CN106106533A - A kind of bacterium drug composition, preparation and application thereof containing acid amide fungicides and biocontrol microorganisms - Google Patents
A kind of bacterium drug composition, preparation and application thereof containing acid amide fungicides and biocontrol microorganisms Download PDFInfo
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- CN106106533A CN106106533A CN201610493553.4A CN201610493553A CN106106533A CN 106106533 A CN106106533 A CN 106106533A CN 201610493553 A CN201610493553 A CN 201610493553A CN 106106533 A CN106106533 A CN 106106533A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
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Abstract
The invention discloses a kind of bacterium drug composition, preparation and application thereof containing acid amide fungicides and biocontrol microorganisms, belong to plant protection and Pesticide Science field.This bacterium drug composition is made up of acid amide fungicides (especially carboxylic acid amide series bactericidal agent) and biocontrol microorganisms mycopowder, by quality ratio, and acid amide fungicides: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g.Biocontrol microorganisms is preferably bacillus amyloliquefaciens ZY 9 13, deposit number: CGMCC No.6162.This bacterium drug composition has excellent prophylactic-therapeutic effect to tobacco root-knot, black shank, preventive effect is significantly better than the single chemical bactericide of use or bacillus amyloliquefaciens biocontrol agent, and the drug-fast generation of chemical agent can be delayed, it is particularly well-suited to the preventing and treating of field tobacco balck shank.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing acid amide fungicides and biocontrol microorganisms, also relate to comprise this bacterium
The preparation of drug composition and application thereof, belong to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, wherein the pesticide such as metalaxyl be produce in use the widest
General medicament.Metalaxyl is phenylamide systemic fungicide, and the mid-80, at China's production and application, is prevented in oomycetes disease
Playing an important role in controlling, its mechanism of action is the polymerase activity of suppression pathogen, and RNA interfering synthesizes.But along with production
In commonly used, its resistance problem occurs the most therewith, current Different Kinds of Pathogens oomycetes all have to metalaxyl produce resistance is correlated with
Report, exploitation pharmaceutical preparations is as the replacement of metalaxyl or rotation medicament, and it is particularly important to formulate rational Strategy of resistance management.
The compounding consumption that can reduce a kind of microbiocidal composition of chemical agent, delays drug-fast generation, reduces production cost simultaneously, be
One of important measures in control of plant disease.
Carboxylic acid amide series bactericidal agent is as the surrogate-data technique of phenyl amide series bactericidal agent, and its novel structure, to cause of disease oomycetes
Having the bacteriostatic activity of excellence, main suppression pathogen stops spore and sporangial sprouting, germ tube elongation and mycelial growth (recklessly
Swallow. the resistance risk of dimethomorph is studied [D] by tobacco black shank bacterium. Shandong Agricultural University, 2007).Wherein dimethomorph is
The cinnamamide series bactericidal agent that the eighties in last century is researched and developed by American Cyanamid Co., to the ovum caused by Peronospora, Phytophthora etc.
Fungus diseases has protection and the therapeutic effect of excellence, affects normally forming of cell wall by suppression pathogen cellulosic electrode
(Cui Xiaolan, Meng Qingxiao, Bi Yang, etc. sensitivity base-line and the indoor drug resistant mutants of dimethomorph are studied [J] by Phytophthora capsici.
Plant Pathology, 2009).Fluopicolide is the Benzoylamide series bactericidal agent researched and developed by Beyer Co., Ltd, efficient to cause of disease oomycetes,
Reaching bactericidal effect by acting on the spectrin of cell membrane, the mechanism of action of its uniqueness makes itself and the equal nothing of various sterilization agent
Mutual Drug resistance (roc. fluopicolide and the co-toxicity of pyraclostrobin Phytophthora infestans and mixture preparation research
[D]. Agricultural University Of Hebei, 2013).
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant at present
Report and patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, balck shank
Antibacterial, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, the two
Weight ratio be 80:1～1:80, it is adaptable to the plant disease that caused by pathogenic fungi of preventing and treating, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of thiophene bacterium Han benzene
The bactericidal pesticide composition of amine, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, said composition
Can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide a kind of bacterium drug composition containing acid amide fungicides and biocontrol microorganisms, said composition pair
Tobacco root-knot, black shank have the prophylactic-therapeutic effect of excellence, and preventive effect is significantly better than and uses single amide-type sterilization
Agent or biocontrol microorganisms microbial inoculum, and the drug-fast generation of chemical agent can be delayed.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffers the application in preventing and treating black shank of a kind of above-mentioned bacterium medicine preparation.
In order to realize object above, the technical solution adopted in the present invention is:
A kind of bacterium drug composition, is made up of acid amide fungicides and biocontrol microorganisms mycopowder, and by quality ratio, amide-type sterilizes
Agent: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Described acid amide fungicides includes carboxylic acid amide series bactericidal agent, phenyl amide series bactericidal agent and benzamides sterilization
Agent.
Chemically dividing in structure, carboxylic acid amide series bactericidal agent includes that again cinnamamide series bactericidal agent is (such as dimethomorph, fluorine
Morpholine, pyrimorph), mandelic acidamide series bactericidal agent (such as mandipropamid) and valine amide carbamate fungicide (as
Iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal) three chemical subgroups (inteilectual Zhu, Lu Xiaohong, Chen Lei, etc. carboxylic
Acid amide-type (CAAs) antibacterial progress [J]. Pesticide Science journal, 2010,12 (1): 1-12).
Described phenyl amide series bactericidal agent (PAFs) include again acylalaninies series bactericidal agent (as metalaxyl, Metalaxyl-M,
M 9834, essence M 9834, furalaxyl), butyrolactone series bactericidal agent (such as fenfuram) and oxazolidone series bactericidal agent (as white in disliked
Spirit) (Wang Hancheng, Li Wenhong, Feng Yonggang, etc. the history of black shank chemical prevention and present situation [J]. Chinese tobacco journal,
2011,17(5):96-102).Concrete, it is selected from metalaxyl, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, first furan acyl
One or more in amine, Wakil etc..
Any one or more that described Benzoylamide series bactericidal agent is selected from fluopicolide, zoxamide etc..
Preferably, bacterium drug composition is made up of carboxylic acid amide series bactericidal agent and biocontrol microorganisms mycopowder.Carboxylic acid amide series bactericidal agent
Specifically be selected from dimethomorph, flumorph, pyrimorph, mandipropamid, iprovalicarb, benzene metsulfovax, different benzene metsulfovax,
One or more in valiphenal etc..As individually selected flumorph, benzene metsulfovax or mandipropamid, or select alkene acyl
Morpholine and mandipropamid, pyrimorph and iprovalicarb, the combination of different benzene metsulfovax and mandipropamid, also or select fluorine
Morpholine, benzene metsulfovax and the combination of mandipropamid.Compare the single a kind of antibacterial of use, composite bactericide (two kinds and more than
Antibacterial with the use of) there is summation action or potentiation.Further, composite bactericide (two kinds and more than) is combined with biocontrol microorganisms
The prevention effect to tobacco root-knot, black shank etc. is used to be better than being used alone chemical bactericide or biocontrol microorganisms bacterium
Agent.Meanwhile, in composite bactericide, each component proportion can arbitrarily be chosen, if dimethomorph and mandipropamid are according to mass ratio 1
～10:1～10 compounds, and for example dimethomorph is multiple according to mass ratio 1～10:1～10:1～10 with benzene metsulfovax, mandipropamid
Join, etc..Owing to combining form is various, do not enumerate.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Wherein, biocontrol microorganisms is excellent
Elect bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13 as, deposit number: CGMCC No.6162,
The preservation time: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Unit address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can
Suppression tobacco black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of anti-root knot of holding concurrently
The Nicotiana tabacum L. endophyte of nematicide and Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, carboxylic acid amide
Series bactericidal agent, phenyl amide series bactericidal agent and Benzoylamide series bactericidal agent are all to bacillus amyloliquefaciens (CGMCC No.6162)
Growth effect faint, the compatibility between chemical bactericide and bacillus amyloliquefaciens is good, can carry out bacterium medicine combination.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is by acid amide fungicides and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Becoming, tobacco root-knot, black shank are had excellent prophylactic-therapeutic effect, preventive effect is significantly better than and uses single amide-type
Antibacterial or bacillus amyloliquefaciens biocontrol agent, and the drug-fast generation of chemical agent can be delayed, it is particularly well-suited to field cigarette
The preventing and treating of grass balck shank.Wherein, carboxylic acid amide series bactericidal agent is inhibited to the synthesis of cause of disease oomycetes cell wall cellulose, benzene
Base acid amide fungicides can suppress the activity of RNA polymerase in pathogen, and the fluopicolide of benzamides, zoxamide can divide
Not spectrin and suppression mitochondrion β-tubulin by acting on cell membrane assembles and reaches bactericidal effect.This three class above-mentioned
Antibacterial can respectively with bacillus amyloliquefaciens with the use of, the prevention effect to tobacco root-knot, black shank etc.
More preferably.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of dimethomorph and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, dimethomorph: bacillus amyloliquefaciens mycopowder=1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of flumorph and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, flumorph: bacillus amyloliquefaciens mycopowder=1:6, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 3
Bacterium drug composition, is made up of pyrimorph and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, pyrimorph: bacillus amyloliquefaciens mycopowder=10:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 4
Bacterium drug composition, is made up of mandipropamid and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with quality
Than meter, mandipropamid: bacillus amyloliquefaciens mycopowder=5:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 5
Bacterium drug composition, is made up of iprovalicarb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, iprovalicarb: bacillus amyloliquefaciens mycopowder=1:10, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 6
Bacterium drug composition, is made up of benzene metsulfovax and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, benzene metsulfovax: bacillus amyloliquefaciens mycopowder=3:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 7
Bacterium drug composition, is made up of metalaxyl and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, metalaxyl: bacillus amyloliquefaciens mycopowder=1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 8
Bacterium drug composition, is made up of M 9834 and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, M 9834: bacillus amyloliquefaciens mycopowder=7:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 9
Bacterium drug composition, is made up of Metalaxyl-M and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, Metalaxyl-M: bacillus amyloliquefaciens mycopowder=1:10, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 10
Bacterium drug composition, is made up of fenfuram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fenfuram: bacillus amyloliquefaciens mycopowder=1:5, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 11
Bacterium drug composition, is made up of Wakil and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, Wakil: bacillus amyloliquefaciens mycopowder=10:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 12
Bacterium drug composition, is made up of fluopicolide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluopicolide: bacillus amyloliquefaciens mycopowder=1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 13
Bacterium drug composition, is made up of zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, zoxamide: bacillus amyloliquefaciens mycopowder=5:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 14
Bacterium drug composition, by fluopicolide, zoxamide and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder group
Become, by quality ratio, fluopicolide: zoxamide: bacillus amyloliquefaciens mycopowder=1:1:8, in mycopowder, viable count is not less than
106cfu/g。
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: dimethomorph 40 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), dodecyl sodium sulfate 5 parts, calcium lignosulfonate 5
Part, sucrose 8 parts, 12 parts of kieselguhr；Preparation method is: accurately takes each raw material, mix homogeneously according to mass fraction, obtains wettability
Powder.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: pyrimorph 20 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 10 parts6Cfu/g), calcium lignosulfonate 2 parts, dodecyl sodium sulfate 2
Part, sodium lauryl sulphate 3 parts, sucrose 3 parts, white carbon 10 parts；Preparation method is with embodiment 15.
Embodiment 17
Bacterium medicine preparation, is made up of the raw material of following mass fraction: mandipropamid 10 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 25 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 5 parts, pulls open 3 parts of powder, forms sediment
2 parts of powder, 2 parts of sodium sulfate, xanthan gum 3 parts, sucrose 5 parts, bentonite 10 parts, 20 parts of kieselguhr；Preparation method is: according to mass parts
Number accurately takes each raw material, pelletize after mix homogeneously, is dried, sieves, obtains suspending agent.
Embodiment 18
Bacterium medicine preparation, is made up of the raw material of following mass fraction: flumorph 10 parts, benzene metsulfovax 10 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 13 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 3 parts,
Pull open 5 parts of powder, 3 parts of sodium sulfate, xanthan gum 5 parts, sucrose 5 parts, Kaolin 17 parts；Preparation method is with embodiment 17.
Embodiment 19
Bacterium medicine preparation, is made up of the raw material of following mass fraction: iprovalicarb 18 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 20 parts6Cfu/g), sodium lignin sulfonate 5 parts, nekal 2
Part, dodecylbenzene sodium sulfonate 3 parts, polyvinylpyrrolidone 2 parts, starch 2 parts, sucrose 5 parts, 28 parts of kieselguhr；Preparation method
For: accurately take each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtain water dispersion granule.
Embodiment 20
Bacterium medicine preparation, is made up of the raw material of following mass fraction: different benzene metsulfovax 5 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 35 parts6Cfu/g), sodium lignin sulfonate 5 parts, nekal 2
Part, dodecylbenzene sodium sulfonate 3 parts, polyvinylpyrrolidone 2 parts, sucrose 5 parts, Kaolin 18 parts；The same embodiment of preparation method
19。
In other embodiments of the invention, after also two kinds and above carboxylic acid amide series bactericidal agent can being compounded, then with
Biocontrol microorganisms mycopowder combines or makes bacterium medicine preparation.
Embodiment 21
Bacterium medicine preparation, is made up of the raw material of following mass fraction: furalaxyl 50 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 10 parts6Cfu/g), calcium lignosulfonate 5 parts, sodium lauryl sulphate 6
Part, sucrose 4 parts, white carbon 10 parts, 15 parts of kieselguhr；Preparation method is: accurately take each raw material, mistake after pulverizing according to mass fraction
Sieve, mix homogeneously, obtain wettable powder.
Embodiment 22
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fenfuram 40 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 4 parts6Cfu/g), dodecyl sodium sulfate 8 parts, Laurel alcohol radical sodium sulfate 8
Part, sodium lauryl sulphate 6 parts, ethyl cellulose 5 parts, sucrose 7 parts, 10 parts of kieselguhr, attapulgite 12 parts；Preparation method is same
Embodiment 21.
Embodiment 23
Bacterium medicine preparation, is made up of the raw material of following mass fraction: essence M 9834 6 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 48 parts6Cfu/g), dodecyl sodium sulfate 5 parts, Laurel alcohol radical sodium sulfate
8 parts, calcium lignosulfonate 3 parts, ethyl cellulose 8 parts, sucrose 5 parts, Kaolin 15 parts；Preparation method is with embodiment 21.
Embodiment 24
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Metalaxyl-M 10 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 45 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 4 parts, pulls open 2 parts of powder, forms sediment
3 parts of powder, 1 part of sodium sulfate, xanthan gum 2 parts, sucrose 6 parts, bentonite 12 parts, 15 parts of kieselguhr；Preparation method is: according to mass parts
Number accurately takes each raw material, pelletize after mix homogeneously, is dried, sieves, obtains suspending agent.
Embodiment 25
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 50 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 10 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 2 parts, pulls open 6 parts of powder, sulfur
4 parts of sodium of acid, xanthan gum 7 parts, sucrose 3 parts, Kaolin 18 parts；Preparation method is with embodiment 24.
Embodiment 26
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 25 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 25 parts6Cfu/g), sodium lignin sulfonate 8 parts, nekal 5
Part, dodecylbenzene sodium sulfonate 6 parts, polyvinylpyrrolidone 5 parts, starch 4 parts, sucrose 6 parts, 16 parts of kieselguhr；Preparation method
For: accurately take each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtain water dispersion granule.
Embodiment 27
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fenfuram 20 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 35 parts6Cfu/g), polyvinylpyrrolidone 6 parts, dodecylphenol polyoxy
Vinyl Ether 5 parts, dodecylbenzene sodium sulfonate 5 parts, 6 parts of oxirane, ethyl cellulose 8 parts, attapulgite 10 parts；Preparation method
With embodiment 26.
In other embodiments of the invention, also can by after bactericide compounded to two kinds and above phenylamide, then with
Biocontrol microorganisms mycopowder combines or makes bacterium medicine preparation.
Embodiment 28
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 35 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), dodecyl sodium sulfate 6 parts, calcium lignosulfonate 8
Part, sucrose 8 parts, 20 parts of kieselguhr；Preparation method is: accurately takes each raw material, mix homogeneously according to mass fraction, obtains wettability
Powder.
Embodiment 29
Bacterium medicine preparation, is made up of the raw material of following mass fraction: zoxamide 10 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 45 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 10 parts, pull open 5 parts of powder,
Starch 3 parts, 2 parts of sodium sulfate, xanthan gum 5 parts, sucrose 10 parts, bentonite 15 parts, 20 parts of kieselguhr；Preparation method is: according to matter
Amount number accurately takes each raw material, pelletize after mix homogeneously, is dried, sieves, obtains suspending agent.
Embodiment 30
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluopicolide 15 parts, zoxamide 15 parts, solves starch bud
(in mycopowder, viable count is not less than 10 to spore bacillus (CGMCC No.6162) mycopowder 10 parts6Cfu/g), dodecyl sodium sulfate 5 parts,
Calcium lignosulfonate 7 parts, sucrose 9 parts, 22 parts of kieselguhr；Preparation method is with embodiment 28.
In other embodiments of the invention, also can by after bactericide compounded to two kinds and above benzamides, then with
Biocontrol microorganisms mycopowder combines or makes bacterium medicine preparation.
In the various embodiments described above, bacillus amyloliquefaciens (CGMCC No.6162) mycopowder is with reference to patent (notification number
CN102925393B) in detailed description of the invention, method is prepared as wettable powder.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In process group, the former medicine of carboxylic acid amide series bactericidal agent (not adding bacillus amyloliquefaciens) is 0.25 for examination concentration
μ g/mL, phenylamide bactericide original drug (not adding bacillus amyloliquefaciens) is 0.05 μ g/mL for examination concentration, Benzoylamide
The former medicine of series bactericidal agent (not adding bacillus amyloliquefaciens) is 0.15 μ g/mL for examination concentration, bacillus amyloliquefaciens (CGMCC
No.6162) concentration of bacteria suspension is 1010Cfu/mL, bacterium drug composition (all kinds of bactericide original drug corresponding concentration+solution starch spore
Bacillus (CGMCC No.6162) 1010Cfu/mL), compounding form see table 1～3.Diameter 5mm bacterium dish is placed in the Herba bromi japonici of pastille
Culture medium central authorities, every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses decussation method to measure colony diameter, calculates
The different disposal suppression ratio to tobacco black shank bacterium, evaluates the bacterium drug composition inhibitory action to black shank bacterium, and result see table 1
～3.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
Table 1 carboxylic acid amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 2 phenyl amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 3 Benzoylamide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
From table 1～3 it can be seen that compare the suppression to tobacco black shank bacterium of chemical bactericide or bacillus amyloliquefaciens
Effect, bacterium drug composition shows synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculating tobacco black shank bacterium by greenhouse, and spray bacterium medicine preparation prepared by embodiment 15～30, concrete grammar is such as
Under: the method with reference to Dong Yu prepares bacterium paddy inoculum, is added by the bacterium cake of 20 diameter 5mm in the millet of 500g sterilizing, 28 DEG C
Cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. the Chinese Academy of Agricultural Sciences,
2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum, treats that tobacco seedlings is long
During to 6～7 true leaves, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, and each process 5 strain tobacco seedlings, not apply
Medicament, add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium paddy 10g/ in cigarette strain root after dispenser 3d
Strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 4.In table 4,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 50% dimethomorph
Wettable powder is purchased from BASF AG, and 68% essence first frost MnZn water dispersible granules is purchased from first just reaching the limited public affairs of crop protection
Department, 687.5g/L Yin Fali (fluorine bacterium Propamocarb) is purchased from Beyer Co., Ltd, bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum
Prepare with reference to method in patent (notification number CN102925393B) detailed description of the invention, be more than matched group.
The table 4 bacterium medicine preparation prevention effect to black shank
From table 4, it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 15～30,
Preventive effect reaches more than 80%, is better than commercially available chemical agent and bacillus amyloliquefaciens microbial inoculum, and the preventing and treating for balck shank provides newly
Type alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (10)
1. bacterium drug composition, it is characterised in that: it is made up of acid amide fungicides and biocontrol microorganisms mycopowder, by quality ratio, amide-type
Antibacterial: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described acid amide fungicides includes carboxylic acyloxy amine
Antibacterial, phenyl amide series bactericidal agent, Benzoylamide series bactericidal agent.
Bacterium drug composition the most according to claim 2, it is characterised in that: described carboxylic acid amide series bactericidal agent includes cinnamoyl
Amine antibacterial, mandelic acidamide series bactericidal agent, valine amide carbamate fungicide.
Bacterium drug composition the most according to claim 3, it is characterised in that: described carboxylic acid amide series bactericidal agent is selected from alkene acyl
Any in quinoline, flumorph, pyrimorph, mandipropamid, iprovalicarb, benzene metsulfovax, different benzene metsulfovax, valiphenal
One or more.
Bacterium drug composition the most according to claim 2, it is characterised in that: described phenyl amide series bactericidal agent is selected from first frost
One or more in spirit, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram, Wakil.
Bacterium drug composition the most according to claim 2, it is characterised in that: described Benzoylamide series bactericidal agent is selected from fluorine pyrrole bacterium
Any one or two kinds in amine, zoxamide.
7. according to the bacterium drug composition according to any one of claim 1～6, it is characterised in that: described biocontrol microorganisms is for solving starch
Bacillus cereus ZY-9-13, deposit number: CGMCC No.6162.
8. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of claim 1～7.
9. as according to any one of claim 1～7 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
10. bacterium medicine preparation application in preventing and treating root knot nematode in tobacco and/or black shank as claimed in claim 8.
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Application publication date: 20161116 |