CN100502653C - Compound bactericidal agent for prevention and control of wheat fungi diseases, and its application - Google Patents

Compound bactericidal agent for prevention and control of wheat fungi diseases, and its application Download PDF

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CN100502653C
CN100502653C CNB2006100115918A CN200610011591A CN100502653C CN 100502653 C CN100502653 C CN 100502653C CN B2006100115918 A CNB2006100115918 A CN B2006100115918A CN 200610011591 A CN200610011591 A CN 200610011591A CN 100502653 C CN100502653 C CN 100502653C
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seed
wheat
tmtd
parts
agent
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CN101044852A (en
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王文桥
张小风
韩秀英
马志强
李红霞
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Jiangxi Zhengbang Biological Chemical Co., Ltd.
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Institute of Plant Protection Hebei Academy of Agricultural and Forestry Sciences
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Abstract

A composite bactericide for preventing and eliminating the fungal diseases of wheat is prepared from the active component consisting of triazole-type bactericide and protecting agent, filler, and assistant.

Description

A kind of composite bactericide and application thereof that prevents and treats wheat fungal diseases
Technical field
The present invention relates to pesticide field, relate to a kind of composite bactericide and application thereof that prevents and treats wheat fungal diseases particularly.
Background technology
Wheat is the important cereal crops of China, and banded sclerotial blight, powdery mildew, rust, root rot and full rot are the main fungal diseases that influences China's Wheat Production, are the trend that increases the weight of to take place in recent years, cause the wheat heavy losses.Lack high anti-wheat breed or the forfeiture of wheat breed disease resistance in the production, chemical control is still the main means of these a few class diseases of control and the important leverage of wheat high yield.
Wheat sharp eyespot is a kind of soil-borne disease, all can infect in the past at wheat grain filling, and it infects the position mainly at leaf sheath and stem stalk.The common method of preventing and treating is to adopt dressing seed in the fall sowing time, be the occurrence in peak period of diseases such as wheat sharp eyespot, rust and powdery mildew spring, it is popular to only depend on fungicide-treated seed to be difficult to the control disease, should in time spray medicine control, double wheat powdery mildew and the rust controlled according to PD in wheat shooting stage of turning green.
Banded sclerotial blight had become the common disease of wheat in recent years, renewal along with wheat breed, generally lack anti-banded sclerotial blight kind, the improving of the raising of wheat planting density and water and fertilizer condition (the particularly increase of inorganic nitrogeneous fertilizer consumption), the generation of banded sclerotial blight and harm increase the weight of year by year, in the Yangze river and Huai river basin, middle and lower reaches winter wheat district, the Yellow River extensively takes place, its generation area is moved northward again gradually in recent years, development is very fast in the middle and south, Hebei, can cause mashed bud, dead seedling, the mashed stem of flower stalk, withered booting, hazard symptoms in various degree such as withered dead ears, the time and two to three one-tenth of the fast underproduction are seriously retransmitted in about one one-tenth of the underproduction.Chemical control is the important means of control wheat sharp eyespot harm.
Medicaments such as past carbendazim, thiophanate-methyl, jinggangmeisu are used to prevent and treat wheat sharp eyespot, found afterwards that triazolone, Triadimenol, alkene azoles alcohol, Tebuconazole triazole bactericidal agents such as (Garrick shows) treatment effect of dressing seed was better, found in recent years that 2.5% suspension seed-coating agent when suitable happy was higher to the wheat sharp eyespot field efficacy.
Tmtd is a multidigit point protection bactericide; be widely used in the control of wheat diseases; received the excellent protection effect, and made that as companion's medicine of multiple systemic fungicide drug effect strengthens, consumption reduces, for agricultural production and environmental protection play an important role.
Tmtd be usually used in systemic fungicide be mixed the seed dressing or the spraying crop pest, the mixture preparation of for example existing tmtd and Tebuconazole is declared patent and is registered as product prevents and treats multiple wheat diseases such as banded sclerotial blight, and the mixture of tmtd, Tebuconazole and the no track of gram is registered as product and prevents and treats multiple wheat, corn disease insect pest.
Such as, application number is 00110885, denomination of invention is a kind of tebuconazole containing bactericide composition, relates to the composition pesticide that contains Tebuconazole, two kinds of bactericide of tmtd, or add plant growth regulators in addition, the minimum plant element is made synergist.And the method for using multiple wheat diseases such as this composition for preventing and controlling wheat sharp eyespot.Above-mentioned two kinds of bactericide are used with, or add synergist in addition, and multiple wheat diseases such as wheat sharp eyespot are had synergistic effect.But it is still useless in the mixture of registered Tebuconazole and tmtd in the mixture of control wheat sharp eyespot, only have loose smut of wheat to be used as the mixture of controlling object registration Tebuconazole and tmtd in the wheat fungal diseases, formulation has wetting powder and suspension seed-coating agent.
The triazole type medicament is a big class wide-spectrum bactericide, having in plant upwards conduction, lasting period long, germ virulence height, germ are difficult for developing immunity to drugs and to characteristics such as non-target organism safety.Protective fungicides such as new varieties such as own azoles alcohol and tmtd are used the control efficiency and double wheat powdery mildew and the rust etc. controlled that whether improve wheat sharp eyespot with and are waited research.The alternative medicament of screening mechanism of action difference and high-efficiency low-toxicity and safely and effectively mixture preparation to delay sheath blight fungus the resistance that substitutes medicament produced and the banded sclerotial blight of control anti-benzimidazole type medicament significant.
At present, Shang Weijian is combined into the research report that composite bactericide is used to prevent and treat wheat diseases with the own azoles alcohol of triazole bactericidal agent, Flusilazole, alkene azoles pure and mild nitrile bacterium azoles and tmtd.
Summary of the invention
The composite bactericide that the purpose of this invention is to provide the control wheat fungal diseases of a kind of novel, efficient, safety, applied range.
Another object of the present invention provides a kind of application of composite bactericide in preparation control wheat sharp eyespot, wheat powdery mildew, wheat rust, take-all, root rotof flax and other wheat diseases agricultural chemicals that prevents and treats wheat fungal diseases.
In order to realize the object of the invention, technical scheme of the present invention:
The composite bactericide of control wheat fungal diseases of the present invention, or/and auxiliary agent is formed, wherein, active component is made up of triazole bactericidal agent and protectant bactericide by active component, filler.
The composite bactericide of control wheat fungal diseases of the present invention, triazole bactericidal agent one or more the mixing of selecting oneself in azoles alcohol, Flusilazole, alkene azoles alcohol or the nitrile bacterium azoles in the active component; The protectant bactericide is selected from tpn, mancozeb, Propineb or the tmtd one or more mixing.
The composite bactericide of control wheat fungal diseases of the present invention contain the active component combination of synergistic effect, and ratio is arbitrarily between active component, as long as can produce synergistic effect.Generally speaking, the composition of described active component is counted by ratio of weight and the number of copies: triazole bactericidal agent: protectant bactericide=1～10:10～100.
Preferably, the composition of active component is counted by ratio of weight and the number of copies: triazole bactericidal agent: protectant bactericide=1～10:10～50.
More preferably, the composition of active component is counted by ratio of weight and the number of copies: own azoles alcohol: tmtd=1:5～15.
Composite bactericide of the present invention can be mixed with wetting powder, water dispersible granules, suspension seed-coating agent or do the preparation that hangs formulations such as agent according to technology well known in the art.
When described composite bactericide was made wetting powder, it comprised following component and content:
1～10 part of triazole bactericidal agent
10～50 parts of protective fungicides
1～8 part of wetting agent
1～8 part of dispersant
10～80 parts of fillers.
Its preparation process is: according to said ratio, various compositions are stirred through mixing, enter one-level and pulverize (grain fineness reaches 80 orders); After mixing stirring again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.The wheat growth phase adopts 20%～50% wetting powder to be diluted to cauline leaf spraying, every 666.7m 2(mu) but with 20%～50% wet-milling, 44～220g (active ingredient 22～44g/ mu), add water 60kg and be diluted to suspension (diluting 600～1200 times), prevent and treat banded sclerotial blight, powdery mildew and rust.
When described composite bactericide was made water dispersible granules, it comprised following component and content:
1～10 part of triazole bactericidal agent
10～50 parts of protective fungicides
2～8 parts of wetting agents
2～8 parts of dispersants
10～80 parts of fillers
5～10 parts of water and/or binding agents.
Its preparation process is: according to said ratio triazole bactericidal agent, protectant, dispersing aid, wetting agent and filler are stirred through mixing; comminution by gas stream obtains wetting powder; then wetting powder and water (or having binding agent) are added in the kneader simultaneously and mediate; make plastic material; send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.The wheat growth phase adopts 20%～50% granule to be diluted to cauline leaf spraying, every 666.7m 2(mu) but with 20%～50% wet-milling, 44～220g (active ingredient 22～44g/ mu), add water 60kg and be diluted to suspension (diluting 600～1200 times), prevent and treat banded sclerotial blight, powdery mildew and rust.
When described composite bactericide was made suspension seed-coating agent, it comprised following component and content:
1～10 part of triazole bactericidal agent
10～50 parts of protective fungicides
2～8 parts of wetting agents
2～8 parts of dispersants
2～8 parts of suspension aids or binding agents
0.1～1 part of anti-bubble auxiliary agent
30～60 parts in water.
Its preparation process is: mix after ball mill mixes pulverizes according to said ratio, enter sand mill then and pulverize and promptly obtain finished product.Sowing stage of wheat adopts 20%～50% suspension seed-coating agent to handle seed, every 100kg seed adds water or contains insecticide emulsion 1.5kg wetting, with 20%～50% suspension seed-coating agent, 44～330g (active ingredient 22～66g/ mu), mix thoroughly to sow and prevent and treat banded sclerotial blight, full rot and root rot again.
Make when doing outstanding agent when described composite bactericide, it comprises following component and content:
1～10 part of triazole bactericidal agent
10～50 parts of protective fungicides
2～8 parts of wetting agents
2～8 parts of dispersants
2～8 parts of suspension aids or binding agents
2～8 parts of disintegrants
10～80 parts of fillers.
Its preparation process is: mix after the back disperses according to said ratio, through roller drying, carry out packing after the coarse crushing and get final product.The wheat growth phase adopts 20%～50% dried outstanding dilution agent to become cauline leaf spraying, every 666.7m 2(mu) but with 20%～50% wet-milling, 44～220g (active ingredient 22～44g/ mu), add water 60kg and be diluted to suspension (diluting 600～1200 times), prevent and treat banded sclerotial blight, powdery mildew and rust.It is wetting that perhaps every 100kg seed adds water 5kg, mixes thoroughly with 20%～50% dried outstanding agent 44～330g (active ingredient 22～66g/ mu) and can sow application process for spraying, prevents and treats banded sclerotial blight, full rot and root rot.
In composite bactericide of the present invention, described filler is to be selected from white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite one or more.
The adjuvant used this area usual auxiliaries that is of composite bactericide of the present invention is as wetting agent, dispersant, suspension aids, binding agent (thickener), anti-bubble auxiliary agent or disintegrant etc.
Specifically, dispersant: one or more in the mixture of lignin sulfonic acid sodium salt (M-9), naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), 4-oleoyl-methoxy benzenesulfonic acid sodium salt (LS), sanlose (CMC), lignosulfite, alkylphenol polyoxyethylene (OP-10), ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex.
Wetting agent: a kind of in pelopon A, fatty alcohol-polyoxyethylene ether (JFC) or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate (SOPA).
Anti-foaming agent: a kind of among silicone anti-foaming agent M10 or the anti-foaming agent C.
Disintegrant: a kind of in bentonite, kaolin, diatomite, urea, sodium phosphate trimer or the sodium pyrophosphate.
Suspension aids: aluminium-magnesium silicate.
Binding agent is one or more in carboxymethyl cellulose, gum Arabic, cyclodextrin or the polyvinyl alcohol.
The purposes of composite bactericide of the present invention in preparation control wheat sharp eyespot, wheat powdery mildew, wheat rust, take-all and other wheat fungal diseases agricultural chemicals.The preferably application in preparation control wheat sharp eyespot agricultural chemicals.
Researcher of the present invention is by the virulence of more different types of bactericide (triazole type medicament, methoxy acrylic medicament and protective fungicide, benzimidazole, jinggangmeisu, tolelofos-methyl etc.) to rhizoctonia cerealis; filter out efficient medicament to wheat fungal diseases; measure efficient medicament and protectant and mix synergy wheat fungal diseases by different proportionings; determine the higher and significant best proportioning of synergistic effect of virulence, the efficient medicament of preventing and treating wheat fungal diseases for development lays the foundation.
The complex preparation synergistic effect of control wheat fungal diseases of the present invention is remarkable, tmtd and own azoles alcohol wetting powder (active constituent content is 20%～50%) the spraying control efficiency that contains of the present invention reaches 66.0%～83.8%, described tmtd and own azoles alcohol suspension seed-coating agent (active constituent content is 20%～50%) the seed dressing control efficiency that contains reaches 63.3%～80.5%, apparently higher than unitary agent, but be better than conventional medicine 50% carbendazim wet-milling, but 50% tmtd wet-milling, 6% Garrick show suspension seed-coating agent, but the mixture preparation of 5% hexaconazole microemulsion and tmtd and Tebuconazole 6% penta good fortune wet-millings.This complex preparation quick-acting and lasting effect are good, and mechanism of action and site are many, can effectively delay drug-fast generation of pathogen and development.Preparation technology is simple for this complex preparation, and cost is low, remarkable in economical benefits, and the diseases prevention spectrum is wide, the biological safety height.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
The preparation of embodiment 1 built wettable powder
44% contains tmtd and own azoles alcohol wetting powder (1:10): get 95% own azoles alcohol 4.22kg, 95% tmtd 42.11kg adds 2kg M-9,1.5kgCMC, 5kg neopelex, 45.17kg fine particle calcium carbonate, stir through mixing, enter one-level and pulverize (grain fineness reaches 80 orders); After row mixes stirring again, enter secondary and pulverize (grain fineness reaches 325 orders), be packaged as product.
Embodiment 2-6
The preparation method is with embodiment 1, and different is following component:
Figure C200610011591D00081
Embodiment 7-11
The preparation method is with embodiment 1, and different is following component:
*The addition of active ingredient is the amount of pure active ingredient.
The preparation of embodiment 12 composite water dispersible granules
44% tmtd diniconazole water dispersible granule (1:10): get 95% own azoles alcohol 4.22kg, 95% tmtd 42.11kg adds NNO 2kg; CMC1.5kg; neopelex 5kg, white carbon 45.17kg stirs through mixing; but comminution by gas stream obtains wet-milling; but wet-milling added simultaneously with 2kg cyclodextrin and 5kg water mediate in the kneader, make plastic material, send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.
Embodiment 13-17
The preparation method is with embodiment 12, and different is following component:
Figure C200610011591D00092
Embodiment 18-22
The preparation method is with embodiment 12, and different is following component:
Figure C200610011591D00093
Figure C200610011591D00101
*The addition of active ingredient is the amount of pure active ingredient
The preparation of embodiment 23 composite suspension seed-coating agents
The own azoles alcohol of 44% tmtd suspension seed-coating agent (1:10): get 4.22 parts of 95% own azoles alcohol, 42.11 parts of 95% tmtd, add 10 parts in polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex mixture (1:2:2), SOPA4 part, 3 parts in aluminium-magnesium silicate powder, 0.5 part of silicone anti-foaming agent M10,36.17 parts in water.Each composition mixed after ball mill mix pulverize after, enter sand mill and pulverize, promptly obtain finished product.
Embodiment 24-28
The preparation method is with embodiment 23, and different is following component:
Figure C200610011591D00102
Embodiment 29-33
The preparation method is with embodiment 23, and different is following component:
Figure C200610011591D00111
The embodiment 34 composite preparations of doing outstanding agent
The own azoles alcohol of 44% tmtd is done outstanding agent (1:10): get 4.22 parts of 95% own azoles alcohol, 42.11 parts of 95% tmtd add OP-105 part, 10 parts of lignosulfites (in solids), 5 parts in aluminium-magnesium silicate powder, 33.67 parts of white carbons.With former medicine, paper pulp concentrate and auxiliary agent mix disperse after, behind roller drying, carry out packing after the coarse crushing and get final product.
Embodiment 35-39
The preparation method is with embodiment 34, and different is following component:
Figure C200610011591D00112
Embodiment 40-44
The preparation method is with embodiment 34, and different is following component:
Figure C200610011591D00121
Experimental example 1: different medicaments are to the control efficiency test of rhizoctonia cerealis
This experiment purpose is to study the control efficiency of different medicaments to rhizoctonia cerealis.
1.1, for the examination material
1.1.1, provide by plant protection institute of Shandong Agricultural University for examination bacterial classification rhizoctonia cerealis (Rhizoctonia cerealis).
1.1.2 wheat breed " 4185 " for the high sense of examination wheat breed banded sclerotial blight.
1.1.3 for the reagent product
The former medicine of 95% own azoles alcohol, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
5% hexaconazole microemulsion, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
The former medicine of 95% Tebuconazole, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
12.5% tebuconazole aqueous emulsion, Kesheng Group Co., Ltd., Jiangsu Prov.;
The former medicine of 95.27% alkene azoles alcohol, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
The former medicine of 95% triazolone, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
But 25% triazolone wet-milling, Bayer crop science company;
The former medicine of 95% Triadimenol, Jiangsu Yancheng Lvye Chemical Co., Ltd.;
The former medicine of 96.6% penconazole industry, HangZhou YuLong Chemical Engineering Co., Ltd;
The former medicine of 96% tmtd, Tianjin Agricultural Chemical Inst.;
50% thiram wettable powder, Hebei are preced with imperial agricultural chemicals Co., Ltd;
95% propiconazole, Jiangsu Qizhou Green Chemical Co., Ltd;
25% propiconazole emulsifiable concentrate, Jiangsu Qizhou Green Chemical Co., Ltd;
The former medicine of 90% Flusilazole, Tianjin be day chemical industry Co., Ltd for a long time;
40% Flusilazole missible oil, Jiangsu Jiannong Agrochemical Co., Ltd.;
The former medicine of 85% Propineb, Jiangsu Province LiMin chemical engineering Co.,Ltd;
The former medicine of 95% tolelofos-methyl, chemical plant, the East Sea, Lianyungang, Jiangsu Province;
20% tolelofos-methyl missible oil, the highly dense Kang Fengnongization in Shandong Co., Ltd;
The former medicine of 95% pcnb industry, Linfen, Shanxi Province Organic Chemical Plant;
90% dislikes the industrial former medicine of mould spirit, Shandong Jingbo Agrochemicals Ltd.;
The former medicine of 98% carbendazim industry, Shandong Huayang Science ﹠. Technology Co., Ltd.;
50% carbendazol wettable powder, Jiangyin, Jiangsu insecticide factory;
The former medicine of 93.6% thiophanate-methyl industry, Shandong Huayang Science ﹠. Technology Co., Ltd.;
The former medicine of 60% jinggangmeisu industry, Qianjiang Biochemistry Co., Ltd., Zhejiang Prov.;
5% jinggangmeisu soluble powder, Qianjiang Biochemistry Co., Ltd., Zhejiang Prov.;
2.5% suitable suspension seed-coating agent when happy just reaches earlier (China) Investment Co., Ltd;
The former medicine of 95% Difenoconazole industry, Jiangsu Gengyun Chemical Co., Ltd.;
10% generation high-moisture shot agent (Difenoconazole) just reaches earlier (China) Investment Co., Ltd;
96% tpn, Jiangsu LiMin chemical engineering Co.,Ltd;
75% Da Kening (tpn) suspending agent just reaches earlier (China) Investment Co., Ltd;
The former medicine of 88.2% Fluoxastrobin, sea, Hunan sharp chemical industry group company;
25% Amici reaches (Fluoxastrobin) wetting powder, just reaches earlier (China) Investment Co., Ltd;
The former medicine of 93.2% SYP-Zo48 (SYP-Z048), Shenyang Chemical Engineering Inst;
The former medicine of 99% carboxin, Xi'an literary composition chemical industry Co., Ltd far away;
40% defends the good fortune suspension seed-coating agent, the U.S. favourable incoming road chemical company;
The former medicine of 95% nitrile bacterium azoles industry, Jiangsu Gengyun Chemical Co., Ltd.;
The former medicine of 95% Prochloraz industry, the special farmingization of Hubei bass Co., Ltd;
25% Prochloraz missible oil, the special farmingization of Hubei bass Co., Ltd;
80% mancozeb, chemical plant, Guangzong County, Xingtai;
10.2% good fortune amylalcohol suspension seed-coating agent, Chongqing kind clothing agent factory;
6% penta good fortune wetting powders, pesticide chemical Co., Ltd of Jilin Province Jiization group
1.2 test method
1.2.1 bactericide and composition thereof is to the virulence of rhizoctonia cerealis under isolated condition
Adopt radially growth method of mycelia.Kind of bactericide original drug surplus own azoles alcohol, the Tebuconazole etc. 20 is dissolved with suitable solvent (as acetone, carrene, chloroform etc.), be diluted to the soup of series concentration again, add the culture dish of diameter 9cm, make pastille PDA flat board, blank adds the coordinative solvent of equivalent; Mancozeb, Propineb are directly added in the PDA medium that dissolves by fine ground back, make the pastille medium.The bacterium cake of laying diameter 0.3cm at the rhizoctonia cerealis colony edge that grows on the PDA culture medium flat plate with card punch, be seeded in the dull and stereotyped central authorities of pastille medium and blank, every ware 1 ferfas cake, mycelia faces down, cover the ware lid, place under the incubator dark condition and measure colony diameter behind the cultivation 7d.Calculate the inhibiting rate of each drug concentration, carry out the probit value of inhibiting rate and the linear regression analysis between the concentration logarithm value again, obtain the regression equation that every kind of medicament suppresses mycelial growth, obtain in view of the above and suppress mycelial growth 50% (EC 50Be worth) and 90% (EC 90Value) valid density.
1.2.2 bactericide and mixture are to the preventive effect of rhizoctonia cerealis under condition of living body
1.2.2.1 spray-on process
Under aseptic technique, with cultivating at diameter is that rhizoctonia cerealis on the PDA flat board of 9cm changes in the PD medium in the 300mL triangular flask (150mL/ bottle), 1 of every bottle graft PDA flat board that carries disease germs, seal to seal film, place shaken cultivation case (HZQ-F160, Harbin east connection electronic technology development corporation, Ltd.) in 23.5 ℃, shaken cultivation 10d under the illumination condition, pull the PDA flat board that carries disease germs out, putting into agitator (blendor HR 2860) smashs to pieces, with fine sand, thin loam and fertilizer (Jiamusi Lvzhou ferment Co., Ltd) 1:1:0.3 (v/v) mixture mixing (1 flat board that carries disease germs blends thing+1.25L mixture) are made bacterium soil.With the humus soil 150mL plastic cup of packing into, every glass of 100mL is by real.Wheat seed is soaked 1d in clear water, change water 3 times, pull out, broadcast humus soil surface, broadcast 10 for every glass, lid bacterium soil, every glass of 30mL every glass of compacting.Soup is sprayed onto bacterium soil surface (blank spray clear water, every glass of 2mL), and every concentration repeats 5 times.Changing the plastic cup of handling over to plastic casing (preserves moisture among 70cm * 45cm * 18cm), cultivates 20d under the room temperature, the investigation state of an illness.
State of an illness grade scale is as follows: 0 grade: anosis; 1 grade: the leaf sheath morbidity, cane does not have scab; 3 grades: the leaf sheath morbidity, scab is around cane＜1/2; 5 grades: scab is around cane 1/2～3/4; 7 grades: scab is around cane〉3/4; 9 grades: complete stool is withered.Calculate disease index and relative control effect according to following formula: disease index=[∑ (diseased plant numbers at different levels * corresponding sick progression)/investigation total strain number * the highest morbidity progression] * 100; Relative control effect=[(contrast disease index-processing disease index)/contrast disease index] * 100%.Carry out the probit value of relative control effect and the linear regression analysis between the concentration logarithm value again, obtain the virulence regression equation that every kind of medicament and composition thereof suppresses rhizoctonia cerealis, obtain in view of the above on the live body and the rhizoctonia cerealis relative control effect to be reached 50% and 90% valid density EC 50Value and EC 90Value.
1.2.2.2 seed soaks the medicine method
At the dull and stereotyped central authorities of the PDA of diameter 9cm inoculation rhizoctonia cerealis, every ware connects the bacterium cake of a diameter 0.3cm, covers the ware lid, places to be cultured to mycelia under 23.5 ℃, dark condition and to extend to plate edge.Load weighted reagent agent is added deionized water, be diluted to the suspension of series concentration, wheat seed is immersed soup, every concentration liquid soaks 200 seeds (blank soaks with clear water), pulling seed behind the 1h out dries in the air on qualitative filter paper, naturally after drying, broadcast and covering with on the PDA flat board of rhizoctonia cerealis, every concentration is established 5 wares, every ware is broadcast 40 seeds, cover ware lid, place 23.5 ℃, illumination condition to cultivate 1d down, every ware dress fine sand is full to ware.Cultivate 15d under 16h illumination/d, every 2d waters and preserves moisture for 1 time, promotes the wheat morbidity.According to the 1.2.2.1 investigation state of an illness, calculate every processing wheat sharp eyespot disease index and relative control effect.Carry out the probit value of relative control effect and the linear regression analysis between the concentration logarithm value again, obtain the virulence regression equation that every kind of medicament and composition thereof suppresses rhizoctonia cerealis, obtain in view of the above on the live body and the rhizoctonia cerealis relative control effect to be reached 50% and 90% valid density EC 50Value and EC 90Value.
1.2.3 fungicide compounding synergistic effect
Calculate the not mixed coefficient of synergism of with medicament according to Wadley (1967) formula:
EC 90 ( th ) = a + b a / EC 90 ( A ) + b / EC 90 ( B )
SR＝EC 90(th)/EC 90(ob)
A, b represent own azoles alcohol (A), two kinds of bactericide of tmtd (B) shared ratio in mixture in the following formula; EC 90(A) and EC 90(B) represent the actual observation EC of A and B respectively 90Value, EC 90(th) two kinds of bactericide of expression A, B are pressed the mixed theoretical EC of a:b 90Value, EC 90(ob) be that two kinds of bactericide of A, B are pressed the mixed actual observation EC of a:b 90Value.SR〉1.5, synergy; SR＜0.5, antagonism; SR represents summation action between 0.5 and 1.5.
2. result and analysis
As known from Table 1,9 kinds of sterols biosynthesis inhibitors (SBI ' s) show different inhibition activity to the rhizoctonia cerealis mycelial growth, the EC of the pure and mild Tebuconazole of wherein own azoles 50Value is respectively 0.023 μ g/mL and 0.024 μ g/mL, apparently higher than the inhibition activity of other non-SBI ' s medicaments such as other triazole type medicaments such as Triadimenol, triazolone, propiconazole, Flusilazole, penconazole, Difenoconazole and Prochloraz.The therefore selected pure and mild Tebuconazole of own azoles is made further toxicity test.
Table 1 bactericide is to the inhibitory action of rhizoctonia cerealis mycelial growth
Figure C200610011591D00151
Table 2 method for soaking seed is measured the preventive effect of bactericide to wheat sharp eyespot
Figure C200610011591D00152
As known from Table 2, adopt the soup of the preparation preparation of the pure and mild Tebuconazole of own azoles to soak that wheat seed infects seed to the prevention rhizoctonia cerealis and Fructus Hordei Germinatus has effect preferably, be better than preparations such as tmtd, carbendazim, Wei Fu, tolelofos-methyl, the preventive effect that reaches (Fluoxastrobin) with the Amici is approaching.
With medicine liquid spray to the soil surface that carries rhizoctonia cerealis, the pure and mild Tebuconazole of own azoles has higher preventive effect to wheat sharp eyespot, is better than conventional dose (table 3) such as similar medicaments such as Prochloraz, enemy's power take off, Flusilazole, generation height (Difenoconazole) and carbendazim, thiophanate methyl, tolelofos-methyl, jinggangmeisu.
Table 3 spray-on process is measured the control efficiency of bactericide to wheat sharp eyespot
Figure C200610011591D00161
Comprehensive isolated condition reaches the own azoles alcohol measurement result higher to the rhizoctonia cerealis virulence under the condition of living body down; and it is single higher with pesticide resistance problem and cost occurring according to own azoles alcohol; so own azoles alcohol is mixed with lower-cost protective fungicide tmtd, screens best proportioning.
Table 4 own azoles alcohol under stripped and condition of living body is mixed to the synergistic effect of rhizoctonia cerealis with tmtd
Figure C200610011591D00162
As known from Table 4, own azoles alcohol and the tmtd synergy ratio range that suppresses the rhizoctonia cerealis mycelial growth that is mixed is 1:4～14 or 1:18～30, and wherein the be mixed synergistic effect that suppresses the rhizoctonia cerealis mycelial growth of 1:10 is the strongest.In addition, adopt method for soaking seed and spray-on process to measure under condition of living body and show that the synergy ratio range is 1:4～18, show that all the synergistic effect after own azoles alcohol is mixed by 1:10 with tmtd is the most remarkable, this ratio is decided to be best proportioning.
Experimental example 2: compound drug preparation
After definite 1:10 is own azoles alcohol and the best proportioning of tmtd, carry out preparation according to embodiment 1,12,23,34.
The own azoles alcohol of 44% tmtd wetting powder (own azoles alcohol: tmtd=1:10): the former powder of the pure and mild tmtd of own azoles that takes by weighing q.s, adding suspension aids, wetting aid, dispersing aid and filler stirs through mixing, enter one-level and pulverize (grain fineness reaches 80 orders), after row mixes stirring again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.Application process is prevented and treated 600～1200 times of banded sclerotial blight amounts of application for spraying.
44% tmtd diniconazole water dispersible granule (own azoles alcohol: tmtd=1:10); take by weighing the former powder of the pure and mild tmtd of own azoles of q.s; stir through mixing after adding suspension aids, wetting aid, dispersing aid and filler; comminution by gas stream obtains wetting powder; then wetting powder and gauge water, binding agent are added in the kneader and mediate, make plastic material, send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.Application process is prevented and treated 600～1200 times of banded sclerotial blight amounts of application for spraying.
(own azoles alcohol: tmtd=1:10), take by weighing the former powder of the pure and mild tmtd of own azoles of q.s adds auxiliary agents such as wetting agent, dispersant, thickener, defoamer to the own azoles alcohol of 44% tmtd suspension seed-coating agent.Each composition is mixed after ball mill mixes pulverizing, enter the sand mill pulverizing then and promptly obtain finished product.Application process is prevented and treated banded sclerotial blight amount of application 22～66ga.i./100kg seed for seed dressing.
The dried outstanding agent of the own azoles alcohol of 44% tmtd (own azoles alcohol: tmtd=1:10), the former powder of the pure and mild tmtd of own azoles that takes by weighing q.s adds dispersant, wetting agent, suspension aids or thickener, disintegrant, after mix disperseing,, carry out packing after the coarse crushing and get final product through roller drying.Application process is prevented and treated 600～1200 times of banded sclerotial blight amounts of application for spraying.
The own azoles alcohol of 44% tmtd granule (own azoles alcohol: tmtd=1:10); the former powder of the pure and mild tmtd of own azoles that takes by weighing q.s adds dispersant, wetting agent, suspension aids or thickener, disintegrant; after mixing dispersion; through roller drying; carry out after the coarse crushing adding in the kneader and mediate, make plastic material, send this material to Squeezinggranulator with gauge water, binding agent; carry out granulation, obtain the granule product by dry, screening.Application process is prevented and treated 600～1200 times of banded sclerotial blight amounts of application for spraying.
Experimental example 3: the compound drug mode of action is measured
1 different bactericide are to the mode of action test of wheat powdery mildew
The susceptible wheat of plantation in the flowerpot is selected 3 consistent leaf phase wheat seedlings of growth in the greenhouse, test medicine is mixed with suitable concentration tests, and every processing 5 basins repeat 4 times.
1.1 protective effect: the soup that sprays series concentration is in the wheat leaf blade positive and negative; to spray clear water is blank; treat that soup dries; after placing the growth case to cultivate 5,10,15 days; with trembling powder method inoculation wheat leaf blade; place 24 ℃ of illumination conditions of growth case to cultivate 10 days down, when blank blade occurring degree is suitable for adding up, investigate and respectively handle the state of an illness, calculate disease index and control efficiency.
1.2 therapeutic action: plant wheat leaf blade in flowerpot with trembling the powder method, place 24 ℃ of illumination conditions of growth case to cultivate 1,3,5 day down, after treating the field planting of white powder germ, spray the series concentration soup in the blade positive and negative (is blank to spray clear water), place the growth case to continue to be cultured to 6,4,2 days plant.The state of an illness is respectively handled in investigation when blank blade occurring degree is suitable for adding up, and calculates disease index and control efficiency.Wheat powdery mildew grade scale (is unit with the blade): 0 grade: anosis; 1 grade: lesion area accounts for below 5% of whole blade area; 3 grades: lesion area accounts for 6%～15% of whole blade area; 5 grades: lesion area accounts for 16%～25% of whole blade area; 7 grades: lesion area accounts for 26%～50% of whole blade area; 9 grades: lesion area accounts for more than 50% of whole blade area.
The own azoles alcohol of table 5 44% tmtd wetting powder is to the preventive effect of wheat powdery mildew
Figure C200610011591D00181
But the own azoles alcohol of table 6 44% tmtd wet-milling is to the therapeutic action of wheat powdery mildew
Figure C200610011591D00182
But from table 5 and table 6 as can be known 600～1200 times of dilutions sprayings of the own azoles alcohol of 44% tmtd wet-milling handle wheat powdery mildew had prevention and therapeutic action preferably, lasting period can reach 15 days, but but but but prevention effect and therapeutic action are significantly higher than contrast medicament 6% penta 600 times of good fortune wet-millings, 600 times of 5% hexaconazole microemulsions, 600 times of 50% tmtd wet-millings, 1000 times of 50% carbendazim wet-millings, 1000 times of 25% triazolone wet-millings.
But the effect of the main disease of the own azoles alcohol of experimental example 4:44% tmtd own azoles alcohol wet-milling and 44% tmtd suspension seed-coating agent control wheat
But the own azoles alcohol of 1.44% tmtd wet-milling control wheat sharp eyespot (Rhizoctonia cerealis) test
Medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion " " bactericidal agent for preventing and treating wheat sharp eyespot test of pesticide effectiveness criterion " carries out.Wheat breed is local susceptible variety " Handan 9587 ".The experimental field is positioned at south, Xu Cun township, south, Guanyao County, Hebei province Xu Cun, sowing on October 3rd, 2004, spacing in the rows 20cm, line-spacing 50cm.Wheat sharp eyespot takes place over the years.Experimental field water and fertilizer condition is better, and mu is executed the chicken manure 3m that becomes thoroughly decomposed before the plantation 3, diammonium phosphate 20kg.The cultivation management term harmonization, the Pass Test requirement.Test medicine: but the own azoles alcohol of 44% tmtd wet-milling, and dispenser dosage is 1200 times of (750g/hm 2), 900 times of (1000g/hm 2), 600 times of (1500g/hm 2).The contrast medicament: but 6% penta 600 times of (1500g/hm of good fortune wet-milling 2); 750 times of (1200g/hm of 5% hexaconazole microemulsion 2); But 1200 times of (750g/hm of 50% carbendazim wet-milling 2); But 50% tmtd wet-milling, dispenser dosage are 600 times of (1500g/hm 2).Randomized arrangement is adopted in the experimental plot.If 3 processing of test medicine, 4 processing of contrast medicament, 1 processing of blank, 4 repetitions, totally 32 sub-districts.Sub-district area 20m 2, establish guard row.Turn green to the shooting stage at wheat, banded sclerotial blight sporadicly takes place and begins medication when laterally spreading, and administration time is on March 15th, 2004 and March 30, shared medicine 2 times, and be 15 days interval.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, reach evenly thoughtful during spraying.With amount of liquid medicine is 900L/hm 2No harsh weather influence during the whole test, temperature 12-24.5C, in early April, 2005 and difference rainfall in the first tenday period of a month in May 1 time, rainfall is respectively 23mm and 29mm, and environmental condition is suitable for the generation of wheat sharp eyespot.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (March 17) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps banded sclerotial blight and takes place.Before the sowing not with medicament handle seed, duration of test does not use other bactericide to carry out disease control, but uses 6000 times of (125g/hm of 50% Aphox wetting powder on March 20th, 2005 2) spray the control wheat aphid, spray 5000 times of (150g/hm of tribenuron-methyl 2) prevent and kill off (the spraying liquid amount 750L/hm of weeds in the ground 2).Wheat sharp eyespot mainly takes place on leaf sheath and the stem stalk in the experimental field.5 samplings of every sub-district diagonal, every some investigation 100 strains, the investigation incidence of disease and withered dead ears rate.Stage division: 0 grade: do not fall ill; 1 grade: leaf sheath is fallen ill but the stem stalk is not fallen ill; 3 grades: the leaf sheath morbidity, and invade stem, but stem stalk scab ring stem less than 1/2; 5 grades: stem stalk scab ring stem surpasses 1/2, but non-lodging or fracture; 7 grades: withered, lodging, withered dead ears.It is very light and even to fall ill before the dispenser, and state of an illness radix is designated as zero.15 days (on March 30th, 2005 after the dispenser for the first time, turn green to the shooting stage), 15 days (on April 14th, 2005 after the dispenser for the second time, shooting stage) a situation arises for the investigation wheat sharp eyespot, and withered dead ears show the withered dead ears that disease phase (on May 20th, 2005) investigation banded sclerotial blight causes.Respectively handle the state of an illness according to above stage division record, calculate the incidence of disease, disease index, withered dead ears rate, relative control effect, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Figure C200610011591D00191
Figure C200610011591D00192
Figure C200610011591D00201
But the own azoles alcohol of table 7 44% tmtd wet-milling is to the control efficiency (2004) of wheat sharp eyespot
Figure C200610011591D00202
But the own azoles alcohol of table 8 44% tmtd wet-milling is to the control efficiency (2005) of wheat sharp eyespot
But table 7 and table 8 data show 1200 times of the own azoles alcohol of 44% tmtd wet-millings, 900 times, 15 days is 66.0%～83.8% to the turn green control efficiency of shooting stage banded sclerotial blight of wheat after 600 times of dilution dispensers first, drug effect increases with the raising of concentration, but contrast medicament 6% penta 600 times of preventive effects of good fortune wet-milling are 61.63%～66.9%, but 600 times of preventive effects of 50% tmtd wet-milling are 55.9%～58.1%, the control efficiency that 5% hexaconazole microemulsion is 600 times is respectively 81.8% and 81.7%, but 1200 times of preventive effects of 50% carbendazim wet-milling are 58.3%～64.4%, but the preventive effect of 1000 times of 25% triazolone wet-millings is 66%～68.9%.But but but the preventive effect of 900 times of hence one can see that the own azoles alcohol of 44% tmtd wet-millings and 600 times is significantly higher than the preventive effect of 1000 times of 600 times of 50% tmtd wet-millings, 6% penta 600 times of good fortune wet-millings, 1200 times of 50% carbendazim and 25% triazolones, and the preventive effect of 600 times of the preventive effect of 600 times of the own azoles alcohol of 44% tmtd wetting powders and 5% hexaconazole microemulsions does not have significant difference.But but the preventive effect of 600 times of the preventive effect of 1200 times of the own azoles alcohol of 44% tmtd wet-millings and 6% penta good fortune wet-millings is suitable.Drug effect after the preventive effect ratio dispenser for the first time after the dispenser for the second time slightly descends, but the significant difference analysis result between the drug effect of the preventive effect of 3 concentration of test medicine and all contrast medicaments is with the result of dispenser for the first time.
But 44% tmtd. behind twice of own azoles alcohol wet-milling (600～1200 times) the spray medicine to the preventive effect 53.6%～73.8% of the shooting stage wheat sharp eyespot of turning green.But the preventive effect that contrast medicament 50% tmtd wet-milling is 600 times is 40.3%～42.1%.The preventive effect that 5% hexaconazole microemulsion is 600 times is 71.3%～73.8%, but suitable with the preventive effect of 600 times of the own azoles alcohol of 44% tmtd wet-millings and 900 times.But but but the preventive effect of 600 times of 1200 times of 50% carbendazim wet-millings, 1000 times of 25% triazolone wet-millings and 6% penta good fortune wet-millings is respectively 47.4%～55.6%, 57%～62% and 56.4%～58.6%, but significantly be lower than the preventive effect of 900 times of the own azoles alcohol of 44% tmtd wet-millings and 600 times.In a word, use the own azoles alcohol of 44% tmtd wetting powder and all wheat sharp eyespot is had the better prevention effect for 600～1200 times, obviously reduced the withered dead ears rate of wheat that banded sclerotial blight causes, the suggestion using dosage is 600～1200 times, but in order to reach the better prevention effect, should use 600～900 times in the heavier field of the state of an illness, to shooting stage and wheat sharp eyespot generation, begin dispenser, spraying volume 900L/hm at the wheat period of seedling establishment 2, and according to weather condition and disease development decision medication interval phase, be 10～15 days general interval.In addition, suggestion is seed dressing prior to seeding, reduces bacterium source and the worm source just sent out, but is combined in the shooting stage of the turning green own azoles alcohol of the 44% tmtd wet-milling of spraying, and reaches higher control efficiency.The above concentration of test medicine has no adverse effects to crop and other beneficial organism.
The own azoles alcohol of 244% tmtd suspension seed-coating agent seed dressing control wheat sharp eyespot
Wheat breed is local susceptible variety " Handan 9587 ", thousand kernel weight 41g.The experimental field is positioned at south, Xu Cun township, south, Guanyao County, Hebei province Xu Cun, is field production winter wheat, sowing on October 3rd, 2004, and spacing in the rows 20cm, line-spacing 50cm, seeding quantity is a 5kg/ mu.Wheat sharp eyespot takes place over the years.Experimental field water and fertilizer condition is better, executes the chicken manure 3m/ mu that becomes thoroughly decomposed before the plantation, diammonium phosphate 20kg/ mu, cultivation management term harmonization, Pass Test requirement.
Test medicine: the own azoles of 44% tmtd alcohol suspension seed-coating agent, seed dressing dosage are 22,33,44,66ga.i./100kg seed.Contrast medicament: 6% Garrick show suspension seed-coating agent, 3g a.i./100kg seed; But 50% tmtd wet-milling, 250ga.i./100kg seed; 5% hexaconazole suspending agent, 3g a.i./100kg seed; 10.2% good fortune amylalcohol suspension seed-coating agent, 76.5ga.i./100kg seed.If 4 processing of test medicine, contrast medicament 4 processing, 1 processing of blank repeats for 4 times, totally 36 sub-districts, sub-district area 20m 2, establish guard row.Randomized arrangement is adopted in the experimental plot.On October 3rd, 2004, before wheat cultivation, dress seed with test medicine and contrast medicament respectively.Earlier weigh required kind of clothing agent, in the ratio of 10kg seed water 150ml, measure required water again, will plant the clothing agent and pour into slowly in the water, after stirring, carry out the wheat seeds dressing again, fully stir, make every seed all be stained with medicament equably by recommended dose.The seed of mixing is placed on can be sowed after dry at shady and cool place.With the seed of mixing clear water is blank.Fine during sowing.Adopt glassware to pack the wheat seed that medicament is mixed, adopt sower evenly to sow, the chemicals treatment district is sowed in sowing blank district more earlier, and by the order sowing of low concentration to high concentration, to avoid owing to using the mechanical planting machine to cause error.After planting the wheat seedling phase of surviving the winter fall in slight snow 2 times, temperature-7-24.5C, wheat (in the early April, 2005 and the first tenday period of a month in May) rainfall 2 times of turning green the shooting stage in spring, rainfall is respectively 23mm and 29mm, temperature 12-25C, environmental condition is suitable for the generation of wheat sharp eyespot.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher, no weeds in the ground.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (on March 20th, 2005) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps banded sclerotial blight and takes place.Duration of test does not use other bactericide to carry out disease control, uses 6000 times of (125g/hm of 50% Aphox suspension seed-coating agent on March 23rd, 2005 2) spray the control wheat aphid, spray 5000 times of (150g/hm of tribenuron-methyl 2) prevent and kill off (the spraying liquid amount 750L/hm of weeds in the ground 2).When half (about 1 week) emerged in the blank district, investigate the situation of emerging of all sub-districts.Investigate the situation of emerging of each sub-district during 3 weeks for the second time, 5 samplings of every sub-district diagonal, every wheat seeding number of 5 :s 500 wheats (100) in the ranks of investigating sowing in every sub-district calculates emergence rate.Wheat sharp eyespot mainly takes place also can cause the withered dead ears of wheat on leaf sheath and the stem stalk in the experimental field.5 samplings of every sub-district diagonal, every some investigation 100 strains, investigation diseased plant rate and withered dead ears rate.But stage division is seen the own azoles alcohol of 144% tmtd wet-milling control wheat sharp eyespot.After planting, blank emerge half and the situation of emerging of observing pelleted seed neat seedling stage have or not poisoning to produce, record poisoning type and degree.After examination wheat maturation, gather in the crops the wheat of each sub-district, weigh, survey and produce.It is very light and even to fall ill before the dispenser, and state of an illness radix is designated as zero.Wheat is turned green, and a situation arises to shooting stage (on April 2nd, 2005) investigation wheat sharp eyespot, and withered dead ears show the withered dead ears that disease phase (on May 20th, 2005) investigation banded sclerotial blight causes.Respectively handle the state of an illness according to above stage division record, calculate the incidence of disease, disease index, withered dead ears rate, relative control effect, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Figure C200610011591D00222
Figure C200610011591D00223
The influence that the seed dressing of the own azoles alcohol of table 9 44% tmtd suspension seed-coating agent is emerged to wheat
Figure C200610011591D00224
As known from Table 9, the emergence rate of four dosage of the own azoles of wheat seed 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) after seed dressing one week of sowing is respectively 50.1%, 48.9%, 46.6%, 45.7%, and the wheat seed emergence rate of contrast medicament 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) seed dressing is 49.4%.Sowing emergence rate after two weeks, to be respectively 90.5%, 90.4%, 90.3%, 90.0%, 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) emergence rate be 90.3%.Without one week of wheat (blank) planting seed of dressing seed and the emergence rate after two weeks being respectively 50.1% and 90.6%.Show that sowing the emergence rate in a week through the own azoles of 44% tmtd alcohol suspension seed-coating agent than the wheat seed of low dosage (22ga.i./100kg seed) seed dressing does not have significant difference with wheat emergence rate without chemicals treatment, three higher dosage of the own azoles of 44% tmtd alcohol suspension seed-coating agent (33,44,66ga.i./100kg seed) seed dressing and 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) seed dressing reduce the wheat emergence rate in one week of sowing, but to sowing emerging not have obviously and influence after two weeks.After sowing for two weeks, the emergence rate of the wheat seed of dressing seed and not dressing seed does not have significant difference.The own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed), 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and slight postponement of 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) seed dressing are emerged.The own azoles of 44% tmtd alcohol suspension seed-coating agent seed dressing (22,33,44,66ga.i./100kg seed) is respectively 63.3%, 71.3%, 77.2%, 80.5% to the preventive effect of wheat sharp eyespot in tillering stage then, drug effect increases with the raising of concentration, but is significantly higher than the preventive effect (48.5%) of 50% tmtd wet-milling (250ga.i./100kg seed).The preventive effect of 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and 5% hexaconazole microemulsion (3ga.i./100kg seed) is respectively 71.4% and 70.8%, with the preventive effect there was no significant difference of the own azoles of 44% tmtd alcohol suspension seed-coating agent 33,44ga.i./100kg seed, but significantly be lower than the preventive effect of test medicine 66ga.i./100kg seed.The preventive effect of 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) is 63.3%, with the preventive effect there was no significant difference of the own azoles of 44% tmtd alcohol suspension seed-coating agent 22ga.i./100kg seed, but significantly be lower than the preventive effect of test medicine 33,44,66ga.i./100kg seed.The own azoles of 44% tmtd alcohol suspension seed-coating agent seed dressing (22,33,44ga.i./100kg seed) drops to 47.2%, 55.9%, 62.5%, 68.4% respectively to the preventive effect of shooting stage next year wheat sharp eyespot, is significantly higher than the preventive effect (40.1%) of tmtd.Reduce to 55.3% and 53.2% respectively under the preventive effect of 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion, with two of the own azoles of 44% tmtd alcohol suspension seed-coating agent preventive effect there was no significant differences, but significantly be lower than the preventive effect of the own azoles alcohol of 44% tmtd suspension seed-coating agent higher dosage (44,66ga.i./100kg seed) than low dosage (22,33ga.i./100kg seed).The preventive effect of 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) is 48.6%, suitable with the preventive effect of the own azoles of 44% tmtd alcohol suspension seed-coating agent 22,33ga.i./100kg seed, but significantly be lower than the preventive effect of test medicine 44,66ga.i./100kg seed.
The own azoles alcohol of table 10 44% tmtd suspension seed-coating agent is to the control efficiency of wheat sharp eyespot
Figure C200610011591D00231
Figure C200610011591D00241
The own azoles of 44% tmtd alcohol suspension seed-coating agent seed dressing (22,33,44,66ga.i./100kg seed) descends more obvious to the preventive effect that withered dead ears show disease phase wheat sharp eyespot, be respectively 38.2%, 44.6%, 53.2%, 57.9%, be significantly higher than the preventive effect that the tmtd companion plants.The preventive effect of 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion reduces to 44.8% and 42.3% respectively, do not have significant difference with two of the own azoles of 44% tmtd alcohol suspension seed-coating agents than the preventive effect of low dosage (22,33ga.i./100kg seed), and significantly be lower than the preventive effect of the own azoles alcohol of 44% tmtd suspension seed-coating agent higher dosage (44,66ga.i./100kg seed).The preventive effect of 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) is 41.6%, suitable with the preventive effect of the own azoles of 44% tmtd alcohol suspension seed-coating agent 22,33ga.i./100kg seed, but significantly be lower than the preventive effect of test medicine 44,66ga.i./100kg seed.
The seed dressing of the own azoles alcohol of table 11 44% tmtd suspension seed-coating agent is to the influence of wheat yield
Figure C200610011591D00242
The wheat yield of the own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) seed dressing is 441.9,458.6,471.9,479.2kg/hm 2, be significantly higher than the wheat yield that not medication seed dressing and tmtd are dressed seed.The output of 6% Garrick show suspension seed-coating agent and the seed dressing of 5% hexaconazole microemulsion is respectively 459.4kg/hm 2And 459.2kg/hm 2, suitable with two output of the own azoles alcohol of 44% tmtd suspension seed-coating agent than the low dosage seed dressing, but significantly be lower than the output that two higher dosage of 44% tmtd own azoles alcohol suspension seed-coating agent are dressed seed.Above-mentioned four dosage seed dressing of the own azoles alcohol of 44% tmtd suspension seed-coating agent is respectively 10.4%, 13.7%, 16.1%, 17.4% to the wheat increase yield rate, is significantly higher than the rate of growth of tmtd seed dressing.The rate of growth of 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed) seed dressing is 11.1%, suitable with the rate of growth (11.6%) of the own azoles alcohol suspension seed-coating agent lowest dose level of 44% tmtd (22ga.i./100kg seed) seed dressing, but significantly be lower than the preventive effect that other three dosage of this test medicine are dressed seed.The rate of growth of 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion (3ga.i./100kg seed) seed dressing is 13.8% and 16.0%, significantly be lower than the rate of growth of the own azoles of 44% tmtd alcohol suspension seed-coating agent higher dosage (44,66ga.i./100kg seed), do not have significant difference with the rate of growth of another dosage (33ga.i./100kg seed) seed dressing.
In a word, the own azoles of 44% tmtd alcohol suspension seed-coating agent with 22,33,44, the dosage seed dressing of 66ga.i./100kg seed has the better prevention effect to wheat sharp eyespot, especially to tillering stage, wheat sharp eyespot had good preventive effect then, wheat sharp eyespot to shooting stage next year also has certain preventive and therapeutic effect, but preventive effect reduces.Seed dressing also reduces the withered dead ears rate of wheat that banded sclerotial blight causes, effect of increasing production is obvious, can large tracts of land promote the use of, the suggestion using dosage is 22,33,44,66ga.i./100kg seed, except adopting the test medicine seed dressing before sowing, also should adopt the big liquid measure spraying of test medicine 1-2 time, reach better control efficiency in wheat shooting stage of turning green.The above concentration of test medicine has no adverse effects to crop and other beneficial organism.The seed dressing of the own azoles alcohol of 3.44% tmtd suspension seed-coating agent prevents and treats root rotof flax (Bipolaris sorokininan) test of pesticide effectiveness
Medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion " " bactericidal agent for preventing and treating cereal class seed-borne disease " carries out.Select wheat susceptible variety " Ji wheat No. 6 " for use, field trial is arranged in Hebei Vocational Technology Normal College agronomy experiment station, Changli County, Hebei province of morbidity over the years, the cultivation condition uniformity of all experimental plots.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher, no weeds in the ground.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (on March 20th, 2005) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps root rot and takes place.Duration of test does not use other bactericide diseases prevention.Test medicine: the own azoles of 44% tmtd alcohol suspension seed-coating agent, seed dressing dosage are 22,33,44,66ga.i./100kg seed.The contrast medicament: 6% Garrick show suspension seed-coating agent, seed dressing dosage is 3g a.i./100kg seed; 50% thiram wettable powder 250ga.i./100kg seed; 5% hexaconazole suspending agent 3g a.i./100kg seed; 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed).Randomized arrangement is adopted in the experimental plot.If 4 processing of test medicine, contrast medicament 3 processing, 1 processing of blank repeats for 4 times, totally 32 sub-districts, sub-district area 20m 2, establish guard row.Before wheat cultivation (on October 3rd, 2004) carry out dressing seed.Earlier weigh required kind of clothing agent, in the ratio of 10kg seed water 150ml, measure required water again, will plant the clothing agent and pour into slowly in the water, after stirring, carry out the wheat seeds dressing again, fully stir, make every seed all be stained with medicament equably by recommended dose.The seed of mixing is placed on can be sowed after dry at shady and cool place.The own azoles alcohol of 44% tmtd suspension seed-coating agent seed dressing dosage is 50ml/100kg seed, 75ml/100kg seed, 100ml/100kg seed, 150ml/100kg seed, the seed dressing dosage of 6% Garrick show suspension seed-coating agent is the 50ml/100kg seed, the seed dressing amount of 5% hexaconazole suspending agent is the 60ml/100kg seed, and the seed dressing amount of 50% thiram wettable powder is the 500g/100kg seed.With the seed of mixing clear water is blank.Fine during sowing.Adopt glassware to pack the wheat seed that medicament is mixed, adopt sower evenly to sow, the chemicals treatment district is sowed in sowing blank district more earlier, and by the order sowing of low concentration to high concentration, to avoid owing to using sower to cause error.With 150kg/hm 2Seeding quantity and contrast equivalent sowing.It is very light and even to fall ill before the dispenser, and state of an illness radix is designated as zero.A situation arises for wheat heading stage investigation root rotof flax, 5 samplings of every sub-district diagonal, every some investigation 50 strains.Respectively handle the state of an illness with reference to wheat sharp eyespot stage division record, calculate diseased plant rate, disease index, relative control effect, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.After examination wheat maturation, gather in the crops the wheat of each sub-district, weigh, to survey and produce, statistics is protected and is produced effect.Protect and produce effect=100% * (treatment region output-check plot output)/check plot output
After planting, blank emerge half and the situation of emerging of observing pelleted seed neat seedling stage have or not poisoning to produce, record poisoning type and degree.
Figure C200610011591D00251
Figure C200610011591D00252
As known from Table 12,4 dosage (22 of the own azoles alcohol suspension seed-coating agent of 44% tmtd, 33,44,66ga.i./100kg seed) seed dressing has preventive effect (62.1%～75.9%) preferably to becoming strain phase root rotof flax, be significantly higher than the preventive effect (27.6%) of contrast medicament 50% tmtd (250ga.i./100kg seed) seed dressing, wherein 2 than low dosage seed dressing (22,33ga.i./100kg seed) preventive effect of preventive effect and two other contrast medicament 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion seed dressing (3ga.i./100kg seed) is suitable, but 2 higher dosage seed dressings (44,66ga.i./100kg seed) preventive effect is significantly higher than 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed), the preventive effect of 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and the seed dressing of 5% hexaconazole microemulsion.
In addition, the wheat yield of 4 dosage of the own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) seed dressing is 446.1～471.0kg/hm 2Be significantly higher than the output of tmtd seed dressing, wherein 2 suitable than low dosage seed dressing (22,33ga.i./100kg seed) output with the output of 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion seed dressing (3ga.i./100kg seed), but the output of 2 higher dosage seed dressings (44,66ga.i./100kg seed) is significantly higher than 10.2% good fortune. the output that amylalcohol suspension seed-coating agent, 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion are dressed seed.It is 11.2%～17.4% that the guarantor of 4 dosage of the own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) seed dressing produces effect, produces effect apparently higher than the guarantor of contrast medicament tmtd, own azoles alcohol and Garrick show.Do not find 4 dosage of the own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) seed dressing causing wheat poisoning and to the bad reaction of other biological.
The seed dressing of the own azoles alcohol of table 12 44% tmtd suspension seed-coating agent prevents and treats root rotof flax
Figure C200610011591D00261
The seed dressing of the own azoles alcohol of 4.44% tmtd suspension seed-coating agent prevents and treats take-all (Gaeumannomyces graminis) test of pesticide effectiveness
" bactericidal agent for preventing and treating take-all " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion (two) ".Select wheat susceptible variety " Shandong wheat 21 " for use, field trial is arranged in evenly morbidity, pathogenic bacterium colony over the years and is in nature decline plot, academy of agricultural sciences, Yantai City, Shandong in the past, the cultivation condition uniformity of all experimental plots.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher, no weeds in the ground.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (on March 20th, 2005) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps full rot to take place.Duration of test does not use other bactericide to carry out disease control.Test medicine: the own azoles of 44% tmtd alcohol suspension seed-coating agent, seed dressing dosage are 22,33,44,66g a.i./100kg seed.The contrast medicament: 6% Garrick show suspension seed-coating agent, seed dressing dosage is 3g a.i./100kg seed; But 50% tmtd wet-milling 250g a.i./100kg seed; 5% hexaconazole suspending agent 3g a.i./100kg seed; 10.2% good fortune amylalcohol suspension seed-coating agent (76.5g a.i./100kg seed).
Randomized arrangement is adopted in the experimental plot.If 4 processing of test medicine, contrast medicament 4 processing, 1 processing of blank repeats for 4 times, totally 36 sub-districts, sub-district area 20m 2, establish guard row.On October 3rd, 2004, before wheat cultivation, dress seed with test medicine and contrast medicament respectively.Earlier weigh required kind of clothing agent, in the ratio of 10kg seed water 150ml, measure required water again, will plant the clothing agent and pour into slowly in the water, after stirring, carry out the wheat seeds dressing again, fully stir, make every seed all be stained with medicament equably by recommended dose.The seed of mixing is placed on can be sowed after dry at shady and cool place.With the seed of mixing clear water is blank.Fine during sowing.Adopt glassware to pack the wheat seed that medicament is mixed, adopt sower evenly to sow, the chemicals treatment district is sowed in sowing blank district more earlier, and by the order sowing of low concentration to high concentration, to avoid owing to using the mechanical planting machine to cause error.With 150kg/hm 2Seeding quantity and contrast equivalent sowing.It is very light and even to fall ill before the dispenser, and state of an illness radix is designated as zero.In the wheat grain filling later stage, every sub-district 5 samplings at random during the take-all peak of occurrence, every of dead ears of investigation are got 1m duplicate rows (about 20 strains), investigate total spike number and dead ears number.50 strain root samples are got in every of root system investigation, towards root, and by strain investigation root system incidence, the percentage classification of being infected with every strain root.Shooting stage is carried out a root system investigation, gathers in the crops the preceding dead ears rate of investigating every sub-district in 7～10 days.0 grade: anosis; 1 grade: the root system onset area accounts for 1%～5%; 3 grades: the root system onset area accounts for 6%～20%; 5 grades: the root system onset area accounts for 21%～40%; 7 grades: the root system onset area accounts for 41%～60%; 9 grades: the root system onset area accounts for more than 61%.Respectively handle the state of an illness according to above stage division record, calculate dead ears rate, disease index, control efficiency, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
The total spike number of dead ears rate (%)=100 * withered dead ears number/investigate
Figure C200610011591D00271
Figure C200610011591D00272
After planting, blank emerge half and the situation of emerging of observing pelleted seed neat seedling stage have or not poisoning to produce, record poisoning type and degree.
The seed dressing of the own azoles alcohol of table 13 44% tmtd suspension seed-coating agent prevents and treats take-all
Figure C200610011591D00273
Figure C200610011591D00281
As known from Table 13,4 dosage (22 of the own azoles alcohol suspension seed-coating agent of 44% tmtd, 33,44,66ga.i./100kg seed) seed dressing has certain preventive effect (43.3%～60.8%) to the wheat root full rot, be significantly higher than the preventive effect (25.6%) of contrast medicament 50% tmtd (250ga.i./100kg seed) seed dressing, wherein 1 preventive effect than low dosage seed dressing (22ga.i./100kg seed) preventive effect and two other contrast medicament 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion seed dressing (3ga.i./100kg seed) is suitable, there was no significant difference, but 3 higher dosage seed dressings (33,44,66ga.i./100kg seed) preventive effect is significantly higher than the preventive effect of 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and the seed dressing of 5% hexaconazole microemulsion.
4 dosage (22 of the own azoles alcohol suspension seed-coating agent of 44% tmtd, 33,44,66ga.i./100kg seed) seed dressing has certain preventive effect (39.7%～53.1%) to wheat full rot milk ripe stage, be significantly higher than the preventive effect (22.3%) of contrast medicament 50% tmtd (250ga.i./100kg seed) seed dressing, wherein 2 than low dosage seed dressing (22,33ga.i./100kg seed) preventive effect of preventive effect and two other contrast medicament 6% Garrick show suspension seed-coating agent and 5% hexaconazole microemulsion seed dressing (3ga.i./100kg seed) is suitable, there was no significant difference, but 2 higher dosage seed dressings (44,66ga.i./100kg seed) preventive effect is significantly higher than the preventive effect of 6% Garrick show suspension seed-coating agent (3ga.i./100kg seed) and the seed dressing of 5% hexaconazole microemulsion.
Investigation does not find that the serious phytotoxicity reaction of the own azoles alcohol of 44% tmtd suspension seed-coating agent seed dressing causing wheat reaches the ill-effect to other biological, and is safe in utilization.
But the own azoles alcohol of the 5.44% tmtd wet-milling control wheat powdery mildew test of pesticide effectiveness
" bactericidal agent for preventing and treating cereal class powdery mildew " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ".The field production wheat is local susceptible variety " Handan 9587 ".The experimental field is positioned at south, Xu Cun township, south, Guanyao County, Hebei province Xu Cun, sowing on October 3rd, 2004, spacing in the rows 20cm, line-spacing 50cm.Wheat powdery mildew takes place over the years.Experimental field water and fertilizer condition is better, and mu is executed the chicken manure 3m that becomes thoroughly decomposed before the plantation 3, diammonium phosphate 20kg.The cultivation management term harmonization, the Pass Test requirement.Test medicine: but the own azoles alcohol of 44% tmtd wet-milling.Dispenser dosage is 1200 times of (750g/hm 2), 900 times of (1000g/hm 2), 600 times of (1500g/hm 2).The contrast medicament: but 600 times of 6% penta good fortune wet-millings, 750 times of (1200g/hm of 5% hexaconazole microemulsion 2), spraying; But 1200 times of (750g/hm of 50% carbendazim wet-milling 2); But 50% tmtd wet-milling, dispenser dosage are 600 times of (1500g/hm 2); Blank: spraying clear water.Randomized arrangement is adopted in the experimental plot.If 3 processing of test medicine, 4 processing of contrast medicament, 1 processing of blank, 4 repetitions, totally 32 sub-districts.Sub-district area 20m 2, establish guard row.Turn green to the shooting stage at wheat, powdery mildew sporadicly takes place and begins medication when laterally spreading, and the time is on March 15th, 2004 and March 30, shared medicine 2 times, and be 15 days interval.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, reach evenly thoughtful during spraying.With amount of liquid medicine is 900L/hm 2No harsh weather influence during the whole test, temperature 12-24.5C, in early April, 2005 and difference rainfall in the first tenday period of a month in May 1 time, rainfall is respectively 23mm and 29mm, and environmental condition is suitable for the generation of wheat powdery mildew.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (March 17) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps powdery mildew and takes place.Before the sowing not with medicament handle seed, duration of test does not use other bactericide diseases prevention, but uses 6000 times of (125g/hm of 50% Aphox wetting powder on March 20th, 2005 2) spray the control wheat aphid, spray 5000 times of (150g/hm of tribenuron-methyl 2) prevent and kill off (the spraying liquid amount 750L/hm of weeds in the ground 2).Diagonal 5 samplings in every sub-district are looked into 0.25m at every 2Plant, wheat 1～5 leaf of shooting stage investigation base portion of standing up, the boot leaf and following first leaf of boot leaf of the every strain of heading " Invest, Then Investigate ".The stage division of powdery mildew (is unit with the blade): 0 grade: anosis; 1 grade: lesion area accounts for below 5% of whole blade area; 3 grades: lesion area accounts for 6%～15% of whole blade area; 5 grades: lesion area accounts for 16%～25% of whole blade area; 7 grades: lesion area accounts for 26%～50% of whole blade area; 9 grades: lesion area accounts for more than 50% of whole blade area.It is very light and even to fall ill before the dispenser for the first time, and state of an illness radix is designated as zero.For the first time 15 days (on April 14th, 2005, shooting stage) after 15 days (on March 30th, 2005, turning green), the dispenser for the second time after the dispenser to the shooting stage, a situation arises for heading flag (on May 20th, 2005) investigation wheat powdery mildew.Respectively handle the state of an illness according to above stage division record, calculate the incidence of disease, disease index, relative control effect, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Figure C200610011591D00291
Figure C200610011591D00292
But the own azoles alcohol of table 14 44% tmtd wet-milling is to the control efficiency of wheat powdery mildew
As known from Table 14, but adopt the own azoles alcohol of 44% tmtd wet-milling spraying (600,900,1200 times) to wheat during jointing stage and heading stage powdery mildew good control efficiency (being respectively 81.0%～88.9% and 77.2%～84.5%) is arranged, but be significantly higher than the preventive effect of 6% penta 600 times of good fortune wet-millings, 600 times of 50% tmtd, 1200 times of 50% carbendazim.But the preventive effect that the own azoles alcohol of 44% tmtd wet-milling is 600 times is significantly higher than the preventive effect of 600 times of 5% hexaconazole microemulsions.
The own azoles alcohol of 6.44% tmtd is prevented and treated the wheat rust test of pesticide effectiveness
" bactericidal agent for preventing and treating cereal class rust test of pesticide effectiveness criterion " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion (two) ".The field production wheat breed is local susceptible variety " Handan 9587 ".The experimental field is positioned at south, Xu Cun township, south, Guanyao County, Hebei province Xu Cun, sowing on October 3rd, 2004, spacing in the rows 20cm, line-spacing 50cm.Wheat rust takes place over the years.Experimental field water and fertilizer condition is better, and mu is executed the chicken manure 3m that becomes thoroughly decomposed before the plantation 3, diammonium phosphate 20kg.The cultivation management term harmonization, the Pass Test requirement.Test medicine: the own azoles alcohol of 44% tmtd wetting powder.Dispenser dosage is 1200 times of (750g/hm 2), 900 times of (1000g/hm 2), 600 times of (1500g/hm 2).Contrast medicament: 750 times of (1200g/hm of 5% hexaconazole microemulsion 2); But 1200 times of (750g/hm of 50% carbendazim wet-milling 2); But 50% tmtd wet-milling; But 6% penta 600 times of (1500g/hm of good fortune wet-milling 2) 600 times of (1500g/hm 2), blank spraying clear water.Randomized arrangement is adopted in the experimental plot.If 3 processing of test medicine, 4 processing of contrast medicament, 1 processing of blank, 4 repetitions, totally 32 sub-districts.Sub-district area 20m 2, establish guard row.Turn green to the shooting stage at wheat, rust sporadicly takes place and (on March 15th, 2004 and March 30) beginning medication when laterally spreading, shared medicine 2 times, and be 15 days interval.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, reach evenly thoughtful during spraying.With amount of liquid medicine is 900L/hm 2No harsh weather influence during the whole test, temperature 12-24.5 ℃, in early April, 2005 and difference rainfall in the first tenday period of a month in May 1 time, rainfall is respectively 23mm and 29mm, and environmental condition is suitable for the generation of wheat rust.Experimental field physical features is smooth, and soil property is a clay, inclined to one side alkali, and soil fertility is higher.A preceding stubble harvest corn experimental field waters 1 time preceding 15 days (on September 17th, 2004), carries out no-tillage seeding behind the harvesting corn.Wheat (March 17) the big water logging of turning green the shooting stage is irritated 1 time, and every mu imposes urea 10kg with watering, and soil moisture is higher, helps rust and takes place.Before the sowing not with medicament handle seed, other bactericide diseases prevention of duration of test, but used 6000 times of (125g/hm of 50% Aphox wetting powder on March 20th, 2005 2) spray the control wheat aphid, spray 5000 times of (150g/hm of tribenuron-methyl 2) prevent and kill off (the spraying liquid amount 750L/hm of weeds in the ground 2).Every sub-district is selected representational or 5 sampling surveys of diagonal at random at 5, every some investigation 20 strains, three leaves in every strain investigation top (if having boot leaf then to comprise boot leaf), the percentage classification that accounts for whole leaf area with lesion area on the every leaf.Stage division: 0 grade: anosis; 1 grade: lesion area accounts for below 5% of whole blade area; 3 grades: lesion area accounts for 6%～25% of whole blade area; 5 grades: lesion area accounts for 26%～50% of whole blade area; 7 grades: lesion area accounts for 51%～75% of whole blade area; 9 grades: lesion area accounts for more than 76% of whole blade area.If other leaf diseases and fringe portion disease take place in the experimental plot, also must investigate and be illustrated.It is very light and even to fall ill before the dispenser for the first time, and state of an illness radix is designated as zero.Investigation will a situation arises, the lasting period of medicament according to disease for the first time, and 7 days (on April 8th, 2005, shooting stage) carried out after the dispenser the last time.Respectively handle the state of an illness according to above stage division record, calculate the incidence of disease, disease index, relative control effect, The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Figure C200610011591D00301
As known from Table 15, but adopt the own azoles alcohol of 44% tmtd wet-milling spraying (600,900,1200 times) that wheat leaf rust is had good control efficiency (82.2%～90.8%), be significantly higher than the preventive effect of 600 times of contrast medicament 50% tmtd, 1200 times of 50% carbendazim.But the preventive effect that the spraying of the own azoles alcohol of 44% tmtd wet-milling is 600 times is significantly higher than the preventive effect of 600 times of contrast medicament 5% hexaconazole microemulsions, but but the preventive effect of 600 times of the preventive effect of 900～1200 times of the own azoles alcohol of 44% tmtd wet-millings and 600 times of 5% hexaconazole microemulsions and 6% penta good fortune wet-millings is suitable, there was no significant difference, but but but the preventive effect of 600 times of the own azoles alcohol of 44% tmtd wet-millings is significantly higher than the preventive effect of 600 times of 6% penta good fortune wet-millings.
The own azoles alcohol of table 15 44% tmtd wetting powder is to the control efficiency of wheat leaf rust
Figure C200610011591D00303
Figure C200610011591D00311
The present invention studies confirm that pure being mixed with tmtd of own azoles has obvious synergistic effect to rhizoctonia cerealis, and two medicines are the strongest with the synergistic effect that 1:10 is mixed.Become the own azoles alcohol of 44% tmtd wetting powder, the own azoles alcohol of 44% tmtd suspension seed-coating agent according to this best proportioning preparation, every physical and chemical index is all up to standard.Pot experiment confirms that there is preventive effect preferably on 600～1200 times of sprayings of 44% tmtd own azoles alcohol wetting powder blade face to wheat powdery mildew, and in addition, this medicine has therapeutic action to wheat powdery mildew.Field trial shows that 600～1200 times of sprayings of the own azoles alcohol wetting powder of 44% tmtd have good preventive and therapeutic effect to wheat sharp eyespot, wheat powdery mildew and wheat leaf rust.The own azoles of 44% tmtd alcohol suspension seed-coating agent (22,33,44,66ga.i./100kg seed) seed dressing has certain preventive and therapeutic effect to root rotof flax, wheat sharp eyespot and take-all.According to observations, the own azoles alcohol of test medicine 44% tmtd wetting powder, the above concentration of the own azoles alcohol suspension seed-coating agent of 44% tmtd have no adverse effects to wheat and other beneficial organism, and it is obvious that wheat is protected the product effect.Suggestion uses the spraying of the own azoles alcohol of 44% tmtd wetting powder 600～1200 dilutions to handle wheat blade face and stem foot, dispenser liquid 900L/hm in the production 2Use the own azoles of 44% tmtd alcohol suspension seed-coating agent seed dressing (22,33,44,66ga.i./100kg seed) back sowing, the main disease of wheat there is the better prevention effect, but but but is better than conventional medicine 50% carbendazim wet-milling, 50% tmtd wet-milling, 6% Garrick show suspension seed-coating agent, 5% hexaconazole microemulsion and 6% penta good fortune wet-millings.

Claims (7)

1, a kind of composite bactericide of preventing and treating wheat fungal diseases or/and auxiliary agent is formed, be is characterized in that by active component, filler described active component is made up of the pure and mild tmtd of own azoles, and weight ratio is 1～10:10～50.
2, prevent and treat the composite bactericide of wheat fungal diseases according to claim 1, it is characterized in that, the weight ratio of the pure and mild tmtd of described own azoles is 1:5～15.
3, prevent and treat the composite bactericide of wheat fungal diseases as claimed in claim 1 or 2, it is characterized in that, when described composite bactericide was made wetting powder, it comprised following component and content:
1～10 part of own azoles alcohol
10～50 parts of tmtd
1～8 part of wetting agent
1～8 part of dispersant
10～80 parts of fillers;
Described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, lignosulfite, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex; Described wetting agent is a kind of in pelopon A, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate; Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite.
4, prevent and treat the composite bactericide of wheat fungal diseases as claimed in claim 1 or 2, it is characterized in that when described composite bactericide was made water dispersible granules, it comprised following component and content:
1～10 part of own azoles alcohol
10～50 parts of tmtd
2～8 parts of wetting agents
2～8 parts of dispersants
10～80 parts of fillers
5～10 parts of water and/or binding agents;
Described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, lignosulfite, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex; Described wetting agent is a kind of in pelopon A, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate; Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite; Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic, the polyvinyl alcohol.
5, prevent and treat the composite bactericide of wheat fungal diseases as claimed in claim 1 or 2, it is characterized in that when described composite bactericide was made suspension seed-coating agent, it comprised following component and content:
1～10 part of own azoles alcohol
10～50 parts of tmtd
2～8 parts of wetting agents
2～8 parts of dispersants
2～8 parts of suspension aids or binding agents
0.1～1 part of anti-bubble auxiliary agent
30～60 parts in water;
Described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, lignosulfite, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex; Described wetting agent is a kind of in pelopon A, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate; Described anti-bubble auxiliary agent is a kind of among silicone anti-foaming agent M10 or the anti-foaming agent C; Described binding agent is one or more in carboxymethyl cellulose, gum Arabic, cyclodextrin or the polyvinyl alcohol; Described suspension aids is an aluminium-magnesium silicate.
6, the composite bactericide of control control wheat fungal diseases as claimed in claim 1 or 2 is characterized in that, makes when doing outstanding agent when described composite bactericide, and it comprises following component and content:
1～10 part of own azoles alcohol
10～50 parts of tmtd
2～8 parts of wetting agents
2～8 parts of dispersants
2～8 parts of suspension aids or binding agents
2～8 parts of disintegrants
10～80 parts of fillers;
Described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, lignosulfite, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex; Described wetting agent is a kind of in pelopon A, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate; Described binding agent is one or more in carboxymethyl cellulose, gum Arabic, cyclodextrin or the polyvinyl alcohol; Described disintegrant is a kind of in bentonite, kaolin, diatomite, urea, sodium phosphate trimer or the sodium pyrophosphate; Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite; Described suspension aids is an aluminium-magnesium silicate.
7, as the application of composite bactericide in preparation control wheat sharp eyespot, wheat powdery mildew, wheat rust, take-all, root rotof flax and other wheat diseases agricultural chemicals of control wheat fungal diseases as described in the claim 1～6 any.
CNB2006100115918A 2006-03-29 2006-03-29 Compound bactericidal agent for prevention and control of wheat fungi diseases, and its application Expired - Fee Related CN100502653C (en)

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