmental pollution control approach of these two kinds of insects.
Insect chemistry information substance environmentally safe, using dosage small (mg～μ g level), active strong.Since the seventies in 20th century, insect sex pheromone is always as one of important nuisanceless measure, and thousands of mu of ground are used.Since the nineties, the chemical communication between host plant-insect-natural enemy three trophic levels becomes the research focus, and the smell of host plant is attracted the insect location, stimulated and get food.These new discoveries evoke researchers' interest greatly, and they long for it is put to control the worm practice
[3]Insect generally all has phototaxis
[4], continuing to use the light trap of decades, yellow plate, to lure technology such as aphid be exactly to have utilized these habits of insect.Wang Yong etc. (1991)
[5]Report khaki ticker tape has seduction to the tea geometrid larva.Xiao Sunv (1997)
[6]Studied the attracting action of white, blue, yellow, yellowish green, blackish green 5 kinds of colour tables to tea lesser leafhopper Jacobiasca formosana Paoli and Aleurocanthus spiniferus Aleurocanthus spiniferus (Quaintance), find the effective of the sticking paper trapping of yellow green leafhopper, yellow paper is effective to Aleurocanthus spiniferus.Yet this tea lesser leafhopper is not the Empoasca vitis G that we study; If " chromatogram " according to the writing of the compiling publication committee (1957) of the Chinese Academy of Sciences
[7], yellow green or yellow all also can be subdivided into many types; And this article does not relate to the attracting action of tea shoot volatile.The present invention is with color and information substance combine trapping leafhopper and aleyrodid.The deduction and exemption dispenser, it is residual to reduce the tealeaves farming, promotes the organic tea and the nuisanceless tealeaves production of China.
List of references
[1] Zhu Junqing. the prediction of smaller green leaf hopper occurrence tendency. tea science, 1986,6 (2): 41～46
[2] Han Baoyu, Cui Lin. tea place Aleurocanthus spiniferus nature Population life-table. Acta Ecologica Sinica, 2003,23 (9): 1781～1790
[3]Pickett?J.A.，Wadhams?L.J.，Woodcock?C.M.The?chemical?ecology?ofaphids.Ann.Rev.Entomol.，1992，37：67～90
[4] Chen Ningsheng. visual communication. see: Chinese agriculture encyclopedia insect roller editorial board compiles, Chinese agriculture encyclopedia insect roller. the agriculture .1990.362 of publishing house～363
[5] Wang Yong, Zhang Hangu, Zou Yunding. ticker tape lures the collection Study on Efficiency to the tea geometrid larva. plant protection journal, 1991,18 (2): 177～180
[6] Xiao Sunv. the effect research of coloured sticking paper trapping pests in the tea place. Tea Industry research in Taiwan is reported, and 1997,16:51～60
[7] compile and edit Chinese Academy of Sciences's compiling publication committee noun chamber. chromatogram. and Beijing: the .1957 of Science Press
Summary of the invention
The purpose of this invention is to provide a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation.
The step of method is: 1) adopt absorption method isolation identification from the healthy and strong tea tip to go out 9 kinds of volatile matters: anti--the 2-hexenoic aldehyde, ocimene, suitable-3-hexene ethyl ester, suitable-blatter alcohol, butyric acid-3-hexene ester, linalool, the 1-octanol, geraniol and indoles, 2) adopt bioassay method to determine suitable-blatter alcohol, instead-2-hexenoic aldehyde and linalool are the volatile component with attractive activity, 3) with suitable-blatter alcohol, instead-2-hexenoic aldehyde and linalool be made into the odor source that concentration is 0.01-20 μ g/ml respectively, again three kinds of odor sources are pressed 1: 0.2-1.5: 0.1-1 assembly, be mixed with attractant, 4) it is green to adopt sub-district, tea place trapping experiment to determine leafhopper and aleyrodid are had the parrot of seduction from 10 kinds of colors, green and the yellow 3 kinds of colors of jasmine of bud, 5) green and the yellow 2 kinds of colors of jasmine and suitable-blatter alcohol with bud, instead-2-hexenoic aldehyde and the combination of linalool attractant, make the trapper of trapping leafhopper and aleyrodid, 6) trapper is put into field trapping leafhopper and aleyrodid.
Advantage of the present invention:
1) pollution-free, be adapted to current organic tea and nuisanceless tealeaves production;
2) efficient height, stable performance, just many leafhopper one-tenth, nymph and adult whitefly trapping, deduction and exemption in the test region can be used chemical pesticide in 1～2 day: respect booth organic tea garden mountain in Anhui and trap false eye leafhopper with this method, reduce insect population 54.7%, trap Aleurocanthus spiniferus in the nuisanceless tea place of Anhui Ma Gushan with this method, on average the Aleurocanthus spiniferus imago of each trapper trapping is up to thousands of heads;
3) be convenient to transportation, easy to use;
4) cost is low.
Description of drawings
Accompanying drawing is the structural representation of trapper of the present invention.
Embodiment
1, adopt absorption method isolation identification from the healthy and strong tea tip to go out 9 kinds of volatile matters: anti--the 2-hexenoic aldehyde, ocimene, suitable-3-hexene ethyl ester, suitable-blatter alcohol, butyric acid-3-hexene ester, linalool, 1-octanol, geraniol and indoles
Make two and have the glass column that bore is 6～10 millimeters tiny openings, hollow, 4.5～5.5 liters of volumes, import connects compressed air bottle, and outlet connects Tenax TA adsorption column, vavuum pump successively.Before the adsorbing volatilizing thing, at 270～280 ℃, logical nitrogen activates 4～8 hours with Tenax TA post.Add the healthy and strong tea tip of 190～210 grams in glass container, be blown into air, bleed behind adsorption column simultaneously, control flow with flowmeter before import, behind the Tenax TA post, the inlet, outlet flow all is 300 ml/min.
Take off adsorption column after 11～13 hours,, slowly purge with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component to 15～30 microlitres with heavily steaming ether drip washing.Identified 9 kinds of chemical constituents in the tea shoot volatile: suitable-blatter alcohol, anti--the 2-hexenoic aldehyde, linalool, ocimene, suitable-3-acetate hexene ester, butyric acid-3-hexene ester, 1-octanol, geraniol and indoles, wherein suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool are the big components of content.
2, adopt bioassay method to determine that suitable-blatter alcohol, anti--2-hexenoic aldehyde and linalool are the volatile component with attractive activity
1) adopt " Y " shape olfactometer, Y manages two arms and base portion length is 10cm, and two arm angles are 90 °, connect flavor source bottle (or control bottle), distilled water bottle, activated carbon bottle successively.Bleed with vavuum pump from Y pipe base portion, natural air crosses in arm crossing place of Y pipe successively through the filtration of activated carbon bottle, distilled water bottle humidification, flavor source bottle (or control bottle), enters Y pipe base portion.Stop to bleed, introduce insect from the Y pipe base portion mouth of pipe and bleed, insect will be managed two arm intersections at Y to stop slightly against the wind forward, just selects an arm to enter.
With the n-hexane is solvent, and with 9 kinds of tea shoot volatiles difference such as suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool wiring solution-forming, concentration is 0.01～1 μ g/ml, as odor source, is contrast with the n-hexane.Volume with opening is that 2 milliliters of peace a word used in place name bottles are contained 1 milliliter odor source (or n-hexane), place flavor source bottle (or control bottle), introduce false eye leafhopper adult, false eye leafhopper nymph, Aleurocanthus spiniferus imago by head, bleed, statistics is selected the insect number of flavor source and n-hexane, detects the difference between the insect number of the insect number of selecting the flavor source and selection n-hexane with χ 2 methods.
2) find 9 kinds for the examination volatile matter in, the borer population of the suitable-3-hexene ethanol of trend, anti--2-hexenoic aldehyde and linalool in leafhopper adult, leafhopper nymph, the adult whitefly, and the difference between the borer population of trend contrast (n-hexane) reaches significance level (P＜0.05).
3, suitable-blatter alcohol, anti--2-hexenoic aldehyde and linalool are made into the odor source that concentration is 0.01-20 μ g/ml respectively, again three kinds of odor sources are pressed 1: 0.2-1.5: the 0.1-1 assembly is mixed with attractant
With n-hexane is solvent, suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool are made into the odor source that concentration is 0.01-20 μ g/ml respectively, again with three kinds of odor sources in 1: 0.2-1.5: 0.1-1 ratio assembly, the biologicall test of being done through " Y " shape olfactometer shows that attractant effect is stronger than one pack system.The attractant of Here it is false eye leafhopper one-tenth, nymph and Aleurocanthus spiniferus imago.
The rubber tip made from the soaked in absolute ethyl alcohol natural rubber was dried more than 24 hours.Soaked such rubber tip 12～36 hours with attractant again, just made and lured core.
4, adopt sub-district, tea place trapping experiment to determine from 10 kinds of colors leafhopper and aleyrodid are had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine
1) field plot trial determines to have the color of allure
Purchase that bud is green, parrot is green, jasmine is yellow, rape is brightly yellowish, olive is yellowish green, calcedony is green, peacock green, bluish-green, blackish green and snow-white 10 kinds of ticker tapes, every kind of color is all made the rectangular two-sided colour table of 10 block lengths, colourless insect glue is smeared on the two sides of colour table equably, places tea place trapping false eye leafhopper and aleyrodid.The result show that parrot is green, bud is green with jasmine yellow to leafhopper become, nymph and adult whitefly allure be stronger.
2) parameter of 3 kinds of strong allure colors of mensuration
The color colour difference meter (color difference meter) that uses Hong Kong Minolta company to produce, model C R-331C, the colour space is selected absolute measurement mode (L for use
*a
*b
*The aberration system).Record:
The green L 69.00 of parrot (67～70) a-43.57 (40～-47) b+44.27 (+40～+ 47)
The green L 75.42 of bud (73～75) a-40.57 (40～-47) b+47.43 (+44～+ 50)
Yellow L 86.96 (84～90) a-5.84 (2～-9) b+64.77 (+61～+ 68) of jasmine
Wherein the 1st numerical value L represents brightness; A, b are chromaticity coordinates, and the 2nd numerical value "+" is represented red composition, and "-" represents green composition; The 3rd numerical value "+" expression Huang Cheng part, "-" represents blue composition.
5, the trapper of trapping leafhopper and aleyrodid is made in green and the yellow 2 kinds of colors of jasmine and suitable-blatter alcohol, anti--2-hexenoic aldehyde and the combination of linalool attractant with bud
1) it is described that attractant is exactly " embodiment 3 ", easy attractant is stated from lure on the core.
2) trapper of trapping false eye leafhopper imago/nymph is that the green colour table of bud adds and lures core.Trapping Aleurocanthus spiniferus imago trapper is that the yellow plate of jasmine adds and lures core.
6, trapper is put into field trapping leafhopper and aleyrodid
False eye leafhopper trapper or Aleurocanthus spiniferus trapper are positioned in the tea place, and the trapper bottom is concordant with the tea tip, and the distance between the trapper is 4～8 meters.
7, embodiment
Embodiment 1: adopt absorption method isolation identification from the healthy and strong tea tip to go out 9 kinds of volatile matters: anti--the 2-hexenoic aldehyde, ocimene, suitable-3-hexene ethyl ester, suitable-blatter alcohol, butyric acid-3-hexene ester, linalool, 1-octanol, geraniol and indoles
Make two and have the glass column that bore is 7 millimeters tiny openings, hollow, 5 liters of volumes, import connects compressed air bottle, and outlet connects Tenax TA adsorption column, vavuum pump successively.Before the adsorbing volatilizing thing, the TenaxTA post is activated 4 hours at 275 ℃ of logical nitrogen.Add the healthy and strong tea tip of 200 grams in glass container, be blown into air, bleed behind adsorption column simultaneously, control flow with flowmeter before import, behind the Tenax TA post, the inlet, outlet flow all is 300 ml/min.
Take off adsorption column after 12 hours,, slowly purge with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component to 20 microlitres with heavily steaming ether drip washing.Identified 9 kinds of chemical constituents in the tea shoot volatile: suitable-blatter alcohol, anti--the 2-hexenoic aldehyde, linalool, ocimene, suitable-3-acetate hexene ester, butyric acid-3-hexene ester, 1-octanol, geraniol and indoles, wherein suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool are the big components of content.
Embodiment 2: adopt bioassay method to determine that suitable-blatter alcohol, anti--2-hexenoic aldehyde and linalool are the volatile component with attractive activity
Described according to embodiment 2, with the n-hexane is solvent, with 9 kinds of tea shoot volatiles difference such as suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool wiring solution-forming, concentration is 1 μ g/ml, as odor source, with the n-hexane is contrast, utilizes Y shape olfactometer to measure the trend quantity in leafhopper adult, leafhopper nymph, the right source of adult whitefly.Discovery 9 kinds for the examination volatile matter in, the borer population of the suitable-3-hexene ethanol of trend, anti--2-hexenoic aldehyde and linalool in leafhopper adult, leafhopper nymph, the adult whitefly, and the difference between the borer population of trend contrast (n-hexane) reaches significance level (P＜0.05).
Embodiment 3: suitable-blatter alcohol, anti--2-hexenoic aldehyde and linalool are made into the odor source that concentration is 0.01-20 μ g/ml respectively, and again three kinds of odor sources are pressed 1: 0.2-1.5: the 0.1-1 assembly is mixed with attractant
With n-hexane is solvent, suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool are made into the odor source that concentration is 1 μ g/ml respectively, again with three kinds of odor sources in ratio assembly in 1: 1: 1, the biologicall test of being done through " Y " shape olfactometer shows that attractant effect is stronger than one pack system.The attractant of Here it is false eye leafhopper one-tenth, nymph and Aleurocanthus spiniferus imago.
The rubber tip made from the soaked in absolute ethyl alcohol natural rubber 24 hours is dried.Soaked such rubber tip 24 hours with attractant again, just made and lured core.
Embodiment 4: adopt sub-district, tea place trapping experiment to determine from 10 kinds of colors leafhopper and aleyrodid are had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine
Described by embodiment 4, make colored steel.On October 20th, 2002 was lured leafhopper in the Tea Inst., Chinese Academy of Agricultural Sciences tea place.The average every block of plate trapping of above-mentioned 10 kinds of colour tables leafhopper becomes, nymph is respectively: 58 ± 16,53 ± 21,54 ± 18,50 ± 24,48 ± 19,42 ± 21,34 ± 24,22 ± 12, and 18 ± 10 and 15 ± 10.On July 2nd, 2002, the fiber crops Gu Shanchachang of Anhui Province test, above-mentioned 10 kinds of colour tables trapping Aleurocanthus spiniferus imago: above-mentioned 10 kinds of colour tables trapping adult whitefly is respectively: 246 ± 89,238 ± 78,267 ± 76,251 ± 84,235 ± 89,214 ± 91,204 ± 84,187 ± 62,148 ± 59 and 142 ± 76.Analysis-by-synthesis, think that parrot is green, bud is green with jasmine yellow to leafhopper become, nymph and adult whitefly allure be stronger.
Embodiment 5: green and the yellow 2 kinds of colors of jasmine and suitable-blatter alcohol, anti--2-hexenoic aldehyde and the combination of linalool attractant with bud, make the trapper of trapping leafhopper and aleyrodid
Described by embodiment 5, with concentration be 1 μ g/ml suitable-blatter alcohol, anti--2-hexenoic aldehyde and linalool make attractant in 1: 1: 1 ratio.With green viscose glue colour table of bud and attractant combination, make the false eye leafhopper trapper again; With yellow viscose glue colour table of jasmine and attractant combination, make the trapper of aleyrodid
Embodiment 6: trapper is put into field trapping leafhopper and aleyrodid
1) trapping false eye leafhopper imago/nymph
Select the tealeaves company tea place of being bestowed by heaven, Ningbo City, Zhejiang Province, 30 mu of areas, on September 21st, 2003, with its central area for experimental field.Get the green plates of 10 buds and add and lure core, lure the attractant that is loaded with the trapping leafhopper on the core; Other gets the green plate of 10 buds and adds the core that lures of not planting attractant, and this lures core to be soaked by n-hexane.20 blocks of plates are placed in the tea place spacing 5m * 5m at random.September 23 the tea attractant effect.
Leafhopper imago/nymph quantity variance is lured in table 1 leafhopper trapper and contrast
(Ningbo City, Zhejiang Province be bestowed by heaven tealeaves company, on September 23rd, 2003)
Average every attractant colour table lure 195.8 ± 67.5 of leafhopper,
Average every contrast colour table lure 117.1 ± 44.4 of leafhopper,
The t test Analysis shows that the difference between attractant colour table and contrast colour table reaches significance level (P＜0.05).
2) trapping Aleurocanthus spiniferus imago
The nuisanceless tea place of selecting Anhui Province fiber crops Gu Shanchachang is for experimental field, 30 mu of areas.In late June, 2003～early July is the Aleurocanthus spiniferus emergence Sheng phase in the 1st generation.Checking in average every leaf aleyrodid pupa density with five point samplings is 3.1, super experience index for control (2.0 in average every leaf worm mouth).July 2 will experimental field be divided into two parts, and a part does not deal with, and be blank; Another part is provided with trapper, and trapper density is 4m * 4m.The jasmine Huang, bud is green and the green trapper of parrot is respectively 51,46 and 50.Add up the adult whitefly quantity of every trapper trapping:
1109.0 ± 729.0 of adult whiteflies are traped in jasmine Huangping all each trapper.
712.5 ± 506.0 of green average each trapper trapping adult whiteflies of bud.
594.2 ± 461.4 of green average each trapper trapping adult whiteflies of parrot.
Claims (9)
1, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation, the step that it is characterized in that method is: 1) adopt absorption method isolation identification from the healthy and strong tea tip to go out 9 kinds of volatile matters: anti--the 2-hexenoic aldehyde, ocimene, suitable-3-hexene ethyl ester, suitable-blatter alcohol, butyric acid-3-hexene ester, linalool, the 1-octanol, geraniol and indoles, 2) adopt bioassay method to determine suitable-blatter alcohol, instead-2-hexenoic aldehyde and linalool are the volatile component with attractive activity, 3) with suitable-blatter alcohol, instead-2-hexenoic aldehyde and linalool be made into the odor source that concentration is 0.01-20 μ g/ml respectively, again three kinds of odor sources are pressed 1: 0.2-1.5: 0.1-1 assembly, be mixed with attractant, 4) adopt sub-district, tea place trapping experiment green from bud, parrot is green, the jasmine Huang, rape is brightly yellowish, olive is yellowish green, calcedony is green, peacock green, bluish-green, the parrot of determining leafhopper and aleyrodid are had seduction in blackish green and snow-white 10 kinds of colors is green, green and the yellow 3 kinds of colors of jasmine of bud, 5) green and the yellow 2 kinds of colors of jasmine and suitable-blatter alcohol with bud, instead-2-hexenoic aldehyde and the combination of linalool attractant, make the trapper of trapping leafhopper and aleyrodid, 6) trapper is put into field trapping leafhopper and aleyrodid.
2, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 1, it is characterized in that said employing absorption method isolation identification from the healthy and strong tea tip go out 9 kinds of volatile matters anti--the 2-hexenoic aldehyde, ocimene, suitable-3-hexene ethyl ester, suitable-blatter alcohol, butyric acid-3-hexene ester, linalool, the 1-octanol, geraniol and indoles: it is 6～10 millimeters tiny openings that making two has bore, the glass column of hollow, 4.5～5.5 liters of volumes, import connects compressed air bottle, outlet connects Tenax TA adsorption column successively, vavuum pump, before the adsorbing volatilizing thing, Tenax TA post is activated 4～8 hours at 270～280 ℃ of logical nitrogen, in glass container, add the healthy and strong tea tip of 190～210 grams, be blown into air, behind adsorption column, bleed simultaneously, before import, control flow with flowmeter behind the Tenax TA post, take off adsorption column after 11～13 hours, with heavily steaming ether drip washing, slowly purge to 15～30 microlitres with nitrogen again, draw 1 μ l and inject the GC-MS detection, cooperate standard specimen to identify component.
3, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 1, it is characterized in that said employing bioassay method determines suitable-blatter alcohol, instead-2-hexenoic aldehyde and linalool are the volatile component with attractive activity: with " Y " shape olfactometer, Y manages two arms and base portion length is 9～11 centimetres, two arm angles are 90～110 °, connect flavor source bottle or control bottle successively, distilled water bottle, the activated carbon bottle, bleed with vavuum pump from Y pipe base portion, natural air is successively through active carbon filtration, the distilled water bottle humidification, flavor source bottle or control bottle, cross in arm crossing place of Y pipe, enter Y pipe base portion, stop to bleed, introducing insect from Y pipe base bleeds again, insect will stop in arm crossing place of Y pipe against the wind forward slightly, just selects an arm to enter; With the n-hexane is solvent, with suitable-blatter alcohol, instead-2-hexenoic aldehyde and 14 kinds of tea shoot volatiles difference of linalool wiring solution-forming, concentration is 0.01～20 μ g/ml, as odor source, with the n-hexane is contrast, 2 milliliters of odor sources of pacifying 1 milliliter of a word used in place name bottles Sheng with opening place flavor source bottle, the n-hexane of containing 1 milliliter places control bottle, introduce the leafhopper imago/nymph by head, adult whitefly, bleed, counting is selected the insect number in flavor source and is selected the insect number of n-hexane, detect the difference of the two with χ 2 methods, find the leafhopper adult, the leafhopper nymph, suitable-the blatter alcohol of adult whitefly trend, instead-borer population of 2-hexenoic aldehyde and linalool, and the difference between the borer population of trend contrast is P＜0.05.
4, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 1, it is characterized in that the said attractant that is mixed with: be solvent 1) with n-hexane, suitable-3-hexene ethanol, anti--2-hexenoic aldehyde and linalool are made into the odor source that concentration is 0.01-20 μ g/ml respectively, again three kinds of odor sources are pressed 1: 0.2-1.5: 0.1-1 assembly; 2) rubber tip made from the soaked in absolute ethyl alcohol natural rubber was dried more than 24 hours, soaked such rubber tip 12～36 hours with attractant again, had just made and had lured core.
5, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 1, it is characterized in that sub-district, said employing tea place trapping experiment determines from 10 kinds of colors leafhopper and aleyrodid are had that the parrot of seduction is green, bud is green and the yellow 3 kinds of colors of jasmine: in the tea place, do small plot experiment, from 10 kinds of colors, select 3 kinds of colors, measure the parameter of these 3 kinds of colors with seduction.
6, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 5, it is characterized in that said 3 kinds of the colors of from 10 kinds of colors, selecting: bud is green, parrot is green, jasmine is yellow, rape is brightly yellowish, olive is yellowish green, calcedony is green, peacock green, bluish-green, blackish green and snow-white 10 kinds of ticker tapes with seduction, make rectangular two-sided colour table, be coated with transparent insect glue on the colour table two sides, in insect population peak period trapping leafhopper imago/nymph and adult whitefly, select that parrot is green, bud is green and jasmine yellow 3 has the color of seduction.
7, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 5, the parameter that it is characterized in that these 3 kinds of colors of said mensuration: the color colour difference meter that uses Hong Kong Minolta company to produce, model C R-331C, the colour space is selected the absolute measurement mode for use, records:
The green L67 of parrot～70 a-40～-47 b+40～+ 47
The green L73 of bud～75 a-40～-47 b+44～+ 50
The yellow L84 of jasmine～90 a-2～-9 b+61～+ 68
Wherein the 1st numerical value L represents brightness; A, b are chromaticity coordinates, and the 2nd numerical value "+" is represented red composition, and "-" represents green composition; The 3rd numerical value "+" expression Huang Cheng part, "-" represents blue composition.
8, a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation according to claim 1, it is characterized in that said green and the yellow 2 kinds of colors of jasmine and suitable-blatter alcohol, anti--2-hexenoic aldehyde and the combination of linalool attractant with bud, make the trapper of trapping leafhopper and aleyrodid: the trapper of trapping false eye leafhopper imago/nymph is that the green colour table of bud adds the core that lures that is loaded with the described attractant of claim 4, and trapping Aleurocanthus spiniferus imago trapper is the core that lures that the yellow plate of jasmine adds the attractant that is loaded with the described attractant of claim 4.
9, according to claim 1 or 8 described a kind of trapping method of adult tea green leafhopper and adult aleurocanthus spiniferus in tea plantation, it is characterized in that said trapper being put into field trapping leafhopper and aleyrodid: in false eye leafhopper or Aleurocanthus spiniferus peak period, false eye leafhopper trapper or Aleurocanthus spiniferus trapper are positioned in the tea place, the trapper bottom is concordant with the tea tip, and the distance between the trapper is 4～8 meters.
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