CN106818931A - The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse - Google Patents
The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse Download PDFInfo
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- CN106818931A CN106818931A CN201710102078.8A CN201710102078A CN106818931A CN 106818931 A CN106818931 A CN 106818931A CN 201710102078 A CN201710102078 A CN 201710102078A CN 106818931 A CN106818931 A CN 106818931A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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Abstract
The invention discloses a kind of biological pesticide suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse, belong to biological pesticide technical field.The biological pesticide is made up of effective ingredient component A, component B and surfactant, cosolvent compounding.The effective ingredient component A is made up of the Chinese herbal medicine of following weight portion：200 240 parts of ajuga decumbens, 180 200 parts of fine stern wind, 300 360 parts of Herba Cyperi Glomerati.Proved by field test, not only preventive effect is good for biological pesticide of the present invention, and residues of pesticides are few, and can improve the quality of cucumber.
Description
Technical field
The present invention relates to preventing and treating cucumber bacterial angular leaf spot in agricultural technology field, more particularly to a kind of day-light greenhouse
Chemical biological pesticide.
Background technology
Cucumber is a kind of plant of Curcurbitaceae Cucumis, and in some areas, cucumber is also referred to as green cucumber and cucumber.As most
One of agricultural product on numerous common people's dining table are often appeared in, cucumber is distributed widely in each area of China.The cucumber temperature of modernization
Room high-yield culture technique allows the cucumber to become more welcome, while the common people's growing life requirement is met, cucumber
Quality problems also allow people to worry.Now, the key factor of influence cucumber quality is exactly quality and Pesticide Residue, this with it is extensive
There is direct relation using traditional chemical agricultural chemicals, for a long time, largely the ecological balance is seriously damaged using chemical pesticide.
Especially in day-light greenhouse, chemical pesticide is easy to accumulation, and volatilization is slow, easily incorporates soil, polluted underground water；Chemical synthesis agriculture
Medicine grain, melon fruits and vegetables and herbage surface residual quantity it is many, the holdup time is long, be not easily decomposed, to people, animal, the health of fowl
Cause serious harm.Therefore, we are in green cucumber for many years, continuous summing up experience makes full use of China's abundant
Chinese herbal medicine resource, develops the chemical biological pesticide suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse.
The content of the invention
It is an object of the invention to provide a kind of chemistry suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse
Biological pesticide.
The purpose of the present invention is achieved through the following technical solutions, and biological pesticide of the present invention is by effective ingredient
Component A, component B and surfactant, cosolvent compounding are made；The effective ingredient component A is by the medium-height grass of following weight portion
Medicine is made：Ajuga decumbens 200-240 parts, 180-200 parts, Herba Cyperi Glomerati 300-360 parts of fine stern wind.
Preferably：220 parts of ajuga decumbens, 190 parts of fine stern wind, 330 parts of Herba Cyperi Glomerati.
Preparation method：Each Chinese herbal medicine is smashed and is mixed, poured into container, 80% ethanol of addition, ultrasonic oscillation 25min,
Depressurized suction filtration twice, the solution clarified, 40 DEG C of rotary evaporations, is steamed to volume and is no longer changed, in 50 DEG C of baking ovens
Drying, pulverizes last, adds distilled water to be settled to 0.55kg/L, obtains final product this biological pesticide.
The component B is 50% first frost copper wettable powder, and the compounding weight ratio of the component A and component B is 4: 1.
The surfactant is calcium dodecyl benzene sulfonate, and cosolvent is cyclohexanone, and its addition is respectively the present invention
The 3% of biological pesticide weight and 5%.
Fang Zhong：From hair selfheal is relieving cough and reducing sputum, clearing heat and cooling blood, subdhing swelling and detoxicating, to staphylococcus aureus, micrococcus catarrhalis,
Pneumococcus, alpha streptococcus, Escherichia coli etc. have good inhibiting effect；Fine stern wind expelling wind and clearing away cold, promoting blood circulation sterilization, have
Certain bacteriostasis；Herba Cyperi Glomerati is relieving cough and reducing sputum, antibacterial, to Bacillus influenzae, alpha streptococcus, Nai Shi diplococcuses, tetrads
Deng with good inhibiting effect.
Specific embodiment
Implementation method one：Biological pesticide of the present invention is by effective ingredient component A, component B and surfactant, helps
Solvent complex is made；Weigh ajuga decumbens 200g, fine stern wind 180g, the Herba Cyperi Glomerati 300g in component A；Each Chinese herbal medicine is smashed
Mix, pour into container, 80% ethanol of addition, ultrasonic oscillation 25min, depressurized suction filtration twice, is clarified
Solution, 40 DEG C of rotary evaporations steam to volume and no longer change, and are dried in 50 DEG C of baking ovens, pulverize last, add distilled water constant volume
To 0.55kg/L, this biological pesticide component A is obtained final product；The ratio by weight 4: 1 adds component B again, i.e. 50% first frost copper is wettable
Property pulvis and appropriate calcium dodecyl benzene sulfonate, cyclohexanone, obtain final product biological pesticide of the present invention.
Implementation method two：Biological pesticide of the present invention is by effective ingredient component A, component B and surfactant, helps
Solvent complex is made；Weigh ajuga decumbens 200g, fine stern wind 180g, the Herba Cyperi Glomerati 300g in component A；Preparation method is with implementation
Mode one.
Implementation method three：Biological pesticide of the present invention is by effective ingredient component A, component B and surfactant, helps
Solvent complex is made；Weigh ajuga decumbens 200g, fine stern wind 180g, the Herba Cyperi Glomerati 300g in component A；Preparation method is with implementation
Mode one.
Bacteriostatic test
Strains tested：Pseudomonas syringae cucumber bacterium angular leaf spot pvs oryzae and oryzicola Pseudomonas syringae
pv.lachryrnans(PSL-8).Cucumber variety：Chang Chun Mi Ci.Chemical agent：50% first frost copper wettable powder.
Test method
The biological pesticide being made by embodiment of the present invention two is set to A medicine groups by us, only will use ajuga decumbens single
Medicine is set to B medicine groups by the biological pesticide that the preparation method of embodiment of the present invention two is made；Will be only with fine stern wind simple by this
The biological pesticide that the preparation method of invention embodiment two is made is set to C medicine groups；Will be only real by the present invention with Herba Cyperi Glomerati simple
Apply the biological pesticide that the preparation method of mode two is made and be set to D medicine groups；50% first frost copper wettable powder chemical agent group is E medicines
Group；Clear water group is CK.
Experiment measure is carried out according to conventional bacteriostatic test method, the bacteria suspension 2ml that concentration is 108cfu/ml is taken, added
In 200ml KB culture mediums (40 DEG C), it is poured into the culture dish of diameter 30cm, after solidification with the card punch of 4mm on flat board
14 holes are made a call to, after each group liquid is diluted, 100ul is taken respectively with pipettor, be separately added into each hole, sterilized water control, 4
Secondary repetition, is placed in 28 DEG C of incubators and cultivates, and the size that inhibition zone is measured after 48h is as shown in the table：
It is the experimental result for measuring in table
| Medicament | Diluted concentration (mg/L) | Inhibition zone size (mm) |
| A | 3000 | 20.80 |
| B | 3000 | 11 |
| C | 3000 | 12 |
| D | 3000 | 13 |
| E | 120 | 20 |
| CK | 0 |
Tested more than, it is antibacterial although each biological pesticide group preparation method, diluted concentration, consumption are all identical
Effect is dramatically different, and A groups fungistatic effect preferably, illustrate the synergistic function of generation after each simple compatibility in A groups, antibacterial
Effect is best.
Field test
Above each group liquid is diluted 350 times by us respectively below, carries out field medicine to cucumber bacterial angular leaf spot respectively
Effect experiment, so that beneficial effects of the present invention are better described.
Linyi City Yinan County green cucumber ground is experimental field located at, cucumber bacterial angular leaf spot, first 5 days of experiment and experiment has been sent out
During do not apply other agricultural chemicals.Experimental field soil property is medium, and rich water is medium, and management is consistent.Temperature is taken the photograph 22~31 during experiment
Family name's degree, 27 degrees Celsius of mean temperature；6 treatment are set in this field test.Each treatment is repeated 2 times, totally 12 treatment cells, choosing
Select growing way and the basically identical field of morbidity originates the 1st medication of phase in cucumber bacterial angular leaf spot, hereafter every 15d medications 1 time,
Continuous 2 times, using knapsack sprayer, amount of liquid medicine standard is 35kg/ mus, and blade and stem are uniform by medicine, it is to avoid liquid drifts about.
Second medicine is applied after being spaced 15 days after first time dispenser, common dispenser is twice.Proceed by investigation within 7 days after dispenser, take take at 5 points
Sample method, pinpoints adventive, determines the method for the indefinite leaf of the number of sheets, and every is investigated 18 blades (bottom 6,6, middle part, 6, top),
The classification investigation state of an illness, counts the incidence of disease and disease index, calculates prevention effect.
Severity Scaling standard：0 grade, disease-free spot on leaf；1 grade, lesion area accounts for whole leaf area below 1/4；2 grades, scab
Area accounts for the 1/4~1/2 of whole leaf area；3 grades, lesion area accounts for the 1/2~3/4 of whole leaf area；4 grades, lesion area is accounted for
More than the 3/4 of whole leaf area.
Disease index (%)=∑ (number of blade of the sick level × sick level) × 100/ highest disease level × total leaf number.
Prevention effect (the %)=control disease index of (control disease index-treatment disease index) × 100/.
Experimental result：Different disposal see the table below to the prevention effect of cucumber bacterial angular leaf spot
As seen from the above table, twice after dispenser, although each biological pesticide group preparation method, diluted concentration, consumption are all identical,
Prevention effect is dramatically different, and A groups prevention effect preferably, Synergistic work is generated after illustrating each simple compatibility in A groups
With significantly improving field control effect.
Experimental result：Influence of the different disposal to cucumber quality see the table below (per the content of 100g nutriments)
As seen from the above table, the cucumber for being grown using biological pesticide of the present invention, not only nutritional ingredient be better than other each medicine groups,
It is better than chemical drugs group, and residues of pesticides are up to standard, exceed our expectations is that Storage period is obviously prolonged, it is to avoid unnecessary corruption
It is rotten, it is readily transported storage.
In experiment more than, more the good news is biological pesticide solution of the present invention is prevented leaf folder, aphid with good
Control effect.Therefore, specially be diluted for biological pesticide of the invention by we, extension rate is 350 times.Make for greengrocery
The prevention and control of plant diseases, pest control on thing, fruit tree kind, medicinal material, flowers tealeaves and meadow, vaporific uniform complete stool sprays.
The pest control effect such as following table of chemical biological pesticide of the present invention
In sum：Biological pesticide medicine speed of the invention plays fast, and drug effect is long, and after spraying 1 day, insect inhibiting rate is 90%
More than, after 5 days, insect inhibiting rate more than 96%, based on formula of tagging inhaled supplemented by killing, and insect does not produce anti-by the biological pesticide
The property of medicine.
Experiment more than, prevention effect, the nutritional ingredient of cucumber, storage of the A groups to cucumber bacterial angular leaf spot
Phase, pest controling effect are deposited significantly better than other each groups, that is to say, that each simple and list in biological pesticide of the invention
After there is synergistic function between taste medicine and chemical pesticide, to improve product quality is conducive to, the direction of residues of pesticides is eliminated
Development, achieves satisfied experiment effect, is worthy of popularization.
Claims (5)
1. it is a kind of suitable in day-light greenhouse prevent and treat cucumber bacterial angular leaf spot biological pesticide, it is characterised in that the biology
Agricultural chemicals is made up of effective ingredient component A, component B and surfactant, cosolvent compounding.
2. a kind of biological agriculture suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse as claimed in claim 1
Medicine, it is characterised in that the effective ingredient component A is made up of the Chinese herbal medicine of following weight portion：Ajuga decumbens 200-240 parts,
180-200 parts, Herba Cyperi Glomerati 300-360 parts of fine stern wind.
3. a kind of biological agriculture suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse as claimed in claim 1
Medicine, it is characterised in that the effective ingredient component A is made up of the Chinese herbal medicine of following weight portion：220 parts of ajuga decumbens, fine stern
190 parts of wind, 330 parts of Herba Cyperi Glomerati.
4. a kind of biological agriculture suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse as claimed in claim 1
Medicine, it is characterised in that the component B is 50% first frost copper wettable powder, and the compounding weight ratio of the component A and component B is 4:
1。
5. a kind of biological agriculture suitable for preventing and treating cucumber bacterial angular leaf spot in day-light greenhouse as claimed in claim 1
Medicine, it is characterised in that the surfactant is calcium dodecyl benzene sulfonate, and cosolvent is cyclohexanone, its addition is respectively this
The 3% and 5% of invention biochemical pesticides weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710102078.8A CN106818931A (en) | 2017-02-15 | 2017-02-15 | The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710102078.8A CN106818931A (en) | 2017-02-15 | 2017-02-15 | The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse |
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| Publication Number | Publication Date |
|---|---|
| CN106818931A true CN106818931A (en) | 2017-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710102078.8A Pending CN106818931A (en) | 2017-02-15 | 2017-02-15 | The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101911956A (en) * | 2010-08-18 | 2010-12-15 | 山西大学 | Plant source pesticide and production method and application thereof |
| CN102715198A (en) * | 2012-06-15 | 2012-10-10 | 米如钦 | Traditional Chinese herbal medicine mixture for preventing and controlling cucumber bacterial angular leaf spot |
| CN102805112A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Botanical pesticide for controlling cucumber bacterial angular leaf spot |
| CN104397076A (en) * | 2014-11-23 | 2015-03-11 | 青岛锐志农业技术服务有限公司 | Environment-friendly plant pesticide for preventing and controlling crop insect pests |
| CN105123803A (en) * | 2015-10-26 | 2015-12-09 | 唐睿 | Botanical fungicide containing thymol and kasugamycin |
| CN105638330A (en) * | 2015-12-14 | 2016-06-08 | 高阳 | Method for preventing and controlling cucumber bacterial leaf blight on basis of biological experiment class |
| CN105724130A (en) * | 2016-01-28 | 2016-07-06 | 高贤泽 | Method for controlling cucumber bacterial angular leaf spots based on biology experiments for senior high school |
-
2017
- 2017-02-15 CN CN201710102078.8A patent/CN106818931A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101911956A (en) * | 2010-08-18 | 2010-12-15 | 山西大学 | Plant source pesticide and production method and application thereof |
| CN102805112A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Botanical pesticide for controlling cucumber bacterial angular leaf spot |
| CN102715198A (en) * | 2012-06-15 | 2012-10-10 | 米如钦 | Traditional Chinese herbal medicine mixture for preventing and controlling cucumber bacterial angular leaf spot |
| CN104397076A (en) * | 2014-11-23 | 2015-03-11 | 青岛锐志农业技术服务有限公司 | Environment-friendly plant pesticide for preventing and controlling crop insect pests |
| CN105123803A (en) * | 2015-10-26 | 2015-12-09 | 唐睿 | Botanical fungicide containing thymol and kasugamycin |
| CN105638330A (en) * | 2015-12-14 | 2016-06-08 | 高阳 | Method for preventing and controlling cucumber bacterial leaf blight on basis of biological experiment class |
| CN105724130A (en) * | 2016-01-28 | 2016-07-06 | 高贤泽 | Method for controlling cucumber bacterial angular leaf spots based on biology experiments for senior high school |
Non-Patent Citations (1)
| Title |
|---|
| 吕勤 等编: "《新编农药使用技术》", 31 January 2008, 广西科学技术出版社 * |
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Application publication date: 20170613 |