CN106106530A - Containing phenylamide, dinitroaniline, dithiocarbamate antibacterial and the compositions of biocontrol microorganisms, preparation and application - Google Patents
Containing phenylamide, dinitroaniline, dithiocarbamate antibacterial and the compositions of biocontrol microorganisms, preparation and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/26—Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides
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Abstract
The invention discloses a kind of containing phenylamide, dinitroaniline, dithiocarbamate antibacterial and the compositions of biocontrol microorganisms, preparation and application, belong to plant protection and Pesticide Science field.Said composition is 1～10:1～10:1 by mass ratio～the phenyl amide series bactericidal agent of 10:1～10, dinitroaniline series bactericidal agent, dithiocarbamate antibacterial and biocontrol microorganisms mycopowder form, and in mycopowder, viable count is not less than 106cfu/g.Biocontrol microorganisms is preferably bacillus amyloliquefaciens ZY 9 13, deposit number: CGMCC No.6162.Phenyl amide series bactericidal agent can suppress the activity of RNA polymerase in pathogen, the fluazinam of dinitroaniline can make the oxidative phosphorylation uncoupling in pathogen Repiration, obstruction energy synthesizes, dithiocarbamate antibacterial is multiaction site inhibitor, this three series bactericidal agent has excellent prevention effect with bacillus amyloliquefaciens with the use of to tobacco root-knot, black shank, and can delay chemical drug-fast generation.
Description
Technical field
The present invention relates to a kind of containing phenylamide, dinitroaniline, dithiocarbamate antibacterial and life
Fungi-proofing compositions, also relates to comprise the preparation of said composition and application, belongs to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, and wherein the pesticide such as metalaxyl, fluazinam, Mancozeb is
Production uses relatively broad medicament.Metalaxyl is phenylamide systemic fungicide, and the mid-80 produces in China
Using, play an important role in oomycetes disease control, its mechanism of action is the polymerase activity of suppression pathogen, interference
RNA synthesizes.Fluazinam (SHIRLAN, IKF-1216) is that 6-dinitroaniline is wide by the 2 of the exploitation of Shi Yuan industry companies of Japan
Spectrum antibacterial, is resisted by suppression Sporulation and sprouting, mycelial growth etc. and infects, and resistance of rainwater washing against, lasting period are long, to ash Portugal
The multiple gray mold that grape spore causes has specially good effect, same to benzimidazole, dicarboximide class and the most existing antibacterial
No interactions resistance, also has germ diseases such as interlink spore genus, Phytophthora, Plasmopara, Sclerotinia and Venturias
Good activity.But be as in producing is commonly used, and the resistance problem of chemical bactericide occurs the most therewith.And chemical agent
The compounding consumption that can reduce a kind of microbiocidal composition, delays drug-fast generation, reduces production cost simultaneously, be plant disease
One of important measures in preventing and treating.
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant at present
Report and patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, balck shank
Antibacterial, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, the two
Weight ratio be 80:1～1:80, it is adaptable to the plant disease that caused by pathogenic fungi of preventing and treating, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of thiophene bacterium Han benzene
The bactericidal pesticide composition of amine, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, said composition
Can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide one to kill containing phenylamide, dinitroaniline, dithiocarbamate
The bacterium drug composition of microbial inoculum and biocontrol microorganisms, said composition has the preventing and treating of excellence to tobacco root-knot, black shank etc.
Effect, preventive effect is significantly better than and is used alone chemical bactericide or biocontrol microorganisms microbial inoculum, and any of the above-described or two series bactericidal agents and life
The combination of anti-bacteria agent, and chemical drug-fast generation can be delayed.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is killed by phenyl amide series bactericidal agent, dinitroaniline series bactericidal agent, dithiocarbamate
Microbial inoculum and biocontrol microorganisms mycopowder composition, by quality ratio, phenyl amide series bactericidal agent: dinitroaniline series bactericidal agent: dithio ammonia
Carbamate series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～10:1～10, in mycopowder, viable count is not less than
106cfu/g。
Described phenyl amide series bactericidal agent (PAFs) includes that acylalaninies series bactericidal agent is (such as metalaxyl, Metalaxyl-M, benzene
Frost M 9834 clever, smart, furalaxyl), butyrolactone series bactericidal agent (such as fenfuram) and oxazolidone series bactericidal agent (as white in disliked
Spirit) (Wang Hancheng, Li Wenhong, Feng Yonggang, etc. the history of black shank chemical prevention and present situation [J]. Chinese tobacco journal,
2011,17(5):96-102).Specifically be selected from metalaxyl, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram,
One or more in Wakil etc..As individually selected metalaxyl, M 9834 or furalaxyl, or select Metalaxyl-M and refining benzene
Frost spirit, metalaxyl and fenfuram, the combination of furalaxyl and Wakil, also or select M 9834, furalaxyl and Wakil
Combination.Compare the single a kind of phenyl amide series bactericidal agent of use, bactericide compounded (two kinds and above antibacterial with the use of) tool
There are summation action or potentiation.Further, in composite bactericide, each component proportion can arbitrarily be chosen, such as M 9834 and Wakil
Compounding according to mass ratio 1～10:1～10, M 9834 and furalaxyl, fenfuram are according to mass ratio 1～10:1～10:1～10
Compounding, etc..Owing to combining form is various, do not enumerate.
Described dinitroaniline series bactericidal agent is selected from fluazinam etc..
Described dithiocarbamate antibacterial selected from zineb, ambam, maneb, Carbatene, Mancozeb,
One or more in propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc..As individually selected zineb, Dai Sen
MnZn, Propineb or thiram, or select the combination of maneb and Propineb, propineb and thiram, also or choosing
With zineb, Propineb and the combination of ziram.Compare the single a kind of dithiocarbamate antibacterial of use, antibacterial
Compounding (two kinds and above antibacterial with the use of) have summation action or potentiation.Further, composite bactericide and biocontrol microorganisms
Prevention effect to tobacco root-knot, black shank etc. is used in combination be better than being used alone chemical bactericide or biocontrol microorganisms
Microbial inoculum.Meanwhile, in composite bactericide, each component proportion can arbitrarily be chosen, as Mancozeb and Propineb according to mass ratio 1～
10:1～10 compounds, and and for example zineb compounds according to mass ratio 1～10:1～10:1～10 with propineb, ziram, etc.
Deng.Owing to combining form is various, do not enumerate.
Preferably, in bacterium drug composition, phenyl amide series bactericidal agent is selected from metalaxyl, Metalaxyl-M, M 9834, refining benzene frost
Any one in spirit, furalaxyl, fenfuram, Wakil etc., dinitroaniline series bactericidal agent is fluazinam, dithiocarbamates
Formate ester antibacterial is selected from zineb, ambam, maneb, Carbatene, Mancozeb, propineb, Propineb, Fu Mei
Any one in double, ferric dimethyldithiocarbamate, ziram etc..The work of metalaxyl, furalaxyl, fenfuram etc. in phenyl amide series bactericidal agent
Same or similar by mechanism, in like manner dinitroaniline series bactericidal agent and dithiocarbamate antibacterial, therefore from above-mentioned
Choosing any one kind of them respectively in three series bactericidal agents compounding, composite bactericide is used in combination with biocontrol microorganisms again, can not only simplify formula, simultaneously
Tobacco root-knot, black shank etc. also can be reached good prevention effect.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Biocontrol microorganisms is preferably and solves
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13, deposit number: CGMCC No.6162, during preservation
Between: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, unit ground
Location: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can suppress cigarette
Grass black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of hold concurrently anti-root-knot nematode and
The Nicotiana tabacum L. endophyte of Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, phenylamide sterilizes
Agent, dinitroaniline series bactericidal agent, dithiocarbamate antibacterial are to bacillus amyloliquefaciens (CGMCC No.6162)
Growth effect faint, the compatibility between antibacterial and bacterial strain is good, can carry out bacterium medicine combination.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
Bacterium drug composition in the present invention is by phenyl amide series bactericidal agent, dinitroaniline series bactericidal agent, dithiocarbamates
Formate ester antibacterial and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder composition, wherein phenyl amide series bactericidal agent energy
The activity of RNA polymerase in suppression pathogen, the fluazinam of dinitroaniline can make the phosphorous oxide in pathogen Repiration
Acidifying uncoupling, hinders energy synthesis, the suppression mycelial growth of pathogen, Sporulation and sprouting, dithiocarbamate
Series bactericidal agent is multiaction site inhibitor, and is not likely to produce resistance, and this three series bactericidal agent coordinates with bacillus amyloliquefaciens to be made
Having excellent prevention effect with to tobacco root-knot, black shank, preventive effect is significantly better than and is used alone chemical sterilization
Agent or biocontrol microorganisms microbial inoculum, and the combination of any of the above-described or two series bactericidal agents and biocontrol microorganisms microbial inoculum, and chemistry Drug resistance can be delayed
Generation.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, by metalaxyl, fluazinam, ziram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder
Composition, by quality ratio, metalaxyl: fluazinam: ziram: bacillus amyloliquefaciens mycopowder=1:1:1:1, viable count in mycopowder
It is not less than 106cfu/g。
Embodiment 2
Bacterium drug composition, by Metalaxyl-M, fluazinam, Propineb and bacillus amyloliquefaciens (CGMCC No.6162) bacterium
Powder forms, and by quality ratio, Metalaxyl-M: fluazinam: Propineb: bacillus amyloliquefaciens mycopowder=3:5:8:10, in mycopowder
Viable count is not less than 106cfu/g。
Embodiment 3
Bacterium drug composition, by M 9834, fluazinam, maneb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder
Composition, by quality ratio, M 9834: fluazinam: maneb: bacillus amyloliquefaciens mycopowder=5:2:6:7, viable count in mycopowder
It is not less than 106cfu/g。
Embodiment 4
Bacterium drug composition, by essence M 9834, fluazinam, zineb and bacillus amyloliquefaciens (CGMCC No.6162) bacterium
Powder forms, by quality ratio, essence M 9834: fluazinam: zineb: bacillus amyloliquefaciens mycopowder=9:7:4:1, mycopowder is lived
Bacterium number is not less than 106cfu/g。
Embodiment 5
Bacterium drug composition, by furalaxyl, fluazinam, propineb and bacillus amyloliquefaciens (CGMCC No.6162)
Mycopowder forms, by quality ratio, and furalaxyl: fluazinam: propineb: bacillus amyloliquefaciens mycopowder=10:10:10:1,
In mycopowder, viable count is not less than 106cfu/g。
Embodiment 6
Bacterium drug composition, by fenfuram, fluazinam, thiram and bacillus amyloliquefaciens (CGMCC No.6162) bacterium
Powder forms, by quality ratio, fenfuram: fluazinam: thiram: bacillus amyloliquefaciens mycopowder=2:5:3:7, mycopowder is lived
Bacterium number is not less than 106cfu/g。
Embodiment 7
Bacterium drug composition, by Wakil, fluazinam, Mancozeb and bacillus amyloliquefaciens (CGMCC No.6162) bacterium
Powder forms, and by quality ratio, Wakil: fluazinam: Mancozeb: bacillus amyloliquefaciens mycopowder=1:1:1:10, in mycopowder
Viable count is not less than 106cfu/g。
Embodiment 8
Bacterium drug composition, by metalaxyl, fenfuram, fluazinam, Mancozeb, thiram and bacillus amyloliquefaciens
(CGMCC No.6162) mycopowder forms, by quality ratio, and metalaxyl: fenfuram: fluazinam: Mancozeb: thiram: solve
Bacillus amyloliquefaciens mycopowder=4:3:8:2:5:8, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 10 parts, fluazinam 25 parts, ziram 25 parts, solves
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 5 parts6Cfu/g), dodecyl sodium sulfate
6 parts, 5 parts of Laurel alcohol radical sodium sulfate, calcium lignosulfonate 4 parts, ethyl cellulose 5 parts, sucrose 4 parts, Kaolin 22 parts；Preparation side
Method is: accurately takes each raw material, grinding and sieving, mix homogeneously according to mass fraction, obtains wettable powder.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fenfuram 5 parts, fluazinam 5 parts, Propineb 10 parts, solves
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 50 parts6Cfu/g), calcium lignosulfonate 5
Part, sodium lauryl sulphate 4 parts, sucrose 5 parts, white carbon 15 parts, 16 parts of kieselguhr；Preparation method is with embodiment 9.
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 15 parts, fluazinam 22 parts, maneb 10 parts, solves
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 20 parts6Cfu/g), dodecyl sodium sulfonate
5 parts of sodium, 5 parts of Laurel alcohol radical sodium sulfate, sodium lauryl sulphate 5 parts, ethyl cellulose 3 parts, sucrose 5 parts, 15 parts of kieselguhr, recessed
15 parts of convex rod soil；Preparation method is with embodiment 9.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: M 9834 20 parts, fluazinam 15 parts, zineb 20 parts, solves
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 30 parts6Cfu/g), dodecyl naphthalene sulphur
5 parts of sodium of acid, pulls open 6 parts of powder, 3 parts of sodium sulfate, xanthan gum 10 parts, sucrose 5 parts, Kaolin 25 parts；Preparation method is: according to quality
Number accurately takes each raw material, pelletize after mix homogeneously, is dried, sieves, obtains suspending agent.
Embodiment 13
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Metalaxyl-M 15 parts, fluazinam 15 parts, Mancozeb 15
Part, (in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 5 parts6Cfu/g), dodecyl
Sodium naphthalene sulfonate 5 parts, pulls open 8 parts of powder, 3 parts of sodium sulfate, sucrose 3 parts, bentonite 10 parts, 18 parts of kieselguhr；Preparation method is with implementing
Example 12.
Embodiment 14
Bacterium medicine preparation, is made up of the raw material of following mass fraction: furalaxyl 15 parts, fluazinam 15 parts, propineb 15
Part, (in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 15 parts6Cfu/g), dodecyl
Sodium naphthalene sulfonate 5 parts, pulls open 4 parts of powder, starch 5 parts, 3 parts of sulphuric acid, xanthan gum 5 parts, sucrose 5 parts, bentonite 12 parts, kieselguhr 20
Part；Preparation method is with embodiment 12.
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: essence M 9834 8 parts, fluazinam 10 parts, thiram 18 parts,
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 35 parts6Cfu/g), lignin sulfonic acid
7 parts of sodium, nekal 3 parts, dodecylbenzene sodium sulfonate 4 parts, polyvinylpyrrolidone 3 parts, starch 4 parts, sucrose 3
Part, 22 parts of kieselguhr；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously according to mass fraction, makes
Grain, obtains water dispersion granule.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 10 parts, Wakil 12 parts, fluazinam 22 parts, generation
Gloomy MnZn 13 parts, thiram 15 parts, (in mycopowder, viable count is the lowest for bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 20 parts
In 106Cfu/g), polyvinylpyrrolidone 10 parts, dodecyl phenol polyethenoxy ether 5 parts, dodecylbenzene sodium sulfonate 6 parts, ring
Oxidative ethane 5 parts, ethyl cellulose 12 parts, attapulgite 28 parts；Preparation method is with embodiment 15.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can be respectively from phenyl amide series bactericidal agent, dithiocarbamate
The most multiple in antibacterial compound with fluazinam, composite bactericide again with biocontrol microorganisms combination or make bacterium medicine preparation.
Comparative example 1
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 17 parts, fluazinam 43 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with implementing
Example 9.
Comparative example 2
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 17 parts, ziram 43 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with implementing
Example 9.
Comparative example 3
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 30 parts, ziram 30 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with implementing
Example 9.
Comparative example 4
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 60 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and the same embodiment of preparation method
9。
Comparative example 5
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 60 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and the same embodiment of preparation method
9。
Comparative example 6
Bacterium medicine preparation, is made up of the raw material of following mass fraction: ziram 60 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and the same embodiment of preparation method
9。
Bacillus amyloliquefaciens (CGMCC No.6162) mycopowder in the various embodiments described above and comparative example is (public with reference to patent
Announcement CN102925393B) method is prepared as wettable powder in detailed description of the invention.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In table 1, bactericide original drug (not adding bacillus amyloliquefaciens) is 1.00 μ g/mL for examination concentration, kills in table 2
The former medicine of microbial inoculum (not adding bacillus amyloliquefaciens) is 0.30 μ g/mL for examination concentration, and in table 3, bactericide original drug (does not adds solution starch
Bacillus cereus) for examination concentration be 0.80 μ g/mL, in table 4 bactericide original drug (not adding bacillus amyloliquefaciens) for examination concentration
Being 3.50 μ g/mL, in table 5, phenylamide bactericide original drug (not adding bacillus amyloliquefaciens) is 0.05 μ g/ for examination concentration
ML, in table 6, dinitroaniline bactericide original drug (not adding bacillus amyloliquefaciens) is 1.00 μ g/mL for examination concentration, in table 7
Dithiocarbamate bactericide original drug (not adding bacillus amyloliquefaciens) is 10.00 μ g/mL for examination concentration；Solve starch
The concentration of bacillus cereus (CGMCC No.6162) bacteria suspension is 1010Cfu/mL, (all kinds of bactericide original drugs are corresponding for bacterium drug composition
Concentration+bacillus amyloliquefaciens (CGMCC No.6162) 1010Cfu/mL), compounding form is specifically shown in table 1 below～7.By diameter 5mm
Bacterium dish is placed in the oat medium central authorities of pastille, and every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation
Method measures colony diameter, calculates the different disposal suppression ratio to tobacco black shank bacterium, evaluates bacterium drug composition to black shank bacterium
Inhibitory action, result see table 1～7.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Table 1 chemical bactericide and the bacillus amyloliquefaciens inhibition to mycelial growth
Table 2 phenylamide, dinitroaniline series bactericidal agent and the bacillus amyloliquefaciens inhibition to mycelial growth
Mycelial growth is pressed down by table 3 phenylamide, dithiocarbamate antibacterial with bacillus amyloliquefaciens
Effect processed
Table 4 dinitroaniline, dithiocarbamate antibacterial and bacillus amyloliquefaciens are to mycelial growth
Inhibition
Table 5 phenyl amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 6 dinitroaniline series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Tobacco black shank bacterium mycelial growth is pressed down by table 7 dithiocarbamate antibacterial with bacillus amyloliquefaciens
Effect processed
From table 1～7 it can be seen that contain phenyl amide series bactericidal agent, dinitroaniline series bactericidal agent, aminodithioformic acid
The bacterium drug composition of esters antibacterial and bacillus amyloliquefaciens (CGMCC No.6162) inhibition to tobacco black shank bacterium
It is significantly better than and is used alone chemical bactericide or biocontrol microorganisms microbial inoculum, and any of the above-described or two series bactericidal agents and biocontrol microorganisms microbial inoculum
Combination.Show that the present invention has synergistic function between each component of bacterium drug composition.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculate tobacco black shank bacterium by greenhouse, and spray embodiment 9～16 and the bacterium medicine preparation of comparative example 1～6 preparation,
Concrete grammar is as follows: the method with reference to Dong Yu prepares bacterium paddy inoculum, and the bacterium cake of 20 diameter 5mm adds the paddy of 500g sterilizing
In son, 28 DEG C of cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. China's agriculture
Industry academy of science, 2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum,
When tobacco seedlings length to 6～7 slice true leaf, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, each process 5 strain tobacco seedlings,
Not apply medicament, to add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium in cigarette strain root after dispenser 3d
Paddy 10g/ strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 8.In table 8,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 68% essence first frost
MnZn water dispersible granules originates in industry strain purchased from Xian Zhengda crop protection company limited, 500g/L fluazinam suspending agent purchased from Japan's stone
Formula commercial firm, 50% thiram wettable powder is purchased from Shanxi Ke Li Science and Technology Ltd., bacillus amyloliquefaciens (CGMCC
No.6162) microbial inoculum is prepared with reference to method in patent (notification number CN102925393B) detailed description of the invention, is more than comparison
Group.
The table 8 bacterium medicine preparation prevention effect to black shank
As can be seen from Table 8, in embodiment 9～16, bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank,
Preventive effect reaches more than 90%, is better than bacterium medicine preparation in commercially available chemical agent, bacillus amyloliquefaciens microbial inoculum and comparative example 1～6, for
The preventing and treating of balck shank provides novel alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (10)
1. bacterium drug composition, it is characterised in that: by phenyl amide series bactericidal agent, dinitroaniline series bactericidal agent, dithiocarbamates
Formate ester antibacterial and biocontrol microorganisms mycopowder composition, by quality ratio, phenyl amide series bactericidal agent: dinitroaniline sterilizes
Agent: dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1～10:1～10:1～10:1～10, viable count in mycopowder
It is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described phenyl amide series bactericidal agent includes acyl group third
Propylhomoserin series bactericidal agent, butanoic acid lactone series bactericidal agent, oxazolidone series bactericidal agent.
Bacterium drug composition the most according to claim 2, it is characterised in that: described phenyl amide series bactericidal agent is selected from first frost
One or more in spirit, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram, Wakil.
Bacterium drug composition the most according to claim 1, it is characterised in that: described dinitroaniline series bactericidal agent is fluorine pyridine
Amine.
Bacterium drug composition the most according to claim 1, it is characterised in that: described dithiocarbamate antibacterial selects
From zineb, ambam, maneb, Carbatene, Mancozeb, propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram
In one or more.
6. according to the bacterium drug composition according to any one of Claims 1 to 5, it is characterised in that: described biocontrol microorganisms is for solving starch
Bacillus cereus ZY-9-13, deposit number: CGMCC No.6162.
7. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of claim 1～6.
Bacterium medicine preparation the most according to claim 7, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
9. as according to any one of claim 1～6 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
10. bacterium medicine preparation application in preventing and treating root knot nematode in tobacco and/or black shank as claimed in claim 7 or 8.
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