CN106106531A - Bacterium drug composition, preparation and application containing carboxylic acyloxy amine, Benzoylamide series bactericidal agent and biocontrol microorganisms - Google Patents
Bacterium drug composition, preparation and application containing carboxylic acyloxy amine, Benzoylamide series bactericidal agent and biocontrol microorganisms Download PDFInfo
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- CN106106531A CN106106531A CN201610493204.2A CN201610493204A CN106106531A CN 106106531 A CN106106531 A CN 106106531A CN 201610493204 A CN201610493204 A CN 201610493204A CN 106106531 A CN106106531 A CN 106106531A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
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Abstract
The invention discloses a kind of bacterium drug composition, preparation and application containing carboxylic acyloxy amine, Benzoylamide series bactericidal agent and biocontrol microorganisms, belong to plant protection and Pesticide Science field.This bacterium drug composition is made up of carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent and biocontrol microorganisms mycopowder, by quality ratio, carboxylic acid amide series bactericidal agent: Benzoylamide series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～10, in mycopowder, viable count is not less than 106cfu/g.Biocontrol microorganisms is preferably bacillus amyloliquefaciens ZY 9 13, deposit number: CGMCC No.6162.Wherein, carboxylic acid amide series bactericidal agent is inhibited to the synthesis of cause of disease oomycetes cell wall cellulose, the fluopicolide of benzamides reaches bactericidal effect by the spectrin acting on cell membrane, zoxamide is assembled by suppression pathogen mitochondrion β tubulin and plays bactericidal activity, and this two series bactericidal agent has excellent prevention effect with bacillus amyloliquefaciens with the use of to tobacco root-knot, black shank.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing carboxylic acyloxy amine, Benzoylamide series bactericidal agent and biocontrol microorganisms, simultaneously
Further relate to comprise preparation and the application of this bacterium drug composition, belong to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, and wherein the pesticide such as dimethomorph, fluopicolide is to produce
The medicament that middle use is relatively broad.Dimethomorph is the cinnamide sterilization researched and developed by American Cyanamid Co. the eighties in last century
Agent, has protection and the therapeutic effect of excellence to the oomycetes disease caused by Peronospora, Phytophthora etc., fine by suppression pathogen
Dimension element synthesis affects the normally forming of cell wall, and (Cui Xiaolan, Meng Qingxiao, Bi Yang, etc. the Phytophthora capsici sensitivity to dimethomorph
Baseline and indoor drug resistant mutants research [J]. Plant Pathology, 2009).Fluopicolide is the benzene first researched and developed by Beyer Co., Ltd
Acid amide fungicides, efficient to cause of disease oomycetes, reach bactericidal effect by acting on the spectrin of cell membrane, its uniqueness
Mechanism of action make its with various sterilization agent equal no interactions Drug resistance (roc. fluopicolide and pyraclostrobin are to Rhizoma Solani tuber osi late blight
The co-toxicity of pathogenic bacteria and mixture preparation research [D]. Agricultural University Of Hebei, 2013).But be as in producing is commonly used,
The resistance problem of chemical bactericide occurs the most therewith.And the compounding consumption that can reduce a kind of microbiocidal composition of chemical agent, prolong
Slow drug-fast generation, reduces production cost simultaneously, is one of important measures in control of plant disease.
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant at present
Report and patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, balck shank
Antibacterial, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, the two
Weight ratio be 80:1～1:80, it is adaptable to the plant disease that caused by pathogenic fungi of preventing and treating, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of thiophene bacterium Han benzene
The bactericidal pesticide composition of amine, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, said composition
Can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide the combination of a kind of bacterium medicine containing carboxylic acyloxy amine, Benzoylamide series bactericidal agent and biocontrol microorganisms
Thing, said composition has the prevention effect of excellence to tobacco root-knot, black shank etc., and it is single that preventive effect is significantly better than use
The antibacterial of one kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and can delaying
Learn drug-fast generation.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is made up of carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent and biocontrol microorganisms mycopowder, with quality
Than meter, carboxylic acid amide series bactericidal agent: Benzoylamide series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～10, mycopowder is lived
Bacterium number is not less than 106cfu/g。
Chemically in structure divide, described carboxylic acid amide series bactericidal agent include cinnamamide series bactericidal agent (as dimethomorph,
Flumorph, pyrimorph), mandelic acidamide series bactericidal agent (such as mandipropamid) and valine amide carbamate fungicide
(such as iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal) three chemical subgroups (inteilectual Zhu, Lu Xiaohong, Chen Lei,
Deng. carboxylic acyloxy amine (CAAs) antibacterial progress [J]. Pesticide Science journal, 2010,12 (1): 1-12).Specifically it is selected from alkene
In morpholide, flumorph, pyrimorph, mandipropamid, iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal etc.
Any one or more.As individually selected flumorph, benzene metsulfovax or mandipropamid, or select dimethomorph and double alkynes
Acyl bacterium amine, pyrimorph and iprovalicarb, the combination of different benzene metsulfovax and mandipropamid, also or select flumorph, benzene thiophene
Bacterium amine and the combination of mandipropamid.Compare the single a kind of carboxylic acid amide series bactericidal agent of use, bactericide compounded (two kinds and more than
Antibacterial with the use of) there is summation action or potentiation.Meanwhile, in composite bactericide, each component proportion can arbitrarily select
Take, as dimethomorph and mandipropamid compound according to mass ratio 1～10:1～10, and for example dimethomorph and benzene metsulfovax, double
Mandipropamid compounds according to mass ratio 1～10:1～10:1～10, etc..Owing to combining form is various, do not enumerate.
Described Benzoylamide series bactericidal agent any one or more in fluopicolide, zoxamide etc..As individually
Select fluopicolide or zoxamide, or select the combination of fluopicolide and zoxamide.Compare the single a kind of benzene first of use
Acid amide fungicides, bactericide compounded (two kinds and above antibacterial with the use of) have summation action or potentiation.Further,
In composite bactericide, each component proportion can arbitrarily be chosen, if fluopicolide and zoxamide are according to mass ratio 1～10:1～10
Combination, etc..Wherein, the sterilization mechanism of fluopicolide is entirely different with current all known Oomycete antibacterial, mainly makees
Showing bactericidal activity for cell membrane and intercellular specific proteins, the thin layer penetrance of its uniqueness can strengthen the horizontal stroke of medicament
To conductivity and longitudinally fed power, good inhibitory activity, treatment potential all there is is to highlight each Main Morphology of pathogen.Benzoyl
Bacterium amine is assembled by the mitochondrion β-tubulin of suppression pathogen and plays bactericidal activity.
Preferably, in bacterium drug composition, carboxylic acid amide series bactericidal agent is selected from dimethomorph, flumorph, pyrimorph, double alkynes
Any one in acyl bacterium amine, iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal etc., Benzoylamide series bactericidal agent
Any one in fluopicolide, zoxamide etc..Dimethomorph in carboxylic acid amide series bactericidal agent, mandipropamid, different
The mechanism of action of the third bacterium amine etc. is same or similar, in like manner Benzoylamide series bactericidal agent, therefore from above-mentioned two series bactericidal agents respectively
Choosing any one kind of them compounding, composite bactericide is used in combination with biocontrol microorganisms again, can not only simplify formula, simultaneously to root knot nematode in tobacco
Disease, black shank etc. also can reach good prevention effect.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Biocontrol microorganisms is preferably and solves
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13, deposit number: CGMCC No.6162, during preservation
Between: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, unit ground
Location: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can suppress cigarette
Grass black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of hold concurrently anti-root-knot nematode and
The Nicotiana tabacum L. endophyte of Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, carboxylic acyloxy amine sterilizes
Agent, Benzoylamide series bactericidal agent are faint to the growth effect of bacillus amyloliquefaciens (CGMCC No.6162), antibacterial and bacterial strain
Between the compatibility good, bacterium medicine combination can be carried out.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is by carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent and bacillus amyloliquefaciens
(CGMCC No.6162) mycopowder forms, and wherein the synthesis of cause of disease oomycetes cell wall cellulose is had and presses down by carboxylic acid amide series bactericidal agent
Making use, the fluopicolide of benzamides reaches bactericidal effect by the spectrin acting on cell membrane, and zoxamide leads to
Crossing suppression pathogen mitochondrion β-tubulin and assemble performance bactericidal activity, this two series bactericidal agent coordinates with bacillus amyloliquefaciens
Using and tobacco root-knot, black shank are had excellent prevention effect, preventive effect is significantly better than and uses single kind
Antibacterial, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and chemistry Drug resistance can be delayed
Generation, be particularly well-suited to the preventing and treating of field tobacco balck shank.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, by dimethomorph, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, dimethomorph: fluopicolide: bacillus amyloliquefaciens mycopowder=1:1:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of flumorph, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, flumorph: zoxamide: bacillus amyloliquefaciens mycopowder=5:2:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 3
Bacterium drug composition, by pyrimorph, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, pyrimorph: fluopicolide: bacillus amyloliquefaciens mycopowder=1:10:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 4
Bacterium drug composition, by mandipropamid, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, mandipropamid: fluopicolide: bacillus amyloliquefaciens mycopowder=2:5:10, in mycopowder, viable count is the lowest
In 106cfu/g。
Embodiment 5
Bacterium drug composition, by iprovalicarb, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, iprovalicarb: zoxamide: bacillus amyloliquefaciens mycopowder=10:10:1, in mycopowder, viable count is the lowest
In 106cfu/g。
Embodiment 6
Bacterium drug composition, by benzene metsulfovax, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, benzene metsulfovax: zoxamide: bacillus amyloliquefaciens mycopowder=1:1:10, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 7
Bacterium drug composition, by flumorph, different benzene metsulfovax, fluopicolide and bacillus amyloliquefaciens (CGMCC
No.6162) mycopowder composition, by quality ratio, flumorph: different benzene metsulfovax: fluopicolide: bacillus amyloliquefaciens mycopowder=4:
2:1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 8
Bacterium drug composition, by valiphenal, fluopicolide, zoxamide and bacillus amyloliquefaciens (CGMCC
No.6162) mycopowder composition, by quality ratio, valiphenal: fluopicolide: benzene metsulfovax: bacillus amyloliquefaciens mycopowder=
7:3:6:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: benzene metsulfovax 22 parts, fluopicolide 15 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 10 parts6Cfu/g), calcium lignosulfonate 6 parts, ten
Sodium dialkyl sulfate 8 parts, sucrose 4 parts, white carbon 10 parts, 17 parts of kieselguhr；Preparation method is: accurately take each according to mass fraction
Raw material, grinding and sieving, mix homogeneously, obtain wettable powder.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: valiphenal 5 parts, zoxamide 5 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 45 parts6Cfu/g), dodecyl sodium sulfate 5 parts,
6 parts of Laurel alcohol radical sodium sulfate, sodium lauryl sulphate 7 parts, ethyl cellulose 3 parts, sucrose 6 parts, 10 parts of kieselguhr, attapulgite
10 parts；Preparation method is with embodiment 9.
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: iprovalicarb 18 parts, fluopicolide 35 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 17 parts6Cfu/g), dodecyl sodium sulfate 12 parts,
8 parts of Laurel alcohol radical sodium sulfate, calcium lignosulfonate 6 parts, ethyl cellulose 10 parts, sucrose 3 parts, Kaolin 18 parts；Preparation method
With embodiment 9.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: mandipropamid 10 parts, fluopicolide 15 parts, solves starch
(in mycopowder, viable count is not less than 10 to bacillus cereus (CGMCC No.6162) mycopowder 32 parts6Cfu/g), dodecyl sodium naphthalene sulfonate
10 parts, pull open 3 parts of powder, starch 3 parts, 3 parts of sodium sulfate, xanthan gum 4 parts, sucrose 6 parts, bentonite 14 parts, 18 parts of kieselguhr；Preparation
Method is: accurately take each raw material, pelletize after mix homogeneously according to mass fraction, is dried, sieves, obtains suspending agent.
Embodiment 13
Bacterium medicine preparation, is made up of the raw material of following mass fraction: pyrimorph 18 parts, zoxamide 14 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 22 parts6cfu/g), dodecyl sodium naphthalene sulfonate 6 parts,
Pull open 12 parts of powder, 3 parts of sodium sulfate, xanthan gum 14 parts, sucrose 3 parts, Kaolin 17 parts；Preparation method is with embodiment 12.
Embodiment 14
Bacterium medicine preparation, is made up of the raw material of following mass fraction: flumorph 38 parts, zoxamide 16 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 15 parts6cfu/g), dodecyl sodium naphthalene sulfonate 5 parts, draws
Open 8 parts of powder, 3 parts of sodium sulfate, sucrose 5 parts, bentonite 16 parts, 15 parts of kieselguhr；Preparation method is with embodiment 12.
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: dimethomorph 16 parts, fluopicolide 8 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 25 parts6cfu/g), sodium lignin sulfonate 12 parts, two fourths
Base sodium naphthalene sulfonate 3 parts, dodecylbenzene sodium sulfonate 3 parts, polyvinylpyrrolidone 5 parts, starch 3 parts, sucrose 5 parts, kieselguhr 15
Part；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtains water-dispersible
Granule.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: flumorph 10 parts, different benzene metsulfovax 5 parts, fluopicolide 8
Part, zoxamide 6 parts, (in mycopowder, viable count is not less than bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 30 parts
106cfu/g), polyvinylpyrrolidone 7 parts, dodecyl phenol polyethenoxy ether 4 parts, dodecylbenzene sodium sulfonate 5 parts, epoxy second
3 parts of alkane, ethyl cellulose 12 parts, attapulgite 15 parts；Preparation method is with embodiment 15.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can from carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent optionally
Multiple compounding, composite bactericide combines with biocontrol microorganisms or makes bacterium medicine preparation.
Comparative example 1
Bacterium medicine preparation, is made up of the raw material of following mass fraction: iprovalicarb 53 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 17 parts6cfu/g), other auxiliary agents and the same embodiment of preparation method
11。
Comparative example 2
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 53 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 17 parts6cfu/g), other auxiliary agents and the same embodiment of preparation method
11。
Bacillus amyloliquefaciens (CGMCC No.6162) mycopowder in the various embodiments described above and comparative example is (public with reference to patent
Announcement CN102925393B) method is prepared as wettable powder in detailed description of the invention.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In table 1, bactericide original drug (not adding bacillus amyloliquefaciens) is 0.20 μ g/mL for examination concentration, carboxylic in table 2
The acid former medicine of acid amide fungicides (not adding bacillus amyloliquefaciens) is 0.25 for examination concentrationμG/mL, in table 3, benzamides kills
The former medicine of microbial inoculum (not adding bacillus amyloliquefaciens) is 0.15 for examination concentrationμg/mL；Bacillus amyloliquefaciens (CGMCC
No.6162) concentration of bacteria suspension is 1010Cfu/mL, bacterium drug composition (all kinds of bactericide original drug corresponding concentration+solution starch spore
Bacillus (CGMCC No.6162) 1010Cfu/mL), compounding form is specifically shown in table 1 below～3.Diameter 5mm bacterium dish is placed in pastille
Oat medium central authorities, every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation method to measure colony diameter,
Calculating the different disposal suppression ratio to tobacco black shank bacterium, evaluate the bacterium drug composition inhibitory action to black shank bacterium, result is shown in
Table 1 below～3.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculatingsynergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Table 1 carboxylic acyloxy amine, Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to mycelial growth
Table 2 carboxylic acid amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 3 Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
From table 1～3 it can be seen that contain carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent and bacillus amyloliquefaciens
The bacterium drug composition of (CGMCC No.6162) is significantly better than the sterilization using single kind to the inhibition of tobacco black shank bacterium
Agent, bacillus amyloliquefaciens and any of the above-described kind of series bactericidal agent and the combination of bacillus amyloliquefaciens.Show bacterium in the present invention
Between each component of drug composition, there is synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculate tobacco black shank bacterium by greenhouse, and spray embodiment 9～16 and the bacterium medicine preparation of comparative example 1～2 preparation,
Concrete grammar is as follows: the method with reference to Dong Yu prepares bacterium paddy inoculum, and the bacterium cake of 20 diameter 5mm adds the paddy of 500g sterilizing
In son, 28 DEG C of cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. China's agriculture
Industry academy of science, 2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum,
When tobacco seedlings length to 6～7 slice true leaf, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, each process 5 strain tobacco seedlings,
Not apply medicament, to add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium in cigarette strain root after dispenser 3d
Paddy 10g/ strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 4.In table 4,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 50% dimethomorph
Wettable powder is purchased from BASF AG, and 687.5g/L Yin Fali (fluorine bacterium Propamocarb) is purchased from Beyer Co., Ltd, solves starch spore
Bacillus (CGMCC No.6162) microbial inoculum is prepared with reference to method in patent (notification number CN102925393B) detailed description of the invention, with
On be matched group.
The table 4 bacterium medicine preparation prevention effect to black shank
From table 4, it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 9～16,
Preventive effect reaches more than 83%, is better than bacterium medicine preparation in commercially available chemical agent, bacillus amyloliquefaciens microbial inoculum and comparative example 1～2, for
The preventing and treating of balck shank provides novel alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (9)
1. bacterium drug composition, it is characterised in that: by carboxylic acid amide series bactericidal agent, Benzoylamide series bactericidal agent and biocontrol microorganisms mycopowder group
Become, by quality ratio, carboxylic acid amide series bactericidal agent: Benzoylamide series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～
10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described carboxylic acid amide series bactericidal agent includes cinnamoyl
Amine antibacterial, mandelic acidamide series bactericidal agent, valine amide carbamate fungicide.
Bacterium drug composition the most according to claim 2, it is characterised in that: described carboxylic acid amide series bactericidal agent is selected from alkene acyl
Any in quinoline, flumorph, pyrimorph, mandipropamid, iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal
One or more.
Bacterium drug composition the most according to claim 1, it is characterised in that: described Benzoylamide series bactericidal agent is selected from fluorine pyrrole bacterium
Any one or two kinds in amine, zoxamide.
5. according to the bacterium drug composition according to any one of Claims 1 to 4, it is characterised in that: described biocontrol microorganisms is for solving starch
Bacillus cereus ZY-9-13, deposit number: CGMCC No.6162.
6. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of Claims 1 to 5.
Bacterium medicine preparation the most according to claim 6, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
8. as according to any one of Claims 1 to 5 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
The application in preventing and treating root knot nematode in tobacco and/or black shank of the bacterium medicine preparation the most as claimed in claims 6 or 7.
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