CN100477913C - A compound bactericide for controlling plant powdery mildew - Google Patents
A compound bactericide for controlling plant powdery mildew Download PDFInfo
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- CN100477913C CN100477913C CNB2006100111298A CN200610011129A CN100477913C CN 100477913 C CN100477913 C CN 100477913C CN B2006100111298 A CNB2006100111298 A CN B2006100111298A CN 200610011129 A CN200610011129 A CN 200610011129A CN 100477913 C CN100477913 C CN 100477913C
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- Prior art keywords
- powdery mildew
- difenoconazole
- tpn
- cucumber
- test
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- 239000003899 bactericide agent Substances 0.000 title claims description 20
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- 239000005760 Difenoconazole Substances 0.000 claims description 78
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- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 4
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a pesticide for preventing plant powdery mildew, wherein the invention comprises active component, stuff and agent; the active component is formed by triazole pesticide and protector. The invention has 90% protecting effect and treatment effect, with low cost.
Description
Technical field
The present invention relates to pesticide field, relate to a kind of composite bactericide of preventing and treating plant powdery mildew particularly.
Background technology
The plant pathogenic fungi powdery mildew is absolute specialization bacterial parasite, infects behind the plant in the green portion expansion of plant, and its brood body hypergene except that absorbing plant nutrition, also influences photosynthesis of plants at the outside white powder layer that forms of plant, causes plant weak or dead.Powdery mildew of cucumber bacterium (Sphaerothecafuliginea) belongs to sac fungi, takes place throughout the year, and disease is easy to popular under high temperature, super-humid conditions.This germ parasitic scope is wide, relates to the melon crop of Denging.Adopt the chemical agent control in the agricultural production, as protectant tpn, tmtd, sulphur, carbendazim; Systemicss such as triazolone are used for the treatment of.Because the conidium of powdery mildew is propagated with air-flow, the bacterium amount is big, variation is fast, causes the medication that large watershed takes place, large-area control is anti-, a large amount of of disease.Because to prevent and treat disease medication number of times frequent, large tracts of land, high dose, use single systemics that the pesticide resistance of pathogen to medicament takes place easily for a long time.For example, after triazolone introducing Xinjiang was used for the control of Hami melon powdery mildew, effect descended rapidly in a short time.One of drug-fast solution route is that the medicament of the different modes of action is mixed, and changes the action site of medicament, produces new physiological change and the phenomenon no longer responsive to medicament to overcome after pathogen adapts to medicament.
Summary of the invention
(1) technical problem that will solve
The composite bactericide that the purpose of this invention is to provide the control plant powdery mildew of a kind of novel, efficient, safety, applied range.
(2) technical scheme
The composite bactericide of control plant powdery mildew of the present invention is characterized in that it is made up of active component, filler and auxiliary agent, and wherein, active component is made up of triazole bactericidal agent and protectant.
Composite bactericide of the present invention, the triazole bactericidal agent in the active component are selected from one or more in Difenoconazole, alkene azoles alcohol, Tebuconazole, nitrile bacterium azoles, the Flusilazole; Protectant is selected from one or more in tpn, the tmtd.
Composite bactericide of the present invention contain the combination of synergy effective amount of actives, and ratio is arbitrarily between active component, as long as can produce synergistic effect.
Generally speaking, the composition of active component is counted by ratio of weight and the number of copies: triazole bactericidal agent: protectant=1～10: 1～20.
Preferably, the composition of active component is counted by ratio of weight and the number of copies: triazole bactericidal agent: protectant=1: 4～16.
More preferably, the composition of active component is counted by ratio of weight and the number of copies: Difenoconazole: tpn=1: 4～12.
In composite bactericide of the present invention, described filler is one or more fillers that are selected from white carbon, kaolin, fine particle calcium carbonate, diatomite, bentonite, nitrous acid spent pulping liquor (in solids).
Composite bactericide of the present invention, adjuvant used this area usual auxiliaries that is is as sodium lignin sulfonate, sodium carboxymethylcellulose (CMC), neopelex, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), cyclohexanone, OP type emulsifier, ethylene glycol, ethylene glycol monobutyl ether, aluminium-magnesium silicate powder, polyoxy ethyl alkyl ether, Nonyl pheno base ether and sodium alkyl benzene sulfonate mixture.
Composite bactericide of the present invention can be mixed with various types of preparations according to technology well known in the art, for example wetting powder, dried outstanding agent granule, aqueous suspension agent, soluble powder, water dispersible granules, aqueous emulsion, microemulsion, emulsion etc.
50% Difenoconazole tpn wetting powder: take by weighing the former powder of enough Difenoconazoles and tpn, add suspension aids, dispersing aid and filler.After mixing stirring, enter one-level and pulverize (grain fineness reaches 50 mesh sieves); After row mixes stirring again, enter secondary and pulverize (grain fineness reaches 325 mesh sieves); Be packaged as product.Application process is spraying.Prevent and treat the powdery mildew of cucumber amount of application: 1000～2000 times of amount of liquid medicine 675L/hm
2(22.5～45g/667m
2).
50% Difenoconazole tpn is done outstanding agent: take by weighing the former powder of enough Difenoconazoles and tpn, add nitrous acid spent pulping liquor and OP emulsifier, mix and disperse, behind roller drying, carry out coarse crushing, be packaged as product.Application process is spraying.Prevent and treat the powdery mildew of cucumber amount of application: 1000～2000 times of amount of liquid medicine 675L/hm
2(22.5～45g/667m
2).
40% Difenoconazole tpn aqueous suspension agent: take by weighing the former powder of enough Difenoconazoles and tpn, add suspension aids and water.Each composition mixed after ball mill mixes pulverizes, enter sand mill then and pulverize and promptly obtain finished product.Application process is spraying.Prevent and treat the powdery mildew of cucumber amount of application: 1000～2000 times of amount of liquid medicine 675L/hm
2(22.5～45g/667m
2).
Composite bactericide of the present invention, the purposes in preventing and treating powdery mildew of cucumber, Hami melon powdery mildew, wheat powdery mildew, fruit tree powdery mildew and other plant powdery mildew.
(3) beneficial effect
The complex preparation synergistic effect of control plant powdery mildew of the present invention is remarkable, and effect is up to more than 90%, apparently higher than unitary agent.This complex preparation quick-acting and lasting effect are good, and mechanism of action and site are many, can effectively delay drug-fast generation of pathogen and development, thereby reduce the absolute dosage of agricultural chemicals, alleviate environmental pollution.Preparation technology is simple for this complex preparation, and cost is low, remarkable in economical benefits, and the diseases prevention spectrum is wide, the biological safety height.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
The preparation of embodiment 1 built wettable powder
50% Difenoconazole tpn (1: 10) wetting powder: get 4.75 parts of 96% Difenoconazoles, 49.4 parts of 92% tpns add 2 parts of sodium lignin sulfonates, 1.5 parts of CMC, 5 parts of neopelexes, 37.3 parts of fine particle calcium carbonates.After mixing stirring, enter one-level and pulverize (corase grind, grain fineness reaches 50 mesh sieves); After row mixes stirring again, enter secondary and pulverize (comminution by gas stream, grain fineness reach 325 mesh sieves); Be packaged as product.
The preparation of embodiment 2 built wettable powders
50% Difenoconazole tpn (1: 4) wetting powder: get 10.42 parts of 96% Difenoconazoles, 43.48 parts of 92% tpns add 2 parts of sodium lignin sulfonates, 1.5 parts of CMC, 5 parts of neopelexes, 37.6 parts of fine particle calcium carbonates.After mixing stirring, enter one-level and pulverize (grain fineness reaches 50 mesh sieves); After row mixes stirring again, enter secondary and pulverize (grain fineness reaches 325 mesh sieves); Be packaged as product.
The preparation of embodiment 3 built wettable powders
50% Difenoconazole tpn (1: 16) wetting powder: get 3.07 parts of 96% Difenoconazoles, 51.15 parts of 92% tpns add 2 parts of sodium lignin sulfonates, 1.5 parts of CMC, 5 parts of neopelexes, 37.28 parts of fine particle calcium carbonates.After mixing stirring, enter one-level and pulverize (grain fineness reaches 50 mesh sieves); After row mixes stirring again, enter secondary and pulverize (grain fineness reaches 325 mesh sieves); Be packaged as product.
The embodiment 4 composite preparations of doing outstanding agent
50% Difenoconazole tpn (1: 6) is done outstanding agent: get 7.44 parts of 96% Difenoconazoles, 46.58 parts of 92% tpns add nitrous acid spent pulping liquor (in solids) 40.98 parts, 5 parts of OP emulsifier.With former medicine, paper pulp concentrate and auxiliary agent mix disperse after, behind roller drying, carry out packing after the coarse crushing and get final product.
The preparation of embodiment 5 composite aqueous suspension agents
40% Difenoconazole tpn (1: 8) aqueous suspension agent: get 4.63 parts of 96% Difenoconazoles, 38.65 parts of 92% tpns, add 4 parts of ethylene glycol monobutyl ether, 3 parts of aluminium-magnesium silicate powder, 10 parts in polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and sodium alkyl benzene sulfonate mixture, 39.72 parts in water.Each composition mixed after ball mill mixes pulverizes, enter sand mill then and pulverize and promptly obtain finished product.
The preparation of embodiment 6 built wettable powders
50% Tebuconazole tpn (1: 20) wetting powder: get 2.51 parts of 95% Tebuconazoles, 51.76 parts of 92% tpns add 2 parts of sodium lignin sulfonates, 1.5 parts of CMC, 5 parts of neopelexes, 37.23 parts of fine particle calcium carbonates.After mixing stirring, enter one-level and pulverize (grain fineness reaches 50 mesh sieves); After row mixes stirring again, enter secondary and pulverize (grain fineness reaches 325 mesh sieves); Be packaged as product.
The embodiment 7 composite preparations of doing outstanding agent
50% triazolone tmtd (10: 20) is done outstanding agent: get 18.32 parts of 91% triazolones, 35.84 parts of 93% tmtd add nitrous acid spent pulping liquor (in solids) 40.84 parts, 5 parts of OP emulsifier.With former medicine, paper pulp concentrate and auxiliary agent mix disperse after, behind roller drying, carry out packing after the coarse crushing and get final product.
But experimental example 110% Difenoconazole dispersible granule is prevented and treated the powdery mildew of cucumber test of pesticide effectiveness
1 materials and methods
1.1 test material
1.1.1 bacterial strain: the powdery mildew of cucumber germ, pick up from suburb, Baoding.
1.1.2 chemical agent:
But 10% Difenoconazole dispersible granule provides by just reaching (China) Investment Co., Ltd earlier;
75% tpn wetting powder provides by just reaching (China) Investment Co., Ltd earlier
1.2 method:
Laboratory's pot experiment is selected the cucumber plant in anosis 4 Ye Miao mountain ranges, and quantitatively inoculation sprays soup after 2 days, every processing plant 20 basins.
Handle:
But 2000 times of 10% Difenoconazole dispersible granules;
But 3000 times of 10% Difenoconazole dispersible granules;
But 4000 times of 10% Difenoconazole dispersible granules;
800 times of 75% tpn wetting powders;
Blank.
2 results and analysis
But table 110% Difenoconazole dispersible granule is prevented and treated the powdery mildew of cucumber test
| Chemicals treatment | Sick leaf rate (%) | Disease index | Relative control effect (%) |
| But 2000 times of 10% Difenoconazole dispersible granule dilutions | 3.3 | 1.65 | 92.41 |
| But 3000 times of 10% Difenoconazole dispersible granule dilutions | 4.8 | 2.40 | 88.96 |
| But 4000 times of 10% Difenoconazole dispersible granule dilutions | 5.5 | 3.57 | 83.57 |
| 800 times of 75% tpn wettable powder dilution agents | 11.42 | 13.82 | 36.47 |
| Blank | 35.50 | 21.74 |
Result of the test shows, reaches 83%～92% but with 2000～4000 times of soups of 10% Difenoconazole dispersible granule powdery mildew of cucumber is carried out control efficiency, is significantly higher than the effect of 100 times of 75% tpn wetting powders.The test state of an illness is heavier, and the reliability of medicament to disease control has been described.
Experimental example 240% tpn medicament is to the control efficiency test of powdery mildew of cucumber
1 materials and methods
1.1 test material
1.1.1 bacterial strain powdery mildew of cucumber germ is picked up from suburb, Baoding.
1.1.2 chemical agent
75% tpn wetting powder provides by just reaching (China) Investment Co., Ltd earlier;
40% carbendazol wettable powder is produced by Shandong Huayang Science ﹠. Technology Co., Ltd..
1.2 method
Laboratory's pot experiment selects the cucumber plant in anosis 4 Ye Miao mountain ranges to spray soup after 2 days, quantitatively inoculation, every processing plant 20 basins.
Handle:
1000 times of 75% tpn wetting powders;
800 times of 75% tpn wetting powders;
500 times of 75% tpn wetting powders;
800 times of 40% carbendazol wettable powders;
Blank.
2 results and analysis
Table 275% tpn wetting powder is prevented and treated the powdery mildew of cucumber test
| Chemicals treatment | Sick leaf rate % | Disease index | Control efficiency % |
| 1000 times of 75% tpn wetting powders | 38.1 | 7.3 | 48.6 |
| 800 times of 75% tpn wetting powders | 28.6 | 5.4 | 61.8 |
| 500 times of 75% tpn wetting powders | 24.5 | 4.2 | 70.4 |
| 800 times of 40% carbendazol wettable powders | 29.2 | 5.4 | 62.4 |
| The clear water contrast | 50.5 | 14.2 | - |
The result shows that protectiveness medicament tpn has drug effect preferably to powdery mildew of cucumber, and 500～1000 times of soup preventive effects are 48%-70%, and is relatively poor aspect the disease controlling development.
Experimental example 3 different bactericide are tested the toxicity test of powdery mildew of cucumber
1 materials and methods
1.1 test material
1.1.1 bacterial strain powdery mildew of cucumber germ is picked up from suburb, Baoding.
1.1.2 chemical agent
96% Difenoconazole (production of Jiangsu ploughing and weeding chemical industry Co., Ltd);
95% Tebuconazole (production of Jiangsu Province Yancheng Lvye Chemical Co., Ltd.);
91% triazolone (production of Jiangsu Province Jianhu chemical industry Co., Ltd);
98% is suitable when happy (production of Jiangsu LiMin chemical engineering Co.,Ltd);
92% tpn (production of Jiangsu LiMin chemical engineering Co.,Ltd);
40% penta good fortune WP (commercially available);
25% metalaxyl frost urea cyanogen WP (commercially available);
50% sulphur triazolone WP (commercially available);
The WP of sulphur more than 50% (commercially available).
1.2 method
Adopt cotyledon method laboratory to measure: the configuration medicament is a series concentration: 200,100,50,25,12.5,6.25,3.125,1.625,0.81,0 μ g/ml.Potted plant cucumber midnight seedling plant, every processing plant 10 basins.Quantitatively inoculate at the cotyledon center, spray soup after 2 days, by the 4 grade standards investigation state of an illness, add up disease index and EC after 10 days
50Value.
2 results and analysis
The different bactericide of table 3 are to the toxicity test of powdery mildew of cucumber
| Handle | Virulence equation Y=a+bX | EC 50 μg/ml | R 2 |
| Difenoconazole | Y＝4.3420+1.2784X | 3.271 | 0.8516 |
| Tebuconazole | y＝3.8935+1.3122x | 6.9701 | 0.8617 |
| Triazolone | y＝-2.9666+2.2021x | 22.24226 | 11.288354.8803 |
| When suitable happy | y＝-0.0032+2.7939x | 61.7273 | 0.6172 |
| Tpn | y＝-10.2665+3.6254x | 678.86089 | 418.23441793.9861 |
| Tmtd | y＝2.0284+0.8129x | 4524.3291 | 0.8448 |
| 40% penta good fortune WP | y＝3.6611+0.8649x | 35.3216 | 0.9326 |
| 25% metalaxyl frost urea cyanogen WP | y＝0.6981+1.8182x | 232.4538 | 0.4650 |
| 50% sulphur triazolone WP | y＝1.0834+1.3921x | 650.7992 | 0.9002 |
| The WP of sulphur more than 50% | y＝3.2028+0.5481x | 1900.919 | 0.3085 |
Determination test is the result show, is that the triazole type medicament of representative has higher (EC to powdery mildew of cucumber with the Difenoconazole
50Be worth lower) virulence, and the virulence of protectiveness medicament tpn is lower.Both are used in combination and can play the effect that protective plant is not subjected to the PD of infection process, control generation the mode of action difference of pathogen, also can reach simultaneously and reduce the purpose of pathogen to the resistance risk of medicament.
The synergy determination test of experimental example 4 medicaments combination
1 materials and methods
1.1 test material
1.1.1 bacterial strain: the powdery mildew of cucumber germ, pick up from suburb, Baoding.
1.1.2 chemical agent: 96% Difenoconazole (production of Jiangsu ploughing and weeding chemical industry Co., Ltd);
92% tpn (production of Jiangsu LiMin chemical engineering Co.,Ltd).
1.2 method
Adopting cotyledon method laboratory to measure the configuration medicament is series concentration: 200,100,50,25,12.5,6.25,3.125,1.625,0.81,0 μ g/ml.Potted plant cucumber midnight seedling plant, every processing plant 10 basins.Quantitatively inoculate at the cotyledon center, spray soup after 2 days, by the 4 grade standards investigation state of an illness, add up disease index and EC after 10 days
50Value and coefficient of synergism.
2 results and analysis
Table 4 Difenoconazole and tpn are measured the synergy of powdery mildew of cucumber bacterium
| Handle | Y＝a+bX | EC 50Investigation value μ g/ml | EC 50Theoretical value | Coefficient of synergism SR | R 2 |
| Difenoconazole | Y＝4.3420+1.2784X | 3.271 | 0.8516 | ||
| Tpn | Y＝2.1024+1.0085X | 746.755 | 0.7978 | ||
| Difenoconazole: tpn=1: 1 | Y＝4.2185+0.9319X | 6.896 | 6.513 | 0.994 | 0.9769 |
| Difenoconazole: tpn=1: 2 | Y＝3.0974+1.4820X | 19.222 | 9.770 | 0.508 | 0.9264 |
| Difenoconazole: tpn=1: 4 | Y＝3.3510+1.4222X | 6.427 | 16.284 | 2.534 | 0.9377 |
| Difenoconazole: tpn=1: 6 | Y＝3.1109+1.7285X | 12.385 | 22.797 | 1.840 | 0.9643 |
| Difenoconazole: tpn=1: 8 | Y＝3.1400+1.5969X | 14.613 | 29.311 | 2.001 | 0.9172 |
| Difenoconazole: tpn=1: 10 | Y＝4.1286+0.9660X | 7.987 | 35.824 | 4.485 | 0.8867 |
| Difenoconazole: tpn=1: 12 | Y＝1.3140+3.2755X | 20.531 | 42.476 | 2.068 | 0.9429 |
| Difenoconazole: tpn=1: 14 | Y＝0.9496+3.5002X | 37.966 | 49.011 | 1.291 | 0.9201 |
| Difenoconazole: tpn=1: 16 | Y＝1.1227+3.1730X | 42.396 | 55.545 | 1.310 | 0.9926 |
| Difenoconazole: tpn=1: 18 | Y＝0.9845+3.2201X | 64.257 | 62.080 | 0.966 | 0.9730 |
| Difenoconazole: tpn=1: 20 | Y＝0.8378+3.4440X | 71.985 | 68.615 | 0.953 | 0.9290 |
Medicament Difenoconazole and tpn show in the simultaneous determination result of the test of 1: 1～1: 20 scope, 1: 4～1: 16 scope proportioning the virulence of powdery mildew of cucumber there is synergistic effect (synergism value is synergy more than 1.5), wherein with proportioning the best of 1: 10, this result provided reliable foundation for the development and the application of medicament.
Experimental example 5 combination medicament indoor harmacological effects
1 materials and methods
1.1 test material
1.1.1 bacterial strain powdery mildew of cucumber germ is picked up from suburb, Baoding.
1.1.2 chemical agent 50% Difenoconazole tpn wetting powder laboratory processed sample
1.2 method (providing concrete test method)
Laboratory's pot experiment is quantitatively inoculated the cucumber plant of 4 leaf seedling ages, sprays soup after the morbidity, or sprays soup after 2 days, quantitatively inoculation, every processing plant 20 basins.7-10 days investigation state of an illness after the medication.
Handle: 1500 times, 1000 times 500 times of 50% Difenoconazole tpn wetting powders.
The contrast medicament: 1000 times of 10% triazolone missible oil, Jianhu insecticide factory in Jiangsu produces.
2 results and analysis
2.1 protection effect to powdery mildew of cucumber
Table 5
Adopt 50% Difenoconazole tpn wetting powder protection cucumber leaves.Inoculate after the medication for the first time, the newborn blade incidence of disease of plant is extremely low, and state of an illness rank is very light; For the second time, new sick leaf does not appear after the medication for the third time; The contrast triazolone also has new sick leaf after the secondary medication.
2.2 result of treatment to powdery mildew of cucumber
Table 6
Inoculation morbidity back adopts 50% Difenoconazole tpn wetting powder to spray cucumber plant, the result of treatment of powdery mildew is improved according to the raising of spraying concentration, and improve along with the increase of medication number of times.Imitate the result who is significantly higher than the triazolone same period.The sick leaf of relative comparison handles making that the pathogen bacterium colony subsides, variable color to mycelia disappears.
Experimental example 6 field trials
One, test objective
Checking Mixed Pharmacy 50% Difenoconazole tpn wetting powder is to the best field using dosage of powdery mildew of cucumber, and the test medicament indicates benefit biological influence, medicament drug effect and safe and reasonable to crop and non-handle, determines operation technique.
Two, experimental condition
1. trial crops and target
Trial crops is a cucumber, and kind is green No. 3 of Tianjin
2. subjects
Powdery mildew of cucumber bacterium (Sphaerotheca fuliginea)
3. environmental condition
Test site is positioned at suburb, Baoding Lang Zhuan protection ground booth, plants cucumber year after year, and powdery mildew takes place throughout the year, and preceding crop is a cucumber, and physical features is smooth, and soil property is a loam, and water and fertilizer condition is better, and mu is executed chicken manure 3m before the plantation
3The cultivation condition uniformity, the Pass Test requirement.
Three, experimental scheme and arrangement
If 3 processing of test medicine, 2 processing of contrast medicament, 1 processing of blank.Arrange in field district's group at random, four repetitions, natural occurrence.
1. medicament
1.1 test medicine and treatment dosage
Test medicine: 50% Difenoconazole tpn wetting powder laboratory processed sample
Chemicals treatment is: 2000 times, 1500 times, 1000 times of 50% Difenoconazole tpn wetting powders
1.2 contrast medicament
The contrast medicament: but 2000 times of 10% Difenoconazole dispersible granules; Syngenta Co.,Ltd produces;
800 times of 75% tpn wetting powders, Syngenta Co.,Ltd produces.
If blank.
2. arrange the sub-district
2.1 arrange the sub-district: randomized arrangement is adopted in the experimental plot.
2.2 sub-district area and repetition
Sub-district area 21M
2, 4 repetitions, totally 24 sub-districts.
3. insecticide-applying way
3.1 spraying time and number of times
Use medicine (April 8) for the first time during field first meeting scab, again respectively at April 18, continuous dispenser on April 28.
3.2 use apparatus and application method:
Adopt Jacto-40 type knapsack hand sprayer spray pesticide, spraying is evenly thoughtful, is blank with the clear water.With amount of liquid medicine is 675L/hm
2
3.3 meteorological data
Duration of test does not have the harsh weather influence, and 23～35 ℃ of temperature of shed watered once in per 5～7 days, and air humidity is bigger in the canopy, is suitable for the generation of disease.
Four, investigation
1. investigation method and grade scale
Investigate the state of an illness of the newborn blade of cucumber plant and the PD of the disease leaf of having fallen ill respectively.Every sub-district is got 5 investigation at random, every some investigation 3 strains, and 5～10 blades are investigated in every strain from top to bottom, account for the percentage classification of whole leaf area by scab on the blade.
Grade scale:
0 grade: no scab;
1 grade: scab accounts for below 5% of whole leaf area;
3 grades: scab accounts for 6～10% of whole leaf area;
5 grades: scab accounts for 11～25% of whole leaf area;
7 grades: scab accounts for 26～50% of whole leaf area;
9 grades: scab accounts for more than 50% of whole leaf area.
2. control time
State of an illness radix is zero (April 8) before the dispenser; 10 days (May 5) investigation incidence after the medication for the third time.
3. drug effect computational methods
Field investigation is respectively handled the disease number of sheets and is write down the state of an illness, calculates disease index, relative control effect according to above stage division, and The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Five, result and analysis
Table 7
1. by table as can be seen, field control effect to the powdery mildew of cucumber bacterium after 2000 times, 1500 times, 1000 times continuous three medications of three treatment dosage of 50% Difenoconazole tpn wetting powder is respectively 97.40%, 100% and 100%, though control efficiency concentration increases and improves, difference is not remarkable between the three; But 2000 times of contrast medicament 10% Difenoconazole dispersible granules and 800 times of field control effects to the powdery mildew of cucumber bacterium of 75% tpn wetting powder are respectively 97.4% and 78.0%; But difference is not obvious between the control efficiency of control efficiency that 10% Difenoconazole dispersible granule is 1000～1500 times and test medicine 50% Difenoconazole tpn wetting powder treatment dosage; All be significantly higher than the control efficiency of 800 times of 75% tpn wetting powders.
2. according to observations, the above dosage of test medicine does not have the poisoning phenomenon to cucumber growth, and is harmless to other biology.
3. binary mixture preparation 50% Difenoconazole tpn wetting powder has the better prevention effect to the powdery mildew of cucumber bacterium, can use in the field, the suggestion working concentration is 1000～2000 times, and their early stage is sprayed, general logotype three times, the medication interval phase is 7～10 days.
Experimental example 7 field trials
One, test objective
Checking Mixed Pharmacy 50% Difenoconazole tpn wetting powder is to the best field using dosage of wheat powdery mildew, and the test medicament indicates benefit biological influence, medicament drug effect and safe and reasonable to crop and non-handle, determines operation technique.
Two, experimental condition
1. trial crops and target
Trial crops is a wheat, and kind is a wheat No. 9 in the susceptible production kind
2. subjects
Wheat powdery mildew (Erysiphe graminis)
3. environmental condition
Test site is positioned at Baoding Rongcheng, plants wheat year after year, and powdery mildew takes place throughout the year, and preceding crop is a corn.Physical features is smooth, and soil property is a loam, and water and fertilizer condition is better, and planting density is a mu spike number 380,000, and on October 3 date of seeding, line-spacing is 0.25m.The cultivation condition uniformity, management Pass Test requirement.Trial crops breeding time is that the wheat jointing is to the pustulation period.
Three, experimental scheme and arrangement
If 3 processing of test medicine, 2 processing of contrast medicament, 1 processing of blank.Arrange in field district's group at random, four repetitions, natural occurrence.
1. medicament
1.1 test medicine and treatment dosage
Test medicine: 50% Difenoconazole tpn wetting powder laboratory processed sample;
Chemicals treatment is: 2000 times, 1500 times, 1000 times of 50% Difenoconazole tpn wetting powders.
1.2 contrast medicament
The contrast medicament: but 2000 times of 10% Difenoconazole dispersible granules; Syngenta Co.,Ltd produces;
800 times of 75% tpn wetting powders, Syngenta Co.,Ltd produces.
If blank.
2. arrange the sub-district
2.1 arrange the sub-district: randomized arrangement is adopted in the experimental plot.
2.2 sub-district area and repetition
Sub-district area 15M
2, 4 repetitions, totally 24 sub-districts.
3. insecticide-applying way
3.1 spraying time and number of times
During field first meeting on May 5 (wheat grain filling phase) disease leaf, foliar spray 1～2 time.
3.2 use apparatus and application method:
Adopt Jacto-40 type knapsack hand sprayer spray pesticide, spraying is evenly thoughtful, is blank with the clear water.With amount of liquid medicine is 675L/hm
2
3.3 meteorological data
Though duration of test does not have the harsh weather influence, spring drought influences the development of the state of an illness year after year.Fine during dispenser, calm no rain.
Four, investigation
1. investigation method and grade scale
Every sub-district is got 5 investigation at random, every some investigation 20 strains, and 3 blades are investigated in every strain from top to bottom, account for the percentage classification of whole leaf area by scab on the blade.
Grade scale:
0 grade: no scab;
1 grade: scab accounts for below 5% of whole leaf area;
3 grades: scab accounts for 6～10% of whole leaf area;
5 grades: scab accounts for 11～25% of whole leaf area;
7 grades: scab accounts for 26～50% of whole leaf area;
9 grades: scab accounts for more than 50% of whole leaf area.
2. control time
State of an illness radix is zero (May 5) before the dispenser; Investigation on 10 days (May 15) incidence after the medication.
3. drug effect computational methods
Field investigation is respectively handled the disease number of sheets and is write down the state of an illness, calculates disease index, relative control effect according to above stage division, and The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Five, result and analysis
Table 8
| Handle | Disease index | Control efficiency % |
| 2000 times of 50% Difenoconazole tpn wettable powders | 1.49 | 91.28 |
| 1500 times of 50% Difenoconazole tpn wetting powders | 0.56 | 96.72 |
| 1000 times of 50% Difenoconazole tpn wetting powders | 0.12 | 99.29 |
| But 2000 times of 10% Difenoconazole dispersible granules | 1.14 | 93.33 |
| 800 times of 75% tpn wetting powders | 4.19 | 75.49 |
| Blank | 17.10 | / |
1. by table as can be seen, field control effect to wheat powdery mildew after 2000 times, 1500 times, 1000 times medications of three treatment dosage of 50% Difenoconazole tpn wetting powder is respectively 91.28%, 96.72% and 99.29%, and control efficiency increases with concentration and improves; But 2000 times of contrast medicament 10% Difenoconazole dispersible granules and 800 times of field control effects to wheat powdery mildew of 75% tpn wetting powder are respectively 93.33% and 75.49%; But difference is not obvious between the control efficiency of the control efficiency that 10% Difenoconazole dispersible granule is 2000 times and 1000～1500 times of treatment dosage of test medicine 50% Difenoconazole tpn wetting powder; All be significantly higher than the control efficiency of 800 times of 75% tpn wetting powders.
2. according to observations, the above dosage of test medicine does not have the poisoning phenomenon to wheat growth, and is harmless to other biology.
3. binary mixture preparation 50% Difenoconazole tpn wetting powder has the better prevention effect to wheat powdery mildew, can use in the field, the suggestion working concentration is 1000～2000 times, and their early stage is sprayed, medication 1～2 time, the medication interval phase is 10～15 days.
Experimental example 8 field trials
One, test objective
Checking Mixed Pharmacy 50% Difenoconazole tpn wetting powder is to the best field using dosage of apple mildew, and the test medicament indicates benefit biological influence, medicament drug effect and safe and reasonable to crop and non-handle, determines operation technique.
Two, experimental condition
1. trial crops and target
Trial crops is an apple, and kind is the Fuji apple
2. subjects
Powdery mildew of cucumber bacterium (Sphaerotheca fuliginea)
3. environmental condition
Test site is positioned at apple orchard, suburb, Baoding, the age of tree 8 years; Experimental field physical features is smooth, and soil property is a loam, and water and fertilizer condition is better, and powdery mildew takes place throughout the year.
Three, experimental scheme and arrangement
If 3 processing of test medicine, 2 processing of contrast medicament, 1 processing of blank.Arrange in field district's group at random, four repetitions, natural occurrence.
1. medicament
1.1 test medicine and treatment dosage
Test medicine: 50% Difenoconazole tpn wetting powder, this laboratory processed sample;
Chemicals treatment is: 2000 times, 1500 times, 1000 times of 50% Difenoconazole tpn wetting powders.
1.2 contrast medicament
The contrast medicament: but 2000 times of 10% Difenoconazole dispersible granules, and Syngenta Co.,Ltd produces;
800 times of 75% tpn wetting powders, Syngenta Co.,Ltd produces.
If blank.
2. arrange the sub-district
2.1 arrange the sub-district: randomized arrangement is adopted in the experimental plot.
2.2 sub-district area and repetition
2 trees of sub-district area, 4 repetitions, totally 24 sub-districts.
3. insecticide-applying way
3.1 spraying time and number of times
During the fragmentary scab of first meeting with medicine (May 20) for the first time, dispenser for the second time on June 5.
3.2 use apparatus and application method:
Adopt Jacto-40 type knapsack hand sprayer spray pesticide, spraying is evenly thoughtful, is blank with the clear water.With amount of liquid medicine is 5L/ tree.
3.3 meteorological data
Duration of test does not have the harsh weather influence.
Four, investigation
1. investigation method and grade scale
Every tree is investigated 100 blades from top to bottom, accounts for the percentage classification of whole leaf area by scab on the blade.
Grade scale:
0 grade: no scab;
1 grade: scab accounts for below 5% of whole leaf area;
3 grades: scab accounts for 6～10% of whole leaf area;
5 grades: scab accounts for 11～25% of whole leaf area;
7 grades: scab accounts for 26～50% of whole leaf area;
9 grades: scab accounts for more than 50% of whole leaf area.
2. control time
State of an illness radix is zero (May 20) before the dispenser; Investigation on 10 days (June 15) incidence after the medication for the second time.
3. drug effect computational methods
Field investigation is respectively handled the disease number of sheets and is write down the state of an illness, calculates disease index, relative control effect according to above stage division, and The data Deng Kenshi duncan's new multiple range method (DMRT) carries out statistical analysis.
Five, result and analysis
Table 9
| Handle | Disease index | Control efficiency % |
| 2000 times of 50% Difenoconazole tpn wettable powders | 1.31 | 91.03 |
| 1500 times of 50% Difenoconazole tpn wetting powders | 0.88 | 93.97 |
| 1000 times of 50% Difenoconazole tpn wetting powders | 0.43 | 97.05 |
| But 2000 times of 10% Difenoconazole dispersible granules | 0.94 | 93.56 |
| 800 times of 75% tpn wetting powders | 4.39 | 69.93 |
| Blank | 14.60 | / |
1. by table as can be seen, field control effect to the apple mildew bacterium after 2000 times, 1500 times, 1000 times continuous quadratic medications of three treatment dosage of 50% Difenoconazole tpn wetting powder is respectively 91.03%, 93.97% and 97.05%, control efficiency increases with concentration and improves, and difference is not remarkable between the three; But 2000 times of contrast medicament 10% Difenoconazole dispersible granules and 800 times of field control effects to the apple mildew bacterium of 75% tpn wetting powder are respectively 93.56% and 69.93%; But difference is not obvious between the control efficiency that control efficiency that 10% Difenoconazole dispersible granule is 2000 times and test medicine 50% Difenoconazole tpn wetting powder are 1000～1500 times; All be significantly higher than the control efficiency of 800 times of 75% tpn wetting powders.
2. according to observations, the above dosage of test medicine does not have the poisoning phenomenon to apple development, and is harmless to other biology.
3. binary mixture preparation 50% Difenoconazole tpn wetting powder has the better prevention effect to the apple mildew bacterium, can use in the field, the suggestion working concentration is 1000～2000 times, and their early stage is sprayed, general logotype three times, the medication interval phase is 10～20 days.
Claims (3)
1, a kind of composite bactericide of preventing and treating plant powdery mildew is characterized in that it is made up of active component, filler and auxiliary agent, and the composition of wherein said active component is counted by ratio of weight and the number of copies: Difenoconazole: tpn=1: 4～12.
2, composite bactericide as claimed in claim 1 is characterized in that filler is selected from one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite, bentonite, the nitrous acid spent pulping liquor.
3, the purposes of composite bactericide as claimed in claim 1 or 2 in preventing and treating powdery mildew of cucumber, Hami melon powdery mildew, wheat powdery mildew, fruit tree powdery mildew.
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| US9364001B2 (en) | 2013-11-07 | 2016-06-14 | Adama Makhteshim Ltd. | Quaternary fungicidal mixture |
| US10729137B2 (en) | 2013-11-07 | 2020-08-04 | Adama Makhteshim Ltd. | Quaternary fungicidal mixture |
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