CN102715173B - Bactericidal composition containing phenylate diazole and fluoxastrobin and application of bactericidal composition - Google Patents
Bactericidal composition containing phenylate diazole and fluoxastrobin and application of bactericidal composition Download PDFInfo
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- CN102715173B CN102715173B CN2012102239569A CN201210223956A CN102715173B CN 102715173 B CN102715173 B CN 102715173B CN 2012102239569 A CN2012102239569 A CN 2012102239569A CN 201210223956 A CN201210223956 A CN 201210223956A CN 102715173 B CN102715173 B CN 102715173B
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- bactericidal composition
- fluoxastrobin
- phenylate
- disease
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- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
The invention relates to a bactericidal composition and application thereof. The bactericidal composition contains phenylate diazole and fluoxastrobin and is used for controlling potato early blight and later blight, wheat powdery mildew and rust disease, vegetable leaf spot disease and downy mildew. Compared with a single bactericide, the composition has an obvious synergistic effect and is favorable for overcoming and delaying the resistance of bacteria.
Description
[technical field]
The invention belongs to technical field of pesticide.More specifically, the present invention relates to a kind of bactericidal composition that contains the two azoles of phenylate and fluoxastrobin, also relate to the purposes of described bactericidal composition.
[background technology]
Potato late blight (Phytophthora infestans) is to cause a kind of destructive popular disease that potato haulm is dead and stem tuber rots, and by phytophthora infestans, is caused, for just infecting source, it requires the nice and cool weather of high humidity with the disease potato that carries disease germs.Under suitable condition, germ rapidly breeding repeatedly infect again cause popular; Target (Alternaria solani) pathogen is the early epidemic Alternaria, except the harm potato, can also endanger the vegetables such as tomato, capsicum of booth and greenhouse culture.Germ at invalid body with survive the winter on the potato piece in spite of illness, was infected morbidity with conidium or mycelia when next year, potato seed started to germinate.Potato raw is met cloudy weather for several days running weather or humidity for a long time continuously higher than 70%, falls ill seriously even popular, and this disease is in recent years in rising trend.Early blight and late blight are often produced and are caused great loss to potato.
Wheat rust is again jaundice, and 3 kinds of yellow rust, black stem rust and leaf rust are arranged.That on China's Wheat Production, distribution is wide, propagation is fast, the important disease that hazard area is large.Wherein with stripe rust of wheat, occur the most general and serious.After wheat generation rust, in body, nutrient is absorbed, and chlorophyll is destroyed, and a large amount of soruss are broken through wheat leaf, straw epidermis, have a strong impact on yield and quality of wheat; Wheat powdery mildew (Blumeria graminis) is a kind of worldwide disease, in each main wheat producing countries, distribution is arranged all, and China's Along The Coast of Shandong Province, Sichuan, Guizhou, Yunnan occur general, causes harm also heavy.In recent years should disease northeastward, North China, northwest Mai Qu, the gesture that is on the rise is also arranged.This disease can be encroached on each organ of wheat plant overground part, but take blade and leaf sheath as main, when morbidity is heavy, grain husk shell and awns also can be injured.All can cause harm to the strain phase in seedling stage, main harm blade, also can cause harm when serious leaf sheath, stem stalk and fringe section.General face of blade scab is more than reverse side, and lower blade, more than upper blade, can heal when scab is many in flakes.And cause yellow leaf withered.After stem stalk and leaf sheath were injured, plant easily lodged.
The vegetable leaf pinta is that vegetables produce one of upper important disease, and distributed more widely, there is generation various places, generally in spring, two seasons of autumn, occurs heavier.The incidence of disease 10%～60%, serious plot or canopy chamber reach 100%, but the underproduction 10%～30%, but the underproduction more than 60% during especially severe.This disease vegetables such as muskmelon, cucumber, wax gourd, sponge gourd, balsam pear of also can causing harm.The false pseudomonas bacillus of the Pseudomonas of causing a disease.Vegetables downy mildew is the most important disease of class on protection ground vegetables, generally can cause the underproduction 20%～30%, can reach more than 50% when serious, and along with the expansion of protecting Di Peng chamber area, its harm is more and more heavy.
The inventor studies discovery, bactericidal composition take the two azoles of phenylate and fluoxastrobin as active ingredient, be used for target and late blight, the control of wheat rust and powdery mildew, vegetable leaf pinta and downy mildew, can delay the drug-fast generation of germ, improving actual preventive effect, enlarge fungicidal spectrum and reduce drug cost, is the important means of comprehensive control of disease.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of bactericidal composition that contains the two azoles of phenylate and fluoxastrobin.
Another object of the present invention is to provide the purposes of described bactericidal composition.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of bactericidal composition that contains the two azoles of phenylate and fluoxastrobin.This bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
The two azoles of phenylate (English common name: Difenodiconazole), chemical name: 2-[2-chloro-4-(4-chlorobenzene oxygen) phenyl]-1, two (1H-1,2,4-the triazol-1-yl)-2-propyl alcohol of 3-.CN 200610140836.7 discloses chemical structural formula, preparation method and the sterilizing use of the two azoles of phenylate, in present specification, this patent application is incorporated herein by reference in full.
B is fluoxastrobin;
Fluoxastrobin (Fluoxastrobin) is that novel, the interior absorption cauline leaf of wide spectrum Er Qing oxazine class of Bayer Cropscience Co.,Ltd's report processed bactericide.Its application optimum period, is wide, no matter as spore germination early stage at fungal infection, germ tube growth, or at growth period of hypha, can provide extraordinary protection and therapeutic action, especially very effective to spore germination and initial stage dip-dye.Because of the systemic activity of its excellence that has, so it can be rapidly absorbed, and can transmit to top equably in leaf section, therefore have good resistance of rainwater washing against ability.Fluoxastrobin is mainly used in cauline leaf and processes, can effectively prevent and treat nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease in cereal crop, potato, vegetables and coffee etc., such as epidemic disease, rust, Ying's rot, net blotch, powdery mildew, leaf spot or downy mildew etc.Can very effectively prevent and treat target and late blight; Can very effectively prevent and treat vegetable leaf pinta, downy mildew.Can very effectively prevent and treat cereal crop leaf spot, glume blight, leaf rust, yellow rust, moire disease, brown spot, net blotch, powdery mildew is also had good drug effect, and can the double full rot of controlling.
In bactericidal composition of the present invention, the weight ratio of described component A and described B component is 1:100 ~ 100:1.
Preferably, in this bactericidal composition, the weight ratio of component A and B component is 1:50～50:1.
More preferably, in this bactericidal composition, the weight ratio of component A and B component is 1:10～10:1.
The invention still further relates to bactericidal composition of the present invention and preventing and treating target, late blight, wheat rust, powdery mildew, the purposes in vegetable leaf pinta, downy mildew.
The present invention relates to a kind of bactericide.
This bactericide contains 0.1～90 above-mentioned bactericidal composition of % by weight and surplus is acceptable carrier and auxiliary agent on agricultural chemicals.
Preferably, this bactericide contains 10～80 above-mentioned bactericidal compositions of % by weight.
More preferably, this bactericide contains 28～62 above-mentioned bactericidal compositions of % by weight.
Except as otherwise noted, " % " involved in the present invention is " percentage by weight ".
Bactericide involved in the present invention can be mixed with the upper acceptable formulation of agricultural, preferred dosage form is suspending agent, suspension emulsion, aqueous emulsion, microemulsion, wetting powder or water dispersible granules.All these formulations all adopt method as well known to those skilled in the art to prepare by acceptable carrier on above-mentioned bactericidal composition and agricultural chemicals and auxiliary agent.
As for the water-dispersible grain formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of dispersant, wetting agent, disintegrant, binding agent and filler.
Described dispersant is selected from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described disintegrant is selected from ammonium sulfate, urea, sucrose, glucose, citric acid, succinic acid or sodium bicarbonate.
Described binding agent is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family and microcrystalline cellulose class.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, sepiolite, talcum powder, attapulgite or potter's clay etc.
The dispersant that uses when preparing the water-dispersible grain formulation, wetting agent, disintegrant, binding agent and filler are all product solds in the market.
As for the wettable powder formulation, those skilled in the art can adopt conventional preparation method to be obtained by the main auxiliary agent preparation of dispersant, wetting agent and filler.
Described dispersant is selected from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite or potter's clay etc.
The dispersant that uses when preparing the wettable powder formulation, wetting agent and filler are all product solds in the market.
As for suspending agent type, those skilled in the art can adopt conventional preparation method to be obtained by these auxiliary agent preparations of dispersant, wetting agent, thickener, preservative, defoamer and antifreezing agent.
Described dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate or TERSPERSE 2425(U.S. Huntsman Corporation and produces, the alkyl naphthalene sulfonic acid salt).
Described wetting agent is selected from the poly-basic ether formaldehyde condensation products sulphate of alkylphenol-polyethenoxy, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate or TERSPERSE 2500(U.S. Huntsman Corporation and produces).
Described thickener is selected from xanthans, aluminium-magnesium silicate or bentonite.
Described preservative is selected from benzoic acid, Sodium Benzoate or BIT(1, the 2-benzisothiazole-3-ketone).
Described defoamer is silicone based defoamer.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
The dispersant that uses when preparing suspending agent type, wetting agent, thickener, preservative, defoamer and antifreezing agent are all product solds in the market.
As for the suspension emulsion type, those skilled in the art can adopt conventional preparation method to be obtained by these auxiliary agent preparations of dispersant, wetting agent, emulsifier, solvent, thickener, preservative, defoamer and antifreezing agent.
Described dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate or TERSPERSE 2425(U.S. Huntsman Corporation and produces, the alkyl naphthalene sulfonic acid salt).
Described wetting agent is selected from the poly-basic ether formaldehyde condensation products sulphate of alkylphenol-polyethenoxy, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate or TERSPERSE 2500(U.S. Huntsman Corporation and produces).
Described emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agriculture breast 600# phosphate), Nongru-700 #, agriculture breast 2201#, Span-60#, emulsifier T-60, TX-10, agriculture breast 1601#, agriculture breast 600#, agriculture breast 400#;
Described solvent is selected from dimethylbenzene, toluene, cyclohexanone or solvent naphtha (trade mark: S-150, S-180, S-200);
Described thickener is selected from xanthans, aluminium-magnesium silicate or bentonite.
Described preservative is selected from benzoic acid, Sodium Benzoate or BIT(1, the 2-benzisothiazole-3-ketone).
Described defoamer is silicone based defoamer.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
The dispersant that uses when preparing suspending agent type, wetting agent, emulsifier, solvent, thickener, preservative, defoamer and antifreezing agent are all product solds in the market.
As for Water emulsion, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, solvent, stabilizing agent, antifreezing agent, thickener and preservative.
Described emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agriculture breast 600# phosphate), Nongru-700 #, agriculture breast 2201#, Span-60#, emulsifier T-60, TX-10, agriculture breast 1601#, agriculture breast 600# or agriculture breast 400#.
Described solvent is selected from dimethylbenzene, toluene, cyclohexanone or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Described thickener is selected from xanthans, bentonite or aluminium-magnesium silicate.
Described preservative is selected from BIT(1, the 2-benzisothiazole-3-ketone), benzoic acid or Sodium Benzoate.
The emulsifier that uses when preparing Water emulsion, solvent, stabilizing agent, antifreezing agent, thickener and preservative are all product solds in the market.
As for microemulsion dosage, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, cosolvent, solvent and stabilizing agent.
Described emulsifier is selected from calcium dodecyl benzene sulfonate (agriculture breast 500#), Nongru-700 #, agriculture breast 2201#, this dish-60#, Tween-80, TX-10, agriculture breast 1601, agriculture breast 600# or agriculture breast 400#.
Described cosolvent is selected from methyl alcohol, isopropyl alcohol, n-butanol or ethanol.
Described solvent is selected from cyclohexanone, 1-METHYLPYRROLIDONE, dimethylbenzene, toluene or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
The emulsifier that uses when preparing microemulsion dosage, cosolvent, solvent and stabilizing agent are all product solds in the market.
Bactericidal composition of the present invention can provide with the finished product preparation form, and namely in composition, each material mixes; Also can provide with the single dose preparation, directly mix in bucket (tank) before using.Concentrate of the present invention usually and water be mixed to get the concentration of required active substance.
The invention still further relates to a kind of target, late blight of preventing and treating, wheat rust, powdery mildew, the method of vegetable leaf pinta, downy mildew disease, before or after potato, wheat or vegetable crop introduced disease, use above-mentioned bactericidal composition of the present invention or bactericide to the place of its growth or storage.Its application process is the conventional application process of agricultural chemicals, such as pouring, injection, spraying, dusting, broadcast sowing etc.
[beneficial effect]
The present invention has following beneficial effect:
(1) compare with single dose, bactericidal composition of the present invention has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, reduce drug cost, environmental contamination reduction and residue of pesticide;
(3) composition is comprised of the active ingredient of different mechanism of action, and action site increases, and is conducive to overcome and delays the drug-fast generation of germ.
[embodiment]
Can understand better the present invention by following embodiment.If no special instructions, active ingredient ratio of the present invention is weight ratio.
Embodiment 1
One, biological activity determination
Embodiment 1: the composite Toxicity Determination to potato morning, late blight of the two azoles of phenylate and fluoxastrobin
Subjects target (Alternaria solani) and potato late blight (Phyophthora infestans).
Adopt the mycelial growth rate method to measure the two azoles of phenylate and the former medicine of fluoxastrobin and the composite inhibitory action to potato morning, late disease bacteria of the two azoles proportionings different from fluoxastrobin of phenylate.With tested bacterial strain after cultivating 6d on the PDA flat board, buy from colony edge the bacterium cake that the cut-off footpath is 5mm, the bacterium cake is received on the flat board that contains different quality concentration medicament, contrast adds sterile water, every processing repeats 3 times, be placed in 25 ℃, every day the 12h dark and the condition of 12h illumination under to cultivate the 6d(late blight be insulating box dark culturing 8～10d 18 ℃ of temperature, wait contrasting colony diameter to be at least 50mm above but while not covering with whole culture dish), adopt the measurement of right-angled intersection method respectively to process colony diameter.Calculate and respectively process bacterium colony growth diameter and inhibiting rate, the data DPS software carries out statistical analysis.
Take the mass concentration logarithm set as abscissa, inhibiting rate probability value is ordinate, obtains each medicament to the virulence regression equation for the examination germ, correlation coefficient r and concentration EC in effectively suppressing
50Value.
The co-toxicity coefficient computational methods: according to the abundant method of Sun Yun (document ... ... each EC that processes that ..) will measure
50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, obtain theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
The content (%) of toxicity index * A in mixed of theoretical mixed toxicity index (TTI)=A
The content (%) of toxicity index * B of+B in mixed
If co-toxicity coefficient, greater than 120, shows the two azoles of phenylate and fluoxastrobin mixture, synergistic effect is arranged; , if co-toxicity coefficient is starkly lower than 100, show that its mixture is antagonism; , if co-toxicity coefficient is 100～120, show that its mixture is summation action.
The two azoles of described phenylate and fluoxastrobin composite to potato early, the Toxicity Determination of late blight lists in table 1 and table 2.
Table 1: the two azoles of phenylate and the composite result of Toxicity Determination to target of fluoxastrobin
Table 2: the two azoles of phenylate and the composite result of Toxicity Determination to potato late blight of fluoxastrobin
Embodiment 2: the two azoles of phenylate and the composite Toxicity Determination to wheat powdery mildew, rust of fluoxastrobin
Powdery mildew test target wheat powdery mildew (Erysiphe graminis) is cultivated and is selected susceptible variety (raising wheat No. 6) potted plant by the long-term live body of this laboratory, treat that seedling grows to 2 leaves～3 leaf phases, naturally, dries after spraying is processed, the fresh spore of Powdery Mildew that the wheat leaf blade of falling ill was produced in upper 24 hour is evenly shaken off to be inoculated on 2～3 potted plant wheat seedlings of leaf phase that chemicals treatment crosses.Postvaccinal examination material moves to 20 ℃, relative moisture 80% left and right controllable greenhouse glass room is interior cultivates.Note keeping basin soil moistening every day, investigate according to the classification of blank incidence about 7 days.With reference to pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating Cereal powdery mildew.
The disease scale standard is as follows:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 16% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are with example 1.
The results are shown in Table 3 for the two azoles of phenylate and the composite Toxicity Determination to wheat powdery mildew of fluoxastrobin.
Table 3: the two azoles of phenylate and the composite result of Toxicity Determination to wheat powdery mildew of fluoxastrobin
The rust experiment target is designated as wheat leaf rust pathogen (Puccinia recondita), and wheat breed is for raising wheat No. 6, and every basin is selected approximately l0 strain wheat seeding, the 3-4 leaf phase to be grown to is standby, reagent agent and proportioning medicament are mixed with the liquid that presets concentration, and 4 repetitions are established in the processing of spraying, every processing.Get the wheat leaf blade that covers with the leaf rust bacterium after 24h, with sterile water, wash gently and get surperficial fresh spore, with double gauze, filter, making spore concentration is the suspension of 100,000/ml left and right, spray inoculation (pressure 0.1MPa).After connecing bacterium wheat seeding is under 20 ℃ of conditions, moisturizing one day, then put back to greenhouse, and when contrasting abundant morbidity, by field control effectiveness test criterion grade scale, carry out state of an illness investigation after the week, refer to calculate preventive effect by disease.Obtain EC with return law of the straight line
50
The disease scale standard is as follows:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 25% of one-piece blade area;
5 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
7 grades: lesion area accounts for 51% ~ 75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are with example 1.
The results are shown in Table 4 for the two azoles of phenylate and the composite Toxicity Determination to wheat rust of fluoxastrobin.
Table 4: the two azoles of phenylate and the composite result of Toxicity Determination to wheat rust of fluoxastrobin
Embodiment 3: the two azoles of phenylate and the composite Toxicity Determination to vegetable leaf pinta, downy mildew of fluoxastrobin
Subjects: the leaf spot target is Chinese cabbage black spot (Alternaria brassicae), Chinese cabbage downy mildew (Peronospora parasitica).
Test method: with reference to " the agricultural industry criteria NY/T 1156.2-2006 of the People's Republic of China (PRC) " mycelial growth rate method.Each medicament is established respectively 5 dosage by active constituent content and is processed, with above-mentioned each pathogen PDA medium culture, when bacterium colony just covers with culture dish, break into the bacterium piece with the card punch of diameter 5mm at colony edge, with transfer needle, the bacterium piece is moved to the toxic PDA medium that is mixed with in advance central, then be placed in 25 ℃ of incubators and cultivate, every processing repeats 4 times.Depending on CK colony growth situation, adopt the right-angled intersection method to measure and respectively process colony diameter cm with slide calliper rule, obtain the correction inhibition percentage.Linear regression analysis between probit value by inhibiting rate and the logarithm value of series concentration, obtain the EC of each medicament
50Value, then method abundant according to Sun Yun calculated co-toxicity coefficient (CTC).
Drug effect is calculated: two diameters are surveyed in each bacterium colony right-angled intersection, with its mean, represent the bacterium colony size.Obtain the colony growth inhibiting rate by following formula: colony growth inhibiting rate %=(blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony growth diameter
The co-toxicity coefficient computational methods are with biologicall test embodiment 1.
The two azoles of phenylate and the composite Toxicity Determination to vegetables black spot, downy mildew of fluoxastrobin the results are shown in table 5 and table 6.
Table 5: the two azoles of phenylate and the composite result of Toxicity Determination to the Chinese cabbage black spot of fluoxastrobin
Table 6: the two azoles of phenylate and the composite result of Toxicity Determination to Chinese cabbage downy mildew of fluoxastrobin
Be can clearly be seen that by above-mentioned Toxicity Determination result, the two azoles of phenylate are mixed with fluoxastrobin, in mass ratio is the scope of 100:1～1:100, to potato morning, late blight, wheat powdery mildew, rust, vegetable leaf pinta and downy mildew all show as synergistic effect.Wherein synergistic effect is the most obvious in mass ratio is the scope of 10:1～1:10.Result shows, the two azoles of phenylate and fluoxastrobin are mixed and prevent and treat above-mentioned disease and have reasonability and feasibility.
Two, Preparation Example
In all preparation proportionings, percentage composition is mass percent.
Embodiment 1: prepare water dispersible granules
Concrete preparation method is as follows: press formula rate; former medicine and dispersant, wetting agent, binding agent, filler etc. are mixed, obtain pulvis after air-flow crushing, add a certain amount of water to mix; carry out granulation in comminutor, then dry, screening namely obtains granular product.
The two azoles fluoxastrobin water dispersible granules preparations of 40% phenylate:
Take the two former medicines of azoles of 25% phenylate, the 15% former medicine of fluoxastrobin, 3%NNO(alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 4%K-12(lauryl sodium sulfate), 5% carboxymethyl (ethyl) cellulose, 3% diatomite, 5% glucose, kaolin add to 100%.Mix, granulate after air-flow crushing.
Embodiment 2: prepare suspending agent
Concrete preparation method is as follows:, by following formula rate,, take water as medium, former medicine, dispersant, suspending agent and antifreezing agent etc. are joined in high-shear emulsion machine and shear 30min, then after using the sand mill sand milling, obtain suspending agent.
The two azoles fluoxastrobin suspending agent preparations of 40% phenylate:
Take the two former medicines of azoles of 25% phenylate, the 15% former medicine of fluoxastrobin, 4%FS3000,1%JFC(fatty alcohol-polyoxyethylene ether), 0.2% xanthans, 1% aluminium-magnesium silicate, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organically-modified silica alkane defoamer, deionized water adds to 100%.Make slurry, carry out sand milling, obtain described product.
Embodiment 3: prepare suspension emulsion
Concrete preparation method is as follows: by following formula rate, former medicine and dispersant, wetting agent, defoamer, water etc. are mixed, through the sand mill sand milling, obtain the suspension mother liquor; Again another former medicine, solvent, emulsifier etc. are mixed, obtain the homogeneous oil phase,, according to metering proportion, under the condition of high shear, oil phase and excess water are joined in the suspension mother liquor and mix and shear evenly.Add again thickener and preservative to stir and obtain required product.
The two azoles fluoxastrobin suspension emulsion preparations of 30% phenylate:
The two former medicines of azoles of 25% phenylate, the 5% former medicine of fluoxastrobin, 3%FS3000,1%SXC(sulfonic acid type anion surfactant), 30% solvent naphtha S150,5% agriculture breast 500,1%NS-500LQ, 0.1% xanthans, 5% ethylene glycol, 0.03%BIT, 0.3% organically-modified silica alkane defoamer, deionized water adds to 100%.First the two former medicines of azoles of phenylate, FS3000, SXC, ethylene glycol, defoamer, part deionized water are mixed, in the sand mill sand milling, obtain the two azoles suspension mother liquors of phenylate.Again the former medicine of fluoxastrobin, solvent naphtha, emulsifiers dissolve are mixed and obtain the homogeneous oil phase,, according to metering proportion, under the condition of high shear, oil phase and excess water are joined in the suspension mother liquor and mix and shear evenly.Add again thickener xanthans and preservative BIT to be uniformly mixed and obtain required product.
Embodiment 4: prepare wetting powder
, by following formula requirement, after various auxiliary agents, filler, former medicine are mixed and pulverize by Roughpulverizer, enter airslide disintegrating mill and fully be pulverized and mixed and make wetting powder.
The two azoles fluoxastrobin wetting powder preparations of 80% phenylate:
Take the two former medicines of azoles of 50% phenylate, the 30% former medicine of fluoxastrobin, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12, kaolin and add to 100%.Mix, obtain product after pulverizing.
Embodiment 5: prepare microemulsion
The two azoles fluoxastrobin microemulsion preparations of 25% phenylate:
Take the two former medicines of azoles of 25% phenylate, the 10% former medicine of fluoxastrobin, 5% Nongru-700 #, 5% agriculture breast 500#, 6% agriculture breast 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolve complete and mix, deionized water adds to 100%.After stirring, obtain product.
Embodiment 6: prepare aqueous emulsion
The two azoles aqueous emulsion preparations of 10% fluoxastrobin phenylate:
Take the 5% former medicine of fluoxastrobin, the two former medicines of azoles of 5% phenylate, 3% agriculture breast 2201#, 1.5% Nonyl pheno (EO=10) ether phosphate, 2.5% agriculture breast 600# acid phosphatide, 20% cyclohexanone, 10% solvent naphtha S150, mixed dissolution obtains oil phase.With 5% ethylene glycol, 0.3% xanthans, 0.5% benzoic acid, deionized water adds to 100%, is made into water.Oil phase adds aqueous phase fully to mix, and after high-shear emulsifying, obtains product.
Three, field application test
Application Example 1: the control potato early, the late blight field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.34-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating potato late blight ", the residential quarter of test medicine, contrast medicament and blank is processed and is adopted random district group to arrange, residential quarter area 15m
2, 4 repetitions, target, the dispenser of late blight their early stage, take twice dispenser (squaring period and florescence), interval 14d, the 15d investigation potato leaf section incidence of disease, disease index after the secondary dispenser.5 samplings of diagonal are adopted in every experimental plot, every some investigation 2 ~ 3 strains, and whole blades of the every strain of investigation, record the disease grade.
The disease scale standard is as follows:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Drug effect computational methods:, according to investigation result, according to following (1), (2) formula, calculate disease index and preventive effect.
In formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
The control potato early, the late blight field control effectiveness test the results are shown in table 7.
Table 7: the control potato is late blight field control effectiveness test result early
Application Example 2: control wheat powdery mildew field control effectiveness test
The residential quarter of test medicine, contrast medicament and blank is processed and is adopted random district group to arrange, residential quarter area 20 square meters, 4 repetitions, each spraying control of booting stage and flowering initial stage 1 time.This tests the investigation in the 15th day after dispenser for the second time, adopts random five point samplings, gets 20 strains at every, upper 3 leaves (having boot leaf to comprise boot leaf) of the every strain of investigation.
The disease scale standard is as follows:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 15% of whole leaf area;
5 grades: lesion area accounts for 16% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
Drug effect computational methods:, according to investigation result, according to following (1), (2) formula, calculate disease index and preventive effect.
In formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control wheat powdery mildew field control effectiveness test the results are shown in table 8.
Table 8: control wheat powdery mildew field control effectiveness test result
Application Example 3: prevent and treat the wheat rust field control effectiveness test
The residential quarter of test medicine, contrast medicament and blank is processed and is adopted random district group to arrange, residential quarter area 20 square meters, and 4 repetitions, this tests dispenser at the initial stage of a disease 1 time.After medicine, 30 days investigation drug effects, take 5 samplings of every residential quarter diagonal during investigation, and 20 leaves of every some investigation, amount to 100 leaves, records the sick level of different disposal according to stage division, calculates disease index and control efficiency.
The disease scale standard is as follows:
0 grade: anosis
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～25% of one-piece blade area:
5 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
7 grades: lesion area accounts for 51% ~ 75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
, according to investigation result, according to following (1), (2) formula, calculate disease index and preventive effect.
In formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Prevent and treat the wheat rust field control effectiveness test and the results are shown in table 9.
Table 9: prevent and treat wheat rust field control effectiveness test result
Application Example 4: control Chinese cabbage black spot field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.27-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating vegetable leaf pinta ", the residential quarter of test medicine, contrast medicament and blank is processed and is adopted random district group to arrange, residential quarter area 15m
2, 4 repetitions, the dispenser for the first time of disease initial stage, interval 7d secondary dispenser, 7d Investigate incidence, disease index after the secondary medicine.5 samplings of diagonal are adopted in every experimental plot, every some investigation 2 ~ 3 strains, and whole blades of the every strain of investigation, record the disease grade.
Disease disease scale standard is following:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
The drug effect computational methods:
, according to investigation result, according to following (1), (2) formula, calculate disease index and preventive effect.
In formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control Chinese cabbage black spot field control effectiveness test the results are shown in table 10.
Table 10: control Chinese cabbage black spot field control effectiveness test result
Application Example 5: control Chinese cabbage downy mildew field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.115-2004 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating Chinese cabbage downy mildew ", the residential quarter of test medicine, contrast medicament and blank is processed and is adopted random district group to arrange, residential quarter area 15m
2, 4 repetitions, the dispenser for the first time of disease initial stage, interval 7d secondary dispenser, 7d Investigate incidence, disease index after the secondary medicine.5 samplings of diagonal are adopted in every experimental plot, every some investigation 3 ~ 4 strains, and whole blades of the every strain of investigation, record the disease grade.
The disease scale standard is as follows:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
The drug effect computational methods:
, according to investigation result, according to following (1), (2) formula, calculate disease index and preventive effect.
In formula:
CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control Chinese cabbage downy mildew field control effectiveness test the results are shown in table 11.
Table 11: control Chinese cabbage downy mildew field control effectiveness test result
Comprehensively above-mentioned, a large amount of indoor biometricses and field control effectiveness test result show that the beneficial effect of Bactericidal composite deposits yields of the present invention is:
(1) compare with single dose, this bactericidal composition has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, lower drug cost, environmental contamination reduction and residue of pesticide;
(3) composition is comprised of the active ingredient of different mechanism of action, and action site increases, and is conducive to overcome and delays the drug-fast generation of germ.The present composition is to trial crops all without obviously harmful effect, and leaf look, growing way etc. are all normal, and safety is good, meets the security requirement of pesticidal preparations.
Bactericidal composition of the present invention has good preventive effect to target and late blight, wheat powdery mildew and rust, vegetable leaf pinta and downy mildew.
Claims (7)
1. bactericidal composition that contains the two azoles of phenylate and fluoxastrobin is characterized in that this bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
B is fluoxastrobin;
Wherein the weight ratio of component A and B component is 1:100～100:1.
2. bactericidal composition according to claim 1, is characterized in that in this bactericidal composition, the weight ratio of component A and B component is 1:50～50:1.
3. bactericidal composition according to claim 1, is characterized in that in this bactericidal composition, the weight ratio of component A and B component is 1:10～10:1.
4. bactericide, it is characterized in that it is acceptable carrier and auxiliary agent on agricultural chemicals that this bactericide contains the described bactericidal composition of any one claim and surplus in the claim 1-3 of 0.1～90 % by weight, this bactericide is mixed with suspending agent, suspension emulsion, aqueous emulsion, microemulsion, wetting powder or water dispersible granules.
5. bactericide according to claim 4, is characterized in that this bactericide contains the described bactericidal composition of any one claim in the claim 1-3 of 10～80 % by weight.
6. bactericide according to claim 4, is characterized in that this bactericide contains the described bactericidal composition of any one claim in the claim 1-3 of 28～62 % by weight.
7. the described bactericidal composition of any one claim is being prevented and treated target, late blight, wheat rust, powdery mildew, the purposes in vegetable leaf pinta, downy mildew according to claim 1-3.
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| EP2925730A1 (en) | 2012-11-27 | 2015-10-07 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| US20160029630A1 (en) | 2012-11-27 | 2016-02-04 | Basf Se | Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides |
| EP2783569A1 (en) * | 2013-03-28 | 2014-10-01 | Basf Se | Compositions comprising a triazole compound |
| CN106465725A (en) * | 2016-08-29 | 2017-03-01 | 南京华洲药业有限公司 | A kind of bactericidal composition containing fluoxastrobin and simeconazoles and its application |
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| CN1923819A (en) * | 2006-10-12 | 2007-03-07 | 青岛科技大学 | compound containing aromatic ether and ditriazole and use thereof |
| CN101039578A (en) * | 2004-10-12 | 2007-09-19 | 拜尔农作物科学股份公司 | Fungicidal active ingredient combinations containing fluoxastrobin |
| CN101485307A (en) * | 2009-02-09 | 2009-07-22 | 吉林省八达农药有限公司 | Agricultural fungicidal composition containing SYP-3375 and triazole fungicide |
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| CN1923819A (en) * | 2006-10-12 | 2007-03-07 | 青岛科技大学 | compound containing aromatic ether and ditriazole and use thereof |
| CN101485307A (en) * | 2009-02-09 | 2009-07-22 | 吉林省八达农药有限公司 | Agricultural fungicidal composition containing SYP-3375 and triazole fungicide |
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