CN103181387B - Compound bactericide and application thereof - Google Patents
Compound bactericide and application thereof Download PDFInfo
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- CN103181387B CN103181387B CN201310099685.5A CN201310099685A CN103181387B CN 103181387 B CN103181387 B CN 103181387B CN 201310099685 A CN201310099685 A CN 201310099685A CN 103181387 B CN103181387 B CN 103181387B
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- fludioxonil
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Abstract
The invention discloses a compound bactericide and an application thereof. The compound bactericide is prepared from mandipropamid and fludioxonil, and the mass ratio of mandipropamid to fludioxonil is (1-9): (1-90), preferably (1-5):(1-5), and more preferably (1-3):1. The compound bactericide is applicable to prevention of vegetable downy mildew, late blight, botrytis, cucumber root rot, pepper phytophthora blight and other plant diseases. The fast-acting property and the persistence of the compound bactericide are good, the mechanism of action is unique, the acting sites are more, the bacteriocidal spectrum is wide, the biosecurity is high, the preparation process is simple, the cost is low, and the economic benefit is remarkable.
Description
Technical field
The invention belongs to pesticide field, be specifically related to a kind of composite bactericide and application thereof.
Background technology
Using chemical agent is the most effective means of controlling plant diseases, but the applicable single bactericide of long-term high dose continuously, easily causes the problems such as the remaining of medicament, environmental pollution and drug resistant fungal development.Composite or the mixture of rational chemical bactericide has expansion fungicidal spectrum, improves control efficiency, extends dispenser interval, reduces dosage, reduces poisoning, reduces and remain, delay disease fungus drug resistance and the feature such as drug-fast generation and development.Bactericide compounded is one of effective ways solved the problem.
Mandipropamid is carboxylic acid amide series bactericidal agent, and this series bactericidal agent is a kind of medicament of novel structure, by suppressing the formation of the cumulative interference cell wall of germ phosphide, thus plays the effect of killing germ
[1-3].Carboxylic acid amide series bactericidal agent also comprises dimethomorph, flumorph and benzene metsulfovax etc., normal with the pharmacy mix such as mancozeb and tmtd, prevent and treat the oomycetes diseases such as potato late blight (Phytophthora infestans), downy mildew of cucurbits (Pseudoperonospora cubensis) and capsicum epidemic disease (Phytophthora capsici) and improvement to the resistance of phenyl amide series bactericidal agent in play an important role.The commercial product of mandipropamid is 250g/L mandipropamid SC, and it all has good preventive effect to large absolutely number by ovum microbial leaf portion and fruit disease.To the spore being in the sprouting stage, there is higher activity, and mycelia can be suppressed to grow up and sporulation.It can be rapidly absorbed by blade, and rests in leaf table wax coat, shields to blade.This product obtained the whole world in 2006 in Austria and Korea S and ratifies first, and in listing in 2007.This product obtains registration in November, 2007 in China, and there are Germany, Belgium, Holland etc. in the country having obtained registration at present.Colombia, new two orchids, France, Italy and bar two are also by this product of introducing.This product is low toxicity to fish, bird, honeybee, silkworm.250g/L mandipropamid SC registers for preventing and treating capsicum epidemic disease, peronophythora litchi, potato late blight and two melon epidemic diseases in China
[4-7].
Fludioxonil is a kind of pyroles bactericide of novel structure, mainly through suppressing the phosphorylation of MAPK/histidine kinase in disease fungus infiltration intracellular signaling, many alcohols such as the glycerine in thalline are caused to increase, thus suppress conidia germination, germ tube to extend and mycelial growth, finally cause germ dead
[8].Pyroles bactericide is the novel non-internal-absorting wide-spectrum bactericide of a class of the nitre pyroles antibiotic derivative exploitation produced by pseudomonas
[9,10], mainly comprise fludioxonil, fenpiclonil and fluorine acid imide three kinds, this series bactericidal agent, due to characteristics such as efficient, safety, low consumptions, is chosen as by EPA the product that is free from risk
[11].It is reported, fludioxonil is to penicillium expansum (Penicillium expansum), Botrytis cinerea (Botrytis cinerea)
[12], rhizoctonia (Rhizoctonia spp.)
[13], rod method (Alternaria spp.)
[14]good inhibitory action is all had Deng the mycelial growth of pathogen and spore germination.Fludioxonil is in China mainly through seed treatment or soil treatment controlling plant diseases, but fludioxonil is developed as wetting powder by Syngenta Co., Ltd over the past two years, is used for spraying control tomato or ornamental plant gray mold, and shows excellent control efficiency
[15].
Cucumber root rot is often caused by multiple pathogen Combined Infection, as fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and Phytophthora Root Rot (Phytophthora drechsleri) etc., all use 30% to dislike mould Lingshui Spring agent 800 times of liquid, 3% and dislike mould first frost aqua 650 times of liquid irrigating roots.In addition, fusarium root rot and Phomopsis root rot also available 12.5% carbendazim soluble powder 250 times of liquid, 60% good fortune. first sulphur wetting powder 700 times of liquid, 50% chlorobromide isocyanuric acid soluble powder, 1000 times of liquid irrigating roots.Rotten mould root rot and phytophthora root rot also available 25% metalaxyl wetting powder 600 times of liquid, 50% fluorine mofetil aluminium wetting powder, 600 times of liquid etc. fill with root.Every strain consumption about 200 milliliters when filling with root, fills with if desired one twice again.Above-mentioned effect of chemical control is often very poor, urgently develops new and effective medicament in production, improves its control efficiency to cucumber root rot.
So far there is not yet the report utilizing mandipropamid and the control of fludioxonil development mixture cucumber downy mildew, potato/tomato late blight, eliminating vegetable botrytis and the disease such as cucumber root rot and capsicum epidemic disease.
Bibliography:
[1]FRAC.CAA working group reports[EB/OL].(2007).Http://www.frac.info.
[2]Kuhn P J，Pitt D，Lee S A，et al.Effects of dimethomorph on themorphology and ultrastructure of Phytophthora[J].Mycol Res，1991，95：333-340.
[3]Erwin D C，Ribeiro O K.Phytophthora diseases worldwide[M].St Paul，MN，USA：American Phytopathological Society Press，1996，55.
[4] the yellow youth, Ye Zhongyin. the characteristic of dimethomorph (DMM) and mechanism of action [J] thereof. pesticide science and management, 2000,21 (5): 27-31.
[5] Chi Huiwei, Diao Jie, Nie Kaisheng, etc. new type bactericide mandipropamid [J]. agricultural chemicals, 2007,46 (1): 52-54.
[6] Wang Li, Zhu Jiehua, Wang Wenqiao, etc. novel germicide Phytophthora infestans Toxicity Determination [A]. grain security and plant protection scientific and technical innovation [C]. Scientia Agricultura Sinica technology publishing house, 2009,774-777.
[7] Han Xiuying, Li Hongmin, Ma Zhiqiang, etc. novel germicide AF-12946 is to the virulence of potato late blight and control effects [A]. and the chemical control of Chinese Plants disease is studied [C]. Scientia Agricultura Sinica technology publishing house, 2008,6:73-76.
[8]Hagiwara D，Asano Y，Marui J，et al.Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress[J].Fungal Genetics and Biology，2009，46(11)：868-878.
[9]Gehmann K，Nyfeler，R，Leadbeater，A J，et al.A new phenylpyrrole fungicide for broad-spectrum disease control[A].Brighton Crop Prot.Conf.on Pests and Dis[C].UK.1990：369-376.
[10]Errampalli，D.Effect of fludioxonil on germination and growth of Penicillium expansum and decay in apple cvs.Empire Gala[J].Crop Protection，2004，23：811-817.
[11] Yan Xiaomin, Ning Binke, Wang Lieping, etc. new type bactericide fluorine fludioxonil and New Progresses of R & D [J] thereof. world pesticide, 2010,12 (3): 32-38.
[12]Leroux，P.Recent developments in the mode of action of fungicides[J].Pesticide Management Science，1996，47(2)：191-197.
[13]Iacomi-Vasilescu B，Avenot H，Bataiué-Simoneau N，et al.In vitro fungicide sensitivity of Alternaria species pathogenic to crucifers and identification of Alternaria brassicicola field isolates highly resistant to both dicarboximides and phenylpyrroles[J].Crop Protection，2004，23：481-488.
[14]Hamada M S，Yin Y N，Ma Z H.Sensitivity to iprodione，difenoconazole and fludioxonil of Rhizoctonia cerealis isolates collected from wheat in China[J].Crop Protection，2011，30：1028-1033.
[15] Ji Junjian, Wang Wenqiao, Zhang little Feng, etc. dispenser and indoor fungicide tame are on the impact [J] of tomato Botrytis cinerea on fludioxonil susceptibility continuously. Pesticide Science journal, 2012,14 (1): 35-41.
Summary of the invention
For the weak point that existing composite bactericide exists, the invention provides a kind of composite bactericide preventing and treating vegetable downy mildew, late blight, gray mold and the disease such as cucumber root rot and capsicum epidemic disease of novel, efficient, safety, applied range.
Composite bactericide provided by the present invention is made up of mandipropamid and fludioxonil, and its mass ratio is 1 ~ 9: 1 ~ 90.
Preferably, the mass ratio of mandipropamid and fludioxonil is 1 ~ 5: 1 ~ 5.
More preferably, the mass ratio of mandipropamid and fludioxonil is 1 ~ 3: 1.
Most preferably, the mass ratio of mandipropamid and fludioxonil is 2: 1.
Present invention also offers a kind of compound sterilizing agent formulation.
Compound sterilizing agent formulation provided by the present invention, is made up of auxiliary agent acceptable on above-mentioned composite bactericide and pesticidal preparations.Wherein, described composite bactericide can account for 30% of the described composite bactericide quality of the pharmaceutical preparations.
Described compound sterilizing agent formulation can be the pesticidal preparations of any one formulation, one or more formulations in preferred following formulation: soluble powder (SP), soluble granula (SG), water dispersible granules (WG), aqua (AS), wetting powder (WP), soluble liquid (SL), missible oil (EC), aqueous emulsion (AE), microemulsion (ME) and suspending agent (SC).
Above-mentioned formulation all can adopt method well-known in the art to prepare, normally by active substance and auxiliary agent, filler, abundant mixed dissolution or grinding or granulation, make preparation to use, the auxiliary agent used in fungicide preparation of the present invention comprise dispersant, wetting agent, binding agent, thickener, anti-foaming agent, disintegrant, filler etc. and other to be of value to active substance stable and play the material of drug effect in the formulation, be not particularly limited, concrete component and consumption are determined by test.
Described compound sterilizing agent formulation, its composition specifically can comprise the component of following mass parts:
Mandipropamid 1 ~ 9 part
Fludioxonil 1 ~ 90 part
Filler is appropriate
Described compound sterilizing agent formulation also can comprise the component of following mass parts further:
Said components is 0 time different.
When described compound sterilizing agent formulation is wetting powder, its composition comprises the component of following mass parts:
Described wetting powder also can comprise the component of following mass parts further: dispersant 0 ~ 8 part, but does not comprise 0 part.
Its preparation process is as follows: according to said ratio, by various composition through mix and blend, enters one-level and pulverizes (grain fineness reaches 80 orders); After carrying out mix and blend again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.
When described compound sterilizing agent formulation is water dispersible granules, its composition comprises the component of following mass parts:
Described water dispersible granules also can comprise the component of following mass parts further: dispersant 0 ~ 8 part, but does not comprise 0 part.
Its preparation process is: according to said ratio by mandipropamid, fludioxonil, dispersant, wetting agent and filler through mix and blend; air-flow crushing obtains wetting powder; then wetting powder and water (or with binding agent) are added in kneader simultaneously mediate; make plastic material; finally send this material to Squeezinggranulator; carry out granulation, obtain water dispersible granules product by dry, screening.
Described dispersant is one or more the mixture in lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), 3-oleoyl amino-2-methoxy benzenesulfonic acid sodium (LS), sanlose (CMC), alkylphenol polyoxyethylene, ethylene glycol monobutyl ether, polyoxyethylene alkyl ether and neopelex.
Described lignin sulfonic acid sodium salt specifically can be: sodium lignin sulfonate (M-14), desugar sodium lignin sulfonate (M-9), lignosulfite.Described alkylphenol polyoxyethylene specifically can be: octyl phenol polyoxyethylene (10) ether (OP-10), polyoxyethylene nonylphenol ether.
Described wetting agent is at least one in sodium alkyl benzene sulfonate, alkyl benzene calcium sulfonate, Negel, fatty alcohol-polyoxyethylene ether (JFC), alkylphenol-polyethenoxy base formaldehyde condensate sulfonate (SOPA), wgwin D30, soapberry powder and bay alcohol radical sodium sulphate.
Described sodium alkyl benzene sulfonate specifically can be neopelex.Described Negel specifically can be nekal (Nekal BX).
Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite and bentonite.
Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic and polyvinyl alcohol.
Described thickener is one or more in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate and polyvinyl alcohol.
Described anti-foaming agent is silicone anti-foaming agent M10 or anti-foaming agent C.
Described disintegrant is one or more in bentonite, calcium chloride, anhydrous sodium sulfate, kaolin, diatomite, ammonium sulfate, urea, sodium phosphate trimer and sodium pyrophosphate.
Present invention also offers the application of above-mentioned bactericidal composition and compound sterilizing agent formulation.
The application of bactericidal composition provided by the present invention and compound sterilizing agent formulation is its application in controlling plant diseases.Described plant disease refers in particular to the disease of melon and fruit and/or vegetables.
Described plant disease comprises: oomycetes disease, gray mold and root rot; Described oomycetes disease comprises downy mildew, late blight, capsicum epidemic disease etc.Specifically can be cucumber downy mildew, potato late blight, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease and cucumber root rot etc.
The bactericidal composition be made up of mandipropamid and fludioxonil provided by the invention all has good control efficiency to vegetable downy mildew, late blight, gray mold and cucumber root rot and capsicum epidemic disease.The present invention 30% mandipropamid fludioxonil WP 150g a.i./hm
2(1800 times of liquid), 225g a.i./hm
2(1200 times of liquid), 300g a.i./hm
2(900 times of liquid) spraying prevents and treats cucumber downy mildew, potato/tomato late blight, reaches 74.3% ~ 93.1% to the effect of cucumber/graw mold of tomato and capsicum epidemic disease; The effect of filling with root control cucumber root rot reaches 45.3% ~ 67.7%.This composite bactericide quick-acting and lasting effect good, mechanism of action is unique, and action site is many, effectively can delay the drug-fast generation of pathogen and development.This complex preparation preparation technology is simple, and cost is low, remarkable in economical benefits, and diseases prevention spectrum is wide, and biological safety is high.
Embodiment
Below by specific embodiment, the present invention will be described, but the present invention is not limited thereto.
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and biomaterial, if no special instructions, all can obtain from commercial channels.
Medicine in embodiment and experimental example: the former medicine of 96% mandipropamid, the former medicine of 96% fludioxonil, 50% fludioxonil WP, 25% mandipropamid SC are all purchased from first just reaching (China) Investment Co., Ltd; 50% dimethomorph WP, 50% Boscalid WG are all purchased from German BASF AG." % " in following embodiment is mass percentage.
The embodiment of compound sterilizing agent formulation
Embodiment 1: the wetting powder of composite bactericide
Preparation: 30% mandipropamid fludioxonil WP (2: 1, w/w): get the former medicine 20.83kg of 96% mandipropamid, the former medicine 10.42kg of 96% fludioxonil, add 2kgM-9,1.5kgCMC, 5kg neopelex, 60.25kg fine particle calcium carbonate, through mix and blend, enter one-level and pulverize (grain fineness reaches 80 orders); Again after row mix and blend, enter secondary and pulverize (grain fineness reaches 325 orders), be packaged as product.
Embodiment 2-6: wetting powder
Preparation method with embodiment 1, unlike the component that table 1 is listed.
Table 1 embodiment 2-6 component
The quality testing of embodiment 1-6 composite bactericide mandipropamid fludioxonil WP
1) active constituent content: the content utilizing liquid chromatography for measuring mandipropamid and fludioxonil, result shows that the content of active ingredient in embodiment 1-6 mandipropamid fludioxonil WP is all greater than 30.00%.
2) wettability determination: carry out according to GB/T5451-2001 pesticide wettable wettability determination method.The MEBO ribbon gauze of embodiment 1-6 mandipropamid fludioxonil WP is 55s.
3) mensuration of suspensibility: carry out according to GB/T14825-2006 pesticide wettable suspensibility assay method.The suspensibility of embodiment 1-6 mandipropamid fludioxonil WP reaches more than 85%.
4) mensuration of fineness: carry out according to the wet screening in GB/T16150-1995 pesticide wettable fineness assay method.98.5% is reached by the embodiment 1-6 mandipropamid fludioxonil WP of 325 mesh standard sieves (i.e. particle diameter < 44 μm).
5) mensuration of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Moisture≤1.8% in embodiment 1-6 mandipropamid fludioxonil WP.
6) mensuration of mobility: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.Result shows that the flow index of embodiment 1-6 mandipropamid fludioxonil WP is 1.
7) mensuration of foaming characteristic: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The foaming volume of embodiment 1-6 mandipropamid fludioxonil WP is 5mL, meets national standard.
8) mensuration of thermodynamic stability: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.After the storage of embodiment 1-6 mandipropamid fludioxonil WP heat, resolution ratio≤5% of active ingredient, meets national standard.
Embodiment 7: the water dispersible granules of composite bactericide
Preparation: 30% mandipropamid fludioxonil WP (2: 1; w/w): get the former medicine 20.83kg of 96% mandipropamid; the former medicine 10.42kg of 96% fludioxonil; add NNO 2kg; CMC 1.5kg; neopelex 5kg; white carbon 53.07kg; through mix and blend, air-flow crushing obtains can wet-milling, can add in kneader simultaneously mediate by wet-milling with 2kg cyclodextrin and 5kg water; make plastic material; finally send this material to Squeezinggranulator, carry out granulation, obtain water dispersible granules product by dry, screening.
Embodiment 8-12: the water dispersible granules of composite bactericide
Preparation method with embodiment 7, unlike component listed by table 2.
Table 2 embodiment 8-12 component
Embodiment 7-12 composite bactericide mandipropamid fludioxonil WG quality testing
1) active constituent content measuring: the content utilizing liquid chromatography for measuring mandipropamid and fludioxonil.Result shows, in embodiment 7-12, in mandipropamid fludioxonil WG, the content of active ingredient is all greater than 30.00%.
2) determination of moisture: carry out according to azeotropic distillation method in GB/T1600-2001 agricultural chemicals moisture assay method.Result shows, moisture≤1.8% in embodiment 7-12 mandipropamid fludioxonil WG.
3) dispersed mensuration: the dispersiveness adopting long tube test method determination embodiment 7-12 mandipropamid fludioxonil WG, result is the sediments bottom pipe is 0.1mL.
4) wettability determination: adopt measuring graduates test method determination.Result shows, the wetability of embodiment 7-12 mandipropamid fludioxonil WG is good.
5) disintegrative measures: to 100mL tool plug graduated cylinder (the interior high 22.5cm containing 90mL distilled water, internal diameter 28cm) at 25 DEG C, add sample particle (0.5g, 250 ~ 1410 μm), clamp the middle part of graduated cylinder afterwards, clog bung, with the speed of 8r/min around central rotation, until sample complete disintegration in water.Result shows, the disintegration time of embodiment 7-12 mandipropamid fludioxonil WG is 2min.
6) suspension stability: measure according to GB/T14825-2006 method.Result is that the suspensibility of embodiment 7-12 mandipropamid fludioxonil WG reaches more than 90%.
7) mensuration of thermodynamic stability: enclosed by sample in ground wide-mouth bottle, after storing 4 weeks, analyzes embodiment 7-12 mandipropamid fludioxonil WG active constituent content in (50 ± 1) DEG C insulating box.Result shows that active ingredient resolution ratio is less than 5%.
Embodiment 13: the aqueous emulsion of composite bactericide
Preparation: 30% mandipropamid fludioxonil WP (2: 1, w/w): get the former medicine 20.83kg of 96% mandipropamid, the former medicine 10.42kg of 96% fludioxonil, adds xanthans 1.2kg, NNO 0.8kg, CMC 1.5kg, neopelex 5kg, through mix and blend, under high velocity agitation, water 60.25kg is joined in oil phase, forms the aqueous emulsion of favorable dispersibility.Quality testing meets relevant criterion.
Embodiment 14: the suspending agent of composite bactericide
30% mandipropamid fludioxonil WP (2: 1, w/w): get the former medicine 20.83kg of 96% mandipropamid, the former medicine 10.42kg of 96% fludioxonil, adds NNO 2kg, CMC 1.5kg, neopelex 5kg, water 60.25kg take water as medium, active component, auxiliary agent is added in sand milling still to mix with abrasive media and grinds, obtain homogeneous suspension liquid body, liquid is separated obtained suspending agent with abrasive media.Quality testing meets relevant criterion.
The bactericidal effect of compound sterilizing agent formulation
Experimental example 1: different ratio composite bactericide is to the virulence test of cucumber root rot bacterium
1.1 for examination material
1.1.1 for examination bacterial classification: cucumber root rot bacterium, picking up from Gande village of Zhao Cun township two, Dingzhou City in Hebei Province, having fusarium pine root fungus (Fusarium solani), Phomopsis pine root fungus (Phomopsis sp), rotten mould pine root fungus (Pythium myriotylum) and Phytophthora Root Rot (Phytophthora drechsleri) through being separated pathogen identification.
1.1.2 for examination medium: potato dextrose agar (PDA) medium (potato 200g, glucose 20g, agar 20g, deionized water 1000mL).
1.1.3 supply reagent product: the wetting powder of embodiment 1-6, mandipropamid and fludioxonil quality proportioning are 1 ~ 9: 1, prepare the test medicine of mandipropamid and fludioxonil weight proportion 1 ~ 9: 1 ~ 90 equally, carry out virulence test with former medicine.
1.2 test method
1.2.1 colony diameter mensuration bactericide and mixture are to the virulence of cucumber root rot bacterium
By the former medicine of 96% mandipropamid and the former medicinal acetone solution of 96% fludioxonil, be diluted to the liquid of series concentration again, by liquid with dissolve after be cooled to about 60 DEG C PDA medium mix according to volume ratio 1: 9, add the culture dish of diameter 9cm, make pastille PDA culture medium flat plate, blank adds the coordinative solvent of equivalent; Be that the card punch of 5mm is beaten along colony edge and got bacterium cake by the cucumber root rot bacterium diameter of preculture 4d, be inoculated into the PDA culture medium flat plate central authorities containing series concentration, every ware 1 ferfas cake, mycelia faces down, cover ware lid, each process repetition 3 times, is placed in 25 DEG C of incubators and cultivates 4d, adopts right-angled intersection method to measure colony diameter.Calculate the inhibiting rate of each concentration liquid, utilize DPS Software of Data Statistics to obtain virulence regression equation (Y=a+bx), the EC of mandipropamid, fludioxonil and composition thereof suppression cucumber root rot bacterium mycelial growth
50value and correlation coefficient.
Inhibiting rate (%)=(contrast bacterium colony increases diameter-process bacterium colony and increases diameter) × 100/ (contrast bacterium colony increases diameter)
1.2.2 the virulence synergist of fungicide compounding
According to the mixed coefficient of synergism of Wadley (1967) formulae discovery different agents:
SR＝EC
50(th)/EC
50(ob)
In above formula, a, b represent mandipropamid (A), fludioxonil (B) two kinds of bactericide shared ratio in the mixture; EC
50and EC (A)
50(B) the actual observation EC of A and B is represented respectively
50value, EC
50(th) represent that a: b mixed theoretical EC pressed by A, B two kinds of bactericide
50value, EC
50(ob) for a: b mixed actual observation EC pressed by A, B two kinds of bactericide
50value.SR > 1.5, synergy; SR < 0.5, antagonism; SR represents addition between 0.5 and 1.5.
2. result of the test
As known from Table 3, mandipropamid and fludioxonil mixture suppress the synergy proportioning of cucumber root rot bacterium mycelial growth coefficient of synergism in 3 ~ 1: 1 scope to be greater than 1.5, have good synergistic effect, when wherein mandipropamid and fludioxonil mass ratio are 2: 1, synergy is the strongest.
Table 3 mandipropamid and fludioxonil mixture are to the virulence synergist of cucumber root rot bacterium
Experimental example 2: embodiment 1 composite bactericide wetting powder (WP) is to the control efficiency of cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato, cucumber root rot and capsicum epidemic disease
1. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the cucumber downy mildew test of pesticide effectiveness
In medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ", " bactericidal agent for preventing and treating cucumber downy mildew test of pesticide effectiveness criterion " carries out.Susceptible variety is cucumber variety " capital is green ".Experimental field is positioned at Gande village of Dingzhou City Zhao Cun township two of Hebei province, breed selection, field planting on January 25 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 25% mandipropamid SC, dispenser dosage is 150g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area is about 18m
2.
Before downy mildew natural occurrence, start to spray medicine share medicine 3 times, interval is 7 days.Spraying time is respectively on March 5th, 2012, March 12, March 19.Jacto-HD400 type manual sprayer is adopted evenly to spray medicine.Be 900L/hm by amount of liquid medicine
2.
Investigation morbidity in 7 days (on March 26th, 2012) after last 1 dispenser.Random 4 sampling surveys in every community, often some investigation 2 strains, whole blade is investigated in every strain, and the percentage accounting for whole blade area by lesion area on each blade divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole leaf area; 3 grades: scab accounts for 6% ~ 10% of whole leaf area; 5 grades: scab accounts for 11% ~ 25% of whole leaf area; 7 grades: scab accounts for 26% ~ 50% of whole leaf area; 9 grades: scab accounts for more than 51% of whole leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 4,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (80.2% ~ 88.8%) to cucumber downy mildew.Spray 30% mandipropamid fludioxonil WP 300g a.i./hm
2preventive effect be 88.8%, be significantly higher than and spray 25% mandipropamid SC 150g a.i./hm
2control efficiency, with 50% dimethomorph WP 375g a.i./hm
2control efficiency suitable.Spray 30% mandipropamid fludioxonil WP 225ga.i./hm
2preventive effect be 84.4%, and spray 25% mandipropamid SC 150g a.i./hm
2preventive effect suitable.Show to adopt 30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm before cucumber downy mildew occurs or when just occurring
2foliage-spray has good control efficiency to cucumber downy mildew.
Table 430% mandipropamid fludioxonil WP is to the control efficiency of cucumber downy mildew
| Process | Sick leaf rate (%) | Disease index | Control efficiency (%) |
| 30% mandipropamid fludioxonil WP 150g a.i./hm 2 | 26.45 | 5.20 | 80.2c |
| 30% mandipropamid fludioxonil WP 225g a.i./hm 2 | 23.40 | 4.09 | 84.4b |
| 30% mandipropamid fludioxonil WP 300g a.i./hm 2 | 22.01 | 2.93 | 88.8a |
| 25% mandipropamid SC 150g a.i./hm 2 | 24.18 | 4.67 | 82.2bc |
| 50% dimethomorph WP 375g a.i./hm 2 | 22.85 | 3.32 | 87.3a |
| Blank | 65.46 | 26.27 | - |
Note: with indifference between lowercase alphabet registration certificate identical after column data significantly (P=0.05).Lower same.
2. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats potato late blight test
Test according to " bactericidal agent for preventing and treating potato late blight test of pesticide effectiveness criterion " in medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Local susceptible variety is Potato Cultivars " Favorita ".Experimental field is positioned at Shizigou township, Chongli County, Hebei province seed farm, and on April 25th, 2012 plants, and line-spacing 0.6m, spacing in the rows 0.3m, potato late blight occurs over the years.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 25% mandipropamid SC, dispenser dosage is 150g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement.Plot area 20m
2if, protection row.
Start to spray medicine before field natural occurrence, share medicine 3 times, 7 ~ 10 days intervals.Spraying time is respectively June 20, June 27, July 4.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, spraying is evenly thoughtful.Be 900L/hm by amount of liquid medicine
2.Whole duration of test affects without harsh weather.
Investigation in 7 days (on July 11st, 2012) incidence after last dispenser.Every community diagonal 5 sampling surveys, often some investigation 2 strains, whole compound leaf is investigated in every strain, and the percentage accounting for whole compound leaf area by lesion area on each compound leaf divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole compound leaf area; 3 grades: scab accounts for 6% ~ 10% of whole compound leaf area; 5 grades: scab accounts for 11% ~ 25% of whole compound leaf area; 7 grades: scab accounts for 26% ~ 50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 5,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (80.5% ~ 88.1%) to potato late blight.Spray 30% mandipropamid fludioxonil WP 300ga.i./hm
2preventive effect be 88.1%, be significantly higher than and spray 25% mandipropamid SC 150g a.i./hm
2control efficiency, with 50% dimethomorph WP 375g a.i./hm
2control efficiency suitable.Spray 30% mandipropamid fludioxonil WP150 ~ 225g a.i./hm
2preventive effect with spray 25% mandipropamid SC 150g a.i./hm
2control efficiency suitable.Show to adopt 30% mandipropamid fludioxonil WP 150 ~ 300ga.i./hm before potato late blight occurs or when just occurring
2carry out foliage-spray, to potato late blight, there is good control efficiency.
Table 530% mandipropamid fludioxonil WP is to the control efficiency of potato late blight
3. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the tomato late blight test of pesticide effectiveness
Experimental field is positioned at Xin Xing village of Dingzhou City Yang Jiazhuan township of Hebei province, is breed selection tomato, and tomato variety is susceptible variety " Borrow's tower ".On February 18th, 2012 plants, line-spacing 0.8m, spacing in the rows 0.3m.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 25% mandipropamid SC, dispenser dosage is 150g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on April 18th, 2012, April 25, May 2.Jacto-HD400 type manual sprayer is adopted evenly to spray medicine.Be 900L/hm by amount of liquid medicine
2.
Investigation in 7 days (on May 9th, 2012) incidence after last 1 dispenser.Random 5 sampling surveys in every community, often some investigation 2 strains, whole compound leaf is investigated in every strain, and the percentage accounting for whole compound leaf area by lesion area on each compound leaf divides disease level.0 grade: without scab; 1 grade: scab accounts for less than 5% of whole compound leaf area; 3 grades: scab accounts for 6% ~ 10% of whole compound leaf area; 5 grades: scab accounts for 11% ~ 25% of whole compound leaf area; 7 grades: scab accounts for 26% ~ 50% of whole compound leaf area; 9 grades: scab accounts for more than 51% of whole compound leaf area.
Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) × 100
Disease index=∑ (the sick number of sheets × relative disease value of series at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 6,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (80.8% ~ 89.4%) to tomato late blight.Spray 30% mandipropamid fludioxonil WP 300g a.i./hm
2preventive effect be 89.4%, be significantly higher than and spray 25% mandipropamid SC 150g a.i./hm
2control efficiency, with 50% dimethomorph WP 375g a.i./hm
2control efficiency suitable.Spray 30% mandipropamid fludioxonil WP 150 ~ 225g a.i./hm
2preventive effect with spray 25% mandipropamid SC 150g a.i./hm
2control efficiency suitable.Show to adopt 30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm before tomato late blight occurs or when just occurring
2carry out foliage-spray, to tomato late blight, there is good control efficiency.
Table 630% mandipropamid fludioxonil WP is to the control efficiency of tomato late blight
4. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the capsicum epidemic disease test of pesticide effectiveness
In medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ", " bactericidal agent for preventing and treating capsicum epidemic disease test of pesticide effectiveness criterion " carries out.Susceptible variety is capsicum variety " the ears of an ox or cow king ".Experimental field is positioned at the solid cities and towns Bei Zhuantoucun in Dingxing County of Hebei province, is breed selection capsicum, line-spacing 0.8m, spacing in the rows 0.3m.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 25% mandipropamid SC, dispenser dosage is 150g a.i./hm
2; 50% dimethomorph WP, dispenser dosage is 375g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 24m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on May 4th, 2012, May 11, May 18.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on May 25th, 2012) is investigated.Random 5 sampling surveys in every community, often some investigation 10 strains.Stage division: 0 grade: anosis; 1 grade: rhizome portion is without scab, and indivedual branch or fruit are fallen ill; 3 grades: rhizome portion slightly fall ill the following branch of half or fruit morbidity; 5 grades: rhizome portion moderate morbidity branch more than half or fruit morbidity; 7 grades: seriously fall ill in rhizome portion, the large portion of plant is wilting; 9 grades: the whole wilting death of plant.Fragmentary morbidity before first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index and preventive effect.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Diseased plant rate (%)=(diseased plant number/investigate total strain number) × 100
Disease index=∑ (diseased plant number × relative disease value of series at different levels)/(investigating total strain number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 7,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (84.7% ~ 93.1%) to capsicum epidemic disease.Spray 30% mandipropamid fludioxonil WP 300g a.i./hm
2preventive effect be 93.1%, be significantly higher than and spray 25% mandipropamid SC 150g a.i./hm
2with 50% dimethomorph WP375g a.i./hm
2control efficiency.Spray 30% mandipropamid fludioxonil WP 225g a.i./hm
2preventive effect with spray 25% mandipropamid SC 150g a.i./hm
2with 50% dimethomorph WP 375g a.i./hm
2control efficiency suitable.Show to adopt 30% mandipropamid fludioxonil WP 150 ~ 300ga.i./hm before capsicum epidemic disease occurs or when just occurring
2carry out foliage-spray, to capsicum epidemic disease, there is good control efficiency.
Table 730% mandipropamid fludioxonil WP is to the control efficiency of capsicum epidemic disease
| Process | Diseased plant rate (%) | Disease index | Control efficiency (%) |
| 30% mandipropamid fludioxonil WP 150g a.i./hm 2 | 17.00 | 2.44 | 84.7c |
| 30% mandipropamid fludioxonil WP 225g a.i./hm 2 | 10.00 | 1.67 | 89.6b |
| 30% mandipropamid fludioxonil WP 300g a.i./hm 2 | 6.00 | 1.11 | 93.1a |
| 25% mandipropamid SC 150g a.i./hm 2 | 8.00 | 1.67 | 89.6b |
| 50% dimethomorph WP 375g a.i./hm 2 | 9.50 | 1.72 | 89.2b |
| Blank | 30.50 | 15.94 | - |
5. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the gray mold of cucumber test of pesticide effectiveness
Test according to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " GB/T17980.28-2000 " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ".Cucumber variety is conventional variety " De Ruite 787 ".Experimental field is positioned at Gande village of Dingzhou City Zhao Cun township two of Hebei province, field planting on February 14 in 2012.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 50% Boscalid WG, dispenser dosage is 300g a.i./hm
2; 50% fludioxonil WP, dispenser dosage is 100g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on March 6th, 2012, March 13, March 20.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on March 27th, 2012) is investigated.Investigation incidence, every community gets 5 points at random, whole fruits of often some investigation 2 strains, the sick fruit number that record gray mold causes.Gray mold of cucumber is investigated in units of fruit: 0 grade: without scab; 1 grade: residual flower morbidity; 3 grades: fall ill in areola portion; 5 grades: scab length accounts for less than 10% of fruit; 7 grades: scab length accounts for 11% ~ 25% of fruit; 9 grades: scab length accounts for more than 26% of fruit.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Diseased fruit rate (%)=(sick fruit number/investigation total fruit number) × 100
Disease index=∑ (sick fruit number × relative disease value of series at different levels)/(investigation total fruit number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 8,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (76.0% ~ 92.5%) to gray mold of cucumber.Spray 30% mandipropamid fludioxonil WP 300g a.i./hm
2preventive effect be 92.5%, suitable with the control efficiency spraying 50% Boscalid WG 300g a.i./hm2 and 50% fludioxonil WP 100ga.i./hm2, be significantly higher than the control efficiency spraying 30% mandipropamid fludioxonil WP 150 ~ 225g a.i./hm2.30% mandipropamid fludioxonil WP150 ~ 300g a.i./hm is adopted when showing that front or just generation occur gray mold of cucumber
2carry out foliage-spray, to gray mold of cucumber, there is good control efficiency.
Table 830% mandipropamid fludioxonil WP is to the control efficiency of gray mold of cucumber
| Process | Diseased fruit rate (%) | Disease index | Control efficiency (%) |
| 30% mandipropamid fludioxonil WP 150g a.i./hm 2 | 8.16 | 1.87 | 76.0c |
| 30% mandipropamid fludioxonil WP 225g a.i./hm 2 | 5.56 | 1.28 | 83.4b |
| 30% mandipropamid fludioxonil WP 300g a.i./hm 2 | 3.59 | 0.59 | 92.5a |
| 50% Boscalid WG 300g a.i./hm 2 | 3.02 | 0.48 | 94.1a |
| 50% fludioxonil WP 100g a.i./hm 2 | 3.30 | 0.63 | 92.0a |
| Blank | 16.84 | 7.86 | - |
6. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the graw mold of tomato test of pesticide effectiveness
Test according to medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion " GB/T17980.28-2000 " bactericidal agent for preventing and treating eliminating vegetable botrytis test of pesticide effectiveness criterion ".Tomato variety is conventional variety " east victory hooligan ".Experimental field is positioned at Tian Cunpu village, Gao Lin villages and small towns, Xushui County, Hebei province, field planting on February 10 in 2012.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 150g a.i./hm
2, 225g a.i./hm
2, 300g a.i./hm
2.Contrast medicament: 50% Boscalid WG, dispenser dosage is 300g a.i./hm
2; 50% fludioxonil WP, dispenser dosage is 100g a.i./hm
2, blank spray clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 18m
2.
Premorbid starts to spray medicine, and share medicine 3 times, interval is 7 days.Spraying time is respectively on March 4th, 2012, March 11, March 18.Jacto-HD400 type manual sprayer is adopted evenly thoughtfully to spray medicine.Be 900L/hm by amount of liquid medicine
2.
After last 1 dispenser, 7 days (on March 25th, 2012) is investigated.Investigation incidence, every community gets 5 points at random, whole fruits of often some investigation 2 strains, the sick fruit number that record gray mold causes.Gray mold fruit grade scale is: 0 grade: without scab; 1 grade: residual petal morbidity or column cap morbidity; 3 grades: sepal rots or column cap morbidity spreads to areola portion; 5 grades: areola portion has and infiltrates spot but without mould layer; 7 grades: there is mould layer in areola portion but does not expand to other position; 9 grades: the mould layer in areola portion expands to other position.Calculate diseased fruit rate, disease index and control efficiency according to above stage division, in data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Diseased fruit rate (%)=(sick fruit number/investigation total fruit number) × 100
Disease index=∑ (sick fruit number × relative disease value of series at different levels)/(investigation total fruit number × 9) × 100
Control efficiency (%)=[(check plot disease index-treatment region disease index)/check plot disease index] × 100
As known from Table 9,30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliage-spray has good control efficiency (74.3% ~ 90.3%) to graw mold of tomato.Spray 30% mandipropamid fludioxonil WP 300g a.i./hm
2preventive effect be 90.3%, suitable with the control efficiency spraying 50% Boscalid WG 300g a.i./hm2 and 50% fludioxonil WP 100ga.i./hm2, be significantly higher than and spray 30% mandipropamid fludioxonil WP 150 ~ 225g a.i./hm
2control efficiency.30% mandipropamid fludioxonil WP150 ~ 300g a.i./hm is adopted when showing that front or just generation occur graw mold of tomato
2carry out foliage-spray, to graw mold of tomato, there is good control efficiency.
Table 930% mandipropamid fludioxonil WP is to the control efficiency of graw mold of tomato
| Process | Diseased fruit rate (%) | Disease index | Control efficiency (%) |
| 30% mandipropamid fludioxonil WP 150g a.i./hm 2 | 7.18 | 1.91 | 74.3c |
| 30% mandipropamid fludioxonil WP 225g a.i./hm 2 | 3.82 | 1.09 | 85.7b |
| 30% mandipropamid fludioxonil WP 300g a.i./hm 2 | 3.80 | 0.72 | 90.3a |
| 50% Boscalid WG 300g a.i./hm 2 | 2.69 | 0.53 | 93.1a |
| 50% fludioxonil WP 100g a.i./hm 2 | 3.34 | 0.69 | 90.8a |
| Blank | 14.43 | 7.55 | - |
7. 30% mandipropamid fludioxonil WP in embodiment 1 prevents and treats the cucumber root rot test of pesticide effectiveness
Experimental field is positioned at Gande village of Dingzhou City Zhao Cun township two of Hebei province, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Test medicine: 30% mandipropamid fludioxonil WP dispenser dosage is 1800 times of liquid, 1200 times of liquid, 900 times of liquid.Contrast medicament: 50% Boscalid WG, dispenser dosage is 1500 times of liquid; 50% fludioxonil WP, dispenser dosage is 4500 times of liquid, and blank fills with clear water.Often process and repeat for 4 times, Gong24Ge community, randomized arrangement, plot area 24m
2.
Before cucumber root rot occurs, every strain perfusion liquid 250mL.After medicine, 15d investigates each process root rot a situation arises, random 5 sampling surveys in every community, often some investigation 10 strains.Record morbidity strain number, calculates control efficiency according to diseased plant rate.In data acquisition SPSS13.0 software, Fisher ' s least significant difference (LSD) measures and carries out statistical analysis.
Diseased plant rate (%)=(diseased plant number/investigate total strain number) × 100
Control efficiency (%)=[(check plot diseased plant number-treatment region diseased plant number)/check plot diseased plant number] × 100
As known from Table 10,30% mandipropamid fludioxonil WP, 1800 ~ 900 times of liquid irrigating roots have good control efficiency (45.3% ~ 67.5%) to cucumber root rot, are significantly higher than the control efficiency of 25% mandipropamid SC, 1500 times of liquid and 50% fludioxonil WP, 4500 times of liquid.Show that premorbid adopts 30% mandipropamid fludioxonil WP, 1800 ~ 900 times of liquid irrigating roots to have good control efficiency to cucumber root rot.
Table 10 30% mandipropamid fludioxonil WP is to the control efficiency of cucumber root rot
| Process | Diseased plant rate (%) | Control efficiency (%) |
| 30% mandipropamid fludioxonil WP, 1800 times of liquid | 15.00 | 45.3c |
| 30% mandipropamid fludioxonil WP, 1200 times of liquid | 11.00 | 60.3b |
| 30% mandipropamid fludioxonil WP, 900 times of liquid | 9.00 | 67.5a |
| 25% mandipropamid SC 1500 times liquid | 20.50 | 26.8d |
| 50% fludioxonil WP 4500 times liquid | 19.50 | 30.4d |
| Blank | 28.00 | - |
Field trial shows 30% mandipropamid fludioxonil WP 150 ~ 300g a.i./hm
2foliar spray all has good preventive and therapeutic effect to cucumber downy mildew, potato/tomato late blight, cucumber/graw mold of tomato and capsicum epidemic disease, 30% mandipropamid fludioxonil WP, 900 ~ 1800 times of liquid irrigating roots have good control efficiency to cucumber root rot, can application in field produces.
Experimental example 3: the composite bactericide mandipropamid fludioxonil control cucumber downy mildew in embodiment 7,13,14
Experimental field is positioned at Gande village of Dingzhou City Zhao Cun township two of Hebei province, and cucumber variety is conventional variety " De Ruite 787 ", field planting on February 14 in 2012, line-spacing 1m, spacing in the rows 0.3m.
Dispenser dosage is 150g a.i./hm
2(1800 times of liquid), 225g a.i./hm
2(1200 times of liquid), 300g a.i./hm
2(900 times of liquid).Result shows, the mandipropamid fludioxonil WP 150 ~ 300g a.i./hm in embodiment 7,13,14
2foliage-spray has good control efficiency (81.0% ~ 90.5%) to cucumber downy mildew.
Mandipropamid fludioxonil in table 11 embodiment 7,13,14 is to the control efficiency of cucumber downy mildew
Claims (5)
1. a composite bactericide, is made up of mandipropamid and fludioxonil; Wherein, the mass ratio of described mandipropamid and fludioxonil is 2:1.
2. a compound sterilizing agent formulation, is made up of auxiliary agent acceptable on composite bactericide according to claim 1 and pesticidal preparations.
3. compound sterilizing agent formulation according to claim 2, is characterized in that: the formulation of described compound sterilizing agent formulation is selected from following at least one: wetting powder, water dispersible granules, missible oil, aqueous emulsion, microemulsion and suspending agent.
4. compound sterilizing agent formulation according to claim 3, is characterized in that: described auxiliary agent comprises following at least one: dispersant, wetting agent, binding agent, thickener, anti-foaming agent, disintegrant and filler.
5. composite bactericide according to claim 1 or the application of compound sterilizing agent formulation in controlling plant diseases according to any one of claim 2-4;
Described plant disease is selected from following at least one: cucumber downy mildew, potato late blight, tomato late blight, gray mold of cucumber, graw mold of tomato, capsicum epidemic disease and cucumber root rot.
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| CN1221526C (en) * | 2000-05-17 | 2005-10-05 | 辛根塔参与股份公司 | Novel phenyl-proparylether derivatives |
| CN101421230A (en) * | 2001-11-16 | 2009-04-29 | 辛根塔参与股份公司 | Novel phenyl-propargylether derivatives |
| EP2329713A1 (en) * | 2008-08-18 | 2011-06-08 | Mitsui Chemicals Agro, Inc. | Plant disease control agent comprising d-tagatose as active ingredient, and plant disease control method |
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| CN1221526C (en) * | 2000-05-17 | 2005-10-05 | 辛根塔参与股份公司 | Novel phenyl-proparylether derivatives |
| CN101421230A (en) * | 2001-11-16 | 2009-04-29 | 辛根塔参与股份公司 | Novel phenyl-propargylether derivatives |
| EP2329713A1 (en) * | 2008-08-18 | 2011-06-08 | Mitsui Chemicals Agro, Inc. | Plant disease control agent comprising d-tagatose as active ingredient, and plant disease control method |
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| Title |
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| 13种杀菌剂对生姜腐霉茎基腐病菌的毒力测定;元菊丹 等;《农药》;20110831;第50卷(第8期);第617-619、623页 * |
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