CN106035379A - Bactericide composition containing phenylamide and benzamide bactericides and biocontrol bacterium, preparation and applications - Google Patents
Bactericide composition containing phenylamide and benzamide bactericides and biocontrol bacterium, preparation and applications Download PDFInfo
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
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Abstract
The invention discloses a bactericide composition containing phenylamide and benzamide bactericides and a biocontrol bacterium, a preparation and applications and belongs to the field of plant protection and pesticide science. The bactericide composition comprises phenylamide bactericides, benzamide bactericides and biocontrol bacterium powder, wherein the mass ratio of the phenylamide bactericides to the benzamide bactericides to the biocontrol bacterium powder is (1-10) to (1-10) to (1-10); the viable count of the bacterium powder is not lower than 10<6>cfu/g; the biocontrol bacterium is preferably bacillus amyloliquefaciens ZY-9-13, with collection number of CGMCC No.6162. The bactericide composition has the beneficial effects that the phenylamide bactericides can inhibit the activity of RNA polymerase in pathogenic bacteria; fluopicolide as the benzamide bactericide achieves the bactericidal effects via spectrin acting on cell membranes; zoxamide gives play to the bactericidal activity by inhibiting assembly of a mitochondrion beta-tubulin of pathogenic bacteria; by using the two types of bactericides with bacillus amyloliquefaciens cooperatively, the bactericide composition has excellent control effects on tobacco root knot nematode and tobacco black shank.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing phenylamide, Benzoylamide series bactericidal agent and biocontrol microorganisms, simultaneously
Further relate to comprise preparation and the application of this bacterium drug composition, belong to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, during wherein the pesticide such as metalaxyl, fluopicolide is production
Use relatively broad medicament.Metalaxyl is phenylamide systemic fungicide, the mid-80 at China's production and application,
Playing an important role in oomycetes disease control, its mechanism of action is the polymerase activity of suppression pathogen, and RNA interfering synthesizes.
Fluopicolide is the Benzoylamide series bactericidal agent researched and developed by Beyer Co., Ltd, efficient to cause of disease oomycetes, by acting on cell membrane
Spectrin and reach bactericidal effect, the mechanism of action of its uniqueness make its with various sterilization agent equal no interactions Drug resistance (roc. fluorine
Pyrrole bacterium amine and the co-toxicity of pyraclostrobin Phytophthora infestans and mixture preparation study [D]. Agricultural University Of Hebei,
2013).But be as in producing is commonly used, and the resistance problem of chemical bactericide occurs the most therewith.And chemical agent compounds
The consumption of a kind of microbiocidal composition can be reduced, delay drug-fast generation, reduce production cost simultaneously, be control of plant disease
In one of important measures.
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant at present
Report and patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, balck shank
Antibacterial, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, the two
Weight ratio be 80:1～1:80, it is adaptable to the plant disease that caused by pathogenic fungi of preventing and treating, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of thiophene bacterium Han benzene
The bactericidal pesticide composition of amine, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, said composition
Can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide the combination of a kind of bacterium medicine containing phenylamide, Benzoylamide series bactericidal agent and biocontrol microorganisms
Thing, said composition has the prevention effect of excellence to tobacco root-knot, black shank etc., and it is single that preventive effect is significantly better than use
The antibacterial of one kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and can delaying
Learn drug-fast generation.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is made up of phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent and biocontrol microorganisms mycopowder, with quality
Than meter, phenyl amide series bactericidal agent: Benzoylamide series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～10, mycopowder is lived
Bacterium number is not less than 106cfu/g。
Described phenyl amide series bactericidal agent (PAFs) includes that acylalaninies series bactericidal agent is (such as metalaxyl, Metalaxyl-M, benzene
Frost M 9834 clever, smart, furalaxyl), butyrolactone series bactericidal agent (such as fenfuram) and oxazolidone series bactericidal agent (as white in disliked
Spirit) (Wang Hancheng, Li Wenhong, Feng Yonggang, etc. the history of black shank chemical prevention and present situation [J]. Chinese tobacco journal,
2011,17(5):96-102).Specifically be selected from metalaxyl, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram,
One or more in Wakil etc..As individually selected metalaxyl, M 9834 or furalaxyl, or select Metalaxyl-M and refining benzene
Frost spirit, metalaxyl and fenfuram, the combination of furalaxyl and Wakil, also or select M 9834, furalaxyl and Wakil
Combination.Compare the single a kind of phenyl amide series bactericidal agent of use, bactericide compounded (two kinds and above antibacterial with the use of) tool
There are summation action or potentiation.Further, in composite bactericide, each component proportion can arbitrarily be chosen, such as M 9834 and Wakil
Compounding according to mass ratio 1～10:1～10, M 9834 and furalaxyl, fenfuram are according to mass ratio 1～10:1～10:1～10
Compounding, etc..Owing to combining form is various, do not enumerate.
Described Benzoylamide series bactericidal agent any one or more in fluopicolide, zoxamide etc..As individually
Select fluopicolide or zoxamide, or select the combination of fluopicolide and zoxamide.Compare the single a kind of benzene first of use
Acid amide fungicides, bactericide compounded (two kinds and above antibacterial with the use of) have summation action or potentiation.Further,
In composite bactericide, each component proportion can arbitrarily be chosen, if fluopicolide and zoxamide are according to mass ratio 1～10:1～10
Combination, etc..
Preferably, in bacterium drug composition, phenyl amide series bactericidal agent is selected from metalaxyl, Metalaxyl-M, M 9834, refining benzene frost
Any one in spirit, furalaxyl, fenfuram, Wakil etc., Benzoylamide series bactericidal agent is selected from fluopicolide, zoxamide
Any one in Deng.In phenyl amide series bactericidal agent, the mechanism of action of metalaxyl, furalaxyl, fenfuram etc. is identical or phase
Seemingly, in like manner Benzoylamide series bactericidal agent, therefore choose any one kind of them respectively from above-mentioned two series bactericidal agents compounding, composite bactericide again with
Biocontrol microorganisms is used in combination, and can not only simplify formula, also can reach tobacco root-knot, black shank etc. good simultaneously
Prevention effect.The metalaxyl of phenylamide, M 9834, Wakil etc. are the interior suction sterilizations that a class has protection and therapeutical effect
Agent, can be absorbed rapidly by plant root, leaf, the tip, and by the non-living space systems such as fascicular conduit and intercellular substance to
Plant top is shifted, and has the highest activity to phytophthora, can be used for stem, leaf, seed and soil treatment.Kill at benzamides
In microbial inoculum, the sterilization mechanism of fluopicolide is entirely different with current all known Oomycete antibacterial, mainly acts on cell
Film and intercellular specific proteins and show bactericidal activity, the thin layer penetrance of its uniqueness can strengthen the lateral conductivity of medicament
And longitudinally fed power, all there are good inhibitory activity, treatment potential to highlight each Main Morphology of pathogen.Zoxamide passes through
Mitochondrion β-the tubulin of suppression pathogen assembles and plays bactericidal activity.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Biocontrol microorganisms is preferably and solves
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13, deposit number: CGMCC No.6162, during preservation
Between: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, unit ground
Location: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can suppress cigarette
Grass black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of hold concurrently anti-root-knot nematode and
The Nicotiana tabacum L. endophyte of Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, phenylamide sterilizes
Agent, Benzoylamide series bactericidal agent are faint to the growth effect of bacillus amyloliquefaciens (CGMCC No.6162), antibacterial and bacterial strain
Between the compatibility good, bacterium medicine combination can be carried out.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is by phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent and bacillus amyloliquefaciens
(CGMCC No.6162) mycopowder forms, and wherein phenyl amide series bactericidal agent can suppress the activity of RNA polymerase, benzene in pathogen
The fluopicolide of benzamide type reaches bactericidal effect by the spectrin acting on cell membrane, and zoxamide is by suppression cause of disease
Bacterium mitochondrion β-tubulin assembles and plays bactericidal activity, and this two series bactericidal agent and bacillus amyloliquefaciens are with the use of to Nicotiana tabacum L.
Root knot nematode disease, black shank have the prevention effect of excellence, and preventive effect is significantly better than the use antibacterial of single kind, biological and ecological methods to prevent plant disease, pests, and erosion
The combination of bacteria agent and any of the above-described kind of series bactericidal agent and biocontrol microorganisms microbial inoculum, and chemical drug-fast generation can be delayed, especially
It is applicable to the preventing and treating of field tobacco balck shank.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of metalaxyl, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, metalaxyl: fluopicolide: bacillus amyloliquefaciens mycopowder=1:1:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of M 9834, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, M 9834: zoxamide: bacillus amyloliquefaciens mycopowder=6:3:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 3
Bacterium drug composition, by Metalaxyl-M, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, Metalaxyl-M: fluopicolide: bacillus amyloliquefaciens mycopowder=1:10:3, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 4
Bacterium drug composition, by fenfuram, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, fenfuram: fluopicolide: bacillus amyloliquefaciens mycopowder=5:5:1, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 5
Bacterium drug composition, is made up of Wakil, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, Wakil: zoxamide: bacillus amyloliquefaciens mycopowder=1:2:10, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 6
Bacterium drug composition, is made up of furalaxyl, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, furalaxyl: zoxamide: bacillus amyloliquefaciens mycopowder=1:1:10, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 7
Bacterium drug composition, by essence M 9834, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, essence M 9834: fluopicolide: bacillus amyloliquefaciens mycopowder=10:10:1, in mycopowder, viable count is the lowest
In 106cfu/g。
Embodiment 8
Bacterium drug composition, by metalaxyl, fenfuram, fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162)
Mycopowder forms, by quality ratio, and metalaxyl: fenfuram: fluopicolide: bacillus amyloliquefaciens mycopowder=5:5:6:7, mycopowder
Middle viable count is not less than 106cfu/g。
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 25 parts, fluopicolide 25 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 5 parts6Cfu/g), dodecyl sodium sulfate 8 parts, Laurel
3 parts of alcohol radical sodium sulfate, sodium lauryl sulphate 6 parts, ethyl cellulose 3 parts, sucrose 10 parts, 12 parts of kieselguhr, attapulgite 10
Part；Preparation method is: accurately takes each raw material, grinding and sieving, mix homogeneously according to mass fraction, obtains wettable powder.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: essence M 9834 5 parts, zoxamide 5 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 45 parts6Cfu/g), calcium lignosulfonate 10 parts, 12
Alkyl sodium sulfate 3 parts, sucrose 4 parts, white carbon 15 parts, 12 parts of kieselguhr；Preparation method is with embodiment 9.
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: furalaxyl 15 parts, fluopicolide 18 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 35 parts6Cfu/g), dodecyl sodium sulfate 12 parts, the moon
10 parts of cinnamic alcohol base sodium sulfate, calcium lignosulfonate 6 parts, ethyl cellulose 10 parts, sucrose 5 parts, Kaolin 18 parts；Preparation method is same
Embodiment 9.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 15 parts, zoxamide 16 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 22 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 6 parts,
Pull open 8 parts of powder, 2 parts of sodium sulfate, xanthan gum 10 parts, sucrose 4 parts, Kaolin 16 parts；Preparation method is: accurate according to mass fraction
Take each raw material, pelletize after mix homogeneously, it is dried, sieves, obtain suspending agent.
Embodiment 13
Bacterium medicine preparation, is made up of the raw material of following mass fraction: M 9834 15 parts, fluopicolide 20 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 22 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 10 parts,
Pull open 3 parts of powder, starch 3 parts, 2 parts of sodium sulfate, xanthan gum 5 parts, sucrose 6 parts, bentonite 15 parts, 15 parts of kieselguhr；Preparation method
With embodiment 12.
Embodiment 14
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Metalaxyl-M 15 parts, zoxamide 20 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 35 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 6
Part, pull open 10 parts of powder, 3 parts of sodium sulfate, sucrose 5 parts, bentonite 10 parts, 17 parts of kieselguhr；Preparation method is with embodiment 12.
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fenfuram 20 parts, fluopicolide 22 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 20 parts6Cfu/g), polyvinylpyrrolidone 7 parts,
Dodecyl phenol polyethenoxy ether 3 parts, dodecylbenzene sodium sulfonate 6 parts, 5 parts of oxirane, ethyl cellulose 12 parts, attapulgite
12 parts of soil；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtains water
Dispersible granule.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: M 9834 15 parts, Wakil 10 parts, fluopicolide 20 parts,
(in mycopowder, viable count is not less than 10 to bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 15 parts6Cfu/g), lignin sulfonic acid
8 parts of sodium, nekal 3 parts, dodecylbenzene sodium sulfonate 4 parts, polyvinylpyrrolidone 5 parts, starch 3 parts, sucrose 5
Part, 20 parts of kieselguhr；Preparation method is with embodiment 15.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can from phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent optionally
Multiple compounding, composite bactericide combines with biocontrol microorganisms or makes bacterium medicine preparation.
Comparative example 1
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 50 parts, bacillus amyloliquefaciens
(CGMCCNo.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and the same embodiment of preparation method
9。
Comparative example 2
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 50 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with embodiment 9.
Bacillus amyloliquefaciens (CGMCC No.6162) mycopowder in the various embodiments described above and comparative example is (public with reference to patent
Announcement CN102925393B) method is prepared as wettable powder in detailed description of the invention.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In table 1, bactericide original drug (not adding bacillus amyloliquefaciens) is 0.08 μ g/mL for examination concentration, benzene in table 2
The former medicine of base acid amide fungicides (not adding bacillus amyloliquefaciens) is 0.05 μ g/mL for examination concentration, and in table 3, benzamides kills
The former medicine of microbial inoculum (not adding bacillus amyloliquefaciens) is 0.15 μ g/mL for examination concentration；Bacillus amyloliquefaciens (CGMCC
No.6162) concentration of bacteria suspension is 1010Cfu/mL, bacterium drug composition (all kinds of bactericide original drug corresponding concentration+solution starch spore
Bacillus (CGMCC No.6162) 1010Cfu/mL), compounding form is specifically shown in table 1 below～3.Diameter 5mm bacterium dish is placed in pastille
Oat medium central authorities, every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation method to measure colony diameter,
Calculating the different disposal suppression ratio to tobacco black shank bacterium, evaluate the bacterium drug composition inhibitory action to black shank bacterium, result is shown in
Table 1 below～3.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Table 1 phenylamide, Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to mycelial growth
Table 2 phenyl amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 3 Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
From table 1～3 it can be seen that contain phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent and bacillus amyloliquefaciens
The bacterium drug composition of (CGMCC No.6162) is significantly better than the sterilization using single kind to the inhibition of tobacco black shank bacterium
Agent, bacillus amyloliquefaciens and any of the above-described kind of series bactericidal agent and the combination of bacillus amyloliquefaciens.Show bacterium in the present invention
Between each component of drug composition, there is synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculate tobacco black shank bacterium by greenhouse, and spray embodiment 9～16 and the bacterium medicine preparation of comparative example 1～2 preparation,
Concrete grammar is as follows: the method with reference to Dong Yu prepares bacterium paddy inoculum, and the bacterium cake of 20 diameter 5mm adds the paddy of 500g sterilizing
In son, 28 DEG C of cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. China's agriculture
Industry academy of science, 2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum,
When tobacco seedlings length to 6～7 slice true leaf, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, each process 5 strain tobacco seedlings,
Not apply medicament, to add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium in cigarette strain root after dispenser 3d
Paddy 10g/ strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 4.In table 4,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 68% essence first frost
MnZn water dispersible granules is purchased from Bayer purchased from Xian Zhengda crop protection company limited, 687.5g/L Yin Fali (fluorine bacterium Propamocarb)
Company, bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum is with reference to patent (notification number CN102925393B) specific embodiment party
In formula prepared by method, is more than matched group.
The table 4 bacterium medicine preparation prevention effect to black shank
From table 4, it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 9～16,
Preventive effect reaches more than 82%, is better than bacterium medicine preparation in commercially available chemical agent, bacillus amyloliquefaciens microbial inoculum and comparative example 1～2, for
The preventing and treating of balck shank provides novel alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (9)
1. bacterium drug composition, it is characterised in that: by phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent and biocontrol microorganisms mycopowder group
Become, by quality ratio, phenyl amide series bactericidal agent: Benzoylamide series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10:1～
10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described phenyl amide series bactericidal agent includes acyl group third
Propylhomoserin series bactericidal agent, butanoic acid lactone series bactericidal agent, oxazolidone series bactericidal agent.
Bacterium drug composition the most according to claim 2, it is characterised in that: described phenyl amide series bactericidal agent is selected from first frost
Any one or more in spirit, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram, Wakil.
Bacterium drug composition the most according to claim 1, it is characterised in that: described Benzoylamide series bactericidal agent is selected from fluorine pyrrole bacterium
Any one or two kinds in amine, zoxamide.
5. according to the bacterium drug composition according to any one of Claims 1 to 4, it is characterised in that: described biocontrol microorganisms is for solving starch
Bacillus cereus ZY-9-13, deposit number: CGMCC No.6162.
6. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of Claims 1 to 5.
Bacterium medicine preparation the most according to claim 6, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
8. as according to any one of Claims 1 to 5 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
The application in preventing and treating root knot nematode in tobacco and/or black shank of the bacterium medicine preparation the most as claimed in claims 6 or 7.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228255A (en) * | 1998-01-27 | 1999-09-15 | 罗姆和哈斯公司 | Fungicidal compositions containing N-acetonylbenzamides |
| CN101647435A (en) * | 2009-07-21 | 2010-02-17 | 深圳诺普信农化股份有限公司 | Synthetical sterilization composition |
| CN101999356A (en) * | 2010-11-26 | 2011-04-06 | 惠州市中迅化工有限公司 | Sterilization composition containing fluopicolide and metalaxyl and application thereof |
| CN102246760A (en) * | 2011-04-30 | 2011-11-23 | 江西海科瑞特作物科学有限公司 | Bactericidal composition containing fluopicolide and metalaxyl |
| CN102480965A (en) * | 2009-06-24 | 2012-05-30 | 拜耳作物科学公司 | Combinations of fungicidally active yeast and fungicides |
| CN102925393A (en) * | 2012-11-07 | 2013-02-13 | 中国烟草总公司郑州烟草研究院 | Tobacco endophyte resisting meloidogyne and tobacco phytophthora nicotianae and application thereof |
| CN103004819A (en) * | 2012-11-30 | 2013-04-03 | 王学权 | Fluopicolide-containing bactericide composition |
| CN103651353A (en) * | 2013-12-18 | 2014-03-26 | 李视辉 | Sterilization composition containing zoxamide and metalaxyl and application |
| EP2962568A1 (en) * | 2014-07-01 | 2016-01-06 | Basf Se | Mixtures comprising a bacillus amyliquefaciens ssp. plantarum strain and a pesticide |
-
2016
- 2016-06-27 CN CN201610509313.9A patent/CN106035379A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1228255A (en) * | 1998-01-27 | 1999-09-15 | 罗姆和哈斯公司 | Fungicidal compositions containing N-acetonylbenzamides |
| CN102480965A (en) * | 2009-06-24 | 2012-05-30 | 拜耳作物科学公司 | Combinations of fungicidally active yeast and fungicides |
| CN101647435A (en) * | 2009-07-21 | 2010-02-17 | 深圳诺普信农化股份有限公司 | Synthetical sterilization composition |
| CN101999356A (en) * | 2010-11-26 | 2011-04-06 | 惠州市中迅化工有限公司 | Sterilization composition containing fluopicolide and metalaxyl and application thereof |
| CN102246760A (en) * | 2011-04-30 | 2011-11-23 | 江西海科瑞特作物科学有限公司 | Bactericidal composition containing fluopicolide and metalaxyl |
| CN102925393A (en) * | 2012-11-07 | 2013-02-13 | 中国烟草总公司郑州烟草研究院 | Tobacco endophyte resisting meloidogyne and tobacco phytophthora nicotianae and application thereof |
| CN103004819A (en) * | 2012-11-30 | 2013-04-03 | 王学权 | Fluopicolide-containing bactericide composition |
| CN103651353A (en) * | 2013-12-18 | 2014-03-26 | 李视辉 | Sterilization composition containing zoxamide and metalaxyl and application |
| EP2962568A1 (en) * | 2014-07-01 | 2016-01-06 | Basf Se | Mixtures comprising a bacillus amyliquefaciens ssp. plantarum strain and a pesticide |
Non-Patent Citations (3)
| Title |
|---|
| 李斌 等: "烟草黑胫病化学防治研究进展", 《广西农业科学》 * |
| 汪汉成 等: "烟草黑胫病化学防治的历史与现状", 《中国烟草学报》 * |
| 牟文君 等: "烟草疫霉对六种杀菌剂的敏感性测定", 《中国植物病理学会2015年学术年会论文集》 * |
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