CN102919256A - Fluopicolide-containing compound bactericide and application thereof - Google Patents
Fluopicolide-containing compound bactericide and application thereof Download PDFInfo
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Abstract
The invention provides a fluopicolide-containing compound bactericide which comprises active ingredients and auxiliary agents, wherein the active ingredients comprise fluopicolide and strobilurin bactericide with the weight ratio of 1-10: 1-90; and the strobilurin bactericide comprises one or more of pyraclostrobin, azoxystrobin, pyrametostrobin, enestroburin, pyraoxystrobin, flufenoxystrobin. The fluopicolide-containing compound bactericide is remarkable in synergism in the aspect of preventing and controlling fruit and vegetable oomycetes diseases. The compound bactericide is good in fast-acting property and persistence, unique in mechanism of action and multiple in acting sites, and can effectively delay the occurrence and development of bactericide resistance of pathogenic bacteria. The compound preparation is simple in preparation technology, low in cost, remarkable in economical benefit, wide in disease prevention spectrum and high in biosecurity.
Description
Technical field
The invention belongs to pesticide field, be specifically related to a kind of biocide that contains heterocyclic compound and preparation method thereof.
Background technology
Fruits and vegetables oomycetes disease be infected by the Mastigomycotina oomycetes due to, wherein, downy mildew mould take epidemic disease, the rotten oomycetes such as mould are the important pathogen of fruits and vegetables, the vegetable crops such as the fruit tree crops such as main harm grape and Cruciferae, Solanaceae, Curcurbitaceae, cause damping off, pythium rot, epidemic disease, cotton disease, downy mildew and the root rot etc. of fruits and vegetables, cause the significantly underproduction even total crop failure
[1]Especially downy mildew and phytophthora short incubation period infect often again, and germ can develop rapidly and cause disease popular within the season of growth of plant, causes the heavy losses of agricultural production.Can in several days, cause cucumber plant all dead such as cucumber downy mildew
[2]Potato late blight occured greatly in Ireland in 1847, caused potato in poor harvest, and the large famine that causes causes nearly million people dead, and more than 200 ten thousand people wander alien land
[3]At present chemical control is the Main Means of control oomycetes disease and the important leverage of crop high yield.The bactericide of control fruits and vegetables oomycetes disease mainly contains protectant and systemics in the production.
Protective fungicide.Representative products is mancozeb, Carbatene, tpn, Kocide SD etc.After being applied in plant surface; contact the first infection processs of prevention with germ by residual activity and reach the purpose of preventing and treating disease; although fungicidal spectrum is wide; be difficult for developing immunity to drugs; but can only before occuring, disease use; invalid to the pathogen in the invaded plants body, can not play effective protective effect to newborn branches and leaves
[4,5]
Systemic fungicide refers to and can be entered plant corpus by the leaf of plant, stem, root absorption, and in plant corpus transporting to the bactericide of site of action
[6], this class medicament can protective plant cambium, has overcome protective fungicide and can only prevent the shortcoming that can not treat and root out.The systemic fungicide of control oomycetes disease can be divided into following a few class:
The benzamide series bactericidal agent; representative products is fluopicolide; Plasmopara viticola, bacterium of downy mildew of cucumber, phytophthora infestans, tomato late blight bacterium and downy mildew of lettuce bacterium etc. are had than high bacteriostatic activity; its mechanism of action is to disturb the combination of class spectrin and other components; destroy the stability of cytoskeleton; the release, sporangial formation and sprouting and the mycelial growth that suppress zoospore have protection and therapeutic action
[19-20]The sharp SC of 68.75% silver medal method is the complex preparation of fluopicolide and Propamocarb, and the Oomycete diseases such as downy mildew are had remarkable preventive effect.
The phenyl amide series bactericidal agent comprises metalaxyl, Metalaxyl-M, furalaxyl, M 9834, ofurace and the spirit of Evil frost etc.This series bactericidal agent is invaded the inhibitory action of release, sprouting and intrusion of each stage before the host such as zoospore to germ not obvious, but to invading each stage behind the host such as growth, the haustorium of mycelia in plant corpus forms and sporangial generation etc. has significant inhibitory action
[7]The phenyl amide series bactericidal agent is general to be used with other bactericide (such as mancozeb, white urea cyanogen etc.), thunderous many Mils (mixture of metalaxyl and mancozeb), Sandofan (mixture of the clever and mancozeb of Evil frost), Jin Lei (mixture of Metalaxyl-M and mancozeb) etc.
Ethanamide bactericide, Representative Cultivars are white urea cyanogen, and its activity that suppresses the germ mycelial growth is higher than and suppresses zoospore and sprout, and to the unrestraint effect of moving about of respiration and zoospore, do not affect the generation of energy
[8]Normal and the protective fungicides such as mancozeb or copper agent of frost urea cyanogen are used with.Studies show that white urea cyanogen and mancozeb Ji the clever Ternary of Evil frost uses Phytophthora infestans that synergistic effect is arranged
[6]
Carboxylic acid amide series bactericidal agent, this series bactericidal agent are a kind of medicaments of novel structure, the formation of the synthetic interference cell wall by suppressing the germ phosphide, thus play the effect of kill pathogens
[9]Comprise mandipropamid, dimethomorph, flumorph and benzene metsulfovax etc., normal and the pharmacy mix such as mancozeb and tmtd play an important role in preventing and treating the oomycetes disease such as potato late blight, downy mildew of cucurbits and capsicum epidemic disease and the resistance of improvement to the phenyl amide series bactericidal agent
[10-13]
The QoI series bactericidal agent; comprise methoxy acrylic bactericide with the famoxadone series bactericidal agent; have the characteristics such as wide spectrum, efficient, interior suction; suppress germ spore germination, mycelial growth and sporulation; fruits and vegetables oomycetes disease and early blight, powdery mildew are had preferably control efficiency, infecting in early days of disease had good protection and result for the treatment of
[14]Methoxy acrylic bactericide comprises Fluoxastrobin, kresoxim-methyl, pyraclostrobin, SSF 126, ZEN 90160, fluoxastrobin, dimoxystrobin, orysastrobin, Enestroburin, alkene oxime amine and azoles amine bacterium ester etc.
[15]The Representative Cultivars of famoxadone series bactericidal agent is famoxadone, the complex preparation of major product Shi famoxadone and white urea cyanogen or mancozeb.
2,6-dinitroaniline series bactericidal agent, representative products are fluazinam, suppress the inside and outside proton conduction of mitochondrial membrane, belong to oxidative phosphorylation uncoupler
[16], having a longevity of residure long, the effect of shovel spore is strong, and the characteristics such as suction are higher to ash arrhizus bacteria, late disease bacteria and melon anthrax bacteria isoreactivity in the part.
Carbamate fungicide, representative products is Propamocarb, suppress the synthetic of germ fatty acid, destroy membrane passage, it is active that Phytophthora infestans, tomato late blight bacterium and bacterium of downy mildew of cucumber etc. have higher live body, but isolated activity is lower, suppresses the active relatively poor of sporangium sprouting
[17,18]
The imidazoles bactericide take cyazofamid as representative, acts on the mitochondria electronics and transmits complex compound III Qi position, is used for preventing and treating the oomycetes diseases such as downy mildew, epidemic disease.Growth of release, movement and the mycelia of the formation of the sporangial formation of phytophthora, sprouting, egg spore, zoospore etc. all had very high inhibitory action
[19,20]
Pesticide resistance generally occurs in the oomycetes disease, causes control efficiency to descend, and is badly in need of exploitation new bactericide and composite product thereof in the production.With systemics and protectant or be processed into mixture with the systemics of other different mechanisms of action and use and can enlarge fungicidal spectrum, disease is planted by the Zhiduo County of holding concurrently; Prolong the lasting period, reduce the medication number of times; Obtain synergistic effect, improve drug effect; Delay controlling object to the resistance generation of systemic fungicide.
So far there is not yet the report of the mixture that utilizes fluopicolide and pyraclostrobin development control oomycetes disease, also have no the mixture of the methoxy acrylic bactericides such as fluopicolide and pyraclostrobin to the research report of the control efficiency of fruits and vegetables oomycetes disease.
List of references:
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[2] Dong Jingao chief editor. Agricultural Plant Pathology. the north this [M]. Chinese agriculture publishing house, 2001.5.
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[4]Schwinn?F?J,Margot?P.Control?with?chemicals[A].Ingram?D?S,Williams?P?H.Phytophthora?infestans，the?cause?of?late?blight?ofpotato,Advances?in?Plant?Pathology(vol.7)[C].London:AcademicPress,1991,225-265.
[5]Fernandez?E?N,Navia?O,Gandarillas?A.Basis?of?strategies?forchemical?control?of?potato?late?blight?developed?by?PROINP?inBolivia[J].Fitopatologa,2000,35(3):137-149.
[6] Wang Wenqiao. gas passes downy mildew and epidemic disease is mould to the spirit of Evil frost, dimethomorph and white urea cyanogen resistance risk research [D] .2000, China Agricultural University.
[7] Lin Kongxun. bactericide toxicology [M]. Chinese agriculture publishing house, 1995,101-140.
[8]Ziogas,B?N,Davidse,L?C.Studies?on?the?mechanism?of?action?ofcymoxanil?in?Phytophthora?infestans[J].Pesticide?Biochemistry?andPhysiology,1987,29:89-96.
[9] inteilectual Zhu, Liu Xili, Li Jianqiang, etc. the Assimilation and conductivity behavior [J] of new type bactericide flumorph in cucumber plant. SCI, 2006,27 (10): 1887-1890.
[10] the yellow youth, Ye Zhongyin. the characteristic of dimethomorph (DMM) and mechanism of action [J] thereof. pesticide science and management, 2000,21 (5): 27-31.
[11] Chi Huiwei, Diao Jie, Nie Kaisheng, etc. new type bactericide mandipropamid [J]. agricultural chemicals, 2007,46 (1): 52-54.
[12] Wang Li, Zhu Jiehua, Wang Wenqiao, etc. novel germicide Phytophthora infestans Toxicity Determination [A]. grain security and plant protection scientific and technical innovation [C]. Scientia Agricultura Sinica technology publishing house, 2009,774-777.
[13] Han Xiuying, Li Hongmin, Ma Zhiqiang, etc. novel germicide AF-12946 is to the virulence and control effect [A] of potato late blight. Chinese Plants disease chemical control research [C]. and Scientia Agricultura Sinica technology publishing house, 2008,6:73-76.
[14] Yan Xiaojing, Jin Shuhui, Chen Fuheng waits the progress [J] of .Strobilurin series bactericidal agent action target. Pesticide Science journal, 2006,8 (4): 299-305.
[15] Qiao Guishuan. five kinds of Strobilurin series bactericidal agents are to bacterium of downy mildew of cucumber biologically active and resistance risk [D] thereof. Agricultural University Of Hebei, 2009.
[16]Guo?Z,Miyoshi?H,Komyoji?T.Uncoupling?activity?of?a?newlydeveloped?fungicide?fluazinam,[3-chloro-N-(3-chloro-2,6-dinitro-4-trifluoromethylphenyl)-5-trifluoromethyl-2-pyridinamine][J].Biochemical?Biophysics?Abstract,1991,1056:89-92.
[17]Yuan?H?Z,Qi?S?H,Jin?M.Control?of?downy?mildew?of?cucumberwith?propamocarb?aqueous?solution[J].Plant?Protection.1999.25(3):45-46.
[18]Reiter?B,Wenz?M,Buschhaus?H,et?al.Effect?of?propamocarbhydrochloride?on?Phytophthora?infestansinvitro?and?in?potato?andtomato[J].Gesunde?Pflanzen,1995,47(2):43-50.
[19] Liu Changling. world pesticide complete works-bactericide volume [M]. Chemical Industry Press, 2006:203-205.
[20]Takeshi?O,Terumasa?K,Shigeru?M.Development?of?a?novelfungicide,cyazofamid[J].Pesticide?Science,2004,29:136-138.
Summary of the invention
For the weak point in composite bactericide field, the object of the invention provides the composite bactericide of the control fruits and vegetables oomycetes disease of a kind of novel, efficient, safety, applied range.
Another object of the present invention provides the application of composite bactericide in control fruits and vegetables oomycetes disease.
The concrete technical scheme that realizes above-mentioned purpose of the present invention is:
A kind of composite bactericide of containing fluopicolide, it is comprised of active component and auxiliary agent, and described active component is comprised of fluopicolide and methoxy acrylic bactericide, and its weight ratio is 1～10:1～90.
Preferably, the weight ratio of described fluopicolide and methoxy acrylic bactericide is 1～7:1～20.
More preferably, the weight ratio of described fluopicolide and methoxy acrylic bactericide is 1 ~ 5:1～7.
Described methoxy acrylic bactericide is one or more the mixing in pyraclostrobin, Fluoxastrobin, azoles amine bacterium ester, Enestroburin, azoles bacterium ester, the fluorine bacterium mite ester.
Wherein, the formulation of described composite bactericide is soluble powder, soluble granula, water dispersant (WG), aqua, wetting powder (WP), soluble liquid, missible oil (EC), aqueous emulsion (AE), microemulsion (ME) or suspending agent (SC).
Described composite bactericide is comprised of the component of following weight portion:
When described composite bactericide was made wetting powder, it comprised following component and content:
Its preparation process is: according to said ratio, various compositions through mix and blend, are entered one-level and pulverize (grain fineness reaches 80 orders); After carrying out mix and blend again, enter secondary pulverizing (grain fineness reaches 325 orders) and make finished product.
When described composite bactericide was made water dispersible granules, it comprised following component and content:
Its preparation process is: according to said ratio fluopicolide, methoxy acrylic bactericide, dispersant, wetting agent and filler are passed through mix and blend; air-flow crushing obtains wetting powder; then wetting powder and water (or with binding agent) are added in the kneader simultaneously and mediate; make plastic material; send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.
Described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex.Specifically be selected from: one or more mixtures in sodium lignin sulfonate (M-9), desugar sodium lignin sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (NNO), 4-oleoyl-methoxy benzenesulfonic acid sodium salt (LS), sodium carboxymethylcellulose (CMC), lignosulfite, alkylphenol polyoxyethylene (OP-10), ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and the neopelex.
Described wetting agent is a kind of in neopelex, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate.Specifically be selected from wetting agent wgwin D30, nekal (Nekal BX), pelopon A, sodium alkyl benzene sulfonate, alkyl benzene calcium sulfonate, soapberry powder, bay alcohol radical sodium sulphate, fatty alcohol-polyoxyethylene ether (JFC) or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate (SOPA) one or more.
Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite; Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic, the polyvinyl alcohol.
Described thickener is one or more in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described anti-foaming agent is silicone anti-foaming agent M10 or anti-foaming agent C;
Described disintegrant is a kind of in bentonite, calcium chloride, anhydrous sodium sulfate, kaolin, diatomite, ammonium sulfate, urea, sodium phosphate trimer or the sodium pyrophosphate;
Described antifreezing agent is one or more the mixture in glycerine, urea, ethylene glycol, the propane diols.
The application of the composite bactericide that the present invention proposes in control fruits and vegetables oomycetes disease.
Wherein, described fruits and vegetables oomycetes disease comprise the downy mildew, white blister of fruit and/or vegetables, by the mould damping off that causes of corruption and stem rot, by the mould root rot that causes of epidemic disease, stem rot, base rot disease, fruit rot and cotton disease and late blight.
Beneficial effect of the present invention is:
The compositional bactericidal agent for preventing and treating fruits and vegetables oomycetes disease synergistic effect of the containing fluopicolide that the present invention proposes is remarkable.25% fluopicolide pyraclostrobin WP 112.5g a.i./hm of the present invention
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2The effect of (1000 times of liquid) spraying control cucumber downy mildew, potato late blight, downy mildew of garpe, tomato late blight, capsicum epidemic disease and Chinese cabbage downy mildew reaches 83.2%～95.3%, is better than 25% pyraclostrobin EC 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2The preventive effect of (1000 times of liquid).This composite bactericide quick-acting and lasting effect are good, and mechanism of action is unique, and action site is many, can effectively delay the drug-fast generation of pathogen and development.Preparation technology is simple for this complex preparation, and cost is low, remarkable in economical benefits, and the diseases prevention spectrum is wide, and biological safety is high.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.
Medicine in embodiment and the experimental example: the former medicine of 96% fluopicolide, the former medicine of 90% Enestroburin, the former medicine of 93% azoles bacterium ester, the former medicine of 95% fluorine bacterium mite ester, the former medicine of 87% azoles amine bacterium ester, the former medicine of 96% pyraclostrobin, Shenyang Chemical Engineering Inst; The former medicine of 95% Fluoxastrobin, gold soil, Nanjing chemical industry Co., Ltd; 25% Fluoxastrobin SC just reaches first (China) Investment Co., Ltd; 25% pyraclostrobin EC, 50% dimethomorph WP, German BASF AG; 68.75% fluopicolide. propamocarb SC, Bayer Cropscience (China) Co., Ltd.
Embodiment 1: the wetting powder of composite bactericide
Preparation: 25% fluopicolide pyraclostrobin WP(1:4): get the former medicine 5.21kg of 96% fluopicolide, the former medicine 20.84kg of 96% pyraclostrobin, add 2kgM-9,1.5kgCMC, the 5kg neopelex, 65.45kg fine particle calcium carbonate through mix and blend, enters one-level and pulverizes (grain fineness reaches 80 orders); Go again behind the mix and blend, enter secondary and pulverize (grain fineness reaches 325 orders), be packaged as product.
Embodiment 2-6: wetting powder
The preparation method is with embodiment 1, and different is the component that table 1 is listed.
Table 1: embodiment 2-6 component
Embodiment 7-11: wetting powder
The preparation method is with embodiment 1, and different is the component that table 2 is listed.
Table 2: embodiment 7-11 component
Embodiment 1-11 composite bactericide fluopicolide pyraclostrobin WP quality testing
1 active constituent content: utilize the content of liquid chromatography for measuring fluopicolide and pyraclostrobin, the result shows that the content of active ingredient among the embodiment 1-11 fluopicolide pyraclostrobin WP is all greater than 25.00%.
2 wettability determinations: carry out according to GB/T5451-2001 pesticide wettable wettability determination method.The moistening time of embodiment 1-11 fluopicolide pyraclostrobin WP is 55s.
The mensuration of 3 suspensibilities: carry out according to GB/T14825-2006 pesticide wettable suspensibility assay method.The suspensibility of embodiment 1-11 fluopicolide pyraclostrobin WP reaches more than 85%.
The mensuration of 4 fineness: carry out according to the wet screening in the GB/T16150-1995 pesticide wettable fineness assay method.Embodiment 1-11 fluopicolide pyraclostrobin WP by 325 mesh standard sieves (being particle diameter＜44 μ m) reaches 98.5%.
The mensuration of 5 moisture: carry out according to azeotropic distillation method in the GB/T1600-2001 agricultural chemicals moisture assay method.Moisture among the embodiment 1-11 fluopicolide pyraclostrobin WP≤1.8%.
The mensuration of 6 flowabilities: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The result shows that the flow index of embodiment 1-11 fluopicolide pyraclostrobin WP is 1.
The mensuration of 7 foaming characteristics: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The foaming volume of embodiment 1-11 fluopicolide pyraclostrobin WP is 5mL, meets national standard.
The mensuration of 8 thermodynamic stabilities: carry out according to international analysis of agricultural drugs coordination board (CIPAC) method.The resolution ratio of active ingredient≤5% meets national standard after the storage of embodiment 1-11 fluopicolide pyraclostrobin WP heat.
The water dispersible granules of embodiment 12 composite bactericides
Preparation: 25% fluopicolide pyraclostrobin WG(1:4): get 96% fluopicolide 5.21kg, 96% pyraclostrobin 20.84kg adds NNO 2kg; CMC 1.5kg; neopelex 5kg, white carbon 58.45kg is through mix and blend; but air-flow crushing obtains wet-milling; but wet-milling added simultaneously with 2kg cyclodextrin and 5kg water mediate in the kneader, make plastic material, send this material to Squeezinggranulator at last; carry out granulation, obtain the water dispersible granules product by dry, screening.
Embodiment 13-17 water dispersible granules
The preparation method is with embodiment 12, and different is the listed component of table 3.
Table 3: embodiment 13-17 component
Embodiment 18-22: water dispersible granules
The preparation method is with embodiment 12, and different is the listed component of table 4.
Table 4: embodiment 18-22 component
Embodiment 13-22 composite bactericide fluopicolide pyraclostrobin WG quality testing
1, active constituent content measuring
Utilize the content of liquid chromatography for measuring fluopicolide and pyraclostrobin.The result shows, among the embodiment 13-22 among the fluopicolide pyraclostrobin WG content of active ingredient all greater than 25.00%.
2, determination of moisture
Carry out according to azeotropic distillation method in the GB/T1600-2001 agricultural chemicals moisture assay method.The result shows, the moisture among the embodiment 13-22 fluopicolide pyraclostrobin WG≤1.8%.
3, dispersed mensuration
Adopt the dispersiveness of long tube test method determination embodiment 13-22 fluopicolide pyraclostrobin WG, the result is 0.1mL for the sediments of pipe bottom.
4, wettability determination
Adopt the measuring graduates test method determination.The result shows, the wetability of embodiment 13-22 fluopicolide pyraclostrobin WG is good.
5, disintegrative is measured
In the 100mL tool plug graduated cylinder (interior high 22.5cm, internal diameter 28cm) that contains 90mL distilled water, under 25 ℃, add sample particles (0.5g, 250 ~ 1410 μ m), clamp afterwards the middle part of graduated cylinder, clog bung, with the speed of 8r/min around central rotation, until sample fully disintegration in water.The result shows, the disintegration time of embodiment 13-22 fluopicolide pyraclostrobin WG is 2min.
6, suspension stability
Measure according to the GB/T14825-2006 method.The result is that the suspensibility of embodiment 13-22 fluopicolide pyraclostrobin WG reaches more than 90%.
7, the mensuration of thermodynamic stability
Sample is enclosed in the ground wide-mouth bottle, in (50 ± 1) ℃ insulating box, after 4 weeks of storage, analyzed embodiment 13-22 fluopicolide pyraclostrobin WG active constituent content.The result shows that the active ingredient resolution ratio is less than 5%.
Embodiment 23 aqueous emulsions
Preparation: 25% fluopicolide pyraclostrobin aqueous emulsion (1:4): get 96% fluopicolide 5.21kg, 96% pyraclostrobin 20.84kg, add xanthans 1.2kg, NNO 0.8kg, CMC 1.5kg, neopelex 5kg, through mix and blend, under high-speed stirred, water 58.45kg is joined in the oil phase, form the aqueous emulsion of favorable dispersibility.Quality testing meets relevant criterion.
Embodiment 24 suspending agents
25% fluopicolide pyraclostrobin suspending agent (1:4): get 96% fluopicolide 5.21kg, 96% pyraclostrobin 20.84kg, add NNO 2kg, CMC 1.5kg, neopelex 5kg, water 58.45kg, take water as medium, active component, auxiliary agent added in the sand milling still to mix with abrasive media grind, obtain the homogeneous suspension liquid body, liquid is separated making suspending agent with abrasive media.Quality testing meets relevant criterion.
Experimental example 1: different proportioning composite bactericides are to the virulence test of bacterium of downy mildew of cucumber
1.1 for the examination material
1.1.1 for trying bacterial classification: bacterium of downy mildew of cucumber (Pseudoperonospora cubensis), pick up from Dingzhou City in Hebei Province Zhao Cun township Xi Gandecun.
1.1.2 for trying cucumber variety: the kind " the close thorn in Xintai City " of high sense downy mildew.
1.1.3 supply the reagent product: the wetting powder of embodiment 1-11, fluopicolide and pyraclostrobin weight proportion are 1:1 ~ 7, prepare equally the test medicine of fluopicolide and pyraclostrobin weight proportion 9 ~ 3:8 ~ 90 with former medicine, carry out virulence test.
1.2 test method
1.2.1 plant spraying-Ye dish method mensuration bactericide and mixture are to the virulence of bacterium of downy mildew of cucumber
In liquid and deionized water, add 0.1% POLYSORBATE 80.Select the potted plant cucumber plant of 5 ~ 6 true leaves, with laryngeal spray liquid medicine jet is extremely run off to plant.Contrast sprays clear water.Each mass concentration is sprayed 3 strains.After blade dries, process 3 strain plant from each and cut the consistent blade of growing way, break into the leaf dish of diameter 1.5cm with card punch.The leaf dish back side upwards is placed in the culture dish that spreads wet filter paper 15 leaf dishes of each culture dish.Connect the sporangia suspension of 1 10 μ L at each leaf disk center's point, place 18 ~ 20 ℃, the illumination box of 16h illumination/d to cultivate 7 ~ 10d, wait to contrast the abundant morbidity " Invest, Then Investigate " state of an illness.The percentage that accounts for whole leaf disc area according to product spore area is divided sick level: 0 grade, anosis; 1 grade, 1%～5%; 3 grades, 6%～10%; 5 grades, 11%～25%; 7 grades, 26%～50%; 9 grades,＞50%.According to following formula calculate disease index with to Fang Xiao ﹕
Disease index=[∑ (sick leaf dish numbers at different levels * corresponding sick progression)/investigation total leaf dish number * the highest morbidity progression] * 100
Relative control effect (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100
Carry out the probit value of relative control effect and the linear regression analysis between the concentration logarithm value, obtain fluopicolide, pyraclostrobin and composition thereof and suppress the virulence regression equation that bacterium of downy mildew of cucumber infects cucumber leaves, obtain accordingly fluopicolide, pyraclostrobin and composition thereof to bacterium of downy mildew of cucumber EC
90Value and correlation coefficient.
1.2.2 the virulence synergy of fungicide compounding
According to Wadley(1967) formula calculates the not mixed coefficient of synergism of with medicament:
SR=EC
90(th)/EC
90(ob)
A, b represent fluopicolide (A), two kinds of bactericide of pyraclostrobin (B) shared ratio in mixture in the following formula; EC
90(A) and EC
90(B) represent respectively the actual observation EC of A and B
90Value, EC
90(th) expression A, two kinds of bactericide of B are pressed the mixed theoretical EC of a ﹕ b
90Value, EC
90(ob) be that A, two kinds of bactericide of B are pressed the mixed actual observation EC of a ﹕ b
90Value.SR〉1.5, synergy; SR＜0.5, antagonism; SR represents addition between 0.5 and 1.5.
2. result of the test
As known from Table 1, fluopicolide suppresses bacterium of downy mildew of cucumber to infect the effect of cucumber leaves best in candidate's bactericide, EC
50Value is 0.105 μ g/mL.Pyraclostrobin suppresses bacterium of downy mildew of cucumber to infect the effect of cucumber leaves best in the methoxy acrylic bactericide, EC
50Value is 0.377 μ g/mL, secondly is azoles amine bacterium ester, Enestroburin, Fluoxastrobin, azoles bacterium ester and fluorine bacterium mite ester.Therefore, selected fluopicolide is mixed with pyraclostrobin, adopts leaf dish floating method mensuration fluopicolide and the pyraclostrobin rear virulence to bacterium of downy mildew of cucumber that is mixed.
Table 5 plant spraying-Ye dish method is measured bactericide to the virulence of cucumber downy mildew
| Chemicals treatment | Virulence regression equation | EC 50Value (μ g/mL) | Correlation coefficient (r) |
| Fluopicolide | y=0.814x+5.797 | 0.105 | 0.9888 |
| Pyraclostrobin | y=1.157x+5.491 | 0.377 | 0.9995 |
| Azoles amine bacterium ester | y=0.869x+5.253 | 0.512 | 0.9957 |
| Enestroburin | y=0.781x+4.794 | 1.830 | 0.9805 |
| Fluoxastrobin | y=1.941x+3.817 | 4.070 | 0.9894 |
| Azoles bacterium ester | y=0.881x+4.450 | 4.210 | 0.9974 |
| Fluorine bacterium mite ester | y=1.803x+3.245 | 9.410 | 0.9507 |
As known from Table 5, fluopicolide and pyraclostrobin are mixed and suppress synergy proportioning better effects if in 5～1:1～7 scopes that bacterium of downy mildew of cucumber infects cucumber leaves.
Table 6, fluopicolide and pyraclostrobin are mixed to the virulence synergy of bacterium of downy mildew of cucumber
Wetting powder (WP) mode of action of experimental example 2: embodiment 1 composite bactericide is measured 1 different bactericide to the mode of action test of cucumber downy mildew
The susceptible cucumber of plantation in the flowerpot is selected 4 ~ 6 consistent leaf phase cucumber seedlings of growth in the greenhouse, test medicine is mixed with suitable concentration tests, and every processing 5 basins repeat 4 times.1.1 protective effect: the liquid that sprays series concentration is in the blade positive and negative of potted plant cucumber plant to running off, and blank spray clear water treats that liquid dries, place phytotron to cultivate 1,5,10 day after, be 1 * 10 with concentration
5The bacterium of downy mildew of cucumber sporangia suspension inoculation cucumber leaves back side of sporangium/mL, place 20 ℃ of phytotrons, moisturizing was cultivated 7 ~ 10 days under the illumination 16h/d condition, and the state of an illness is respectively processed in investigation when being suitable for adding up to blank blade occurring degree, calculates disease index and relative control efficiency.The percentage that accounts for whole blade area according to lesion area is divided sick level.Fisher ' s least significant difference (LSD) is measured and is carried out statistical analysis in the data SPSS13.0 software.
Sick leaf rate (%)=(the sick number of sheets/investigate total number of sheets) * 100
1.2 therapeutic action: using spray-on process, is 1 * 10 with concentration
5The bacterium of downy mildew of cucumber sporangia suspension of sporangium/mL is inoculated into cucumber plant vacuum side of blade in the flowerpot, place under 20 ℃ of conditions of incubator moisturizing to cultivate 1 day, after the bacterium of downy mildew of cucumber field planting, spray the series concentration liquid in the blade positive and negative to running off, to spray clear water as blank, place 20 ℃ of incubators, continue to be cultured to 6 ~ 8 days among the illumination 16h/d.The state of an illness is respectively processed in investigation when blank blade occurring degree is suitable for adding up, and disease index calculates the same with relative control efficiency.Fisher ' s least significant difference (LSD) is measured and is carried out statistical analysis in the data SPSS13.0 software.
2 result of the tests
From table 5 and table 6 as can be known, 25% fluopicolide pyraclostrobin (1:4 ratio) WP1000～2000 a times liquid protectiveness sprays, and the lasting period reached more than 10 days.Spray 1000 times of liquid of 25% fluopicolide pyraclostrobin WP the prevention of cucumber downy mildew and therapeutic action all significantly are better than 1500 times of liquid of contrast medicament 25% pyraclostrobin EC, 50% dimethomorph WP1200 times liquid and 68.75% fluopicolide. prevention and the therapeutic action of 1000 times of liquid of propamocarb SC.Spraying the therapeutic action of 1500 times of liquid of 25% fluopicolide pyraclostrobin WP and spray 68.75% fluopicolide. the result for the treatment of of 1000 times of liquid of propamocarb SC is suitable, is significantly higher than the result for the treatment of of 1500 times of liquid of 25% pyraclostrobin EC, 1200 times of liquid of 50% dimethomorph WP.
Table 7 sprays 25% fluopicolide pyraclostrobin WP to the prevention effect of cucumber downy mildew
Annotate: analyze according to LSD, in the table after the data identical lowercase be illustrated under the level of P=0.05 difference not significantly (lower with).
Table 825% fluopicolide pyraclostrobin WP therapeutic sprays the therapeutic action to cucumber downy mildew
The effect of experimental example 3: embodiment 1 composite bactericide wetting powder (WP) control fruits and vegetables oomycetes disease
The 1.25% fluopicolide pyraclostrobin WP control cucumber downy mildew test of pesticide effectiveness
" bactericidal agent for preventing and treating cucumber downy mildew test of pesticide effectiveness criterion " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Susceptible variety is cucumber variety " capital is green ".The experimental field is positioned at Dingzhou City Zhao Cun township, Hebei province Xi Gandecun, breed selection, field planting on November 2 in 2010, line-spacing 1m, spacing in the rows 0.3m.
25% fluopicolide pyraclostrobin WP dispenser dosage is 112.5g a.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The contrast medicament: 25% pyraclostrobin EC, dispenser dosage are 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP, dispenser dosage are 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC, dispenser dosage is 618.8g a.i./hm
2(1000 times of liquid), blank spray clear water.Every processing 4 times repeats, totally 28 residential quarters, and randomized arrangement, the residential quarter area is 18m approximately
2
Begin to spray medicine and share medicine 3 times before the downy mildew natural occurrence, be 7 days interval.Spraying time is respectively on December 5th, 2010, December 12, December 19.Adopt Jacto-HD400 type manual sprayer evenly to spray medicine.Be 900L/hm with amount of liquid medicine
2
Investigation morbidity in 7 days (on December 26th, 2010) after last 1 dispenser.Random 4 sampling surveys in every residential quarter, every some investigation 2 strains, whole blades are investigated in every strain, and the percentage that accounts for whole blade area by lesion area on each blade is divided sick level, and Fisher ' s least significant difference (LSD) is measured and carried out statistical analysis in the data SPSS13.0 software.
As known from Table 5,25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm
2Foliage-spray has good control efficiency (85.6%～95.3%) to cucumber downy mildew.Spray 25% fluopicolide pyraclostrobin WP 225g a.i./hm
2Preventive effect be 95.3%, be significantly higher than and spray 25% pyraclostrobin EC 150g a.i./hm
2, 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency.Spray 25% fluopicolide pyraclostrobin WP 150g a.i./hm
2Preventive effect be 90.2%, and spray 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Preventive effect suitable, be significantly higher than and spray 25% pyraclostrobin EC 150g a.i./hm
2Preventive effect.Spray 25% fluopicolide pyraclostrobin WP 112.5g a.i./hm
2Preventive effect with spray 25% pyraclostrobin EC 150g a.i./hm
2Preventive effect suitable.Show before cucumber downy mildew occurs or adopt 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm when just occuring
2Foliage-spray has preferably control effect to cucumber downy mildew.
Table 925% fluopicolide pyraclostrobin WP is to the control efficiency of cucumber downy mildew
2. embodiment 125% fluopicolide pyraclostrobin WP prevents and treats the potato late blight test
Test according to " bactericidal agent for preventing and treating potato late blight test of pesticide effectiveness criterion " in the medicine inspecting institute of the Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Local susceptible variety is Potato Cultivars " Favorita ".The experimental field is positioned at Shizigou township, Chongli County, Hebei province seed farm, plantation on April 29th, 2010, and line-spacing 0.6m, spacing in the rows 0.3m, potato late blight occurs over the years.
25% fluopicolide pyraclostrobin WP dispenser dosage is 112.5g a.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The contrast medicament: 25% pyraclostrobin EC, dispenser dosage are 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP, dispenser dosage are 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC, dispenser dosage is 618.8g a.i./hm
2(1000 times of liquid), blank spray clear water.Each is processed 4 times and repeats, and randomized arrangement is adopted in totally 28 residential quarters.Residential quarter area 20m
2, establish guard row.
Before field natural occurrence, begin to spray medicine, share medicine 4 times, 7 ~ 10 days intervals.Spraying time is respectively July 16, July 23, July 30, August 7.Fine during dispenser.Adopt Jacto-HD400 type manual sprayer spray medicine, reach evenly thoughtful during spraying.Be 900L/hm with amount of liquid medicine
2Duration of test spits 2 times, moderate rain 2 times, and temperature 14-29 ℃, duration of test waters 1 time.Whole duration of test affects without harsh weather.
Investigation in 7 days (on August 14th, 2010) incidence after the last dispenser.5 sampling surveys of every residential quarter diagonal, every some investigation 2 strains, whole compound leaves are investigated in every strain, and the percentage that accounts for whole compound leaf area by lesion area on each compound leaf is divided sick level.Fragmentary morbidity before for the first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.
As known from Table 10,25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm
2Foliage-spray has good control efficiency (83.2%～93.2%) to potato late blight.Spray 25% fluopicolide pyraclostrobin WP 225g a.i./hm
2Preventive effect be 93.2%, be significantly higher than and spray 25% pyraclostrobin EC 150g a.i./hm
2, 50% dimethomorph WP 375ga.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency.Spray 25% fluopicolide pyraclostrobin WP 150g a.i./hm
2Preventive effect with spray 25% pyraclostrobin EC 150g a.i./hm
2, 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency suitable.Show at the beginning of premorbid or the morbidity, adopt 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225ga.i./hm
2Carry out foliage-spray potato late blight is had preferably control efficiency.
Table 1025% fluopicolide pyraclostrobin WP is to the control efficiency of potato late blight
3.25% fluopicolide pyraclostrobin WP prevents and treats the downy mildew of garpe test of pesticide effectiveness
" bactericidal agent for preventing and treating downy mildew of garpe test of pesticide effectiveness criterion " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Susceptible variety is grape variety " rose fragrant ".Experimental field be positioned at Jing Tangpu village, river for transporting grain to the capital informer times town, Xushui County, Hebei province, the age of tree 5 years.
Test medicine: 25% fluopicolide pyraclostrobin WP, dispenser dosage are 1000 times of liquid, 1500 times of liquid, 2000 times of liquid.Contrast medicament: 1500 times of liquid of 25% pyraclostrobin EC, 1200 times of liquid of 50% dimethomorph WP, SC1000 times of liquid of 68.75% fluopicolide propamocarb, blank spray clear water.Every processing 4 times repeats, and per 5 strain grapes are 1 residential quarter, totally 28 residential quarters, randomized arrangement.
When downy mildew is sporadicly fallen ill on the grape leave (on August 1st, 2010) begin to spray medicine, dispenser is 3 times altogether, 7 ~ 10 days intervals.Adopt Jacto-HD400 type manual sprayer evenly to spray medicine, running off is degree.
Investigation morbidity in 7 days after last 1 dispenser.10 of 5 upper upper blade of branch of every strain investigation, the percentage that accounts for whole blade area by lesion area on each blade is divided sick level.
As known from Table 11,25% fluopicolide pyraclostrobin WP1000 among the embodiment 1 ~ 2000 times of liquid foliage-sprays have preferably control efficiency (86.9% ~ 92.1%) to downy mildew of garpe.The preventive effect that sprays 25% fluopicolide pyraclostrobin WP1000 ~ 1500 times of liquid among the embodiment 1 is significantly higher than the control efficiency that sprays 1500 times of liquid of 25% pyraclostrobin EC and 1200 times of liquid of 50% dimethomorph WP, and is suitable with the preventive effect of 1000 times of liquid of 68.75% fluopicolide propamocarb SC.The preventive effect that sprays WP2000 times of liquid of 25% fluopicolide pyraclostrobin is suitable with the control efficiency that sprays 1500 times of liquid of 25% pyraclostrobin EC and 1200 times of liquid of 50% dimethomorph WP.Result of the test shows, adopts 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225ga.i./hm before downy mildew of garpe occurs or when just occuring
2Foliage-spray has preferably control efficiency to downy mildew of garpe.
25% fluopicolide pyraclostrobin WP among table 11 embodiment 1 is to the control efficiency of downy mildew of garpe
4. 25% fluopicolide pyraclostrobin WP among the embodiment 1 prevents and treats the tomato late blight test of pesticide effectiveness
Susceptible variety is tomato variety " Borrow's tower ".The experimental field is positioned at Xin Xing village, Dingzhou City Yang Jiazhuan township, Hebei province, is the breed selection tomato, line-spacing 0.8m, spacing in the rows 0.3m.
Dispenser dosage is 112.5g a.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The contrast medicament: 25% pyraclostrobin EC, dispenser dosage are 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP, dispenser dosage are 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC, dispenser dosage is 618.8g a.i./hm
2(1000 times of liquid), blank spray clear water.Each is processed 4 times and repeats, and randomized arrangement is adopted, residential quarter area 15m in totally 28 residential quarters
2
Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on April 8th, 2010, April 15, April 22.Adopt Jacto-HD400 type manual sprayer evenly to spray medicine.Be 900L/hm with amount of liquid medicine
2
Investigation in 7 days (on April 29th, 2010) incidence after last 1 dispenser.Random 5 sampling surveys in every residential quarter, every some investigation 2 strains, whole compound leaves are investigated in every strain, and the percentage that accounts for whole compound leaf area by lesion area on each compound leaf is divided sick level.
As known from Table 12,25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm
2Foliage-spray has good control efficiency (84.1%～93.5%) to tomato late blight.Spray 25% fluopicolide pyraclostrobin WP 225g a.i./hm
2Preventive effect be significantly higher than and spray 25% pyraclostrobin EC 150g a.i./hm
2With 50% dimethomorph WP 375g a.i./hm
2Control efficiency, and spray 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency suitable.Spray 25% fluopicolide pyraclostrobin WP 150g a.i./hm
2Preventive effect be 88.7%, and spray 25% pyraclostrobin EC 150g a.i./hm
2, 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8ga.i./hm
2Control efficiency suitable.The result shows, adopts 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm before tomato late blight occurs or when just occuring
2Foliage-spray has preferably control efficiency to tomato late blight.
Table 1225% fluopicolide pyraclostrobin WP is to the control efficiency of tomato late blight
The 5.25% fluopicolide pyraclostrobin WP control Chinese cabbage downy mildew test of pesticide effectiveness
Susceptible variety is Chinese cabbage cultivar " Ji grinds No. 2 ".The experimental field is positioned at Yang Zhuan township, Baoding, Hebei province rear batallion village, open-air cultivation, line-spacing 0.5m, spacing in the rows 0.3m.
25% fluopicolide pyraclostrobin WP dispenser dosage is 112.5g a.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The contrast medicament: 25% pyraclostrobin EC, dispenser dosage are 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP, dispenser dosage are 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC, dispenser dosage is 618.8g a.i./hm
2(1000 times of liquid), blank spray clear water.Each is processed 4 times and repeats, and randomized arrangement is adopted, residential quarter area 20m in totally 28 residential quarters
2
Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on August 17th, 2010, August 25, September 2.Adopt evenly thoughtful spray medicine of Jacto-HD400 type manual sprayer.Be 900L/hm with amount of liquid medicine
2
Investigation in 7 days (on September 9th, 2010) incidence after last 1 dispenser.Random 5 sampling surveys in every residential quarter, every some investigation 2 strains, whole blades are investigated in every strain, and the percentage that accounts for whole blade area by lesion area on each blade is divided sick level.
As known from Table 13,25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm
2Foliage-spray has good control efficiency (87.9%～95.2%) to Chinese cabbage downy mildew.25% pyraclostrobin EC 150g a.i./hm
2, 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency.Spray 25% fluopicolide pyraclostrobin WP 150g a.i./hm
2Preventive effect with spray 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8ga.i./hm
2Control efficiency suitable.Spray 25% fluopicolide pyraclostrobin WP 112.5ga.i./hm
2Preventive effect with spray 25% pyraclostrobin EC 150g a.i./hm
2Control efficiency suitable.Show before Chinese cabbage downy mildew occurs or adopt 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm when just occuring
2Foliage-spray has preferably control efficiency to Chinese cabbage downy mildew.
Table 1325% fluopicolide pyraclostrobin WP is to the control efficiency of Chinese cabbage downy mildew
The 6.25% fluopicolide pyraclostrobin WP control capsicum epidemic disease test of pesticide effectiveness
" bactericidal agent for preventing and treating capsicum epidemic disease test of pesticide effectiveness criterion " carries out in the medicine inspecting institute of the experimental evidence Ministry of Agriculture " pesticide field efficacy medicine test criterion ".Susceptible variety is capsicum variety " the ears of an ox or cow king ".The experimental field is positioned at the solid cities and towns Bei Zhuantoucun in Dingxing County, Hebei province, is the breed selection capsicum, line-spacing 0.8m, spacing in the rows 0.3m.。
Test medicine: 25% fluopicolide pyraclostrobin WP, dispenser dosage is 112.5ga.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The contrast medicament: 25% pyraclostrobin EC, dispenser dosage are 150g a.i./hm
2(1500 times of liquid); 50% dimethomorph WP, dispenser dosage are 375g a.i./hm
2(1200 times of liquid); 68.75% fluopicolide propamocarb SC, dispenser dosage is 618.8ga.i./hm
2(1000 times of liquid), blank spray clear water.Each is processed 4 times and repeats, and randomized arrangement is adopted, residential quarter area 20m in totally 28 residential quarters
2
Premorbid begins to spray medicine, shares medicine 3 times, and be 7 days interval.Spraying time is respectively on May 9th, 2010, May 16, May 23.Adopt evenly thoughtful spray medicine of Jacto-HD400 type manual sprayer.Be 900L/hm with amount of liquid medicine
2
Investigate 7 days (on May 30th, 2010) after last 1 dispenser.Random 5 sampling surveys in every residential quarter, every some investigation 10 strains.Stage division: 0 grade: anosis; 1 grade: rhizome section is without scab, and indivedual branches or fruit are fallen ill; 3 grades: the slight morbidity of rhizome section or the following branch of half or fruit morbidity; 5 grades: the moderate morbidity of rhizome section or branch more than half or fruit morbidity; 7 grades: rhizome section seriously falls ill, and large of plant is wilting; 9 grades: the whole wilting death of plant.Fragmentary morbidity before for the first time dispenser, state of an illness radix is designated as zero.Respectively process the state of an illness according to above stage division record, calculate the incidence of disease, disease index, preventive effect.
As known from Table 14,25% fluopicolide pyraclostrobin WP, 112.5 ~ 225g a.i./hm
2Foliage-spray has good control efficiency (84.2%～91.6%) to capsicum epidemic disease.Spray 25% fluopicolide pyraclostrobin WP 225g a.i./hm
2Preventive effect be significantly higher than and spray 25% pyraclostrobin EC 150g a.i./hm
2With 50% dimethomorph WP 375g a.i./hm
2Control efficiency.Spray embodiment 1 fluopicolide pyraclostrobin WP 150g a.i./hm
2Preventive effect with spray 50% dimethomorph WP 375g a.i./hm
2With 68.75% fluopicolide propamocarb SC 618.8g a.i./hm
2Control efficiency suitable.The result shows, before capsicum epidemic disease occurs or when just occuring, adopts 25% fluopicolide pyraclostrobin WP, 112.5 ~ 225ga.i./hm
2Foliage-spray has preferably control efficiency to capsicum epidemic disease.
Table 1425% fluopicolide pyraclostrobin WP is to the control efficiency of capsicum epidemic disease
Pot experiment shows that 25% fluopicolide pyraclostrobin WP1000 among the embodiment 1～2000 times of foliar sprays have preferably preventive effect and therapeutic action to cucumber downy mildew, and the lasting period is longer.Field trial shows 25% fluopicolide pyraclostrobin WP, 112.5～225g a.i./hm
2Or 1000 ~ 2000 times of dilution foliar sprays all have good preventive and therapeutic effect to cucumber downy mildew, potato late blight, downy mildew of garpe, tomato late blight, Chinese cabbage downy mildew and capsicum epidemic disease, are better than conventional medicine 25% pyraclostrobin EC, 50% dimethomorph WP and 68.75% fluopicolide propamocarb SC.According to observations, embodiment 1 fluopicolide pyraclostrobin WP 112.5～225g a.i./hm
2Or 1000 ~ 2000 times of liquid foliar sprays have no adverse effects to cucumber, potato, grape, tomato, Chinese cabbage and capsicum and other beneficial organism.Can use 25% fluopicolide pyraclostrobin WP, 112.5～225g a.i./hm in the production
2Or 1000 ~ 2000 times of liquid foliar spray control fruits and vegetables oomycetes diseases.
Experimental example 4: the composite bactericide fluopicolide pyraclostrobin WP control fruits and vegetables oomycetes among the embodiment 2-11 is sick
Susceptible variety is Chinese cabbage cultivar " Ji grinds No. 2 ".The experimental field is positioned at Yang Zhuan township, Baoding, Hebei province rear batallion village, open-air cultivation, line-spacing 0.5m, spacing in the rows 0.3m.
Dispenser dosage is 112.5g a.i./hm
2(2000 times of liquid), 150g a.i./hm
2(1500 times of liquid), 225g a.i./hm
2(1000 times of liquid).The result shows, the fluopicolide pyraclostrobin WP 112.5 ~ 225g a.i./hm among the embodiment 2-11
2Foliage-spray has good control efficiency (87.9%～95.2%) to Chinese cabbage downy mildew.
Fluopicolide pyraclostrobin WP among the table 15 embodiment 2-11 is to the control efficiency of Chinese cabbage downy mildew
The control efficiency of the composite bactericide fluopicolide pyraclostrobin WG among the same test example 12-22, embodiment 23 composite bactericide fluopicolide pyraclostrobin aqueous emulsions, embodiment 24 composite bactericide fluopicolide pyraclostrobin suspending agents, the results list 16.
Composite bactericide fluopicolide pyraclostrobin among the table 16 embodiment 12-24 is to the control efficiency of Chinese cabbage downy mildew
Claims (10)
1. the composite bactericide of a containing fluopicolide, it is characterized in that: be comprised of active component and auxiliary agent, described active component is comprised of fluopicolide and methoxy acrylic bactericide, and its weight ratio is 1～10:1～90.
2. composite bactericide as claimed in claim 1, it is characterized in that: the weight ratio of described fluopicolide and methoxy acrylic bactericide is 1～7:1～20.
3. composite bactericide as claimed in claim 2, it is characterized in that: the weight ratio of described fluopicolide and methoxy acrylic bactericide is 1 ~ 5:1～7.
4. such as the arbitrary described composite bactericide of claim 1 ~ 3, it is characterized in that: described methoxy acrylic bactericide is one or more the mixing in pyraclostrobin, Fluoxastrobin, azoles amine bacterium ester, Enestroburin, azoles bacterium ester, the fluorine bacterium mite ester.
5. the composite bactericide of containing fluopicolide as described in arbitrary such as claim 1 ~ 3, it is characterized in that: the formulation of described composite bactericide is soluble powder, soluble granula, water dispersant, aqua, wetting powder, soluble liquid, missible oil, aqueous emulsion, microemulsion or suspending agent.
6. the composite bactericide of containing fluopicolide as described in arbitrary such as claim 1 ~ 3, it is characterized in that: described auxiliary agent comprises wetting agent, dispersant, filler, water, binding agent, thickener, anti-foaming agent and disintegrant, and described composite bactericide comprises the component of following weight portion:
7. composite bactericide as claimed in claim 6 is characterized in that: described dispersant is one or more in the mixture of lignin sulfonic acid sodium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, 4-oleoyl-methoxy benzenesulfonic acid sodium salt, sanlose, alkylphenol polyoxyethylene, ethylene glycol monobutyl ether or polyoxy ethyl alkyl ether, polyoxyethylene nonylphenol ether and neopelex;
Described wetting agent is a kind of in pelopon A, fatty alcohol-polyoxyethylene ether or the alkylphenol-polyethenoxy base formaldehyde condensation products sulfonate;
Described filler is one or more in white carbon, kaolin, fine particle calcium carbonate, diatomite or the bentonite;
Described binding agent is one or more in cyclodextrin, carboxymethyl cellulose, gum Arabic, the polyvinyl alcohol.
8. composite bactericide as claimed in claim 6, it is characterized in that: described thickener is one or more in xanthans, CMC, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol;
Described anti-foaming agent is silicone anti-foaming agent M10 or anti-foaming agent C;
Described disintegrant is a kind of in bentonite, calcium chloride, anhydrous sodium sulfate, kaolin, diatomite, ammonium sulfate, urea, sodium phosphate trimer or the sodium pyrophosphate;
Described antifreezing agent is one or more the mixture in glycerine, urea, ethylene glycol, the propane diols.
9. the application of the arbitrary described composite bactericide of claim 1～8 in control fruits and vegetables oomycetes disease.
10. application as claimed in claim 9 is characterized in that: described fruits and vegetables oomycetes disease comprises downy mildew, white blister, damping off, root rot, stem rot, base rot disease, fruit rot, cotton disease and the late blight of fruit and/or vegetables.
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| CN103004819A (en) * | 2012-11-30 | 2013-04-03 | 王学权 | Fluopicolide-containing bactericide composition |
| CN103004822A (en) * | 2013-01-21 | 2013-04-03 | 山东滨农科技有限公司 | Bactericide composition containing pyraclostrobin and fluopicolide |
| CN104115853A (en) * | 2013-04-23 | 2014-10-29 | 陕西美邦农药有限公司 | Pesticide composition containing carbamate bactericide |
| CN105158382A (en) * | 2015-06-04 | 2015-12-16 | 上海市农业技术推广服务中心 | Method for detecting fluopicolide in fruits and vegetables |
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| CN112970760A (en) * | 2021-02-24 | 2021-06-18 | 安徽科技学院 | Ternary compound bactericide and application thereof in prevention and treatment of wheat stem basal rot |
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| CN103004822A (en) * | 2013-01-21 | 2013-04-03 | 山东滨农科技有限公司 | Bactericide composition containing pyraclostrobin and fluopicolide |
| CN104115853A (en) * | 2013-04-23 | 2014-10-29 | 陕西美邦农药有限公司 | Pesticide composition containing carbamate bactericide |
| CN105158382A (en) * | 2015-06-04 | 2015-12-16 | 上海市农业技术推广服务中心 | Method for detecting fluopicolide in fruits and vegetables |
| CN106256204A (en) * | 2015-06-17 | 2016-12-28 | 沈阳中化农药化工研发有限公司 | A kind of insecticidal and bactericidal composition |
| CN107125254A (en) * | 2017-05-30 | 2017-09-05 | 兴农药业（中国）有限公司 | A kind of composition pesticide of fluopicolide and pyraclostrobin |
| CN111316995A (en) * | 2018-12-17 | 2020-06-23 | 中化国际(控股)股份有限公司 | Pesticide composition, pesticide preparation and application of pesticide composition and pesticide preparation |
| CN112970760A (en) * | 2021-02-24 | 2021-06-18 | 安徽科技学院 | Ternary compound bactericide and application thereof in prevention and treatment of wheat stem basal rot |
| CN113812413A (en) * | 2021-11-02 | 2021-12-21 | 中国农业科学院农业环境与可持续发展研究所 | Double-loading nano pesticide controlled release agent containing fluopyram and pyraclostrobin and preparation method thereof |
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