CN116548461A - Application method for preventing tea leafhoppers of tea tree - Google Patents

Application method for preventing tea leafhoppers of tea tree Download PDF

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Publication number
CN116548461A
CN116548461A CN202211231693.6A CN202211231693A CN116548461A CN 116548461 A CN116548461 A CN 116548461A CN 202211231693 A CN202211231693 A CN 202211231693A CN 116548461 A CN116548461 A CN 116548461A
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tea
indoxacarb
tolfenpyrad
leafhoppers
suspending agent
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张通
张少武
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Shaanxi Meibang Pharmaceutical Group Co ltd
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Shaanxi Meibang Pharmaceutical Group Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention relates to the technical field of pesticides, and discloses an application method for preventing tea leafhoppers of tea trees. The application method is to prepare the pesticide composition into a suspending agent according to a certain proportion, and spray the suspending agent according to a certain dosage, so as to prevent and treat tea leafhoppers of tea trees. The pesticide composition consists of indoxacarb, tolfenpyrad and an auxiliary agent; wherein the ratio of indoxacarb to tolfenpyrad is 1:1-1:3. The application method for controlling tea leafhoppers can effectively control pests, improves the effect of controlling tea leafhoppers, has obvious synergism on resistant pests, reduces the pesticide consumption, reduces the pesticide cost of farmers, and is beneficial to the safety and environmental protection of environment and food.

Description

Application method for preventing tea leafhoppers of tea tree
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to an application method for preventing tea leafhoppers of tea trees.
Background
Indoxacarb is a sodium ion channel inhibitor serving as an action mechanism, and is mainly used for blocking sodium ion channels in nerve cells of pests, so that target pests are paralyzed and finally die. The pesticide enters the insect body through contact killing and ingestion, and the behavior of pests is changed rapidly, so that the pests terminate ingestion rapidly, and the target crops are protected better.
The tolfenpyrad is a novel pyrazole heterocyclic insecticidal and acaricidal agent, has a contact-killing effect, has a long lasting period, and can inhibit pests from feeding.
Tea leafhoppers, tea leafhoppers (Empoasca pirisuga Matumura), hemiptera, cicadellidae, commonly known as floating dust seeds and She Tiaochong, occur generally in tea provinces and autonomous areas throughout the country.
The leafhoppers are classified into two kinds of tea leafhoppers and pseudoeye leafhoppers. The tea leafhopper is mainly damaged, and is one of main tea pests. The insects occur for about 8-12 generations in one year, and alternate in generation. The tea is seriously damaged in summer and autumn, the tea tree bud She Quansu, the hardening, the leaf tip and the leaf margin are scorched and are slow in growth, and the tea yield and the quality are greatly affected.
The generation of leafhoppers occurs for 9-11 generations a year, and most of the leafhoppers overwintere tea trees or winter crops (beans), weeds and other plants, and the leafhoppers can overwinter in various insect states in south China. In the next early spring, adults begin to eat and supplement nutrition, and the tea trees begin to spawn and reproduce after sprouting. The bud tips of the tea trees stop growing in the late autumn and early winter, the adults also stop spawning, and the winter is entered. Adults have the habit of successive spawning and batch spawning, and especially overwintering adults can have spawning periods as long as 1 month. Because the generation overlap is serious, the reproductive capacity is strong, the ecological adaptability is strong, the drug resistance is strong, and chemical pesticides are used for preventing and treating for a long time, pests quickly generate extremely high level resistance to various pesticides, and how to provide a product with low toxicity, good prevention effect and obvious effect on resistant pests is a problem to be solved by the technical personnel in the field.
The applicant finds that the two medicaments with completely different action mechanisms are mixed through continuous innovative researches, so that the aim of effectively preventing and controlling pests can be achieved through combined action of the medicaments on the pests from multiple sides, and meanwhile, the dosage of the medicaments in fields is reduced, especially for resistant pests, and the medicaments have quick acting and lasting effects. On the other hand, the residue is small, the safety interval period is short, the pollution of the pesticide to the environment is reduced, and the risk of the pest to the pesticide resistance is also reduced. Therefore, the blending of indoxacarb and tolfenpyrad is worth popularizing and applying in agricultural production.
Disclosure of Invention
The invention aims to provide an application method for preventing and controlling tea leafhoppers, which can effectively prevent and control tea leafhoppers, has quick acting and lasting effects, has obvious effect on resistant pests, and has the advantages of environmental protection and cost reduction.
The technical scheme of the invention is as follows:
an application method for preventing tea leafhoppers of tea trees is characterized by comprising the following steps: the application method is to prepare the pesticide composition into a suspending agent according to a certain proportion, and spray the suspending agent according to a certain dosage, so as to prevent and treat tea leafhoppers of tea trees.
Further, the pesticide composition consists of indoxacarb, tolfenpyrad and an auxiliary agent, wherein the ratio of the indoxacarb to tolfenpyrad is 1:1-1:3, and the preferable ratio is 1:2.5 or 1:2.
Further, the content of the effective active ingredient of the pesticide composition is 10% -50%, preferably 35% or 30%.
Further, the auxiliary agent is one or more selected from dispersing agents, wetting agents, antifreezing agents, preservatives, defoamers, PH regulators, thickening agents and water.
Further, the dosage of the effective components of the pesticide composition is 45-105 g/hectare.
Further, the pesticide composition is applied to control tea leafhoppers in a water consumption amount of 450-750 liters/hectare, preferably 675 liters/hectare.
The suspending agent composition comprises the following components in percentage by weight when being prepared into a suspending agent: 2.5 to 25 percent of indoxacarb, 5 to 37.5 percent of tolfenpyrad, 1 to 10 percent of dispersing agent, 1 to 10 percent of wetting agent, 0.1 to 2 percent of defoaming agent, 0.2 to 3 percent of thickening agent, 0 to 10 percent of antifreezing agent, 0 to 2 percent of preservative, 0 to 2 percent of PH regulator and the balance of water.
Compared with the prior art, the composition provided by the invention has the following beneficial effects: (1) Compared with a single dose, the composition has obvious synergism on tea leafhoppers of tea trees, and obviously improves the control effect; (2) The pesticide is low in toxicity and high in efficiency, the pesticide dosage is reduced, the residual quantity of the pesticide on crops is reduced, and the safety interval period is short; (3) has quick and long-acting effects; (4) delay the drug resistance of pests.
Detailed Description
The present invention will be further illustrated with reference to examples, but the present invention is not limited thereto.
Application example one
EXAMPLE 1 35% indoxacarb tolfenpyrad suspension
The 35% indoxacarb-tolfenpyrad suspending agent is prepared from 95% indoxacarb raw material by 10%, 98% tolfenpyrad raw material by 25%, alkyl naphthalene sulfonate by 2%, fatty alcohol polyoxyethylene ether by 1%, ethylene glycol by 5%, xanthan gum by 0.2%, magnesium aluminum silicate by 1%, organic silicone by 0.3% and water by the balance.
EXAMPLE 2 35% indoxacarb tolfenpyrad suspension
The 35% indoxacarb-tolfenpyrad suspending agent is prepared from 95% indoxacarb technical material by 10%, 98% tolfenpyrad technical material by 25%, alkyl naphthalene sulfonate formaldehyde polymer 1.5%, fatty amine polyoxyethylene ether 2%, propylene glycol 4%, polyvinyl alcohol 0.15%, magnesium aluminum silicate 1%, fatty alcohol 0.2% and the balance of water.
EXAMPLE 3 30% indoxacarb tolfenpyrad suspension
The 30% indoxacarb-tolfenpyrad suspending agent is prepared from 95% indoxacarb technical raw material, 98% tolfenpyrad technical raw material, 20% lignin sulfonate, 3% alkyl glycoside, 5% glycerol, 0.25% xanthan gum, 1% magnesium aluminum silicate, 0.2% sodium benzoate, 0.15% organic silicone and the balance of water.
EXAMPLE 4 15% indoxacarb tolfenpyrad suspension
The suspending agent of the indoxacarb and the tolfenpyrad is prepared by 95 percent of indoxacarb original drug, 98 percent of tolfenpyrad original drug, 10 percent of polycarboxylate, 2.5 percent of polyarylphenol polyoxyethylene ether, 3 percent of glycerol, 4 percent of polyvinyl alcohol, 0.3 percent of sodium benzoate, 0.2 percent of organic silicone and the balance of water.
EXAMPLE 5 42% indoxacarb tolfenpyrad suspension
The suspending agent of 42% indoxacarb and tolfenpyrad is prepared from 95% of indoxacarb crude drug by 12%, 98% of tolfenpyrad crude drug by 30%, EO-PO block copolymer by 1%, alkyl naphthalene sulfonate by 1%, trisiloxane polyoxyethylene ether by 3%, propylene glycol by 5%, xanthan gum by 0.25%, magnesium aluminum silicate by 1%, sodium benzoate by 0.2%, organic silicone by 0.2% and water by the balance.
Implementation example II:
indoxacarb, tolfenpyrad and indoor combined toxicity test of tea leafhopper and mixed tea
1 purpose of test
The toxicity of indoxacarb and tolfenpyrad and different compounding combinations thereof to the tea lesser leafhoppers are measured indoors, the synergism is evaluated, the suitability of the indoxacarb and tolfenpyrad is clear, and scientific basis is provided for research and development of indoxacarb and tolfenpyrad mixtures.
2. Experimental conditions
2.1 Test target
Tea leafhoppers (Empoasca pirisuga Matumura) were harvested from the field and 3-year nymphs of 2 generations were bred with fresh shoots of tea tree in the laboratory.
2. Culture conditions
The culture conditions of the tested target and the tested target are that the temperature is 25+/-1 ℃, the relative humidity is 60% -80%, and the illumination period is L:D= (16:8) h.
3. Test design
3.1 Test agent
Indoxacarb (indoxacarb) 95% active principle and tolfenpyrad (tolfenpyrad) 98% active principle.
3.2 pharmaceutical formulation
Weighing 0.0210g of indoxacarb 95% crude drug, dissolving with 2mL of DMF, adding 0.4mL of Tween80 emulsifier, stirring uniformly, adding clear water to 200mL to prepare 100mg/L mother liquor, adding 60mL of mother liquor into 60mL of water containing 0.1% Tween80 emulsifier to prepare 40mg/L test solution, and diluting to 20, 10, 5, 2.5 and 1.25mg/L according to a proportion of 2 times, wherein 6 concentrations are used for testing.
0.0204g of tolfenpyrad 98% crude drug is weighed, dissolved by 2mL of DMF, added with 0.4mL of Tween80 emulsifier, stirred uniformly, added with clear water to 200mL to prepare 100mg/L mother liquor, taken 80mL of mother liquor to be added with 20mL of water containing 0.1% of Tween80 emulsifier to prepare 80mg/L test solution, and diluted into 40, 20, 10, 5 and 2.5mg/L according to a proportion of 2 times, and 6 concentrations are tested.
According to the proportion of indoxacarb to tolfenpyrad of 2:1, 1:1, 1:2, 1:2.5 and 1:3, respectively weighing 26.67, 20, 13.33, 11.43 and 10mL of 100mg/L indoxacarb mother liquor, respectively weighing 13.33, 20, 26.67, 28.57 and 30mL of 100mg/L tolfenpyrad mother liquor, mixing the two, adding clear water to 100mL, preparing 40mg/L test solution, respectively diluting the test solution into 6 concentrations according to the proportion of 2 times by using water containing 0.1% Tween80 emulsifier, and performing experiments.
98mL of water containing 0.1% Tween80 emulsifier was added to a beaker containing 2mL of DMF as a blank.
4 test method
The dipping method is adopted by referring to the method in the pesticide indoor biological assay test standard NY/T1154.11-2008. Selecting 3 fresh tea tree shoots, soaking in the test liquid for 30 seconds, taking out, naturally airing, wrapping the bases of the shoots with wet absorbent cotton for moisturizing, sealing the absorbent cotton with preservative film, and placing in a glass test tube (phi 200 multiplied by 30 mm) for 4 times per concentration. Then, a writing brush is used for inoculating 3-year nymphs of tea lesser leafhoppers which are subjected to mild anesthesia treatment by diethyl ether, 15+/-2 heads of each tube are connected, and the mouth of each tube is sealed by white gauze. After the treatment, the glass is placed in an observation chamber.
5. Data investigation and statistical analysis
5.1 Investigation time and method
After 72 hours, the death condition of the test insects is checked and recorded, and the death judgment standard of the test insects is that the test insects are gently contacted by forceps and have no response, so that the test insects are dead.
5.2 method for evaluating synergy
And (3) solving the co-toxicity coefficient (CTC) of each compounding combination by adopting a Sun & Johnson (1960) co-toxicity coefficient method according to the indoor biological assay criterion NY/T1154.7-2006 of the pesticide. Generally, CTC.ltoreq.80 is antagonism, 80< CTC <120 is additive, CTC.ltoreq.120 is synergistic. The co-toxicity coefficient calculation formula is as follows:
drug combination Theory Toxicity Index (TTI) =toxicity index of agent a×percent (%) of agent a in the combination+toxicity index of agent b×percent (%)
6 experimental results
The toxicity measurement results of indoxacarb and tolfenpyrad mixed in the proportions of 2:1, 1:1, 1:2, 1:2.5 and 1:3 on tea leafhoppers are shown in Table 1. From the table, the mixed proportion of indoxacarb and tolfenpyrad is 2:1, and the LC of the indoxacarb and tolfenpyrad has synergistic effect 50 The values are 6.47, 6.20, 5.79, 5.67 and 6.41mg/L, respectively, and the co-toxicity coefficients are 114.16, 125.57, 142.13, 147.55 and 132.16, respectively, wherein the synergy at 1:2.5 and 1:2 is most pronounced.
TABLE 1 toxicity measurement results of mixed indoxacarb and tolfenpyrad on tea leafhoppers
The indoor test result shows that indoxacarb and tolfenpyrad are mixed according to the proportion of 1:1, 1:2, 1:2.5 and 1:3 to show synergistic effect on tea leafhoppers, wherein the synergistic effect is most obvious according to the proportion of 1:2.5 and 1:2. Therefore, the optimal proportion of indoxacarb and tolfenpyrad can be seen as 1:2.5 or 1:2 only from the synergistic toxicity comparison.
Application example three: example 1 purpose of field efficacy test 1 for controlling tea leafhopper
The 35% indoxacarb-tolfenpyrad suspending agent developed by the applicant is verified to have the control effect on tea leafhoppers of tea trees and the safety on tea trees, the field use dosage and the application technology are defined, and a scientific basis is provided for pesticide registration.
2 basis of test
Pesticide registration field efficacy test quality management Specification (NY/T2885-2016) of agricultural industry Standard of the people's republic of China; pesticide field efficacy test criterion (II) (GB/T17980.56-2004).
3 experiment site
The test was set up in Yu Chuan town in Wu cave city of Hubei province.
4 selection of test subjects, crops and varieties
Test object: tea leafhoppers (Empoasca pirisuga);
test crop: the tea tree is Fuding big white tea, and the tree age is 5 years.
5 test design and arrangement
5.1 dosage and numbering of the medicament
TABLE 2 test design of reagents for test
Treatment of Test object Dosage of the preparation is mL/mu Dosage of active ingredient g/hm 2
A 35% indoxacarb tolfenpyrad suspending agent 10 52.5
B 35% indoxacarb tolfenpyrad suspending agent 15 78.75
C 35% indoxacarb tolfenpyrad suspending agent 20 105
D 150g/L indoxacarb emulsifiable concentrate 22 49.5
E 15% tolfenpyrad suspending agent 50 112.5
CK Clear water control /
5.2 times and times of application
Once in 2021, 6 and 24 days.
5.3 usage Capacity
The chemical solution is sprayed to each mu of the crops in 45 liters, and the liquid amount sprayed per hectare is 675 liters.
5.4 investigation time and times
The number of insect population was investigated before treatment, and 1, 3 and 7 days after treatment were investigated respectively.
When the morning dew was not dry, the number of nymphs on 30 shoot tips (3, 4 leaves of one bud) per cell was investigated randomly.
5.5 method for calculating efficacy
6 experimental results
TABLE 3 field control of tea leafhoppers on tea plant
TABLE 4 example 1 results of field efficacy test for controlling tea leafhoppers
The 35% indoxacarb-tolfenpyrad suspending agent for the test object in the embodiment 1 has good quick acting performance on tea leafhoppers, and the prevention effect on tea leafhoppers is 88.47% -95.06% after 1 day of the drug treatment, wherein the reduction rate of insect mouths of the tea leafhoppers is 88.08% -94.93% under the treatment of 10 mL/mu, 15 mL/mu and 20 mL/mu of the test object; the control substance has the control effects of being treated with 22 mL/mu of indoxacarb emulsifiable concentrate and 50 mL/mu of 15% tolfenpyrad suspending agent on tea leafhoppers of the tea trees of 87.46% and 85.31% respectively, and the control effects are lower than those of the test substance treated with 15 mL/mu on the tea leafhoppers of the tea trees; the control effect of each dose of the tested object on tea leafhoppers is improved by 94.51-98.73% compared with that of the tested object on 1 day after the test object is treated.
Example 1 the tested object 35% indoxacarb and tolfenpyrad suspending agent has good control effect on tea leafhoppers of tea trees, the reduction rate of the insect mouths of the leafhoppers reaches more than 98.3% under the treatment of each dose of the tested object, the control effect is between 98.36% and 100%, the control object 150g/L indoxacarb emulsifiable concentrate is treated by 22 mL/mu, the control effect of the 15% tolfenpyrad suspending agent is respectively 95.87% and 94.67% on the tea leafhoppers of tea trees under the treatment of 15 mL/mu of the tested object.
The DPS software is used for carrying out difference significance analysis on the prevention effect of 7 days after the medicine by applying the Duncan new compound polar difference method, and the result shows that the difference between the prevention effect of the 35% indoxacarb-tolfenpyrad suspending agent of the tested object between 10 mL/mu treatment and 20 mL/mu treatment on tea leafhoppers is not obvious. The treatment of the tested object with 35% indoxacarb and tolfenpyrad suspending agent with 15 mL/mu is obviously different from the treatment of the control object with 150g/L indoxacarb emulsifiable concentrate with 22 mL/mu, and the treatment of the tested object with 15% tolfenpyrad suspending agent with 50 mL/mu, so that the prevention effect of the tested object is better. It can be seen that the 35% indoxacarb-tolfenpyrad suspending agent as a test object is applied to the low-age larva stage of tea leafhoppers, and has good control effect on the tea leafhoppers under the condition of the reagent supply amount.
In the whole test process, no phytotoxicity is found to be generated on leaves and plants of the tested crops by applying the test drugs, and no adverse effect is found to be generated on the surrounding environment.
Application example four: example 2 field efficacy test for controlling tea leafhopper
The test purpose, the test basis, the water consumption, the calculation method and the formula are the same as those of the application embodiment III.
1 experiment site
Wu Xueshi Mei Chuanzhen by Hubei province.
2 selection of test subjects, crops and varieties
Test object: tea leafhopper (Empoasca pirisuga), tea tree is green tea, and the tree is 5 years old.
3 times and times of application
The tea leafhopper is in the nymph generating period for 1 time after 2022, 6 and 17 days.
4 test design and arrangement
TABLE 5 test design sheet for test object
Treatment of Test object Dosage of the preparation Dosage of active ingredient
A 35% indoxacarb tolfenpyrad suspending agent 10 mL/mu 52.5g/hm 2
B 35% indoxacarb tolfenpyrad suspending agent 15 mL/mu 78.75g/hm 2
C 35% indoxacarb tolfenpyrad suspending agent 20 mL/mu 105g/hm 2
D 150g/L indoxacarb emulsifiable concentrate 22 mL/mu 49.5g/hm2
E 15% tolfenpyrad suspending agent 50 mL/mu 112.5g/hm2
F 20% bifenthrin aqueous emulsion 15 mL/mu 45g/hm2
CK Clear water control / /
5 investigation time, times and method
When the morning dew was not dry, the number of nymphs on 30 shoot tips (3, 4 leaves of one bud) per cell was investigated randomly.
The insect population cardinality was investigated before treatment, and the control effect was investigated 1, 3 and 7 days after treatment. A total of 4 surveys.
6 experimental results
TABLE 6 example 2 field control of tea leafhoppers-insect-mouth reduction Rate
TABLE 7 example 2 field controlling Effect on tea leafhoppers
Note that lowercase letters indicate differences between treatments at the 0.05 level and uppercase letters indicate differences between treatments at the 0.01 level.
The 35% indoxacarb-tolfenpyrad suspending agent for the test object in the embodiment 2 has good quick acting performance on tea leafhoppers, and the insect mouth decline rate of the tea leafhoppers under the treatment of 10 mL/mu, 15 mL/mu and 20 mL/mu of the test object is between 83.80 and 87.22 percent and the prevention effect on the tea leafhoppers is between 83.76 and 87.17 percent 1 day after the test object is treated; the control substance has the control effects of 82.66 percent, 81.71 percent and 80.50 percent on the prevention effect of the control substance of 150 g/liter indoxacarb emulsifiable concentrate of 22 mL/mu, 15 percent of tolfenpyrad suspending agent of 50 mL/mu and 20 percent of bifenthrin aqueous emulsion of 15 mL/mu; 3 days after the medicine, the control effect of each dose of the tested object on the tea plant leafhoppers is obviously improved compared with 1 day after the medicine, and the control effect is between 93.32 and 96.70 percent; the control substance has the control effects of 92.13 percent, 90.70 percent and 89.80 percent on the treatment of the indoxacarb emulsifiable concentrate with the concentration of 150 g/liter, the treatment of the tolfenpyrad with the concentration of 15 percent, the treatment of the tolfenpyrad with the concentration of 50 mL/mu, and the treatment of the bifenthrin with the concentration of 20 percent and the aqueous emulsion with the concentration of 15 mL/mu.
The 35% indoxacarb and tolfenpyrad suspending agent of the tested object has excellent prevention effect on tea leafhoppers of tea trees, the reduction rate of the insect mouths of the tea leafhoppers is between 95.19 and 98.86% and the prevention effect is between 95.83 and 98.99% under the treatment of each dose of the tested object, the control object is treated by 22 mL/mu of indoxacarb emulsifiable concentrate, the 15% tolfenpyrad suspending agent is treated by 50 mL/mu, and the prevention effect of the 20% bifenthrin aqueous emulsion is 93.94%, 93.96% and 92.90% respectively.
Using DPS software, applying Duncan new complex polar error method to analyze the difference significance of the prevention effect result 7 days after the medicine, the result shows that: although the prevention effect of the tested object 35% indoxacarb-tolfenpyrad suspending agent is obviously different from that of the tested object 20 mL/mu, the prevention effect is more than 90%; the prevention effect difference between the 15 mL/mu treatment of the tested object and the 150 g/mu treatment of the control object, the 22 mL/mu treatment of the indoxacarb emulsifiable concentrate, the 50 mL/mu treatment of the 15% tolfenpyrad suspending agent and the 15 mL/mu treatment of the 20% bifenthrin aqueous emulsion is obvious, and the effect of the tested object is better.
In a word, the 35% indoxacarb-tolfenpyrad suspending agent has a good control effect on tea leafhoppers of tea trees, and experiments show that the 35% indoxacarb-tolfenpyrad suspending agent has no obvious influence on leaf color and plant growth state of tea trees within a test dosage range, and is safe and harmless to the tea trees.
Application example five: example 3 test purpose, test basis, water consumption, calculation method and formula of field efficacy test for controlling tea leafhopper of tea are the same as those of application example three.
1 experiment site
Wu Xueshi Mei Chuanzhen by Hubei province.
2 selection of test subjects, crops and varieties
Test object: tea leafhopper (Empoasca pirisuga), tea tree is green tea, and the tree is 5 years old.
3 times and times of application
The tea leafhopper is in the nymph occurrence stage for 1 time after 2022, 6 and 17 days.
4 test design and arrangement
TABLE 8 test design sheet for test object
Treatment of Test object Dosage of the preparation Dosage of active ingredient
A 30% indoxacarb tolfenpyrad suspending agent 10 mL/mu 45g/hm2
B 30% indoxacarb tolfenpyrad suspending agent 15 mL/mu 67.5g/hm2
C 30% indoxacarb tolfenpyrad suspending agent 20 mL/mu 90g/hm2
D 150g/L indoxacarb emulsifiable concentrate 22 mL/mu 49.5g/hm2
E 15% tolfenpyrad suspending agent 50 mL/mu 112.5g/hm2
F 20% bifenthrin aqueous emulsion 15 mL/mu 45g/hm2
CK Clear water control / /
5 investigation time, times and method
When the morning dew was not dry, the number of nymphs on 30 shoot tips (3, 4 leaves of one bud) per cell was investigated randomly.
The insect population cardinality was investigated before treatment, and the control effect was investigated 1, 3 and 7 days after treatment. A total of 4 surveys.
6 experimental results
Table 9 example 3 field control of tea leafhoppers-rate of reduction of insect population
TABLE 10 example 3 field controlling Effect on tea leafhoppers
The 30% indoxacarb-tolfenpyrad suspending agent for the test object in the embodiment 3 has good quick acting performance on tea leafhoppers, and the insect mouth decline rate of the tea leafhoppers under the treatment of 10 mL/mu, 15 mL/mu and 20 mL/mu of the test object is 80.95-87.02% and the prevention effect on the tea leafhoppers is 81.50-87.40% 1 day after the test object is treated; the control substance has the control effects of 80.40 percent, 78.41 percent and 80.36 percent on the treatment of 22 mL/mu of indoxacarb emulsifiable concentrate, 50 mL/mu of 15 percent of tolfenpyrad suspending agent and 15 mL/mu of 20 percent of bifenthrin aqueous emulsion; 3 days after the medicine, the control effect of each dose of the tested object on the tea plant leafhoppers is obviously improved compared with 1 day after the medicine, and the control effect is 91.51-95.76%; the control substance has the control effects of 89.77 percent, 88.26 percent and 90.37 percent on the treatment of the indoxacarb emulsifiable concentrate with the concentration of 150 g/liter, the treatment of the tolfenpyrad with the concentration of 15 percent, the treatment of the tolfenpyrad suspending agent with the concentration of 50mL/667m2, and the treatment of the bifenthrin aqueous emulsion with the concentration of 20 percent, wherein the control substance has the control effects of 89.77 percent, 88.26 percent and 90.37 percent on the tea leafhoppers with the concentration of 15 mL/mu.
The tested object 30% indoxacarb and tolfenpyrad suspending agent has excellent prevention effect on tea leafhoppers of tea trees, the reduction rate of the insect mouths of the tea leafhoppers is 93.24-97.46% under the treatment of each dose of the tested object, the prevention effect is 94.16-97.86%, the prevention effect of the control object is 150 g/liter of indoxacarb emulsifiable concentrate 22 mL/mu treatment, 15% tolfenpyrad suspending agent 50 mL/mu treatment and 20% bifenthrin aqueous emulsion 15 mL/mu treatment on the tea leafhoppers is 92.49%, 92.41% and 92.83% respectively.
Using DPS software, applying Duncan new complex polar error method to analyze the difference significance of the prevention effect result 7 days after the medicine, the result shows that: the prevention effect of the tested object is obviously different between the treatment of 30% indoxacarb and tolfenpyrad suspending agent of 10 mL/mu and the treatment of 20 mL/mu, and the prevention effect of the tested object is obviously different between the treatment of 15 mL/mu and the treatment of 150 g/liter indoxacarb emulsifiable concentrate of control object of 22 mL/mu, the treatment of 15% tolfenpyrad suspending agent of 50 mL/mu and the treatment of 20% bifenthrin aqueous emulsion of 15 mL/mu. The effect of the tested object is better.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and these should be considered to be within the scope of the invention.

Claims (7)

1. An application method for preventing tea leafhoppers of tea trees is characterized by comprising the following steps: the application method is to prepare the pesticide composition into a suspending agent according to a certain proportion, and spray the suspending agent according to a certain dosage, so as to prevent and treat tea leafhoppers of tea trees.
2. The method of administration of claim 1, wherein: the pesticide composition consists of indoxacarb and tolfenpyrad, and an auxiliary agent, wherein the ratio of the indoxacarb to tolfenpyrad is 1:1-1:3.
3. The method of administration of claim 2, wherein: the ratio of indoxacarb to tolfenpyrad is 1:2.5 or 1:2.
4. A method of administration according to any one of claims 1 to 3, wherein: the content of the effective active ingredients of the pesticide composition is 10% -50%.
5. The method of administration of claim 4, wherein: the content of the effective active ingredients of the pesticide composition is 35% or 30%.
6. The method of administration of claim 2, wherein: the auxiliary agent is one or more selected from dispersing agent, wetting agent, antifreezing agent, preservative, defoamer, PH regulator, thickener and water.
7. The method of administration of claim 1, wherein: the dosage of the effective components of the pesticide composition is 45-105 g/hectare.
CN202211231693.6A 2022-10-08 2022-10-08 Application method for preventing tea leafhoppers of tea tree Pending CN116548461A (en)

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