CN101911956A - Plant source pesticide and production method and application thereof - Google Patents
Plant source pesticide and production method and application thereof Download PDFInfo
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- CN101911956A CN101911956A CN2010102592585A CN201010259258A CN101911956A CN 101911956 A CN101911956 A CN 101911956A CN 2010102592585 A CN2010102592585 A CN 2010102592585A CN 201010259258 A CN201010259258 A CN 201010259258A CN 101911956 A CN101911956 A CN 101911956A
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- botanical pesticide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a plant source pesticide and a production method and application thereof. The pesticide comprises the following raw materials in percentage by weight: 30 to 50 percent of clove, 25 to 45 percent of cinnamon and 25 to 40 percent of rhubarb. The pesticide is prepared by the following steps of: crushing and mixing the raw materials; and extracting by using 70 to 90 percent ethanol, and concentrating and emulsifying to obtain the product. The pesticide has the characteristics of broad spectrum, high efficiency, low toxicity, and the like. Field test results prove that the plant source pesticide has good effect of treating cucumber fusarium wilt, cucumber downy mildew, angular leaf spot of cucumber, green pepper fusarium wilt, tomato early blight, tomato late blight or cereal crop diseases, and the like.
Description
Technical field
The present invention relates to agricultural chemicals, specifically belong to a kind of botanical pesticide and production method thereof and application.
Background technology
Plant disease is of a great variety, and the distribution region is wide, tend to agricultural production is caused very serious harm, and the gently then underproduction, heavy then can make agricultural No kernels or seeds are gathered, as in a year of scarcity, agricultural chemicals is the valuable cargo that guarantees agriculture per unit area yield.
Though chemical synthetic pesticide is being controlled and is being eliminated in the various plant diseases and brought into play enormous function for many years, but harmful substance progressively enrichment in soil and water body simultaneously, enter in crops and the people's carcass by the material circulation, severe contamination agricultural product and environment, endangered health, human cancer, hepatopathy, disease such as cardiovascular have inseparable relation therewith.
Natural products often shows many special biological natures, and widely people are used over the past thousands of years.According to statistics, the world today has 250,000 plant species at least, they can produce the metabolite that has bactericidal activity such as terpenes, alkaloid, steroid class, flavonoids and polysaccharide etc., and only accounted for 10% to what carry out research on their chemical property, as agricultural chemicals study just still less.Seek and utilize the agricultural chemicals of plant resource Development and Production controlling plant diseases to become various countries scientist's fresh target.Very abundant at China's plant resources, botanical pesticide will become the important drugs of planting the disease control from now on.The research and development botanical pesticide to the development green agriculture, promotes the sustainable development of agricultural production to have important economy, society and environmental benefit.
Summary of the invention
The object of the present invention is to provide the botanical pesticide that to prevent and treat plurality of plant diseases and the production method and the application of a kind of high-efficiency low-toxicity, non-environmental-pollution.
A kind of botanical pesticide provided by the invention makes by the following method, the raw materials by weight meter: cloves 30-50%, Chinese cassia tree 25-45%, rheum officinale 25-40%;
The preparation method comprises the steps:
1) takes by weighing above-mentioned raw materials, in proportion, pulverize, mix;
2), compound is added the 70-90% alcohol immersion 60-120min of 6-8 times of weight, be heated to 80-85 ℃ and return and heat up in a steamer 30-40min, filter, collect extract; Filter residue adds the 70-90% ethanol of 3-4 times of weight again, is heated to 80-85 ℃ time and heats up in a steamer 20-30min, filters, and collects extract, extracted twice liquid is merged obtain botanical pesticide ethanol extract just;
3), with botanical pesticide ethanol just extract obtain the botanical pesticide ethanol extract 60-65 ℃ of weight that is evaporated to compound;
4), with botanical pesticide ethanol extract and emulsifier by weight 1: 0.05-0.2 mixed product.
Described emulsifier is Tween-80 or alkylphenol polyoxyethylene class emulsifier such as OP-9, OP-10, OP-13, OP-15 or OP-20 etc.
Dilute with water 400-500 doubly sprayed to plant corpus when botanical pesticide of the present invention used, and formed one deck medicine film on the plant corpus surface, suppresses or the kill plants pathogen, thereby the plant corpus symptom is disappeared.
The results showed that botanical pesticide of the present invention has good control efficiency to plurality of plant diseases such as cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, green pepper fusarium wilt, early blight of tomato, tomato late blight or cereal crop diseases.
Embodiment
Embodiment 1
The raw material proportioning of botanical pesticide: cloves 5kg, Chinese cassia tree 2.5kg, rheum officinale 2.5kg.
Preparation process:
1, above-mentioned raw materials is pulverized, sieved, mix with 20 mesh sieves;
2, the 80% alcohol immersion 120min that compound is added 70kg is heated to 85 ℃ times and heats up in a steamer 30min in reactor, filter, and collects extract; Filter residue adds 80% ethanol of 40kg again, is heated to 85 ℃ times and heats up in a steamer 30min, filters, and collects extract, extracted twice liquid is merged obtain botanical pesticide ethanol extract just;
3, the first extract of botanical pesticide ethanol is evaporated to 10kg at 65 ℃, obtains the botanical pesticide ethanol extract;
4,10kg botanical pesticide ethanol extract and 1.5kg OP-13 mixing are made product;
5, can.
Embodiment 2
The raw material proportioning of botanical pesticide: cloves 4kg, Chinese cassia tree 3kg, rheum officinale 3kg.
Preparation process:
1, above-mentioned raw materials is pulverized, sieved, mix with 20 mesh sieves;
2, the 85% alcohol immersion 100min that compound is added 60kg is heated to 80 ℃ times and heats up in a steamer 40min in reactor, filter, and collects extract; Filter residue adds 85% ethanol of 30kg again, is heated to 80 ℃ times and heats up in a steamer 20min, filters, and collects extract, extracted twice liquid is merged obtain botanical pesticide ethanol extract just;
3, the first extract of botanical pesticide ethanol is evaporated to 10kg at 60 ℃, obtains the botanical pesticide ethanol extract;
4,10kg botanical pesticide ethanol extract and 0.9kg Tween-80 mixing are made product;
5, can.
Embodiment 3
The raw material proportioning of botanical pesticide: cloves 4.5kg, Chinese cassia tree 2.5kg, rheum officinale 3kg.
Preparation process is with embodiment 1.
Embodiment 4
The botanical pesticide control cucumber fusarium axysporum field control effectiveness test that the embodiment of the invention 1 makes:
5 processing are established in test altogether, 4 repetitions, each sub-district area 10m
2, carry out experimental scheme, wherein A according to the completely random design: the clear water blank; B: 800 times of dilution positive controls of compound carbendazim; C: 500 times of dilutions of botanical pesticide of the present invention; D: 400 times of dilutions of botanical pesticide of the present invention; E: 300 times of dilutions of botanical pesticide of the present invention.Test method is that the 300ml/ strain irritates root, perfusion 2 times, interval 7d.
Investigation content and data are handled: every sub-district is selected 30 strains at random, 7d investigation before perfusion and after the 2nd perfusion, record diseased plant number.Carry out the output investigation during results, the single receipts in every sub-district, weighing separately.
The incidence of disease (%)=(the total strain number of diseased plant number/investigation) * 100.
Control efficiency (%)=[(the check plot incidence of disease-treatment region incidence of disease)/check plot incidence of disease] * 100.
Table 1 botanical pesticide control of the present invention cucumber fusarium axysporum field test results
Result of the test shows that botanical pesticide control cucumber fusarium axysporum of the present invention is anti-all relatively good between 500 times of dilution to 300 times dilutions, and is suitable with the compound carbendazim of 800 times of dilutions.By this experimental observation, three dosage of botanical pesticide of the present invention all do not have harmful effect to cucumber growth, contrast apparently higher than clear water through the cell production of chemicals treatment.The concentration of taking all factors into consideration botanical pesticide control control cucumber fusarium axysporum of the present invention is the best with 400 times of dilutions, and the control cucumber fusarium axysporum is had positive effect.
Embodiment 5
The botanical pesticide control cucumber downy mildew field control effectiveness test that the embodiment of the invention 2 makes:
5 processing are established in test altogether, 4 sub-districts, and each sub-district area 16m2 carries out experimental scheme, wherein A according to the completely random design: the clear water blank; B:70% is for 1500 times of mancozeb wettable powders; C: 600 times of dilutions of botanical pesticide of the present invention; D: 500 times of dilutions of botanical pesticide of the present invention.E: 400 times of dilutions of botanical pesticide of the present invention.Spray medicine 2 times, interval 7d, 7d investigation preventive effect after the dispenser the last time, 5 points are got in every sub-district, 15 every totally 75 fruits account for the fruit area by lesion area and divide 5 grades of records, refer to the Growth Rate Calculation preventive effect with disease.
Disease index (%)=[∑ (sick fruit number * morbidity progression at different levels)/total fruit of investigation number * superlative degree value] * 100
Relative control effect (%)=[(check plot disease index-treatment region disease index)/check plot disease index] * 100
Table 2 botanical pesticide control of the present invention cucumber downy mildew field test results
Result of the test shows that difference is extremely remarkable between botanical pesticide control cucumber downy mildew of the present invention and the clear water blank; The difference on effect of 500 times and 400 times dilution control cucumber downy mildews of botanical pesticide concentration of the present invention and compound carbendazim is not remarkable; The difference on effect of 600 times of dilution control cucumber downy mildews of botanical pesticide of the present invention and compound carbendazim is remarkable.The concentration of taking all factors into consideration botanical pesticide control cucumber downy mildew of the present invention is the best with 500 times of dilutions, and the control cucumber downy mildew is had positive effect.
Embodiment 6
The botanical pesticide laboratory bacteriostasis test that the embodiment of the invention 1 makes:
With botanical pesticide of the present invention with melted and the cultivation that is chilled to about 60 ℃ is shaken in the culture dish all based on sterilization, make the flat board that contains different pharmaceutical concentration, draw 0.1ml test organisms suspension and on flat board, be coated with evenly; Be coated with test strain as blank on the common not dosing culture medium flat plate; 28 ℃ of constant temperature culture 3d.Do not have lowest concentration of drug in the medicine plate of bacteria growing, be the minimum inhibitory concentration (MIC) of this medicine this bacterium.
Table 3 botanical pesticide laboratory bacteriostasis test of the present invention result (unit: mg/mL)
Result of the test shows that botanical pesticide of the present invention all has more intense bacteriostasis to cereal crop pathogen, tomato early blight bacterium, tomato late blight bacterium, cucumber bacterial angular leaf spot bacterium, green pepper wilt.
Claims (3)
1. botanical pesticide is characterized in that making by the following method:
Raw materials by weight meter: cloves 30-50%, Chinese cassia tree 25-45%, rheum officinale 25-40%;
Preparation process comprises:
1) takes by weighing above-mentioned raw materials, in proportion, pulverize, mix;
2), compound is added the 70-90% alcohol immersion 60-120min of 6-8 times of weight, be heated to 80-85 ℃ and return and heat up in a steamer 30-40min, filter, collect extract; Filter residue adds the 70-90% ethanol of 3-4 times of weight again, is heated to 80-85 ℃ time and heats up in a steamer 20-30min, filters, and collects extract, extracted twice liquid is merged obtain botanical pesticide ethanol extract just;
3), with botanical pesticide ethanol just extract obtain the botanical pesticide ethanol extract 60-65 ℃ of weight that is evaporated to compound;
4), with botanical pesticide ethanol extract and emulsifier by weight 1: 0.05-0.2 mixed product.
2. botanical pesticide as claimed in claim 1 is characterized in that described emulsifier is Tween-80 or alkylphenol polyoxyethylene class emulsifier.
3. botanical pesticide as claimed in claim 1, the application in control cucumber fusarium axysporum, cucumber downy mildew, cucumber bacterial angular leaf spot, green pepper fusarium wilt, early blight of tomato, tomato late blight or cereal crop disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102592585A CN101911956B (en) | 2010-08-18 | 2010-08-18 | Plant source pesticide and production method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102592585A CN101911956B (en) | 2010-08-18 | 2010-08-18 | Plant source pesticide and production method and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN101911956A true CN101911956A (en) | 2010-12-15 |
| CN101911956B CN101911956B (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010102592585A Expired - Fee Related CN101911956B (en) | 2010-08-18 | 2010-08-18 | Plant source pesticide and production method and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349010A (en) * | 2012-04-12 | 2013-10-16 | 南通市双隆农业发展有限公司 | Pesticide for controlling fusarium wilt of cucumber |
| CN104872204A (en) * | 2015-06-17 | 2015-09-02 | 山西农业大学 | Vegetable bactericide capable of preventing and treating phytophthora root rot of vegetables and preparation method of vegetable bactericide |
| CN105309504A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for gray mold disease |
| CN105309505A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical bactericide used for downy mildew |
| CN105309509A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for early blight |
| CN105519599A (en) * | 2015-12-24 | 2016-04-27 | 青岛百瑞吉生物工程有限公司 | Natural bactericide for preventing and treating angular leaf spot of persimmon trees, and preparation method thereof |
| CN106818931A (en) * | 2017-02-15 | 2017-06-13 | 魏代国 | The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse |
| CN108094449A (en) * | 2018-01-15 | 2018-06-01 | 六安职业技术学院 | A kind of botanical pesticide and preparation method and application for preventing Three Akebia Decne Species disease |
| CN111631232A (en) * | 2020-07-07 | 2020-09-08 | 南京慧博生物科技有限公司 | Plant bactericide containing coptis chinensis and rheum officinale and preparation method thereof |
-
2010
- 2010-08-18 CN CN2010102592585A patent/CN101911956B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 张应烙等: "孜然提取物对几种病菌生物活性的初步研究", 《西北农林科技大学学报(自然科学版)》 * |
| 朱立成等: "丁香等3种中草药提取物抑制植物病原真菌的研究", 《安徽农业科学》 * |
| 汪生等: "植物源真菌抑制剂的筛选和复配研究", 《食品科学》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349010A (en) * | 2012-04-12 | 2013-10-16 | 南通市双隆农业发展有限公司 | Pesticide for controlling fusarium wilt of cucumber |
| CN104872204A (en) * | 2015-06-17 | 2015-09-02 | 山西农业大学 | Vegetable bactericide capable of preventing and treating phytophthora root rot of vegetables and preparation method of vegetable bactericide |
| CN105309504A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for gray mold disease |
| CN105309505A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical bactericide used for downy mildew |
| CN105309509A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for early blight |
| CN105519599A (en) * | 2015-12-24 | 2016-04-27 | 青岛百瑞吉生物工程有限公司 | Natural bactericide for preventing and treating angular leaf spot of persimmon trees, and preparation method thereof |
| CN106818931A (en) * | 2017-02-15 | 2017-06-13 | 魏代国 | The biological pesticide of preventing and treating cucumber bacterial angular leaf spot in a kind of day-light greenhouse |
| CN108094449A (en) * | 2018-01-15 | 2018-06-01 | 六安职业技术学院 | A kind of botanical pesticide and preparation method and application for preventing Three Akebia Decne Species disease |
| CN111631232A (en) * | 2020-07-07 | 2020-09-08 | 南京慧博生物科技有限公司 | Plant bactericide containing coptis chinensis and rheum officinale and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN101911956B (en) | 2013-04-24 |
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