CN102038007B - Sarmentose pepper herb extract and application thereof in preparing insecticidal bacteriostat - Google Patents
Sarmentose pepper herb extract and application thereof in preparing insecticidal bacteriostat Download PDFInfo
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- CN102038007B CN102038007B CN2009102358119A CN200910235811A CN102038007B CN 102038007 B CN102038007 B CN 102038007B CN 2009102358119 A CN2009102358119 A CN 2009102358119A CN 200910235811 A CN200910235811 A CN 200910235811A CN 102038007 B CN102038007 B CN 102038007B
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Abstract
The invention discloses a sarmentose pepper herb extract and application thereof in preparing an insecticidal bacteriostat. A method for preparing the sarmentose pepper herb extract comprises the following steps of: 1) crushing roots and stems of sarmentose pepper herb to obtain coarse powder of the sarmentose pepper herb; and 2) performing cold extraction by using organic solvent in an amount which is 6 to 10 times the weight of the crushed sarmentose pepper herb, filtering and concentrating the filtrate into paste, introducing into a chromatographic column with a silica gel filler with the granularity of 200 to 300 meshes, eluting by using petroleum ether, and concentrating and drying the eluent to prepare the sarmentose pepper herb extract, wherein the time for cold extraction is 7 to 10 days and the extracting times is 2 to 3 times. The extract serving as a raw material is reasonably mixed with a solvent, an emulsifier, an antifreeze agent, a stabilizing agent and water, and sarmentose pepper herb microemulsion can be prepared. The invention has good effect of preventing and controlling prodenia litura, parasaissetia nigra nietner, lipaphis erysimi, banana anthrax bacteria and rubber anthrax bacteria. The preparation is simple in preparation process and low in cost, meets the conventional development tendency of pesticides and is suitable for popularization and application.
Description
Technical field
The present invention relates to a kind of Jia Betel extract and the application in preparing the killing pests and suppressing bacteria agent thereof.
Background technology
In the long-term struggle process of the mankind and insect, pesticide development has been experienced one from high malicious to low toxicity, poor efficiency to process efficient, that kill control, and the kind of insecticide is increasingly abundant, by its source, can roughly be divided into the four large classes such as chemosynthesis, animal sources, plant resource, microbial source.Mainly with chemical insecticide, prevent and treat at present.But, due to a large amount of uses of chemical pesticide, add that medication is lack of standardization, cause insect to produce pesticide resistance to various medicaments such as organic phosphor, carbamate, pyrethroid, nicotinoids.
Plant is the huge treasure-house of natural products, the secondary metabolites of its generation such as alkaloid, flavones, lignin, terpene, naphthoquinones class etc. are over 400,000 kinds, have in these secondary metabolitess many kinds of substance to insect have poisoning, avoid keep away, the effect such as food refusal, inhibition are grown, add the easily degraded disappearance in environment of these materials, the environment concordance is good, easily with other prophylactico-therapeutic measuress, coordinates mutually, and prospect is tempting, cause the great interest of people, become one of Research Emphasis and popular domain.
False Betel (Piper sarmentosum Roxb.), have another name called and breathe out Betel, false potato, vacation and wither, and ZUANGUFENG, be Piperaceae Piper plant, is born in other moistening place, thick forest Zhong Huo village, mountain valley.Jia Betel is the perennial draft of crawling, and that rubs has fragrance.Stipes expands, and often gives birth to adventive root.The leaf alternate, closely membranous, inhale the gland point, the wealthy avette or subcircular of the leaf of bottom, long 7-14cm, wide 6-13cm, the short point of tip, base portion heart or closely cut shape, 7 of veins; The leaf on top is little, and is avette to ovum shape lanceolar; The long 1-5cm of petiole.Flower unisexuality, dioecism, without perianth; Spike; The long 1.5-2cm of male inflorescence, diameter 2-3mm, bract is oblate, diameter 0.5-0.6mm, 2 pieces, stamen; The long 6-8mm of female inflorescence, the prolongation of fruit phase reaches 2.5cm; Bract is slightly large, column cap 3-5.Berry is subsphaeroidal, tool angle rib, and diameter 2.5-3cm, the bottom embedding is born in the order axle.Summer at florescence.Jia Betel can be used for the stomach cold pain, inflatable stomachache, and lumbago due to wind-wetness evil, postpartum, deficiency of vital energy pin was swollen, sore pain, hemorrhoid, treating swelling and pain by traumatic injury.But make up to now and find no take false Betel as raw material, the report of vegetative insecticidal bactericide.
Summary of the invention
The object of the present invention is to provide a kind of Jia Betel extract and the application in the killing pests and suppressing bacteria agent thereof.
The preparation method of Jia Betel extract provided by the present invention, that Jia Betel tissue is extracted at 20-35 ℃ of lower cold soaking with ethanol, then filter filtrate is concentrated into paste, then cross take the chromatographic column of size as 200-300 purpose silica gel particle as filler, use the benzinum wash-out, the concentrated oven dry of eluent is obtained Jia Betel extract.
In described method, also comprise described Jia Betel is organized in before extracting with the ethanol cold soaking and first dries in the shade, extract material as Jia Betel after then pulverizing 40 mesh sieves; Described Jia Betel is organized as the root of Jia Betel and/or the stem of Jia Betel.
In described method, the time that described ethanol cold soaking extracts is 7-10 days, is preferably 10 days, and the number of times of extraction is 2-3 time, is preferably 3 times.
In described method, the mass ratio that described ethanol and described Jia Betel extract material is (6-10): 1.
Said method prepares De Jia Betel extract and also belongs to the present invention's scope required for protection.
The present invention also provides a kind of pesticide-germicide microemulsion, and it is comprised of the component of following weight percentage:
Wherein, described solvent is selected from one or more any combination in ethylene glycol, cyclohexanone, dimethyl formamide, dimethylbenzene, methyl alcohol, ethanol, methyl-sulfoxide, n-butanol and benzene; Described emulsifier is selected from one or more any combination in dodecyl benzene sulfonate, castor oil oxirane ether, phenol derivatives polyoxyethylene ether, polyoxyethylene nonylphenol ether and alkylphenol polyoxyethylene; Described stabilizing agent is selected from butyl glycidyl ether, phenyl glycidyl ether, cresyl glycidyl ether, one or more any combination of polyethylene glycol diglycidyl ether etc.; Described antifreeze is selected from one or more any combination in cyclohexanone, ethylene glycol, propane diols, glycerine or PEG400; Described wet concentration is from deionized water, distilled water or running water.
Described pesticide-germicide microemulsion preferably is comprised of the component of following weight percent:
Wherein, described solvent is one or more any mixing in ethanol, n-butanol, methyl-sulfoxide, methyl alcohol and benzene, described emulsifier is one or more any mixing in dodecyl benzene sulfonate, alkylphenol polyoxyethylene, polyoxyethylene nonylphenol ether and phenol derivatives APEO, described stabilizing agent is butyl glycidyl ether and/or cresyl glycidyl ether, and described antifreeze is glycerine and/or propane diols.
Described pesticide-germicide microemulsion is particularly preferably by following 1) or 2) component of described weight percentage forms:
1）
Wherein, solvent is the mixed solution of ethanol, n-butanol and benzene, the mass ratio of ethanol, n-butanol and benzene is 3:1:1, emulsifier is the mixture of dodecyl benzene sulfonate and phenol derivatives APEO, the mass ratio of dodecyl benzene sulfonate and phenol derivatives APEO is 1:2, stabilizing agent is butyl glycidyl ether, and antifreeze is glycerine;
2）
Wherein, solvent is the mixed solution of methyl alcohol and methyl-sulfoxide, the mass ratio of methyl alcohol and methyl-sulfoxide is 9:1, emulsifier is the mixture of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether, the mass ratio of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether is 2:3, stabilizing agent is cresyl glycidyl ether, and antifreeze is propane diols.
The manufacture method of above-mentioned pesticide-germicide microemulsion, for first, Jia Betel extract is dissolved in solvent claimed in claim 7, add emulsifier claimed in claim 7, antifreeze and dispersant, stir, add water under stirring state, stir 30-60 minute, be cooled to room temperature, obtain the pesticide-germicide microemulsion.
Method of the present invention is take Jia Betel root, stem as raw material, cold soaking extracts and obtains Jia Betel extract, and with reasonable preparations such as itself and solvent, insecticides adjuvants, obtain insecticidal activity significantly, low toxicity and low residue, insect is difficult for its novel plant source pesticide-germicide microemulsion that develops immunity to drugs.
Experimental results show that, the present invention by pot experiment and field plot trial checking Jia Betel extract to prodenia litura insecticidal activity, find that this plant extracts has good control efficiency to prodenia litura, the secondary pearl lecanium of rubber, aphid, Glorosprium musarum Cookeet Mass, Colletotrichum capsici, Colletotrichum gloeosporioides Penz in Mango and rubber anthrax bacteria.The microemulsion of Yi Jia Betel extract preparation, have good resistance activity by indoor and field trial proof Jia Betel microemulsion to various insects and germ, and prodenia litura, the secondary pearl lecanium of rubber and aphid etc. are had insecticidal action preferably.The vegetable source pesticide bacteriostatic agent of Yi Jia Betel extract preparation, it is efficient, the characteristics of low toxicity meet the demand of contemporary agricultural chemicals; Have free from environmental pollution, safety, low toxicity, do not injure the characteristics such as natural enemy and non-target organism, very large in integrated pest control (IPM) potentiality, have very wide prospect; And this insecticide manufacturing process is simple, and raw material is easy to get, and is with low cost, meets the development trend of present agricultural chemicals, is suitable for applying.
Embodiment
In following embodiment, if no special instructions, be conventional method.
In following embodiment, described percentage composition if no special instructions, is the quality percentage composition.
when the present inventor passes through the screening of the multiple tropical plants insecticidal activity in Hainan Island, find that first Jia Betel has good insecticidal activity to Spodoptera litura larvae, multiple tropical germ also there is good inhibitory action, find again that afterwards this Plant Extracts is to radish aphid, the banana aphid, diamond-back moth, the various pests such as cabbage caterpillar have obvious toxic action, and by pot experiment and field plot trial checking Jia Betel extract to prodenia litura insecticidal activity, find that this plant extracts has good control efficiency to prodenia litura, when for examination concentration, being 2%, after medicine, the control efficiency of 5 days is higher than 1000 times of dilutions of contrast medicament 1.8% Avermectin.Given this, the root take Jia Betel of the present invention and stem are raw material, after extracting by organic solvent, with extract obtained, with insecticides adjuvant, rationally mix, and make a kind of vegetative insecticidal bactericidal microemulsion., take following embodiment as example, the preparation of false Betel extract of the present invention is described and with it, prepares the effect of insecticide.
Below the present invention is described in further detail.
The preparation of embodiment 1, Jia Betel extract
The false Betel plant (nursery stock is available from Bozhou City Bao Hua pharmaceutcal corporation, Ltd) that grows to the period of blooming is cleaned up, and its leaf reject is fallen, after being put in Indoor Natural and drying in the shade, its root and stem were pulverized 40 mesh sieves,, take ethanol as solvent, in the ratio that Jia Betel and the weight ratio of ethanol are 1:8, mixed, cold soaking is (under room temperature, 20-35 ℃, soak) extract each 10 days 3 times; Filter with Buchner funnel, merging filtrate, with Rotary Evaporators reduced pressure concentration filtrate to paste, the weight 100-200 order column chromatography silica gel such as add to mix powder, adopt dry method upper prop (filler of chromatographic column is 200-300 purpose column chromatography silica gel), carry out wash-out take benzinum as solvent, the eluent of collecting is dried De Jia Betel extract after concentrated in 45 ℃ of electric heating constant-temperature blowing drying boxes again.
The preparation of embodiment 2, Jia Betel extracts
The false Betel plant (nursery stock is available from Bozhou City Bao Hua pharmaceutcal corporation, Ltd) that grows to the period of blooming is placed in Indoor Natural dries in the shade and pulverized 40 mesh sieves; with 10 times of methyl alcohol to Jia Betel crushed material weight; under refluxad extract; extract temperature 60 C; 24 hours extraction times;, to paste, then dry to constant weight De Jia Betel extract with Rotary Evaporators reduced pressure concentration filtrate in 45 ℃ of electric heating constant-temperature blowing drying boxes.
The preparation of embodiment 3,5% Jia Betel microemulsion
5% Jia Betel microemulsion is comprised of the material of following weight percent:
Wherein, solvent is the mixed solution of ethanol, n-butanol and benzene, the mass ratio of ethanol, n-butanol and benzene is 3:1:1, emulsifier is the mixture of dodecyl benzene sulfonate and phenol derivatives APEO, the mass ratio of dodecyl benzene sulfonate and phenol derivatives APEO is 1:2, stabilizing agent is butyl glycidyl ether, and antifreeze is glycerine.
Take 5 kilograms of embodiment 1 preparation De Jia Betel extracts, be dissolved in the mixed solution (mass ratio of ethanol, n-butanol and benzene is 3:1:1) of 15 kilograms of ethanol, n-butanol and benzene, then add successively 6 kilograms of calcium dodecyl benzene sulfonates (DBS-Ca) and 12 kilograms of phenol derivatives APEOs (1601, this is the title of emulsifier), 5 kilograms of glycerine and 3 kilograms of butyl glycidyl ethers, stir under room temperature, then add running water to complement to 100 kilograms under high-speed stirred, stirred again 45 minutes, cooling, obtain 5% Jia Betel pesticide-germicide microemulsion.
The preparation of embodiment 4,10% Jia Betel microemulsion
10% Jia Betel microemulsion is comprised of the material of following weight percent:
Wherein, solvent is the mixed solution of methyl alcohol and methyl-sulfoxide, the mass ratio of methyl alcohol and methyl-sulfoxide is 9:1, emulsifier is the mixture of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether, the mass ratio of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether is 2:3, stabilizing agent is cresyl glycidyl ether, and antifreeze is propane diols.
Take 10 kilograms of embodiment 1 preparation De Jia Betel extracts, be dissolved in the mixed solution (mass ratio of methyl alcohol and methyl-sulfoxide is 9:1) of 20 kilograms of methyl alcohol and methyl-sulfoxide, then add successively 8 kilograms of alkylphenol polyoxyethylene (OP-10) and 12 kilograms of polyoxyethylene nonylphenol ether (NP-15), 2 kilograms of propane diols, 2.5 kilogram cresyl glycidyl ether, stir under room temperature, then add running water to supply 100 kilograms under high-speed stirred, stirred again 45 minutes, cooling, obtain 10% Jia Betel microemulsion.
Embodiment 5, Jia Betel microemulsion insecticidal bacteriostatic activities
1 for the examination material
1) for the examination insect
Prodenia litura (Spodoptera litura Fabricius): pick up from the pawpaw ground of not dispenser, in indoor feeding many generations, during test picking in the same size, bouncing 3 mid-terms in age larva for examination.
The secondary pearl lecanium of rubber (Parasaissetia nigra) picks up from the rubber ground of not dispenser, and in indoor feeding many generations, test is selected and tried worm 3 ages of the same size for trying.
Radish aphid (Lipaphiserysimi pseudobrassicae) is picked up from Research Institute of Environment and Plant Protection, Chinese Academy of Tropi proving ground, and the adult of adopting is back tested.
2) for the examination bacterial classification
Glorosprium musarum Cookeet Mass (Colletotrichum musae) (buying from Chinese agriculture microorganism fungus kind preservation administrative center, deposit number ACCC No.31247);
Colletotrichum capsici (Colletotrichum capsici) (Chinese agriculture microorganism fungus kind preservation administrative center, deposit number ACCC No.31218);
Colletotrichum gloeosporioides Penz in Mango (Colletotrichum gloeosporioides) (buying from Chinese agriculture microorganism fungus kind preservation administrative center, deposit number ACCC No.31219);
Rubber anthrax bacteria (Colletotrichum gloeosporioules) (buying in Chinese agriculture microbial strains preservation administrative center, deposit number ACCC No.30012).
2 test methods
The insecticidal activity of the insecticidal bactericide of 1) Jia Betel extract and preparation thereof detects
Adopt infusion process measure (Mu Liyi. the chemical protection of plant research method. the .1987:56 of Chinese agriculture publishing house) measure the cytotoxicity of 10% false Betel microemulsion of the 5% false Betel microemulsion of false Betel extract (false Betel extract 2), embodiment 3 preparations of false Betel extract (false Betel extract 1), embodiment 2 preparations of embodiment 1 preparation and embodiment 4 preparations to prodenia litura; Wherein, embodiment 1 preparation De Jia Betel extract 1 and embodiment 2 preparation De Jia Betel extracts 2 are settled to the 50mg/ml mother liquor with acetone god respectively, above four kinds of medicaments are respectively with containing 0.1%(quality percentage composition) distilled water solution of Tween 80 is diluted to that to contain effective composition (being false Betel extract) concentration be that 1mg/mL or 0.5mg/mL are standby, every concentration repeats 4 times, 15 of every repetitions examination worms, use contain 0.1% Tween 80 distilled water as blank.
With semar technique (Bi Renjun. the development .2009:20 of the false Betel microemulsion of botanical pesticide and mixture preparation) measure the cytotoxicity of 10% false Betel microemulsion of the 5% false Betel microemulsion of false Betel extract 2, embodiment 3 preparations of false Betel extract 1, embodiment 2 preparations of embodiment 1 preparation and embodiment 4 preparations to the secondary pearl lecanium of rubber third-instar larvae; Wherein, embodiment 1 preparation De Jia Betel extract 1 and embodiment 2 preparation De Jia Betel extracts 2 are settled to the 50mg/ml mother liquor with acetone god respectively, above four kinds of medicaments are respectively with containing the 0.1%(mass percentage concentration) distilled water solution of Tween 80 is diluted to that to contain effective composition (being false Betel extract) concentration be that 1mg/mL or 0.5mg/mL are standby, every concentration repeats 4 times, 15 of every repetitions examination worms, use contain 0.1% Tween 80 distilled water as blank.
With spray-on process (Mu Liyi. the chemical protection of plant research method. the .1987:56-58 of Chinese agriculture publishing house) measure the cytotoxicity of 10% false Betel microemulsion of the 5% false Betel microemulsion of false Betel extract 2, embodiment 3 preparations of false Betel extract 1, embodiment 2 preparations of embodiment 1 preparation and embodiment 4 preparations to radish aphid; Wherein, embodiment 1 preparation De Jia Betel extract 1 and embodiment 2 preparation De Jia Betel extracts 2 are settled to the 50mg/ml mother liquor with acetone god respectively, it is that 1mg/mL and 0.5mg/mL are standby that above four kinds of medicaments use respectively the distilled water solution that contains 0.1% Tween 80 to be diluted to contain effective composition (being false Betel extract) concentration, every concentration repeats 4 times, 15 of every repetitions examination worms, use contain 0.1% Tween 80 distilled water as blank.
Lethality (%)=dead borer population/total borer population * 100
Corrected mortality (%)=(processing lethality-contrast lethality)/(100-contrasts lethality) * 100
The insecticidal activity result of the insecticidal bactericide of Jia Betel extract and preparation thereof is as shown in table 1, result shows, 5% Jia Betel microemulsion and 10% Jia Betel microemulsion are to prodenia litura 3 instar larvaes, secondary pearl lecanium 3 instar larvaes of rubber and the very strong cytotoxicity of radish aphid tool, and its insecticidal activity intensity will be higher than equal in quality concentration De Jia Betel extract.
The insecticidal activity of the insecticidal bactericide of table 1 Jia Betel extract and preparation thereof to several insects
The bactericidal activity of the insecticidal bactericide of 2) Jia Betel extracts and preparation thereof detects
Adopt growth rate method (Mu Liyi. the chemical protection of plant research method. the .1987:79-81 of Chinese agriculture publishing house) measure the inhibition mycelial growth effect of 10% false Betel microemulsion of the 5% false Betel microemulsion of false Betel extract (false Betel extract 2), embodiment 3 preparations of false Betel extract (false Betel extract 1), embodiment 2 preparations of embodiment 1 preparation and embodiment 4 preparations to Glorosprium musarum Cookeet Mass, Colletotrichum capsici, Colletotrichum gloeosporioides Penz in Mango, rubber anthrax bacteria; Wherein, embodiment 1 preparation De Jia Betel extract 1 and embodiment 2 preparation De Jia Betel extracts 2 are settled to respectively the mother liquor of 50mg/ml with acetone, above four kinds of medicaments are respectively with containing the 0.1%(mass percentage concentration) distilled water solution of Tween 80 is diluted to that to contain effective composition (being false Betel extract) concentration be that 0.5mg/mL and 0.1mg/mL are standby, every concentration repeats 3 times, 3 bacterium cakes of every repetition (are measured for examination fungus colony growth diameter with the right-angled intersection method, get its mean value), use contain 0.1% Tween 80 distilled water as blank.(purity is carbendazim, doctor's prestige agricultural chemicals company provides by Hainan) former medicinal acetone is settled to the mother liquor of 50mg/ml, uses the 0.1%(mass percentage concentration) distilled water solution of Tween 80 be diluted to contain effective composition (carbendazim) concentration be 0.5 for 0.5mg/mL and 0.1mg/mL standby.
Inhibiting rate (%)=(contrast colony diameter-processing colony diameter)/contrast colony diameter * 100
The bactericidal activity result of the insecticidal bactericide of Jia Betel extract and preparation thereof is as shown in table 2, result shows, 5% Jia Betel microemulsion and 10% Jia Betel microemulsion are to Glorosprium musarum Cookeet Mass, rubber anthrax bacteria and the very strong inhibitory action of Colletotrichum gloeosporioides Penz in Mango tool, especially to the rubber anthrax bacteria, its bacteriostatic activity under equal in quality concentration will be higher than contrast medicament carbendazim and extract (Jia Betel extract 1 He Jia Betel extract 2) contrast.
The inhibitory action of the insecticidal bactericide of table 2 Jia Betel extract and preparation thereof to the several plant disease fungus
The field insecticidal activity test of the insecticidal bactericide of 3) Jia Betel extracts and preparation thereof to prodenia litura:
10% Jia Betel microemulsion of 5% Jia Betel microemulsion of 1 reagent agent: embodiment 3 preparation, embodiment 4 preparations, with 1.8% abamectin emulsifiable concentrate (Hainan doctor's prestige agricultural chemicals company), embodiment 1 preparation De Jia Betel extract and embodiment 2 preparation De Jia Betel extracts.
2 for studying thing and controlling object: Chinese cabbage, prodenia litura.
3 test methods
Giving birth to reference to the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC the pesticide field efficacy medicine test criterion of surveying the chamber formulation carries out.Carry out in Danzhou, Hainan Shatian group in November, 2008, selects the higher Chinese cabbage ground of insect density to test.if 5% Jia Betel microemulsion and 10% Jia Betel microemulsion be 500 times of volumes of dilute with water and 1000 times of volumes respectively, 1.8% 2000 times of abamectin emulsifiable concentrate dilute with waters volume, with embodiment 1 preparation De Jia Betel extract (Jia Betel extract 1) and embodiment 2 prepare De Jia Betel extract (Jia Betel extracts 2) make respectively the mother liquor of 100mg extract/ml with acetone solution, then mix for the ratio of 10:1 and Tween-80 by volume, until after complete emulsification, be diluted with water to 500 times of volumes of mother liquor as extract medicament (in liquid, the content of Tween-80 is no more than 0.05%), take clear water as contrast (CK), totally 8 processing.3 repetitions are established in every processing, totally 24 residential quarters.Medicament is diluted to desired concn, till then with hand-held compression sprayer, being sprayed to leave dual sides and all liquid being arranged.Choose at random the investigation of 10-15 strain Chinese cabbage before dispenser.The insect population radix of investigation prodenia litura before dispenser.1d after the spray medicine, 2d, 3d investigate remaining borer population alive on the Chinese cabbage of mark, calculate insect population decline rate and the preventive effect of each treatment region.
Insect population radix * 100 before insect population decline rate (%)=(insect population radix after the front insect population radix-dispenser of dispenser)/dispenser
Control efficiency (%)=(processing insect population decline rate-contrast insect population decline rate)/(100-contrast insect population decline rate) * 100
Field test results is as shown in table 3, and result shows that Jia Betel microemulsion has control efficiency preferably to prodenia litura, will contrast higher than extract under equal in quality concentration, and is suitable with the control efficiency of 1.8% abamectin emulsifiable concentrate.
The microemulsion of table 3 Jia Betel extract and preparation thereof is to the prodenia litura field control effectiveness test
Claims (8)
1. pesticide-germicide microemulsion, described pesticide-germicide microemulsion is comprised of the component of following weight percent:
Jia Betel extract 5-12.5%,
Solvent 15-20%,
Emulsifier 18-20%,
Stabilizing agent 2.5-3%,
Antifreeze 2-5%,
Surplus is water;
Wherein, described solvent is one or more any mixing in ethanol, n-butanol, methyl-sulfoxide, methyl alcohol and benzene, described emulsifier is one or more any mixing in dodecyl benzene sulfonate, alkylphenol polyoxyethylene, polyoxyethylene nonylphenol ether and phenol derivatives APEO, described stabilizing agent is butyl glycidyl ether and/or cresyl glycidyl ether, and described antifreeze is glycerine and/or propane diols;
The preparation method of described Jia Betel extract, that Jia Betel tissue is extracted at 20-35 ℃ of lower cold soaking with ethanol, then filter filtrate is concentrated into paste, then cross take the chromatographic column of size as 200-300 purpose silica gel particle as filler, use the benzinum wash-out, the concentrated oven dry of eluent is obtained Jia Betel extract.
2. pesticide-germicide microemulsion according to claim 1 is characterized in that: the preparation method of described Jia Betel extract also comprises described Jia Betel is organized in before extracting with the ethanol cold soaking and first dries in the shade, and extracts material as Jia Betel after then pulverizing 40 mesh sieves; Described Jia Betel is organized as the root of Jia Betel and/or the stem of Jia Betel.
3. pesticide-germicide microemulsion according to claim 2 is characterized in that: in the preparation method of described Jia Betel extract, the time that described ethanol cold soaking extracts is 7-10 days, and the number of times of extraction is 2-3 time.
4. pesticide-germicide microemulsion according to claim 3 is characterized in that: in the preparation method of described Jia Betel extract, the time that described ethanol cold soaking extracts is 10 days, and the number of times of extraction is 3 times.
5. pesticide-germicide microemulsion according to claim 3 is characterized in that: the mass ratio that described ethanol and described Jia Betel extract material is (6-10): 1; Described Jia Betel is organized as root and/or the stem that grows to the De Jia Betel in period of blooming.
6. the described pesticide-germicide microemulsion of any one according to claim 1-5, described pesticide-germicide microemulsion is by following 1) or 2) component of described weight percentage forms:
1）
Jia Betel extract 5%,
Solvent 15%,
Emulsifier 18%,
Stabilizing agent 3%,
Antifreeze 5%,
Surplus is water;
Wherein, solvent is the mixed solution of ethanol, n-butanol and benzene, the mass ratio of ethanol, n-butanol and benzene is 3:1:1, emulsifier is the mixture of dodecyl benzene sulfonate and phenol derivatives APEO, the mass ratio of dodecyl benzene sulfonate and phenol derivatives APEO is 1:2, stabilizing agent is butyl glycidyl ether, and antifreeze is glycerine;
2）
Jia Betel extract 10%,
Solvent 20%,
Emulsifier 20%,
Stabilizing agent 2.5%,
Antifreeze 2%,
Surplus is water;
Wherein, solvent is the mixed solution of methyl alcohol and methyl-sulfoxide, the mass ratio of methyl alcohol and methyl-sulfoxide is 9:1, emulsifier is the mixture of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether, the mass ratio of alkylphenol polyoxyethylene and polyoxyethylene nonylphenol ether is 2:3, stabilizing agent is cresyl glycidyl ether, and antifreeze is propane diols.
7. the manufacture method of pesticide-germicide microemulsion claimed in claim 1, for first, Jia Betel extract is dissolved in solvent claimed in claim 1, add emulsifier claimed in claim 1, antifreeze and dispersant, stir, add water under stirring state, stir 30-60 minute, be cooled to room temperature, obtain the pesticide-germicide microemulsion.
8. the application of the described insecticidal bactericide of any one in pest control and/or disease in claim 1-6; Described insect pest is the insect pest that the secondary pearl lecanium of prodenia litura and/or rubber and/or radish aphid cause; Described disease is Glorosprium musarum Cookeet Mass and/or Colletotrichum capsici and/or Colletotrichum gloeosporioides Penz in Mango and/or the microbial disease of rubber anthracnose.
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| CN104771451B (en) * | 2015-04-24 | 2018-01-30 | 中国人民解放军第二军医大学 | A kind of Chinese medical extract and its application with antidepressant effect |
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| CN107372621A (en) * | 2017-07-20 | 2017-11-24 | 陈超 | A kind of mulberry tree Pesticidal combination to silkworm safety |
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| CN107307007A (en) * | 2017-07-31 | 2017-11-03 | 宾阳禾医生农业服务有限公司 | A kind of Pesticidal combination for preventing and treating mulberry cecidomyiia |
| CN107624799A (en) * | 2017-09-08 | 2018-01-26 | 广西南宁益土生物科技有限责任公司 | One kind sterilization synergistic composition |
| CN108013079A (en) * | 2017-12-15 | 2018-05-11 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition and its application |
| CN107853331A (en) * | 2017-12-18 | 2018-03-30 | 广西南宁乐蕊生物科技有限责任公司 | bactericidal composition containing bromothalonil |
| CN108271812A (en) * | 2017-12-29 | 2018-07-13 | 广西南宁乐蕊生物科技有限责任公司 | A kind of Synergistic bactericidal composition and its application |
| US20220264897A1 (en) * | 2019-08-23 | 2022-08-25 | University Of Florida Research Foundation, Inc. | Compositions and methods relating to insecticides |
| CN113068767A (en) * | 2021-03-30 | 2021-07-06 | 中国热带农业科学院热带生物技术研究所 | Application of sarmentosum bungei extract in preparation of additive for preventing and treating avian aspergillosis |
| CN113424826B (en) * | 2021-04-28 | 2022-08-05 | 中国热带农业科学院环境与植物保护研究所 | Compound weeding composition containing sarmentine and application thereof |
| CN115624035A (en) * | 2022-10-27 | 2023-01-20 | 保亭海农农业开发有限公司 | Piper sarmentosum bunge extract and preparation method and application thereof |
| CN116889586B (en) * | 2023-05-26 | 2024-07-16 | 桂林医学院 | Application of total flavone extract of caulis et folium Piperis Kadsurae in preparing liver-protecting medicine |
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| CN1319575C (en) * | 2005-06-07 | 2007-06-06 | 广西梧州制药（集团）股份有限公司 | Compound traditional Chinese medicine for treating fracture and injury, prepn. method and use thereof |
| CN1813922A (en) * | 2005-11-28 | 2006-08-09 | 蓝子花 | Medicinal liquor of Hongheierwan for dispelling wetness-evil and stopping pain |
| WO2008100130A2 (en) * | 2007-02-16 | 2008-08-21 | Tuck Pooi Soo | Composition containing kadok extract and collagen |
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