CN105123803A - Botanical fungicide containing thymol and kasugamycin - Google Patents
Botanical fungicide containing thymol and kasugamycin Download PDFInfo
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Abstract
The invention relates to a botanical fungicide containing thymol and kasugamycin, in particular to a formula and preparing method of a botanical fungicide containing kasugamycin, thymol, radix isatidis active ingredients and betel nut active ingredients. The botanical fungicide is prepared from, by weight, 0.1-5% of kasugamycin, 1-50% of thymol, 1-25% of radix isatidis active ingredients, 1-30% of betel nut active ingredients and the balance auxiliaries. According to the botanical fungicide containing thymol and kasugamycin, synergism is realized through blending. The botanical fungicide is wide in application range, low in cost and good in effect, can be used for controlling diseases, including downy mildew and pestilence, occurring on multiple crops such as grapes, cucumbers, tomatoes and potatoes, caused by fungi such as phycomycetes, can also be used for treating bacterial diseases such as cucumber bacterial angular leaf spots, has a good control effect, and can well delay the drug resistance of pathogenic bacteria.
Description
Technical field
The present invention relates to a kind of lawn plant source bactericide, the formula of the botanical fungicide particularly containing kasugarnycin, Thymol, Radix Isatidis active component, betel nut active component, and preparation method.
Background technology
Kasugarnycin (Kasugamycin), also known as kasugarnycin, kasumin, is a kind of antibiotics low toxicity bactericide.Metabolism of amino acid can be disturbed to affect the synthesis of protein, suppress hyphal elongation and cause cell granulations, but on spore germination without impact.Have stronger systemic activity, its result for the treatment of is more remarkable, is the various bacteria of crop such as control vegetables, melon and fruit etc. and the favourable agents of fungal disease, and the melon leaf look after dispenser can be made dark green and delay harvest time.After kasugarnycin is applied to the crops such as paddy rice, through Uptake and translocation in plant corpus, suppress synthesis and even the mycelial growth of rice blast protein significantly, peptide bond is elongated, the activity of peptide for inhibiting transferase.
Thymol because of initial from thyme extraction and isolation out gain the name, it is a kind of semi-transparent clear crystallization, is very easily dissolved in ethanol, ether, acetic acid, chloroform and benzene, has certain fragrance and has very strong bactericidal action.On June 19th, 2013, EU Committee has ratified Thymol to put on market as plant protection products, and the safety of apparent Thymol is very high, toxicity is also lower.Thymol also has very wide application such as owing to having antimycotic and parasite function in other side at present, can be used to process wound, makes preservative, medical bactericide, store dissec etc.; Because it has phenols smell, be also usually used in pest repellant and spices etc.In addition, Thymol also can be used as antioxidant, the qualification special reagent of titanium nitride, the contrast benchmark of thymol blue.Thymol is a kind of active skull cap components, and safety is high, is easy to obtain, and stable chemical nature, toxicity is low, easily degrades in the environment, the demand for development of these characteristic conforms biopesticides.
Containing many kinds of substance in Areca, as compositions such as fat, betel oil, alkaloid, catechin, choline.Betel nut infusion has inhibitory action in various degree to dermatophytes such as trichophytons in vitro.Decoction and water logging agent infected by influenza have certain inhibitory action.Be one of China's rare medicinal herbs, can stagnation of QI due to dyspepsia be cured, abdominal distention, oedema, beriberi, the illnesss such as malaria.Modern pharmacology experiment also proves, betel nut have antiviral, control gout and the effect such as antimycotic.
Radix Isatidis has broad-spectrum antibacterial action, be anti-inflammation, pain relieving, the Chinese traditional herbs of bringing down a fever.Infusion has inhibitory action to hay bacillus, staphylococcus aureus, sarcine, Escherichia coli, typhoid bacillus, paratyphoid A bacillus, dysentery (will Hayes, Fu Shi) bacillus, bacillus enteritidis etc.
Because medicament is used alone for a long time, easily cause the generation of germ and pest resistance to insecticide, the method that solution pesticide resistance is commonly used is undertaken composite by the active ingredient of the different mechanism of action.A kind of bactericide containing kasugarnycin, Thymol, betel nut and Radix Isatidis provided by the invention has synergistic function, and complex role is obvious, greatly reduces the dosage of agricultural chemicals, also can expand fungicidal spectrum, avoid environmental pollution, improves germicidal efficiency.Utilize the bactericidal activity of Thymol, betel nut and Radix Isatidis and can to the effect of bactericide synergy, development of new can actual production can be applicable to the bactericide product of crop disease control, and about the bactericide of kasugarnycin and Thymol, there is not yet relevant report at home and abroad at present.
Summary of the invention
The invention provides and a kind of there is low, the effective botanical fungicide containing kasugarnycin and Thymol of synergistic function, use cost.
The botanical fungicide that the invention provides a kind of kasugarnycin and Thymol comprises wetting powder, suspending agent, water dispersible granules, adds auxiliary agent and excipient, and the production technology all known according to those skilled in the art can be made into required formulation.
A kind of plant resource bactericidal suspension emulsion containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5 ~ 3%,
Thymol 1% ~ 30%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Emulsifier 2% ~ 8%,
Dispersant 0.5% ~ 5%,
Wetting agent 0.1% ~ 5%,
Thickener 0.5% ~ 6%,
Defoamer 0.1% ~ 0.5%,
Surplus is water.
Wherein said water is the one in running water, distilled water or deionized water.The weight percentage of described Thymol is preferably 10 ~ 20%.Described emulsifier is preferably Tripolyglycerol monostearates.Described dispersant is preferably sodium lignin sulfonate.Described wetting agent is preferably lauryl sodium sulfate.Described thickener is preferably xanthans.Described defoamer is preferably mineral oil.The weight percentage of described kasugarnycin is preferably 1% ~ 3%.The weight percentage of described Thymol is preferably 10% ~ 20%.The weight percentage of described betel nut active component is preferably 5% ~ 10%.The weight percentage of described Radix Isatidis active component is preferably 2 ~ 6%.The weight percentage of described solvent is preferably 10% ~ 30%.The weight percentage of described emulsifier is preferably 3% ~ 6%.The weight percentage of described dispersant is preferably 1% ~ 3%.The weight percentage of described wetting agent is preferably 0.5% ~ 2%.The weight percentage of described thickener is preferably 1% ~ 3%.The weight percentage of described defoamer is preferably 0.15% ~ 0.25%.
Kasugarnycin, Thymol, betel nut active component and Radix Isatidis active component are added dispersant, wetting agent, emulsifier, thickener, defoamer by a kind of plant resource bactericidal suspension emulsion preparation method containing kasugarnycin and Thymol provided by the present invention successively, surplus adds water, stirring to dissolving completely, mixing and obtaining the bactericidal suspension emulsion of kasugarnycin and Thymol.
A kind of plant resource sterilized water dispersible granule containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5% ~ 2%,
Thymol 1% ~ 40%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Carrier 40% ~ 85%,
Dispersant 0.2% ~ 5%,
Wetting agent 1% ~ 12%,
Disintegrant 0.3% ~ 13%.
Wherein said carrier is preferably kaolin, white carbon, and described dispersant is preferably sodium lignin sulfonate, and described wetting agent is preferably DBSA, and described disintegrant is preferably dried starch.The weight percentage of described Thymol is preferably 10% ~ 30%.The weight percentage of described carrier is preferably 50% ~ 80%.The weight percentage of described dispersant is preferably 0.5% ~ 2%.The weight percentage of described wetting agent is preferably 2% ~ 6%.The weight percentage of described disintegrant is preferably 1% ~ 5%.
A kind of plant resource sterilized water dispersible granule preparation method containing kasugarnycin and Thymol provided by the present invention be former for Thymol medicine is added successively kasugarnycin, betel nut active component, Radix Isatidis active component, kaolin, sodium lignin sulfonate drop in screw mixer and stir, carry out air-flow crushing again, carry out mixed at high speed granulation after pulverizing, be the plant resource sterilized water dispersible granule of kasugarnycin and Thymol.
A kind of plant resource sterilization wettable powder containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5% ~ 2%,
Thymol 1% ~ 40%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Carrier 10% ~ 60%,
Dispersant 0.2% ~ 5%,
Wetting agent 1% ~ 10%,
Bleeding agent 1% ~ 9%.
Wherein said carrier is preferably kaolin, white carbon, and described dispersant is preferably sodium lignin sulfonate, and described wetting agent is preferably DBSA, and described bleeding agent is preferably fatty alcohol-polyoxyethylene ether.The weight percentage of described Thymol is preferably 20% ~ 45%.Described carrier is preferably the weight percentage of dispersant described in 30% ~ 60%. and is preferably 0.5% ~ 2%.The weight percentage of described wetting agent is preferably 1% ~ 5%.The weight percentage of described bleeding agent is preferably 2% ~ 6%.Its preparation method former for Thymol medicine, kasugarnycin, betel nut active component, Radix Isatidis active component, kaolin, sodium lignin sulfonate and fatty alcohol-polyoxyethylene ether is dropped in screw mixer to stir, then put into cracker and pulverize.Be Thymol sterilization wettable powder.
The invention provides a kind of botanical fungicide containing kasugarnycin and Thymol, in wherein filling a prescription, the extracting method of betel nut active component is for taking betel nut, first add ammoniacal liquor moistening, with ether lixiviate, sucking-off ether extracted liquid obtains filtrate, residue separately uses washed with diethylether 3 times, merges ether solution, volatilizes under normal temperature.With chloroform recovery dry extract, sucking-off, filters, then with chloroform 3 times, combined chloroform extract, namely vacuum drying obtain betel nut active component.
The invention provides a kind of botanical fungicide containing kasugarnycin and Thymol, wherein in formula, the extracting method of Radix Isatidis active component, for getting chromatogram of Radix Isatidis, is ground into meal, boiling secondary. 2 hours first times. second time l hour, collecting decoction, filters, filtrate concentrates, let cool, add ethanol and make alcohol content reach 80%, stir evenly, leave standstill 24 hours, filter, filtrate recycling ethanol is also concentrated into thick paste, vacuum drying and get final product.
Result of the test shows, product of the present invention has the advantages such as synergistic effect is obvious, quick-acting good, the lasting period is long, highly-effective low-residue, to crop, people and animals and beneficial organism safety, substantially pollution-free to environment.Match with current market demand, there are very large market prospects.
A kind of plant resource sterilization containing kasugarnycin and Thymol provided by the present invention, play synergistic effect by composite. applied widely, cost is low, effective, can be used to the disease such as downy mildew, epidemic disease that the fungies such as control Phycomycetes produce in the various crop such as grape, cucumber, tomato, potato, and doublely can control bacterial disease as cucumber bacterial angular leaf spot etc., have good control efficiency, the pesticide resistance delaying pathogen time simultaneously has good effect.
Embodiment
Embodiment one: take betel nut 10kg, first adds the moistening 10min of ammoniacal liquor 8L, and with 120L ether lixiviate 1 hour, separate out ether extracted liquid, residue separately used washed with diethylether 3 times, each 20L, merged ether solution, volatilized under normal temperature.Extract dry extract with chloroform 20L, separate out, filter, then with chloroform 3 times, each 8L, combined chloroform extract, namely vacuum drying obtain betel nut active component.
Embodiment two: get chromatogram of Radix Isatidis 10kg, be ground into meal, boiling secondary.2 hours first times. second time l hour, collecting decoction, filter, filtrate is concentrated into the concentrate that relative density is 1.15-1.20, lets cool, adding 10L ethanol makes alcohol content reach 80%, stir evenly, leave standstill 24 hours, filter, filtrate recycling ethanol is also concentrated into the thick paste that relative density is 1.32-1.35, and namely vacuum drying obtain Radix Isatidis active component.
Embodiment three: a kind of plant resource bactericidal suspension emulsion containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 2%,
Thymol 20%,
Betel nut active component 6%,
Radix Isatidis active component 6%,
Tripolyglycerol monostearates 8%,
Sodium lignin sulfonate 5%,
Lauryl sodium sulfate 2.5%,
Xanthans 3%,
Mineral oil 0.4%,
Surplus is water.
Get kasugarnycin 200g, Thymol 2kg, betel nut active component 600g and Radix Isatidis active component 600g and add sodium lignin sulfonate 500g, lauryl sodium sulfate 250g, Tripolyglycerol monostearates 800g, xanthans 300g, mineral oil 40g successively, add water to 10kg, stirring to dissolving completely, mixing and obtaining the bactericidal suspension emulsion of kasugarnycin and Thymol.
Embodiment four: a kind of plant resource sterilized water dispersible granule containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 1.5%,
Thymol 25%,
Betel nut active component 8%,
Radix Isatidis active component 8%,
Kaolin 53.7%,
Sodium lignin sulfonate 1.2%,
DBSA 2%,
Dried starch 0.6%.
Its preparation method is added successively by former for Thymol medicine 2.5kg in kasugarnycin 150g, betel nut active component 800g, Radix Isatidis active component 800g, kaolin 5.37kg, sodium lignin sulfonate 120g, DBSA 200g, dried starch 60g input screw mixer to stir, carry out air-flow crushing again, carry out mixed at high speed granulation after pulverizing, be the plant resource sterilized water dispersible granule of kasugarnycin and Thymol.
Embodiment five: a kind of plant resource sterilization wettable powder containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 2%,
Thymol 23%,
Betel nut active component 10%,
Radix Isatidis active component 10%,
Kaolin 51.3%,
Sodium lignin sulfonate 1%,
DBSA 1.2%,
Fatty alcohol-polyoxyethylene ether 1.5%,
Its preparation method is by former for Thymol medicine 2.3kg, kasugarnycin 200g, betel nut active component 1kg, Radix Isatidis active component 1kg, kaolin 5.13kg, sodium lignin sulfonate 100g, DBSA 120g and fatty alcohol-polyoxyethylene ether 150g, drops in screw mixer and stir, then puts into cracker and pulverize.Be Thymol sterilization wettable powder.
Embodiment six: do the indoor pharmacodynamic test preventing and treating tomato downy mildew with the above different kasugarnycin of preparation and the botanical fungicide of Thymol, adopt indoor pot tomato seedlings, measure single mixture of kasugarnycin and Thymol to the control efficiency of tomato downy mildew.When tomato grows to 3-4 sheet leaf, the blade selecting growing way evenly to cause adopts manual sprayer to carry out foliar spray process and inoculates pathogen after .24 hour, and 7d " Invest, Then Investigate " result is cultivated in moisturizing, the results are shown in Table 1.
Table 1 embodiment 1-3 prevents and treats tomato downy mildew indoor pharmacodynamic test result table
| Reagent agent | Dosage (μ g/ml) | Disease refers to | Preventive effect (%) |
| Embodiment three products | 200 | 4.84 | 92.01 |
| Embodiment four-product | 200 | 3.73 | 93.84 |
| Embodiment five product | 200 | 5.23 | 91.37 |
| 2% kasugarnycin | 200 | 30.28 | 50.02 |
| 25% Thymol | 200 | 23.21 | 61.69 |
| CK | - | 60.59 | - |
Embodiment seven: do the field trial of preventing and treating tomato downy mildew, place with the above different kasugarnycin of preparation and the botanical fungicide of Thymol: Chengmai, Hainan.6 process are established in test altogether, are respectively: 1000 times of embodiment three-five, and 10% kasugarnycin and 10% Thymol 1000 times and clear water contrast, and repeat 3 times, plot area 10m
3, district's group randomized arrangement, surrounding does not establish protection row, encloses film protection during dispenser.Observed result after 7 days, the results are shown in Table 2.
Table 2 embodiment 1-3 prevents and treats tomato downy mildew field control effectiveness test result table
From table 1,3 kinds of bactericidal agents that the 2 known embodiment of the present invention obtain all have synergistic effect, and the water dispersible granules effect of the present invention wherein in embodiment four is particularly outstanding.
Claims (7)
1. a kind of plant resource bactericidal suspension emulsion containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5% ~ 3%,
Thymol 1% ~ 30%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Emulsifier 2% ~ 8%,
Dispersant 0.5% ~ 5%,
Wetting agent 0.1% ~ 5%,
Thickener 0.5% ~ 6%,
Defoamer 0.1% ~ 0.5%,
Surplus is water;
It is characterized in that water is the one in running water, distilled water or deionized water; Described emulsifier is preferably Tripolyglycerol monostearates; Described dispersant is preferably sodium lignin sulfonate; Described wetting agent is preferably lauryl sodium sulfate; Described thickener is preferably xanthans; Described defoamer is preferably mineral oil; The weight percentage of described kasugarnycin is preferably 1% ~ 3%; The weight percentage of described Thymol is preferably 10% ~ 20%; The weight percentage of described betel nut active component is preferably 5% ~ 10%; The weight percentage of described Radix Isatidis active component is preferably 2 ~ 6%; The weight percentage of described solvent is preferably 10% ~ 30%; The weight percentage of described emulsifier is preferably 3% ~ 6%; The weight percentage of described dispersant is preferably 1% ~ 3%; The weight percentage of described wetting agent is preferably 0.5% ~ 2%; The weight percentage of described thickener is preferably 1% ~ 3%; The weight percentage of described defoamer is preferably 0.15% ~ 0.25%.
2. a kind of plant resource bactericidal suspension emulsion containing kasugarnycin and Thymol according to claim 1, it is characterized in that its preparation method is that kasugarnycin, Thymol, betel nut active component and Radix Isatidis active component are added dispersant, wetting agent, emulsifier, thickener, defoamer successively, surplus adds water, stirring to dissolving completely, mixing and obtaining the bactericidal suspension emulsion of kasugarnycin and Thymol.
3. a kind of plant resource sterilized water dispersible granule containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5% ~ 2%,
Thymol 1% ~ 40%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Carrier 40% ~ 85%,
Dispersant 0.2% ~ 5%,
Wetting agent 1% ~ 12%,
Disintegrant 0.3% ~ 13%;
It is characterized in that described carrier is preferably kaolin, white carbon, described dispersant is preferably sodium lignin sulfonate, and described wetting agent is preferably DBSA, and described disintegrant is preferably dried starch; The weight percentage of described Thymol is preferably 10% ~ 30%; The weight percentage of described carrier is preferably 50% ~ 80%; The weight percentage of described dispersant is preferably 0.5% ~ 2%; The weight percentage of described wetting agent is preferably 2% ~ 6%; The weight percentage of described disintegrant is preferably 1% ~ 5%.
4. a kind of plant resource sterilized water dispersible granule containing kasugarnycin and Thymol according to claim 3, it is characterized in that its preparation method is added successively by former for Thymol medicine in kasugarnycin, betel nut active component, Radix Isatidis active component, kaolin, sodium lignin sulfonate input screw mixer to stir, carry out air-flow crushing again, carry out mixed at high speed granulation after pulverizing, be the plant resource sterilized water dispersible granule of kasugarnycin and Thymol.
5. a kind of plant resource sterilization wettable powder containing kasugarnycin and Thymol provided by the present invention, is made up of the component of following weight percent:
Kasugarnycin 0.5% ~ 2%,
Thymol 1% ~ 40%,
Betel nut active component 2% ~ 19%,
Radix Isatidis active component 1% ~ 20%,
Carrier 10% ~ 60%,
Dispersant 0.2% ~ 5%,
Wetting agent 1% ~ 10%,
Bleeding agent 1% ~ 9%;
It is characterized in that described carrier is preferably kaolin, white carbon; Described dispersant is preferably sodium lignin sulfonate; Described wetting agent is preferably DBSA; Described bleeding agent is preferably fatty alcohol-polyoxyethylene ether; The weight percentage of described Thymol is preferably 20% ~ 45%; Described carrier is preferably 30% ~ 60%; The weight percentage of described dispersant is preferably 0.5% ~ 2%; The weight percentage of described wetting agent is preferably 1% ~ 5%; The weight percentage of described bleeding agent is preferably 2% ~ 6%.
6. a kind of plant resource sterilization wettable powder containing kasugarnycin and Thymol according to claim 5, it is characterized in that its preparation method former for Thymol medicine, kasugarnycin, betel nut active component, Radix Isatidis active component, kaolin, sodium lignin sulfonate and fatty alcohol-polyoxyethylene ether is dropped in screw mixer to stir, then put into cracker and pulverize.Be Thymol sterilization wettable powder.
7. according to the botanical fungicide containing kasugarnycin and Thymol that claim 1,3,5 provides, it is characterized in that can be used to preventing and treating the disease such as downy mildew, epidemic disease that the fungies such as Phycomycetes produce in the various crop such as grape, cucumber, tomato, potato, and doublely can control bacterial disease as cucumber bacterial angular leaf spot etc., have good control efficiency, the pesticide resistance delaying pathogen time simultaneously has good effect.
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| CN116019101B (en) * | 2021-08-30 | 2023-10-13 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Synergistic composition containing thymol for preventing and treating papaya mites |
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