CN106135288A - Bacterium drug composition, preparation and application containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms - Google Patents
Bacterium drug composition, preparation and application containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms Download PDFInfo
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- CN106135288A CN106135288A CN201610509285.0A CN201610509285A CN106135288A CN 106135288 A CN106135288 A CN 106135288A CN 201610509285 A CN201610509285 A CN 201610509285A CN 106135288 A CN106135288 A CN 106135288A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/26—Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides
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Abstract
The invention discloses a kind of bacterium drug composition, preparation and application containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms, belong to plant protection and Pesticide Science field.This bacterium drug composition is made up of Benzoylamide series bactericidal agent, dithiocarbamate antibacterial and biocontrol microorganisms mycopowder, by quality ratio, Benzoylamide series bactericidal agent: dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1～10:1～10:1～10, in mycopowder, viable count is not less than 106cfu/g.Biocontrol microorganisms is preferably bacillus amyloliquefaciens ZY 9 13, deposit number: CGMCC No.6162.Wherein, the fluopicolide of benzamides, zoxamide can be assembled reached bactericidal effect by the spectrin and suppression mitochondrion β tubulin acting on cell membrane respectively, dithiocarbamate antibacterial is multiaction site inhibitor, and this two series bactericidal agent has excellent prevention effect with bacillus amyloliquefaciens with the use of to tobacco root-knot, black shank.
Description
Technical field
The present invention relates to the combination of a kind of bacterium medicine containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms
Thing, also relates to comprise preparation and the application of this bacterium drug composition, belongs to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, wherein the pesticide such as metalaxyl, fluopicolide, Mancozeb
It is that production uses relatively broad medicament.Metalaxyl is phenylamide systemic fungicide, and the mid-80 is raw in China
Producing and use, play an important role in oomycetes disease control, its mechanism of action is the polymerase activity of suppression pathogen, interference
RNA synthesizes.Fluopicolide is the Benzoylamide series bactericidal agent researched and developed by Beyer Co., Ltd, efficient to cause of disease oomycetes, by acting on
The spectrin of cell membrane and reach bactericidal effect, the mechanism of action of its uniqueness makes itself and various sterilization agent equal no interactions Drug resistance
(roc. fluopicolide and the co-toxicity of pyraclostrobin Phytophthora infestans and mixture preparation research [D]. Hebei agriculture
Sparetime university is learned, and 2013).But be as in producing is commonly used, and the resistance problem of chemical bactericide occurs the most therewith.Wang Wanneng etc.
Research shows, after Chemical treatment, the mycelia physical ability of black shank causes the change of mating type, and then it is prominent to develop immunity to drugs
The Drug resistance becoming bacterial strain, even some bacterial strain can stablize heredity, wherein with metalaxyl for representing phenyl amide series bactericidal agent
Be prominent, and resistance level the highest (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. wide
Western agricultural sciences, 2003, (2): 42-44).And the compounding consumption that can reduce a kind of microbiocidal composition of chemical agent, delay to resist
The generation of the property of medicine, reduces production cost simultaneously, is one of important measures in control of plant disease.
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant at present
Report and patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, balck shank
Antibacterial, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, the two
Weight ratio be 80:1～1:80, it is adaptable to the plant disease that caused by pathogenic fungi of preventing and treating, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of thiophene bacterium Han benzene
The bactericidal pesticide composition of amine, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, said composition
Can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide a kind of containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms
Bacterium drug composition, said composition has the prevention effect of excellence to tobacco root-knot, black shank etc., and preventive effect is the most excellent
In using the antibacterial of single kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and
Chemical drug-fast generation can be delayed.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, by Benzoylamide series bactericidal agent, dithiocarbamate antibacterial and biocontrol microorganisms mycopowder group
Become, by quality ratio, Benzoylamide series bactericidal agent: dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1～10:1～
10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Described Benzoylamide series bactericidal agent any one or more in fluopicolide, zoxamide etc..As individually
Select fluopicolide or zoxamide, or select the combination of fluopicolide and zoxamide.Compare the single a kind of benzene first of use
Acid amide fungicides, bactericide compounded (two kinds and above antibacterial with the use of) have summation action or potentiation.Further,
In composite bactericide, each component proportion can arbitrarily be chosen, if fluopicolide and zoxamide are according to mass ratio 1～10:1～10
Combination, etc..
Described dithiocarbamate antibacterial selected from zineb, ambam, maneb, Carbatene, Mancozeb,
One or more in propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc..As individually selected zineb, Dai Sen
MnZn, Propineb or thiram, or select the combination of maneb and Propineb, propineb and thiram, also or choosing
With zineb, Propineb and the combination of ziram.Compare the single a kind of dithiocarbamate antibacterial of use, antibacterial
Compounding (two kinds and above antibacterial with the use of) have summation action or potentiation.Further, composite bactericide and biocontrol microorganisms
Prevention effect to tobacco root-knot, black shank etc. is used in combination be better than being used alone chemical bactericide or biocontrol microorganisms
Microbial inoculum.Meanwhile, in composite bactericide, each component proportion can arbitrarily be chosen, as Mancozeb and Propineb according to mass ratio 1～
10:1～10 compounds, and and for example zineb compounds according to mass ratio 1～10:1～10:1～10 with propineb, ziram, etc.
Deng.Owing to combining form is various, do not enumerate.
Preferably, any one in fluopicolide, the zoxamide etc. of Benzoylamide series bactericidal agent in bacterium drug composition
Kind, dithiocarbamate antibacterial is selected from zineb, ambam, maneb, Carbatene, Mancozeb, methyl for gloomy
Any one in zinc, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc..Fluopicolide, benzoyl in Benzoylamide series bactericidal agent
The sterilization mechanism of bacterium amine etc. is same or similar, and in like manner dithiocarbamate antibacterial, therefore from above-mentioned two series bactericidal agents
Middle choosing any one kind of them compounding respectively, composite bactericide is used in combination with biocontrol microorganisms again, can not only simplify formula, simultaneously to Nicotiana tabacum L. root knot
Nematicide, black shank etc. also can reach good prevention effect.In Benzoylamide series bactericidal agent, the sterilization of fluopicolide
Mechanism is entirely different with current all known Oomycete antibacterial, mainly acts on cell membrane and intercellular specific proteins
And showing bactericidal activity, the thin layer penetrance of its uniqueness can strengthen the lateral conductivity of medicament and longitudinally fed power, to pathogen
Each Main Morphology all have a good inhibitory activity, treatment potential highlights；Zoxamide by suppression pathogen mitochondrion β-
Tubulin assembles and plays bactericidal activity.Dithiocarbamate antibacterial is multiaction site inhibitor, and is not likely to produce
Resistance.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Biocontrol microorganisms is preferably and solves
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13, deposit number: CGMCC No.6162, during preservation
Between: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, unit ground
Location: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can suppress cigarette
Grass black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of hold concurrently anti-root-knot nematode and
The Nicotiana tabacum L. endophyte of Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, benzamides sterilizes
Agent, dithiocarbamate antibacterial are faint to the growth effect of bacillus amyloliquefaciens (CGMCC No.6162), sterilization
The compatibility between agent and bacterial strain is good, can carry out bacterium medicine combination.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is conciliate starch by Benzoylamide series bactericidal agent, dithiocarbamate antibacterial
Bacillus cereus (CGMCC No.6162) mycopowder forms, and wherein the fluopicolide of benzamides is by acting on the blood of cell membrane
Shadow albumen reaches bactericidal effect, and zoxamide is assembled by suppression pathogen mitochondrion β-tubulin and plays bactericidal activity, two sulfur
It is multiaction site inhibitor for carbamate fungicide, and is not likely to produce resistance, this two series bactericidal agent and solution starch bud
Spore bacillus has excellent prevention effect with the use of to tobacco root-knot, black shank, and preventive effect is significantly better than use
The antibacterial of single kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and can delay
The drug-fast generation of chemistry, is particularly well-suited to the preventing and treating of field tobacco balck shank.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of fluopicolide, zineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, fluopicolide: zineb: bacillus amyloliquefaciens mycopowder=1:1:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of zoxamide, zineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, zoxamide: zineb: bacillus amyloliquefaciens mycopowder=5:2:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 3
Bacterium drug composition, by fluopicolide, propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, fluopicolide: propineb: bacillus amyloliquefaciens mycopowder=10:5:7, in mycopowder, viable count is the lowest
In 106cfu/g。
Embodiment 4
Bacterium drug composition, is made up of fluopicolide, Propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, fluopicolide: Propineb: bacillus amyloliquefaciens mycopowder=10:10:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 5
Bacterium drug composition, is made up of zoxamide, thiram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, zoxamide: thiram: bacillus amyloliquefaciens mycopowder=2:1:10, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 6
Bacterium drug composition, by zoxamide, Mancozeb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, zoxamide: Mancozeb: bacillus amyloliquefaciens mycopowder=2:10:7, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 7
Bacterium drug composition, is made up of fluopicolide, ziram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, fluopicolide: ziram: bacillus amyloliquefaciens mycopowder=5:5:3, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 8
Bacterium drug composition, by fluopicolide, zoxamide, Mancozeb and bacillus amyloliquefaciens (CGMCC
No.6162) mycopowder composition, by quality ratio, fluopicolide: zoxamide: Mancozeb: bacillus amyloliquefaciens mycopowder=3:
6:7:9, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 24 parts, thiram 32 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 4 parts6Cfu/g), dodecyl sodium sulfate 6 parts, Laurel
3 parts of alcohol radical sodium sulfate, sodium lauryl sulphate 5 parts, ethyl cellulose 3 parts, sucrose 5 parts, 18 parts of kieselguhr, attapulgite 14
Part；Preparation method is: accurately takes each raw material, grinding and sieving, mix homogeneously according to mass fraction, obtains wettable powder.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: zoxamide 10 parts, zineb 35 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 10 parts6Cfu/g), calcium lignosulfonate 10 parts, 12
Alkyl sodium sulfate 5 parts, sucrose 3 parts, white carbon 14 parts, 15 parts of kieselguhr；Preparation method is with embodiment 9.
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 15 parts, propineb 15 parts, solves starch
(in mycopowder, viable count is not less than 10 to bacillus cereus (CGMCC No.6162) mycopowder 35 parts6Cfu/g), dodecyl sodium sulfate 12
Part, 6 parts of Laurel alcohol radical sodium sulfate, calcium lignosulfonate 5 parts, ethyl cellulose 5 parts, sucrose 5 parts, Kaolin 20 parts；Preparation side
Method is with embodiment 9.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 18 parts, Mancozeb 18 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 18 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 6
Part, pull open 5 parts of powder, 2 parts of sodium sulfate, xanthan gum 8 parts, sucrose 4 parts, Kaolin 12 parts；Preparation method is: accurate according to mass fraction
Really take each raw material, pelletize after mix homogeneously, it is dried, sieves, obtain suspending agent.
Embodiment 13
Bacterium medicine preparation, is made up of the raw material of following mass fraction: zoxamide 5 parts, thiram 6 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 47 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 10 parts, draws
Open 3 parts of powder, starch 4 parts, 3 parts of sodium sulfate, xanthan gum 5 parts, sucrose 5 parts, bentonite 17 parts, 12 parts of kieselguhr；Preparation method is same
Embodiment 12.
Embodiment 14
Bacterium medicine preparation, is made up of the raw material of following mass fraction: zoxamide 22 parts, ziram 15 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 38 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 5 parts,
Pull open 5 parts of powder, 3 parts of sodium sulfate, sucrose 5 parts, bentonite 15 parts, 15 parts of kieselguhr；Preparation method is with embodiment 12.
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 40 parts, Propineb 5 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 5 parts6Cfu/g), polyvinylpyrrolidone 5 parts, dodecane
Base phenol polyethenoxy ether 3 parts, dodecylbenzene sodium sulfonate 3 parts, 3 parts of oxirane, ethyl cellulose 10 parts, attapulgite 18
Part；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtains water-dispersible
Granule.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 10 parts, zoxamide 10 parts, Mancozeb
10 parts, thiram 10 parts, (in mycopowder, viable count is not less than bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 10 parts
106Cfu/g), sodium lignin sulfonate 8 parts, nekal 3 parts, dodecylbenzene sodium sulfonate 3 parts, polyvinylpyrrolidine
Ketone 5 parts, starch 5 parts, sucrose 3 parts, 15 parts of kieselguhr；Preparation method is with embodiment 15.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can be from Benzoylamide series bactericidal agent, dithiocarbamate sterilization
In agent optional multiple compounding, composite bactericide again with biocontrol microorganisms combination or make bacterium medicine preparation.
Comparative example 1
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 56 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 4 parts6Cfu/g), other auxiliary agents and preparation method are with embodiment 9.
Comparative example 2
Bacterium medicine preparation, is made up of the raw material of following mass fraction: thiram 56 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 4 parts6Cfu/g), other auxiliary agents and preparation method are with embodiment 9.
Bacillus amyloliquefaciens (CGMCC No.6162) mycopowder in the various embodiments described above and comparative example is (public with reference to patent
Announcement CN102925393B) method is prepared as wettable powder in detailed description of the invention.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In table 1, bactericide original drug (not adding bacillus amyloliquefaciens) is 1.50 μ g/mL for examination concentration, benzene in table 2
Benzamide type bactericide original drug (not adding bacillus amyloliquefaciens) is 0.15 μ g/mL for examination concentration, dithiocarbamates first in table 3
The former medicine of bactericide of phosphate (phosphinium) ester (not adding bacillus amyloliquefaciens) is 10.00 μ g/mL for examination concentration；Bacillus amyloliquefaciens
The concentration of (CGMCC No.6162) bacteria suspension is 1010Cfu/mL, bacterium drug composition (all kinds of bactericide original drug corresponding concentration+Xie Dian
Afnyloliquefaciens (CGMCC No.6162) 1010Cfu/mL), compounding form is specifically shown in table 1 below～3.Diameter 5mm bacterium dish is placed in
The oat medium central authorities of pastille, every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation method to measure bacterium
Fall diameter, calculates the different disposal suppression ratio to tobacco black shank bacterium, evaluates the bacterium drug composition inhibitory action to black shank bacterium,
Result see table 1～3.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Mycelial growth is pressed down by table 1 benzamides, dithiocarbamate antibacterial with bacillus amyloliquefaciens
Effect processed
Table 2 Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Tobacco black shank bacterium mycelial growth is pressed down by table 3 dithiocarbamate antibacterial with bacillus amyloliquefaciens
Effect processed
From table 1～3 it can be seen that conciliate starch containing Benzoylamide series bactericidal agent, dithiocarbamate antibacterial
The bacterium drug composition of bacillus cereus (CGMCC No.6162) is significantly better than the single kind of use to the inhibition of tobacco black shank bacterium
The antibacterial of class, bacillus amyloliquefaciens and any of the above-described kind of series bactericidal agent and the combination of bacillus amyloliquefaciens.Show this
Invention has synergistic function between each component of bacterium drug composition.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculate tobacco black shank bacterium by greenhouse, and spray embodiment 9～16 and the bacterium medicine preparation of comparative example 1～2 preparation,
Concrete grammar is as follows: the method with reference to Dong Yu prepares bacterium paddy inoculum, and the bacterium cake of 20 diameter 5mm adds the paddy of 500g sterilizing
In son, 28 DEG C of cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. China's agriculture
Industry academy of science, 2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum,
When tobacco seedlings length to 6～7 slice true leaf, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, each process 5 strain tobacco seedlings,
Not apply medicament, to add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium in cigarette strain root after dispenser 3d
Paddy 10g/ strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 4.In table 4,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 687.5g/L silver method
Profit (fluorine bacterium Propamocarb) is purchased from Shanxi Ke Li Science and Technology Ltd. purchased from Beyer Co., Ltd, 50% thiram wettable powder, solves
Bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum is with reference to method in patent (notification number CN102925393B) detailed description of the invention
Preparation, is more than matched group.
The table 4 bacterium medicine preparation prevention effect to black shank
From table 4, it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 9～16,
Preventive effect reaches more than 82%, is better than bacterium medicine preparation in commercially available chemical agent, bacillus amyloliquefaciens microbial inoculum and comparative example 1～2, for
The preventing and treating of balck shank provides novel alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (8)
1. bacterium drug composition, it is characterised in that: by Benzoylamide series bactericidal agent, dithiocarbamate antibacterial and biological and ecological methods to prevent plant disease, pests, and erosion
Bacterium mycopowder forms, by quality ratio, and Benzoylamide series bactericidal agent: dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1
～10:1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described Benzoylamide series bactericidal agent is selected from fluorine pyrrole bacterium
One or both in amine, zoxamide.
Bacterium drug composition the most according to claim 1, it is characterised in that: described dithiocarbamate antibacterial selects
From zineb, ambam, maneb, Carbatene, Mancozeb, propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram
In one or more.
4. according to the bacterium drug composition according to any one of claims 1 to 3, it is characterised in that: described biocontrol microorganisms is for solving starch
Bacillus cereus ZY-9-13, deposit number: CGMCC No.6162.
5. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of Claims 1 to 4.
Bacterium medicine preparation the most according to claim 5, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
7. as according to any one of Claims 1 to 4 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
8. the application in preventing and treating root knot nematode in tobacco and/or black shank of the bacterium medicine preparation as described in claim 5 or 6.
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| US20150157026A1 (en) * | 2012-05-30 | 2015-06-11 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the mitosis and cell division or compounds having a multi-site action |
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| CN101653118A (en) * | 2009-07-31 | 2010-02-24 | 深圳诺普信农化股份有限公司 | Compound synergistic bactericidal composition |
| US20150157026A1 (en) * | 2012-05-30 | 2015-06-11 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the mitosis and cell division or compounds having a multi-site action |
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