CN105532663A - Biological agent for controlling gray molds by being sprayed on flowers - Google Patents
Biological agent for controlling gray molds by being sprayed on flowers Download PDFInfo
- Publication number
- CN105532663A CN105532663A CN201610096437.9A CN201610096437A CN105532663A CN 105532663 A CN105532663 A CN 105532663A CN 201610096437 A CN201610096437 A CN 201610096437A CN 105532663 A CN105532663 A CN 105532663A
- Authority
- CN
- China
- Prior art keywords
- epsilon
- polylysine
- agent
- drop
- host
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological agent for controlling gray molds by being sprayed on flowers. The biological agent contains epsilon-polylysine. According to one composition, epsilon-polylysine serves as a main agent and is added into tomatotone which is sprayed on plant flowers. According to the other composition, epsilon-polylysine serves as a main agent, and an auxiliary agent is selected from the organic compound chitosan or the inorganic compound ferrous sulfate. The main agent and the auxiliary agent of the biological agent have a remarkable synergistic effect, and therefore resistance to and control over the gray molds on plant fruits and leaves can be effectively enhanced.
Description
Technical field
The present invention relates to a kind of biologic product preventing and treating gray mold, belong to agro-ecology pesticide field.
Background technology
Epsilon-polylysine is the polymer be formed by connecting after α-form amido link between carboxyl and epsilon-amino by a twenty or thirty lysine molecule, it is a kind of homogeneous polyamino acid, extensive research for many years confirms that it has bacteriostasis efficacy, and this characteristic makes it be widely applied in food antiseptic field as preservative.
Botrytis cinerea infects fruit and mainly relies on residual petal and column cap, promotes removal of petal and add the epsilon-polylysine with good preventive effect in drop-proof agent spraying decoration liquid, is block the effective way infected.
Research in recent years confirms that the growth of epsilon-polylysine to multiple mould has certain inhibitory action, in flat board face-off, be used alone epsilon-polylysine show ideal bacteriostasis rate, therefore also have some research to attempt being applied to the control of the various gray mold in field, but test display application its preventive effect after crops does not reach ideal effect.This area have developed some compound formulations for this reason, a kind of research direction is mated with multiple alkaloid it, the preventive effect of epsilon-polylysine is improved by alkaloidal antipathogen and killing fungus effect, another kind of research direction is using epsilon-polylysine as auxiliary material, and collocation has the mantoquita of good killing fungus activity traditionally to improve the effect of its killing fungus.
But above-mentioned both direction achievement in research all shows, even if consider above-mentioned synergy, its preventive effect being used for preventing and treating graw mold of tomato is also only about 80%, still has huge room for improvement.
Summary of the invention
Applicant have extensively studied the effect that epsilon-polylysine prevents and treats gray mold in reality plantation, by the further investigation of the molecular biological roles mechanism on gene expression level, added in the drop-proof agent of plant spraying decoration, or itself and organic compound (shitosan) or inorganic compound (ferrous sulfate) are carried out the control efficiency of composite raising to gray mold.
Specifically, the present invention is achieved through the following technical solutions:
Spraying decoration prevents and treats a biologic product for gray mold, and these compositions all comprise epsilon-polylysine, consists of one of following: be 1. host with epsilon-polylysine, add in the drop-proof agent of plant spraying decoration; 2. be host with epsilon-polylysine, auxiliary agent selects organic compound respectively: shitosan, or inorganic compound: ferrous sulfate.
Due to the difference of biological chemistry action mechanism, above-mentioned two kinds of composition form applicabilities are each variant, and 1. combining form is applicable to spraying decoration; 2. combining form is applicable to spraying application to plant leaf blade.
In above-mentioned disclosed combining form, the consumption of host, auxiliary agent can adjust according to actual needs, and the amount ratio of the two is within the scope of 1: 1-10 usually.
According to the difference of type of compounds, specifically can adjust consumption, be preferably following usage ratio:
Be host with epsilon-polylysine, add in the drop-proof agent of plant spraying decoration, the amount ratio of epsilon-polylysine and drop-proof agent is 1: 1-2, preferably 1: 1.
Take epsilon-polylysine as host, selection of auxiliary organic compound: shitosan, wherein the amount ratio of host and auxiliary agent is 1: 1-2, is preferably respectively 1: 2.
Take epsilon-polylysine as host, selection of auxiliary inorganic compound: ferrous sulfate, wherein the amount ratio of host and auxiliary agent is 1: 4-10, is preferably respectively 1: 4.44.
Be host with epsilon-polylysine, add in the drop-proof agent of plant spraying decoration, with this combination drug, spraying decoration process is carried out to tomato plant, the flowering rate of plant can be increased, thus reduce sick fruit number.
Take epsilon-polylysine as host, organic compound or inorganic compound are auxiliary agent, significantly can strengthen the induction of epsilon-polylysine to resistance-related enzymes on tomato plant or protein gene, as β-1,3-dextranase, chitinase, PR albumen etc., gene expression amount was raised in the different time periods, and these inducible gene expressions enhance the resistance of plant to gray mold, thus improve the control efficiency of gray mold.
Applicant have extensively studied the synergy of different agents and epsilon-polylysine, find relative to other composition, epsilon-polylysine is added in the drop-proof agent of plant spraying decoration, realize control efficiency completely to gray mold, therefore the combined pharmaceutical composition preventing and treating gray mold of the present invention preferentially selects epsilon-polylysine+drop-proof agent.
In the present invention, alleged epsilon-polylysine, refers to crosslinked and noncrosslinking lysine polymers or oligomer, preferably adopts the epsilon-polylysine with 3600 ~ 4300g/mol average molar mass (weight average).The amino of the basic lysine unit of composition epsilon-polylysine can be connected by α and/or ε position, and normally ε position connects.
In the present invention, alleged gray mold is the multiple fungal disease of a kind of frequently seen plants, is more common in the fruit trees and vegetables such as tomato, cucumber and strawberry, especially tomato and cucumber.
In the present invention, it will be appreciated by those skilled in the art that when by composition embody rule, need to be dissolved in solvent and spray with rational concentration, most preferred solvent is water, but other aromatic solvent, alcohols, ketone etc. are also available, spray in solution what configure, solvent normally 10 ~ 95%, according to the planting density of plant, spray according to the consumption of 30 ~ 50g/ mu.
In the present invention, those skilled in the art's easy understand is for the ease of the sale of product and production, above-mentioned composition can be prepared as corresponding preparation type as required, as water-soluble granular, emulsion, suspension, water-dispersible granule, water dispersible and water-soluble powder etc., these preparation types are all types common on agricultural chemicals, and it will not go into details herein.
Applicant carries out test display, and combined pharmaceutical composition of the present invention has ideal effect to preventing and treating gray mold, demonstrates epsilon-polylysine and creates syneryistic effect significantly.
Embodiment
In following enforcement, applicant provide some specific implementations of technical solution of the present invention, so that the effect of combined pharmaceutical composition of the present invention to be described.The following enforcement provided is only schematic, and those skilled in the art still belong to protection scope of the present invention understanding and grasp adjustment, replacement etc. that the basis of connotation of the present invention is carried out.
Embodiment 1
By epsilon-polylysine and drop-proof agent according to 1: 1 mass ratio mix, then add in 400 parts of water and be uniformly mixed.
Embodiment 2
By epsilon-polylysine and shitosan according to 1: 2 mass ratio mix, then add in 800 parts of water and be uniformly mixed.
Embodiment 3
By epsilon-polylysine and ferrous sulfate according to 1: 4.44 mass ratio mix, then add in 800 parts of water and be uniformly mixed.
In the above-described embodiments, the average molar mass (weight average) of epsilon-polylysine used is 3600 ~ 4300g/mol.
Preventing and treating the effect of gray mold in order to study epsilon-polylysine+drop-proof agent, applicant carried out following experiment.
Be host with epsilon-polylysine, add in the drop-proof agent of plant spraying decoration and carry out spraying decoration test, investigation drop-proof agent+epsilon-polylysine prevents and treats the effect of gray mold.At florescence of tomato plant according to the ratio spraying decoration of composite drug embodiment 1, singly to execute drop-proof agent and drop-proof agent+chemical pesticide for contrast, investigate disease fruit number and total really number respectively the ground floor fruit of plant and second layer fruit phase.Investigation result is as shown in table 1 below:
Table 1: drop-proof agent+epsilon-polylysine prevents and treats the effect of gray mold
The control rate of ground floor fruit: drop-proof agent+epsilon-polylysine 100%; Drop-proof agent+chemical pesticide 100%.
The control rate of second layer fruit: drop-proof agent+epsilon-polylysine 98.40%; Drop-proof agent+chemical pesticide 97.50%.
In order to study the induction to resistance-related enzymes on tomato plant or protein gene of epsilon-polylysine and organic compound or inorganic compound, adopt real time fluorescence quantifying PCR method research and β-1,3-dextranase, chitinase, PR protein related gene expression in the change of different time sections.
First extract the total serum IgE of tomato leaf, and detect its purity, concentration and integrality, then synthesize and detect the first chain cDNA, synthetic primer, adopt real-time fluorescence quantitative PCR to detect the expression change of each gene.Test arranges 3 process, A: singly execute embodiment 2-3 composite drug; B: spray embodiment 2-3 composite drug, induces after 3 days, inoculating tomato botrytis cinerea; C: singly connect tomato gray mould bacterium spore suspension.
Result of the test shows, spray epsilon-polylysine and organic compound or inorganic compound, inducing plant blade is after 3 days, spray pathogen, the GlucanaseA on tomato leaf can be made, GlucanaseB, Chitinase3, Chitinase9 and PR1b1 gene all obviously raises when 36h.And singly execute composite drug or singly execute pathogen spore suspension little on the impact of these gene expression amounts, illustrate that embodiment 2-3 composite drug can induce tomato leaf disease resistance, improve the control efficiency to graw mold of tomato by induction of resistance.
In order to study epsilon-polylysine and organic compound or the inorganic compound control efficiency to tomato plant gray mold, what first carry out is dull and stereotyped bacteriostatic test, and the test method of employing is In Vitro Bacteriostatic test method(s).
The pharmaceutical composition of Example 2-3, gradient dilution is carried out with sterile water, make amount to epsilon-polylysine concentration be 10,5,2.5,1.25,0.625,0.25, the PDA of 0.1g/L is dull and stereotyped, and set sterile water as blank, it is dull and stereotyped that positive control 80% procymidone 1000 times of dilutions make pastille PDA by above-mentioned identical diluted concentration.Be that the card punch of 4mm has the edge of the PDA flat board of the pathogen of Botrytis cinerea to beat to get bacterium cake, by pure culture biscuits involvng inoculation in above-mentioned pastille flat board long with diameter.Flat board is placed in 25 DEG C of heat insulating culture casees, cultivates 72h.Measure the clean growth diameter of bacterium colony by right-angled intersection method, calculate inhibiting rate.
Inhibiting rate=(the clean growth diameter of bacterium colony of the clean growth diameter-pastille of blank bacterium colony)/clean growth diameter × 100% of blank bacterium colony.
Result of the test shows, and the present composition all has strong inhibition effect to the growth of the pathogen of Botrytis cinerea, shows extract to the very significant fungistatic effect of the pathogen of Botrytis cinerea in concentration under being not less than 0.25g/L situation.Under the concentration of 0.25g/L, the bacteriostasis rate of embodiment 2-3 to the pathogen of Botrytis cinerea is respectively 91.63% and 91.04%, and the bacteriostasis rate of positive control is 51.56%.
On the basis of the above, applicant carried out potted plant efficiency test, before test medication, select the consistent potted tomato seedling of growing way, write tag number with permanent pen, insert in plant pot, discharge according to the order of sequence, be for experiment.
The pharmaceutical composition of embodiment 2-3 being diluted to 0.25g/L adopts spray-on process to carry out spray medicine to above-mentioned seedling, dries after spray medicine in greenhouse.
At the tomato plant true leaf surface uniform spray inoculation the pathogen of Botrytis cinerea spore suspension of marshalling, every porcelain dish (40 basin seedling) sprays bacterium liquid about 30ml, transparent plastic moisture preserving cover in porcelain dish upper cover, and carefully move in climatic cabinate, keep relative moisture 100%, temperature is 20 DEG C, plastics moisture preserving cover is removed after 24 hours, keep temperature 20 DEG C, humidity more than 85% (light application time in climatic cabinate: interlunation=1: 1), five days " Invest, Then Investigate "s, adopt the grade scale as following table 2 to carry out control efficiency evaluation:
Table 2: grade scale
Its control rate is respectively 85.49% and 76.38%.The control rate of positive control is 70.00%.
Claims (4)
1. spraying decoration prevents and treats a biologic product for gray mold, it is characterized in that comprising epsilon-polylysine, consists of one of following: be 1. host with epsilon-polylysine, add in the drop-proof agent of plant spraying decoration; 2. be host with epsilon-polylysine, auxiliary agent selects organic compound respectively: shitosan, or inorganic compound: ferrous sulfate.
2. biologic product according to claim 1, is characterized in that host is epsilon-polylysine, adds in the drop-proof agent of plant spraying decoration, and the amount ratio of epsilon-polylysine and drop-proof agent is 1: 1.
3. biologic product according to claim 1, is characterized in that auxiliary agent is shitosan, and the amount ratio of host and auxiliary agent is 1: 2.
4. biologic product according to claim 1, is characterized in that auxiliary agent is ferrous sulfate, and the amount ratio of host and auxiliary agent is 1: 4.44.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610096437.9A CN105532663A (en) | 2016-02-22 | 2016-02-22 | Biological agent for controlling gray molds by being sprayed on flowers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610096437.9A CN105532663A (en) | 2016-02-22 | 2016-02-22 | Biological agent for controlling gray molds by being sprayed on flowers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105532663A true CN105532663A (en) | 2016-05-04 |
Family
ID=55812666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610096437.9A Pending CN105532663A (en) | 2016-02-22 | 2016-02-22 | Biological agent for controlling gray molds by being sprayed on flowers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105532663A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730081A (en) * | 2019-02-26 | 2019-05-10 | 南京轩凯生物科技有限公司 | A kind of agriculture bactericide composition |
| CN111480498A (en) * | 2020-04-07 | 2020-08-04 | 浙江大学 | Method for preventing fruit gray mold by low-sulfur treatment before picking |
| CN113234766A (en) * | 2021-06-03 | 2021-08-10 | 淮北师范大学 | Biosynthesis method of epsilon-polylysine by adding microbial elicitor |
| CN113785842A (en) * | 2021-10-20 | 2021-12-14 | 海南大学 | Pesticide composition for preventing and treating plant fungal diseases and preparation method thereof |
| CN113875779A (en) * | 2021-10-20 | 2022-01-04 | 海南大学 | Bactericide composition and preparation method thereof |
| CN114732018A (en) * | 2022-03-22 | 2022-07-12 | 西北农林科技大学 | Biological agent, application and tomato gray mold prevention and treatment method |
| CN114938809A (en) * | 2022-04-29 | 2022-08-26 | 中国农业科学院植物保护研究所 | Compound composition of wuyiencin and epsilon-polylysine, sterilization method and application |
| CN116076509A (en) * | 2023-03-14 | 2023-05-09 | 天津科技大学 | Biological agent for preventing and treating gray mold of tomatoes and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897343A (en) * | 2010-08-27 | 2010-12-01 | 周立群 | Novel composite plant growth regulator |
| CN102578208A (en) * | 2012-02-27 | 2012-07-18 | 上海海洋大学 | Meat product biological fresh-keeping agent and preparation method thereof |
| WO2013133705A1 (en) * | 2012-03-05 | 2013-09-12 | Ceradis B.V. | Polyelectrolyte complexes for biocide enhancement |
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN104621680A (en) * | 2015-01-14 | 2015-05-20 | 齐鲁工业大学 | Natural composite preservative for rice and preservation method thereof |
-
2016
- 2016-02-22 CN CN201610096437.9A patent/CN105532663A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897343A (en) * | 2010-08-27 | 2010-12-01 | 周立群 | Novel composite plant growth regulator |
| CN102578208A (en) * | 2012-02-27 | 2012-07-18 | 上海海洋大学 | Meat product biological fresh-keeping agent and preparation method thereof |
| WO2013133705A1 (en) * | 2012-03-05 | 2013-09-12 | Ceradis B.V. | Polyelectrolyte complexes for biocide enhancement |
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN104621680A (en) * | 2015-01-14 | 2015-05-20 | 齐鲁工业大学 | Natural composite preservative for rice and preservation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| GU LI-QIANG: "Tomato Resistance to Botrytis cinerea Induced by Chitosan", 《AGRICULTURAL SCIENCE & TECHNOLOGY》 * |
| 莫树平 等: "ε-聚赖氨酸复合防腐剂对常见致病菌和污染菌的抑制作用", 《食品与机械》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730081A (en) * | 2019-02-26 | 2019-05-10 | 南京轩凯生物科技有限公司 | A kind of agriculture bactericide composition |
| CN111480498A (en) * | 2020-04-07 | 2020-08-04 | 浙江大学 | Method for preventing fruit gray mold by low-sulfur treatment before picking |
| CN113234766A (en) * | 2021-06-03 | 2021-08-10 | 淮北师范大学 | Biosynthesis method of epsilon-polylysine by adding microbial elicitor |
| CN113234766B (en) * | 2021-06-03 | 2022-04-05 | 淮北师范大学 | Biosynthesis method of epsilon-polylysine by adding microbial elicitor |
| CN113785842A (en) * | 2021-10-20 | 2021-12-14 | 海南大学 | Pesticide composition for preventing and treating plant fungal diseases and preparation method thereof |
| CN113875779A (en) * | 2021-10-20 | 2022-01-04 | 海南大学 | Bactericide composition and preparation method thereof |
| CN113875779B (en) * | 2021-10-20 | 2022-10-28 | 海南大学 | Bactericide composition and preparation method thereof |
| CN114732018A (en) * | 2022-03-22 | 2022-07-12 | 西北农林科技大学 | Biological agent, application and tomato gray mold prevention and treatment method |
| CN114938809A (en) * | 2022-04-29 | 2022-08-26 | 中国农业科学院植物保护研究所 | Compound composition of wuyiencin and epsilon-polylysine, sterilization method and application |
| CN116076509A (en) * | 2023-03-14 | 2023-05-09 | 天津科技大学 | Biological agent for preventing and treating gray mold of tomatoes and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105532663A (en) | Biological agent for controlling gray molds by being sprayed on flowers | |
| CA2926731C (en) | Strigolactone formulations and uses thereof | |
| JP2018525445A (en) | Non-toxic plant pharmaceutical composition and method and use thereof | |
| CN102164493A (en) | Foliarly applicable silicon nutrition compositions & methods | |
| CN104738094B (en) | A kind of plant immune lures anti-protein composition and its preparation method and application | |
| KR100518776B1 (en) | Microbial agent for the prevention of disease of ginseng containing Bacillus subtilis strain | |
| CN100486434C (en) | Application of burdock fructooligosaccharides as disease-resistant elicitors in agriculture | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| CN107257625A (en) | Active agent combinations | |
| CN106998688A (en) | Active agent combinations | |
| CN104904761A (en) | Biopesticide and usage method thereof | |
| CN103039468A (en) | Oligochitosan-propineb composition used for plant protection | |
| KR20190031929A (en) | Composition for controlling pine wilt disease comprising acibenzolar-S-methyl as effective component and method for controlling pine wilt disease using the same | |
| KR101917368B1 (en) | Selective medium for isolation of Botyrtis cinerea and Use Thereof | |
| CN106922694A (en) | A kind of bactericidal composition | |
| CN101946802A (en) | Bactericide composition with synergistic effect | |
| KR101232936B1 (en) | Simultaneous Controlling Agent for Plant Diseases Comprising Strawberry Pests Comprising Botanical Extract and Controlling Method Using The Same | |
| CN107455377B (en) | Pesticide aqueous solution for promoting plant growth and prolonging effective period of deciduous fruit trees for clearing garden and preparation method thereof | |
| CN102273454A (en) | Application of epsilon-poly-l-lysine to control of mould disease of crops | |
| DeGenring et al. | Inhibition of Botrytis cinerea growth and suppression of gray mold on petunia leaves using chitosan | |
| CN101258850B (en) | Application of 2-cyano-3-amino-3-phenylacrylate for preventing and controlling corps disease | |
| Mehta et al. | Chitosan and chitosan-based nanoparticles in horticulture: past, present and future prospects | |
| CN103181387B (en) | Compound bactericide and application thereof | |
| CN104920384A (en) | Sterilizing composite comprising benzothiostrobin and chlorothalonil and application of sterilizing composite | |
| CN104041496A (en) | Compound bactericide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |