KR101232936B1 - Simultaneous Controlling Agent for Plant Diseases Comprising Strawberry Pests Comprising Botanical Extract and Controlling Method Using The Same - Google Patents

Abstract

The present invention provides a plant disease control agent, including strawberry gray mold and powdery mildew, which contains as an active ingredient rhubarb root or its extract, and according to the present invention simultaneously control plant diseases including strawberry gray mold and powdery mildew It provides an effect that can be done.

Description

Simultaneous Controlling Agent for Plant Diseases Comprising Strawberry Pests Comprising Botanical Extract and Controlling Method Using The Same}

The present invention relates to a simultaneous control of plant diseases including strawberry ash fungus and powdery mildew disease and a method for controlling the same, and more particularly, by spraying on the leaf zone of crops to inhibit the growth of the crop growth period to significantly increase the number of crops In addition, it can effectively suppress gray mold and powdery mildew that occur in strawberries, and produce high value-added organic products by controlling environmentally friendly fungal diseases that cause serious losses to strawberries that are intensively grown in the facility. It relates to a plant disease simultaneous control agent and a control method using the same.

Crop damage caused by plant diseases is a huge economic loss, amounting to about 200 trillion won in the world. To prevent this, excessive use of chemical pesticides causes environmental pollution and toxin toxicity. You are in crisis.

In recent years, the area of plant cultivation of vegetables, fruits, and flowers has been increasing, but in Korea, most of the small-scale small-scale farmers focus on production aiming at high prices in the short term. Due to the lack of ventilation due to the high humidity conditions and abnormal temperature phenomena in the facility due to poor ventilation due to the lack of cultivation of crops, a lot of diseases occur, resulting in a decrease in quantity and quality. These major diseases have been reported as gray mold, powdery mildew, late blight, and wilted disease.

In particular, gray mold and powdery mildew ( Sphaerotheca humuli ) caused by Botrytis cinerea are the most damaging. Asymptomatic disease has a wide host range and is one of the major diseases such as vegetables, apples and grapes such as strawberries, tomatoes and cucumbers. The gray mold disease occurs when the temperature is relatively low and high humidity is maintained, and it begins to develop from dead plants or deteriorated areas, and once developed, the disease progresses even if it is not at the onset temperature of the pathogen. The control of gray mold disease is made of resistant varieties, seedling control by cultivation environment control and diseased plant removal, chemical control using fungicides. However, resistance to varieties and seedlings have little effect. As a result, ash control is mainly dependent on chemical control. Aseptic fungicides for gray mold disease are used, but procmidone, iprodione, and benomil are used, but resistance to these drugs has started to appear, and the control effect is greatly reduced.

Powdery mildew is the most common disease in facility cultivation and open field cultivation. It is a trend that the occurrence of powdery mildew is increasing due to the low humidity while keeping the environment dry while the facilities are heated. In order to control the powdery mildew of strawberry caused by spheroteca humulley, the chemicals were controlled by sterol biosynthesis inhibitor, beta tubulin binding inhibitor, and cytochrome in the mitochondrial electron transfer system, showing bactericidal activity. However, the continuous use of such fungicides caused drug resistance, which caused difficulties in controlling powdery mildew, and suffered from problems such as direct toxicity and retention of toxicity due to continuous use and abuse of chemical pesticides. As a result, the development of new environmentally friendly disinfectants is urgently required.

In the past, attempts to control plant diseases without the use of chemical pesticides have been made in various ways, and at the present time, the demand for high-quality safe agricultural products is increasing. Development of traditional farming methods is emerging as the only alternative. Recently, biological control using plant resources, such as medicinal plants, for the major disease control of plants has been developed and used as an environmentally friendly agricultural technology. Many phytopathogenic strains have been studied for pathogenic fungi, bacteria, and nematodes, but among them, medicinal plant yellow lotus root is used to control gray mold and powdery mildew disease that cause the most damage in strawberry facilities. There is no report.

The present invention has been made to solve the problems of the prior art as described above, the main object is to spray on the leaf rights of the crops to suppress the development of the crop growth period, as well as to significantly increase the number of crops, strawberries It is effective in suppressing gray mold and powdery mildew that occur frequently in the plant, and is a plant disease control system that can produce high value-added organic products by environmentally friendly control of fungal diseases that cause serious loss to strawberries that are intensively grown in the facility. In providing.

Another object of the present invention is not only to increase the number of crops significantly by inhibiting the growth of the crop growth period by spraying on the lobe of the crop, but also effectively suppress the gray mold disease and powdery mildew that occurs a lot of strawberries, especially in the facility The present invention provides a method of simultaneously controlling plant diseases that can produce high value organic products by environmentally controlling fungal diseases that cause serious losses to cultivated strawberries.

The technical problem of the present invention as described above is achieved by the following means.

(1) Simultaneous control of plant diseases, including strawberry gray mold and powdery mildew, which contain the root of the yellow lotus or its extract as an active ingredient.

(2) The method of claim 1, wherein the extract of the Rhododendron root is extracted by adding ethanol to the root of the Rhododendron, or by adding water to the root of the Rhododendron and extracting the mixture by boiling water reflux at 80 to 120 ° C. for 2-3 days. Centrifugation of one solution to extract the concentrated supernatant under reduced pressure, plant co-controlling diseases including strawberry gray mold and powdery mildew, characterized in that it further comprises sulfur and natural soap as an auxiliary component.

(3) The plant disease simultaneous control agent according to the item 2, wherein the strawberry gray mold and powdery mildew are liquefied by adding alginic acid.

(4) A method of controlling the plant diseases including strawberry gray mold and powdery mildew by treating the strawberry with the control agent according to any one of items 1 to 3 at the same time.

The control agent according to the present invention can not only increase the yield of crops by inhibiting the growth of the crop growth period by spraying on the lobe of crops, but also effectively suppresses gray mold and powdery mildew, which occur in strawberries, especially in the facility. Environmentally friendly control of fungal diseases that cause severe losses to intensively grown strawberries enables the production of high value-added organic produce.

Hereinafter, the content of the present invention will be described in more detail.

Coptis chinensis according to the present invention is a medicinal plant that is a perennial herb that grows or grows in China, Japan, India, Nepal, Korea, etc., and its root is mainly composed of berberine belonging to a quaternary base. It contains palmatine, Coptisine, Warenine, Picrorhizin, Tanin, and resins. These components include Micrococcus pyrogens, Alternaria sp., Penicillium sp., Aspergillus sp. It has strong antibacterial activity even at low concentrations, and increases the preservation effect of cosmetics. In China, it has been used to preserve noodles for a long time. In addition, patents have been reported for treating cow mastitis by Staphylococcus aureus , treating diseases by Clostridium perfringen , promoting hair growth and anticancer agents.

According to the present invention, the medicinal plant Huang Lian having such a function is a plant pathogen and has the ability to control causative bacteria such as gray mold, powdery mildew, which are the main diseases of crops. In particular, spore germination inhibitory ability of the extract of the present invention against strawberry gray mold and powdery mildew bacteria is superior to the antagonistic ability against other plant pathogens.

The plant disease control agent of the present invention contains as an active ingredient a yellow lotus root extract capable of controlling plant diseases as described above. In the plant disease control agent of the present invention, although the medicinal plant can be used as the medicinal plant itself, a mixed liquid containing the active ingredient and auxiliary components extracted from the root of the medicinal plant can also be used. The active ingredient of the Rhododendron root extract has the taxonomic characteristics of the Rhododendron, has the effect of controlling plant diseases, and extracts using heat or a solvent, and also the rhubarb root extract mixed with auxiliary component solutions are also available. Therefore, the yellow lotus root contained in the plant disease control agent of the present invention also includes a mixed solution (water) containing the active ingredient of the yellow lotus root extract.

The yellow lotus root extract according to the present invention may be ethanol, water or a mixture of two extracts thereof. Preferably, 70% to 100% ethanol, which is 3 to 5 times the dry weight of the yellow barley root, is added and refluxed, and the filtered solution is centrifuged to separate only the supernatant and concentrated under reduced pressure. It may be an extract obtained by adding 3 to 10 times of water and extracting with a boiling water reflux apparatus at 80 to 120 ° C. for 2 to 3 days, followed by centrifugation to concentrate the supernatant under reduced pressure. At this time, it is preferable to go through the process of filtration by extracting with a boiling water reflux device and then poured into filter paper or three-ply gauze.

Preferably, when the mixture extract of the ethanol and water extract, these mixing ratio is preferably in the range of 40:60 ~ 60:40. In the mixed composition range, a simultaneous control effect against various diseases is expected.

In this specification, "control" shall be used by the meaning including prevention and avoidance of a disease and a pest, as well as removal and death. Therefore, even if the plant is already infected with the pathogen, the pathogen can be removed from the plant by applying the plant disease control agent of the present invention, thereby preventing the onset and worsening of the pathogen.

In the plant disease control agent according to the present invention, it is preferable that the sulfur extract extract contains 16 to 30% by weight (solid content: based on 10 mg / ml) based on the total weight of the plant disease control agent. In addition, in the case of using the mixture of the rhubarb extract and the auxiliary component, the application time and application amount may be appropriately determined based on the above concentration.

In addition, in the plant disease control agent according to the present invention, the extract or mixture of the roots of the rhubarb may be used alone, or diluted with an inert liquid or solid carrier and used as a medicament to which a surfactant or other auxiliary agent is added if necessary. good. Specific formulation examples include formulations such as granules, powders, hydrating agents, and emulsions.

Examples of preferred carriers include talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, porous solid carrier, water, isopropyl alcohol, xylene, cyclohexanone, methylnaphthalene, alkylglycol, Liquid carriers, such as a natural soap, etc. are mentioned. As surfactant and a dispersing agent, for example, dinaphthyl methane sulfonate, alcohol sulfate ester salt, alkylaryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkylaryl ether, polyoxyethylene sorbitan monoal Chelates; and the like. Carboxymethyl cellulose, polyethylene glycol, propylene glycol, gum arabic, xanthan gum etc. are mentioned as an adjuvant, Alginic acid, skim milk, pH buffer, etc. are mentioned as a protective agent. In this case, the amount of the yellow lotus root extract and / or the amount of the active ingredient, the application time and the application amount can be appropriately determined according to the case of the effective concentration.

In addition, the plant disease control agent of the present invention may include other components such as insecticides, other fungicides, herbicides, plant growth regulators, fertilizers, minerals, etc. as necessary as an active ingredient. In addition, the plant disease control agent of the present invention may contain other kinds of plant extracts simultaneously with the sulfur extract. Preferably, the extract may further include the bark extract and / or the cheongung extract, wherein each extract or mixed extract supplements the additional control activity even after the control activity is finished, while continuing the simultaneous control activity exerted by the Rhododendron root extract. It is preferable because it can be done. The process of obtaining these extracts can be obtained through the same process as that of the root extract of the yellow lotus, the content of which can be added in an amount of 1 to 10% by weight based on the total weight. If added in less than 1% by weight can not be expected as long as the persistence and auxiliary activity of the bactericidal effect, exceeding 10% by weight may affect the growth of strawberries is not preferred.

The plant disease control agent according to the present invention may further include sulfur as an auxiliary component. At this time, sulfur is a natural organic sulfur, one of the nonmetallic elements, which is composed of yellow crystals at room temperature, and has excellent antibacterial and bactericidal properties. In particular, when added in a predetermined ratio with the rye root extract can improve the control activity against various strawberry diseases. Sulfur is preferably added in the range of 5 to 15% by weight based on the total weight, if less than 5% by weight it is difficult to improve the control activity against various strawberry diseases, if exceeding 15% by weight rather strawberry It is undesirable because there is a risk of adversely affecting the growth of.

The plant disease control agent of the present invention may be used directly as it is, or diluted with water or the like. The method of using a plant disease control agent is not specifically limited, For example, the method of directly spreading on a plant or host plant, the method of irrigation to soil, the method of adding to the water or fertilizer added to a plant or soil, etc. are mentioned. Since the dosage of other formulations varies depending on the target disease, the target crop, the method of application, the tendency of occurrence, the degree of damage, the environmental conditions, the formulation to be used, etc., it is preferable to adjust them appropriately.

The control agent according to the present invention was found to have a control effect of 62% against gray mold and 52% of powdery mildew disease as a result of treating the leaves of strawberry, and the simultaneous control activity was very excellent.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following illustrated examples are only presented to aid the understanding of the present invention and should not be construed as limiting the scope of the present invention.

Example 1

160 g of rye roots were ground and ground into 1 liter of 70% ethanol. The mixture was stirred for 4 hours at 150 rpm at room temperature with a reflux extractor, and then filtered using a filter paper. Thereafter, the filtrate was centrifuged, and only the supernatant was concentrated under reduced pressure to obtain a yellow root extract. The resulting extract was then used while stored at 4 ° C.

[Example 2]

160 g of rye roots were ground, 1 liter of water was added thereto, extracted with a boiling water reflux apparatus at 80 ° C. for 2 days, and filtered using a filter paper. Thereafter, the filtrate was centrifuged, and only the supernatant was concentrated under reduced pressure to obtain a yellow root extract. The resulting extract was then used while stored at 4 ° C.

[Example 3]

16-30% by weight of the concentrated concentrate (solid content: 10mg / ml), 12.5% by weight of natural soap, 15% by weight of natural organic sulfur, the concentrated condensate obtained from Example 1 and Example 2 was concentrated under reduced pressure, 45 wt% of alginic acid was added as an electrodeposition agent to liquefy to prepare a strawberry disease co-control agent (see the composition of Table 1).

Raw material composition H16 H20 H24 goldthread 16% 20 24 Natural Soap 12.5 12.5 12.5 sulfur 15 15 15 Electrodeposition Agent (Alginic Acid) 45 45 45 water Balance Balance Balance

Experimental Example 1 Strawberry Gray Mold Fungus Packaging Control Effect

The control effect of Rhizome root extract against strawberry gray fungus disease was investigated by ingredient. The raspberry varieties were sprayed at 7-day intervals from 14 days before the first flowering by the concentration of the yellow rye root extract, and were treated 1 ~ 3 times by the number of sprays, and the leaves were treated with foliage and the spraying concentration was 500 times and 1000 times. . As a result, 62.3% of the control effect was obtained against strawberry gray mold (Table 2, Table 3). The control efficiency (%) was expressed as {(non-treatment cure rate-specific cure rate for each treatment)} / non-treatment cure rate × 100.

Strawberry gray mold packaging control effect (500 times) process Disease rate (%) Prevention efficiency (%) 1 time Episode 2 3rd time 1 time Episode 2 3rd time H16 26.8 24.4 20.5 48.2 53.4 58.9 H20 25.2 21.0 19.0 51.4 59.7 61.9 H24 22.2 25.5 22.6 57.2 51.2 54.7 No treatment 51.8 52.3 49.9 - - -

Strawberry gray mold packaging control effect (1,000 times) process Disease rate (%) Prevention efficiency (%) 1 time Episode 2 3rd time 1 time Episode 2 3rd time H16 33.3 29.5 26.0 43.9 47.6 48.0 H20 30.6 28.9 25.6 48.6 48.6 48.8 H24 30.2 28.6 24.0 49.2 49.2 51.9 No treatment 59.4 56.3 50.0 - - -

Experimental Example 2 Strawberry Powder Disease Packaging Control Effect

The control effect of the Rhizome root extract against strawberry powdery mildew was investigated by ingredient. Strawberry rye varieties were treated three times at 10-day intervals after coating with the concentrations of the yellow lotus root extract, and the method of spraying was applied to the foliage, and the spraying concentration was 500 times and 1000 times. Irradiation period was investigated 10 days after the final application. As a result, 51.7% of the control effect against strawberry powdery mildew was obtained (Table 4). The control efficiency (%) was expressed as {(non-treatment cure rate-specific cure rate for each treatment)} / non-treatment cure rate # 100.

Strawberry powdery packaging effect process Disease rate (%) Prevention efficiency (%) 500 times 1,000 times 500 times 1,000 times H16 30.7 27.8 48.7 47.8 H20 29.3 26.8 51.0 49.7 H24 29.0 26.2 51.7 50.8 No treatment 59.9 53.2 - -

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It can be understood that

Claims (4)

To the mixture of concentrated extract (solid content: 10mg / ml), 16 ~ 24% by weight of natural soap, 12.5% by weight of natural soap, 15% by weight of natural organic sulfur, 45% by weight of alginic acid and the remaining amount of water were added as a electrodeposition agent. Simultaneous control of plant diseases, including strawberry gray mold and powdery mildew, characterized in that liquefied by. The method of claim 1, wherein the extract of the Rhododendron root is extracted by adding ethanol to the root of the Rhododendron, or by adding water to the root of the Rhododendron and extracting the mixture by boiling water reflux at 80 to 120 ° C. for 2 to 3 days. Simultaneous control of plant diseases, including strawberry gray mold and powdery mildew, characterized in that the extract was concentrated under reduced pressure by centrifugation. delete The control method of claim 1 or 2, wherein the control agent according to claim 1 is treated with strawberries to control plant diseases including strawberry gray mold and powdery mildew.