CN102715173A - Bactericidal composition containing phenylate diazole and fluoxastrobin and application of bactericidal composition - Google Patents

Bactericidal composition containing phenylate diazole and fluoxastrobin and application of bactericidal composition Download PDF

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CN102715173A
CN102715173A CN2012102239569A CN201210223956A CN102715173A CN 102715173 A CN102715173 A CN 102715173A CN 2012102239569 A CN2012102239569 A CN 2012102239569A CN 201210223956 A CN201210223956 A CN 201210223956A CN 102715173 A CN102715173 A CN 102715173A
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bactericidal composition
fluoxastrobin
bactericide
phenylate
disease
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CN102715173B (en
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吴华龙
徐黎婷
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Zhejiang Yulong Pharmaceutical Co ltd
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HANGZHOU UDRAGON CHEMICAL CO Ltd
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Abstract

The invention relates to a bactericidal composition and application thereof. The bactericidal composition contains phenylate diazole and fluoxastrobin and is used for controlling potato early blight and later blight, wheat powdery mildew and rust disease, vegetable leaf spot disease and downy mildew. Compared with a single bactericide, the composition has an obvious synergistic effect and is favorable for overcoming and delaying the resistance of bacteria.

Description

A kind of bactericidal composition that contains two azoles of phenylate and fluoxastrobin and uses thereof
[technical field]
The invention belongs to technical field of pesticide.More specifically, the present invention relates to a kind of bactericidal composition that contains two azoles of phenylate and fluoxastrobin, also relate to the purposes of said bactericidal composition.
[background technology]
Potato late blight (Phytophthora infestans) is to cause a kind of destructive popular disease that potato haulm is dead and stem tuber rots, and is caused by phytophthora infestans, and for just infecting the source, it requires the nice and cool weather of high humidity with the disease potato that carries disease germs.Under suitable condition, germ rapidly breeding repeatedly infect again cause popular; Target (Alternaria solani) pathogen is an epidemic disease Alternaria early, except that the harm potato, can also endanger the vegetables such as tomato, capsicum of booth and greenhouse culture.Germ at invalid body with survive the winter on the potato piece in spite of illness, was infected morbidity with conidium or mycelia when next year, potato seed began to germinate.Potato raw meets cloudy weather for several days running weather for a long time or humidity is higher than 70% continuously, and it is serious even popular to fall ill, and this disease is in rising trend in recent years.Early blight and late blight are often produced to potato and are caused great loss.
Wheat rust is jaundice again, and 3 kinds of yellow rust, black stem rust and leaf rust are arranged.Be that distribution is wide on China's Wheat Production, propagation is fast, the important disease that hazard area is big.Wherein take place the most general and serious with stripe rust of wheat.After the wheat generation rust, nutrient is absorbed in the body, and chlorophyll is destroyed, and a large amount of soruss are broken through wheat leaf, straw epidermis, have a strong impact on wheat yield and quality; Wheat powdery mildew (Blumeria graminis) is a kind of worldwide disease, in each main wheat producing countries distribution is arranged all, and China's Shandong coastal waters, Sichuan, Guizhou, Yunnan take place general, and it is also heavy to cause harm.In recent years should disease northeastward, North China, northwest Mai Qu, the gesture that is on the rise is also arranged.This disease can be encroached on each organ of wheat plant overground part, but is main with blade and leaf sheath, and grain husk shell and awns also can be injured when morbidity was heavy.Seedling stage to becoming the strain phase all can cause harm, main harm blade, also can cause harm when serious leaf sheath, stem stalk and fringe portion.General face of blade scab is more than reverse side, and lower blade is more than upper blade, and scab can heal for a long time in flakes.And cause yellow leaf withered.After stem stalk and leaf sheath were injured, plant was prone to lodging.
The vegetable leaf pinta is that vegetables produce upward one of important disease, and it is wider to distribute, and all there is generation various places, generally take place heavier in spring, two seasons of autumn.The incidence of disease 10%~60%, serious plot or canopy chamber reach 100%, but the underproduction 10%~30%, but the underproduction more than 60% during especially severe.This disease vegetables such as muskmelon, cucumber, wax gourd, sponge gourd, balsam pear of also can causing harm.The false pseudomonas bacillus of the Pseudomonas of causing a disease.Vegetables downy mildew is one type of most important disease on the protection ground vegetables, can cause the underproduction 20%~30% generally speaking, can reach more than 50% when serious, and along with the expansion of protecting Di Peng chamber area, its harm is more and more heavy.
The inventor discovers; With two azoles of phenylate and fluoxastrobin is the bactericidal composition of active ingredient, is used for target and late blight, the control of wheat rust and powdery mildew, vegetable leaf pinta and downy mildew; Can delay the drug-fast generation of germ; Improving actual preventive effect, enlarge fungicidal spectrum and reduce drug cost, is the important means of disease integrated control.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of bactericidal composition that contains two azoles of phenylate and fluoxastrobin.
Another object of the present invention provides the purposes of said bactericidal composition.
[technical scheme]
The present invention realizes through following technical proposals.
The present invention relates to a kind of bactericidal composition that contains two azoles of phenylate and fluoxastrobin.This bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
Figure BDA00001819554000021
The two azoles of phenylate (English common name: Difenodiconazole), chemical name: 2-[2-chloro-4-(4-chlorobenzene oxygen) phenyl]-1, two (1H-1,2,4-the triazol-1-yl)-2-propyl alcohol of 3-.CN 200610140836.7 discloses chemical structural formula, preparation method and the sterilizing use of the two azoles of phenylate, in present specification, this patent application is incorporated herein by reference in full.
B is a fluoxastrobin;
Fluoxastrobin (Fluoxastrobin) is that novel, the interior absorption cauline leaf of wide spectrum Er Qing oxazine class of Bayer Cropscience Co.,Ltd's report handled bactericide.Its application optimum period, is wide, and no matter early stage the as spore germination at fungal infection, germ tube growth still can both provide extraordinary protection and therapeutic action at growth period of hypha, and be especially very effective to spore germination and initial stage dip-dye.Because of the systemic activity of its excellence that has, so it can be by fast Absorption, and can transmit to the top equably in leaf portion, so have good resistance of rainwater washing against ability.Fluoxastrobin is mainly used in cauline leaf and handles; Can prevent and treat nearly all Mycophytes (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease in cereal crop, potato, vegetables and the coffee etc. effectively, for example eqpidemic disease, rust, Ying's rot, net blotch, powdery mildew, leaf spot or downy mildew etc.Can prevent and treat target and late blight very effectively; Can prevent and treat vegetable leaf pinta, downy mildew very effectively.Can prevent and treat cereal crop leaf spot, glume blight, leaf rust, yellow rust, moire disease, brown spot, net blotch very effectively, powdery mildew is also had good drug effect, and can the double full rot of controlling.
In bactericidal composition of the present invention, the weight ratio of said component A and said B component is 1:100 ~ 100:1.
Preferably, the weight ratio of component A and B component is 1:50~50:1 in this bactericidal composition.
More preferably, the weight ratio of component A and B component is 1:10~10:1 in this bactericidal composition.
The invention still further relates to bactericidal composition of the present invention and preventing and treating target, late blight, wheat rust, powdery mildew, the purposes in vegetable leaf pinta, the downy mildew.
The present invention relates to a kind of bactericide.
This bactericide contains the above-mentioned bactericidal composition of 0.1~90 weight % and surplus is acceptable carrier and an auxiliary agent on the agricultural chemicals.
Preferably, this bactericide contains the above-mentioned bactericidal composition of 10~80 weight %.
More preferably, this bactericide contains the above-mentioned bactericidal composition of 28~62 weight %.
Except as otherwise noted, " % " involved in the present invention is " percentage by weight ".
Can bactericide involved in the present invention be mixed with agricultural and go up acceptable forms, preferred dosage form is suspending agent, suspension emulsion, aqueous emulsion, microemulsion, wetting powder or water dispersible granules.All these formulations all adopt method as well known to those skilled in the art to prepare by acceptable carrier and auxiliary agent on above-mentioned bactericidal composition and the agricultural chemicals.
As for the water-dispersible grain formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of dispersant, wetting agent, disintegrant, binding agent and filler.
Described dispersant is selected for use from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from polyoxyethylene alcohol, alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described disintegrant is selected from ammonium sulfate, urea, sucrose, glucose, citric acid, succinic acid or sodium bicarbonate.
Described binding agent is selected from diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family and microcrystalline cellulose class.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, sepiolite, talcum powder, attapulgite or potter's clay etc.
Employed dispersant, wetting agent, disintegrant, binding agent and filler all are product solds in the market when preparation water-dispersible grain formulation.
As for the wettable powder formulation, those skilled in the art can adopt conventional preparation method to be obtained by the main auxiliary agent preparation of dispersant, wetting agent and filler.
Described dispersant is selected from polycarboxylate, lignosulfonates or alkylnaphthalene sulfonate.
Described wetting agent is selected from alkyl sulfate, alkylsulfonate or naphthalene sulfonate.
Described filler is selected from diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite or potter's clay etc.
Employed dispersant, wetting agent and filler all are product solds in the market when preparation wettable powder formulation.
As for suspending agent type, those skilled in the art can adopt conventional preparation method to be obtained by these auxiliary agent preparations of dispersant, wetting agent, thickener, preservative, antifoaming agent and antifreezing agent.
Described dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate or TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt).
Described wetting agent is selected from alkylphenol-polyethenoxy and gathers basic ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate or TERSPERSE 2500 (U.S. Huntsman Corporation product).
Described thickener is selected from xanthans, aluminium-magnesium silicate or bentonite.
Described preservative is selected from benzoic acid, Sodium Benzoate or BIT (1, the 2-benzisothiazole-3-ketone).
Described antifoaming agent is silicone based antifoaming agent.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Preparation during suspending agent type employed dispersant, wetting agent, thickener, preservative, antifoaming agent and antifreezing agent all be product sold in the market.
As for the suspension emulsion type, those skilled in the art can adopt conventional preparation method to be obtained by these auxiliary agent preparations of dispersant, wetting agent, emulsifier, solvent, thickener, preservative, antifoaming agent and antifreezing agent.
Described dispersant is selected from polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate or TERSPERSE 2425 (U.S. Huntsman Corporation produces, the alkyl naphthalene sulfonic acid salt).
Described wetting agent is selected from alkylphenol-polyethenoxy and gathers basic ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate or TERSPERSE 2500 (U.S. Huntsman Corporation product).
Described emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# phosphate), Nongru-700 #, agricultural newborn 2201#, Span-60#, emulsifier T-60, TX-10, agricultural newborn 1601#, agricultural newborn 600#, agricultural newborn 400#;
Described solvent is selected from xylol, toluene, cyclohexanone or solvent naphtha (trade mark: S-150, S-180, S-200);
Described thickener is selected from xanthans, aluminium-magnesium silicate or bentonite.
Described preservative is selected from benzoic acid, Sodium Benzoate or BIT (1, the 2-benzisothiazole-3-ketone).
Described antifoaming agent is silicone based antifoaming agent.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Preparation during suspending agent type employed dispersant, wetting agent, emulsifier, solvent, thickener, preservative, antifoaming agent and antifreezing agent all be product sold in the market.
As for the water and milk formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, solvent, stabilizing agent, antifreezing agent, thickener and preservative.
Described emulsifier is selected from Nonyl pheno (EO=10) ether phosphate, triphen ethyl-phenol polyoxyethylene ether phosphate (agricultural newborn 600# phosphate), Nongru-700 #, agricultural newborn 2201#, Span-60#, emulsifier T-60, TX-10, agricultural newborn 1601#, agricultural newborn 600# or agricultural newborn 400#.
Described solvent is selected from xylol, toluene, cyclohexanone or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
Described antifreezing agent is selected from ethylene glycol, propane diols, glycerine, urea or inorganic salts such as sodium chloride.
Described thickener is selected from xanthans, bentonite or aluminium-magnesium silicate.
Described preservative is selected from BIT (1, the 2-benzisothiazole-3-ketone), benzoic acid or Sodium Benzoate.
Employed emulsifier, solvent, stabilizing agent, antifreezing agent, thickener and preservative all are product solds in the market when preparation water and milk formulation.
As for the micro emulsion formulation, those skilled in the art can adopt conventional preparation method to be obtained by these the main auxiliary agent preparations of emulsifier, cosolvent, solvent and stabilizing agent.
Described emulsifier is selected from calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 #, agricultural newborn 2201#, this dish-60#, Tween-80, TX-10, farming breast 1601, agricultural newborn 600# or agricultural newborn 400#.
Described cosolvent is selected from methyl alcohol, isopropyl alcohol, n-butanol or ethanol.
Described solvent is selected from cyclohexanone, N-Methyl pyrrolidone, xylol, toluene or solvent naphtha (trade mark: S-150, S-180, S-200).
Described stabilizing agent is selected from triphenyl phosphite or epoxychloropropane.
Employed emulsifier, cosolvent, solvent and stabilizing agent all are product solds in the market when preparation micro emulsion formulation.
Bactericidal composition of the present invention can provide with the finished product preparation form, and promptly each material mixes in the composition; Also can provide, in bucket (jar), directly mix before using with single agent formulation.Concentrate of the present invention mixes the concentration that obtains required active substance usually with water.
The invention still further relates to a kind of target, late blight of preventing and treating; Wheat rust, powdery mildew; The method of vegetable leaf pinta, downy mildew disease; Before or after potato, wheat or vegetable crop introduced disease, use above-mentioned bactericidal composition of the present invention or bactericide to the place of its growth or storage.Its application process is the conventional application process of agricultural chemicals, for example waters, sprays, spraying, dusting, broadcasts sowing etc.
[beneficial effect]
The present invention has following beneficial effect:
(1) compare with single agent, bactericidal composition according to the invention has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, reduce drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.
[embodiment]
Can understand the present invention better through following embodiment.As not having specified otherwise, active ingredient ratio of the present invention is weight ratio.
Embodiment 1
One, biological activity determination
Embodiment 1: the composite Toxicity Determination to potato morning, late blight of the two azoles of phenylate and fluoxastrobin
Subjects target (Alternaria solani) and potato late blight (Phyophthora infestans).
Adopt the mycelial growth rate method to measure the composite inhibitory action of the two azoles of phenylate and the former medicine of fluoxastrobin and the two different proportionings of azoles of phenylate to potato morning, late disease bacteria with fluoxastrobin.With tested bacterial strain after cultivating 6d on the PDA flat board; Beat cut-off from colony edge and directly be the bacterium cake of 5mm, the bacterium cake is received on the flat board that contains different quality concentration medicament, contrast adds sterile water; Every processing repetition 3 times; Place 25 ℃, every day the 12h dark and the condition of 12h illumination under cultivate 6d (late blight is the insulating box dark culturing 8~10d 18 ℃ of temperature, colony diameter to be contrasted be at least 50mm above but when not covering with whole culture dish), adopt the right-angled intersection method to measure and respectively handle colony diameter.Calculate and respectively handle bacterium colony growth diameter and inhibiting rate, The data DPS software carries out statistical analysis.
Figure BDA00001819554000081
Mass concentration logarithm to set is an abscissa, and inhibiting rate probability value is an ordinate, obtains virulence regression equation, correlation coefficient r and in effective the suppress concentration EC of each medicament to supplying the examination germ 50Value.
The co-toxicity coefficient computational methods: according to the abundant method of Sun Yun (document ... ... each EC that handles that ..) will measure 50Be converted into actual poison exponent (ATI); According to the proportioning of mixture, obtain theoretical poison exponent (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Figure BDA00001819554000082
Theory is used the content (%) of toxicity index * A in using with of toxicity index (TTI)=A with
The content (%) of toxicity index * B of+B in using with
Figure BDA00001819554000084
If co-toxicity coefficient, shows two azoles of phenylate and fluoxastrobin mixture greater than 120 synergistic effect is arranged; If co-toxicity coefficient is starkly lower than 100, show that its mixture is an antagonism; If co-toxicity coefficient is 100~120, show that its mixture is a summation action.
The two azoles of said phenylate and fluoxastrobin composite to potato early, the Toxicity Determination of late blight lists in table 1 and the table 2.
Table 1: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to target
Figure BDA00001819554000085
Figure BDA00001819554000091
Table 2: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to potato late blight
Embodiment 2: two azoles of phenylate and the composite Toxicity Determination to wheat powdery mildew, rust of fluoxastrobin
Powdery mildew test target wheat powdery mildew (Erysiphe graminis) is cultivated by the long-term live body of this laboratory and is selected for use susceptible variety (raising wheat No. 6) potted plant; Treat that seedling grows to 2 leaves~3 leaf phases; Spraying is handled the back and is dried naturally, and the fresh spore of white powder germ that the wheat leaf blade of falling ill was produced in last 24 hour is evenly shaken off to be inoculated on the potted plant wheat seedling of 2~3 leaf phases that chemicals treatment crosses.Postvaccinal examination material moves to 20 ℃, relative moisture 80% left and right sides controllable greenhouse glass room is interior cultivates.Note keeping basin soil moistening every day, investigate according to the classification of blank incidence about 7 days.With reference to pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating cereal class powdery mildew.
The disease grade scale is following:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 16% ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are with instance 1.
Two azoles of phenylate and the composite Toxicity Determination result to wheat powdery mildew of fluoxastrobin list in the table 3.
Table 3: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to wheat powdery mildew
Figure BDA00001819554000101
The rust experiment target is designated as wheat leaf rust pathogen (Puccinia recondita), and wheat breed is for raising wheat No. 6, and every basin is selected about l0 strain wheat seeding for use; Treat long subsequent use to the 3-4 leaf phase; Supply reagent agent and proportioning medicament to be mixed with the soup that presets concentration, 4 repetitions are established in the processing of spraying, every processing.Get the wheat leaf blade that covers with the leaf rust bacterium behind the 24h, wash gently with sterile water and get surperficial fresh spore, filter with double gauze, processing spore concentration is the suspension about 100,000/ml, spray inoculation (pressure 0.1MPa).Wheat seeding was preserved moisture one day under 20 ℃ of conditions after connecing bacterium, put back to the greenhouse again, when waiting to contrast abundant morbidity after the week, carried out state of an illness investigation by field control effectiveness test criterion grade scale, referred to calculate preventive effect by disease.Obtain EC with return law of the straight line 50
The disease grade scale is following:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 25% of one-piece blade area;
5 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
7 grades: lesion area accounts for 51% ~ 75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
Preventive effect and co-toxicity coefficient computational methods are with instance 1.
Two azoles of phenylate and the composite Toxicity Determination result to wheat rust of fluoxastrobin list in the table 4.
Table 4: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to wheat rust
Embodiment 3: two azoles of phenylate and the composite Toxicity Determination to vegetable leaf pinta, downy mildew of fluoxastrobin
Subjects: the leaf spot target is Chinese cabbage black spot (Alternaria brassicae), Chinese cabbage downy mildew (Peronospora parasitica).
Test method: with reference to " the agricultural industry criteria NY/T 1156.2-2006 of the People's Republic of China (PRC) " mycelial growth rate method.Each medicament is established 5 dosage respectively by active constituent content and is handled; Above-mentioned each pathogen is used the PDA medium culture; When treating that bacterium colony has just covered with culture dish, break into the bacterium piece at colony edge, the bacterium piece is moved to the toxic PDA medium central authorities that are mixed with in advance with transfer needle with the card punch of diameter 5mm; Place then in 25 ℃ of incubators and cultivate every processing repetition 4 times.Look CK colony growth situation, adopt the right-angled intersection method to measure and respectively handle colony diameter cm, obtain to proofread and correct and suppress percentage with slide calliper rule.The EC of each medicament is obtained in linear regression analysis between the probit value through inhibiting rate and the logarithm value of series concentration 50Value is calculated co-toxicity coefficient (CTC) according to the abundant method of Sun Yun again.
Drug effect is calculated: two diameters are surveyed in each bacterium colony right-angled intersection, represent the bacterium colony size with its mean.Obtain the colony growth inhibiting rate by following formula: colony growth inhibiting rate %=(the blank bacterium colony increases diameter-chemicals treatment bacterium colony and increases diameter) * 100/ blank bacterium colony increases diameter
The co-toxicity coefficient computational methods are with biologicall test embodiment 1.
Two azoles of phenylate and the composite Toxicity Determination result to vegetables black spot, downy mildew of fluoxastrobin list in table 5 and the table 6.
Table 5: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to the Chinese cabbage black spot
Figure BDA00001819554000121
Table 6: two azoles of phenylate and the composite Toxicity Determination result of fluoxastrobin to Chinese cabbage downy mildew
Figure BDA00001819554000122
Figure BDA00001819554000131
Can clearly be seen that by above-mentioned Toxicity Determination result; The two azoles of phenylate are mixed with fluoxastrobin, in mass ratio is the scope of 100:1~1:100, to potato morning, late blight; Wheat powdery mildew, rust, vegetable leaf pinta and downy mildew all show as synergistic effect.Wherein synergistic effect is the most obvious in mass ratio is the scope of 10:1~1:10.The result shows, the two azoles of phenylate and fluoxastrobin are mixed and prevent and treat above-mentioned disease and have reasonability and feasibility.
Two, preparation embodiment
Percentage composition is mass percent in all preparation proportionings.
Embodiment 1: the preparation water dispersible granules
Concrete preparation method is following: press formula rate; Former medicine and dispersant, wetting agent, binding agent, filler etc. are mixed, behind comminution by gas stream, obtain pulvis, add a certain amount of water and mix; In comminutor, carry out granulation, dry then, screening promptly gets granular product.
The two azoles fluoxastrobin water dispersible granules preparations of 40% phenylate:
Take by weighing the two former medicines of azoles of 25% phenylate, the former medicine of 15% fluoxastrobin, 3%NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 4%K-12 (lauryl sodium sulfate), 5% carboxymethyl (ethyl) cellulose, 3% diatomite, 5% glucose, kaolin and add to 100%.Mix, behind comminution by gas stream, granulate.
Embodiment 2: the preparation suspending agent
Concrete preparation method is following: by following formula rate, be medium, former medicine, dispersant, suspending agent and antifreezing agent etc. joined shear 30min in the high-shear emulsion machine with water, use the sand mill sand milling again after, obtain suspending agent.
The two azoles fluoxastrobin suspending agent preparations of 40% phenylate:
Take by weighing the two former medicines of azoles of 25% phenylate, the former medicine of 15% fluoxastrobin, 4%FS3000,1%JFC (AEO), 0.2% xanthans, 1% aluminium-magnesium silicate, 5% ethylene glycol, 0.3% benzoic acid, 0.5% organically-modified silicone antifoam agent, deionized water adds to 100%.Process slurry, carry out sand milling, obtain described product.
Embodiment 3: the preparation suspension emulsion
Concrete preparation method is following: by following formula rate, former medicine and dispersant, wetting agent, antifoaming agent, water etc. are mixed, through sand mill sand milling acquisition suspension mother liquor; Again another former medicine, solvent, emulsifier etc. are mixed, obtain the homogeneous oil phase, according to metering proportion, under high shear conditions, with oil phase and excess water join mix in the suspension mother liquor shear even.Adding thickener and preservative again stirs and obtains required product.
The two azoles fluoxastrobin suspension emulsion preparations of 30% phenylate:
The two former medicines of azoles of 25% phenylate, the former medicine of 5% fluoxastrobin, 3%FS3000,1%SXC (sulfonic acid type anion surfactant), 30% solvent naphtha S150,5% farming breast 500,1%NS-500LQ, 0.1% xanthans, 5% ethylene glycol, 0.03%BIT, 0.3% organically-modified silicone antifoam agent, deionized water adds to 100%.Earlier the two former medicines of azoles of phenylate, FS3000, SXC, ethylene glycol, antifoaming agent, part deionized water are mixed, obtain the two azoles suspension mother liquors of phenylate in the sand mill sand milling.Again the former medicine of fluoxastrobin, solvent naphtha, emulsifiers dissolve are mixed and obtain the homogeneous oil phase, according to metering proportion, under high shear conditions, with oil phase and excess water join mix in the suspension mother liquor shear even.Adding thickener xanthans and preservative BIT again mixes and obtains required product.
Embodiment 4: the preparation wetting powder
By following prescription requirement, various auxiliary agents, filler, former medicine are mixed after after the Roughpulverizer pulverizing, get into airslide disintegrating mill and fully pulverize the mixed wetting powder that gets.
The two azoles fluoxastrobin wetting powder preparations of 80% phenylate:
Take by weighing the two former medicines of azoles of 50% phenylate, the former medicine of 30% fluoxastrobin, 5% calcium lignosulfonate, 2% Nekal BX, 1%K-12, kaolin and add to 100%.Mix, obtain product after the pulverizing.
Embodiment 5: the preparation microemulsion
The two azoles fluoxastrobin microemulsion preparations of 25% phenylate:
Take by weighing the two former medicines of azoles of 25% phenylate, the former medicine of 10% fluoxastrobin, 5% Nongru-700 #, 5% agricultural newborn 500#, 6% agricultural newborn 1601#, 15% cyclohexanone, 5%N-methyl pyrrolidone, 5% n-butanol, through dissolving fully and mixing, deionized water adds to 100%.After stirring, obtain product.
Embodiment 6: the preparation aqueous emulsion
The two azoles aqueous emulsion preparations of 10% fluoxastrobin phenylate:
Take by weighing the former medicine of 5% fluoxastrobin, the two former medicines of azoles of 5% phenylate, 3% agricultural newborn 2201#, 1.5% Nonyl pheno (EO=10) ether phosphate, 2.5% agricultural newborn 600# acid phosphatide, 20% cyclohexanone, 10% solvent naphtha S150, mixed dissolution obtains oil phase.With 5% ethylene glycol, 0.3% xanthans, 0.5% benzoic acid, deionized water adds to 100%, is made into water.Oil phase adds aqueous phase and fully mixes, and behind high-shear emulsifying, obtains product.
Three, field application test
Application implementation example 1: the control potato early, the late blight field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.34-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating potato late blight "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m 2, 4 repetitions, twice dispenser (squaring period and florescence) taked in target, the dispenser of late blight their early stage, interval 14d, the 15d investigation potato leaf portion incidence of disease, disease index after the secondary dispenser.5 samplings of diagonal are adopted in every experimental plot, investigate 2 ~ 3 strains at every, investigate whole blades of every strain, record disease grade.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 50% of leaf area.
Drug effect computational methods:, calculate disease index and preventive effect according to following (1), (2) formula according to investigation result.
Figure BDA00001819554000161
Figure BDA00001819554000162
In the formula:
CK 0, CK 1Be respectively the forward and backward disease index of blank district dispenser;
PT 0, PT 1Be respectively the forward and backward disease index of chemicals treatment district medicine.
The control potato early, late blight field control effectiveness test result lists in table 7.
Table 7: the control potato is late blight field control effectiveness test result early
Figure BDA00001819554000163
Application implementation example 2: control wheat powdery mildew field control effectiveness test
The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 20 square meters, 4 repetitions, each spraying control of booting stage and flowering initial stage 1 time.Five point samplings are at random adopted in investigation in the 15th day after this test dispenser for the second time, get 20 strains at every, investigate last 3 leaves (having boot leaf then to comprise boot leaf) of every strain.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 15% of whole leaf area;
5 grades: lesion area accounts for 16% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 51% of leaf area.
Drug effect computational methods:, calculate disease index and preventive effect according to following (1), (2) formula according to investigation result.
Figure BDA00001819554000171
In the formula:
CK 0, CK 1Be respectively the forward and backward disease index of blank district dispenser;
PT 0, PT 1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control wheat powdery mildew field control effectiveness test result lists in table 8.
Table 8: control wheat powdery mildew field control effectiveness test result
Figure BDA00001819554000173
Application implementation example 3: prevent and treat the wheat rust field control effectiveness test
The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 20 square meters, and 4 repetitions, this tests dispenser at the initial stage of a disease 1 time.30 days investigation drug effects are taked 5 samplings of every sub-district diagonal behind the medicine during investigation, and 20 leaves of every some investigation amount to 100 leaves, according to the sick level of stage division record different disposal, calculate disease index and control efficiency.
The disease grade scale is following:
0 grade: anosis
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%~25% of one-piece blade area:
5 grades: lesion area accounts for 26% ~ 50% of one-piece blade area;
7 grades: lesion area accounts for 51% ~ 75% of one-piece blade area;
9 grades: lesion area accounts for more than 76% of one-piece blade area.
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
In the formula:
CK 0, CK 1Be respectively the forward and backward disease index of blank district dispenser;
PT 0, PT 1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Prevent and treat wheat rust field control effectiveness test result and list in table 9.
Table 9: prevent and treat wheat rust field control effectiveness test result
Figure BDA00001819554000183
Application implementation example 4: control Chinese cabbage black spot field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.27-2000 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating vegetable leaf pinta "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m 2, 4 repetitions, the dispenser for the first time of disease initial stage, 7d secondary dispenser at interval, the 7d investigation incidence of disease, disease index behind the secondary medicine.5 samplings of diagonal are adopted in every experimental plot, investigate 2 ~ 3 strains at every, investigate whole blades of every strain, record disease grade.
Disease disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 50% of leaf area.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
Figure BDA00001819554000191
Figure BDA00001819554000192
In the formula:
CK 0, CK 1Be respectively the forward and backward disease index of blank district dispenser;
PT 0, PT 1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control Chinese cabbage black spot field control effectiveness test result lists in table 10.
Table 10: control Chinese cabbage black spot field control effectiveness test result
Figure BDA00001819554000193
Figure BDA00001819554000201
Application implementation example 5: control Chinese cabbage downy mildew field control effectiveness test
Regulation according to medicine inspecting institute of the Ministry of Agriculture " GB/T 17980.115-2004 pesticide field efficacy medicine test criterion ()---bactericidal agent for preventing and treating Chinese cabbage downy mildew "; The sub-district of test medicine, contrast medicament and blank is handled and is adopted district's group arrangement at random, sub-district area 15m 2, 4 repetitions, the dispenser for the first time of disease initial stage, 7d secondary dispenser at interval, the 7d investigation incidence of disease, disease index behind the secondary medicine.5 samplings of diagonal are adopted in every experimental plot, investigate 3 ~ 4 strains at every, investigate whole blades of every strain, record disease grade.
The disease grade scale is following:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;
5 grades: lesion area accounts for 11% ~ 20% of whole leaf area;
7 grades: lesion area accounts for 21% ~ 50% of whole leaf area;
9 grades: lesion area accounts for whole more than 51% of leaf area.
The drug effect computational methods:
According to investigation result, calculate disease index and preventive effect according to following (1), (2) formula.
Figure BDA00001819554000202
Figure BDA00001819554000203
In the formula:
CK 0, CK 1Be respectively the forward and backward disease index of blank district dispenser;
PT 0, PT 1Be respectively the forward and backward disease index of chemicals treatment district medicine.
Control Chinese cabbage downy mildew field control effectiveness test result lists in table 11.
Table 11: control Chinese cabbage downy mildew field control effectiveness test result
Figure BDA00001819554000211
Comprehensively above-mentioned, a large amount of indoor biometricses and field control effectiveness test result show that the beneficial effect that bactericidal composition according to the invention produces is:
(1) compare with single agent, this bactericidal composition has significant synergistic effect to disease, has improved bactericidal effect;
(2) reduce the medication number of times, lower drug cost, reduce environmental pollution and residue of pesticide;
(3) composition is made up of the active ingredient of different effects mechanism, and the action site increase helps overcoming and delays the drug-fast generation of germ.The present composition does not all have obvious harmful effect to trial crops, and leaf look, growing way etc. are all normal, and safety is good, meets the security requirement of pesticidal preparations.
Bactericidal composition according to the invention has good preventive effect to target and late blight, wheat powdery mildew and rust, vegetable leaf pinta and downy mildew.

Claims (8)

1. bactericidal composition that contains two azoles of phenylate and fluoxastrobin is characterized in that this bactericidal composition contains following active component A and B:
A is the two azoles of phenylate with following structural formula:
Figure FDA00001819553900011
B is a fluoxastrobin;
Wherein the weight ratio of component A and B component is 1:100 ~ 100:1.
2. bactericidal composition according to claim 1 is characterized in that the weight ratio of component A and B component is 1:50~50:1 in this bactericidal composition.
3. bactericidal composition according to claim 1 is characterized in that the weight ratio of component A and B component is 1:10~10:1 in this bactericidal composition.
4. bactericide is characterized in that this bactericide contains that the described bactericidal composition of each claim and surplus are acceptable carrier and auxiliary agent on the agricultural chemicals among the claim 1-3 of 0.1~90 weight %.
5. according to the bactericide of claim 4, it is characterized in that this bactericide contains the described bactericidal composition of each claim among the claim 1-3 of 10~80 weight %.
6. according to the bactericide of claim 4, it is characterized in that this bactericide contains the described bactericidal composition of each claim among the claim 1-3 of 28~62 weight %.
7. according to the described bactericide of each claim among the claim 3-6, it is characterized in that this bactericide is mixed with suspending agent, suspension emulsion, aqueous emulsion, microemulsion, missible oil, wetting powder or water dispersible granules.
8. preventing and treating target, late blight according to the said bactericidal composition of each claim among the claim 1-3, wheat rust, powdery mildew, the purposes in vegetable leaf pinta, the downy mildew.
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Publication number Priority date Publication date Assignee Title
WO2014082881A1 (en) 2012-11-27 2014-06-05 Basf Se Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides
WO2014082871A1 (en) 2012-11-27 2014-06-05 Basf Se Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides
EP2783569A1 (en) * 2013-03-28 2014-10-01 Basf Se Compositions comprising a triazole compound
CN106465725A (en) * 2016-08-29 2017-03-01 南京华洲药业有限公司 A kind of bactericidal composition containing fluoxastrobin and simeconazoles and its application

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CN101039578A (en) * 2004-10-12 2007-09-19 拜尔农作物科学股份公司 Fungicidal active ingredient combinations containing fluoxastrobin
CN101485307A (en) * 2009-02-09 2009-07-22 吉林省八达农药有限公司 Agricultural fungicidal composition containing SYP-3375 and triazole fungicide

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CN101039578A (en) * 2004-10-12 2007-09-19 拜尔农作物科学股份公司 Fungicidal active ingredient combinations containing fluoxastrobin
CN1923819A (en) * 2006-10-12 2007-03-07 青岛科技大学 compound containing aromatic ether and ditriazole and use thereof
CN101485307A (en) * 2009-02-09 2009-07-22 吉林省八达农药有限公司 Agricultural fungicidal composition containing SYP-3375 and triazole fungicide

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014082881A1 (en) 2012-11-27 2014-06-05 Basf Se Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides
WO2014082871A1 (en) 2012-11-27 2014-06-05 Basf Se Substituted 2-[phenoxy-phenyl]-1-[1,2,4]triazol-1-yl-ethanol compounds and their use as fungicides
EP2783569A1 (en) * 2013-03-28 2014-10-01 Basf Se Compositions comprising a triazole compound
CN106465725A (en) * 2016-08-29 2017-03-01 南京华洲药业有限公司 A kind of bactericidal composition containing fluoxastrobin and simeconazoles and its application

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