CN109820005A - Biological antibacterial insect-prevention ecological system preparation and preparation method and application thereof - Google Patents
Biological antibacterial insect-prevention ecological system preparation and preparation method and application thereof Download PDFInfo
- Publication number
- CN109820005A CN109820005A CN201910215886.4A CN201910215886A CN109820005A CN 109820005 A CN109820005 A CN 109820005A CN 201910215886 A CN201910215886 A CN 201910215886A CN 109820005 A CN109820005 A CN 109820005A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- added
- parts
- agricultural
- vegetables
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 235000012055 fruits and vegetables Nutrition 0.000 claims abstract description 26
- 235000013311 vegetables Nutrition 0.000 claims abstract description 23
- 239000002699 waste material Substances 0.000 claims abstract description 19
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 14
- 239000000419 plant extract Substances 0.000 claims abstract description 14
- 239000002023 wood Substances 0.000 claims abstract description 14
- 239000005416 organic matter Substances 0.000 claims abstract description 11
- 235000021419 vinegar Nutrition 0.000 claims abstract description 10
- 239000000052 vinegar Substances 0.000 claims abstract description 10
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 8
- 241000018137 Trialeurodes vaporariorum Species 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 83
- 230000004151 fermentation Effects 0.000 claims description 83
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 36
- 230000002265 prevention Effects 0.000 claims description 30
- 241000238631 Hexapoda Species 0.000 claims description 29
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims description 26
- 238000001914 filtration Methods 0.000 claims description 25
- 235000011034 Rubus glaucus Nutrition 0.000 claims description 24
- 244000235659 Rubus idaeus Species 0.000 claims description 24
- 235000009122 Rubus idaeus Nutrition 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 23
- 239000003814 drug Substances 0.000 claims description 22
- 238000009472 formulation Methods 0.000 claims description 22
- 241000235342 Saccharomycetes Species 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 18
- 235000014655 lactic acid Nutrition 0.000 claims description 18
- 239000004310 lactic acid Substances 0.000 claims description 18
- 244000063299 Bacillus subtilis Species 0.000 claims description 16
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 15
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 15
- 239000011707 mineral Substances 0.000 claims description 15
- 235000010755 mineral Nutrition 0.000 claims description 15
- 239000002994 raw material Substances 0.000 claims description 15
- 239000004155 Chlorine dioxide Substances 0.000 claims description 13
- 235000019398 chlorine dioxide Nutrition 0.000 claims description 13
- 239000004577 thatch Substances 0.000 claims description 13
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 12
- 240000003768 Solanum lycopersicum Species 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 238000003763 carbonization Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 9
- 241000205585 Aquilegia canadensis Species 0.000 claims description 8
- 241000223260 Trichoderma harzianum Species 0.000 claims description 8
- 244000089698 Zanthoxylum simulans Species 0.000 claims description 8
- 241001116389 Aloe Species 0.000 claims description 7
- 240000008067 Cucumis sativus Species 0.000 claims description 7
- 235000011399 aloe vera Nutrition 0.000 claims description 7
- 240000002234 Allium sativum Species 0.000 claims description 6
- 235000002566 Capsicum Nutrition 0.000 claims description 6
- 240000008574 Capsicum frutescens Species 0.000 claims description 6
- 244000273928 Zingiber officinale Species 0.000 claims description 6
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 6
- 244000126002 Ziziphus vulgaris Species 0.000 claims description 6
- 239000001390 capsicum minimum Substances 0.000 claims description 6
- 235000004611 garlic Nutrition 0.000 claims description 6
- 235000008397 ginger Nutrition 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 6
- 230000035764 nutrition Effects 0.000 claims description 6
- 240000007124 Brassica oleracea Species 0.000 claims description 5
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 5
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 5
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 238000002485 combustion reaction Methods 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- 239000010903 husk Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 235000019833 protease Nutrition 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims description 2
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 229940099596 manganese sulfate Drugs 0.000 claims description 2
- 239000011702 manganese sulphate Substances 0.000 claims description 2
- 235000007079 manganese sulphate Nutrition 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 229930014456 matrine Natural products 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 239000011733 molybdenum Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 12
- 230000012010 growth Effects 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 235000013619 trace mineral Nutrition 0.000 abstract 2
- 239000011573 trace mineral Substances 0.000 abstract 2
- 244000299906 Cucumis sativus var. sativus Species 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 239000002366 mineral element Substances 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000012271 agricultural production Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000009335 monocropping Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- 239000007633 bacillus mucilaginosus Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000021028 berry Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological antibacterial insect-proof ecological system preparation and a preparation method and application thereof. The ecological system preparation is characterized by integrating microorganisms, organic active carriers, organic nutrients, trace element nutrients and auxiliary insect-proof plant extracts into a whole and adapting to the growth of the microorganisms, and comprises the following materials in parts by weight: 1-3 parts of traditional Chinese medicinal materials; 1-3 parts of fruits and vegetables; 1-3 parts of vegetable waste; 10-15 parts of water; 0.3-0.5 part of sugar; 0.01-0.02 parts of functional bacteria; 0.05-0.1 part of carbonized organic matter; 0.005-0.01 part of wood vinegar; 0.01-0.02 parts of mineral elements; 0.0001-0.0002 part of agricultural chitosan oligosaccharide; 0.01-0.02 parts of agricultural trace elements; 0.01-0.02 part of plant extract. The preparation can be applied on a basal basis or sprayed, the antibacterial effect is improved by 75% compared with that of a control in cucumber planting, and the trialeurodes vaporariorum is reduced by 60%.
Description
Technical field
The present invention relates to antibacterial insect prevention ecological system formulations of biology and the preparation method and application thereof, belong to microbial fermentation
And plant protection art.
Background technique
In traditional agricultural production, most of peasant households are limited by technical level and management cost and are all sprayed using chemical agent
It applies, the drawbacks of chemical pesticide is pollution it will be apparent that include the residual of agricultural product pesticide, to soil and air environment and right
Agricultural producer and the harm of agricultural product consumer health etc..It is micro- to improve the safety of agricultural production and the quality of agricultural product
The antibacterial insect prevention preparation of biology comes into being, this is also necessarily becoming under green agriculture and Agricultural product without pollution idea
Gesture.Being made using microorganism either microbial metabolic products and can be used as the active material of Pesticide use is exactly the antibacterial insect prevention of biology
Preparation.
Microorganism formulation refers to that " a kind of specific products containing viable microbial, is applied in agricultural production, crop can obtain spy
Fixed fertilizer effect, in the production of this effect, viable microbial or its metabolite in product are played a key role ",
Because microorganism formulation is that a kind of lived cell living is playing an important role, effect will receive the serious shadow of environment
It rings, such as the influence of nutrient environment, soil environment, indigenous microorganism and weather, therefore the promotion conference of microorganism formulation is by very
Big influence, the present invention is based on these considerations, on the basis of fully considering microorganism formulation function, are entering soil ring
Microorganism is first given before border and builds suitable nutrient environment, such as organic active carrier, organic nutrition, inorganic nutrients, micro
Element nutrition and other insect prevention vegetable matters, the ecosystem for being configured to an Adaptable growth to microorganism enter rhizosphere
Around, it breeds rapidly, forms dominant bacteria, to play the effect of real antibacterial insect prevention.
Summary of the invention
The object of the present invention is to provide antibacterial insect prevention ecological system formulations of a kind of biology and the preparation method and application thereof.
It is characterized in that it is to be separately added into the filtering liquid mixture after function bacterium is fermented by Chinese medicine, fruits and vegetables and vegetable castoff to carry out again
Carbonization organic matter, wood vinegar, natural minerals element, agricultural and chitosan oligosaccharide, agricultural grade microelement, plant are added after secondary fermentation to mention
It takes object to produce, is that a kind of collection microorganism, organic active carrier, organic nutrition, micro-element nutrition, auxiliary insect prevention plant mention
It takes object in the ecological system formulation for adapting to microorganism growth of one, can be bred rapidly around rhizosphere after, formed excellent
Gesture bacterium, to play real effect, including antibacterial insect prevention, anti-continuous cropping stimulates crop growth, improves utilization rate of fertilizer, agriculture
The effect of crop yield and quality, it is made of the fermentation material of following portions by weight:
Chinese medicine: 1~3 part;Fruits and vegetables: 1~3 part;Vegetable castoff: 1~3 part;Water: 10~15 parts;Sugar: 0.3~0.5 part;
Function bacterium (saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, healthy and free from worry wood
It is mould) 0.01~0.02 part;0.05~0.1 part of carbonization organic matter;0.005~0.01 part of wood vinegar;Natural minerals element 0.01~
0.02 part;0.0001~0.0002 part of agricultural grade chitosan oligosaccharide;0.01~0.02 part of agricultural grade microelement;Plant extracts 0.01
~0.02 part.
Function bacterium provided by the invention, its active constituent include saccharomycete (Saccharomyces cerevisiae),
Lactic acid bacteria (Lactobacillus plantarum), bacillus subtilis (Bacillus subtilis), gel-shaped gemma bar
Bacterium (Bacillus mucilaginosus), Dipel (Bacillus thuringiensis), Trichoderma harzianum
(Trichoderma harzianum) and trichodermaharzianum (Trichoderma harzianumRifai).
The saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, health
The weight ratio of peaceful trichoderma is 0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part:
0.001~0.005 part: 0.001~0.005 part: 0.001~0.005 part.
The saccharomycete can be the bacterium that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 20065
Strain;The lactic acid bacteria can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10171;
The bacillus subtilis can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 11089
Bacterial strain;The Bacillus mucilaginosus can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10013
Bacterial strain.The Dipel can be that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 10016
Bacterial strain.The Trichoderma harzianum can be the bacterium that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30371
Strain.The trichodermaharzianum can be the bacterial strain that Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC 30167.
The Chinese medicine parts by weight refer to: Chinese prickly ash: 0.5~1 part;Azedarach: 0.5~1 part;Honeysuckle: 0.5
~1 part;Aloe: 0.5~1 part.
The fruits and vegetables parts by weight refer to: 0.5~1 part of jujube;0.5~1 part of apple;0.5~1 part of tomato;Ginger 0.5
~1 part;0.5~1 part of garlic.
The vegetable castoff parts by weight refer to: 1~2 part of tomato waste;1~2 part of capsicum waste;Cabbage
Waste: 1~2 part.
The carbonization organic matter refers to the solid carbonization formed by agricultural crop straw and rice husk by imperfect combustion
Organic matter, market are purchased.
The wood vinegar refers to passing through the gas aqueous solution that imperfect combustion is formed, city by agricultural crop straw and rice husk
It purchases field.
The natural minerals element refers to ooze mineral nutrition, and market is purchased.
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased.
The plant extracts refers to matrine, and market is purchased.
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.003 part;Copper sulphate:
0.001~0.003 part;Zinc sulfate: 0.001~0.003 part;Manganese sulfate: 0.001~0.003 part;Molybdenum: 0.001~0.003 part;
Boron: 0.001~0.003 part, market is purchased.
The present invention further discloses the production and application method of the antibacterial insect prevention ecological system formulation of biology, feature exists
It is carried out in by following step:
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5~1 part;Azedarach: 0.5~1 part;Honeysuckle: 0.5
~1 part;Aloe: 0.5~1 part;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of jujube;0.5~1 part of apple;0.5~1 part of tomato;Ginger
0.5~1 part;0.5~1 part of garlic;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of tomato waste;1~2 part of capsicum waste;
The waste of cabbage: 1~2 part;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) filter mixed fermentation: filtered fluid is mixed by 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after conjunction;
(8) carbonization organic matter is added: 0.05~0.1 part being added after filtering mixed fermentation;
(9) wood vinegar is added: 0.005~0.01 part being added after filtering mixed fermentation;
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) plant extracts is added: 0.01~0.02 part of plant extracts being added after filtering mixed fermentation;
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L;
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre;Spray
It applies, is sprayed on blade.
The present invention further discloses application and effect of the antibacterial insect prevention ecological system formulation of biology on green cucumber
Fruit.The antibacterial insect prevention ecological system formulation of biology of the invention is applied after on green cucumber, and test result shows more right
All good according to protection effect, for preventive effect up to 75%, trialeurodes vaporariorum incidence reduces 60%.
Chinese medicine Chinese prickly ash, azedarach, honeysuckle, aloe, primary efficacy used in the present invention are as follows:
Chinese prickly ash, azedarach, aloe have effects that desinsection, and honeysuckle has effects that sterilization.
The antibacterial insect prevention ecological system formulation of biology provided by the invention can antibacterial insect prevention, anti-continuous cropping, stimulation crops are raw
It is long, improve the effect of utilization rate of fertilizer, crop yield and quality
Positive effect possessed by the antibacterial insect prevention ecological system formulation of biology disclosed by the invention and application compared with prior art
Fruit is:
(1) beneficial microbe is in being applied in the form of an organic ecological system onto soil or blade face;
(2) growth and breeding ability is high after beneficial microbe is manured into soil, and plays insect prevention and disease-resistant effect to plant growth.
(3) safety is increased, unharmful substance improves the safety of agricultural product.
(4) raw material sterilization is sterilized and is placed twice using 200ppm chlorine dioxide (purchasing in market) 5 hours intermittences
24 hours methods, it is at low cost, it is harmless, conducive to the progress of fermenting raw materials, and guarantee the qualification of product.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Embodiment 1: the production and application method of the antibacterial insect prevention ecological system formulation of biology
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5 part;Azedarach: 0.5 part;Honeysuckle: 0.5 part;Reed
Luxuriant growth: 0.5 part;Water: 3 parts;Sugar: 0.3 part;0.007 part of function bacterium, wherein saccharomycete: 0.001 part;Lactic acid bacteria: 0.001 part;It is withered
Careless bacillus: 0.001 part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Kazakhstan thatch rasp berry: 0.001
Part;Healthy and free from worry rasp berry: 0.001 part;
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5 part of jujube;0.5 part of apple;0.5 part of tomato;0.5 part of ginger;Garlic
0.5 part;Water: 3 parts;Sugar: 0.3 part;0.007 part of function bacterium, wherein saccharomycete: 0.001 part;Lactic acid bacteria: 0.001 part;Withered grass bud
Spore bacillus: 0.001 part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Kazakhstan thatch rasp berry: 0.001 part;Health
Peaceful rasp berry: 0.001 part;
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1 part of tomato waste;1 part of capsicum waste;Cabbage
Waste: 1 part;Water: 3 parts;Sugar: 0.3 part;0.007 part of function bacterium, wherein saccharomycete: 0.002 part;Lactic acid bacteria: 0.002 part;
Bacillus subtilis: 0.001 part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Kazakhstan thatch rasp berry: 0.001
Part;Healthy and free from worry rasp berry: 0.001 part;
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing;
(8) carbonization organic matter is added: 0.01 part being added after filtering mixed fermentation;
(9) wood vinegar is added: 0.01 part being added after filtering mixed fermentation;
(10) natural minerals element is added: 0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) plant extracts is added: 0.01 part of plant extracts being added after filtering mixed fermentation;
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L;
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre;Spray
It applies, is sprayed on blade.
Embodiment 2: the production and application method of the antibacterial insect prevention ecological system formulation of biology
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 1 part;Azedarach: 1 part;Honeysuckle: 1 part;Aloe: 1
Part;Water: 5 parts;Sugar: 0.5 part;0.01 part of function bacterium, wherein saccharomycete: 0.003 part;Lactic acid bacteria: 0.002 part;Bacillus subtilis
Bacterium: 0.001 part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Kazakhstan thatch rasp berry: 0.001 part;Healthy and free from worry wood
The certain kind of berries: 0.001 part;
(2) biological fruit and vegetable ferment fermentation raw material and formula: 1 part of jujube;1 part of apple;1 part of tomato;1 part of ginger;1 part of garlic;Water: 5
Part;Sugar: 0.5 part;0.01 part of function bacterium, wherein saccharomycete: 0.003 part;Lactic acid bacteria: 0.002 part;Bacillus subtilis: 0.001
Part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Kazakhstan thatch rasp berry: 0.001 part;Healthy and free from worry rasp berry: 0.001
Part;
(3) vegetable castoff biologic ferment fermentation raw material and formula: 2 parts of tomato waste;0.5 part of capsicum waste;Circle is white
The waste of dish: 0.5 part;Water: 5 parts;Sugar: 00.5 part;0.01 part of function bacterium, wherein saccharomycete: 0.003 part;Lactic acid bacteria:
0.002 part;Bacillus subtilis: 0.001 part;Bacillusmusilaginosiengineering: 0.001 part;Dipel: 0.001 part;Breathe out thatch wood
The certain kind of berries: 0.001 part;Healthy and free from worry rasp berry: 0.001 part;
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours →
Brown sugar → addition function fermenting microbe → lid lid sealing is added individually to ferment, fermentation time 1 month;
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing;
(8) carbonization organic matter is added: 0.06 part being added after filtering mixed fermentation;
(9) wood vinegar is added: 0.006 part being added after filtering mixed fermentation;
(10) natural minerals element is added: 0.016 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) plant extracts is added: 0.02 part of plant extracts being added after filtering mixed fermentation;
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L;
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre;Spray
It applies, is sprayed on blade.
Embodiment 3: the field trial of the antibacterial insect prevention ecological system formulation of biology
The antibacterial insect prevention ecological system formulation of biology can antibacterial insect prevention, anti-continuous cropping stimulates crop growth, improves fertilizer utilization
The effect of rate, crop yield and quality.
, test site and varieties of plant
Test site is the Tianjin Xiqing District village Dang Cheng, and test variety is cucumber.
, field design
Totally 2 processing, 3 repetitions of each processing, plot area 16m2。
Processing 1: the antibacterial insect prevention ecological system formulation of biology;Processing 2: control (does not spray the antibacterial insect prevention ecological system of biology
Agent).
, test result
Application effect of the 1 antibacterial insect prevention ecological system formulation of biology of table in green cucumber
Test result shows antibacterial insect prevention ecological system formulation preventable disease during green cucumber of biology, relatively imitates to diseases prevention
Fruit is all good, and for preventive effect up to 75%, trialeurodes vaporariorum incidence reduces 60%.
Claims (4)
1. a kind of antibacterial insect prevention ecological system formulation of biology, it is characterized in that being made of the raw material of following portions by weight:
Chinese medicine: 1~3 part;Fruits and vegetables: 1~3 part;Vegetable castoff: 1~3 part;Water: 10~15 parts;Sugar: 0.3~0.5 part;
Function bacterium (saccharomycete, lactic acid bacteria, bacillus subtilis, bacillusmusilaginosiengineering, Dipel, Trichoderma harzianum, healthy and free from worry wood
It is mould) 0.01~0.02 part;0.05~0.1 part of carbonization organic matter;0.005~0.01 part of wood vinegar;Natural minerals element 0.01~
0.02 part;0.0001~0.0002 part of agricultural grade chitosan oligosaccharide;0.01~0.02 part of agricultural grade microelement;Plant extracts 0.01
~0.02 part;
The parts by weight of the function bacterium refer to: saccharomycete: 0.001~0.005 part;Lactic acid bacteria: 0.001~0.005 part;It is withered
Careless bacillus: 0.001~0.005 part;Bacillusmusilaginosiengineering: 0.001~0.005 part;Dipel: 0.001~
0.005 part;Kazakhstan thatch rasp berry: 0.001~0.005 part;Healthy and free from worry rasp berry: 0.001~0.005 part;
The Chinese medicine parts by weight refer to: Chinese prickly ash: 0.5~1 part;Azedarach: 0.5~1 part;Honeysuckle: 0.5~1
Part;Aloe: 0.5~1 part;
The fruits and vegetables parts by weight refer to: 0.5~1 part of jujube;0.5~1 part of apple;0.5~1 part of tomato;Ginger 0.5~1
Part;0.5~1 part of garlic;
The vegetable castoff parts by weight refer to: 1~2 part of tomato waste;1~2 part of capsicum waste;Cabbage
Waste: 1~2 part;
The carbonization organic matter refers to that the solid carbonization formed by agricultural crop straw and rice husk by imperfect combustion is organic
Object, market are purchased;
The wood vinegar refers to passing through the gas aqueous solution that imperfect combustion is formed, market purchase by agricultural crop straw and rice husk
It sets;
The natural minerals element refers to ooze mineral nutrition, and market is purchased;
The extract by marine product shell that the agricultural grade chitosan oligosaccharide refers to, market is purchased;
The plant extracts refers to matrine, and market is purchased;
The parts by weight of the agricultural grade microelement refer to: ferrous sulfate: 0.001~0.003 part;Copper sulphate: 0.001~
0.003 part;Zinc sulfate: 0.001~0.003 part;Manganese sulfate: 0.001~0.003 part;Molybdenum: 0.001~0.003 part;Boron:
0.001~0.003 part.
2. the production and application method of the antibacterial insect prevention ecological system formulation of biology described in claim 1, it is characterised in that by such as
Under step carry out:
(1) Chinese medicine biologic ferment fermentation raw material and formula: Chinese prickly ash: 0.5~1 part;Azedarach: 0.5~1 part;Honeysuckle: 0.5
~1 part;Aloe: 0.5~1 part;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(2) biological fruit and vegetable ferment fermentation raw material and formula: 0.5~1 part of jujube;0.5~1 part of apple;0.5~1 part of tomato;Ginger
0.5~1 part;0.5~1 part of garlic;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(3) vegetable castoff biologic ferment fermentation raw material and formula: 1~2 part of tomato waste;1~2 part of capsicum waste;
The waste of cabbage: 1~2 part;Water: 3~5 parts;Sugar: 0.3~0.5 part;0.001~0.01 part of function bacterium, wherein saccharomycete:
0.0005~0.001 part;Lactic acid bacteria: 0.0005~0.001 part;Bacillus subtilis: 0.0005~0.001 part;Gel-shaped bud
Spore bacillus: 0.0005~0.001 part;Dipel: 0.0005~0.001 part;Kazakhstan thatch rasp berry: 0.0005~0.001 part;Health
Peaceful rasp berry: 0.0005~0.001 part;
(4) it Chinese medicine fermentation process: chooses high-quality Chinese medicine → being cut into segment → and is respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(5) fruits and vegetables fermentation process: high-quality fruits and vegetables → clean, be cut into small pieces → are chosen and are respectively put into the white modeling cleaned and sterilized
Brown sugar → addition function is added in → clear water → 200ppm chlorine dioxide 5 hours is intermittent to sterilize twice and place 24 hours → in charging basket
Energy fermenting microbe → lid lid sealing individually fermentation, fermentation time 1 month;
(6) vegetable castoff fermentation process: high-quality vegetable castoff fruits and vegetables → clean, be cut into segment → are chosen and are respectively put into
Clean and in the white plastic barrel that sterilizes → clear water → 200ppm chlorine dioxide 5 hours it is intermittent sterilize twice and place 24 hours plus
Enter brown sugar → addition function fermenting microbe → lid lid sealing individually to ferment, fermentation time 1 month;
(7) filter mixed fermentation: filtered fluid presses 1:1:1 after Chinese medicine, fruits and vegetables and vegetable castoff fermentation are filtered for 1 month respectively
Continue fermentation 1 month after mixing;
(8) carbonization organic matter is added: 0.05~0.1 part being added after filtering mixed fermentation;
(9) wood vinegar is added: 0.005~0.01 part being added after filtering mixed fermentation;
(10) natural minerals element is added: 0.01~0.02 part of natural minerals element being added after filtering mixed fermentation;
(11) agricultural grade chitosan oligosaccharide is added: 0.0001~0.0002 part of agricultural grade chitosan oligosaccharide being added after filtering mixed fermentation;
(12) agricultural grade microelement is added: 0.01~0.02 part of agricultural grade microelement being added after filtering mixed fermentation;
(13) plant extracts is added: 0.01~0.02 part of plant extracts being added after filtering mixed fermentation;
(14) fermented quality: living bacteria count 2 × 108A/L, proteinase activity 50U/L, polysaccharide 20g/L, amino acid reach 50g/
L, microelement reach 50g/L;
(15) application method: the dosage of 1 kilogram of 300-500 times of dilution, base are applied, plough deeply and bury after being manured into soil per acre;Spray
It applies, is sprayed on blade.
3. application of the antibacterial insect prevention preparation of biology described in claim 1 in terms of improving cucumber anti-efficiency.
4. application of the antibacterial insect prevention preparation of biology described in claim 1 in terms of reducing cucumber trialeurodes vaporariorum incidence.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910215886.4A CN109820005A (en) | 2019-03-21 | 2019-03-21 | Biological antibacterial insect-prevention ecological system preparation and preparation method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910215886.4A CN109820005A (en) | 2019-03-21 | 2019-03-21 | Biological antibacterial insect-prevention ecological system preparation and preparation method and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN109820005A true CN109820005A (en) | 2019-05-31 |
Family
ID=66871024
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910215886.4A Pending CN109820005A (en) | 2019-03-21 | 2019-03-21 | Biological antibacterial insect-prevention ecological system preparation and preparation method and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109820005A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111004760A (en) * | 2020-01-07 | 2020-04-14 | 山东农业大学 | Bacillus thuringiensis and application thereof |
CN112679281A (en) * | 2020-12-23 | 2021-04-20 | 金维典 | Ecological medical plant enzyme and preparation method and application thereof |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101857500A (en) * | 2010-06-21 | 2010-10-13 | 山东宝源生物有限公司 | Microbe organic fertilizer containing chelated microelements and production method thereof |
CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
CN103966148A (en) * | 2014-06-03 | 2014-08-06 | 天津市农业资源与环境研究所 | Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent |
CN105165913A (en) * | 2015-10-22 | 2015-12-23 | 苏州科大微龙农业科技有限公司 | Sophocarpidine compound prevention and treatment agent for preventing and treating trialeurodes vaporariorum |
CN105754608A (en) * | 2016-03-28 | 2016-07-13 | 鹤壁市人元生物技术发展有限公司 | Microbial soil conditioner and preparation method thereof |
CN108285397A (en) * | 2017-01-10 | 2018-07-17 | 刘永先 | A kind of pure grain fermenting organism organic fungi-manure and preparation method thereof |
CN108821873A (en) * | 2018-08-02 | 2018-11-16 | 廊坊达能新能源技术股份有限公司 | Rice hull carbon base manure material and preparation method thereof and its application in Rice Cropping |
-
2019
- 2019-03-21 CN CN201910215886.4A patent/CN109820005A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101857500A (en) * | 2010-06-21 | 2010-10-13 | 山东宝源生物有限公司 | Microbe organic fertilizer containing chelated microelements and production method thereof |
CN103300069A (en) * | 2013-05-22 | 2013-09-18 | 江苏省绿盾植保农药实验有限公司 | Biological bactericide composition and application thereof to wilt |
CN103966148A (en) * | 2014-06-03 | 2014-08-06 | 天津市农业资源与环境研究所 | Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent |
CN105165913A (en) * | 2015-10-22 | 2015-12-23 | 苏州科大微龙农业科技有限公司 | Sophocarpidine compound prevention and treatment agent for preventing and treating trialeurodes vaporariorum |
CN105754608A (en) * | 2016-03-28 | 2016-07-13 | 鹤壁市人元生物技术发展有限公司 | Microbial soil conditioner and preparation method thereof |
CN108285397A (en) * | 2017-01-10 | 2018-07-17 | 刘永先 | A kind of pure grain fermenting organism organic fungi-manure and preparation method thereof |
CN108821873A (en) * | 2018-08-02 | 2018-11-16 | 廊坊达能新能源技术股份有限公司 | Rice hull carbon base manure material and preparation method thereof and its application in Rice Cropping |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111004760A (en) * | 2020-01-07 | 2020-04-14 | 山东农业大学 | Bacillus thuringiensis and application thereof |
CN112679281A (en) * | 2020-12-23 | 2021-04-20 | 金维典 | Ecological medical plant enzyme and preparation method and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
CN100396652C (en) | Biological orgamic fertilizer and prepn thereof | |
CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
CN102964178A (en) | Composite microbial bactericide, and preparation method and application thereof | |
CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
CN102731188A (en) | Plant essence enzyme and preparation method thereof | |
CN102391876A (en) | Composite biological soil modifier and application thereof | |
CN104911129B (en) | The Bacillus amyloliquefaciens strain and its microbial organic fertilizer of control of plant bacterial wilt | |
CN109306328A (en) | Saline-alkali tolerant bacterial manure microorganism be separately cultured and serial bacterial fertilizer products production technology | |
CN109734524A (en) | Biological enzyme ecological system fertilizer and preparation method and application thereof | |
CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
CN111793498A (en) | Microbial agent and preparation method and application thereof | |
CN107032886A (en) | A kind of selenium-rich natural and multi-functional foliar fertilizer and preparation method thereof | |
CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
CN108633625B (en) | Method for preparing agaricus bisporus culture medium by taking pleurotus eryngii fungus chaff as main raw material | |
CN112047785A (en) | Chitin-containing environment-friendly enzyme biological fertilizer and preparation method thereof | |
CN113862189A (en) | Corn growth promoting, probiotic and biocontrol bacterial fertilizer integrated preparation and preparation method thereof | |
CN110432294A (en) | A kind of bacteriostatic agent of biofermentation, preparation method and application | |
CN108633626B (en) | Method for preparing agaricus bisporus culture medium by taking cowshed packing as main raw material | |
CN109438100A (en) | A kind of preparation method of the dedicated stress-resistant seaweed water-soluble fertilizer of fruits and vegetables | |
CN111777445A (en) | Method for preparing liquid organic fertilizer by biological fermentation of domestic organic wet garbage | |
CN104152149A (en) | Novel multifunctional composite biological soil improvement agent | |
CN106146194A (en) | A kind of anti-continuous cropping microbial inoculum | |
CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
CN109836298A (en) | A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Applicant after: Tianjin Academy of Agricultural Sciences Address before: 300384 microbiology laboratory, Tianjin Institute of agricultural resources and environment, 17 km away from Jinjing highway, Xiqing District, Tianjin Applicant before: TIANJIN INSTITUTE OF AGRICULTURAL RESOURCES AND ENVIRONMENT SCIENCE |
|
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190531 |