CN109820005A - A kind of antibacterial insect prevention ecological system formulation of biology and the preparation method and application thereof - Google Patents
A kind of antibacterial insect prevention ecological system formulation of biology and the preparation method and application thereof Download PDFInfo
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- CN109820005A CN109820005A CN201910215886.4A CN201910215886A CN109820005A CN 109820005 A CN109820005 A CN 109820005A CN 201910215886 A CN201910215886 A CN 201910215886A CN 109820005 A CN109820005 A CN 109820005A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses antibacterial insect prevention ecological system formulations of a kind of biology and its preparation method and application.It is characterized in that it is a kind of ecological system formulation for integrating microorganism, organic active carrier, organic nutrition, micro-element nutrition, auxiliary insect prevention plant extracts and adapting to microorganism growth, it is made of following portions by weight material: 1~3 part of Chinese medicine；1~3 part of fruits and vegetables；1~3 part of vegetable castoff；10~15 parts of water；0.3~0.5 part of sugar；0.01~0.02 part of function bacterium；0.05~0.1 part of carbonization organic matter；0.005~0.01 part of wood vinegar；0.01~0.02 part of mineral element；0.0001~0.0002 part of agricultural grade chitosan oligosaccharide；0.01~0.02 part of agricultural grade microelement；0.01~0.02 part of plant extracts.The preparation can base apply and spray, in green cucumber fungistatic effect relatively control improve 75%, trialeurodes vaporariorum reduce 60%.
Description
Technical field
The present invention relates to antibacterial insect prevention ecological system formulations of biology and the preparation method and application thereof, belong to microbial fermentation
And plant protection art.
Background technique
In traditional agricultural production, most of peasant households are limited by technical level and management cost and are all sprayed using chemical agent
It applies, the drawbacks of chemical pesticide is pollution it will be apparent that include the residual of agricultural product pesticide, to soil and air environment and right
Agricultural producer and the harm of agricultural product consumer health etc..It is micro- to improve the safety of agricultural production and the quality of agricultural product
The antibacterial insect prevention preparation of biology comes into being, this is also necessarily becoming under green agriculture and Agricultural product without pollution idea
Gesture.Being made using microorganism either microbial metabolic products and can be used as the active material of Pesticide use is exactly the antibacterial insect prevention of biology
Preparation.
Microorganism formulation refers to that " a kind of specific products containing viable microbial, is applied in agricultural production, crop can obtain spy
Fixed fertilizer effect, in the production of this effect, viable microbial or its metabolite in product are played a key role ",
Because microorganism formulation is that a kind of lived cell living is playing an important role, effect will receive the serious shadow of environment
It rings, such as the influence of nutrient environment, soil environment, indigenous microorganism and weather, therefore the promotion conference of microorganism formulation is by very
Big influence, the present invention is based on these considerations, on the basis of fully considering microorganism formulation function, are entering soil ring
Microorganism is first given before border and builds suitable nutrient environment, such as organic active carrier, organic nutrition, inorganic nutrients, micro
Element nutrition and other insect prevention vegetable matters, the ecosystem for being configured to an Adaptable growth to microorganism enter rhizosphere
Around, it breeds rapidly, forms dominant bacteria, to play the effect of real antibacterial insect prevention.
Summary of the invention
The object of the present invention is to provide antibacterial insect prevention ecological system formulations of a kind of biology and the preparation method and application thereof.
It is characterized in that it is to be separately added into the filtering liquid mixture after function bacterium is fermented by Chinese medicine, fruits and vegetables and vegetable castoff to carry out again
Carbonization organic matter, wood vinegar, natural minerals element, agricultural and chitosan oligosaccharide, agricultural grade microelement, plant are added after secondary fermentation to mention
It takes object to produce, is that a kind of collection microorganism, organic active carrier, organic nutrition, micro-element nutrition, auxiliary insect prevention plant mention
It takes object in the ecological system formulation for adapting to microorganism growth of one, can be bred rapidly around rhizosphere after, formed excellent
Gesture bacterium, to play real effect, including antibacterial insect prevention, anti-continuous cropping stimulates crop growth, improves utilization rate of fertilizer, agriculture
The effect of crop yield and quality, it is made of the fermentation material of following portions by weight:
Chinese medicine: 1~3 part；Fruits and vegetables: 1~3 part；Vegetable castoff: 1~3 part；Water: 10~15 parts；Sugar: 0.3~0.5 part；
Function bacterium (saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, healthy and free from worry wood
It is mould) 0.01~0.02 part；0.05~0.1 part of carbonization organic matter；0.005~0.01 part of wood vinegar；Natural minerals element 0.01~
0.02 part；0.0001~0.0002 part of agricultural grade chitosan oligosaccharide；0.01~0.02 part of agricultural grade microelement；Plant extracts 0.01
~0.02 part.
Function bacterium provided by the invention, its active constituent include saccharomycete (Saccharomyces cerevisiae),
Lactic acid bacteria (Lactobacillus plantarum), bacillus subtilis (Bacillus subtilis), gel-shaped gemma bar
Bacterium (Bacillus mucilaginosus), Dipel (Bacillus thuringiensis), Trichoderma harzianum
(Trichoderma harzianum) and trichodermaharzianum (Trichoderma harzianumRifai).
The saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, health
The weight ratio of peaceful trichoderma is 0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part:
0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part.
The saccharomycete can be the bacterium that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 20065
Strain；The lactic acid bacteria can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10171；
The bacillus subtilis can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 11089
Bacterial strain；The Bacillus mucilaginosus can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10013
Bacterial strain.The Dipel can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10016
Bacterial strain.The Trichoderma harzianum can be the bacterium that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30371
Strain.The trichodermaharzianum can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30167.
The Chinese medicine parts by weight refer to: Chinese prickly ash: 0.5~1 part；Azedarach: 0.5~1 part；Honeysuckle: 0.5
~1 part；Aloe: 0.5~1 part.
The fruits and vegetables parts by weight refer to: 0.5~1 part of jujube；0.5~1 part of apple；0.5~1 part of tomato；Ginger 0.5
~1 part；0.5~1 part of garlic.
The vegetable castoff parts by weight refer to: 1~2 part of tomato waste；1~2 part of capsicum waste；Cabbage
Waste: 1~2 part.
The carbonization organic matter refers to the solid carbonization formed by agricultural crop straw and rice husk by imperfect combustion
Organic matter, market are purchased.
The wood vinegar refers to passing through the gas aqueous solution that imperfect combustion is formed, city by agricultural crop straw and rice husk
It purchases field.
The natural minerals element refers to ooze mineral nutrition, and market is purchased.
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased.
The plant extracts refers to matrine, and market is purchased.
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.003 part；Copper sulphate:
0.001~0.003 part；Zinc sulfate: 0.001~0.003 part；Manganese sulfate: 0.001~0.003 part；Molybdenum: 0.001~0.003 part；
Boron: 0.001~0.003 part, market is purchased.
The present invention further discloses the production and application method of the antibacterial insect prevention ecological system formulation of biology, feature exists
It is carried out in by following step:
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5~1 part；Azedarach: 0.5~1 part；Honeysuckle: 0.5
~1 part；Aloe: 0.5~1 part；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of jujube；0.5~1 part of apple；0.5~1 part of tomato；Ginger
0.5~1 part；0.5~1 part of garlic；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of tomato waste；1~2 part of capsicum waste；
The waste of cabbage: 1~2 part；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month；
(7) filter mixed fermentation: filtered fluid is mixed by 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after conjunction；
(8) carbonization organic matter is added: 0.05~0.1 part being added after filtering mixed fermentation；
(9) wood vinegar is added: 0.005~0.01 part being added after filtering mixed fermentation；
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation；
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation；
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation；
(13) plant extracts is added: 0.01~0.02 part of plant extracts being added after filtering mixed fermentation；
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L；
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre；Spray
It applies, is sprayed on blade.
The present invention further discloses application and effect of the antibacterial insect prevention ecological system formulation of biology on green cucumber
Fruit.The antibacterial insect prevention ecological system formulation of biology of the invention is applied after on green cucumber, and test result shows more right
All good according to protection effect, for preventive effect up to 75%, trialeurodes vaporariorum incidence reduces 60%.
Chinese medicine Chinese prickly ash, azedarach, honeysuckle, aloe, primary efficacy used in the present invention are as follows:
Chinese prickly ash, azedarach, aloe have effects that desinsection, and honeysuckle has effects that sterilization.
The antibacterial insect prevention ecological system formulation of biology provided by the invention can antibacterial insect prevention, anti-continuous cropping, stimulation crops are raw
It is long, improve the effect of utilization rate of fertilizer, crop yield and quality
Positive effect possessed by the antibacterial insect prevention ecological system formulation of biology disclosed by the invention and application compared with prior art
Fruit is:
(1) beneficial microbe is in being applied in the form of an organic ecological system onto soil or blade face；
(2) growth and breeding ability is high after beneficial microbe is manured into soil, and plays insect prevention and disease-resistant effect to plant growth.
(3) safety is increased, unharmful substance improves the safety of agricultural product.
(4) raw material sterilization is sterilized and is placed twice using 200ppm chlorine dioxide (purchasing in market) 5 hours intermittences
24 hours methods, it is at low cost, it is harmless, conducive to the progress of fermenting raw materials, and guarantee the qualification of product.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Embodiment 1: the production and application method of the antibacterial insect prevention ecological system formulation of biology
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5 part；Azedarach: 0.5 part；Honeysuckle: 0.5 part；Reed
Luxuriant growth: 0.5 part；Water: 3 parts；Sugar: 0.3 part；0.007 part of function bacterium, wherein saccharomycete: 0.001 part；Lactic acid bacteria: 0.001 part；It is withered
Careless bacillus: 0.001 part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Kazakhstan thatch rasp berry: 0.001
Part；Healthy and free from worry rasp berry: 0.001 part；
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5 part of jujube；0.5 part of apple；0.5 part of tomato；0.5 part of ginger；Garlic
0.5 part；Water: 3 parts；Sugar: 0.3 part；0.007 part of function bacterium, wherein saccharomycete: 0.001 part；Lactic acid bacteria: 0.001 part；Withered grass bud
Spore bacillus: 0.001 part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Kazakhstan thatch rasp berry: 0.001 part；Health
Peaceful rasp berry: 0.001 part；
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1 part of tomato waste；1 part of capsicum waste；Cabbage
Waste: 1 part；Water: 3 parts；Sugar: 0.3 part；0.007 part of function bacterium, wherein saccharomycete: 0.002 part；Lactic acid bacteria: 0.002 part；
Bacillus subtilis: 0.001 part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Kazakhstan thatch rasp berry: 0.001
Part；Healthy and free from worry rasp berry: 0.001 part；
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month；
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing；
(8) carbonization organic matter is added: 0.01 part being added after filtering mixed fermentation；
(9) wood vinegar is added: 0.01 part being added after filtering mixed fermentation；
(10) natural minerals element is added: 0.02 part of natural minerals element being added after filtering mixed fermentation；
(11) agricultural grade chitosan oligosaccharide is added: 0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation；
(12) agricultural grade microelement is added: 0.02 part of agricultural grade microelement being added after filtering mixed fermentation；
(13) plant extracts is added: 0.01 part of plant extracts being added after filtering mixed fermentation；
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L；
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre；Spray
It applies, is sprayed on blade.
Embodiment 2: the production and application method of the antibacterial insect prevention ecological system formulation of biology
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 1 part；Azedarach: 1 part；Honeysuckle: 1 part；Aloe: 1
Part；Water: 5 parts；Sugar: 0.5 part；0.01 part of function bacterium, wherein saccharomycete: 0.003 part；Lactic acid bacteria: 0.002 part；Bacillus subtilis
Bacterium: 0.001 part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Kazakhstan thatch rasp berry: 0.001 part；Healthy and free from worry wood
The certain kind of berries: 0.001 part；
(2) biological fruit and vegetable ferment fermentation raw material and formula: 1 part of jujube；1 part of apple；1 part of tomato；1 part of ginger；1 part of garlic；Water: 5
Part；Sugar: 0.5 part；0.01 part of function bacterium, wherein saccharomycete: 0.003 part；Lactic acid bacteria: 0.002 part；Bacillus subtilis: 0.001
Part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Kazakhstan thatch rasp berry: 0.001 part；Healthy and free from worry rasp berry: 0.001
Part；
(3) vegetable castoff biologic ferment fermentation raw material and formula: 2 parts of tomato waste；0.5 part of capsicum waste；Circle is white
The waste of dish: 0.5 part；Water: 5 parts；Sugar: 00.5 part；0.01 part of function bacterium, wherein saccharomycete: 0.003 part；Lactic acid bacteria:
0.002 part；Bacillus subtilis: 0.001 part；Bacillusmusilaginosiengineering: 0.001 part；Dipel: 0.001 part；Breathe out thatch wood
The certain kind of berries: 0.001 part；Healthy and free from worry rasp berry: 0.001 part；
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month；
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing；
(8) carbonization organic matter is added: 0.06 part being added after filtering mixed fermentation；
(9) wood vinegar is added: 0.006 part being added after filtering mixed fermentation；
(10) natural minerals element is added: 0.016 part of natural minerals element being added after filtering mixed fermentation；
(11) agricultural grade chitosan oligosaccharide is added: 0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation；
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation；
(13) plant extracts is added: 0.02 part of plant extracts being added after filtering mixed fermentation；
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L；
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre；Spray
It applies, is sprayed on blade.
Embodiment 3: the field trial of the antibacterial insect prevention ecological system formulation of biology
The antibacterial insect prevention ecological system formulation of biology can antibacterial insect prevention, anti-continuous cropping stimulates crop growth, improves fertilizer utilization
The effect of rate, crop yield and quality.
, test site and varieties of plant
Test site is the Tianjin Xiqing District village Dang Cheng, and test variety is cucumber.
, field design
Totally 2 processing, 3 repetitions of each processing, plot area 16m2。
Processing 1: the antibacterial insect prevention ecological system formulation of biology；Processing 2: control (does not spray the antibacterial insect prevention ecological system of biology
Agent).
, test result
Application effect of the 1 antibacterial insect prevention ecological system formulation of biology of table in green cucumber
Test result shows antibacterial insect prevention ecological system formulation preventable disease during green cucumber of biology, relatively imitates to diseases prevention
Fruit is all good, and for preventive effect up to 75%, trialeurodes vaporariorum incidence reduces 60%.
Claims (4)
1. a kind of antibacterial insect prevention ecological system formulation of biology, it is characterized in that being made of the raw material of following portions by weight:
Chinese medicine: 1~3 part；Fruits and vegetables: 1~3 part；Vegetable castoff: 1~3 part；Water: 10~15 parts；Sugar: 0.3~0.5 part；
Function bacterium (saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, healthy and free from worry wood
It is mould) 0.01~0.02 part；0.05~0.1 part of carbonization organic matter；0.005~0.01 part of wood vinegar；Natural minerals element 0.01~
0.02 part；0.0001~0.0002 part of agricultural grade chitosan oligosaccharide；0.01~0.02 part of agricultural grade microelement；Plant extracts 0.01
~0.02 part；
The parts by weight of the function bacterium refer to: saccharomycete: 0.001~0.005 part；Lactic acid bacteria: 0.001~0.005 part；It is withered
Careless bacillus: 0.001~0.005 part；Bacillusmusilaginosiengineering: 0.001~0.005 part；Dipel: 0.001~
0.005 part；Kazakhstan thatch rasp berry: 0.001~0.005 part；Healthy and free from worry rasp berry: 0.001~0.005 part；
The Chinese medicine parts by weight refer to: Chinese prickly ash: 0.5~1 part；Azedarach: 0.5~1 part；Honeysuckle: 0.5~1
Part；Aloe: 0.5~1 part；
The fruits and vegetables parts by weight refer to: 0.5~1 part of jujube；0.5~1 part of apple；0.5~1 part of tomato；Ginger 0.5~1
Part；0.5~1 part of garlic；
The vegetable castoff parts by weight refer to: 1~2 part of tomato waste；1~2 part of capsicum waste；Cabbage
Waste: 1~2 part；
The carbonization organic matter refers to that the solid carbonization formed by agricultural crop straw and rice husk by imperfect combustion is organic
Object, market are purchased；
The wood vinegar refers to passing through the gas aqueous solution that imperfect combustion is formed, market purchase by agricultural crop straw and rice husk
It sets；
The natural minerals element refers to ooze mineral nutrition, and market is purchased；
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased；
The plant extracts refers to matrine, and market is purchased；
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.003 part；Copper sulphate: 0.001~
0.003 part；Zinc sulfate: 0.001~0.003 part；Manganese sulfate: 0.001~0.003 part；Molybdenum: 0.001~0.003 part；Boron:
0.001~0.003 part.
2. the production and application method of the antibacterial insect prevention ecological system formulation of biology described in claim 1, it is characterised in that by such as
Under step carry out:
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5~1 part；Azedarach: 0.5~1 part；Honeysuckle: 0.5
~1 part；Aloe: 0.5~1 part；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of jujube；0.5~1 part of apple；0.5~1 part of tomato；Ginger
0.5~1 part；0.5~1 part of garlic；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of tomato waste；1~2 part of capsicum waste；
The waste of cabbage: 1~2 part；Water: 3~5 parts；Sugar: 0.3~0.5 part；0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part；Lactic acid bacteria: 0.0005~0.001 part；Bacillus subtilis: 0.0005~0.001 part；Gel-shaped bud
Spore bacillus: 0.0005~0.001 part；Dipel: 0.0005~0.001 part；Kazakhstan thatch rasp berry: 0.0005~0.001 part；Health
Peaceful rasp berry: 0.0005~0.001 part；
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month；
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours plus
Enter brown sugar → addition function fermenting microbe → lid lid sealing individually to ferment, fermentation time 1 month；
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing；
(8) carbonization organic matter is added: 0.05~0.1 part being added after filtering mixed fermentation；
(9) wood vinegar is added: 0.005~0.01 part being added after filtering mixed fermentation；
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation；
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation；
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation；
(13) plant extracts is added: 0.01~0.02 part of plant extracts being added after filtering mixed fermentation；
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L；
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre；Spray
It applies, is sprayed on blade.
3. application of the antibacterial insect prevention preparation of biology described in claim 1 in terms of improving cucumber anti-efficiency.
4. application of the antibacterial insect prevention preparation of biology described in claim 1 in terms of reducing cucumber trialeurodes vaporariorum incidence.
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Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Applicant after: Tianjin Academy of Agricultural Sciences Address before: 300384 microbiology laboratory, Tianjin Institute of agricultural resources and environment, 17 km away from Jinjing highway, Xiqing District, Tianjin Applicant before: TIANJIN INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT SCIENCE |
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Application publication date: 20190531 |