CN106172503A - A kind of bacterium drug composition, preparation and application thereof containing dithiocarbamate antibacterial and biocontrol microorganisms - Google Patents
A kind of bacterium drug composition, preparation and application thereof containing dithiocarbamate antibacterial and biocontrol microorganisms Download PDFInfo
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- CN106172503A CN106172503A CN201610509318.1A CN201610509318A CN106172503A CN 106172503 A CN106172503 A CN 106172503A CN 201610509318 A CN201610509318 A CN 201610509318A CN 106172503 A CN106172503 A CN 106172503A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/26—Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides
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Abstract
The invention discloses a kind of bacterium drug composition, preparation and application thereof containing dithiocarbamate antibacterial and biocontrol microorganisms, belong to plant protection and Pesticide Science field.Bacterium drug composition is made up of the dithiocarbamate antibacterial that mass ratio is 1～10:1～10 and biocontrol microorganisms mycopowder, and in mycopowder, viable count is not less than 106cfu/g；Wherein biocontrol microorganisms is preferably bacillus amyloliquefaciens ZY 9 13, deposit number: CGMCC No.6162.Dithiocarbamate antibacterial is multiaction site inhibitor, and it is not likely to produce resistance, with the use of to tobacco root-knot, black shank, there is excellent prophylactic-therapeutic effect with bacillus amyloliquefaciens, preventive effect is significantly better than the single dithiocarbamate antibacterial of use or bacillus amyloliquefaciens biocontrol agent, and the drug-fast generation of chemical agent can be delayed, it is particularly well-suited to the preventing and treating of field tobacco balck shank.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing dithiocarbamate antibacterial and biocontrol microorganisms, also simultaneously
Relate to preparation and the application thereof comprising this bacterium drug composition, belong to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, during wherein the pesticide such as metalaxyl, Mancozeb is production
Use relatively broad medicament.Metalaxyl is benzamides systemic fungicide, and the mid-80 is at China's production and application, at ovum
Playing an important role in fungus diseases preventing and treating, its mechanism of action is polymerase activity in suppression pathogen, and RNA interfering synthesizes.But
Along with produce in commonly used, its resistance problem occurs the most therewith.Chemical agent is compounding can reduce a kind of microbiocidal composition
Consumption, delay drug-fast generation, reduce production cost simultaneously, be one of important measures in control of plant disease.Such as public affairs
A kind of antibacterial for preventing and treating Nicotiana tabacum L. root-rot balck shank disclosed in the patent of invention of cloth CN105265448A, mainly by first frost
Spirit and hymexazol form, and be watered and use after being aided with methanol, emulsifying agent；The patent of invention of publication No. CN104430438A is open
A kind of bactericidal composition, its effective ingredient is cycloheximide triazole and cymoxanil, and the weight ratio of the two is 80:1～1:80, it is adaptable to
The plant disease that preventing and treating is caused by pathogenic fungi, the especially downy mildew on the crop such as black shank and melon fruits and vegetables, frost epidemic disease
Sick etc.；The patent of invention of publication No. CN103478163A discloses a kind of bactericidal pesticide composition containing benzene metsulfovax, except benzene thiophene
Also including AL-PHOSETHYL aluminum tris(ethyl phosphite) outside bacterium amine, the weight ratio of the two is 1:40～60:1, and said composition can be used for preventing and treating black shank, crop
Epidemic disease and downy mildew etc..And above-mentioned patent relates generally to the compounding of chemical agent or medicament development, biological and environmental safety is relatively
Low.
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide the combination of a kind of bacterium medicine containing dithiocarbamate antibacterial and biocontrol microorganisms
Thing, said composition has the prophylactic-therapeutic effect of excellence to tobacco root-knot, black shank etc., and it is single that preventive effect is significantly better than use
The dithiocarbamate antibacterial of one or biocontrol microorganisms microbial inoculum, and the drug-fast generation of chemical agent can be delayed.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is made up of dithiocarbamate antibacterial and biocontrol microorganisms mycopowder, by quality ratio, and two sulfur
For carbamate fungicide: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Described dithiocarbamate antibacterial selected from zineb, ambam, maneb, Carbatene, Mancozeb,
One or more in propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc..As individually selected zineb, Dai Sen
MnZn, Propineb or thiram, or select the combination of maneb and Propineb, propineb and thiram, also or choosing
With zineb, Propineb and the combination of ziram.Compare the single a kind of antibacterial of use, bactericide compounded (two kinds and above kill
Microbial inoculum with the use of) there is summation action or potentiation.Further, composite bactericide and biocontrol microorganisms are used in combination Nicotiana tabacum L. root knot
The prevention effect of nematicide, black shank etc. is better than being used alone chemical bactericide or biocontrol microorganisms microbial inoculum.Meanwhile, compound sterilizing
In agent, each component proportion can arbitrarily be chosen, as Mancozeb compounds according to mass ratio 1～10:1～10 with Propineb, and and for example generation
Gloomy zinc compounds according to mass ratio 1～10:1～10:1～10 with propineb, ziram, etc..Owing to combining form is various,
Do not enumerate.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Wherein, biocontrol microorganisms is excellent
Elect bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13 as, deposit number: CGMCC No.6162,
The preservation time: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Unit address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can
Suppression tobacco black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of anti-root knot of holding concurrently
The Nicotiana tabacum L. endophyte of nematicide and Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, dithio ammonia
Carbamate series bactericidal agent is faint to the growth effect of bacillus amyloliquefaciens (CGMCC No.6162), therebetween compatible
Property good, bacterium medicine combination can be carried out.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is by dithiocarbamate antibacterial and bacillus amyloliquefaciens (CGMCC
No.6162) mycopowder composition, wherein dithiocarbamate antibacterial is multiaction site inhibitor, and is not likely to produce anti-
Property, with the use of to tobacco root-knot, black shank, there is excellent prophylactic-therapeutic effect, preventive effect with bacillus amyloliquefaciens
It is significantly better than the single dithiocarbamate antibacterial of use or bacillus amyloliquefaciens biocontrol agent, and can delaying
Learn the drug-fast generation of medicament, be particularly well-suited to the preventing and treating of field tobacco balck shank.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of zineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, zineb: bacillus amyloliquefaciens mycopowder=3:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with quality
Than meter, propineb: bacillus amyloliquefaciens mycopowder=1:5, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 3
Bacterium drug composition, is made up of Propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, Propineb: bacillus amyloliquefaciens mycopowder=1:10, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 4
Bacterium drug composition, is made up of thiram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, thiram: bacillus amyloliquefaciens mycopowder=1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 5
Bacterium drug composition, by maneb, zineb and and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder form,
By quality ratio, maneb: zineb: bacillus amyloliquefaciens mycopowder=3:3:1, in mycopowder, viable count is not less than 106cfu/
g。
Embodiment 6
Bacterium drug composition, by zineb, Propineb, thiram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder
Composition, by quality ratio, zineb: Propineb: thiram: bacillus amyloliquefaciens mycopowder=3:4:3:1, viable count in mycopowder
It is not less than 106cfu/g。
Embodiment 7
Bacterium medicine preparation, is made up of the raw material of following mass fraction: maneb 25 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 15 parts6Cfu/g), calcium lignosulfonate 5 parts, sodium lauryl sulphate 3
Part, sucrose 2 parts, white carbon 10 parts；Preparation method is: accurately takes each raw material, mix homogeneously according to mass fraction, obtains wettability
Powder.
Embodiment 8
Bacterium medicine preparation, is made up of the raw material of following mass fraction: thiram 6 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 30 parts6Cfu/g), dodecyl sodium sulfate 8 parts, Laurel alcohol radical sodium sulfate
6 parts, sucrose 10 parts, 15 parts of kieselguhr；Preparation method is with embodiment 7.
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: zineb 25 parts, thiram 8 parts, bacillus amyloliquefaciens
(in mycopowder, viable count is not less than 10 to (CGMCC No.6162) mycopowder 10 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 3 parts, pulls open
5 parts of powder, starch 3 parts, 5 parts of sodium sulfate, xanthan gum 2 parts, sucrose 8 parts, bentonite 15 parts, 18 parts of kieselguhr；Preparation method is: press
Accurately take each raw material, pelletize after mix homogeneously according to mass fraction, be dried, sieve, obtain suspending agent.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Mancozeb 15 parts, propineb 5 parts, thiram 20
Part, (in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 8 parts6Cfu/g), dodecyl
Sodium naphthalene sulfonate 6 parts, pulls open 3 parts of powder, 8 parts of sodium sulfate, xanthan gum 7 parts, sucrose 3 parts, Kaolin 25 parts；The same embodiment of preparation method
9。
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: propineb 5 parts, ziram 5 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 25 parts6Cfu/g), sodium lignin sulfonate 8 parts, two fourths
Base sodium naphthalene sulfonate 5 parts, dodecylbenzene sodium sulfonate 6 parts, polyvinylpyrrolidone 5 parts, starch 5 parts, sucrose 10 parts, kieselguhr 20
Part；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtains water-dispersible
Granule.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Mancozeb 20 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 25 parts6Cfu/g), sodium lignin sulfonate 3 parts, nekal 5
Part, dodecylbenzene sodium sulfonate 8 parts, polyvinylpyrrolidone 10 parts, sucrose 8 parts, Kaolin 25 parts；The same embodiment of preparation method
11。
In the various embodiments described above, bacillus amyloliquefaciens (CGMCC No.6162) mycopowder is with reference to patent (notification number
CN102925393B) in detailed description of the invention, method is prepared as wettable powder.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can be by other any two kinds and above bactericide compounded rear and biocontrol microorganisms
Mycopowder combines or makes bacterium medicine preparation.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In process group, bactericide original drug (not adding bacillus amyloliquefaciens) is 10.00 μ g/mL for examination concentration, solves
The concentration of bacillus amyloliquefaciens (CGMCC No.6162) bacteria suspension is 1010Cfu/mL, bacterium drug composition (bactericide original drug 10.00
μ g/mL+ bacillus amyloliquefaciens (CGMCC No.6162) 1010Cfu/mL), compounding form see table 1.Diameter 5mm bacterium dish is put
In the oat medium central authorities of pastille, every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation method to measure
Colony diameter, calculates the different disposal suppression ratio to tobacco black shank bacterium, evaluates bacterium drug composition and makees the suppression of black shank bacterium
With, result see table 1.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Tobacco black shank bacterium mycelial growth is pressed down by table 1 dithiocarbamate antibacterial with bacillus amyloliquefaciens
Effect processed
As it can be seen from table 1 compare chemical bactericide or bacillus amyloliquefaciens, the suppression of tobacco black shank bacterium is imitated
Really, bacterium drug composition shows synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculating tobacco black shank bacterium by greenhouse, and spray bacterium medicine preparation prepared by embodiment 7～12, concrete grammar is such as
Under: the method with reference to Dong Yu prepares bacterium paddy inoculum, is added by the bacterium cake of 20 diameter 5mm in the millet of 500g sterilizing, 28 DEG C
Cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. the Chinese Academy of Agricultural Sciences,
2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum, treats that tobacco seedlings is long
During to 6～7 true leaves, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, and each process 5 strain tobacco seedlings, not apply
Medicament, add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium paddy 10g/ in cigarette strain root after dispenser 3d
Strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 2.In table 2,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., and 50% thiram can
WP is purchased from Shanxi Ke Li Science and Technology Ltd., and bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum is (public with reference to patent
Announcement CN102925393B) prepared by method in detailed description of the invention, is more than matched group.
The table 2 bacterium medicine preparation prevention effect to black shank
From table 2 it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 7～12,
Preventive effect reaches more than 75%, is better than commercially available chemical agent and bacillus amyloliquefaciens microbial inoculum, and the preventing and treating for balck shank provides newly
Type alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (7)
1. bacterium drug composition, it is characterised in that: it is made up of aminodithioformic acid lipid antibacterial and biocontrol microorganisms mycopowder, with quality
Than meter, dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than
106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described dithiocarbamate antibacterial selects
From zineb, ambam, maneb, Carbatene, Mancozeb, propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram
In one or more.
Bacterium drug composition the most according to claim 1 and 2, it is characterised in that: described biocontrol microorganisms is bacillus amyloliquefaciens
ZY-9-13, deposit number: CGMCC No.6162.
4. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of claims 1 to 3.
Bacterium medicine preparation the most according to claim 4, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
6. as according to any one of claims 1 to 3 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
7. bacterium medicine preparation application in preventing and treating root knot nematode in tobacco and/or black shank as described in claim 4 or 5.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109077067A (en) * | 2018-08-16 | 2018-12-25 | 南京农业大学 | A kind of biocontrol microorganisms and its application in terms of crops gray mold prevention and treatment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1695457A (en) * | 2005-03-17 | 2005-11-16 | 中国农业大学 | Mixture of bacterial medicine for controlling vegetable epidemic disease and preparation method |
| CN1930991A (en) * | 2006-10-01 | 2007-03-21 | 黄高 | Re-compounded germicide containing Bucillus licheniformis |
| CN101601411A (en) * | 2008-06-12 | 2009-12-16 | 黄高 | The bactericide that compositely prepared by bacillus licheniformis and chemical bactericide are composite |
| CN101904341A (en) * | 2009-06-04 | 2010-12-08 | 天津市植物保护研究所 | Bio-control bacteria B579 and bactericide synergetic application technology |
| CN102742603A (en) * | 2012-05-31 | 2012-10-24 | 青岛农业大学 | Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide |
| CN102925393A (en) * | 2012-11-07 | 2013-02-13 | 中国烟草总公司郑州烟草研究院 | Tobacco endophyte resisting meloidogyne and tobacco phytophthora nicotianae and application thereof |
| CN104531574A (en) * | 2014-12-17 | 2015-04-22 | 安徽科技学院 | Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition |
| CN105494446A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Biological compound seed coating agent and application |
-
2016
- 2016-06-27 CN CN201610509318.1A patent/CN106172503A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1695457A (en) * | 2005-03-17 | 2005-11-16 | 中国农业大学 | Mixture of bacterial medicine for controlling vegetable epidemic disease and preparation method |
| CN1930991A (en) * | 2006-10-01 | 2007-03-21 | 黄高 | Re-compounded germicide containing Bucillus licheniformis |
| CN101601411A (en) * | 2008-06-12 | 2009-12-16 | 黄高 | The bactericide that compositely prepared by bacillus licheniformis and chemical bactericide are composite |
| CN101904341A (en) * | 2009-06-04 | 2010-12-08 | 天津市植物保护研究所 | Bio-control bacteria B579 and bactericide synergetic application technology |
| CN102742603A (en) * | 2012-05-31 | 2012-10-24 | 青岛农业大学 | Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide |
| CN102925393A (en) * | 2012-11-07 | 2013-02-13 | 中国烟草总公司郑州烟草研究院 | Tobacco endophyte resisting meloidogyne and tobacco phytophthora nicotianae and application thereof |
| CN104531574A (en) * | 2014-12-17 | 2015-04-22 | 安徽科技学院 | Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition |
| CN105494446A (en) * | 2015-12-21 | 2016-04-20 | 云南农业大学 | Biological compound seed coating agent and application |
Non-Patent Citations (4)
| Title |
|---|
| 曾衡等: "烟草黑胫病生防菌的筛选、定殖及应用评价", 《应用环境生物学报》 * |
| 李和平等: "《精细化工工艺学》", 31 August 1997 * |
| 杨兵等: "微生物防治烟草黑胫病的研究进展", 《农药》 * |
| 汪汉成等: "烟草黑胫病化学防治的历史与现状", 《中国烟草学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109077067A (en) * | 2018-08-16 | 2018-12-25 | 南京农业大学 | A kind of biocontrol microorganisms and its application in terms of crops gray mold prevention and treatment |
| CN109077067B (en) * | 2018-08-16 | 2021-04-30 | 南京农业大学 | Biocontrol bacterium and application thereof in prevention and control of gray mold of crops |
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Application publication date: 20161207 |