CN102742603A - Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide - Google Patents
Bactericide used for controlling brassica rapa pekinensis downy mildew and prepared by complexation of bacillus subtilis and chemical bactericide Download PDFInfo
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- CN102742603A CN102742603A CN2012101754191A CN201210175419A CN102742603A CN 102742603 A CN102742603 A CN 102742603A CN 2012101754191 A CN2012101754191 A CN 2012101754191A CN 201210175419 A CN201210175419 A CN 201210175419A CN 102742603 A CN102742603 A CN 102742603A
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Abstract
The invention relates to a pesticide, and especially relates to a complex bactericide composed of bacillus subtilis and a chemical bactericide. The bactericide is used for controlling brassica rapa pekinensis downy mildew. The bactericide comprises the chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, and auxiliary materials. The bactericide is characterized in that the chemical bactericide in the active components is any one selected from metalaxyl, propamocarb, and mancozeb. A biocontrol bacterium is bacillus subtilis XM16, with the strain collection number of CGMCC No.0372. A weight ratio of metalaxyl to bacillus subtilis XM16 is 1-8:1-10. A weight ratio of propamocarb to bacillus subtilis XM16 is 1-10:15-140. A weight ratio of mancozeb to bacillus subtilis XM16 is 1-15:1-10. A form of the bactericide is usually hydrophilic powder or aqueous solution. The complex agent of the biocontrol bacterium and the chemical bactericide has a substantial synergetic effect in controlling brassica rapa pekinensis downy mildew. The biocontrol bacterium bacillus subtilis XM16 is advantaged in high bacteriostatic activity, high stress resistance, and wide bactericidal spectrum. The biocontrol bacterium is complexed with the oomycetes chemical bactericide, such that the pesticide dosage is reduced, and controlling effect is improved. The bactericide is an environment-friendly bactericide. The bactericide is advantaged in high biological safety, low cost, and substantial economic benefit.
Description
Technical field
The present invention relates to composite bactericide,, belong to biocontrol of plant disease and chemical control field, be exclusively used in the integrated control of Chinese cabbage downy mildew through with bacillus subtilis and the bactericide compounded purpose that reaches control Chinese cabbage downy mildew of oomycetes.
Background technology
Chinese cabbage (Brassica rapa pekinensis) is common brassicaceous vegetable; Chinese cabbage downy mildew is a kind of important oomycetes disease that is caused by parasitic downy mildew (Peronospora parasitica), can cause the loss of 20%-60% in the production in the popular time of disease.Being very popular of excessive Chinese cabbage downy mildew takes place in the sixties in 20th century in China, and agricultural production has been caused serious loss.Because downy mildew has a strong impact on the yield and quality of Chinese cabbage, adopt the reinforcement cultivation management in the production usually, select for use measures such as disease-resistant variety, chemical agent control to alleviate its harm.The chemical agent control remains one of main means of control Chinese cabbage downy mildew harm in many prophylactico-therapeutic measuress.
Chemical agent control Chinese cabbage downy mildew; It is fine to the downy mildew control efficiency to spray medicine at the initial stage of a disease; Like (China's Vegetable, 2001, (6): reported the field efficacy of several kinds of main oomycetes bactericide 38-39) such as " 6 kinds of bactericide to the preventive effect of Chinese cabbage downy mildew relatively " Shang Haiqing to Chinese cabbage downy mildew; Find that active ingredient mancozeb, dimethomorph and combination thereof are best to Chinese cabbage downy mildew preventive effect, can reach more than 85%.Yet mancozeb is a kind of protective fungicide, and lower to the virulence of pathogen when control Chinese cabbage downy mildew, if it is higher effectively to prevent and treat the dosage of disease needs, the residue of pesticide problem is serious; And dimethomorph is as novel systemic fungicide, and action site is single, the long-term use, and pathogen is easy to generate pesticide resistance, also is prone to form cross resistance with other medicaments, and the resistance risk level is higher.
Bacillus subtilis (Bacillus subtilis) is a kind of important biocontrol microorganisms; Its main mechanism of action is: it is strong that the competition effect of (1) dominant population---bacillus subtilis has on crop a colonization ability; Breeding is fast; Can infect the site with the pathogen effective competition, reduce pathogen and invade probability.(2) bacteriostasis of antibacterial protein---bacillus subtilis can secrete antibacterial protein, and the spore germination of pathogen, spore growth are had remarkable inhibitory action.And bacillus subtilis is stronger to the tolerance of environment, mix with chemical agent can grow surely on the blade face after spraying expand numerous.
Composite through bacillus subtilis with the chemical bactericide of control Chinese cabbage downy mildew; Can filter out a kind of efficient, low toxicity, eco-friendly composite bactericide; The new medicament that obtains can effectively be prevented and treated Chinese cabbage downy mildew; And significantly reduced the using dosage of this chemical agent list agent, reduced residue of pesticide, the antibacterial protein that produces in the fermentation of bacillus subtilis process simultaneously also can play inhibitory action to other blade face pathogenic microorganisms.
Summary of the invention
The technical problem that the present invention will solve provides a kind of the raising active total amount, the environment amenable composite bactericide that contains bacillus subtilis is used in Chinese cabbage downy mildew control efficiency, reduction.
The present invention solves the problems of the technologies described above with following technical scheme: the composite bactericide of one type of control Chinese cabbage downy mildew of being made up of biocontrol microorganisms and chemical bactericide; It is characterized in that forming by chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, filler, auxiliary agent; Said chemical bactericide is any one in metalaxyl (metalaxyl), Propamocarb (propamocarb), the mancozeb (mancozeb), and the viable count of the former powder of said bacillus subtilis XM16 is 1.05 * 10
12Individual/g, the viable count of fermentation of bacillus subtilis concentrate is 1.0 * 10
11Individual/g.
On the basis of such scheme; Said metalaxyl and bacillus subtilis XM16 weight ratio are 1 ~ 8:1 ~ 10, and metalaxyl (metalaxyl) belongs to the phenyl amide series bactericidal agent, are new and effective systemic fungicides; Can interior inspiration go in the plant; The general bactericide of water-soluble ratio is much higher, can penetrate the little oomycetes cell membrane of lipophilicity, plays bactericidal action.Metalaxyl has the selectivity special efficacy to Peronospora in the Oomycete and Phytophthora fungi; Inhibitory action to release, sprouting and the intrusion of the zoospore of oomycetes is not clearly; But each Main Stage that can suppress disease development behind the germ invaded plants; Like the growth of mycelia in plant corpus, haustorium and sporangial formation have protection and therapeutic action; Propamocarb and bacillus subtilis XM16 weight ratio are 1 ~ 10:15 ~ 140; Propamocarb (propamocarb) belongs to carbamate fungicide; Its mechanism of action is that the phosphoric acid of inhibition germ cell membrane component and the biochemistry of fatty acid synthesize, and suppresses mycelial growth and spreads and sporangial formation and sprouting.Propamocarb is effective especially to Oomycete fungi (like downy mildew); It is a kind of wide-spectrum bactericide; Fungies such as Aphanomyces, Bremia, Peronospora, Phytophthora, false Peronospora, pythium all there is fine killing action; Have local preferably systemic action, also can be used as impregnation process and seed protectant; Mancozeb and bacillus subtilis XM16 weight ratio are 1 ~ 15:1 ~ 10, and mancozeb (mancozeb) belongs to the bactericide of dithiocarbamates, and its bactericidal mechanism is many-sided, are mainly the oxidation that suppresses pyruvic acid in the thalline.Mancozeb is a kind of broad spectrum protection property bactericide; Vaccine to Oomycete belongs to, the Cercospora of Fungi Imperfecti; The various crop disease that Ascochyta etc. cause; Like the leaf spots of vegetables, fruit tree and various crops, blossom rot etc. good control efficiency is arranged all,, vegetable epidemic disease melon to preventing and treating, downy mildew effect are remarkable.
On the basis of such scheme, said auxiliary agent is one or more a mixture of wetting agent, emulsifier, dispersant.
On the basis of such scheme, said wetting agent is a kind of in sodium butylnaphthalenesulfonate, neopelex, aliphatic alcohol polyethenoxy base ether, the alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate.
On the basis of such scheme, said dispersant is one or more a mixture of naphthalene sulfonic acid-formaldehyde condensation product sodium salt, lignin sulfonic acid sodium salt, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate.
On the basis of such scheme, said emulsifier is 0203B.
On the basis of such scheme, said filler is one or more the mixture of precipitated calcium carbonate, white carbon, kaolin, diatom.
On the basis of such scheme, this composite bactericide formulation is wetting powder or aqua.
A kind of composite bactericide wetting powder of preventing and treating Chinese cabbage downy mildew disease contains the aforesaid active component of being made up of phenylamide fungicide metalaxyl and bacillus subtilis, and the weight proportion of concrete component and each component is:
A kind of composite bactericide aqua of preventing and treating Chinese cabbage downy mildew contains the above-mentioned active component of being made up of carbamate fungicide Propamocarb and bacillus subtilis, and the weight proportion of concrete component and each component is:
A kind of composite bactericide wetting powder of preventing and treating Chinese cabbage downy mildew contains the above-mentioned active component of being made up of the bactericide mancozeb and the bacillus subtilis of dithiocarbamates, and the weight proportion of concrete component and each component is:
The former powder of bacillus subtilis is the viable count 1.05 * 10 that contains in every g dry powder in the said active component
12Individual.The fermentation of bacillus subtilis concentrate be contain in every g unit concentrate viable count 1.0 * 10
11Individual.Bacillus subtilis and above-mentioned chemical bactericide are re-dubbed regular dosage form by a certain percentage to be used.
Said wetting agent is a kind of in sodium butylnaphthalenesulfonate, neopelex, aliphatic alcohol polyethenoxy base ether, the alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate; Said dispersant is one or more a mixture of naphthalene sulfonic acid-formaldehyde condensation product sodium salt, lignin sulfonic acid sodium salt, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate; Said filler is one or more the mixture of precipitated calcium carbonate, white carbon, kaolin, diatom; Said emulsifier is 0203B.
The preparation process of the composite bactericide wetting powder of control Chinese cabbage downy mildew is: phenylamide fungicide metalaxyl or dithiocarbamate fungicide mancozeb, the former powder of bacillus subtilis, wetting agent, dispersant and filler are mixed stirring and grind (air-flow) pulverizing according to said ratio, obtain wetting powder.
The preparation process of the composite bactericide aqua of control Chinese cabbage downy mildew is: carbamate fungicide Propamocarb, fermentation of bacillus subtilis concentrate, emulsifier are added water by above-mentioned weight ratio and mix, and stir on mixing limit, limit, obtains aqua.
The composite bactericide that contains bacillus subtilis of the present invention is used to prevent and treat Peronospora (Perononspora) fungi has special efficacy, and its reason is:
1. the chemical bactericide that is utilized to the spore germination of phytopathogen, infect host, mycelial growth, pathogen metabolism etc. remarkable inhibitory action arranged.Cooperate bacillus subtilis that the antibacterial material that the competitiveness of phytopathogen suppresses, secretes is suppressed effects such as pathogen normal growth, composite back forms the synergistic effect of having complementary advantages, thereby reaches the purpose of effective and sustainable Control disease.
2. bacillus subtilis and the above-mentioned various chemical bactericide with preparation carries out composite with arbitrary proportion; As long as choose appropriate compound proportion; Just can make the application of agricultural chemicals of the present invention can bring into play the optimum medicine efficacy effect; Can reduce the usage amount and the access times of the agent of chemical agent list again, reduce harm environment.
3. bacillus subtilis is higher to the poor environment tolerance.There is not any harmful effect in growth to bacillus subtilis behind above-mentioned each composite reagent.
The invention has the beneficial effects as follows: this composite bactericide has special efficacy to control Chinese cabbage downy mildew; The synergistic effect of this composite bactericide is remarkable, bacillus subtilis use the remarkable using dosage that reduces the agent of chemical agent list, reduced residue of pesticide; Alleviated the harm of chemical pesticide to environment; The new medicament that obtains can effectively be prevented and treated Chinese cabbage downy mildew, and the antibacterial protein that produces in the fermentation of bacillus subtilis process simultaneously also can play inhibitory action to other blade face pathogenic microorganisms, and the composite dose of action site to pathogen increased; Can effectively delay drug-fast generation of Chinese cabbage Pseudoperonospora cubensis and development, prolong the service life of new chemical bactericide.The preservation information of bacterial classification of the present invention is following:
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center
(the English CGMCC that is called for short)
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on December 2nd, 1998
Deposit number: CGMCC No.0372
Classification name: bacillus subtilis XM16 Bacillus subtilis XM16
The practical implementation technology
Experimental technique among the following embodiment if no special instructions, is conventional method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
In compound process, described preparation is the preparation method by routine, promptly in composite composition, adds known solvent or carrier, emulsifier and dispersant and processes required formulation.
Embodiment 1 gets former powder 34.01 grams of 98% metalaxyl, the former powder (1.05 * 10 of bacillus subtilis
12Individual/as g) 37.7 to restrain, sodium butylnaphthalenesulfonate 25 grams, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 50 grams and precipitated calcium carbonate 353.29 grams.Mo mixed research, that is made of 6.67% metalaxyl · 7.9 × 10
10 live spores / g Bacillus subtilis WP 500 grams.Metalaxyl (metalaxyl) Nanjing He Yuan chemistry Co., Ltd
Embodiment 2 gets 98% Propamocarb stoste, 15.31 grams, fermentation of bacillus subtilis concentrate (1.0 * 10
11Individual/as g) 335.78 to restrain, emulsifier 0203B 25 grams and water 123.91 grams.Earlier Propamocarb stoste is mixed with the fermentation of bacillus subtilis concentrate, stir, add 0203B 25 grams then, add water again and supply 500 grams, after stirring, promptly process 500 grams, 3% Propamocarb 6.72 * 10 while mixing
10Individual living spores/gram bacillus subtilis aqua.Propamocarb (propamocarb) Zhejiang Heben Science & Technology Co., Ltd.
0203B Shandong natural laws biotechnology Co., Ltd
Embodiment 3 gets 98% Propamocarb stoste, 10.20 grams, fermentation of bacillus subtilis concentrate (1.0 * 10
11Individual/as g) 335.42 to restrain, emulsifier 0203B 25 grams and water 129.38 grams.Earlier Propamocarb stoste is mixed with the fermentation of bacillus subtilis concentrate, stir, add the 0203B25 gram then, add water again and supply 500 grams, after stirring, promptly process 500 grams, 2% Propamocarb 6.72 * 10 while mixing
10Individual living spores/gram bacillus subtilis aqua.
Propamocarb (propamocarb) Zhejiang Heben Science & Technology Co., Ltd.
0203B Shandong natural laws biotechnology Co., Ltd
Embodiment 4 gets 90% mancozeb original powder, 83.34 grams, the former powder (1.05 * 10 of bacillus subtilis
12Individual/as g) 45.45 to restrain, sodium butylnaphthalenesulfonate 25 grams, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 50 grams and precipitated calcium carbonate 296.21 grams.Mo mixed research, that is made of 500 grams of 15% mancozeb · 1.0 × 10
11 live spores / g Bacillus subtilis WP.
Agricultural chemical industry Co., Ltd of the positive nation in mancozeb (mancozeb) Xinyi City
Embodiment 5 gets 90% mancozeb original powder, 37.54 grams, the former powder (1.05 * 10 of bacillus subtilis
12Individual/as g) 47.64 to restrain, sodium butylnaphthalenesulfonate 25 grams, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 50 grams and precipitated calcium carbonate 339.82 grams.Mo mixed research, that is made of 500 grams 6.76% mancozeb · 1.0 × 10
11 live spores / g Bacillus subtilis WP.
Agricultural chemical industry Co., Ltd of the positive nation in mancozeb (mancozeb) Xinyi City
The control efficiency result of the test of preventing and treating Chinese cabbage downy mildew with the goods of embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5 is following:
The goods of table 11 embodiment 1,2,3,4,5 are to the preventive effect of Chinese cabbage downy mildew
Claims (9)
1. the composite bactericide of one type of control Chinese cabbage downy mildew of forming by biocontrol microorganisms and chemical bactericide; It is characterized in that forming by chemical bactericide, bacillus subtilis XM16 CGMCCNo.0372, auxiliary material; Said chemical bactericide is any one in metalaxyl (metalaxyl), Propamocarb (propamocarb), the mancozeb (mancozeb), and the viable count of said bacillus subtilis XM16 is 1.05 * 10
12Individual/g.
2. composite bactericide according to claim 1 is characterized in that said metalaxyl and bacillus subtilis XM16 weight ratio are 1 ~ 8:1 ~ 10; Propamocarb and bacillus subtilis XM16 weight ratio are 1 ~ 10:15 ~ 140; Mancozeb and bacillus subtilis XM16 weight ratio are 1 ~ 15:1 ~ 10.
3. composite bactericide according to claim 1 is characterized in that said auxiliary material is one or both a mixture of filler, auxiliary agent.
4. composite bactericide according to claim 3 is characterized in that said auxiliary agent is one or more a mixture of wetting agent, emulsifier, dispersant.
5. composite bactericide according to claim 4 is characterized in that said wetting agent is a kind of in sodium butylnaphthalenesulfonate, neopelex, aliphatic alcohol polyethenoxy base ether, the alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate.
6. composite bactericide according to claim 4 is characterized in that said dispersant is one or more a mixture of naphthalene sulfonic acid-formaldehyde condensation product sodium salt, lignin sulfonic acid sodium salt, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate.
7. composite bactericide according to claim 3 is characterized in that said filler is one or more the mixture of precipitated calcium carbonate, white carbon, kaolin, diatom.
8. composite bactericide according to claim 4 is characterized in that said emulsifier is 0203B.
9. composite bactericide according to claim 1 is characterized in that this composite bactericide formulation is wetting powder or aqua.
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| CN105994381A (en) * | 2016-06-27 | 2016-10-12 | 中国烟草总公司郑州烟草研究院 | Bactericide composition containing carboxylic acid amide and dithiocarbamate bactericides and biocontrol bacterium, preparation and applications |
| CN106035383A (en) * | 2016-06-27 | 2016-10-26 | 中国烟草总公司郑州烟草研究院 | Bactericide composition containing chemical bactericides and biocontrol bacterium, preparation and application of bactericide composition or preparation |
| CN106106533A (en) * | 2016-06-27 | 2016-11-16 | 中国烟草总公司郑州烟草研究院 | A kind of bacterium drug composition, preparation and application thereof containing acid amide fungicides and biocontrol microorganisms |
| CN106135286A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Composition containing benzamides, dinitroaniline, dithiocarbamate bactericide and biocontrol microorganisms, preparation and application |
| CN106135288A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition, preparation and application containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms |
| CN106135287A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition containing dinitroaniline, dithiocarbamate bactericide and biocontrol microorganisms, preparation and application |
| CN106172503A (en) * | 2016-06-27 | 2016-12-07 | 中国烟草总公司郑州烟草研究院 | A kind of bacterium drug composition, preparation and application thereof containing dithiocarbamate antibacterial and biocontrol microorganisms |
| CN106818881A (en) * | 2016-12-30 | 2017-06-13 | 西北农林科技大学 | A kind of complex preparation of bacillus subtilis and chemical pesticide and preparation method thereof |
| JP2018514513A (en) * | 2015-03-27 | 2018-06-07 | バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ | Methods and compositions for reducing fungal infestation and improving gramineous plant quality |
| CN112195135A (en) * | 2020-11-06 | 2021-01-08 | 青岛农业大学 | Bacillus belgii and application thereof in preventing and treating plant downy mildew disease |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2018514513A (en) * | 2015-03-27 | 2018-06-07 | バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ | Methods and compositions for reducing fungal infestation and improving gramineous plant quality |
| CN105994381A (en) * | 2016-06-27 | 2016-10-12 | 中国烟草总公司郑州烟草研究院 | Bactericide composition containing carboxylic acid amide and dithiocarbamate bactericides and biocontrol bacterium, preparation and applications |
| CN106035383A (en) * | 2016-06-27 | 2016-10-26 | 中国烟草总公司郑州烟草研究院 | Bactericide composition containing chemical bactericides and biocontrol bacterium, preparation and application of bactericide composition or preparation |
| CN106106533A (en) * | 2016-06-27 | 2016-11-16 | 中国烟草总公司郑州烟草研究院 | A kind of bacterium drug composition, preparation and application thereof containing acid amide fungicides and biocontrol microorganisms |
| CN106135286A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Composition containing benzamides, dinitroaniline, dithiocarbamate bactericide and biocontrol microorganisms, preparation and application |
| CN106135288A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition, preparation and application containing benzamides, dithiocarbamate antibacterial and biocontrol microorganisms |
| CN106135287A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition containing dinitroaniline, dithiocarbamate bactericide and biocontrol microorganisms, preparation and application |
| CN106172503A (en) * | 2016-06-27 | 2016-12-07 | 中国烟草总公司郑州烟草研究院 | A kind of bacterium drug composition, preparation and application thereof containing dithiocarbamate antibacterial and biocontrol microorganisms |
| CN106818881A (en) * | 2016-12-30 | 2017-06-13 | 西北农林科技大学 | A kind of complex preparation of bacillus subtilis and chemical pesticide and preparation method thereof |
| CN112195135A (en) * | 2020-11-06 | 2021-01-08 | 青岛农业大学 | Bacillus belgii and application thereof in preventing and treating plant downy mildew disease |
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Application publication date: 20121024 |