CN102144638B - Anagrus nilaparvatae attractant - Google Patents
Anagrus nilaparvatae attractant Download PDFInfo
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- CN102144638B CN102144638B CN 201110072378 CN201110072378A CN102144638B CN 102144638 B CN102144638 B CN 102144638B CN 201110072378 CN201110072378 CN 201110072378 CN 201110072378 A CN201110072378 A CN 201110072378A CN 102144638 B CN102144638 B CN 102144638B
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Abstract
The invention discloses an anagrus nilaparvatae attractant. The anagrus nilaparvatae attractant comprises the following components in the weight ratio of gaultherolin to cis-3-hexenoicaldehyde to linalool to cis-3-hexenylacetate to liquid paraffin to 2,6-ditertiary butyl p-cresol to 2-hydroxy-4-methoxy benzophenone of 0.01-0.10:0.01-0.10:0.01-0.10:0.01-0.10:0.2-2:0.01-0.1:0.01-0.1. The anagrus nilaparvatae attractant has induction effect on female adult of the important natural enemy, namely the anagrus nilaparvatae, of rice planthopper at egg stage, so that the number of the anagrus nilaparvatae population is reduced and damage to rice plants is reduced. The anagrus nilaparvatae attractant has the characteristics of low cost, high efficiency, environmental friendliness, safety to people and livestock and the like.
Description
Technical field
The present invention relates to a kind of natural enemy attractant that can effectively control rice fulgorid adult quantity, particularly relate to a kind of natural enemy attractant that utilizes host plant volatile that insect pest induces that the population quantity of rice fulgorid is controlled.
Background technology
Rice fulgorid is one of most important insect on China staple food crop paddy rice, wherein with white-backed planthopper
Sogatella furciferaAnd brown planthopper
Nilaparvata lugens(St l) harm is the heaviest.As to its in addition in time control, break out and to cause very serious loss to China's rice yield when serious.On the south China the Changjiang river in 2005, the rice fulgorid great outburst appears in most of paddy rice producing region, brings massive losses for national Rice Production, gives a lesson to very deep.Present stage still mainly relies on chemical pesticide in China's Rice Production on to the control of rice fulgorid, natural enemy, contaminated environment yet long-term excessive use chemical pesticide not only kills off the insect pests also can cause the pesticide resistance of insect and the problem such as rampant again.Therefore, developing safely and effectively, biopesticide has become one of important directions of cash control rice fulgorid harm.
Plant can specific release volatile materials after being subject to plant-feed insect harm, and these herbivore-induced volatiles that discharge can be lured the natural enemy of plant-feed insect, thereby make plant reach the purpose of defence.In recent years, existing the experimental results shows that herbivore-induced volatile can lure the natural enemy of plant-feed insect, some researchs identify the main component of herbivore-induced volatile, and have found the crucial active component that the plant-feed insect natural enemy is played attracting action by the Collection and analysis of volatile matter.James etc. (2003) utilize first artificial synthetic herbivore-induced volatile component in the garden of plantation hop these components of investigation to the attracting action of useful insect naturally.Found that, can significantly lure the useful insect of multiple in the rural area or section take MeSA and (Z)-3-hexenyl acetate as the artificial synthetic herbivore-induced volatile component of representative.James(2005) again utilize the herbivore-induced volatile component to carry out field trapping experiment, find that the useful insect of total at least 13 kinds or section can be lured by one or more herbivore-induced volatile components.Wherein, MeSA can lure the multiple beneficial insect.Yu(2008) carry out the epigamic experiment in the cotton field and find, MeSA and (Z)-herbivore-induced volatile components such as 3-hexenyl acetate can be lured multiple natural enemy insect.These field experiment results show that herbivore-induced volatile has important value in the middle of control of insect, particularly in biological control.Due to plant-derived self metabolism of herbivore-induced volatile, therefore having avoided to a certain extent now, chemical pesticide excessively uses all additional hazard that ecotope is brought.The herbivore-induced volatile component has very important potential value to the biological control of following insect.
For rice fulgorid, still do not have so far about strengthening attractant exploitation and the application of its Effectiveness of Natural Enemies.For this reason, special proposition content of the present invention thinks that improving rice fulgorid improvement provides effective method.
Summary of the invention
Problem for prior art exists the object of the present invention is to provide a kind of rice lice tassel chalcid fly attractant.
The objective of the invention is to be achieved through the following technical solutions: a kind of rice lice tassel chalcid fly attractant, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.01-0.10 ︰ 0.01-0.10 ︰ 0.01-0.10 ︰ 0.01-0.10 ︰ 0.2-2 ︰ 0.01-0.1 ︰ 0.01-0.1.
Further, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.02-0.08 ︰ 0.02-0.08 ︰ 0.02-0.08 ︰ 0.02-0.08 ︰ 0.4-1.2 ︰ 0.02-0.08 ︰ 0.02-0.08.
Further, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.03-0.07 ︰ 0.03-0.07 ︰ 0.03-0.07 ︰ 0.03-0.07 ︰ 0.6-1 ︰ 0.03-0.07 ︰ 0.03-0.07.
The invention has the beneficial effects as follows, the present invention can produce attracting action to the female adult of rice fulgorid ovum phase Important Natural Enemy rice lice tassel chalcid fly, thereby reduces the rice fulgorid population quantity, alleviates it to the harm of rice plant.The characteristics such as this attractant has that cost is low, efficient is high, environmentally friendly, person poultry safety.Rice planthopper natural enemy rice lice tassel chalcid fly attractant of the present invention has wide industrialization prospect, because the preparation method is simple, is particularly suitable for using in Rice Non-polluted is produced, and has wide economy, society and ecological benefits.
Description of drawings
Fig. 1 is that attractant is to the attracting action design sketch of rice lice tassel chalcid fly;
Fig. 2 is that attractant uses the rear comparison diagram that affects on field rice fulgorid egg parasitism.
Embodiment
The present invention utilizes artificial synthetic herbivore-induced volatile to make attractant, can produce attracting action to the female adult of rice fulgorid ovum phase Important Natural Enemy rice lice tassel chalcid fly, thereby reduce the rice fulgorid population quantity, alleviates its harm.
Rice lice tassel chalcid fly attractant of the present invention, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.01-0.10 ︰ 0.01-0.10 ︰ 0.01-0.10 ︰ 0.01-0.10 ︰ 0.2-2 ︰ 0.01-0.1 ︰ 0.01-0.1.
Preferably, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.02-0.08 ︰ 0.02-0.08 ︰ 0.02-0.08 ︰ 0.02-0.08 ︰ 0.4-1.2 ︰ 0.02-0.08 ︰ 0.02-0.08.
Best, the component that comprises the following weight proportioning: Shui poplar Suan Jia Zhi ︰ is suitable-and the own Xi Quan of 3-︰ virtue Zhang Chun ︰ is along acetic acid-3-hexene Zhi ︰ Ye Ti Shi La ︰ 2,6-di-t-butyl Dui Jia Fen ︰ ESCALOL 567=0.03-0.07 ︰ 0.03-0.07 ︰ 0.03-0.07 ︰ 0.03-0.07 ︰ 0.6-1 ︰ 0.03-0.07 ︰ 0.03-0.07.
Each compound component of the present invention all belongs to plant metabolites, and preparation steps is simple, and the organic solvent usage amount is little, to operating personnel and Environmental security.
Gaultherolin described in formula, suitable-3-hexenoic aldehyde, linalool and all can be available from SIGMA company along acetic acid-3-hexene ester, but be not limited to this.
Above-mentioned rice planthopper natural enemy rice lice tassel chalcid fly attractant, reasonable mixture ratio can effectively be lured rice planthopper natural enemy rice lice tassel chalcid fly.Rice planthopper natural enemy rice lice tassel chalcid fly attractant of the present invention has been given full play to the effect characteristics of the plant volatile that insect pest induces, and is a kind of rice of rice planthopper natural enemy safely and effectively lice tassel chalcid fly attractant.
Further illustrate the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used for explanation the present invention and be not used in the restriction scope of the invention.
The preparation (weight portion) of embodiment 1 rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.01 part of gaultherolin of preparation, suitable-0.01 part of 3-hexenoic aldehyde, 0.01 part of linalool, suitable acetic acid-0.01 part of 3-hexene ester, 0.2 part of atoleine, 2,0.01 part of 6-BHT, 0.01 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 2: the preparation (weight portion) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.10 part of gaultherolin of preparation, suitable-0.10 part of 3-hexenoic aldehyde, 0.10 part of linalool, suitable acetic acid-0.10 part of 3-hexene ester, 2 parts of atoleines, 2,0.10 part of 6-BHT, 0.10 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 3: the preparation (weight portion) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.02 part of gaultherolin of preparation, suitable-0.02 part of 3-hexenoic aldehyde, 0.02 part of linalool, suitable acetic acid-0.02 part of 3-hexene ester, 0.4 part of atoleine, 2,0.02 part of 6-BHT, 0.02 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 4: the preparation (weight portion) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.08 part of gaultherolin of preparation, suitable-0.08 part of 3-hexenoic aldehyde, 0.08 part of linalool, suitable acetic acid-0.08 part of 3-hexene ester, 1.2 parts of atoleines, 2,0.08 part of 6-BHT, 0.08 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 5: the preparation (weight portion) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.03 part of gaultherolin of preparation, suitable-0.03 part of 3-hexenoic aldehyde, 0.03 part of linalool, suitable acetic acid-0.03 part of 3-hexene ester, 0.6 part of atoleine, 2,0.03 part of 6-BHT, 0.03 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 6: the preparation (weight portion) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
0.05 part of gaultherolin of preparation, suitable-0.05 part of 3-hexenoic aldehyde, 0.05 part of linalool, suitable acetic acid-0.05 part of 3-hexene ester, 1 part of atoleine, 2,0.05 part of 6-BHT, 0.05 part of ESCALOL 567 mixes and namely makes rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 7: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.01 mg, suitable-3-hexenoic aldehyde 0.01mg, linalool 0.01 mg, along acetic acid-3-hexene ester 0.01 mg, atoleine 0.2mg, 2,6-BHT 0.01mg, then ESCALOL 567 0.01mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 8: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.10 mg, suitable-3-hexenoic aldehyde 0.10 mg, linalool 0.10 mg, along acetic acid-3-hexene ester 0.10 mg, atoleine 2mg, 2,6-BHT 0.10mg, then ESCALOL 567 0.10mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 9: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.02 mg, suitable-3-hexenoic aldehyde 0.02 mg, linalool 0.02 mg, along acetic acid-3-hexene ester 0.02 mg, atoleine 0.4mg, 2,6-BHT 0.02mg, then ESCALOL 567 0.02mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 10: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.08 mg, suitable-3-hexenoic aldehyde 0.08 mg, linalool 0.08 mg, along acetic acid-3-hexene ester 0.08 mg, atoleine 1.2mg, 2,6-BHT 0.08mg, then ESCALOL 567 0.08mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 11: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.03 mg, suitable-3-hexenoic aldehyde 0.03 mg, linalool 0.03 mg, along acetic acid-3-hexene ester 0.03 mg, atoleine 0.6mg, 2,6-BHT 0.03mg, then ESCALOL 567 0.03mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 12: the preparation method of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: gaultherolin 0.05 mg, suitable-3-hexenoic aldehyde 0.05 mg, linalool 0.05 mg, along acetic acid-3-hexene ester 0.05 mg, atoleine 1mg, 2,6-BHT 0.05mg, then ESCALOL 567 0.05mg evenly mixes, and namely gets rice planthopper natural enemy rice lice tassel chalcid fly attractant.
Embodiment 13: preparation method's (luring core) of rice planthopper natural enemy rice lice tassel chalcid fly attractant
Take each component by following formula: linalool 0.05mg, atoleine 1mg, BHT 0.05mg, ESCALOL 567 0.05mg evenly mixes.In each cuff type compounded rubber lures core carrier (available from The West Company, Phoenixville, Pa, USA) (before use, soaked 30-40 days, during change ethanol 5-8 time) in analytically pure ethanol, drip the liquid 20-100 μ L that fills a prescription.Airing namely gets and lures core.
Test example 1
The rice planthopper natural enemy rice lice tassel chalcid fly attractant that adopts embodiment 12 to make.Use Y-shaped smell sensing apparatus to measure rice lice tassel chalcid fly to the behavior reaction of this attractant.Two arms of " Y " type olfactometer and straight tube be long 10.0cm all, internal diameter 1.0cm, two 75 ° of arm angles." Y " type two arms are connected by silicone tube and 2 flavor source bottles (put the filter paper that contains attractant in a bottle, another puts the filter paper that contains contrast agents) respectively.After opening air pump, air-flow before entering flavor source bottle first through an active carbon filter and a distilled water bottle, to purify air and to increase air humidity.The air flow rate of each arm is controlled at 150ml/min by flowmeter.Whole living survey process is indoor the carrying out of 28 ℃.Place a 25W incandescent lamp at 25cm place, the place ahead of olfactometer, with controlled light.
The attractant solution that before experiment, 10 μ l is prepared is added on (long 1.5cm, wide 0.5cm) on the quantitative filter paper sheet, puts into source bottle (long 10.0cm, internal diameter 1.0cm) simply; Separately 10 μ l control solvent are added on the quantitative filter paper sheet, put into another flavor source bottle.Connect experimental instrument, connect air pump.Adopt little dactylethrae that rice lice tassel chalcid fly queen bee is introduced in the straight tube of Y-shaped smell sensing apparatus by head.Observe the behavior reaction of putting down in writing rice lice tassel chalcid fly in 10 minutes, put down in writing it to the selectivity of two arm odor sources.Selection criteria is: when it gets over the 7cm place of a certain side arm and continues 1min when above, remember that this honeybee makes a choice to the odor source of this arm.If chalcid fly does not make one's options after introducing 10min yet, is designated as reactionless.Be the phase mutual interference between the elimination natural enemy and the impact of some other factor, every giving birth to surveyed 2 honeybees just with 99% alcohol wash olfactometer, and dries in 100 ℃ of baking ovens, changes simultaneously olfactometer two arm directions when next secondary survey.Measure altogether 40 of rice lice tassel chalcid flies.To measured result, analyze attractant to the attracting action of rice lice tassel chalcid fly with statistical method.
Data from Fig. 1 can find out, rice planthopper natural enemy rice lice tassel chalcid fly selects the quantity of attractant obviously more than solvent control, and namely attractant has obvious attracting action to rice lice tassel chalcid fly.Utilize the rice planthopper natural enemy attractant that embodiment 7-11 makes to carry out test same as described above, also can obtain similar experiment effect.
Test example 2
The rice planthopper natural enemy rice lice tassel chalcid fly of adopting embodiment 13 to make lures core.Get paddy field, be divided into 6 equal residential quarters, each residential quarter area is 9 ㎡ (the wide guard row of 1m that are of 3m * 3m) and outward enclose.In 6 residential quarters, 3 are made as control group, and 3 are made as processed group.In each residential quarter the location 4 clumps of paddy rice, between each clump paddy rice and and cell edge between all at a distance of 1m.With luring core to hang on the rice clump stem stalk of location, apart from ground 20cm, carry out mark.After 7 days, each site of clip hangs and lures the rice clump of core, records the sum of brown planthopper ovum on every clump of paddy rice and the ovum number of quilt parasitism and prey.Computing and control plot brown planthopper ovum by parasitic rate.
Can find out from the data of Fig. 2, making lure core can significantly improve field rice fulgorid ovum by parasitic rate, namely attractant has obvious attracting action to rice lice tassel chalcid fly.The rice lice tassel chalcid fly attractant making that makes with embodiment 7-11 lures core, and carries out test same as described above, also can reach similar technique effect.
Claims (1)
1. rice lice tassel chalcid fly attractant, it is characterized in that, the component that comprises the following weight proportioning: gaultherolin: suitable-3-hexenoic aldehyde: linalool: suitable acetic acid-3-hexene ester: atoleine: BHT: ESCALOL 567=0.01:0.01:0.01:0.01:0.2:0.01:0.01.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105532762B (en) * | 2015-12-24 | 2018-01-16 | 浙江大学 | A kind of Anagrus nilaparvatae attractant and its application based on plants essential oil |
| CN111226934B (en) * | 2020-03-20 | 2021-08-03 | 上海市农业科学院 | Methyl salicylate parasitic wasp attractant, preparation method and application |
| CN111802389B (en) * | 2020-08-07 | 2021-12-28 | 中国农业科学院茶叶研究所 | Exogenous induction composition, exogenous inducer and method for improving carotenoid in tea |
| CN111903618B (en) * | 2020-08-11 | 2023-04-25 | 福建省农业科学院水稻研究所 | Artificial cultivation method of rice lice thysanoptera (Anagrus nilaparvatae) |
| CN113875753B (en) * | 2021-10-18 | 2022-07-22 | 杭州茶菊科技有限公司 | Attractant for three-rod-tassel apis cerana and tiny schizochytrix delavayi and application thereof |
| CN115769818B (en) * | 2023-01-30 | 2023-05-12 | 华南农业大学 | Application of (cis) -3-hexenol acetate in inducing spodoptera frugiperda to spawn and serve as spodoptera frugiperda attractant |
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